Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 706,097 A→C 100% T2T (ACT→ACG) 
*29G (TGA→GGA) 		 fur ←
uof ←		 ferric iron uptake regulon transcriptional repressor; autorepressor
ryhB‑regulated fur leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273706,0970AC98.0% 836.9 / ‑0.4 215T2T (ACT→ACG) 
*29G (TGA→GGA) 		fur
uof		ferric iron uptake regulon transcriptional repressor; autorepressor
ryhB‑regulated fur leader peptide
Reads supporting (aligned to +/- strand):  ref base A (0/5);  new base C (90/120);  total (90/125)
Fisher's exact test for biased strand distribution p-value = 7.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 215 total aligned reads displayed.

CAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAC  >  CP009273/705952‑706242
                                                                                                                                                 |                                                                                                                                                 
cAATTTCTTCACCCAGATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAAAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTc                                                                                                                                                <  2:2494419/149‑1 (MQ=255)
 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCa                                                                                                                                               >  1:1066025/1‑149 (MQ=255)
 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCa                                                                                                                                               <  2:2383464/149‑1 (MQ=255)
 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCa                                                                                                                                               <  2:1291332/149‑1 (MQ=255)
 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCa                                                                                                                                               >  1:3171083/1‑149 (MQ=255)
 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCa                                                                                                                                               <  1:1534704/149‑1 (MQ=255)
  aTTTCTTCACCCAGATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCAt                                                                                                                                              <  1:3935212/149‑1 (MQ=255)
   tttCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATg                                                                                                                                             >  1:3391737/1‑149 (MQ=255)
   tttCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATg                                                                                                                                             <  1:3172723/149‑1 (MQ=255)
     tcttcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGGTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCg                                                                                                                                           >  1:815056/1‑149 (MQ=255)
       ttcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGa                                                                                                                                         >  1:125987/1‑149 (MQ=255)
        tcccccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGaa                                                                                                                                        <  2:1175061/146‑1 (MQ=255)
          aCCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATc                                                                                                                                      <  2:2070687/149‑1 (MQ=255)
          aCCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATc                                                                                                                                      <  1:336834/149‑1 (MQ=255)
           cccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCt                                                                                                                                     >  1:1118292/1‑149 (MQ=255)
           cccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCt                                                                                                                                     <  2:724508/149‑1 (MQ=255)
              atatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTc                                                                                                                                  >  1:3683729/1‑149 (MQ=255)
               tatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                 <  1:3687664/149‑1 (MQ=255)
               tatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                 <  2:1315292/149‑1 (MQ=255)
                atCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCt                                                                                                                                >  2:3556099/1‑149 (MQ=255)
                   gATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGtt                                                                                                                             <  1:1481389/149‑1 (MQ=255)
                      cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACt                                                                                                                          >  2:3787564/1‑149 (MQ=255)
                      cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACt                                                                                                                          >  1:1875561/1‑149 (MQ=255)
                      cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACt                                                                                                                          <  2:2265986/149‑1 (MQ=255)
                       aGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTa                                                                                                                         >  1:1664513/1‑149 (MQ=255)
                       aGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTa                                                                                                                         >  2:1351886/1‑149 (MQ=255)
                        gACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTaa                                                                                                                        <  2:3989626/149‑1 (MQ=255)
                         aCGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAg                                                                                                                       <  2:3322225/149‑1 (MQ=255)
                            tttGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCgg                                                                                                                    >  1:1326972/1‑149 (MQ=255)
                              tGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGtt                                                                                                                  <  1:2623288/149‑1 (MQ=255)
                                tataAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCa                                                                                                                <  1:2902381/149‑1 (MQ=255)
                                  taAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACt                                                                                                              >  2:400069/1‑149 (MQ=255)
                                     aTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                           >  2:1488735/1‑149 (MQ=255)
                                     aTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                           <  1:340095/149‑1 (MQ=255)
                                     aTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                           <  2:829946/149‑1 (MQ=255)
                                           cGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTagaa                                                                                                     >  1:27560/1‑149 (MQ=255)
                                                 gACGGGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                                                               <  2:3150668/149‑1 (MQ=255)
                                                  aCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGa                                                                                              >  2:2445760/1‑149 (MQ=255)
                                                      gATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCTGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGa                                                                                          <  2:1045918/149‑1 (MQ=255)
                                                      gATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGa                                                                                          >  2:3873833/1‑149 (MQ=255)
                                                       aTGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGaa                                                                                         >  1:3907431/1‑149 (MQ=255)
                                                       aTGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGaa                                                                                         <  1:1994471/149‑1 (MQ=255)
                                                         ggTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAtt                                                                                       >  1:2296371/1‑149 (MQ=255)
                                                          gTTGTCCGGCTCCTGAAGAACTACCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGAGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAATTTAAAGAAttt                                                                                      >  1:2101517/1‑149 (MQ=255)
                                                            tGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGc                                                                                    <  1:400244/149‑1 (MQ=255)
                                                            tGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGc                                                                                    <  2:417038/149‑1 (MQ=255)
                                                              tCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctc                                                                                  <  2:3627041/149‑1 (MQ=255)
                                                                        gaagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAt                                                                        <  2:1426925/149‑1 (MQ=255)
                                                                         aagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATg                                                                       >  2:2845829/1‑149 (MQ=255)
                                                                         aagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATg                                                                       >  1:1717630/1‑149 (MQ=255)
                                                                              cTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                                                                  >  2:2591661/1‑149 (MQ=255)
                                                                                tCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCa                                                                >  2:1976973/1‑149 (MQ=255)
                                                                                tCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCa                                                                <  1:3157291/149‑1 (MQ=255)
                                                                                 ccAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAt                                                               <  2:233490/149‑1 (MQ=255)
                                                                                  cAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAtt                                                              <  2:2964288/149‑1 (MQ=255)
                                                                                   aGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATta                                                             <  1:2699430/149‑1 (MQ=255)
                                                                                    ggATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat                                                            <  2:3728731/149‑1 (MQ=255)
                                                                                    ggATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat                                                            >  2:652494/1‑149 (MQ=255)
                                                                                     gATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtata                                                           >  2:3091243/1‑149 (MQ=255)
                                                                                      aTTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAATAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAg                                                          <  1:594370/149‑1 (MQ=255)
                                                                                        ttttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGaa                                                        <  2:3419467/149‑1 (MQ=255)
                                                                                        ttttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGaa                                                        >  2:1837677/1‑149 (MQ=255)
                                                                                           tAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTg                                                     <  2:101541/149‑1 (MQ=255)
                                                                                            aagcGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGc                                                    <  2:2732919/146‑1 (MQ=255)
                                                                                            aaaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGc                                                    <  2:671405/149‑1 (MQ=255)
                                                                                            aaaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGc                                                    <  2:1618136/149‑1 (MQ=255)
                                                                                                  ggAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGcc                                              <  1:2461734/149‑1 (MQ=255)
                                                                                                     aGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaa                                           >  2:3059909/1‑149 (MQ=255)
                                                                                                      gCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaaa                                          <  2:3892372/149‑1 (MQ=255)
                                                                                                        gTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATg                                        >  2:1795496/1‑149 (MQ=255)
                                                                                                         ttACGTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGGTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGCATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa                                       <  2:1970000/149‑1 (MQ=255)
                                                                                                                cAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACcac                                <  1:2981077/149‑1 (MQ=255)
                                                                                                                 aGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACcaca                               >  2:2743076/1‑149 (MQ=255)
                                                                                                                  ggCCATCTTTCTTTAGGGGGGTATTGTTATCCTTAATGCGGTATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCTTATCATTATAGAATTGCCACGCCTAAATGAAAACCACaa                              <  1:3502019/149‑1 (MQ=255)
                                                                                                                  ggCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACaa                              <  1:686133/149‑1 (MQ=255)
                                                                                                                    ccAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGt                            <  2:208864/149‑1 (MQ=255)
                                                                                                                     cAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTc                           <  2:2619661/149‑1 (MQ=255)
                                                                                                                      aggtttctttAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                          <  1:2624977/146‑1 (MQ=255)
                                                                                                                      aGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                          >  2:708406/1‑149 (MQ=255)
                                                                                                                       gCTTTCTTTAGGGCGTTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         <  1:3037308/149‑1 (MQ=255)
                                                                                                                       gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCTTTAGAAGAAGTGACATAATTTGCTCTTGAGATATTGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         <  1:185841/149‑1 (MQ=255)
                                                                                                                       gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         <  2:1166902/149‑1 (MQ=255)
                                                                                                                       gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         <  2:1163302/149‑1 (MQ=255)
                                                                                                                       gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCACTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         >  2:4027040/1‑149 (MQ=255)
                                                                                                                        ctttctttAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCt                        <  1:2391298/149‑1 (MQ=255)
                                                                                                                                aGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAata                <  2:3391737/149‑1 (MQ=255)
                                                                                                                                 gggCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTAGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAatat               >  2:2846119/1‑149 (MQ=255)
                                                                                                                                  ggCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt              <  1:193905/149‑1 (MQ=255)
                                                                                                                                  ggCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt              >  1:1391979/1‑149 (MQ=255)
                                                                                                                                   gCGGTTTTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTTAGATAATGCGTATCATTATATAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTg             <  2:2421594/149‑1 (MQ=255)
                                                                                                                                    cGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTAATTAGAAGAAGTTACAGAATTTGCTCTTGAGATAATGCTTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGt            <  1:918001/149‑1 (MQ=255)
                                                                                                                                     ggTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt           >  2:503685/1‑149 (MQ=255)
                                                                                                                                     ggTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt           >  2:1565709/1‑149 (MQ=255)
                                                                                                                                      gTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta          >  1:3964365/1‑149 (MQ=255)
                                                                                                                                         ttGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaata       >  1:2954172/1‑149 (MQ=255)
                                                                                                                                             tATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa   <  1:3727286/149‑1 (MQ=255)
                                                                                                                                             tATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa   >  2:956116/1‑149 (MQ=255)
                                                                                                                                             tATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa   <  2:1664513/149‑1 (MQ=255)
                                                                                                                                              aTCCGTCATGCGTATTCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAc  <  1:2773885/149‑1 (MQ=255)
                                                                                                                                              aTCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAc  <  1:2220467/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAC  >  CP009273/705952‑706242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGG  >  CP009273/705948‑706246
                                                                                                                                                     |                                                                                                                                                     
AGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCC                                                                                                                                                       >  SRR19973068.2084111/1‑150 (MQ=60)
    CAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAATCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCA                                                                                                                                                   >  SRR19973068.3190427/1‑150 (MQ=60)
    CAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCA                                                                                                                                                   >  SRR19973068.1073012/1‑150 (MQ=60)
    CAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCA                                                                                                                                                   >  SRR19973068.3190586/1‑150 (MQ=60)
     AATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCAT                                                                                                                                                  <  SRR19973068.1544830/150‑1 (MQ=60)
     AATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCAT                                                                                                                                                  <  SRR19973068.1544803/150‑1 (MQ=60)
      ATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATG                                                                                                                                                 >  SRR19973068.3412284/1‑150 (MQ=60)
      ATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATG                                                                                                                                                 >  SRR19973068.1485358/1‑150 (MQ=60)
      ATTTCTTCACCCAGATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATG                                                                                                                                                 <  SRR19973068.3959040/150‑1 (MQ=60)
       TTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGC                                                                                                                                                <  SRR19973068.3192239/150‑1 (MQ=60)
        TTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGGTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCG                                                                                                                                               >  SRR19973068.820605/1‑150 (MQ=60)
          CTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGA                                                                                                                                             >  SRR19973068.127154/1‑150 (MQ=60)
              ACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCT                                                                                                                                         <  SRR19973068.338975/150‑1 (MQ=60)
              ACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCT                                                                                                                                         <  SRR19973068.338964/150‑1 (MQ=60)
              ACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCT                                                                                                                                         >  SRR19973068.1125761/1‑150 (MQ=60)
                 CATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTC                                                                                                                                      >  SRR19973068.3111018/1‑150 (MQ=60)
                 CATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTC                                                                                                                                      >  SRR19973068.3705962/1‑150 (MQ=60)
                 CATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTC                                                                                                                                      >  SRR19973068.3705955/1‑150 (MQ=60)
                   TATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCT                                                                                                                                    <  SRR19973068.3709924/150‑1 (MQ=60)
                   TATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCT                                                                                                                                    <  SRR19973068.3709900/150‑1 (MQ=60)
                       GATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTA                                                                                                                                <  SRR19973068.1491128/150‑1 (MQ=60)
                         TCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACT                                                                                                                              >  SRR19973068.1887981/1‑150 (MQ=60)
                         TCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACT                                                                                                                              >  SRR19973068.2422989/1‑150 (MQ=60)
                          CAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTA                                                                                                                             >  SRR19973068.1675272/1‑150 (MQ=60)
                               GTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGG                                                                                                                        >  SRR19973068.1335580/1‑150 (MQ=60)
                                  TGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTC                                                                                                                     <  SRR19973068.2639801/150‑1 (MQ=60)
                                  TGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTC                                                                                                                     <  SRR19973068.2639794/150‑1 (MQ=60)
                                    TATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCAC                                                                                                                   <  SRR19973068.2920440/150‑1 (MQ=60)
                                     ATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACT                                                                                                                  >  SRR19973068.3190873/1‑150 (MQ=60)
                                         ATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAT                                                                                                              <  SRR19973068.342251/150‑1 (MQ=60)
                                         ATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAT                                                                                                              <  SRR19973068.342216/150‑1 (MQ=60)
                                         ATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAT                                                                                                              <  SRR19973068.342236/150‑1 (MQ=60)
                                           CTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAGTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTA                                                                                                            >  SRR19973068.2636152/1‑150 (MQ=60)
                                            TTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAG                                                                                                           <  SRR19973068.2265835/150‑1 (MQ=60)
                                              CCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTCAGCGGTTCACTTCATTAGAA                                                                                                         >  SRR19973068.28023/1‑150 (MQ=60)
                                              CCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAA                                                                                                         >  SRR19973068.27940/1‑150 (MQ=60)
                                                   CTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGT                                                                                                    <  SRR19973068.1594466/150‑1 (MQ=60)
                                                    TGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTG                                                                                                   <  SRR19973068.2227720/150‑1 (MQ=60)
                                                    TGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTG                                                                                                   <  SRR19973068.138427/150‑1 (MQ=60)
                                                        GTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAG                                                                                               >  SRR19973068.1912282/1‑150 (MQ=60)
                                                         TGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGA                                                                                              <  SRR19973068.1200264/150‑1 (MQ=60)
                                                         TGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGA                                                                                              >  SRR19973068.1539810/1‑150 (MQ=60)
                                                          GATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAA                                                                                             >  SRR19973068.3931072/1‑150 (MQ=60)
                                                          GATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAA                                                                                             >  SRR19973068.1881195/1‑150 (MQ=60)
                                                           ATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAT                                                                                            <  SRR19973068.2007620/150‑1 (MQ=60)
                                                            TGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATT                                                                                           >  SRR19973068.2311229/1‑150 (MQ=60)
                                                             GGTTGTCCGGCTCCTGAAGAACTACCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGAGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAATTTAAAGAATTT                                                                                          >  SRR19973068.2115217/1‑150 (MQ=60)
                                                                TGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCT                                                                                       <  SRR19973068.403050/150‑1 (MQ=60)
                                                                   CCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTT                                                                                    <  SRR19973068.1105479/150‑1 (MQ=60)
                                                                    CGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTG                                                                                   <  SRR19973068.3980468/150‑1 (MQ=60)
                                                                       CTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGA                                                                                >  SRR19973068.2110443/1‑150 (MQ=60)
                                                                        TCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAT                                                                               <  SRR19973068.2959729/150‑1 (MQ=60)
                                                                        TCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAT                                                                               <  SRR19973068.3595632/150‑1 (MQ=60)
                                                                         CCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATA                                                                              >  SRR19973068.3846927/1‑150 (MQ=60)
                                                                            GAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATG                                                                           >  SRR19973068.1728565/1‑150 (MQ=60)
                                                                             AAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGC                                                                          <  SRR19973068.633671/150‑1 (MQ=60)
                                                                                AACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTA                                                                       >  SRR19973068.2727643/1‑150 (MQ=60)
                                                                                   TTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCA                                                                    <  SRR19973068.2689202/150‑1 (MQ=60)
                                                                                    TCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAT                                                                   <  SRR19973068.394642/150‑1 (MQ=60)
                                                                                    TCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAT                                                                   <  SRR19973068.3176692/150‑1 (MQ=60)
                                                                                       ATGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCATCTTTCTTTAGGGCGGTATTGTTATCCGTCATTCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                                                                <  SRR19973068.2716177/150‑1 (MQ=60)
                                                                                       AGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                                                                <  SRR19973068.2716192/150‑1 (MQ=60)
                                                                                       AGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                                                                <  SRR19973068.3340267/150‑1 (MQ=60)
                                                                                          ATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAATAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGA                                                             <  SRR19973068.598386/150‑1 (MQ=60)
                                                                                               TAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGC                                                        >  SRR19973068.1961382/1‑150 (MQ=60)
                                                                                                    GAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGC                                                   >  SRR19973068.575401/1‑150 (MQ=60)
                                                                                                      GGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCT                                                 <  SRR19973068.2477442/150‑1 (MQ=60)
                                                                                                              TACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAA                                         >  SRR19973068.132899/1‑150 (MQ=60)
                                                                                                              TACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAGTATAGAATTGCCACGCCTAAATGAAA                                         >  SRR19973068.2220085/1‑150 (MQ=60)
                                                                                                                   TCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCAC                                    <  SRR19973068.1750866/150‑1 (MQ=60)
                                                                                                                    CAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACA                                   <  SRR19973068.2999522/150‑1 (MQ=60)
                                                                                                                      GGCCATCTTTCTTTAGGGGGGTATTGTTATCCTTAATGCGGTATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCTTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAG                                 <  SRR19973068.3523302/150‑1 (MQ=60)
                                                                                                                      GGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAG                                 <  SRR19973068.691251/150‑1 (MQ=60)
                                                                                                                      GGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAG                                 <  SRR19973068.3530571/150‑1 (MQ=60)
                                                                                                                      GGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAG                                 <  SRR19973068.690472/150‑1 (MQ=60)
                                                                                                                         CAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCC                              >  SRR19973068.4022255/1‑150 (MQ=60)
                                                                                                                          AGGTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCC                             <  SRR19973068.2641488/150‑1 (MQ=60)
                                                                                                                           GCTTTCTTTTGGGCGGTTTTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCTTTAGAAGAAGTGACATAATTTGCTCTTGAGATATTGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCT                            <  SRR19973068.187309/150‑1 (MQ=60)
                                                                                                                           GCTTTCTTTAGGGCGTTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCT                            <  SRR19973068.3055956/150‑1 (MQ=60)
                                                                                                                           GCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCT                            <  SRR19973068.3055872/150‑1 (MQ=60)
                                                                                                                           GCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCT                            <  SRR19973068.3055960/150‑1 (MQ=60)
                                                                                                                            CTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTG                           <  SRR19973068.2406537/150‑1 (MQ=60)
                                                                                                                               TCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCA                        <  SRR19973068.2361650/150‑1 (MQ=60)
                                                                                                                                     GGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATT                  >  SRR19973068.1400846/1‑150 (MQ=60)
                                                                                                                                      GGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCAGTTAATTAGAATAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATTAAAACCACAAGTCCCTGGCAAATATTG                 <  SRR19973068.2792126/150‑1 (MQ=60)
                                                                                                                                      GGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTG                 <  SRR19973068.195419/150‑1 (MQ=60)
                                                                                                                                        CGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTAATTAGAAGAAGTTACAGAATTTGCTCTTGAGATAATGCTTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTT               <  SRR19973068.924179/150‑1 (MQ=60)
                                                                                                                                         GGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTA              >  SRR19973068.3988345/1‑150 (MQ=60)
                                                                                                                                         GGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTA              <  SRR19973068.3995882/150‑1 (MQ=60)
                                                                                                                                            ATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATA           >  SRR19973068.2972530/1‑150 (MQ=60)
                                                                                                                                            ATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATA           >  SRR19973068.2972496/1‑150 (MQ=60)
                                                                                                                                                 TATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAATAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCTTTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAC      <  SRR19973068.1692748/150‑1 (MQ=60)
                                                                                                                                                 TATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAC      <  SRR19973068.3749659/150‑1 (MQ=60)
                                                                                                                                                 TATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAC      <  SRR19973068.83530/150‑1 (MQ=60)
                                                                                                                                                  ATCCGTCATGCGTATTCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACG     <  SRR19973068.2791010/150‑1 (MQ=60)
                                                                                                                                                  ATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACG     >  SRR19973068.2658261/1‑150 (MQ=60)
                                                                                                                                                  ATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACG     >  SRR19973068.2658186/1‑150 (MQ=60)
                                                                                                                                                  ATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACG     <  SRR19973068.2234984/150‑1 (MQ=60)
                                                                                                                                                     CGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGG  >  SRR19973068.3871717/1‑150 (MQ=60)
                                                                                                                                                     CGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGG  >  SRR19973068.2574249/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGG  >  CP009273/705948‑706246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑