Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 337,720 G→T 100% E295D (GAG→GAT)  yahJ → putative metallo‑dependent hydrolase domain deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273337,7200GT96.1% 478.3 / 7.5 165E295D (GAG→GAT) yahJputative metallo‑dependent hydrolase domain deaminase
Reads supporting (aligned to +/- strand):  ref base G (1/6);  new base T (80/78);  total (81/84)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

CGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTG  >  CP009273/337575‑337864
                                                                                                                                                 |                                                                                                                                                
cGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaa                                                                                                                                                >  1:700922/1‑148 (MQ=255)
 gACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaaa                                                                                                                                               <  1:2645384/148‑1 (MQ=255)
 gACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaaa                                                                                                                                               <  1:2646458/148‑1 (MQ=255)
  aCCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaaaa                                                                                                                                              >  1:3525782/1‑148 (MQ=255)
    cAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACg                                                                                                                                            <  2:804274/148‑1 (MQ=255)
      gtgtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGcc                                                                                                                                          <  2:208944/148‑1 (MQ=255)
      gtgtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGAGATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGcc                                                                                                                                          <  2:2651857/148‑1 (MQ=255)
       tgtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCca                                                                                                                                         >  2:1636273/1‑148 (MQ=255)
          tGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAg                                                                                                                                      >  2:1407459/1‑148 (MQ=255)
            aTGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCt                                                                                                                                    <  2:1816255/148‑1 (MQ=255)
            aTGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCt                                                                                                                                    >  2:1856394/1‑148 (MQ=255)
             tGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTg                                                                                                                                   <  2:853070/148‑1 (MQ=255)
              ggCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGa                                                                                                                                  <  1:461379/148‑1 (MQ=255)
               gcgcGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAACGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGaa                                                                                                                                 <  2:1495536/148‑1 (MQ=255)
               gcgcGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACTAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGaa                                                                                                                                 <  1:2914500/148‑1 (MQ=255)
               gcgcGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGaa                                                                                                                                 <  2:1495527/148‑1 (MQ=255)
                 gcgATGTAAAAATCCCTCGACACCTTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAgg                                                                                                                               <  1:1896055/148‑1 (MQ=255)
                 gcgATGGAAAAAGCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATAAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAgg                                                                                                                               <  1:1898349/148‑1 (MQ=255)
                    aTGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCa                                                                                                                            >  1:130448/1‑148 (MQ=255)
                      ggAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCCCCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAg                                                                                                                          <  1:1115910/148‑1 (MQ=255)
                      ggAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAg                                                                                                                          >  1:3388704/1‑148 (MQ=255)
                      ggAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAg                                                                                                                          <  2:1142896/148‑1 (MQ=255)
                          aaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGa                                                                                                                      >  2:460718/1‑148 (MQ=255)
                           aaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGAc                                                                                                                     >  2:753913/1‑148 (MQ=255)
                                cTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCa                                                                                                                >  2:3340235/1‑148 (MQ=255)
                                cTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCa                                                                                                                >  1:2743259/1‑148 (MQ=255)
                                cTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCa                                                                                                                >  2:1571607/1‑148 (MQ=255)
                                  cGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGt                                                                                                              <  1:2154434/148‑1 (MQ=255)
                                  cGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGt                                                                                                              >  1:1758643/1‑148 (MQ=255)
                                  cGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGt                                                                                                              >  1:2957556/1‑148 (MQ=255)
                                     cacCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCAc                                                                                                           >  2:1706496/1‑148 (MQ=255)
                                      acCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATCTGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACg                                                                                                          >  2:3483658/1‑148 (MQ=255)
                                       ccATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTGGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGc                                                                                                         >  1:25537/1‑148 (MQ=255)
                                         aTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCt                                                                                                       >  1:977989/1‑148 (MQ=255)
                                         aTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCt                                                                                                       <  1:1891711/148‑1 (MQ=255)
                                         aTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCt                                                                                                       <  2:1482362/148‑1 (MQ=255)
                                          tGTTCCAGATTGCGCTGGACTACGACACAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCtt                                                                                                      <  2:2377974/148‑1 (MQ=255)
                                          tGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCtt                                                                                                      <  2:1924762/148‑1 (MQ=255)
                                          tGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCtt                                                                                                      <  1:2337051/148‑1 (MQ=255)
                                            ttCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTg                                                                                                    >  1:1787843/1‑148 (MQ=255)
                                               cAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAACACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTgcgc                                                                                                 <  1:862909/148‑1 (MQ=255)
                                               cAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTgcgc                                                                                                 <  2:432275/148‑1 (MQ=255)
                                               cAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTgcgc                                                                                                 <  1:761677/148‑1 (MQ=255)
                                                        cTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGc                                                                                        >  1:303170/1‑148 (MQ=255)
                                                           gACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCa                                                                                     <  2:700922/148‑1 (MQ=255)
                                                           gACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCa                                                                                     >  2:3062275/1‑148 (MQ=255)
                                                           gACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCa                                                                                     <  1:1317846/148‑1 (MQ=255)
                                                              tACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACg                                                                                  >  2:2967221/1‑148 (MQ=255)
                                                              tACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACg                                                                                  >  2:2968465/1‑148 (MQ=255)
                                                               aCGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGa                                                                                 >  1:2236069/1‑148 (MQ=255)
                                                                 gACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGc                                                                               <  1:481033/148‑1 (MQ=255)
                                                                  aCAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCa                                                                              >  1:1615078/1‑148 (MQ=255)
                                                                     aaGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACa                                                                           >  1:3296492/1‑148 (MQ=255)
                                                                      aGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAg                                                                          <  1:2252849/148‑1 (MQ=255)
                                                                      aGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAg                                                                          <  2:2482587/148‑1 (MQ=255)
                                                                       ggCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAgg                                                                         >  2:1772832/1‑148 (MQ=255)
                                                                         cGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTa                                                                       >  2:2703618/1‑148 (MQ=255)
                                                                          gTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAg                                                                      <  1:1024584/148‑1 (MQ=255)
                                                                           tCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGa                                                                     >  2:616444/1‑148 (MQ=255)
                                                                           tCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGa                                                                     <  2:3533367/148‑1 (MQ=255)
                                                                             gATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATg                                                                   <  2:3234632/148‑1 (MQ=255)
                                                                              atatTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTTCGCTGGCAACGCTCAACGAGCAACAGGTAGATGa                                                                  <  2:57698/148‑1 (MQ=255)
                                                                                      cTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGa                                                          <  1:2026871/148‑1 (MQ=255)
                                                                                       tGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGaa                                                         <  2:224167/148‑1 (MQ=255)
                                                                                          aCGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCg                                                      >  1:435469/1‑148 (MQ=255)
                                                                                           cGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCgg                                                     >  2:238292/1‑148 (MQ=255)
                                                                                           cGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCgg                                                     >  2:2164907/1‑148 (MQ=255)
                                                                                              aaCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtg                                                  >  2:1935987/1‑148 (MQ=255)
                                                                                                ccACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtggt                                                >  1:2867130/1‑148 (MQ=255)
                                                                                                    tCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGgtggtg                                            <  1:3419300/148‑1 (MQ=255)
                                                                                                       ggCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaa                                         <  1:52733/148‑1 (MQ=255)
                                                                                                         cAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaaca                                       >  1:192048/1‑148 (MQ=255)
                                                                                                           ggCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCGGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACaaa                                     <  2:2102513/148‑1 (MQ=255)
                                                                                                            gCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAt                                    >  2:2335493/1‑148 (MQ=255)
                                                                                                            gCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAt                                    >  1:2364827/1‑148 (MQ=255)
                                                                                                            gCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAt                                    >  1:2090177/1‑148 (MQ=255)
                                                                                                               tGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTc                                 >  2:161248/1‑148 (MQ=255)
                                                                                                                ggCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCt                                <  2:2816395/148‑1 (MQ=255)
                                                                                                                 gCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTa                               >  2:1321884/1‑148 (MQ=255)
                                                                                                                     ccATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGc                           >  2:2658633/1‑148 (MQ=255)
                                                                                                                     ccATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGc                           <  1:2173866/148‑1 (MQ=255)
                                                                                                                     ccATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGc                           >  2:3274068/1‑148 (MQ=255)
                                                                                                                     acataatttATATGGTTTAAACTGTAGATAAAACGACACAGCTGAAGGGAAATCTGACAATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGATCATCAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGc                           <  1:2173592/141‑1 (MQ=255)
                                                                                                                      cATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGcc                          >  2:1147705/1‑148 (MQ=255)
                                                                                                                      cATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGcc                          >  2:1084809/1‑148 (MQ=255)
                                                                                                                      cATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGcc                          >  2:2864342/1‑148 (MQ=255)
                                                                                                                         cAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                       >  1:1086177/1‑148 (MQ=255)
                                                                                                                         cAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                       >  1:3125996/1‑148 (MQ=255)
                                                                                                                         cAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                       >  1:1743001/1‑148 (MQ=255)
                                                                                                                             tataTGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACgg                   >  2:3212414/1‑148 (MQ=255)
                                                                                                                               tatGTTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTg                 <  1:1408444/148‑1 (MQ=255)
                                                                                                                               tatGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTg                 >  2:945373/1‑148 (MQ=255)
                                                                                                                               tatGGTTGAAAATGTAGATAATACGCAACAGCTGAAGGGCAAGCTGACCATCAGTCTCGCCTTTGCGCTGTCAACTCTCAAAGAGCAACAGGTAGATGAAATGGCGAACCGGATGGTTGTTCAACATATTTCTATCGCCTCGACGGTg                 <  2:2179101/148‑1 (MQ=255)
                                                                                                                                    ttGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAt            >  1:420279/1‑148 (MQ=255)
                                                                                                                                     tGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAtt           <  2:472525/148‑1 (MQ=255)
                                                                                                                                     tGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAtt           >  1:3088240/1‑148 (MQ=255)
                                                                                                                                     tGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAtt           <  1:1856241/148‑1 (MQ=255)
                                                                                                                                        aaCGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGc        >  1:1618739/1‑148 (MQ=255)
                                                                                                                                             tAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCt   >  2:3155759/1‑148 (MQ=255)
                                                                                                                                              agatAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTg  <  2:2024502/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTG  >  CP009273/337575‑337864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCA  >  CP009273/337573‑337866
                                                                                                                                                   |                                                                                                                                                  
CCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAA                                                                                                                                                  >  SRR19973066.706095/1‑150 (MQ=60)
  CGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAA                                                                                                                                                >  SRR19973066.3549248/1‑150 (MQ=60)
   GACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAAC                                                                                                                                               <  SRR19973066.2664236/150‑1 (MQ=60)
   GACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAAC                                                                                                                                               <  SRR19973066.2663172/150‑1 (MQ=60)
   GACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAAC                                                                                                                                               <  SRR19973066.2663159/150‑1 (MQ=60)
        GTTTTGATGGCGCGATGTAAAAATCCCTCGACACCTTGTTCCAGATTGCGCTGGACTACGACAACGGGGTCTATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCAATCAATTATATGGTTGAAACGGTAGATAAAACGCCAC                                                                                                                                          <  SRR19973066.1864225/150‑1 (MQ=60)
          GTTGTTGGCGCGTTGTAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCTATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCAATAAATTATATGGTTGAAACGGTAGATAAAACGCCACAG                                                                                                                                        <  SRR19973066.362573/150‑1 (MQ=60)
            TGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGAATACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCT                                                                                                                                      <  SRR19973066.3172650/150‑1 (MQ=60)
             GATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGTTTGCGCTGGACTACGACAAAGGCGTCTATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTG                                                                                                                                     <  SRR19973066.1927778/150‑1 (MQ=60)
                GGCGCTATTGAAAAATCCCTCGACACCATGTTCCATATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAG                                                                                                                                  <  SRR19973066.2270914/150‑1 (MQ=60)
                GGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAG                                                                                                                                  <  SRR19973066.464909/150‑1 (MQ=60)
                 GCGCGATGTAAAAAACCCTCGACCCAATGTTCCAGATTGCGCTGGACTACGACAATGGCGTCTATTTTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCAACATTTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGG                                                                                                                                 <  SRR19973066.3039890/150‑1 (MQ=60)
                 GCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACTAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGG                                                                                                                                 <  SRR19973066.2933944/150‑1 (MQ=60)
                 GCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGG                                                                                                                                 <  SRR19973066.2933779/150‑1 (MQ=60)
                  CGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGG                                                                                                                                >  SRR19973066.3392420/1‑150 (MQ=60)
                   GCGATGTAAAAATCCCTCGACACCTTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGC                                                                                                                               <  SRR19973066.1909577/150‑1 (MQ=60)
                   GCGATGGAAAAAGCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATAAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGC                                                                                                                               <  SRR19973066.1911892/150‑1 (MQ=60)
                    CGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCA                                                                                                                              >  SRR19973066.131729/1‑150 (MQ=60)
                    CGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCA                                                                                                                              >  SRR19973066.476107/1‑150 (MQ=60)
                      ATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAG                                                                                                                            >  SRR19973066.3411319/1‑150 (MQ=60)
                       TGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGC                                                                                                                           >  SRR19973066.1707274/1‑150 (MQ=60)
                        GGAAAAATCCCTCGACACCAGGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCCCCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCT                                                                                                                          <  SRR19973066.1123965/150‑1 (MQ=60)
                         GAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAATCTG                                                                                                                         >  SRR19973066.3446761/1‑150 (MQ=60)
                                CCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCA                                                                                                                  >  SRR19973066.2761716/1‑150 (MQ=60)
                                  CTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGT                                                                                                                >  SRR19973066.1771274/1‑150 (MQ=60)
                                  CTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGT                                                                                                                >  SRR19973066.2977154/1‑150 (MQ=60)
                                    CGACACCATGTTCCAGATTGCGCTGTACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCA                                                                                                              <  SRR19973066.2169394/150‑1 (MQ=60)
                                    CGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCA                                                                                                              <  SRR19973066.2822002/150‑1 (MQ=60)
                                       CACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTGGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGC                                                                                                           >  SRR19973066.25903/1‑150 (MQ=60)
                                       CACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGC                                                                                                           >  SRR19973066.25913/1‑150 (MQ=60)
                                         CCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCT                                                                                                         >  SRR19973066.985197/1‑150 (MQ=60)
                                          CATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTT                                                                                                        >  SRR19973066.548672/1‑150 (MQ=60)
                                           ATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTT                                                                                                       <  SRR19973066.1905198/150‑1 (MQ=60)
                                            TGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTG                                                                                                      >  SRR19973066.1800589/1‑150 (MQ=60)
                                            TGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTG                                                                                                      <  SRR19973066.2353611/150‑1 (MQ=60)
                                            TGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTG                                                                                                      <  SRR19973066.2352976/150‑1 (MQ=60)
                                              TTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCG                                                                                                    <  SRR19973066.1102990/150‑1 (MQ=60)
                                                 CAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAACACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTG                                                                                                 <  SRR19973066.869116/150‑1 (MQ=60)
                                                 CAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTG                                                                                                 <  SRR19973066.767099/150‑1 (MQ=60)
                                                 CAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTG                                                                                                 <  SRR19973066.767324/150‑1 (MQ=60)
                                                        CGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGC                                                                                          >  SRR19973066.305327/1‑150 (MQ=60)
                                                           TGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCA                                                                                       <  SRR19973066.2239907/150‑1 (MQ=60)
                                                             GACTACGACAACGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAAC                                                                                     <  SRR19973066.1327395/150‑1 (MQ=60)
                                                               CTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGA                                                                                   >  SRR19973066.2251494/1‑150 (MQ=60)
                                                                  CGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCA                                                                                >  SRR19973066.1626796/1‑150 (MQ=60)
                                                                   GACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAA                                                                               <  SRR19973066.484653/150‑1 (MQ=60)
                                                                     CAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACA                                                                             >  SRR19973066.3318267/1‑150 (MQ=60)
                                                                      AAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCTACGCTCAACGAGCAACAG                                                                            >  SRR19973066.1503305/1‑150 (MQ=60)
                                                                        AGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGT                                                                          <  SRR19973066.2268381/150‑1 (MQ=60)
                                                                            GTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGAT                                                                      <  SRR19973066.1032125/150‑1 (MQ=60)
                                                                                    TCACCGGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGGTGAACTGGC                                                              <  SRR19973066.1301825/150‑1 (MQ=60)
                                                                                        CTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAAC                                                          <  SRR19973066.2041397/150‑1 (MQ=60)
                                                                                        CTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAAC                                                          <  SRR19973066.2041037/150‑1 (MQ=60)
                                                                                          GCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCG                                                        >  SRR19973066.438843/1‑150 (MQ=60)
                                                                                             CGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAT                                                     <  SRR19973066.1910553/150‑1 (MQ=60)
                                                                                                AACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGT                                                  >  SRR19973066.2886364/1‑150 (MQ=60)
                                                                                                      TCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCA                                            <  SRR19973066.3442173/150‑1 (MQ=60)
                                                                                                         GGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACA                                         <  SRR19973066.53402/150‑1 (MQ=60)
                                                                                                         GGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACA                                         >  SRR19973066.193701/1‑150 (MQ=60)
                                                                                                            AGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAT                                      >  SRR19973066.2104581/1‑150 (MQ=60)
                                                                                                            AGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAT                                      >  SRR19973066.2380840/1‑150 (MQ=60)
                                                                                                                  GGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTAT                                <  SRR19973066.1417576/150‑1 (MQ=60)
                                                                                                                    gagaCATCATTTATATGGTTGAAACGGTAGATAAAACGCCTCAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACTCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCG                              <  SRR19973066.2293779/146‑1 (MQ=60)
                                                                                                                    CAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCG                              >  SRR19973066.3071558/1‑150 (MQ=60)
                                                                                                                    CAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCG                              >  SRR19973066.448736/1‑150 (MQ=60)
                                                                                                                       acataatTTATATGGTTTAAACTGTAGATAAAACGACACAGCTGAAGGGAAATCTGACAATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGATCATCAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCT                           <  SRR19973066.2188691/143‑1 (MQ=60)
                                                                                                                       CCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCT                           <  SRR19973066.2188695/150‑1 (MQ=60)
                                                                                                                       CCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCT                           <  SRR19973066.2188967/150‑1 (MQ=60)
                                                                                                                         ATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCG                         >  SRR19973066.3146868/1‑150 (MQ=60)
                                                                                                                         ATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCG                         >  SRR19973066.1094101/1‑150 (MQ=60)
                                                                                                                         ATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCG                         >  SRR19973066.1755559/1‑150 (MQ=60)
                                                                                                                               TATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTG                   <  SRR19973066.2159850/150‑1 (MQ=60)
                                                                                                                               TATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTG                   <  SRR19973066.1006669/150‑1 (MQ=60)
                                                                                                                                 TATGTTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCC                 <  SRR19973066.1418566/150‑1 (MQ=60)
                                                                                                                                    GGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAT              >  SRR19973066.423550/1‑150 (MQ=60)
                                                                                                                                    GGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAT              <  SRR19973066.1762851/150‑1 (MQ=60)
                                                                                                                                     GTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATT             >  SRR19973066.3108870/1‑150 (MQ=60)
                                                                                                                                       TGAAACGGTATATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGG           <  SRR19973066.1869673/150‑1 (MQ=60)
                                                                                                                                       TGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGG           <  SRR19973066.1869520/150‑1 (MQ=60)
                                                                                                                                        GAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGC          >  SRR19973066.1630474/1‑150 (MQ=60)
                                                                                                                                                AGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCA  >  SRR19973066.124063/1‑150 (MQ=60)
                                                                                                                                                AGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCA  >  SRR19973066.124032/1‑150 (MQ=60)
                                                                                                                                                AGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCA  <  SRR19973066.952272/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
CCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCA  >  CP009273/337573‑337866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑