Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F394 I0 R1	1342	215.0	7134094	96.2%	6862998	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	726,087	G→T	100%	R350L (CGT→CTT)	rhsC →	Rhs family putative polymorphic toxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	726,087	0	G	T	96.2%	550.2 / 12.1	186	R350L (CGT→CTT)	rhsC	Rhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand): ref base G (2/5); new base T (101/78); total (103/83)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

TACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAG  >  CP009273/725942‑726230

tACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTc                                                                                                                                               <  2:1068042/148‑1 (MQ=255)

 aCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTca                                                                                                                                              >  1:332024/1‑148 (MQ=255)

      gagaATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACgg                                                                                                                                         >  2:2056097/1‑148 (MQ=255)

           tttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGa                                                                                                                                    >  1:2575600/1‑148 (MQ=255)

           tttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGa                                                                                                                                    >  1:1085679/1‑148 (MQ=255)

           tttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGa                                                                                                                                    >  1:487678/1‑148 (MQ=255)

           tttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGa                                                                                                                                    <  1:2897714/148‑1 (MQ=255)

            ttACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGAc                                                                                                                                   >  1:2977522/1‑148 (MQ=255)

               ccTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCgg                                                                                                                                >  1:813117/1‑148 (MQ=255)

               ccTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCgg                                                                                                                                >  1:813105/1‑148 (MQ=255)

               ccTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCgg                                                                                                                                >  1:3366164/1‑148 (MQ=255)

                   ccgcgccgcTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGAGCCGACCGGAAAt                                                                                                                            <  1:2478800/148‑1 (MQ=255)

                    cgcgccgcTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATc                                                                                                                           <  2:1179219/148‑1 (MQ=255)

                       gccgcTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGt                                                                                                                        <  1:3181966/148‑1 (MQ=255)

                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   >  1:143139/1‑148 (MQ=255)

                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   >  1:142889/1‑148 (MQ=255)

                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   <  1:622970/148‑1 (MQ=255)

                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   <  2:2379866/148‑1 (MQ=255)

                            tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                   <  2:471315/148‑1 (MQ=255)

                             ggTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGt                                                                                                                  >  1:104502/1‑148 (MQ=255)

                             ggTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGt                                                                                                                  >  1:103844/1‑148 (MQ=255)

                              gTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGtt                                                                                                                 <  2:23743/148‑1 (MQ=255)

                              gTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGAGAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGtt                                                                                                                 <  2:264360/148‑1 (MQ=255)

                                gcgcTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTAc                                                                                                               >  1:1839683/1‑148 (MQ=255)

                                       ggCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCg                                                                                                        >  1:1510339/1‑148 (MQ=255)

                                       ggCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCg                                                                                                        <  2:2878166/148‑1 (MQ=255)

                                        gCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGa                                                                                                       <  2:3237939/148‑1 (MQ=255)

                                        gCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGa                                                                                                       <  2:3237995/148‑1 (MQ=255)

                                        gCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGa                                                                                                       <  1:1148186/148‑1 (MQ=255)

                                          tGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcg                                                                                                     <  2:673714/148‑1 (MQ=255)

                                          tGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcg                                                                                                     <  2:127014/148‑1 (MQ=255)

                                           ggACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgg                                                                                                    <  2:1173621/148‑1 (MQ=255)

                                            gACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcggg                                                                                                   >  2:2558489/1‑148 (MQ=255)

                                            gACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcggg                                                                                                   >  2:1675848/1‑148 (MQ=255)

                                             aCGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgggc                                                                                                  >  1:221104/1‑148 (MQ=255)

                                               gCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgggcgg                                                                                                <  2:1522880/148‑1 (MQ=255)

                                                 cccGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGt                                                                                              >  2:957104/1‑148 (MQ=255)

                                                  ccGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTg                                                                                             <  1:2056018/148‑1 (MQ=255)

                                                  ccGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTg                                                                                             <  1:2056097/148‑1 (MQ=255)

                                                   cgcgGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGa                                                                                            <  1:2331122/148‑1 (MQ=255)

                                                        cgAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGaa                                                                                       <  1:2959928/148‑1 (MQ=255)

                                                          aaCTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACa                                                                                     >  1:998120/1‑148 (MQ=255)

                                                            cTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGc                                                                                   >  1:3100935/1‑148 (MQ=255)

                                                               gcggcgGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTaa                                                                                >  2:2741817/1‑148 (MQ=255)

                                                               gcggcgGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTaa                                                                                >  1:1361872/1‑148 (MQ=255)

                                                                  gcggTGTATGACCGCAGCAATACACAGGTGCTCAGATTTACTTACGATGATAAATACGGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAAcc                                                                             <  1:1637901/148‑1 (MQ=255)

                                                                      tgtATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGc                                                                         >  2:3518540/1‑148 (MQ=255)

                                                                       gtATGACCGCAGCAACACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCa                                                                        <  1:844859/148‑1 (MQ=255)

                                                                            aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGTAGGCtt                                                                   <  2:3108479/148‑1 (MQ=255)

                                                                            aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGTAGGCtt                                                                   <  2:2824279/148‑1 (MQ=255)

                                                                            aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCtt                                                                   <  2:451397/148‑1 (MQ=255)

                                                                            aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCtt                                                                   <  1:1233505/148‑1 (MQ=255)

                                                                                 agcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGtt                                                              <  1:235307/148‑1 (MQ=255)

                                                                                  gcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGTCGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTa                                                             <  2:142889/148‑1 (MQ=255)

                                                                                  gcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTa                                                             <  1:1509087/148‑1 (MQ=255)

                                                                                  gcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTa                                                             <  1:2438026/148‑1 (MQ=255)

                                                                                  gcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTa                                                             <  2:143139/148‑1 (MQ=255)

                                                                                   caATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTac                                                            <  1:1192332/148‑1 (MQ=255)

                                                                                   caATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTac                                                            >  2:2893629/1‑148 (MQ=255)

                                                                                    aaTACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTaca                                                           <  2:813105/148‑1 (MQ=255)

                                                                                     aTACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTacac                                                          >  1:568270/1‑148 (MQ=255)

                                                                                      tACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACg                                                         <  1:381687/148‑1 (MQ=255)

                                                                                       acacAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGt                                                        >  1:878695/1‑148 (MQ=38)

                                                                                       acacAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGt                                                        >  1:2665940/1‑148 (MQ=38)

                                                                                        cacaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTa                                                       >  2:378205/1‑148 (MQ=38)

                                                                                         acaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTAt                                                      >  2:3408698/1‑148 (MQ=37)

                                                                                         acaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTAt                                                      >  2:1217294/1‑148 (MQ=37)

                                                                                         acaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTAt                                                      >  2:1463305/1‑148 (MQ=37)

                                                                                             gTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGt                                                  >  1:3517022/1‑148 (MQ=37)

                                                                                              tGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTa                                                 <  1:2969773/148‑1 (MQ=37)

                                                                                                  cAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATgag                                             >  1:173402/1‑148 (MQ=37)

                                                                                                   aGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATgaga                                            <  2:1649855/148‑1 (MQ=37)

                                                                                                   aGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATgaga                                            <  1:2255266/148‑1 (MQ=37)

                                                                                                       ttacttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGa                                        <  1:2891283/148‑1 (MQ=255)

                                                                                                         acttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGAcc                                      >  1:127498/1‑148 (MQ=255)

                                                                                                           ttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGc                                    >  1:77320/1‑148 (MQ=255)

                                                                                                             acGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcat                                  <  1:284755/148‑1 (MQ=255)

                                                                                                               gatgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatca                                <  2:121192/148‑1 (MQ=255)

                                                                                                               gatgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatca                                <  2:2054921/148‑1 (MQ=255)

                                                                                                               gatgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatca                                <  2:3193194/148‑1 (MQ=255)

                                                                                                                atgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcac                               >  1:190174/1‑148 (MQ=255)

                                                                                                                atgatAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcac                               >  2:2205457/1‑148 (MQ=39)

                                                                                                                 tgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcacc                              >  1:2295384/1‑148 (MQ=255)

                                                                                                                        tACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCg                       <  2:2694439/148‑1 (MQ=39)

                                                                                                                             gggCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGc                  >  1:1466301/1‑148 (MQ=255)

                                                                                                                              ggCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGcc                 >  2:133605/1‑148 (MQ=255)

                                                                                                                               gCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCt                >  2:17051/1‑148 (MQ=255)

                                                                                                                                  ggATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGaa             >  1:2789860/1‑148 (MQ=255)

                                                                                                                                  ggATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGaa             >  1:2791753/1‑148 (MQ=255)

                                                                                                                                  ggATGGTCGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGATACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGaa             >  1:157050/1‑148 (MQ=255)

                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGACGGACCGCATCACCATCACCGACAGCCTGAAc            >  2:2990125/1‑148 (MQ=255)

                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAc            >  2:2450389/1‑148 (MQ=255)

                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAc            >  1:1055540/1‑148 (MQ=255)

                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAc            >  1:1066893/1‑148 (MQ=255)

                                                                                                                                   gATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAc            >  1:1069736/1‑148 (MQ=255)

                                                                                                                                         ggCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGt      >  2:482420/1‑148 (MQ=255)

                                                                                                                                             cACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAg  >  2:1092733/1‑148 (MQ=255)

                                                                                                                                             cACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAg  >  2:943004/1‑148 (MQ=255)

                                                                                                                                             cACCGTCACACGGGCCGAGCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAg  <  2:2392412/148‑1 (MQ=255)

                                                                                                                                             cACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAg  <  2:3116251/148‑1 (MQ=255)

TACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAG  >  CP009273/725942‑726230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCT  >  CP009273/725939‑726234

GAATACCCGGAGAATTGACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTT                                                                                                                                                    <  SRR19973066.302487/150‑1 (MQ=60)

GAATACCCGGAGAATTGACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTT                                                                                                                                                    <  SRR19973066.302498/150‑1 (MQ=60)

  ATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATCAATACCGGGGCCGGATGGGGGCGCACCTTCA                                                                                                                                                  >  SRR19973066.2143524/1‑150 (MQ=60)

  ATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCA                                                                                                                                                  >  SRR19973066.334560/1‑150 (MQ=60)

        GGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGG                                                                                                                                            <  SRR19973066.2619046/150‑1 (MQ=60)

           GAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCG                                                                                                                                         >  SRR19973066.612576/1‑150 (MQ=60)

            AATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGA                                                                                                                                        >  SRR19973066.2593072/1‑150 (MQ=60)

            AATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGA                                                                                                                                        >  SRR19973066.1093560/1‑150 (MQ=60)

            AATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGA                                                                                                                                        >  SRR19973066.1093603/1‑150 (MQ=60)

            AATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGA                                                                                                                                        >  SRR19973066.491335/1‑150 (MQ=60)

             ATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGAC                                                                                                                                       >  SRR19973066.2997199/1‑150 (MQ=60)

              TTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACC                                                                                                                                      <  SRR19973066.2917079/150‑1 (MQ=60)

                TACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGG                                                                                                                                    >  SRR19973066.3388607/1‑150 (MQ=60)

                TACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGG                                                                                                                                    >  SRR19973066.819076/1‑150 (MQ=60)

                TACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGG                                                                                                                                    >  SRR19973066.819064/1‑150 (MQ=60)

                    TGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAAT                                                                                                                                >  SRR19973066.2966818/1‑150 (MQ=60)

                      CCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGAGCCGACCGGAAATCT                                                                                                                              <  SRR19973066.2495713/150‑1 (MQ=60)

                       CGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTG                                                                                                                             <  SRR19973066.150593/150‑1 (MQ=60)

                          GCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTA                                                                                                                          <  SRR19973066.3203133/150‑1 (MQ=60)

                             GCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCG                                                                                                                       <  SRR19973066.2569671/150‑1 (MQ=60)

                             GCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCG                                                                                                                       >  SRR19973066.144262/1‑150 (MQ=60)

                             GCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCG                                                                                                                       >  SRR19973066.144491/1‑150 (MQ=60)

                             GCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCG                                                                                                                       >  SRR19973066.144512/1‑150 (MQ=60)

                              CTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGT                                                                                                                      >  SRR19973066.104936/1‑150 (MQ=60)

                              CTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGT                                                                                                                      >  SRR19973066.105597/1‑150 (MQ=60)

                               TGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTT                                                                                                                     <  SRR19973066.627406/150‑1 (MQ=60)

                                 GTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTAC                                                                                                                   >  SRR19973066.1852830/1‑150 (MQ=60)

                                    CGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGAC                                                                                                                <  SRR19973066.768939/150‑1 (MQ=60)

                                        ATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCG                                                                                                            >  SRR19973066.1520908/1‑150 (MQ=60)

                                         TGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGA                                                                                                           <  SRR19973066.860792/150‑1 (MQ=60)

                                           GCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACG                                                                                                         <  SRR19973066.1156378/150‑1 (MQ=60)

                                             TGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGG                                                                                                       <  SRR19973066.3398350/150‑1 (MQ=60)

                                              GGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGC                                                                                                      >  SRR19973066.222909/1‑150 (MQ=60)

                                              GGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGC                                                                                                      >  SRR19973066.222901/1‑150 (MQ=60)

                                              GGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGC                                                                                                      >  SRR19973066.908226/1‑150 (MQ=60)

                                              GGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGC                                                                                                      >  SRR19973066.2034406/1‑150 (MQ=60)

                                                  GCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGT                                                                                                  <  SRR19973066.3319233/150‑1 (MQ=60)

                                                    CCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGA                                                                                                >  SRR19973066.1530782/1‑150 (MQ=60)

                                                     CCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGAC                                                                                               <  SRR19973066.2070285/150‑1 (MQ=60)

                                                     CCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGAC                                                                                               <  SRR19973066.2070364/150‑1 (MQ=60)

                                                      CGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACA                                                                                              <  SRR19973066.2347030/150‑1 (MQ=60)

                                                          GCGAACTGGCGGCGGTGTATGACCGCAGCAATACACTGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAAC                                                                                          <  SRR19973066.2762715/150‑1 (MQ=60)

                                                           CGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACA                                                                                         >  SRR19973066.1005472/1‑150 (MQ=60)

                                                           CGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACA                                                                                         <  SRR19973066.2979535/150‑1 (MQ=60)

                                                             AACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGC                                                                                       >  SRR19973066.3121648/1‑150 (MQ=60)

                                                                TGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAA                                                                                    >  SRR19973066.1371722/1‑150 (MQ=60)

                                                                     GCGGTGTATGACCGCAGCAATACACAGGTGCTCAGATTTACTTACGATGATAAATACGGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCG                                                                               <  SRR19973066.1649812/150‑1 (MQ=60)

                                                                         TGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAG                                                                           >  SRR19973066.69673/1‑150 (MQ=60)

                                                                          GTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGG                                                                          <  SRR19973066.850780/150‑1 (MQ=59)

                                                                          GTATGACCGCAGCAACACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGG                                                                          <  SRR19973066.850991/150‑1 (MQ=47)

                                                                               ACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAA                                                                     >  SRR19973066.2366517/1‑150 (MQ=48)

                                                                               ACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAA                                                                     <  SRR19973066.1242270/150‑1 (MQ=48)

                                                                                CCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAG                                                                    >  SRR19973066.787697/1‑150 (MQ=46)

                                                                                 CGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGT                                                                   >  SRR19973066.3158526/1‑150 (MQ=46)

                                                                                    AGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTAC                                                                <  SRR19973066.237162/150‑1 (MQ=46)

                                                                                     GCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACA                                                               <  SRR19973066.1519632/150‑1 (MQ=46)

                                                                                     GCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACA                                                               <  SRR19973066.2454633/150‑1 (MQ=46)

                                                                                     GCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACA                                                               <  SRR19973066.2454629/150‑1 (MQ=46)

                                                                                      CAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACAC                                                              >  SRR19973066.1161317/1‑150 (MQ=44)

                                                                                      CAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACAC                                                              <  SRR19973066.1200675/150‑1 (MQ=44)

                                                                                      CAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACAC                                                              >  SRR19973066.572275/1‑150 (MQ=44)

                                                                                      CAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACAC                                                              >  SRR19973066.572292/1‑150 (MQ=44)

                                                                                        ATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGT                                                            >  SRR19973066.2683783/1‑150 (MQ=40)

                                                                                        ATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGT                                                            >  SRR19973066.1621498/1‑150 (MQ=40)

                                                                                        ATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGT                                                            >  SRR19973066.884956/1‑150 (MQ=40)

                                                                                         TACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTA                                                           <  SRR19973066.384701/150‑1 (MQ=38)

                                                                                           CACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATC                                                         <  SRR19973066.1665989/150‑1 (MQ=34)

                                                                                             CAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAG                                                       >  SRR19973066.1145041/1‑150 (MQ=32)

                                                                                              AGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGT                                                      >  SRR19973066.3540448/1‑150 (MQ=32)

                                                                                                 TGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATG                                                   <  SRR19973066.2989401/150‑1 (MQ=32)

                                                                                                 TGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATG                                                   <  SRR19973066.2991036/150‑1 (MQ=32)

                                                                                                 TGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATG                                                   <  SRR19973066.2989408/150‑1 (MQ=32)

                                                                                                   CGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAG                                                 >  SRR19973066.174944/1‑150 (MQ=32)

                                                                                                      AGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAG                                              <  SRR19973066.1460095/150‑1 (MQ=34)

                                                                                                      AGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAG                                              <  SRR19973066.1330809/150‑1 (MQ=34)

                                                                                                      AGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAG                                              <  SRR19973066.2270811/150‑1 (MQ=34)

                                                                                                          TTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACC                                          >  SRR19973066.1421760/1‑150 (MQ=42)

                                                                                                          TTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACC                                          >  SRR19973066.128757/1‑150 (MQ=42)

                                                                                                          TTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACC                                          <  SRR19973066.2910615/150‑1 (MQ=42)

                                                                                                            ACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGC                                        >  SRR19973066.126802/1‑150 (MQ=42)

                                                                                                            ACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGC                                        >  SRR19973066.78225/1‑150 (MQ=42)

                                                                                                                ACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCA                                    <  SRR19973066.286811/150‑1 (MQ=42)

                                                                                                                 CGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCAC                                   >  SRR19973066.191813/1‑150 (MQ=42)

                                                                                                                  GATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACC                                  >  SRR19973066.2311121/1‑150 (MQ=42)

                                                                                                                  GATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACC                                  >  SRR19973066.2311123/1‑150 (MQ=42)

                                                                                                                    TGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCAT                                <  SRR19973066.3035343/150‑1 (MQ=42)

                                                                                                                      ATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCA                              >  SRR19973066.1780677/1‑150 (MQ=42)

                                                                                                                              CGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGC                      >  SRR19973066.1476651/1‑150 (MQ=42)

                                                                                                                                 GGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTG                   <  SRR19973066.3566217/150‑1 (MQ=42)

                                                                                                                                   CCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAA                 >  SRR19973066.2808636/1‑150 (MQ=46)

                                                                                                                                   CCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAA                 >  SRR19973066.2279016/1‑150 (MQ=46)

                                                                                                                                   CCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAA                 >  SRR19973066.2808686/1‑150 (MQ=46)

                                                                                                                                   CCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAA                 >  SRR19973066.2810537/1‑150 (MQ=46)

                                                                                                                                   CCGGATGGTCGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGATACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAA                 >  SRR19973066.158496/1‑150 (MQ=44)

                                                                                                                                    CGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGACC                >  SRR19973066.2868393/1‑150 (MQ=44)

                                                                                                                                    CGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAC                >  SRR19973066.1887995/1‑150 (MQ=48)

                                                                                                                                    CGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAC                >  SRR19973066.1077580/1‑150 (MQ=48)

                                                                                                                                    CGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAC                >  SRR19973066.1077508/1‑150 (MQ=48)

                                                                                                                                    CGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAC                >  SRR19973066.1074716/1‑150 (MQ=48)

                                                                                                                                    CGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAC                >  SRR19973066.1062062/1‑150 (MQ=48)

                                                                                                                                    CGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAC                >  SRR19973066.1063299/1‑150 (MQ=48)

                                                                                                                                     GGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACC               >  SRR19973066.390133/1‑150 (MQ=50)

                                                                                                                                         GGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCG           >  SRR19973066.1072997/1‑150 (MQ=53)

                                                                                                                                          GTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGT          <  SRR19973066.2841949/150‑1 (MQ=53)

                                                                                                                                                  CCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCT  >  SRR19973066.557021/1‑150 (MQ=59)

                                                                                                                                                  CCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCT  <  SRR19973066.1802042/150‑1 (MQ=59)

GAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCT  >  CP009273/725939‑726234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`