Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 706,097 A→C 100% T2T (ACT→ACG) 
*29G (TGA→GGA) 		 fur ←
uof ←		 ferric iron uptake regulon transcriptional repressor; autorepressor
ryhB‑regulated fur leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273706,0970AC96.2% 905.0 / 9.1 235T2T (ACT→ACG) 
*29G (TGA→GGA) 		fur
uof		ferric iron uptake regulon transcriptional repressor; autorepressor
ryhB‑regulated fur leader peptide
Reads supporting (aligned to +/- strand):  ref base A (6/3);  new base C (106/119);  total (112/123)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 235 total aligned reads displayed.

AATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAA  >  CP009273/705953‑706241
                                                                                                                                                |                                                                                                                                                
aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTc                                                                                                                                               <  1:2452307/148‑1 (MQ=255)
  tttCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCAt                                                                                                                                             >  1:1801367/1‑148 (MQ=255)
     cttcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCg                                                                                                                                          >  1:1105997/1‑148 (MQ=255)
       tcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGa                                                                                                                                        >  1:371610/1‑148 (MQ=255)
          cccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATc                                                                                                                                     <  1:1562659/148‑1 (MQ=255)
             atatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGt                                                                                                                                  >  2:1544797/1‑148 (MQ=255)
             atatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGt                                                                                                                                  <  2:3116062/148‑1 (MQ=255)
               atCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                <  1:3021672/148‑1 (MQ=255)
               atCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                >  2:11708/1‑148 (MQ=255)
               atCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                <  1:3078376/148‑1 (MQ=255)
               atCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                <  2:3105347/148‑1 (MQ=255)
               atCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                <  2:3105927/148‑1 (MQ=255)
               atCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                <  1:1244360/148‑1 (MQ=255)
                  gATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGt                                                                                                                             <  2:258377/148‑1 (MQ=255)
                  gATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGt                                                                                                                             <  2:215467/148‑1 (MQ=255)
                  gATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGt                                                                                                                             <  2:2058012/148‑1 (MQ=255)
                    tCAGACGTTTGTATAAATCGTCCGCACTGACGTCATGGTTGTCCGGCTCCTGAAGAAATACCAGGATGTTTAAACGAGGAAGCGATACTTTCTGGCCAGCATTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTCTTa                                                                                                                           >  2:2305575/1‑148 (MQ=255)
                     cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTAc                                                                                                                          >  1:53095/1‑148 (MQ=255)
                     cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTAc                                                                                                                          <  2:1713486/148‑1 (MQ=255)
                      aGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACt                                                                                                                         >  1:2071363/1‑148 (MQ=255)
                       gACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTa                                                                                                                        <  2:564389/148‑1 (MQ=255)
                         cGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAg                                                                                                                      <  2:884619/148‑1 (MQ=255)
                         cGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTGAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAg                                                                                                                      >  2:2324717/1‑148 (MQ=255)
                         cGTTTGTATAAATCGTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAg                                                                                                                      <  2:136965/148‑1 (MQ=255)
                              gTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGtt                                                                                                                 >  1:1698462/1‑148 (MQ=255)
                              gTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGtt                                                                                                                 >  2:1003795/1‑148 (MQ=255)
                                ataAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCa                                                                                                               <  1:3237316/148‑1 (MQ=255)
                                ataAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCa                                                                                                               <  2:2463984/148‑1 (MQ=255)
                                ataAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCa                                                                                                               <  1:1465945/148‑1 (MQ=255)
                                  aaaTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACt                                                                                                             >  2:2301744/1‑148 (MQ=255)
                                    aTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTc                                                                                                           >  1:3424363/1‑148 (MQ=255)
                                     tCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                          >  2:1834650/1‑148 (MQ=255)
                                     tCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                          <  1:3466572/148‑1 (MQ=255)
                                     tCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                          >  2:890355/1‑148 (MQ=255)
                                      cTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAt                                                                                                         <  1:1849073/148‑1 (MQ=255)
                                        tCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTAAAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTa                                                                                                       >  2:2946045/1‑148 (MQ=255)
                                           gCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTagaa                                                                                                    <  2:1631259/148‑1 (MQ=255)
                                                 aCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                                                              <  1:3209878/148‑1 (MQ=255)
                                                 aCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                                                              >  2:1837025/1‑148 (MQ=255)
                                                 aCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                                                              <  1:2152286/148‑1 (MQ=255)
                                                 aCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                                                              >  1:1625016/1‑148 (MQ=255)
                                                  cGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAACGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGa                                                                                             >  2:2795588/1‑148 (MQ=255)
                                                        ggTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAt                                                                                       <  1:3104126/148‑1 (MQ=255)
                                                            gTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGc                                                                                   <  2:1958208/148‑1 (MQ=255)
                                                             tCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGct                                                                                  <  2:711146/148‑1 (MQ=255)
                                                               cGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctct                                                                                <  1:473218/148‑1 (MQ=255)
                                                                ggCTCCTGAATAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCtt                                                                               <  1:3178906/148‑1 (MQ=255)
                                                                   tCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgag                                                                            <  1:300297/148‑1 (MQ=255)
                                                                    ccTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga                                                                           <  2:324544/148‑1 (MQ=255)
                                                                      tGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATa                                                                         <  2:485141/148‑1 (MQ=255)
                                                                      tGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATa                                                                         <  2:2913963/148‑1 (MQ=255)
                                                                      tGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATa                                                                         <  1:2639684/148‑1 (MQ=255)
                                                                        aagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAt                                                                       >  1:1302968/1‑148 (MQ=255)
                                                                           aaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCg                                                                    <  1:2479293/148‑1 (MQ=255)
                                                                             cTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTa                                                                  >  2:2851469/1‑148 (MQ=255)
                                                                               tCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATc                                                                >  1:217575/1‑148 (MQ=255)
                                                                                 cAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAt                                                              >  1:924192/1‑148 (MQ=255)
                                                                                  aGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAtt                                                             <  1:1003795/148‑1 (MQ=255)
                                                                                  aGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAtt                                                             <  2:2126934/148‑1 (MQ=255)
                                                                                    gATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat                                                           >  2:1686592/1‑148 (MQ=255)
                                                                                    gATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat                                                           <  1:546097/148‑1 (MQ=255)
                                                                                    gATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat                                                           >  2:3406624/1‑148 (MQ=255)
                                                                                     aTTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtata                                                          >  2:3238823/1‑148 (MQ=255)
                                                                                       ttttACACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGa                                                        <  1:2306575/148‑1 (MQ=255)
                                                                                       ttttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGa                                                        <  2:371610/148‑1 (MQ=255)
                                                                                        tttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGaa                                                       <  1:2247937/148‑1 (MQ=255)
                                                                                            aaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGc                                                   >  2:2263040/1‑148 (MQ=255)
                                                                                             aCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGcc                                                  >  2:1649360/1‑148 (MQ=255)
                                                                                                 ggAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGc                                              >  2:1423551/1‑148 (MQ=255)
                                                                                                  gAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGcc                                             >  1:2731583/1‑148 (MQ=255)
                                                                                                   aaGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCt                                            >  2:2187113/1‑148 (MQ=255)
                                                                                                      cGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaaa                                         >  2:129738/1‑148 (MQ=255)
                                                                                                      cGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaaa                                         >  1:3200085/1‑148 (MQ=255)
                                                                                                         tACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa                                      <  1:2327175/148‑1 (MQ=255)
                                                                                                         tACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa                                      <  1:3039604/148‑1 (MQ=255)
                                                                                                         tACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa                                      <  2:2685993/148‑1 (MQ=255)
                                                                                                           cTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaa                                    >  2:3160329/1‑148 (MQ=255)
                                                                                                           cTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGCTAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaa                                    <  1:3385303/148‑1 (MQ=255)
                                                                                                             ttCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAc                                  >  1:743009/1‑148 (MQ=255)
                                                                                                             ttCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAc                                  <  1:1444988/148‑1 (MQ=255)
                                                                                                                    cAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGt                           <  2:2731583/148‑1 (MQ=255)
                                                                                                                    cAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGt                           <  1:1137870/148‑1 (MQ=255)
                                                                                                                     aGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTc                          <  2:346940/148‑1 (MQ=255)
                                                                                                                      gCTTTCTTTAGGGGGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                         <  1:3073842/148‑1 (MQ=255)
                                                                                                                      gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                         >  2:2374589/1‑148 (MQ=255)
                                                                                                                      gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                         >  1:636009/1‑148 (MQ=255)
                                                                                                                      gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                         <  2:125158/148‑1 (MQ=255)
                                                                                                                      gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                         <  2:1926501/148‑1 (MQ=255)
                                                                                                                      gCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                         >  1:802339/1‑148 (MQ=255)
                                                                                                                       ctttctttAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                        >  2:2130207/1‑148 (MQ=255)
                                                                                                                        tttctttAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCt                       >  2:632614/1‑148 (MQ=255)
                                                                                                                         ttctttAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTg                      >  1:2889800/1‑148 (MQ=255)
                                                                                                                           ctttAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGc                    >  1:1466780/1‑148 (MQ=255)
                                                                                                                              tAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCaaa                 >  2:1815896/1‑148 (MQ=255)
                                                                                                                                 ggCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAatat              <  2:1105997/148‑1 (MQ=255)
                                                                                                                                      tATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta         >  1:567694/1‑148 (MQ=255)
                                                                                                                                        ttGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAATAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCAGGCAAATATTGTtaat       <  1:1221838/148‑1 (MQ=255)
                                                                                                                                           ttATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaa    <  2:3424363/148‑1 (MQ=255)
                                                                                                                                            tATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaa   <  2:659340/148‑1 (MQ=255)
                                                                                                                                             aTCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa  <  2:1698779/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAA  >  CP009273/705953‑706241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGG  >  CP009273/705948‑706246
                                                                                                                                                     |                                                                                                                                                     
AGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCC                                                                                                                                                       >  SRR19973066.1365140/1‑150 (MQ=60)
     AATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCAT                                                                                                                                                  <  SRR19973066.2298562/150‑1 (MQ=60)
     AATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCAT                                                                                                                                                  <  SRR19973066.2738880/150‑1 (MQ=60)
     AATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCAT                                                                                                                                                  <  SRR19973066.2469019/150‑1 (MQ=60)
     AATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCAT                                                                                                                                                  >  SRR19973066.1814167/1‑150 (MQ=60)
      ATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATG                                                                                                                                                 <  SRR19973066.2617260/150‑1 (MQ=60)
      ATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATG                                                                                                                                                 >  SRR19973066.1884040/1‑150 (MQ=60)
        TTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCG                                                                                                                                               >  SRR19973066.1114011/1‑150 (MQ=60)
          CTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGA                                                                                                                                             >  SRR19973066.374521/1‑150 (MQ=60)
               CCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTG                                                                                                                                        <  SRR19973066.1573911/150‑1 (MQ=60)
               CCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTG                                                                                                                                        >  SRR19973066.3274610/1‑150 (MQ=60)
                CCATCTCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGT                                                                                                                                       <  SRR19973066.2400911/150‑1 (MQ=60)
                  ATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCC                                                                                                                                     >  SRR19973066.3561461/1‑150 (MQ=60)
                    ATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTG                                                                                                                                   <  SRR19973066.3052457/150‑1 (MQ=60)
                    ATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTG                                                                                                                                   <  SRR19973066.1253278/150‑1 (MQ=60)
                    ATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTG                                                                                                                                   <  SRR19973066.3041830/150‑1 (MQ=60)
                    ATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTG                                                                                                                                   >  SRR19973066.349645/1‑150 (MQ=60)
                    ATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTG                                                                                                                                   <  SRR19973066.3098940/150‑1 (MQ=60)
                        ATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTAC                                                                                                                               >  SRR19973066.53766/1‑150 (MQ=60)
                         TCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACT                                                                                                                              >  SRR19973066.2085697/1‑150 (MQ=60)
                         TCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACT                                                                                                                              <  SRR19973066.1113298/150‑1 (MQ=60)
                           AGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAA                                                                                                                            >  SRR19973066.541155/1‑150 (MQ=60)
                            GACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAG                                                                                                                           <  SRR19973066.1653326/150‑1 (MQ=60)
                               GTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGG                                                                                                                        >  SRR19973066.633941/1‑150 (MQ=60)
                                 TTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTT                                                                                                                      >  SRR19973066.1710805/1‑150 (MQ=60)
                                     ATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACT                                                                                                                  <  SRR19973066.1477403/150‑1 (MQ=60)
                                     ATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACT                                                                                                                  <  SRR19973066.3032140/150‑1 (MQ=60)
                                     ATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACT                                                                                                                  <  SRR19973066.3258688/150‑1 (MQ=60)
                                     ATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACT                                                                                                                  <  SRR19973066.3034344/150‑1 (MQ=60)
                                     ATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACT                                                                                                                  >  SRR19973066.3074938/1‑150 (MQ=60)
                                     ATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACT                                                                                                                  <  SRR19973066.1476250/150‑1 (MQ=60)
                                     ATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACT                                                                                                                  <  SRR19973066.1476290/150‑1 (MQ=60)
                                       AAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTC                                                                                                                >  SRR19973066.3447265/1‑150 (MQ=60)
                                       AAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGGATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTC                                                                                                                >  SRR19973066.3447182/1‑150 (MQ=60)
                                        AATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCA                                                                                                               <  SRR19973066.2137240/150‑1 (MQ=60)
                                          TCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATT                                                                                                             <  SRR19973066.3489740/150‑1 (MQ=60)
                                          TCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATT                                                                                                             <  SRR19973066.1715429/150‑1 (MQ=60)
                                          TCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATT                                                                                                             <  SRR19973066.3317698/150‑1 (MQ=60)
                                           CTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTA                                                                                                            <  SRR19973066.1862309/150‑1 (MQ=60)
                                            TTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAG                                                                                                           >  SRR19973066.218555/1‑150 (MQ=60)
                                                 CACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAA                                                                                                      <  SRR19973066.3236160/150‑1 (MQ=60)
                                                    TGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTG                                                                                                   <  SRR19973066.3097109/150‑1 (MQ=60)
                                                    TGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTG                                                                                                   >  SRR19973066.1636798/1‑150 (MQ=60)
                                                     GACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGA                                                                                                  <  SRR19973066.269144/150‑1 (MQ=60)
                                                      ACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAC                                                                                                 <  SRR19973066.3231157/150‑1 (MQ=60)
                                                      ACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAC                                                                                                 <  SRR19973066.2167229/150‑1 (MQ=60)
                                                      ACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAC                                                                                                 <  SRR19973066.3230979/150‑1 (MQ=60)
                                                           ATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAT                                                                                            >  SRR19973066.1247332/1‑150 (MQ=60)
                                                             GGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTT                                                                                          <  SRR19973066.3124858/150‑1 (MQ=60)
                                                             GGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTT                                                                                          >  SRR19973066.77409/1‑150 (MQ=60)
                                                                    CGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTG                                                                                   <  SRR19973066.3113225/150‑1 (MQ=60)
                                                                    CGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTG                                                                                   >  SRR19973066.2434784/1‑150 (MQ=60)
                                                                    CGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTG                                                                                   <  SRR19973066.476801/150‑1 (MQ=60)
                                                                     GGCTCCTGAATAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGA                                                                                  <  SRR19973066.3200057/150‑1 (MQ=60)
                                                                        TCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAT                                                                               <  SRR19973066.302413/150‑1 (MQ=60)
                                                                           TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGTGCGGTATTTATATCCGTCATGAGTAATCTGTCCTGTTACTAAGAGGTTCAATTCATTATAAGAAGTGACAGAATTTGATCTTGAGATAAT                                                                            >  SRR19973066.3096884/1‑150 (MQ=60)
                                                                           TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAT                                                                            >  SRR19973066.1312357/1‑150 (MQ=60)
                                                                           TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAT                                                                            >  SRR19973066.1312409/1‑150 (MQ=60)
                                                                           TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAT                                                                            <  SRR19973066.2657444/150‑1 (MQ=60)
                                                                                AACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTA                                                                       <  SRR19973066.944479/150‑1 (MQ=60)
                                                                                AACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTA                                                                       <  SRR19973066.2496209/150‑1 (MQ=60)
                                                                                  CTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATC                                                                     >  SRR19973066.3018283/1‑150 (MQ=60)
                                                                                  CTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATC                                                                     >  SRR19973066.219360/1‑150 (MQ=60)
                                                                                    TCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAT                                                                   >  SRR19973066.930865/1‑150 (MQ=60)
                                                                                       AGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                                                                <  SRR19973066.1011188/150‑1 (MQ=60)
                                                                                        GGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATA                                                               <  SRR19973066.397430/150‑1 (MQ=60)
                                                                                         GATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAG                                                              <  SRR19973066.550034/150‑1 (MQ=60)
                                                                                         GATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAG                                                              >  SRR19973066.505868/1‑150 (MQ=60)
                                                                                         GATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAG                                                              >  SRR19973066.505899/1‑150 (MQ=60)
                                                                                          ATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGA                                                             >  SRR19973066.2135083/1‑150 (MQ=60)
                                                                                            TTTTACACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAT                                                           <  SRR19973066.2322373/150‑1 (MQ=60)
                                                                                            TTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAT                                                           <  SRR19973066.2322458/150‑1 (MQ=60)
                                                                                             TTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATT                                                          <  SRR19973066.2263435/150‑1 (MQ=60)
                                                                                                 AACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCA                                                      >  SRR19973066.1612157/1‑150 (MQ=60)
                                                                                                  ACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCAC                                                     <  SRR19973066.557421/150‑1 (MQ=60)
                                                                                                     AGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCC                                                  >  SRR19973066.2749960/1‑150 (MQ=60)
                                                                                                       GAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCTTATCATTATAGAATTGCCACGCCTA                                                >  SRR19973066.1380092/1‑150 (MQ=60)
                                                                                                         AGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAA                                              >  SRR19973066.3221332/1‑150 (MQ=60)
                                                                                                             TTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAA                                          <  SRR19973066.321613/150‑1 (MQ=60)
                                                                                                              TACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAA                                         <  SRR19973066.2343060/150‑1 (MQ=60)
                                                                                                              TACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAA                                         <  SRR19973066.3059919/150‑1 (MQ=60)
                                                                                                                CTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAC                                       >  SRR19973066.2207542/1‑150 (MQ=60)
                                                                                                                CTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAC                                       >  SRR19973066.748491/1‑150 (MQ=60)
                                                                                                                CTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAC                                       <  SRR19973066.1223890/150‑1 (MQ=60)
                                                                                                                CTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAC                                       >  SRR19973066.748524/1‑150 (MQ=60)
                                                                                                                CTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGCTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAC                                       <  SRR19973066.3407893/150‑1 (MQ=60)
                                                                                                                  TTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCA                                     <  SRR19973066.1455264/150‑1 (MQ=60)
                                                                                                                        CCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTC                               >  SRR19973066.2760285/1‑150 (MQ=60)
                                                                                                                        CCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTC                               >  SRR19973066.3206733/1‑150 (MQ=60)
                                                                                                                         CAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCC                              <  SRR19973066.1146013/150‑1 (MQ=60)
                                                                                                                         CAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCC                              >  SRR19973066.640589/1‑150 (MQ=60)
                                                                                                                         CAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCC                              >  SRR19973066.808236/1‑150 (MQ=60)
                                                                                                                         CAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCC                              >  SRR19973066.808355/1‑150 (MQ=60)
                                                                                                                           GCTTTCTTTAGGGGGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCT                            <  SRR19973066.3094384/150‑1 (MQ=60)
                                                                                                                           GCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGATTTGCCACGCCTAAATGAAAACCACAAGTCCCT                            <  SRR19973066.1493196/150‑1 (MQ=60)
                                                                                                                            CTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTG                           >  SRR19973066.2909125/1‑150 (MQ=60)
                                                                                                                            CTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCAATTAATTAGAATATGTGAAAGAATTTGCTCTTGAGTTAATGCGTATCATTATAGATTTGCCACGCCTAAATGAAAACCACAAGTCCCTG                           <  SRR19973066.890807/150‑1 (MQ=60)
                                                                                                                             TTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGG                          <  SRR19973066.3449552/150‑1 (MQ=60)
                                                                                                                              TTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGC                         >  SRR19973066.1476617/1‑150 (MQ=60)
                                                                                                                              TTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGC                         >  SRR19973066.1477132/1‑150 (MQ=60)
                                                                                                                                  TTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAAT                     >  SRR19973066.1743332/1‑150 (MQ=60)
                                                                                                                                   TAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATA                    >  SRR19973066.3144280/1‑150 (MQ=60)
                                                                                                                                     GGGCGTTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTATAATAAGTGACAGAATTTGCTCTTGAGATAATGCTTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATT                  <  SRR19973066.621598/150‑1 (MQ=60)
                                                                                                                                     GGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATT                  <  SRR19973066.1130849/150‑1 (MQ=60)
                                                                                                                                      GGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTG                 <  SRR19973066.2309401/150‑1 (MQ=60)
                                                                                                                                         GGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTA              >  SRR19973066.571714/1‑150 (MQ=60)
                                                                                                                                          GTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAA             >  SRR19973066.1443919/1‑150 (MQ=60)
                                                                                                                                            ATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATA           <  SRR19973066.249214/150‑1 (MQ=60)
                                                                                                                                             TTGTTATCCGTCTTGCGGAATCTGTCCTGTTACTACGCGTTTCACTTCATTAGAAGAAGTGACATAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAA          <  SRR19973066.1230433/150‑1 (MQ=60)
                                                                                                                                             TTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAATAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCAGGCAAATATTGTTAATAA          <  SRR19973066.1230465/150‑1 (MQ=60)
                                                                                                                                                 TATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAC      >  SRR19973066.720785/1‑150 (MQ=60)
                                                                                                                                                     CGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGG  >  SRR19973066.659712/1‑150 (MQ=60)
                                                                                                                                                     CGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGG  <  SRR19973066.2935590/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGG  >  CP009273/705948‑706246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑