Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 337,720 G→T 100% E295D (GAG→GAT)  yahJ → putative metallo‑dependent hydrolase domain deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273337,7200GT100.0% 550.5 / NA 171E295D (GAG→GAT) yahJputative metallo‑dependent hydrolase domain deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (97/74);  total (97/74)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

AGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTG  >  CP009273/337580‑337864
                                                                                                                                            |                                                                                                                                                
aGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGc                                                                                                                                           >  2:2376088/1‑148 (MQ=255)
 gtgtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGcc                                                                                                                                          >  2:2791388/1‑148 (MQ=255)
   gtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCcac                                                                                                                                        >  2:1570314/1‑148 (MQ=255)
    ttGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCcaca                                                                                                                                       >  2:412972/1‑148 (MQ=255)
    ttGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCcaca                                                                                                                                       >  1:3465522/1‑148 (MQ=255)
     tGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAg                                                                                                                                      <  2:2119882/148‑1 (MQ=255)
          gcgcGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGAGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTCaa                                                                                                                                 >  1:1866021/1‑148 (MQ=255)
             cgATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAggg                                                                                                                              >  2:795547/1‑148 (MQ=255)
                tGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATACAACGCCACAGCTGAAGGGCaa                                                                                                                           >  2:567539/1‑148 (MQ=255)
                tGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGTGCaa                                                                                                                           >  2:567549/1‑148 (MQ=255)
                tGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCaa                                                                                                                           >  2:1005348/1‑148 (MQ=255)
                 ggAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAg                                                                                                                          >  1:1485779/1‑148 (MQ=255)
                 ggAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAg                                                                                                                          <  1:2342050/148‑1 (MQ=255)
                 ggAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAg                                                                                                                          <  2:1272853/148‑1 (MQ=255)
                  gAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGc                                                                                                                         >  1:556693/1‑148 (MQ=255)
                   aaaaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCt                                                                                                                        >  2:16471/1‑148 (MQ=255)
                     aaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGa                                                                                                                      <  2:2473585/148‑1 (MQ=255)
                       aTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGAcc                                                                                                                    >  2:2325747/1‑148 (MQ=255)
                        tCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCa                                                                                                                   >  1:3003841/1‑148 (MQ=255)
                         cccTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCAt                                                                                                                  >  1:3347070/1‑148 (MQ=255)
                                   cATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGcc                                                                                                        >  1:2198531/1‑148 (MQ=255)
                                     tGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGGGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCtt                                                                                                      <  2:603590/148‑1 (MQ=255)
                                       ttCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTg                                                                                                    >  1:2848134/1‑148 (MQ=255)
                                             aTTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTgg                                                                                              >  1:2936155/1‑148 (MQ=255)
                                                gcgcTGGACTACGACAAAGGCTTCGATATTCACCGGCACGAAACCACTCCGGCAGGAGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATAAGTCACGCCTTTGCGCTGGCaa                                                                                           <  2:3310684/148‑1 (MQ=255)
                                                     ggACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTc                                                                                      >  1:3280517/1‑148 (MQ=255)
                                                     ggACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAAATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTc                                                                                      >  2:481922/1‑148 (MQ=255)
                                                     ggACTACGAAAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGCCAAGCTGACCATCACTCACGCCTTTGCGCTGGCAACGCTc                                                                                      >  1:3280583/1‑148 (MQ=255)
                                                       aCTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCaa                                                                                    <  2:390741/148‑1 (MQ=255)
                                                          aCGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGa                                                                                 >  1:3162139/1‑148 (MQ=255)
                                                          aCGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGATGGCAACGCTCAACGa                                                                                 >  2:1325081/1‑148 (MQ=255)
                                                           cGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAg                                                                                >  1:1104844/1‑148 (MQ=255)
                                                            gACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGc                                                                               <  2:2568515/148‑1 (MQ=255)
                                                                aaGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACa                                                                           <  2:949723/148‑1 (MQ=255)
                                                                   gCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGt                                                                        <  2:1340571/148‑1 (MQ=255)
                                                                       cGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGAt                                                                    <  1:2628774/148‑1 (MQ=255)
                                                                       cGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGAt                                                                    >  2:735508/1‑148 (MQ=255)
                                                                           atTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAAc                                                                >  2:1294813/1‑148 (MQ=255)
                                                                             tCACCTGCACGAAACCGCTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTg                                                              <  2:364946/148‑1 (MQ=255)
                                                                             tCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTg                                                              >  2:1770035/1‑148 (MQ=255)
                                                                             tCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTg                                                              >  2:1125585/1‑148 (MQ=255)
                                                                               aCCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGc                                                            <  2:2453464/148‑1 (MQ=255)
                                                                               aCCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGc                                                            >  2:2198397/1‑148 (MQ=255)
                                                                                ccTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCg                                                           >  2:2516437/1‑148 (MQ=255)
                                                                                  tGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGaa                                                         <  2:827969/148‑1 (MQ=255)
                                                                                   gCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAAc                                                        >  2:3405172/1‑148 (MQ=255)
                                                                                     aCGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCg                                                      <  1:1087857/148‑1 (MQ=255)
                                                                                     aCGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCg                                                      <  1:1087850/148‑1 (MQ=255)
                                                                                      cGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCgg                                                     >  1:2454562/1‑148 (MQ=255)
                                                                                       gAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGa                                                    <  2:2520487/148‑1 (MQ=255)
                                                                                        aaaCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAt                                                   >  1:702591/1‑148 (MQ=255)
                                                                                         aaCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtg                                                  >  1:1714299/1‑148 (MQ=255)
                                                                                         aaCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtg                                                  <  2:3005095/148‑1 (MQ=255)
                                                                                         aaCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtg                                                  <  2:3004849/148‑1 (MQ=255)
                                                                                          aCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACTGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtgg                                                 >  1:875896/1‑148 (MQ=255)
                                                                                          aCCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtgg                                                 >  2:2679711/1‑148 (MQ=255)
                                                                                            cACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCACCAGGTAGATGAACTGGCGAACCGGAtggtg                                               >  2:2740910/1‑148 (MQ=255)
                                                                                            cACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtggtg                                               <  1:826283/148‑1 (MQ=255)
                                                                                               tCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGgtggtg                                            <  1:16471/148‑1 (MQ=255)
                                                                                                ccGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGc                                           >  2:1713582/1‑148 (MQ=255)
                                                                                                 cGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGca                                          <  1:1114607/148‑1 (MQ=255)
                                                                                                 cGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGca                                          <  1:1114616/148‑1 (MQ=255)
                                                                                                 cGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGca                                          <  1:1114630/148‑1 (MQ=255)
                                                                                                 cGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGca                                          >  2:2989959/1‑148 (MQ=255)
                                                                                                   gCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaac                                        >  1:1898562/1‑148 (MQ=255)
                                                                                                     aGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaacaa                                      <  2:1418647/148‑1 (MQ=255)
                                                                                                     aGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaacaa                                      <  1:1376083/148‑1 (MQ=255)
                                                                                                      ggCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACaaa                                     >  2:1896389/1‑148 (MQ=255)
                                                                                                         gTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAttt                                  <  2:1544565/148‑1 (MQ=255)
                                                                                                           ggCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCt                                <  1:1218112/148‑1 (MQ=255)
                                                                                                            gCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTa                               >  2:2078363/1‑148 (MQ=255)
                                                                                                             cAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTAt                              <  2:2197779/148‑1 (MQ=255)
                                                                                                             cAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTAt                              <  2:2197699/148‑1 (MQ=255)
                                                                                                               gCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCg                            >  2:2084997/1‑148 (MQ=255)
                                                                                                               gCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCg                            >  1:2140538/1‑148 (MQ=255)
                                                                                                                 cATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGcc                          >  2:1264642/1‑148 (MQ=255)
                                                                                                                 cATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGcc                          <  2:627363/148‑1 (MQ=255)
                                                                                                                 cATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGcc                          <  2:1485779/148‑1 (MQ=255)
                                                                                                                  aTCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCt                         <  1:2189407/148‑1 (MQ=255)
                                                                                                                    cAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                       <  2:1084454/148‑1 (MQ=255)
                                                                                                                    cAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                       >  2:2703360/1‑148 (MQ=255)
                                                                                                                    cAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                       <  2:1084580/148‑1 (MQ=255)
                                                                                                                    cAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                       <  2:3352535/148‑1 (MQ=255)
                                                                                                                       ttATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACTCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACg                    >  1:2034234/1‑148 (MQ=255)
                                                                                                                       ttATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACg                    <  2:1248507/148‑1 (MQ=255)
                                                                                                                       ttATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACg                    >  1:2192126/1‑148 (MQ=255)
                                                                                                                          tatGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTg                 <  2:878077/148‑1 (MQ=255)
                                                                                                                          tatGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTg                 >  1:2912313/1‑148 (MQ=255)
                                                                                                                              gTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGa             <  2:519028/148‑1 (MQ=255)
                                                                                                                              gTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGa             <  2:519709/148‑1 (MQ=255)
                                                                                                                               ttGAAACGGTATATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAt            <  1:1570314/148‑1 (MQ=255)
                                                                                                                                   aaCGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGc        <  2:3465522/148‑1 (MQ=255)
                                                                                                                                    aCGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCa       >  1:993211/1‑148 (MQ=255)
                                                                                                                                    aCGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCa       >  2:2583095/1‑148 (MQ=255)
                                                                                                                                    aCGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCa       <  2:2292488/148‑1 (MQ=255)
                                                                                                                                    aCGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCa       >  2:2324419/1‑148 (MQ=255)
                                                                                                                                     cGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCAc      >  1:482797/1‑148 (MQ=255)
                                                                                                                                      ggTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACg     <  2:429723/148‑1 (MQ=255)
                                                                                                                                        tAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCt   >  1:1910633/1‑148 (MQ=255)
                                                                                                                                        tAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCt   >  2:1712716/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                
AGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTG  >  CP009273/337580‑337864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCATA  >  CP009273/337571‑337868
                                                                                                                                                     |                                                                                                                                                    
GACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTCTATGGTTGAAACGGTAGAT                                                                                                                                                      >  SRR19973065.3329610/1‑150 (MQ=60)
GACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAT                                                                                                                                                      >  SRR19973065.3452273/1‑150 (MQ=60)
GACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAT                                                                                                                                                      >  SRR19973065.3329587/1‑150 (MQ=60)
 ACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATA                                                                                                                                                     <  SRR19973065.1003053/150‑1 (MQ=60)
 ACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATA                                                                                                                                                     <  SRR19973065.1003048/150‑1 (MQ=60)
           TGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACA                                                                                                                                           >  SRR19973065.3486627/1‑150 (MQ=60)
               GATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGTTTGCGCTGGACTACGACAAAGGCGTCTATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTG                                                                                                                                       <  SRR19973065.3110149/150‑1 (MQ=60)
                 TGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGAGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTCAA                                                                                                                                     >  SRR19973065.1878301/1‑150 (MQ=60)
                        ATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAG                                                                                                                              >  SRR19973065.1495260/1‑150 (MQ=60)
                         TGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGC                                                                                                                             >  SRR19973065.2335922/1‑150 (MQ=60)
                         TGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGC                                                                                                                             >  SRR19973065.631448/1‑150 (MQ=60)
                         TGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGC                                                                                                                             >  SRR19973065.560247/1‑150 (MQ=60)
                          GGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCT                                                                                                                            <  SRR19973065.2356668/150‑1 (MQ=60)
                             AAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGAC                                                                                                                         >  SRR19973065.3223782/1‑150 (MQ=60)
                               AATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCA                                                                                                                       >  SRR19973065.3022244/1‑150 (MQ=60)
                                ATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCAT                                                                                                                      >  SRR19973065.3367519/1‑150 (MQ=60)
                                   CCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAG                                                                                                                   >  SRR19973065.1521221/1‑150 (MQ=60)
                                    CTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGT                                                                                                                  <  SRR19973065.3406234/150‑1 (MQ=60)
                                        ACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACG                                                                                                              >  SRR19973065.2951455/1‑150 (MQ=60)
                                          ACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCC                                                                                                            >  SRR19973065.2212609/1‑150 (MQ=60)
                                              TGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTG                                                                                                        >  SRR19973065.2865656/1‑150 (MQ=60)
                                                    AGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGG                                                                                                  >  SRR19973065.2953961/1‑150 (MQ=60)
                                                     GATTGCGCTGGACTACGACAAAGGCGTCTATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATTTTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGC                                                                                                 <  SRR19973065.2200101/150‑1 (MQ=60)
                                                            CTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTC                                                                                          >  SRR19973065.3300456/1‑150 (MQ=60)
                                                            CTGGACTACGAAAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGCCAAGCTGACCATCACTCACGCCTTTGCGCTGGCAACGCTC                                                                                          >  SRR19973065.3300523/1‑150 (MQ=60)
                                                                 CTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGA                                                                                     >  SRR19973065.3181293/1‑150 (MQ=60)
                                                                  TACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAG                                                                                    >  SRR19973065.1111641/1‑150 (MQ=60)
                                                                  TACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAG                                                                                    >  SRR19973065.1112089/1‑150 (MQ=60)
                                                                          AGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGT                                                                            >  SRR19973065.469652/1‑150 (MQ=60)
                                                                                CGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGA                                                                      <  SRR19973065.2645034/150‑1 (MQ=60)
                                                                                CGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGA                                                                      <  SRR19973065.1587616/150‑1 (MQ=60)
                                                                                     TTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGG                                                                 >  SRR19973065.3310289/1‑150 (MQ=60)
                                                                                     TTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGG                                                                 >  SRR19973065.2139913/1‑150 (MQ=60)
                                                                                          CTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAAC                                                            >  SRR19973065.772663/1‑150 (MQ=60)
                                                                                             CACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGG                                                         >  SRR19973065.2470031/1‑150 (MQ=60)
                                                                                              ACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGA                                                        <  SRR19973065.1095022/150‑1 (MQ=60)
                                                                                              ACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGA                                                        <  SRR19973065.1095029/150‑1 (MQ=60)
                                                                                               CGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAT                                                       >  SRR19973065.707262/1‑150 (MQ=60)
                                                                                                GAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATG                                                      >  SRR19973065.1725694/1‑150 (MQ=60)
                                                                                                 AAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACTGTAGATAAAACGCCACAGCTGAAGGGCAAGATGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGTCGAACCGGATGG                                                     >  SRR19973065.881490/1‑150 (MQ=60)
                                                                                                   ACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTG                                                   <  SRR19973065.2368070/150‑1 (MQ=60)
                                                                                                     CACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGT                                                 <  SRR19973065.831695/150‑1 (MQ=60)
                                                                                                        TCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCA                                              <  SRR19973065.16711/150‑1 (MQ=60)
                                                                                                          CGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAAC                                            <  SRR19973065.1121910/150‑1 (MQ=60)
                                                                                                          CGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAAC                                            >  SRR19973065.2916207/1‑150 (MQ=60)
                                                                                                          CGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAAC                                            <  SRR19973065.1121908/150‑1 (MQ=60)
                                                                                                          CGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAAC                                            >  SRR19973065.1911038/1‑150 (MQ=60)
                                                                                                          CGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAAC                                            <  SRR19973065.1121896/150‑1 (MQ=60)
                                                                                                          CGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAAC                                            <  SRR19973065.1121887/150‑1 (MQ=60)
                                                                                                          CGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAAC                                            <  SRR19973065.1121886/150‑1 (MQ=60)
                                                                                                              AGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAACACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAT                                        <  SRR19973065.1385159/150‑1 (MQ=60)
                                                                                                              AGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAAT                                        >  SRR19973065.1784504/1‑150 (MQ=60)
                                                                                                                    GGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTAT                                  <  SRR19973065.1226021/150‑1 (MQ=60)
                                                                                                                    GGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTAT                                  <  SRR19973065.1226026/150‑1 (MQ=60)
                                                                                                                      CAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCG                                >  SRR19973065.2154305/1‑150 (MQ=60)
                                                                                                                           ATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCG                           <  SRR19973065.2203448/150‑1 (MQ=60)
                                                                                                                              AATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACTCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCTAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACG                        >  SRR19973065.2047280/1‑150 (MQ=60)
                                                                                                                              AATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACG                        >  SRR19973065.2206182/1‑150 (MQ=60)
                                                                                                                              AATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACG                        >  SRR19973065.2206228/1‑150 (MQ=60)
                                                                                                                                 TATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTG                     >  SRR19973065.2930036/1‑150 (MQ=60)
                                                                                                                                     TGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTATATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGA                 <  SRR19973065.2808691/150‑1 (MQ=60)
                                                                                                                                        TTGAAACGGTATATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTG              <  SRR19973065.1580758/150‑1 (MQ=60)
                                                                                                                                        TTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTG              <  SRR19973065.1893779/150‑1 (MQ=60)
                                                                                                                                           AAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCA           >  SRR19973065.999737/1‑150 (MQ=60)
                                                                                                                                           AAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCA           >  SRR19973065.999812/1‑150 (MQ=60)
                                                                                                                                            AACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCAC          >  SRR19973065.486080/1‑150 (MQ=60)
                                                                                                                                               GGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCT       >  SRR19973065.1923181/1‑150 (MQ=60)
                                                                                                                                               GGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCT       >  SRR19973065.1922873/1‑150 (MQ=60)
                                                                                                                                               GGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCT       >  SRR19973065.1148930/1‑150 (MQ=60)
                                                                                                                                                  AGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGCTTGGCACGCTGCA    >  SRR19973065.2279051/1‑150 (MQ=60)
                                                                                                                                                   GATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCAT   <  SRR19973065.2314699/150‑1 (MQ=60)
                                                                                                                                                   GATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCAT   <  SRR19973065.1905771/150‑1 (MQ=60)
                                                                                                                                                   GATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCAT   <  SRR19973065.1904759/150‑1 (MQ=60)
                                                                                                                                                   GATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCAT   <  SRR19973065.1904718/150‑1 (MQ=60)
                                                                                                                                                    ATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCATA  <  SRR19973065.3408758/150‑1 (MQ=60)
                                                                                                                                                    ATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCATA  <  SRR19973065.3408504/150‑1 (MQ=60)
                                                                                                                                                    ATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCATA  <  SRR19973065.2488380/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCATA  >  CP009273/337571‑337868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑