Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 892,358 G→T 100% V257L (GTG→TTG)  potH → putrescine transporter subunit: membrane component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273892,3580GT99.0% 641.3 / ‑3.2 196V257L (GTG→TTG) potHputrescine transporter subunit: membrane component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (100/94);  total (102/94)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 198 total aligned reads displayed.

TCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGG  >  CP009273/892213‑892501
                                                                                                                                                 |                                                                                                                                               
tCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTtttg                                                                                                                                               >  1:2350970/1‑148 (MQ=255)
 cGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGa                                                                                                                                              <  2:637003/148‑1 (MQ=255)
 cGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGa                                                                                                                                              <  2:1086730/148‑1 (MQ=255)
   tATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTACCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATc                                                                                                                                            <  2:3032945/148‑1 (MQ=255)
     ttGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATccc                                                                                                                                          >  2:1086965/1‑148 (MQ=255)
      tGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCg                                                                                                                                         >  2:765453/1‑148 (MQ=255)
      tGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCg                                                                                                                                         >  1:436220/1‑148 (MQ=255)
      tGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCg                                                                                                                                         >  2:3417242/1‑148 (MQ=255)
         ttattCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGaa                                                                                                                                      >  2:2257965/1‑148 (MQ=255)
         ttattCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACCACCGCTGAAAAAGTTCTTTACTGAGATCGTGCCGCTGACTAAAGGTGGGATTATTGACGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGATTTTGATCCCGGaa                                                                                                                                      >  2:2307985/1‑148 (MQ=255)
              cGCTGGTAGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCt                                                                                                                                 <  1:1061936/148‑1 (MQ=255)
                 tggtggAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTACAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCgg                                                                                                                              <  2:444520/148‑1 (MQ=255)
                  ggtggAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGt                                                                                                                             >  1:2816384/1‑148 (MQ=255)
                          cagcGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGa                                                                                                                     <  1:1564552/148‑1 (MQ=255)
                            gcgcTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACa                                                                                                                   >  2:1924092/1‑148 (MQ=255)
                                 ggATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGcatc                                                                                                              <  2:2511804/148‑1 (MQ=255)
                                  gATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGcatca                                                                                                             >  1:1887149/1‑148 (MQ=255)
                                  gATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGcatca                                                                                                             >  2:1490264/1‑148 (MQ=255)
                                   aTCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGcatcat                                                                                                            <  2:3080888/148‑1 (MQ=255)
                                   aTCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGcatcat                                                                                                            >  2:3082607/1‑148 (MQ=255)
                                    tctcGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATg                                                                                                           <  2:371506/148‑1 (MQ=255)
                                         gTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCgg                                                                                                      >  1:2775319/1‑148 (MQ=255)
                                         gTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCgg                                                                                                      <  1:348977/148‑1 (MQ=255)
                                          ggCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTACAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCggg                                                                                                     <  2:207277/147‑1 (MQ=255)
                                                aCCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTTCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTg                                                                                               <  2:3564954/148‑1 (MQ=255)
                                                aCCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTg                                                                                               <  2:144983/148‑1 (MQ=255)
                                                 ccGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGc                                                                                              <  1:2496711/148‑1 (MQ=255)
                                                 ccGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGc                                                                                              <  2:1221365/148‑1 (MQ=255)
                                                  cGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCt                                                                                             <  2:1589887/148‑1 (MQ=255)
                                                    cTGAAAACGTTCTTTAGTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTAt                                                                                           <  2:1949099/148‑1 (MQ=255)
                                                    cTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTAt                                                                                           <  1:2395993/148‑1 (MQ=255)
                                                      gAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATgg                                                                                         >  2:2327272/1‑148 (MQ=255)
                                                      gAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATgg                                                                                         <  1:1366202/148‑1 (MQ=255)
                                                       aaaaCGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGc                                                                                        >  2:1571467/1‑148 (MQ=255)
                                                       aaaaCGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGc                                                                                        <  1:3363350/148‑1 (MQ=255)
                                                          aCGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAag                                                                                     >  1:2123933/1‑148 (MQ=255)
                                                           cGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAaga                                                                                    >  2:1720651/1‑148 (MQ=255)
                                                           cGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAaga                                                                                    <  1:3531953/148‑1 (MQ=255)
                                                           cGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAaga                                                                                    >  2:943606/1‑148 (MQ=255)
                                                             ttCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGt                                                                                  <  2:2792760/148‑1 (MQ=255)
                                                               cTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGttt                                                                                <  1:894756/148‑1 (MQ=255)
                                                               cTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGttt                                                                                <  1:656623/148‑1 (MQ=255)
                                                                   aCTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCa                                                                            <  1:1490379/148‑1 (MQ=255)
                                                                   aCTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCa                                                                            <  1:1928312/148‑1 (MQ=255)
                                                                    cTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCaa                                                                           <  1:516159/148‑1 (MQ=255)
                                                                     tgtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAt                                                                          <  1:3230504/148‑1 (MQ=255)
                                                                     tgtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAt                                                                          <  2:436220/148‑1 (MQ=255)
                                                                         aTCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAAcc                                                                      >  2:1218071/1‑148 (MQ=255)
                                                                            gTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACcgcg                                                                   <  2:2430771/148‑1 (MQ=255)
                                                                            gTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACcgcg                                                                   <  2:2266276/148‑1 (MQ=255)
                                                                            gTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACcgcg                                                                   <  1:2704701/148‑1 (MQ=255)
                                                                             tGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGa                                                                  <  2:2821983/148‑1 (MQ=255)
                                                                                  cTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGt                                                                                  <  1:538526/127‑1 (MQ=255)
                                                                                  cTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGt                                                                                  >  2:538526/1‑127 (MQ=255)
                                                                                    gACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCg                                                           <  1:1306827/148‑1 (MQ=255)
                                                                                     aCTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCgg                                                          >  2:3088910/1‑148 (MQ=255)
                                                                                     aCTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCgg                                                          >  1:1851915/1‑148 (MQ=255)
                                                                                      cTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGt                                                         >  2:1257174/1‑148 (MQ=255)
                                                                                      cTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGt                                                         <  1:3008571/148‑1 (MQ=255)
                                                                                       tAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGTCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTg                                                        >  1:2665516/1‑148 (MQ=255)
                                                                                        aaaGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTgg                                                       >  1:3150731/1‑148 (MQ=255)
                                                                                         aaGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGc                                                      >  1:386561/1‑148 (MQ=255)
                                                                                           ggTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCt                                                    <  2:2743173/148‑1 (MQ=255)
                                                                                            gTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTc                                                   <  1:833393/148‑1 (MQ=255)
                                                                                             tGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTcg                                                  <  1:3125655/148‑1 (MQ=255)
                                                                                              gggATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTcgg                                                 >  1:2908243/1‑148 (MQ=255)
                                                                                                    attGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAg                                           >  1:1739542/1‑148 (MQ=255)
                                                                                                      tGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCg                                         >  2:2588865/1‑148 (MQ=255)
                                                                                                       gCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGa                                        <  1:160471/148‑1 (MQ=255)
                                                                                                         cGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatc                                      <  2:1255820/148‑1 (MQ=255)
                                                                                                         cGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatc                                      <  1:1397978/148‑1 (MQ=255)
                                                                                                         cGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatc                                      >  1:3448629/1‑148 (MQ=255)
                                                                                                           gATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGGCAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatcat                                    <  2:2663304/148‑1 (MQ=255)
                                                                                                           gATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatcat                                    <  2:2609847/148‑1 (MQ=255)
                                                                                                            aTCGATTCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatcatc                                   <  1:2348201/148‑1 (MQ=255)
                                                                                                              cGATGCTGGTGTTTATCCCGTCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATcatcat                                 >  1:1210431/1‑148 (MQ=255)
                                                                                                              cGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATcatcat                                 >  2:2164101/1‑148 (MQ=255)
                                                                                                              cGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATcatcat                                 >  1:2866008/1‑148 (MQ=255)
                                                                                                                aTGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGt                               >  1:1503154/1‑148 (MQ=255)
                                                                                                                aTGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGt                               >  1:2917264/1‑148 (MQ=255)
                                                                                                                  gCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtg                             <  2:1177846/148‑1 (MQ=255)
                                                                                                                  gCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtg                             <  1:2634506/148‑1 (MQ=255)
                                                                                                                     ggTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACCGCATCATGATCGGGCGCGTGCTCTGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgctg                          >  2:494876/1‑148 (MQ=255)
                                                                                                                      gtgtTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgctgc                         <  1:1086965/148‑1 (MQ=255)
                                                                                                                               ccGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATtg                >  2:2933072/1‑148 (MQ=255)
                                                                                                                               ccGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGACAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATtg                >  1:2102032/1‑148 (MQ=255)
                                                                                                                                cGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATtgt               <  1:2031397/148‑1 (MQ=255)
                                                                                                                                cGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATtgt               >  1:2966858/1‑148 (MQ=255)
                                                                                                                                 ggCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATtgtg              <  1:2257965/148‑1 (MQ=255)
                                                                                                                                  gCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGc             <  2:2964288/148‑1 (MQ=255)
                                                                                                                                   cTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGcc            >  1:99784/1‑148 (MQ=255)
                                                                                                                                     gtgGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGa          >  2:2508183/1‑148 (MQ=255)
                                                                                                                                     gtgGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGa          <  1:2378466/148‑1 (MQ=255)
                                                                                                                                      tgGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGAt         >  2:2345371/1‑148 (MQ=255)
                                                                                                                                       gggCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATa        >  2:3174589/1‑148 (MQ=255)
                                                                                                                                        ggCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATaa       <  2:861399/148‑1 (MQ=255)
                                                                                                                                        ggCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATaa       >  1:836474/1‑148 (MQ=255)
                                                                                                                                         gCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAAt      >  2:3514055/1‑148 (MQ=255)
                                                                                                                                           gAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAAtgt    <  2:2123933/148‑1 (MQ=255)
                                                                                                                                           gAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAAtgt    <  1:1123903/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGG  >  CP009273/892213‑892501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGG  >  CP009273/892210‑892501
                                                                                                                                                    |                                                                                                                                               
GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTT                                                                                                                                                <  SRR19973066.1009245/150‑1 (MQ=60)
GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTT                                                                                                                                                <  SRR19973066.1009405/150‑1 (MQ=60)
 ATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTG                                                                                                                                               >  SRR19973066.2366941/1‑150 (MQ=60)
     GTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCC                                                                                                                                           >  SRR19973066.342600/1‑150 (MQ=60)
      TATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCC                                                                                                                                          >  SRR19973066.1194230/1‑150 (MQ=60)
      TATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCC                                                                                                                                          >  SRR19973066.1584349/1‑150 (MQ=60)
       ATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCG                                                                                                                                         >  SRR19973066.3246011/1‑150 (MQ=60)
       ATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCG                                                                                                                                         >  SRR19973066.439598/1‑150 (MQ=60)
                 CGCTGGTAGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCG                                                                                                                               <  SRR19973066.1069723/150‑1 (MQ=60)
                   CTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGT                                                                                                                             >  SRR19973066.2835319/1‑150 (MQ=60)
                     GGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTACAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGG                                                                                                                           <  SRR19973066.1120253/150‑1 (MQ=60)
                         GAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCG                                                                                                                       <  SRR19973066.3048258/150‑1 (MQ=60)
                          AAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGG                                                                                                                      >  SRR19973066.3589435/1‑150 (MQ=60)
                            GCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGAC                                                                                                                    >  SRR19973066.2269512/1‑150 (MQ=60)
                             CAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACA                                                                                                                   <  SRR19973066.1575822/150‑1 (MQ=60)
                                   TGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCA                                                                                                             >  SRR19973066.1900459/1‑150 (MQ=60)
                                   TGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCA                                                                                                             >  SRR19973066.1900602/1‑150 (MQ=60)
                                        CTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATC                                                                                                        >  SRR19973066.2476216/1‑150 (MQ=60)
                                          CGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGG                                                                                                      >  SRR19973066.2794249/1‑150 (MQ=60)
                                          CGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGG                                                                                                      >  SRR19973066.2794002/1‑150 (MQ=60)
                                            GTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGC                                                                                                    <  SRR19973066.351701/150‑1 (MQ=60)
                                              GCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGATGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGC                                                                                                  >  SRR19973066.2348226/1‑150 (MQ=60)
                                                ACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGT                                                                                                <  SRR19973066.1836335/150‑1 (MQ=60)
                                                 CGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTG                                                                                               >  SRR19973066.3019464/1‑150 (MQ=60)
                                                 CGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTG                                                                                               <  SRR19973066.417965/150‑1 (MQ=60)
                                                    CCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCNA                                                                                            <  SRR19973066.2513727/150‑1 (MQ=60)
                                                      GCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATG                                                                                          <  SRR19973066.865208/150‑1 (MQ=60)
                                                       CTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGG                                                                                         <  SRR19973066.2412139/150‑1 (MQ=60)
                                                        TGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGC                                                                                        >  SRR19973066.315700/1‑150 (MQ=60)
                                                         GAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCA                                                                                       <  SRR19973066.1376081/150‑1 (MQ=60)
                                                          AAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAA                                                                                      <  SRR19973066.3385772/150‑1 (MQ=60)
                                                          AAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAA                                                                                      >  SRR19973066.2715119/1‑150 (MQ=60)
                                                           AAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAG                                                                                     >  SRR19973066.1707023/1‑150 (MQ=60)
                                                           AAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAG                                                                                     >  SRR19973066.2138636/1‑150 (MQ=60)
                                                              CGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGT                                                                                  >  SRR19973066.2086141/1‑150 (MQ=60)
                                                              CGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGT                                                                                  <  SRR19973066.3555439/150‑1 (MQ=60)
                                                              CGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGT                                                                                  <  SRR19973066.488255/150‑1 (MQ=60)
                                                                acgtgtgctcttctgatctCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTT                                                                                <  SRR19973066.542438/131‑1 (MQ=60)
                                                                  CTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTT                                                                              <  SRR19973066.661413/150‑1 (MQ=60)
                                                                  CTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTT                                                                              <  SRR19973066.901085/150‑1 (MQ=60)
                                                                      ACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAT                                                                          <  SRR19973066.1942018/150‑1 (MQ=60)
                                                                      ACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAT                                                                          <  SRR19973066.1937773/150‑1 (MQ=60)
                                                                      ACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAT                                                                          <  SRR19973066.1500811/150‑1 (MQ=60)
                                                                      ACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAT                                                                          <  SRR19973066.1501176/150‑1 (MQ=60)
                                                                       CTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATA                                                                         <  SRR19973066.519965/150‑1 (MQ=60)
                                                                        TGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAA                                                                        <  SRR19973066.2944091/150‑1 (MQ=60)
                                                                        TGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAA                                                                        <  SRR19973066.3251845/150‑1 (MQ=60)
                                                                         GTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAAC                                                                       >  SRR19973066.937729/1‑150 (MQ=60)
                                                                               GTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGAC                                                                 <  SRR19973066.2722829/150‑1 (MQ=60)
                                                                               GTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGAC                                                                 <  SRR19973066.2723429/150‑1 (MQ=60)
                                                                                    GCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCC                                                            >  SRR19973066.839678/1‑150 (MQ=60)
                                                                                      TGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGG                                                          >  SRR19973066.1865168/1‑150 (MQ=60)
                                                                                       GACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGT                                                         <  SRR19973066.1316305/150‑1 (MQ=60)
                                                                                        ACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGTCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTG                                                        >  SRR19973066.2683359/1‑150 (MQ=60)
                                                                                         CTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGG                                                       >  SRR19973066.1386320/1‑150 (MQ=60)
                                                                                         CTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGG                                                       >  SRR19973066.3171736/1‑150 (MQ=60)
                                                                                         CTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGG                                                       <  SRR19973066.3028593/150‑1 (MQ=60)
                                                                                          TAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGC                                                      >  SRR19973066.389625/1‑150 (MQ=60)
                                                                                             AGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTC                                                   >  SRR19973066.1226862/1‑150 (MQ=60)
                                                                                              GGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCG                                                  <  SRR19973066.2543799/150‑1 (MQ=60)
                                                                                              GGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCG                                                  <  SRR19973066.521813/150‑1 (MQ=60)
                                                                                               GTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGG                                                 >  SRR19973066.2927659/1‑150 (MQ=60)
                                                                                               GTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGG                                                 >  SRR19973066.2927675/1‑150 (MQ=60)
                                                                                               GTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGG                                                 <  SRR19973066.839466/150‑1 (MQ=60)
                                                                                               GTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGG                                                 <  SRR19973066.839470/150‑1 (MQ=60)
                                                                                                TGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGC                                                <  SRR19973066.3146526/150‑1 (MQ=60)
                                                                                                     TTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAG                                           >  SRR19973066.1752091/1‑150 (MQ=60)
                                                                                                       ATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCG                                         >  SRR19973066.133434/1‑150 (MQ=60)
                                                                                                          GCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATC                                      >  SRR19973066.574348/1‑150 (MQ=60)
                                                                                                          GCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATC                                      >  SRR19973066.2376266/1‑150 (MQ=60)
                                                                                                          GCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATC                                      <  SRR19973066.1500696/150‑1 (MQ=60)
                                                                                                          GCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATC                                      <  SRR19973066.161938/150‑1 (MQ=60)
                                                                                                          GCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATC                                      >  SRR19973066.3471687/1‑150 (MQ=60)
                                                                                                            CGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCAT                                    <  SRR19973066.1408054/150‑1 (MQ=60)
                                                                                                               ATCGATTCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCAT                                 <  SRR19973066.2364166/150‑1 (MQ=60)
                                                                                                               ATCGATGCTGGTGTTTATCCCGTCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCAT                                 >  SRR19973066.1218904/1‑150 (MQ=60)
                                                                                                               ATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCAT                                 >  SRR19973066.2885239/1‑150 (MQ=60)
                                                                                                               ATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCAT                                 >  SRR19973066.2670768/1‑150 (MQ=60)
                                                                                                               ATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCAT                                 <  SRR19973066.2364241/150‑1 (MQ=60)
                                                                                                               ATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCAT                                 >  SRR19973066.2886696/1‑150 (MQ=60)
                                                                                                               ATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCAT                                 >  SRR19973066.2884865/1‑150 (MQ=60)
                                                                                                                 CGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGT                               >  SRR19973066.2936723/1‑150 (MQ=60)
                                                                                                                 CGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGT                               >  SRR19973066.1513623/1‑150 (MQ=60)
                                                                                                                     GCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCT                           <  SRR19973066.2652245/150‑1 (MQ=60)
                                                                                                                       TGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGC                         >  SRR19973066.3503617/1‑150 (MQ=60)
                                                                                                                         GTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTG                       <  SRR19973066.1094895/150‑1 (MQ=60)
                                                                                                                                TCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGACAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTG                >  SRR19973066.2116507/1‑150 (MQ=60)
                                                                                                                                TCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGACAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTG                >  SRR19973066.2116515/1‑150 (MQ=60)
                                                                                                                                 CCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGT               >  SRR19973066.2986483/1‑150 (MQ=60)
                                                                                                                                   CGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGC             >  SRR19973066.2035807/1‑150 (MQ=60)
                                                                                                                                   CGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGC             <  SRR19973066.2045580/150‑1 (MQ=60)
                                                                                                                                    GGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCC            >  SRR19973066.100847/1‑150 (MQ=60)
                                                                                                                                    GGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCC            <  SRR19973066.2273520/150‑1 (MQ=60)
                                                                                                                                      CTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGA          >  SRR19973066.3541086/1‑150 (MQ=60)
                                                                                                                                       TGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGAT         <  SRR19973066.3263827/150‑1 (MQ=60)
                                                                                                                                        GTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATA        <  SRR19973066.2394521/150‑1 (MQ=60)
                                                                                                                                        GTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATA        <  SRR19973066.2396917/150‑1 (MQ=60)
                                                                                                                                         TNGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAA       >  SRR19973066.1545873/1‑150 (MQ=60)
                                                                                                                                         TGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAA       >  SRR19973066.842570/1‑150 (MQ=60)
                                                                                                                                              GAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGG  <  SRR19973066.1131994/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                               
GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGG  >  CP009273/892210‑892501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑