Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	892,358	G→T	100%	V257L (GTG→TTG)	potH →	putrescine transporter subunit: membrane component of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	892,358	0	G	T	97.9%	610.3 / 3.4	193	V257L (GTG→TTG)	potH	putrescine transporter subunit: membrane component of ABC superfamily
Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (104/85); total (106/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 196 total aligned reads displayed.

TCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTG  >  CP009273/892213‑892500

tCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTtttg                                                                                                                                              <  1:3453142/148‑1 (MQ=255)

   tATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATc                                                                                                                                           <  1:2519913/148‑1 (MQ=255)

    aTTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATcc                                                                                                                                          >  1:2425647/1‑148 (MQ=255)

    aTTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATcc                                                                                                                                          >  1:2823255/1‑148 (MQ=255)

      tGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCg                                                                                                                                        >  2:1019765/1‑148 (MQ=255)

      tGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCg                                                                                                                                        >  2:333682/1‑148 (MQ=255)

      tGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCg                                                                                                                                        >  2:1899753/1‑148 (MQ=255)

         ttattCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGaa                                                                                                                                     >  1:3248860/1‑148 (MQ=255)

         ttattCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGaa                                                                                                                                     <  1:3158739/148‑1 (MQ=255)

         ttattCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGaa                                                                                                                                     <  1:1891011/148‑1 (MQ=255)

         ttattCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACAGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGaa                                                                                                                                     >  1:2743748/1‑148 (MQ=255)

          tattCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAAc                                                                                                                                    >  2:2198501/1‑148 (MQ=255)

          tattCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACGAAAGGTGGGCTTATTGCCTGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAAc                                                                                                                                    >  1:3059371/1‑148 (MQ=255)

            ttCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTg                                                                                                                                  >  1:2947756/1‑148 (MQ=255)

            ttCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTg                                                                                                                                  >  2:2456197/1‑148 (MQ=255)

             tCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGc                                                                                                                                 >  1:825157/1‑148 (MQ=255)

              cGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCt                                                                                                                                <  2:1563218/148‑1 (MQ=255)

               gCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTc                                                                                                                               <  1:3355266/148‑1 (MQ=255)

                  ggtggAAGGAGCGCTGGATCTCTGTGCACGACCGCTGAAAACGTTCTTTCCTGGGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGCTTCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCggg                                                                                                                            <  1:962305/148‑2 (MQ=255)

                         gcagcGCTGGATCTCGGTGCAAGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCgg                                                                                                                     <  2:3359461/148‑1 (MQ=255)

                          cagcGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGa                                                                                                                    >  2:440017/1‑148 (MQ=255)

                            gcgcTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACa                                                                                                                  >  2:3123744/1‑148 (MQ=255)

                             cgcTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAg                                                                                                                 <  1:757929/148‑1 (MQ=255)

                              gcTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGc                                                                                                                <  1:2310408/148‑1 (MQ=255)

                               cTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGca                                                                                                               <  1:1197408/148‑1 (MQ=255)

                                 ggATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGcatc                                                                                                             >  1:429969/1‑148 (MQ=255)

                                    tctcGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATg                                                                                                          <  2:2129653/148‑1 (MQ=255)

                                      tcGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGAt                                                                                                        >  1:1916007/1‑148 (MQ=255)

                                      tcGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGAt                                                                                                        >  1:141430/1‑148 (MQ=255)

                                       cGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATc                                                                                                       >  1:2667314/1‑148 (MQ=255)

                                        ggTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCg                                                                                                      <  1:22180/148‑1 (MQ=255)

                                                     tGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATg                                                                                         <  2:2422775/148‑1 (MQ=255)

                                                      gAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATgg                                                                                        >  1:3306727/1‑148 (MQ=255)

                                                       aaaaCGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGc                                                                                       >  2:3172753/1‑148 (MQ=255)

                                                          aCGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAag                                                                                    <  1:1548572/148‑1 (MQ=255)

                                                          aCGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAag                                                                                    >  1:1309597/1‑148 (MQ=255)

                                                           cGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAaga                                                                                   >  1:3515025/1‑148 (MQ=255)

                                                            gTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAagag                                                                                  <  2:3151990/148‑1 (MQ=255)

                                                              tCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGtt                                                                                <  1:2198501/148‑1 (MQ=255)

                                                               cTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGttt                                                                               >  1:70478/1‑148 (MQ=255)

                                                                 ttACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGttttt                                                                             >  2:2536696/1‑148 (MQ=255)

                                                                  tACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTc                                                                            >  2:2825380/1‑148 (MQ=255)

                                                                  tACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTc                                                                            >  2:544221/1‑148 (MQ=255)

                                                                   aCTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCa                                                                           >  2:3299744/1‑148 (MQ=255)

                                                                     tgtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAt                                                                         >  2:2443284/1‑148 (MQ=255)

                                                                     tgtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAt                                                                         >  1:1152896/1‑148 (MQ=255)

                                                                     tgtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAt                                                                         >  1:1646143/1‑148 (MQ=255)

                                                                     tgtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAt                                                                         >  1:3382963/1‑148 (MQ=255)

                                                                       tgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATaa                                                                       >  2:3009742/1‑148 (MQ=255)

                                                                        gATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAAc                                                                      >  1:868757/1‑148 (MQ=255)

                                                                        gATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAAc                                                                      >  2:1364656/1‑148 (MQ=255)

                                                                            gTGCCGCTGACTACAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACcgcg                                                                  <  2:741790/148‑1 (MQ=255)

                                                                               ccGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACt                                                               >  2:1254068/1‑148 (MQ=255)

                                                                                 gCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTgg                                                             <  1:364340/148‑1 (MQ=255)

                                                                                   tGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGcc                                                           >  2:2059601/1‑148 (MQ=255)

                                                                                      cTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGTCCGGt                                                        >  2:839614/1‑148 (MQ=255)

                                                                                      cTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGt                                                        >  1:2166432/1‑148 (MQ=255)

                                                                                          aGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGcc                                                    >  2:698454/1‑148 (MQ=255)

                                                                                          aGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGcc                                                    >  1:3446880/1‑148 (MQ=255)

                                                                                          aGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGcc                                                    <  1:448154/148‑1 (MQ=255)

                                                                                          aGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGcc                                                    <  2:2423063/148‑1 (MQ=255)

                                                                                            gTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTc                                                  >  1:1545779/1‑148 (MQ=255)

                                                                                              gggATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTcgg                                                <  1:1898126/148‑1 (MQ=255)

                                                                                              gggATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTcgg                                                <  1:1899753/148‑1 (MQ=255)

                                                                                               ggATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTcggc                                               >  2:1243825/1‑148 (MQ=255)

                                                                                                 attattGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTcggcgg                                             <  1:165701/148‑1 (MQ=255)

                                                                                                   tattGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTa                                           <  1:1019765/148‑1 (MQ=255)

                                                                                                   tattGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTa                                           <  1:2771664/148‑1 (MQ=255)

                                                                                                    aatGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGTCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCTGCGGTAg                                          >  2:2934280/3‑148 (MQ=255)

                                                                                                     ttGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGc                                         >  2:2681002/1‑148 (MQ=255)

                                                                                                      tGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCg                                        >  1:1271707/1‑148 (MQ=255)

                                                                                                           gATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatcat                                   >  2:1728145/1‑148 (MQ=255)

                                                                                                           gATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatcat                                   <  2:2512512/148‑1 (MQ=255)

                                                                                                           gATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatcat                                   >  2:3050736/1‑148 (MQ=255)

                                                                                                           gATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatcat                                   <  1:484508/148‑1 (MQ=255)

                                                                                                            aTCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatcatc                                  <  2:47190/148‑1 (MQ=255)

                                                                                                             tCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGTCTCGGCGGTAGCGAtcatca                                 >  1:2115279/1‑148 (MQ=255)

                                                                                                             tCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGTCTCGGCGGTAGCGAtcatca                                 >  1:346917/1‑148 (MQ=255)

                                                                                                              cGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATcatcat                                <  1:28451/148‑1 (MQ=255)

                                                                                                               gATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATg                               >  2:1657490/1‑148 (MQ=255)

                                                                                                                  gCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtg                            >  2:397241/1‑148 (MQ=255)

                                                                                                                  gCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtg                            <  1:65528/148‑1 (MQ=255)

                                                                                                                    tGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgct                          >  2:25950/1‑148 (MQ=255)

                                                                                                                    tGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgct                          >  2:1359996/1‑148 (MQ=255)

                                                                                                                       tgtTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgctgct                       >  2:3481117/1‑148 (MQ=255)

                                                                                                                        gtTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgctgctg                      <  2:2766624/148‑1 (MQ=255)

                                                                                                                            aTCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTaa                  <  1:837042/148‑1 (MQ=255)

                                                                                                                            aTCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTaa                  <  2:1320709/148‑1 (MQ=255)

                                                                                                                             tCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAAt                 <  1:2039966/148‑1 (MQ=255)

                                                                                                                             tCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAAt                 <  1:2040397/148‑1 (MQ=255)

                                                                                                                             tCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAAt                 <  2:3373909/148‑1 (MQ=255)

                                                                                                                              cccGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAAtt                <  1:644606/148‑1 (MQ=255)

                                                                                                                                  gCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGc            <  2:1476706/148‑1 (MQ=255)

                                                                                                                                     gtgGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGa         <  2:410145/148‑1 (MQ=255)

                                                                                                                                      tgGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGAt        <  1:1975870/148‑1 (MQ=255)

                                                                                                                                       gggCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATa       <  1:1763276/148‑1 (MQ=255)

                                                                                                                                        ggCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATaa      <  2:2858109/148‑1 (MQ=255)

                                                                                                                                         gCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAAt     >  2:182295/1‑148 (MQ=255)

                                                                                                                                          cGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAAtg    <  2:105101/148‑1 (MQ=255)

                                                                                                                                            aGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAAtgtg  >  1:2574368/1‑148 (MQ=255)

TCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTG  >  CP009273/892213‑892500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCAC  >  CP009273/892210‑892507

GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTT                                                                                                                                                      <  SRR19973065.633429/150‑1 (MQ=60)

GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTT                                                                                                                                                      <  SRR19973065.40043/150‑1 (MQ=60)

GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTT                                                                                                                                                      <  SRR19973065.633386/150‑1 (MQ=60)

GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTT                                                                                                                                                      <  SRR19973065.383332/150‑1 (MQ=60)

   TCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGAT                                                                                                                                                   <  SRR19973065.3291683/150‑1 (MQ=60)

   TCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGAT                                                                                                                                                   <  SRR19973065.3474047/150‑1 (MQ=60)

   TCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGAT                                                                                                                                                   <  SRR19973065.3474193/150‑1 (MQ=60)

    CGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATC                                                                                                                                                  <  SRR19973065.3231477/150‑1 (MQ=60)

     GTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCC                                                                                                                                                 >  SRR19973065.2440953/1‑150 (MQ=60)

     GNATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCC                                                                                                                                                 >  SRR19973065.2840692/1‑150 (MQ=60)

     GNATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCC                                                                                                                                                 >  SRR19973065.2840691/1‑150 (MQ=60)

      TATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCC                                                                                                                                                <  SRR19973065.2535736/150‑1 (MQ=60)

       ATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCG                                                                                                                                               >  SRR19973065.774285/1‑150 (MQ=60)

          GATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAA                                                                                                                                            >  SRR19973065.1988191/1‑150 (MQ=60)

          GATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAA                                                                                                                                            >  SRR19973065.3268491/1‑150 (MQ=60)

          GATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACAGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAA                                                                                                                                            >  SRR19973065.2760818/1‑150 (MQ=60)

           ATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACGAAAGGTGGGCTTATTGCCTGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAAC                                                                                                                                           >  SRR19973065.3078072/1‑150 (MQ=60)

            TTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACT                                                                                                                                          <  SRR19973065.1903444/150‑1 (MQ=60)

            TTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACT                                                                                                                                          <  SRR19973065.3177885/150‑1 (MQ=60)

            TTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACT                                                                                                                                          <  SRR19973065.1902676/150‑1 (MQ=60)

             TATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTG                                                                                                                                         >  SRR19973065.2965694/1‑150 (MQ=60)

              ATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGC                                                                                                                                        >  SRR19973065.830568/1‑150 (MQ=60)

              ATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGC                                                                                                                                        >  SRR19973065.1536593/1‑150 (MQ=60)

               TTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCT                                                                                                                                       <  SRR19973065.1911075/150‑1 (MQ=60)

                  GCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGG                                                                                                                                    <  SRR19973065.3375776/150‑1 (MQ=60)

                     GGTGGAAGGAGCGCTGGATCTCTGTGCACGACCGCTGAAAACGTTCTTTCCTGGGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGCTTCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGGGG                                                                                                                                 <  SRR19973065.968689/150‑1 (MQ=60)

                     GGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGG                                                                                                                                 <  SRR19973065.2733463/150‑1 (MQ=60)

                        GGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACTTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGAGTATTGCAGGATCGATGCTGGTGTTTATCCCGGCTGTGGTCTAGTTTTTGATCCCGGAACTGCTCGGTGGCCC                                                                                                                              >  SRR19973065.3394542/1‑150 (MQ=60)

                           AGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGA                                                                                                                           >  SRR19973065.3044781/1‑150 (MQ=60)

                                CGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCA                                                                                                                      <  SRR19973065.762974/150‑1 (MQ=60)

                                CGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCA                                                                                                                      <  SRR19973065.762978/150‑1 (MQ=60)

                                 GCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCAT                                                                                                                     <  SRR19973065.2324907/150‑1 (MQ=60)

                                 GCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCAT                                                                                                                     <  SRR19973065.2324918/150‑1 (MQ=60)

                                  CTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATC                                                                                                                    >  SRR19973065.1329444/1‑150 (MQ=60)

                                  CTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATC                                                                                                                    <  SRR19973065.1205043/150‑1 (MQ=60)

                                  CTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATC                                                                                                                    <  SRR19973065.1205017/150‑1 (MQ=60)

                                  CTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATC                                                                                                                    >  SRR19973065.857520/1‑150 (MQ=60)

                                  CTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATC                                                                                                                    >  SRR19973065.432954/1‑150 (MQ=60)

                                       TCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGAT                                                                                                               >  SRR19973065.142635/1‑150 (MQ=60)

                                       TCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGAT                                                                                                               >  SRR19973065.1928578/1‑150 (MQ=60)

                                        CTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATC                                                                                                              >  SRR19973065.2683828/1‑150 (MQ=60)

                                           GGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGG                                                                                                           <  SRR19973065.3487263/150‑1 (MQ=60)

                                           GGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGG                                                                                                           <  SRR19973065.22476/150‑1 (MQ=60)

                                                   ACCGCTGAAAACGTTCTGTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCGGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCT                                                                                                   >  SRR19973065.2076175/1‑150 (MQ=60)

                                                     CGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTAT                                                                                                 >  SRR19973065.88219/1‑150 (MQ=60)

                                                       CTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGG                                                                                               >  SRR19973065.3326878/1‑150 (MQ=60)

                                                       CTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTGTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTCTGG                                                                                               >  SRR19973065.968799/1‑150 (MQ=60)

                                                           AAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAG                                                                                           >  SRR19973065.1318242/1‑150 (MQ=60)

                                                            AACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGA                                                                                          >  SRR19973065.2780026/1‑150 (MQ=60)

                                                            AACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGA                                                                                          >  SRR19973065.3536333/1‑150 (MQ=60)

                                                             ACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAG                                                                                         <  SRR19973065.1558819/150‑1 (MQ=60)

                                                              CGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGT                                                                                        >  SRR19973065.2209902/1‑150 (MQ=60)

                                                              CGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGT                                                                                        >  SRR19973065.1924062/1‑150 (MQ=60)

                                                                TTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTT                                                                                      >  SRR19973065.2137676/1‑150 (MQ=60)

                                                                TTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTT                                                                                      >  SRR19973065.70967/1‑150 (MQ=60)

                                                                TTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTT                                                                                      >  SRR19973065.71216/1‑150 (MQ=60)

                                                                TTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTT                                                                                      >  SRR19973065.356251/1‑150 (MQ=60)

                                                                 TCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTT                                                                                     <  SRR19973065.2212579/150‑1 (MQ=60)

                                                                    TTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCA                                                                                  <  SRR19973065.2367084/150‑1 (MQ=60)

                                                                      ACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAT                                                                                >  SRR19973065.1160310/1‑150 (MQ=60)

                                                                      ACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAT                                                                                >  SRR19973065.3403645/1‑150 (MQ=60)

                                                                      ACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAAT                                                                                >  SRR19973065.1657192/1‑150 (MQ=60)

                                                                         GTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAAC                                                                             >  SRR19973065.874325/1‑150 (MQ=60)

                                                                           GATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCG                                                                           <  SRR19973065.2552605/150‑1 (MQ=60)

                                                                            ATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGC                                                                          <  SRR19973065.2228323/150‑1 (MQ=60)

                                                                                    GCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCC                                                                  <  SRR19973065.366892/150‑1 (MQ=60)

                                                                                       GACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGT                                                               >  SRR19973065.401315/1‑150 (MQ=60)

                                                                                       GACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGT                                                               >  SRR19973065.2180351/1‑150 (MQ=60)

                                                                                           AAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCC                                                           >  SRR19973065.3467891/1‑150 (MQ=60)

                                                                                            AAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCT                                                          >  SRR19973065.1675565/1‑150 (MQ=60)

                                                                                            AAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCT                                                          >  SRR19973065.3423662/1‑150 (MQ=60)

                                                                                             AGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTC                                                         <  SRR19973065.451002/150‑1 (MQ=60)

                                                                                             AGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTC                                                         <  SRR19973065.451265/150‑1 (MQ=60)

                                                                                             AGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTC                                                         >  SRR19973065.1556003/1‑150 (MQ=60)

                                                                                             AGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTC                                                         >  SRR19973065.1556850/1‑150 (MQ=60)

                                                                                              GGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCG                                                        <  SRR19973065.1907214/150‑1 (MQ=60)

                                                                                                 GGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCG                                                     <  SRR19973065.1912235/150‑1 (MQ=60)

                                                                                                 GGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCG                                                     <  SRR19973065.1910601/150‑1 (MQ=60)

                                                                                                    ATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTA                                                  <  SRR19973065.166431/150‑1 (MQ=60)

                                                                                                    ATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTA                                                  <  SRR19973065.167055/150‑1 (MQ=60)

                                                                                                      TATTGCCGGATCGTTGCTGGTGTTTATCCCGGCTGTGGGCGTGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGC                                                <  SRR19973065.336012/150‑1 (MQ=60)

                                                                                                      TATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGC                                                <  SRR19973065.1026527/150‑1 (MQ=60)

                                                                                                      TATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGC                                                <  SRR19973065.2788877/150‑1 (MQ=60)

                                                                                                       ATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCG                                               >  SRR19973065.1280098/1‑150 (MQ=60)

                                                                                                             GGATCGTTGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTTTGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATC                                         <  SRR19973065.2962849/150‑1 (MQ=60)

                                                                                                              GATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGTCTCGGCGGTAGCGATCATCA                                        >  SRR19973065.349655/1‑150 (MQ=60)

                                                                                                              GATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGTCTCGGCGGTAGCGATCATCA                                        >  SRR19973065.349344/1‑150 (MQ=60)

                                                                                                              GATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGTCTCGGCGGTAGCGATCATCA                                        >  SRR19973065.2128891/1‑150 (MQ=60)

                                                                                                              GATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCA                                        <  SRR19973065.487766/150‑1 (MQ=60)

                                                                                                              GATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCA                                        <  SRR19973065.487798/150‑1 (MQ=60)

                                                                                                              GATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCA                                        >  SRR19973065.2795034/1‑150 (MQ=60)

                                                                                                                 CGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGT                                     <  SRR19973065.31164/150‑1 (MQ=60)

                                                                                                                 CGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGT                                     <  SRR19973065.28819/150‑1 (MQ=60)

                                                                                                                     GCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCT                                 <  SRR19973065.1280796/150‑1 (MQ=60)

                                                                                                                     GCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCT                                 >  SRR19973065.506170/1‑150 (MQ=60)

                                                                                                                     GCTGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCT                                 <  SRR19973065.66226/150‑1 (MQ=60)

                                                                                                                       TGGTGTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGC                               >  SRR19973065.1268406/1‑150 (MQ=60)

                                                                                                                           GTTTATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCT                           <  SRR19973065.2534549/150‑1 (MQ=60)

                                                                                                                               ATCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATT                       <  SRR19973065.842495/150‑1 (MQ=60)

                                                                                                                                TCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTG                      <  SRR19973065.2053460/150‑1 (MQ=60)

                                                                                                                                TCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTG                      <  SRR19973065.2053436/150‑1 (MQ=60)

                                                                                                                                TCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTG                      <  SRR19973065.2053029/150‑1 (MQ=60)

                                                                                                                                TCCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTG                      <  SRR19973065.2052684/150‑1 (MQ=60)

                                                                                                                                 CCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGT                     <  SRR19973065.648840/150‑1 (MQ=60)

                                                                                                                                 CCCGGCTGTGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGT                     <  SRR19973065.648844/150‑1 (MQ=60)

                                                                                                                                         TGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAA             <  SRR19973065.1988730/150‑1 (MQ=60)

                                                                                                                                         TGGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAA             <  SRR19973065.1628626/150‑1 (MQ=60)

                                                                                                                                          GGGCGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAAT            <  SRR19973065.1774844/150‑1 (MQ=60)

                                                                                                                                             CGAGTTTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTG         >  SRR19973065.2590429/1‑150 (MQ=60)

                                                                                                                                                  TTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCC    <  SRR19973065.443067/150‑1 (MQ=60)

                                                                                                                                                  TTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCC    <  SRR19973065.746022/150‑1 (MQ=60)

                                                                                                                                                  TTTTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCC    <  SRR19973065.443359/150‑1 (MQ=60)

                                                                                                                                                    TTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCAC  <  SRR19973065.1148808/150‑1 (MQ=60)

                                                                                                                                                    TTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCAC  <  SRR19973065.1148774/150‑1 (MQ=60)

GATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAACGTTCTTTACTGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCAC  >  CP009273/892210‑892507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`