Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 231,378 C→A 100% R26S (CGC→AGC)  yafS → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273231,3780CA98.7% 774.2 / 0.4 242R26S (CGC→AGC) yafSputative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (132/106);  total (134/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 243 total aligned reads displayed.

ATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATG  >  CP009273/231234‑231521
                                                                                                                                                |                                                                                                                                               
aTTGTCATCAAAGGCGGTAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCa                                                                                                                                              <  2:1352971/148‑1 (MQ=255)
aTTGTCATCAAAGGCGGGAATACTGTTAAGATTCATTGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCa                                                                                                                                              <  1:1010426/148‑1 (MQ=255)
aTTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCa                                                                                                                                              >  1:2465846/1‑148 (MQ=255)
   gTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAgg                                                                                                                                           <  2:421631/148‑1 (MQ=255)
     catcaAAGGCGGGAATACTGATAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGgcg                                                                                                                                         <  2:2040498/148‑1 (MQ=255)
        caAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTg                                                                                                                                      >  2:2146110/1‑148 (MQ=255)
         aaaGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTgg                                                                                                                                     <  1:171854/148‑1 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGACAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  2:2778466/1‑148 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  1:1630337/1‑148 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  1:2359108/1‑148 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  2:841208/1‑148 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  1:1630386/1‑148 (MQ=255)
             gCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgcg                                                                                                                                 >  1:3022181/1‑148 (MQ=255)
                  aaTACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGc                                                                                                                            <  1:2008735/148‑1 (MQ=255)
                   aTACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCt                                                                                                                           >  2:2884072/1‑148 (MQ=255)
                   aTACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCt                                                                                                                           <  2:2304172/148‑1 (MQ=255)
                     aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCa                                                                                                                         >  1:1400154/1‑148 (MQ=255)
                     aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCa                                                                                                                         >  2:1764891/1‑148 (MQ=255)
                     aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCa                                                                                                                         >  2:2641732/1‑148 (MQ=255)
                     aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCa                                                                                                                         >  1:473444/1‑148 (MQ=255)
                      cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCaa                                                                                                                        >  1:2664088/1‑148 (MQ=255)
                       tGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAg                                                                                                                             <  2:2106067/142‑1 (MQ=255)
                        gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAAcc                                                                                                                      <  1:440094/148‑1 (MQ=255)
                        gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAAcc                                                                                                                      <  2:1544153/148‑1 (MQ=255)
                            aGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTg                                                                                                                  >  2:2739786/1‑148 (MQ=255)
                             gATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTgg                                                                                                                 <  1:2454045/148‑1 (MQ=255)
                             gATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTgg                                                                                                                 <  1:1093180/148‑1 (MQ=255)
                                  aTAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCAc                                                                                                            <  2:2828285/148‑1 (MQ=255)
                                     gATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTaa                                                                                                         >  2:2199818/1‑148 (MQ=255)
                                      aTTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTACCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTaaa                                                                                                        <  2:1408532/148‑1 (MQ=255)
                                        tACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAt                                                                                                      >  2:2155915/1‑148 (MQ=255)
                                         aCCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATg                                                                                                     >  2:3511359/1‑148 (MQ=255)
                                          ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                    >  2:291948/1‑148 (MQ=255)
                                          ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                    >  2:3367376/1‑148 (MQ=255)
                                          ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                    <  1:2474369/148‑1 (MQ=255)
                                             ctcAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGGGGTTCACTAAAATGTATg                                                                                                 >  2:789065/1‑148 (MQ=255)
                                               cAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGt                                                                                               <  1:2614500/148‑1 (MQ=255)
                                                 gtgtGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTTGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGttt                                                                                             >  1:314704/1‑148 (MQ=255)
                                                  tgtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGtttt                                                                                            <  2:1748928/148‑1 (MQ=255)
                                                     gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAt                                                                                         <  2:49220/148‑1 (MQ=255)
                                                     gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCATGATTGCTGGGGCGATTTGCCCTGGGGAAACCTTTATAGAAAGGAGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCaa                                                                                         >  1:2066445/1‑147 (MQ=255)
                                                      aaaCGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATc                                                                                        <  1:1493444/148‑1 (MQ=255)
                                                        aCGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTg                                                                                      <  2:1120781/148‑1 (MQ=255)
                                                         cGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGc                                                                                     <  1:2150427/148‑1 (MQ=255)
                                                          ggAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCt                                                                                    <  1:891859/148‑1 (MQ=255)
                                                              ggTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAg                                                                                >  1:3368806/1‑148 (MQ=255)
                                                              ggTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAg                                                                                >  2:3269320/1‑148 (MQ=255)
                                                                 tgtgATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt                                                                             >  2:3293194/1‑148 (MQ=255)
                                                                  gtgATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTg                                                                            <  2:1446896/148‑1 (MQ=255)
                                                                   tgatgaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTATGATTgg                                                                           >  1:625729/1‑148 (MQ=255)
                                                                   tgatgaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTgg                                                                           <  1:2739786/148‑1 (MQ=255)
                                                                        aaaCCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCACtt                                                                      >  2:1935420/1‑148 (MQ=255)
                                                                        aaaCCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAtt                                                                      >  2:2553622/1‑148 (MQ=255)
                                                                           ccGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTaa                                                                   <  1:2885123/148‑1 (MQ=255)
                                                                                     tCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAt                                                         >  2:2584817/1‑148 (MQ=255)
                                                                                     tCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAt                                                         >  2:239902/1‑148 (MQ=255)
                                                                                     tCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAt                                                         >  2:14624/1‑148 (MQ=255)
                                                                                       ccTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCa                                                       <  2:1901048/148‑1 (MQ=255)
                                                                                       ccTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCa                                                       <  1:3456728/148‑1 (MQ=255)
                                                                                        cTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCaa                                                      >  2:3102444/1‑148 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     >  1:2810845/1‑148 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     <  1:1255394/148‑1 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     >  1:3274955/1‑148 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     >  1:3090983/1‑148 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     <  2:2358146/148‑1 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     <  1:304846/148‑1 (MQ=255)
                                                                                          cAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAtt                                                    >  1:2050343/1‑148 (MQ=255)
                                                                                          cAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAtt                                                    >  1:2871812/1‑148 (MQ=255)
                                                                                           aaaCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTg                                                   >  1:1943817/1‑148 (MQ=255)
                                                                                            aaCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGc                                                  >  1:704310/1‑148 (MQ=255)
                                                                                             aCTGTCGTGGCTCCTGATTGCTGGTGCGATTTGCCCTTGGGAAATCTTTATATCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCg                                                 <  2:2135876/148‑1 (MQ=255)
                                                                                               tGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGaa                                               <  1:3238698/148‑1 (MQ=255)
                                                                                               tGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGaa                                               <  1:1305179/148‑1 (MQ=255)
                                                                                                gTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAg                                              >  2:2346651/1‑148 (MQ=255)
                                                                                                     ggCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgc                                         >  1:3324694/1‑148 (MQ=255)
                                                                                                      gCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcg                                        >  2:46641/1‑148 (MQ=255)
                                                                                                      gCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcg                                        >  2:2468398/1‑148 (MQ=255)
                                                                                                         ccTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCgg                                     <  2:704256/148‑1 (MQ=255)
                                                                                                         ccTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCgg                                     <  2:2718092/148‑1 (MQ=255)
                                                                                                           tGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGtt                                   >  2:2801307/1‑148 (MQ=255)
                                                                                                              ttGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtct                                >  1:1039550/1‑148 (MQ=255)
                                                                                                                    gggCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCaa                          >  1:2285679/1‑148 (MQ=255)
                                                                                                                     ggCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAg                         >  2:682860/1‑148 (MQ=255)
                                                                                                                      gCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGt                        <  1:2155915/148‑1 (MQ=255)
                                                                                                                      gCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGt                        >  1:2590717/1‑148 (MQ=255)
                                                                                                                       cGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTg                       >  2:1593672/1‑148 (MQ=255)
                                                                                                                         aTTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGaa                     >  2:1730763/1‑148 (MQ=255)
                                                                                                                         aTTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGaa                     <  2:379839/148‑1 (MQ=255)
                                                                                                                           ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                   >  2:1432238/1‑148 (MQ=255)
                                                                                                                           ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                   >  1:197685/1‑148 (MQ=255)
                                                                                                                           ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                   <  1:3447045/148‑1 (MQ=255)
                                                                                                                            tGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAGCCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGt                  <  2:3188332/148‑1 (MQ=255)
                                                                                                                            tGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGt                  <  1:3105314/148‑1 (MQ=255)
                                                                                                                              cccTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTAGGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGttt                <  2:1958411/148‑1 (MQ=255)
                                                                                                                                 tGGGGAAAGCTCTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTg             >  2:2421/1‑148 (MQ=255)
                                                                                                                                  ggggAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTgc            >  2:2564814/1‑148 (MQ=255)
                                                                                                                                     gAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCa         >  2:3052557/1‑148 (MQ=255)
                                                                                                                                          cTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGaa    <  1:1837403/148‑1 (MQ=255)
                                                                                                                                          cTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGaa    <  2:2839308/148‑1 (MQ=255)
                                                                                                                                            ttATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATg  <  1:1876154/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATG  >  CP009273/231234‑231521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTC  >  CP009273/231229‑231527
                                                                                                                                                     |                                                                                                                                                     
ATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATA                                                                                                                                                       >  SRR19973065.1694719/1‑150 (MQ=60)
ATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATA                                                                                                                                                       >  SRR19973065.2682393/1‑150 (MQ=60)
ATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATA                                                                                                                                                       <  SRR19973065.2055354/150‑1 (MQ=60)
 TGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAG                                                                                                                                                      >  SRR19973065.2262012/1‑150 (MQ=60)
   TAATTTTCATCAAAGGCGGGAATACTGTTAAGATTAATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTACCAAAAACTGTCGTGGCTCCTGATTGATGGGGCGATTTGCCCGGGGGAAACCTTTATAGCA                                                                                                                                                    >  SRR19973065.2648927/1‑150 (MQ=60)
   TAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCA                                                                                                                                                    >  SRR19973065.3157741/1‑150 (MQ=60)
   TAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCA                                                                                                                                                    >  SRR19973065.2481387/1‑150 (MQ=60)
   TAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCA                                                                                                                                                    >  SRR19973065.2481497/1‑150 (MQ=60)
    AATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAA                                                                                                                                                   >  SRR19973065.3132048/1‑150 (MQ=60)
    AATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAA                                                                                                                                                   >  SRR19973065.3207571/1‑150 (MQ=60)
     ATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATTGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAG                                                                                                                                                  <  SRR19973065.1017126/150‑1 (MQ=60)
              AAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAG                                                                                                                                         <  SRR19973065.173230/150‑1 (MQ=60)
               AAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGC                                                                                                                                        >  SRR19973065.1641110/1‑150 (MQ=60)
               AAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGC                                                                                                                                        >  SRR19973065.1641268/1‑150 (MQ=60)
               AAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGC                                                                                                                                        >  SRR19973065.1641314/1‑150 (MQ=60)
               AAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGC                                                                                                                                        >  SRR19973065.1641317/1‑150 (MQ=60)
               AAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGC                                                                                                                                        >  SRR19973065.2373772/1‑150 (MQ=60)
                AGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCG                                                                                                                                       >  SRR19973065.3040700/1‑150 (MQ=60)
                   CGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCA                                                                                                                                    >  SRR19973065.3309534/1‑150 (MQ=60)
                    GGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAG                                                                                                                                   <  SRR19973065.2178195/150‑1 (MQ=60)
                       AATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTC                                                                                                                                <  SRR19973065.2021702/150‑1 (MQ=60)
                       AATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTC                                                                                                                                <  SRR19973065.122576/150‑1 (MQ=60)
                        ATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCA                                                                                                                               >  SRR19973065.2372804/1‑150 (MQ=60)
                        ATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCA                                                                                                                               >  SRR19973065.2374605/1‑150 (MQ=60)
                        ATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCA                                                                                                                               >  SRR19973065.3057594/1‑150 (MQ=60)
                        ATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCA                                                                                                                               <  SRR19973065.176295/150‑1 (MQ=60)
                        ATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCA                                                                                                                               >  SRR19973065.1409334/1‑150 (MQ=60)
                        ATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCA                                                                                                                               >  SRR19973065.476680/1‑150 (MQ=60)
                         TACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAA                                                                                                                              >  SRR19973065.2679984/1‑150 (MQ=60)
                         TACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAA                                                                                                                              >  SRR19973065.2680573/1‑150 (MQ=60)
                          ACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTagat                                                                                                                             >  SRR19973065.2119610/1‑146 (MQ=60)
                            TGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCC                                                                                                                           <  SRR19973065.3454023/150‑1 (MQ=60)
                             GTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCG                                                                                                                          <  SRR19973065.442893/150‑1 (MQ=60)
                             GTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCG                                                                                                                          <  SRR19973065.443144/150‑1 (MQ=60)
                             GTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCG                                                                                                                          >  SRR19973065.20592/1‑150 (MQ=60)
                                  GATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTT                                                                                                                     <  SRR19973065.1100377/150‑1 (MQ=60)
                                  GATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTT                                                                                                                     <  SRR19973065.2469559/150‑1 (MQ=60)
                                  GATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTT                                                                                                                     <  SRR19973065.1100389/150‑1 (MQ=60)
                                  GATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTT                                                                                                                     <  SRR19973065.2387789/150‑1 (MQ=60)
                                  GATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTT                                                                                                                     <  SRR19973065.2469511/150‑1 (MQ=60)
                                        TAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAA                                                                                                               >  SRR19973065.284702/1‑150 (MQ=60)
                                        TAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAA                                                                                                               >  SRR19973065.1360646/1‑150 (MQ=60)
                                           ATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAT                                                                                                            >  SRR19973065.1966938/1‑150 (MQ=60)
                                           ATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAT                                                                                                            >  SRR19973065.54689/1‑150 (MQ=60)
                                           ATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAT                                                                                                            >  SRR19973065.251082/1‑150 (MQ=60)
                                             TACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGT                                                                                                          >  SRR19973065.1710196/1‑150 (MQ=60)
                                               CCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTAT                                                                                                        <  SRR19973065.2489946/150‑1 (MQ=60)
                                               CCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTAT                                                                                                        <  SRR19973065.2490186/150‑1 (MQ=60)
                                                 TCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGG                                                                                                      <  SRR19973065.139261/150‑1 (MQ=60)
                                                  CTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGT                                                                                                     >  SRR19973065.199650/1‑150 (MQ=60)
                                                  CTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGT                                                                                                     >  SRR19973065.1485591/1‑150 (MQ=60)
                                                   TCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTT                                                                                                    >  SRR19973065.206967/1‑150 (MQ=60)
                                                    CAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTTGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTT                                                                                                   >  SRR19973065.316836/1‑150 (MQ=60)
                                                    CAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTT                                                                                                   <  SRR19973065.2630702/150‑1 (MQ=60)
                                                       TGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCA                                                                                                >  SRR19973065.447049/1‑150 (MQ=60)
                                                        GTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAT                                                                                               >  SRR19973065.2690530/1‑150 (MQ=60)
                                                        GTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAT                                                                                               >  SRR19973065.1035327/1‑150 (MQ=60)
                                                        GTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCATGATTGCTGGGGCGATTTGCCCTGGGGAAACCTTTATAGAAAGGAGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAA                                                                                               >  SRR19973065.2079612/1‑150 (MQ=60)
                                                           AAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTG                                                                                            <  SRR19973065.1503035/150‑1 (MQ=60)
                                                              CGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTT                                                                                         <  SRR19973065.2164256/150‑1 (MQ=60)
                                                               GGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTA                                                                                        <  SRR19973065.897517/150‑1 (MQ=60)
                                                                 AAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAG                                                                                      >  SRR19973065.2215926/1‑150 (MQ=60)
                                                                 AAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAG                                                                                      >  SRR19973065.3389408/1‑150 (MQ=60)
                                                                 AAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAG                                                                                      <  SRR19973065.2114786/150‑1 (MQ=60)
                                                                 AAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGAGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAG                                                                                      >  SRR19973065.2775393/1‑150 (MQ=60)
                                                                    GTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATT                                                                                   >  SRR19973065.177490/1‑150 (MQ=60)
                                                                      TGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTATGATTGG                                                                                 >  SRR19973065.629792/1‑150 (MQ=60)
                                                                        TGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCA                                                                               <  SRR19973065.2756829/150‑1 (MQ=60)
                                                                            GAAACCGGCAAGAGTCCCTCATACTGTCGGGGCTCCTGAGTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTT                                                                           <  SRR19973065.2993790/150‑1 (MQ=60)
                                                                            GAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTT                                                                           <  SRR19973065.684298/150‑1 (MQ=60)
                                                                            GAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTT                                                                           <  SRR19973065.684302/150‑1 (MQ=60)
                                                                                CCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGC                                                                       <  SRR19973065.2902769/150‑1 (MQ=60)
                                                                                CCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGC                                                                       <  SRR19973065.2901715/150‑1 (MQ=60)
                                                                                 CGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCG                                                                      <  SRR19973065.1862053/150‑1 (MQ=60)
                                                                                      AGAGTCCCTCAAAATGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATATCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAAGTATGGTTTTCATCTGCTTAAGATTTGCAATTTAAGCGCAGAA                                                                 >  SRR19973065.1632994/1‑150 (MQ=60)
                                                                                           CCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAA                                                            >  SRR19973065.2016052/1‑150 (MQ=60)
                                                                                            CCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAT                                                           >  SRR19973065.3109858/1‑150 (MQ=60)
                                                                                            CCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAT                                                           <  SRR19973065.3477794/150‑1 (MQ=60)
                                                                                            CCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAT                                                           >  SRR19973065.3294840/1‑150 (MQ=60)
                                                                                            CCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAT                                                           >  SRR19973065.3295898/1‑150 (MQ=60)
                                                                                            CCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAT                                                           >  SRR19973065.2828229/1‑150 (MQ=60)
                                                                                            CCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAT                                                           <  SRR19973065.1911369/150‑1 (MQ=60)
                                                                                             CTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATT                                                          >  SRR19973065.2889412/1‑150 (MQ=60)
                                                                                             CTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATT                                                          >  SRR19973065.279363/1‑150 (MQ=60)
                                                                                             CTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATT                                                          >  SRR19973065.2063443/1‑150 (MQ=60)
                                                                                              TCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTG                                                         <  SRR19973065.306866/150‑1 (MQ=60)
                                                                                              TCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTG                                                         >  SRR19973065.3071617/1‑150 (MQ=60)
                                                                                              TCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTG                                                         >  SRR19973065.3073687/1‑150 (MQ=60)
                                                                                              TCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTG                                                         >  SRR19973065.1956521/1‑150 (MQ=60)
                                                                                              TCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTG                                                         >  SRR19973065.1313292/1‑150 (MQ=60)
                                                                                              TCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTG                                                         <  SRR19973065.1263659/150‑1 (MQ=60)
                                                                                               CAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGC                                                        >  SRR19973065.709057/1‑150 (MQ=60)
                                                                                               CAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGC                                                        >  SRR19973065.708994/1‑150 (MQ=60)
                                                                                                   CTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAG                                                    <  SRR19973065.3289147/150‑1 (MQ=60)
                                                                                                   CTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAG                                                    <  SRR19973065.3532654/150‑1 (MQ=60)
                                                                                                    TGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGC                                                   <  SRR19973065.1313776/150‑1 (MQ=60)
                                                                                                    TGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGC                                                   <  SRR19973065.3258201/150‑1 (MQ=60)
                                                                                                     GTCGTGGCTCCTAATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATATGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCG                                                  >  SRR19973065.3030688/1‑150 (MQ=60)
                                                                                                        GTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGC                                               >  SRR19973065.3344983/1‑150 (MQ=60)
                                                                                                               CTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTT                                        >  SRR19973065.2645501/1‑150 (MQ=60)
                                                                                                                 GATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCATAAATCAATAGCGAAGCGTCCGCGGTTTCT                                      >  SRR19973065.1046433/1‑150 (MQ=60)
                                                                                                                 GATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCT                                      >  SRR19973065.1046429/1‑150 (MQ=60)
                                                                                                                       TGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAA                                >  SRR19973065.2300087/1‑150 (MQ=60)
                                                                                                                         GGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGT                              >  SRR19973065.2606837/1‑150 (MQ=60)
                                                                                                                           GCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGA                            <  SRR19973065.2169779/150‑1 (MQ=60)
                                                                                                                           GCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGA                            <  SRR19973065.2169553/150‑1 (MQ=60)
                                                                                                                             GATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAAT                          >  SRR19973065.3405910/1‑150 (MQ=60)
                                                                                                                              ATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATG                         >  SRR19973065.199223/1‑150 (MQ=60)
                                                                                                                                TTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTT                       <  SRR19973065.3468056/150‑1 (MQ=60)
                                                                                                                                TTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTT                       <  SRR19973065.3468102/150‑1 (MQ=60)
                                                                                                                                 TGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTT                      <  SRR19973065.3124261/150‑1 (MQ=60)
                                                                                                                                 TGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTT                      <  SRR19973065.3120921/150‑1 (MQ=60)
                                                                                                                                           AAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGG            <  SRR19973065.2616421/150‑1 (MQ=60)
                                                                                                                                            AAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGA           >  SRR19973065.3375908/1‑150 (MQ=60)
                                                                                                                                               CTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATG        <  SRR19973065.1849520/150‑1 (MQ=60)
                                                                                                                                                 TTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCC      <  SRR19973065.1888507/150‑1 (MQ=60)
                                                                                                                                                 TTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCC      <  SRR19973065.1892198/150‑1 (MQ=60)
                                                                                                                                                   ATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCG    >  SRR19973065.159989/1‑150 (MQ=60)
                                                                                                                                                   ATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCG    >  SRR19973065.160126/1‑150 (MQ=60)
                                                                                                                                                     AGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTC  >  SRR19973065.3471456/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTC  >  CP009273/231229‑231527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑