Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 994,328 G→T 100% G128V (GGA→GTA)  elfD → putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273994,3280GT98.7% 748.4 / ‑0.8 231G128V (GGA→GTA) elfDputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (135/93);  total (136/95)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

ATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA  >  CP009273/994183‑994472
                                                                                                                                                 |                                                                                                                                                
 ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTaaa                                                                                                                                               >  1:457824/1‑148 (MQ=255)
 ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTaaa                                                                                                                                               <  1:601584/148‑1 (MQ=255)
  taCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAt                                                                                                                                              <  1:768672/148‑1 (MQ=255)
      ccaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAccc                                                                                                                                          >  2:2681028/1‑148 (MQ=255)
       caccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCt                                                                                                                                         >  2:411507/1‑148 (MQ=255)
            cTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGa                                                                                                                                    <  2:1628355/148‑1 (MQ=255)
            cTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGa                                                                                                                                    >  1:1689860/1‑148 (MQ=255)
                ttGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGc                                                                                                                                >  2:338926/1‑148 (MQ=255)
                  gtgCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCca                                                                                                                              <  2:919890/148‑1 (MQ=255)
                    gCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcaca                                                                                                                            <  1:3329075/148‑1 (MQ=255)
                     cTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcacac                                                                                                                           >  1:1056142/1‑148 (MQ=255)
                       gAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACaa                                                                                                                         >  1:61680/1‑148 (MQ=255)
                       gAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACaa                                                                                                                         <  2:1105503/148‑1 (MQ=255)
                          tCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGc                                                                                                                      >  2:1832428/1‑148 (MQ=255)
                             ggCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtc                                                                                                                   <  1:904021/148‑1 (MQ=255)
                               cAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtcat                                                                                                                 <  2:1697813/148‑1 (MQ=255)
                                     agaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACt                                                                                                           >  2:2101764/1‑148 (MQ=255)
                                      gaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACtt                                                                                                          >  1:2595088/1‑148 (MQ=255)
                                       aaTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                         >  1:1866118/1‑148 (MQ=255)
                                        aTCTGTTACGCATAATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGACACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACtttt                                                                                                        <  2:2024422/148‑1 (MQ=255)
                                         tCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTc                                                                                                       <  1:1835998/148‑1 (MQ=255)
                                         tCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTc                                                                                                       >  2:1159734/1‑148 (MQ=255)
                                            gTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTgg                                                                                                    >  2:2624436/1‑148 (MQ=255)
                                                cGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGAc                                                                                                >  1:1238298/1‑148 (MQ=255)
                                                 gCATTATGTACATTGGAGGGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCAAACAAAGCGTCATCAAACTTTTCTGGCGAcc                                                                                               <  2:9622/148‑1 (MQ=255)
                                                    ttATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaa                                                                                            >  2:1780666/1‑148 (MQ=255)
                                                    ttATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaa                                                                                            <  2:1304810/148‑1 (MQ=255)
                                                     tATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaaa                                                                                           <  2:3452256/148‑1 (MQ=255)
                                                     tATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaaa                                                                                           >  1:3166156/1‑148 (MQ=255)
                                                     tATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACACTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaaa                                                                                           >  2:3387638/1‑148 (MQ=255)
                                                      aTGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAg                                                                                          <  1:1080597/148‑1 (MQ=255)
                                                      aTGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAg                                                                                          >  2:384500/1‑148 (MQ=255)
                                                        gTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGt                                                                                        <  2:2310547/148‑1 (MQ=255)
                                                        gTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGt                                                                                        >  1:1743823/1‑148 (MQ=255)
                                                           cATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTa                                                                                     <  2:1056153/148‑1 (MQ=255)
                                                           cATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTa                                                                                     <  2:2835262/148‑1 (MQ=255)
                                                           cATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTa                                                                                     >  2:2021034/1‑148 (MQ=255)
                                                            aTTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAg                                                                                    >  2:1502949/1‑148 (MQ=255)
                                                                gAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTa                                                                                <  1:698032/148‑1 (MQ=255)
                                                                  gcgcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATc                                                                              <  1:2038061/148‑1 (MQ=255)
                                                                   cgcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATcc                                                                             >  1:354175/1‑148 (MQ=255)
                                                                   cgcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATcc                                                                             >  1:1553470/1‑148 (MQ=255)
                                                                        ttGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                        <  2:1875167/148‑1 (MQ=255)
                                                                          ggCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGc                                                                      <  2:457824/148‑1 (MQ=255)
                                                                            cGAAAGACAGAGACACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGa                                                                    <  1:3057156/148‑1 (MQ=255)
                                                                              aaaGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAgg                                                                  <  1:3033474/148‑1 (MQ=255)
                                                                               aaGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGc                                                                 >  1:2171038/1‑148 (MQ=255)
                                                                                aGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCt                                                                >  2:2007645/1‑148 (MQ=255)
                                                                                aGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCt                                                                >  1:267290/1‑148 (MQ=255)
                                                                                 gACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTc                                                               <  1:854975/148‑1 (MQ=255)
                                                                                 gACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTc                                                               >  2:3071160/1‑148 (MQ=255)
                                                                                   cAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCg                                                             >  1:2186947/1‑148 (MQ=255)
                                                                                   cAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCg                                                             >  1:392938/1‑148 (MQ=255)
                                                                                     gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGc                                                           >  1:1144445/1‑148 (MQ=255)
                                                                                      agaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCg                                                          >  2:610381/1‑148 (MQ=255)
                                                                                       gaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGa                                                         >  1:665430/1‑148 (MQ=255)
                                                                                          aCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAAc                                                      >  1:2316941/1‑148 (MQ=255)
                                                                                             cTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGc                                                   >  2:2543096/1‑148 (MQ=255)
                                                                                             cTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGc                                                   >  2:604446/1‑148 (MQ=255)
                                                                                              ttttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCg                                                  <  2:971832/148‑1 (MQ=255)
                                                                                               tttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGt                                                 <  1:2532950/148‑1 (MQ=255)
                                                                                               tttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGt                                                 <  1:35117/148‑1 (MQ=255)
                                                                                                 tcttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTg                                               <  1:1832428/148‑1 (MQ=255)
                                                                                                   ttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCa                                             <  2:1136469/148‑1 (MQ=255)
                                                                                                   ttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCa                                             <  2:1866118/148‑1 (MQ=255)
                                                                                                    tcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCAc                                            <  1:2861841/148‑1 (MQ=255)
                                                                                                         aGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGt                                       >  2:1384468/1‑148 (MQ=255)
                                                                                                         aGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGt                                       <  1:2930261/148‑1 (MQ=255)
                                                                                                           cGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCa                                     >  1:1329278/1‑148 (MQ=255)
                                                                                                            gTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAg                                    <  1:2339753/148‑1 (MQ=255)
                                                                                                            gTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAg                                    <  2:16456/148‑1 (MQ=255)
                                                                                                              aCGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCt                                  <  1:2458881/148‑1 (MQ=255)
                                                                                                               cGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTg                                 >  1:3561865/1‑148 (MQ=255)
                                                                                                                gggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGa                                <  1:1514593/148‑1 (MQ=255)
                                                                                                                   cAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACAt                             >  1:1371550/1‑148 (MQ=255)
                                                                                                                   cAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACAt                             >  1:1443736/1‑148 (MQ=255)
                                                                                                                      tCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGt                          >  2:2921278/1‑148 (MQ=255)
                                                                                                                      tCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGt                          >  2:122365/1‑148 (MQ=255)
                                                                                                                      tCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGt                          >  2:2717643/1‑148 (MQ=255)
                                                                                                                      tCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGt                          >  1:927738/1‑148 (MQ=255)
                                                                                                                       cccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGtt                         >  2:2428447/1‑148 (MQ=255)
                                                                                                                        ccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTa                        >  1:1561721/1‑148 (MQ=255)
                                                                                                                         cTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTAc                       >  1:191758/1‑148 (MQ=255)
                                                                                                                           tCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACgg                     >  1:2802479/1‑148 (MQ=255)
                                                                                                                            caacaaCGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCACAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGt                    <  1:2101764/148‑1 (MQ=255)
                                                                                                                            caacaaCGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGt                    <  1:2147434/148‑1 (MQ=255)
                                                                                                                              acaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCa                  >  1:940891/1‑148 (MQ=255)
                                                                                                                                aacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGt                <  1:1645077/148‑1 (MQ=255)
                                                                                                                                aacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGt                >  2:626050/1‑148 (MQ=255)
                                                                                                                                aacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGt                <  1:3409743/148‑1 (MQ=255)
                                                                                                                                aacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGt                >  2:1733209/1‑148 (MQ=255)
                                                                                                                                aacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCGGGGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGt                >  2:297681/1‑148 (MQ=255)
                                                                                                                                          aaaGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAacac      >  1:2915782/1‑148 (MQ=255)
                                                                                                                                          aaaGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAacac      >  2:2793667/1‑148 (MQ=255)
                                                                                                                                           aaGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACAcc     <  1:777395/148‑1 (MQ=255)
                                                                                                                                            agagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCt    >  2:3186350/1‑148 (MQ=255)
                                                                                                                                            agagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCt    <  1:338881/148‑1 (MQ=255)
                                                                                                                                             gagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCtt   >  2:2272260/1‑148 (MQ=255)
                                                                                                                                             gagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCtt   <  1:2429110/148‑1 (MQ=255)
                                                                                                                                             gagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCtt   <  1:536289/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA  >  CP009273/994183‑994472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATTT  >  CP009273/994182‑994475
                                                                                                                                                  |                                                                                                                                                   
TATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAA                                                                                                                                                  >  SRR19973066.1144611/1‑150 (MQ=60)
TATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAA                                                                                                                                                  >  SRR19973066.461338/1‑150 (MQ=60)
 ATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAT                                                                                                                                                 <  SRR19973066.3021120/150‑1 (MQ=60)
 ATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAT                                                                                                                                                 >  SRR19973066.3314363/1‑150 (MQ=60)
 ATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAT                                                                                                                                                 >  SRR19973066.682554/1‑150 (MQ=60)
  TTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATA                                                                                                                                                <  SRR19973066.605719/150‑1 (MQ=60)
   TACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAC                                                                                                                                               <  SRR19973066.774367/150‑1 (MQ=60)
   TACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAC                                                                                                                                               <  SRR19973066.774259/150‑1 (MQ=60)
      ACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCT                                                                                                                                            >  SRR19973066.437761/1‑150 (MQ=60)
       CCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTG                                                                                                                                           <  SRR19973066.1612911/150‑1 (MQ=60)
           CGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGA                                                                                                                                       >  SRR19973066.1702156/1‑150 (MQ=60)
                    TGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACAC                                                                                                                              <  SRR19973066.1174659/150‑1 (MQ=60)
                    TGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACAC                                                                                                                              >  SRR19973066.1063914/1‑150 (MQ=60)
                    TGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACAC                                                                                                                              >  SRR19973066.2854317/1‑150 (MQ=60)
                    TGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACAC                                                                                                                              >  SRR19973066.1063903/1‑150 (MQ=60)
                     GCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACA                                                                                                                             <  SRR19973066.3351193/150‑1 (MQ=60)
                      CTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAA                                                                                                                            >  SRR19973066.62443/1‑150 (MQ=60)
                      CTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAA                                                                                                                            >  SRR19973066.61710/1‑150 (MQ=60)
                             CGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCA                                                                                                                     <  SRR19973066.899341/150‑1 (MQ=60)
                             CGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAAAAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCA                                                                                                                     <  SRR19973066.907171/150‑1 (MQ=60)
                              GGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCAT                                                                                                                    <  SRR19973066.910400/150‑1 (MQ=60)
                                 AAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAA                                                                                                                 >  SRR19973066.600814/1‑150 (MQ=60)
                                     GAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTT                                                                                                             >  SRR19973066.2612670/1‑150 (MQ=60)
                                     GAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTT                                                                                                             >  SRR19973066.2612666/1‑150 (MQ=60)
                                      AGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTT                                                                                                            >  SRR19973066.1879450/1‑150 (MQ=60)
                                       GAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTT                                                                                                           <  SRR19973066.170410/150‑1 (MQ=60)
                                       GAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTT                                                                                                           <  SRR19973066.171232/150‑1 (MQ=60)
                                          TCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTG                                                                                                        <  SRR19973066.34749/150‑1 (MQ=60)
                                          TCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTG                                                                                                        <  SRR19973066.1849113/150‑1 (MQ=60)
                                           CTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGG                                                                                                       >  SRR19973066.1598594/1‑150 (MQ=60)
                                            TGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGC                                                                                                      >  SRR19973066.606658/1‑150 (MQ=60)
                                               TACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGAC                                                                                                   >  SRR19973066.1247125/1‑150 (MQ=60)
                                                    ATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAA                                                                                              >  SRR19973066.1402026/1‑150 (MQ=60)
                                                    ATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAA                                                                                              >  SRR19973066.2179746/1‑150 (MQ=60)
                                                    ATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAA                                                                                              >  SRR19973066.3187246/1‑150 (MQ=60)
                                                       ATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGT                                                                                           <  SRR19973066.1088490/150‑1 (MQ=60)
                                                       ATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGT                                                                                           >  SRR19973066.1756384/1‑150 (MQ=60)
                                                          TACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTA                                                                                        <  SRR19973066.2502966/150‑1 (MQ=60)
                                                           ACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACTGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAG                                                                                       >  SRR19973066.825976/1‑150 (MQ=60)
                                                               TGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTA                                                                                   <  SRR19973066.3511103/150‑1 (MQ=60)
                                                                 GAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATC                                                                                 <  SRR19973066.703183/150‑1 (MQ=60)
                                                                 GAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATC                                                                                 <  SRR19973066.703231/150‑1 (MQ=60)
                                                                 GAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATC                                                                                 <  SRR19973066.2503048/150‑1 (MQ=60)
                                                                  AGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCC                                                                                >  SRR19973066.358362/1‑150 (MQ=60)
                                                                  AGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCC                                                                                >  SRR19973066.356931/1‑150 (MQ=60)
                                                                  AGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCC                                                                                >  SRR19973066.1564616/1‑150 (MQ=60)
                                                                   GCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCC                                                                               <  SRR19973066.2052266/150‑1 (MQ=60)
                                                                       CGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAG                                                                           >  SRR19973066.3560452/1‑150 (MQ=60)
                                                                       CGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAG                                                                           <  SRR19973066.3165435/150‑1 (MQ=60)
                                                                             CGAAAGACAGAGACACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGG                                                                     <  SRR19973066.3077599/150‑1 (MQ=60)
                                                                              GAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGC                                                                    >  SRR19973066.1560380/1‑150 (MQ=60)
                                                                              GAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGC                                                                    >  SRR19973066.1911881/1‑150 (MQ=60)
                                                                              GAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGC                                                                    >  SRR19973066.2186114/1‑150 (MQ=60)
                                                                               AAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCT                                                                   >  SRR19973066.2080916/1‑150 (MQ=60)
                                                                               AAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCT                                                                   <  SRR19973066.3053745/150‑1 (MQ=60)
                                                                               AAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCT                                                                   >  SRR19973066.269282/1‑150 (MQ=60)
                                                                                  GACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCG                                                                <  SRR19973066.948862/150‑1 (MQ=60)
                                                                                  GACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCG                                                                <  SRR19973066.861150/150‑1 (MQ=60)
                                                                                  GACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCG                                                                >  SRR19973066.396036/1‑150 (MQ=60)
                                                                                  GACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCG                                                                >  SRR19973066.2202122/1‑150 (MQ=60)
                                                                                  GACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCG                                                                >  SRR19973066.2202121/1‑150 (MQ=60)
                                                                                    CAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGC                                                              >  SRR19973066.1152621/1‑150 (MQ=60)
                                                                                     AGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCG                                                             >  SRR19973066.2569870/1‑150 (MQ=60)
                                                                                      GNGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGA                                                            >  SRR19973066.1877921/1‑150 (MQ=60)
                                                                                      GAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGA                                                            >  SRR19973066.670287/1‑150 (MQ=60)
                                                                                         AAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAAC                                                         >  SRR19973066.2332787/1‑150 (MQ=60)
                                                                                         AAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAAC                                                         >  SRR19973066.2332900/1‑150 (MQ=60)
                                                                                         AAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAAC                                                         >  SRR19973066.2332881/1‑150 (MQ=60)
                                                                                                TTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTG                                                  <  SRR19973066.35595/150‑1 (MQ=60)
                                                                                                TTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTG                                                  <  SRR19973066.389113/150‑1 (MQ=60)
                                                                                                TTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTG                                                  <  SRR19973066.2550171/150‑1 (MQ=60)
                                                                                                  TCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTTAATACCCTGAAGATTGCCACACAAAGCTTCATCAAACTTTTCTGGCGACCAATAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCA                                                <  SRR19973066.1845552/150‑1 (MQ=60)
                                                                                                  TCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCA                                                <  SRR19973066.1845515/150‑1 (MQ=60)
                                                                                                   CTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCAC                                               >  SRR19973066.2884298/1‑150 (MQ=60)
                                                                                                   CTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCAC                                               >  SRR19973066.2176733/1‑150 (MQ=60)
                                                                                                     TCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTT                                             >  SRR19973066.1340526/1‑150 (MQ=60)
                                                                                                     TCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTT                                             >  SRR19973066.2868921/1‑150 (MQ=60)
                                                                                                     TCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTT                                             <  SRR19973066.2881057/150‑1 (MQ=60)
                                                                                                          AGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCA                                        <  SRR19973066.3042630/150‑1 (MQ=60)
                                                                                                          AGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCA                                        >  SRR19973066.1338914/1‑150 (MQ=60)
                                                                                                          AGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCA                                        >  SRR19973066.1338895/1‑150 (MQ=60)
                                                                                                          AGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCA                                        <  SRR19973066.2949765/150‑1 (MQ=60)
                                                                                                          AGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCA                                        <  SRR19973066.2949767/150‑1 (MQ=60)
                                                                                                            CGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGC                                      >  SRR19973066.1470724/1‑150 (MQ=60)
                                                                                                            CGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGC                                      >  SRR19973066.3376873/1‑150 (MQ=60)
                                                                                                             GTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCT                                     <  SRR19973066.2355689/150‑1 (MQ=60)
                                                                                                              TACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTG                                    >  SRR19973066.3585486/1‑150 (MQ=60)
                                                                                                              TACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTG                                    >  SRR19973066.3585485/1‑150 (MQ=60)
                                                                                                               ACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGA                                   <  SRR19973066.779402/150‑1 (MQ=60)
                                                                                                               ACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGA                                   <  SRR19973066.2475642/150‑1 (MQ=60)
                                                                                                                 GGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACA                                 <  SRR19973066.1525224/150‑1 (MQ=60)
                                                                                                                  GGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACAT                                >  SRR19973066.1454009/1‑150 (MQ=60)
                                                                                                                  GGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACAT                                >  SRR19973066.2415060/1‑150 (MQ=60)
                                                                                                                  GGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACAT                                >  SRR19973066.1381473/1‑150 (MQ=60)
                                                                                                                     AGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGT                             >  SRR19973066.3206934/1‑150 (MQ=60)
                                                                                                                     AGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGT                             >  SRR19973066.934374/1‑150 (MQ=60)
                                                                                                                     AGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGT                             >  SRR19973066.934451/1‑150 (MQ=60)
                                                                                                                      GTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTT                            <  SRR19973066.183460/150‑1 (MQ=60)
                                                                                                                       TCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTA                           >  SRR19973066.1572964/1‑150 (MQ=60)
                                                                                                                       TCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTA                           >  SRR19973066.1572728/1‑150 (MQ=60)
                                                                                                                        CCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTAC                          >  SRR19973066.1996928/1‑150 (MQ=60)
                                                                                                                        CCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTAC                          >  SRR19973066.193409/1‑150 (MQ=60)
                                                                                                                         CCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACG                         >  SRR19973066.1812513/1‑150 (MQ=60)
                                                                                                                          CTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGG                        >  SRR19973066.3074778/1‑150 (MQ=60)
                                                                                                                          CTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGG                        >  SRR19973066.2821335/1‑150 (MQ=60)
                                                                                                                            TCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTC                      <  SRR19973066.2332362/150‑1 (MQ=60)
                                                                                                                             CAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCACAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCA                     <  SRR19973066.2116239/150‑1 (MQ=60)
                                                                                                                             CAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCA                     >  SRR19973066.947747/1‑150 (MQ=60)
                                                                                                                             CAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCA                     >  SRR19973066.648050/1‑150 (MQ=60)
                                                                                                                             CAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCA                     >  SRR19973066.946250/1‑150 (MQ=60)
                                                                                                                             CAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCA                     <  SRR19973066.2162337/150‑1 (MQ=60)
                                                                                                                             CAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCA                     >  SRR19973066.2162318/1‑150 (MQ=60)
                                                                                                                                 AACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAA                 <  SRR19973066.3432531/150‑1 (MQ=60)
                                                                                                                                 AACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAA                 <  SRR19973066.1657056/150‑1 (MQ=60)
                                                                                                                                         GGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACAC         >  SRR19973066.2935236/1‑150 (MQ=60)
                                                                                                                                           AAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCT       >  SRR19973066.502145/1‑150 (MQ=60)
                                                                                                                                           AAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCT       >  SRR19973066.1319019/1‑150 (MQ=60)
                                                                                                                                            AAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTT      <  SRR19973066.783141/150‑1 (MQ=60)
                                                                                                                                            AAGAGGTAAATACCCTGAAGAGTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTT      <  SRR19973066.1949856/150‑1 (MQ=60)
                                                                                                                                             AGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA     <  SRR19973066.2771492/150‑1 (MQ=60)
                                                                                                                                             AGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA     <  SRR19973066.341488/150‑1 (MQ=60)
                                                                                                                                             AGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA     <  SRR19973066.341534/150‑1 (MQ=60)
                                                                                                                                              GAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTAT    >  SRR19973066.1238448/1‑150 (MQ=60)
                                                                                                                                              GAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTAT    <  SRR19973066.2446423/150‑1 (MQ=60)
                                                                                                                                              GAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTAT    <  SRR19973066.540192/150‑1 (MQ=60)
                                                                                                                                              GAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTAT    <  SRR19973066.2445630/150‑1 (MQ=60)
                                                                                                                                               AGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATT   <  SRR19973066.1485560/150‑1 (MQ=60)
                                                                                                                                                GGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATTT  >  SRR19973066.1934438/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                   
TATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATTT  >  CP009273/994182‑994475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑