Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	994,328	G→T	100%	G128V (GGA→GTA)	elfD →	putative periplasmic pilin chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	994,328	0	G	T	98.7%	789.7 / ‑1.1	242	G128V (GGA→GTA)	elfD	putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand): ref base G (4/0); new base T (121/117); total (125/117)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

TATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATT  >  CP009273/994182‑994474

tattaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTaaa                                                                                                                                                 <  2:3660542/150‑1 (MQ=255)

 attaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAt                                                                                                                                                <  2:82691/150‑1 (MQ=255)

  ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATa                                                                                                                                               >  1:3666313/1‑150 (MQ=255)

  ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATa                                                                                                                                               <  2:608115/150‑1 (MQ=255)

   taCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCCGTCCCTTCAACAACGAAGCGGACAGAGGGAAATAc                                                                                                                                              >  2:3503599/1‑150 (MQ=255)

   taCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAc                                                                                                                                              >  2:422323/1‑150 (MQ=255)

     caccacCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAccc                                                                                                                                            <  2:2098157/150‑1 (MQ=255)

      accaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCt                                                                                                                                           <  2:472036/150‑1 (MQ=255)

      accaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCt                                                                                                                                           >  1:4111908/1‑150 (MQ=255)

      accaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCt                                                                                                                                           >  1:2797638/1‑150 (MQ=255)

          ccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAg                                                                                                                                       <  1:3386564/150‑1 (MQ=255)

          ccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAg                                                                                                                                       >  1:3435823/1‑150 (MQ=255)

           cGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGa                                                                                                                                      >  2:2607282/1‑150 (MQ=255)

           cGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATGGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGa                                                                                                                                      <  1:2093784/150‑1 (MQ=255)

             cTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGAtt                                                                                                                                    >  2:4419945/1‑150 (MQ=255)

              tATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTg                                                                                                                                   <  1:1769908/150‑1 (MQ=255)

                tttGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGcc                                                                                                                                 >  1:2565261/1‑150 (MQ=255)

                  tgtgCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcac                                                                                                                               >  1:978168/1‑150 (MQ=255)

                   gtgCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcaca                                                                                                                              <  2:4267003/150‑1 (MQ=255)

                    tgCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcacac                                                                                                                             >  1:4897176/1‑150 (MQ=255)

                       tGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACaaa                                                                                                                          <  2:3794437/150‑1 (MQ=255)

                       tGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACaaa                                                                                                                          >  2:1407623/1‑150 (MQ=255)

                            ccGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGACAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtc                                                                                                                     >  2:633714/1‑150 (MQ=255)

                             cGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtca                                                                                                                    <  1:4081797/150‑1 (MQ=255)

                                cAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtcatca                                                                                                                 <  1:1065413/150‑1 (MQ=255)

                                 aaaTGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCaa                                                                                                                <  1:961964/150‑1 (MQ=255)

                                   aTGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAAc                                                                                                              <  1:4181931/150‑1 (MQ=255)

                                      agaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCATTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                           <  2:234346/150‑1 (MQ=255)

                                         aTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCt                                                                                                        <  2:2914073/150‑1 (MQ=255)

                                            tGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGc                                                                                                     >  1:122543/1‑150 (MQ=255)

                                             gTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGca                                                                                                    <  1:4584644/150‑2 (MQ=255)

                                               tACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTCCAACAACGAAGCGGAAAGAGGTAGATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGAc                                                                                                  >  1:865793/1‑150 (MQ=255)

                                                 cGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCa                                                                                                >  2:791442/1‑150 (MQ=255)

                                                   cATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaa                                                                                              >  1:3104466/1‑150 (MQ=255)

                                                       aTGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGt                                                                                          >  2:4346933/1‑150 (MQ=255)

                                                        tGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGtt                                                                                         <  1:1708445/150‑1 (MQ=255)

                                                        tGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGtt                                                                                         <  1:4338924/150‑1 (MQ=255)

                                                          tACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTa                                                                                       <  2:2068069/150‑1 (MQ=255)

                                                            cATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGc                                                                                     <  2:1951871/150‑1 (MQ=255)

                                                             aTTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCg                                                                                    <  2:4802124/150‑1 (MQ=255)

                                                             aTTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCg                                                                                    <  1:186402/150‑1 (MQ=255)

                                                              ttGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGt                                                                                   <  2:698067/150‑1 (MQ=255)

                                                                ggAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTAt                                                                                 <  2:3516685/150‑1 (MQ=255)

                                                                 gAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATc                                                                                >  1:4666571/1‑150 (MQ=255)

                                                                   gcgcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATccc                                                                              <  1:3912095/150‑1 (MQ=255)

                                                                    cgcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCt                                                                             >  1:4601360/1‑150 (MQ=255)

                                                                     gcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCtt                                                                            <  2:666581/150‑1 (MQ=255)

                                                                      ccGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAGACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTa                                                                           <  1:3214812/150‑1 (MQ=255)

                                                                       cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                          <  2:1763577/150‑1 (MQ=255)

                                                                       cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                          <  2:2950552/150‑1 (MQ=255)

                                                                       cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                          >  1:2041526/1‑150 (MQ=255)

                                                                       cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                          <  1:2986229/150‑1 (MQ=255)

                                                                        gTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAgg                                                                         >  2:3009136/1‑150 (MQ=255)

                                                                          tGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCg                                                                       <  2:3321939/150‑1 (MQ=255)

                                                                          tGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCg                                                                       >  1:2881302/1‑150 (MQ=255)

                                                                             cGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAgg                                                                    >  2:2550156/1‑150 (MQ=255)

                                                                              gAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGc                                                                   >  2:4158374/1‑150 (MQ=255)

                                                                                aaGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTc                                                                 >  2:2855419/1‑150 (MQ=255)

                                                                                aaGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTc                                                                 <  2:1001040/150‑1 (MQ=255)

                                                                                 ataCAGAGAAACCCTTTTATTCACTAGCTTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGATGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCTATGCtac                                                                <  2:1441918/148‑3 (MQ=255)

                                                                                  gACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGAGTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCg                                                               <  2:4568223/150‑1 (MQ=255)

                                                                                      gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGa                                                           <  2:3205733/150‑1 (MQ=255)

                                                                                      gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAGACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGa                                                           <  2:3368716/150‑1 (MQ=255)

                                                                                        gaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaaa                                                         >  1:1434678/1‑150 (MQ=255)

                                                                                         aaaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAAc                                                        <  2:4256800/150‑1 (MQ=255)

                                                                                                tttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTg                                                 <  2:4280587/150‑1 (MQ=255)

                                                                                                   cttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCAc                                              <  1:5036356/150‑1 (MQ=255)

                                                                                                    ttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCAca                                             <  1:4631264/150‑2 (MQ=255)

                                                                                                     tcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGTCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACtt                                            <  1:671538/150‑1 (MQ=255)

                                                                                                      cACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCCAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTc                                           <  2:1401527/150‑1 (MQ=255)

                                                                                                         tAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTc                                        <  2:3931045/150‑1 (MQ=255)

                                                                                                         tAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTc                                        >  1:102095/1‑150 (MQ=255)

                                                                                                          aGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCa                                       >  2:4978584/1‑150 (MQ=255)

                                                                                                          aGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTAGCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCa                                       <  2:4367488/150‑1 (MQ=255)

                                                                                                            cGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGc                                     >  1:793056/1‑150 (MQ=255)

                                                                                                             gTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCt                                    >  2:1913218/1‑150 (MQ=255)

                                                                                                             gTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCt                                    >  1:1455529/1‑150 (MQ=255)

                                                                                                             gTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCt                                    >  1:384576/1‑150 (MQ=255)

                                                                                                              tACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTg                                   >  1:2849889/1‑150 (MQ=255)

                                                                                                              tACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTg                                   >  2:1785621/1‑150 (MQ=255)

                                                                                                                 gggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACa                                >  1:2357964/1‑150 (MQ=255)

                                                                                                                  ggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACAt                               <  2:2433977/150‑1 (MQ=255)

                                                                                                                          cTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACgg                       <  1:2607282/150‑1 (MQ=255)

                                                                                                                             caacaaCGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCa                    <  2:2799299/150‑1 (MQ=255)

                                                                                                                             caacaaCGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCa                    <  1:44462/150‑1 (MQ=255)

                                                                                                                             caacaaCGAAGCGGAAAGAGGTAAATACCCTGAAGAGTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCa                    <  2:1524984/150‑1 (MQ=255)

                                                                                                                              aacaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAg                   <  1:3097567/150‑1 (MQ=255)

                                                                                                                              aacaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAg                   >  1:4256707/1‑150 (MQ=255)

                                                                                                                              aacaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAg                   >  1:3686623/1‑150 (MQ=255)

                                                                                                                               acaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGt                  >  1:3277074/1‑150 (MQ=255)

                                                                                                                               acaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGt                  >  1:755966/1‑150 (MQ=255)

                                                                                                                                caacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTa                 <  2:128479/150‑1 (MQ=255)

                                                                                                                                caacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTa                 >  2:580440/1‑150 (MQ=255)

                                                                                                                                  acGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAAc               >  1:2246518/1‑150 (MQ=255)

                                                                                                                                     aaGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCa            >  2:1892251/1‑150 (MQ=255)

                                                                                                                                      aGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCaa           >  2:221315/1‑150 (MQ=255)

                                                                                                                                      aGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCaa           >  2:3410571/1‑150 (MQ=255)

                                                                                                                                        cGGAAAGATGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGTCATGGTTACGGTCAGTAACCCAaca         <  1:4375432/150‑1 (MQ=255)

                                                                                                                                             agagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTa    <  1:4816270/150‑1 (MQ=255)

                                                                                                                                             agagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTa    >  1:3739829/1‑150 (MQ=255)

TATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATT  >  CP009273/994182‑994474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATT  >  CP009273/994180‑994474

ATTATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTA                                                                                                                                                   <  SRR19973063.4408055/150‑1 (MQ=60)

ATTATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTA                                                                                                                                                   <  SRR19973063.4407931/150‑1 (MQ=60)

    TTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATA                                                                                                                                               >  SRR19973063.3677898/1‑150 (MQ=60)

    TTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATA                                                                                                                                               >  SRR19973063.3678063/1‑150 (MQ=60)

        ACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCT                                                                                                                                           >  SRR19973063.2806630/1‑150 (MQ=60)

        ACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCT                                                                                                                                           >  SRR19973063.4125078/1‑150 (MQ=60)

        ACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCT                                                                                                                                           <  SRR19973063.4267271/150‑1 (MQ=60)

         CCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTG                                                                                                                                          >  SRR19973063.295534/1‑150 (MQ=60)

         CCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTG                                                                                                                                          >  SRR19973063.3943597/1‑150 (MQ=60)

          CACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGA                                                                                                                                         <  SRR19973063.3755457/150‑1 (MQ=60)

            CCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAG                                                                                                                                       <  SRR19973063.3397353/150‑1 (MQ=60)

            CCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAG                                                                                                                                       >  SRR19973063.3446751/1‑150 (MQ=60)

             CGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGA                                                                                                                                      <  SRR19973063.943605/150‑1 (MQ=60)

             CGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGA                                                                                                                                      <  SRR19973063.2100401/150‑1 (MQ=60)

             CGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGA                                                                                                                                      >  SRR19973063.709117/1‑150 (MQ=60)

             CGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATGGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGA                                                                                                                                      <  SRR19973063.2100325/150‑1 (MQ=60)

                TATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTG                                                                                                                                   <  SRR19973063.1775358/150‑1 (MQ=60)

                  TTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCC                                                                                                                                 >  SRR19973063.2573435/1‑150 (MQ=60)

                  TTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCC                                                                                                                                 >  SRR19973063.4368156/1‑150 (MQ=60)

                  TTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCC                                                                                                                                 >  SRR19973063.4368110/1‑150 (MQ=60)

                    TGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCAC                                                                                                                               >  SRR19973063.981117/1‑150 (MQ=60)

                      TGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACAC                                                                                                                             >  SRR19973063.4912981/1‑150 (MQ=60)

                       GCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACA                                                                                                                            <  SRR19973063.4092842/150‑1 (MQ=60)

                        CTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAA                                                                                                                           >  SRR19973063.2536482/1‑150 (MQ=60)

                           AATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGC                                                                                                                        >  SRR19973063.1862759/1‑150 (MQ=60)

                           AATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGC                                                                                                                        >  SRR19973063.1691993/1‑150 (MQ=60)

                            ATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCG                                                                                                                       <  SRR19973063.3854442/150‑1 (MQ=60)

                             TCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGT                                                                                                                      >  SRR19973063.602566/1‑150 (MQ=60)

                               CGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCA                                                                                                                    <  SRR19973063.4094866/150‑1 (MQ=60)

                                 GCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATC                                                                                                                  <  SRR19973063.2969356/150‑1 (MQ=60)

                                  CAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCA                                                                                                                 <  SRR19973063.4855730/150‑1 (MQ=60)

                                  CAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCA                                                                                                                 <  SRR19973063.1068647/150‑1 (MQ=60)

                                   AAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAA                                                                                                                <  SRR19973063.964859/150‑1 (MQ=60)

                                     ATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAAC                                                                                                              <  SRR19973063.661095/150‑1 (MQ=60)

                                     ATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAAC                                                                                                              <  SRR19973063.4195285/150‑1 (MQ=60)

                                          AATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTC                                                                                                         <  SRR19973063.3569775/150‑1 (MQ=60)

                                             CTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGG                                                                                                      <  SRR19973063.975221/150‑1 (MQ=60)

                                              TGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGC                                                                                                     <  SRR19973063.2526310/150‑1 (MQ=60)

                                              TGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGC                                                                                                     >  SRR19973063.122944/1‑150 (MQ=60)

                                               GTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCG                                                                                                    <  SRR19973063.75949/150‑1 (MQ=60)

                                               GTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCA                                                                                                    <  SRR19973063.4599319/150‑1 (MQ=60)

                                                 TACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTCCAACAACGAAGCGGAAAGAGGTAGATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGAC                                                                                                  >  SRR19973063.868284/1‑150 (MQ=60)

                                                     CATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAA                                                                                              >  SRR19973063.3114456/1‑150 (MQ=60)

                                                       TTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAG                                                                                            >  SRR19973063.4510677/1‑150 (MQ=60)

                                                        TATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGG                                                                                           >  SRR19973063.3337983/1‑150 (MQ=60)

                                                          TGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTT                                                                                         <  SRR19973063.1713648/150‑1 (MQ=60)

                                                          TGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTT                                                                                         <  SRR19973063.4352756/150‑1 (MQ=60)

                                                            TACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTA                                                                                       >  SRR19973063.4933127/1‑150 (MQ=60)

                                                             ACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAG                                                                                      <  SRR19973063.1755607/150‑1 (MQ=60)

                                                               ATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCG                                                                                    >  SRR19973063.3698393/1‑150 (MQ=60)

                                                               ATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCG                                                                                    <  SRR19973063.186978/150‑1 (MQ=60)

                                                                  TGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTAT                                                                                 >  SRR19973063.4153152/1‑150 (MQ=60)

                                                                   GAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATC                                                                                >  SRR19973063.1064524/1‑150 (MQ=60)

                                                                   GAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATC                                                                                >  SRR19973063.4681456/1‑150 (MQ=60)

                                                                     GCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCC                                                                              <  SRR19973063.3924572/150‑1 (MQ=60)

                                                                      CGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCT                                                                             >  SRR19973063.4616082/1‑150 (MQ=60)

                                                                      CGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCT                                                                             >  SRR19973063.4616280/1‑150 (MQ=60)

                                                                        CCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAGACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTA                                                                           <  SRR19973063.3225147/150‑1 (MQ=60)

                                                                         CGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAG                                                                          <  SRR19973063.2995761/150‑1 (MQ=60)

                                                                         CGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAG                                                                          >  SRR19973063.2047941/1‑150 (MQ=60)

                                                                         CGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAG                                                                          >  SRR19973063.2047906/1‑150 (MQ=60)

                                                                         CGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAG                                                                          >  SRR19973063.1112948/1‑150 (MQ=60)

                                                                          GTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGG                                                                         >  SRR19973063.858723/1‑150 (MQ=60)

                                                                            TGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCG                                                                       <  SRR19973063.4327221/150‑1 (MQ=60)

                                                                            TGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCG                                                                       >  SRR19973063.2890516/1‑150 (MQ=60)

                                                                               CGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGG                                                                    >  SRR19973063.3860809/1‑150 (MQ=60)

                                                                                  AAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTC                                                                 <  SRR19973063.4678237/150‑1 (MQ=60)

                                                                                    GACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCG                                                               <  SRR19973063.977455/150‑1 (MQ=60)

                                                                                          GAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAA                                                         >  SRR19973063.3422400/1‑150 (MQ=60)

                                                                                          GAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAA                                                         >  SRR19973063.1439055/1‑150 (MQ=60)

                                                                                            AACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACT                                                       >  SRR19973063.995650/1‑150 (MQ=60)

                                                                                              CCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCTAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGC                                                     <  SRR19973063.1916592/150‑1 (MQ=60)

                                                                                              CCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGC                                                     <  SRR19973063.1916561/150‑1 (MQ=60)

                                                                                              CCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGC                                                     <  SRR19973063.4681589/150‑1 (MQ=60)

                                                                                              CCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGC                                                     <  SRR19973063.2838562/150‑1 (MQ=60)

                                                                                              CCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGC                                                     <  SRR19973063.4681352/150‑1 (MQ=60)

                                                                                               CCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCG                                                    <  SRR19973063.4611052/150‑1 (MQ=60)

                                                                                                  TTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTG                                                 >  SRR19973063.3345325/1‑150 (MQ=60)

                                                                                                  TTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTG                                                 >  SRR19973063.2702116/1‑150 (MQ=60)

                                                                                                   TTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGC                                                >  SRR19973063.325742/1‑150 (MQ=60)

                                                                                                    TCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCA                                               >  SRR19973063.3121088/1‑150 (MQ=60)

                                                                                                     CTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCAC                                              <  SRR19973063.5052590/150‑1 (MQ=60)

                                                                                                      TTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACT                                             >  SRR19973063.1274057/1‑150 (MQ=60)

                                                                                                      TTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACA                                             <  SRR19973063.4646066/150‑1 (MQ=60)

                                                                                                       TCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGTCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTT                                            <  SRR19973063.673501/150‑1 (MQ=60)

                                                                                                        CACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTC                                           >  SRR19973063.4113415/1‑150 (MQ=60)

                                                                                                           TAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTC                                        >  SRR19973063.102445/1‑150 (MQ=60)

                                                                                                            AGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCA                                       >  SRR19973063.3113434/1‑150 (MQ=60)

                                                                                                              CGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGC                                     >  SRR19973063.795348/1‑150 (MQ=60)

                                                                                                               GTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCT                                    >  SRR19973063.385675/1‑150 (MQ=60)

                                                                                                               GTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCT                                    >  SRR19973063.1459900/1‑150 (MQ=60)

                                                                                                               GTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCT                                    >  SRR19973063.1459967/1‑150 (MQ=60)

                                                                                                                TACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTG                                   >  SRR19973063.2859194/1‑150 (MQ=60)

                                                                                                                TACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTG                                   >  SRR19973063.2859029/1‑150 (MQ=60)

                                                                                                                TACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTG                                   >  SRR19973063.4884659/1‑150 (MQ=60)

                                                                                                                  CGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAAACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCTTCAGCTGAC                                 >  SRR19973063.1646116/1‑150 (MQ=60)

                                                                                                                   GGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACA                                >  SRR19973063.2365503/1‑150 (MQ=60)

                                                                                                                   GGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACA                                >  SRR19973063.2365511/1‑150 (MQ=60)

                                                                                                                       AGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGT                            >  SRR19973063.312929/1‑150 (MQ=60)

                                                                                                                           CCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACG                        >  SRR19973063.845681/1‑150 (MQ=60)

                                                                                                                            CTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGG                       <  SRR19973063.2615567/150‑1 (MQ=60)

                                                                                                                            CTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGG                       <  SRR19973063.2615457/150‑1 (MQ=60)

                                                                                                                               CAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCA                    <  SRR19973063.44627/150‑1 (MQ=60)

                                                                                                                               CAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCA                    <  SRR19973063.2118275/150‑1 (MQ=60)

                                                                                                                                AACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAG                   >  SRR19973063.3698432/1‑150 (MQ=60)

                                                                                                                                AACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAG                   >  SRR19973063.4270257/1‑150 (MQ=60)

                                                                                                                                AACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAG                   <  SRR19973063.1435666/150‑1 (MQ=60)

                                                                                                                                AACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAG                   >  SRR19973063.4142646/1‑150 (MQ=60)

                                                                                                                                AACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAG                   >  SRR19973063.3698428/1‑150 (MQ=60)

                                                                                                                                AACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAG                   <  SRR19973063.3107533/150‑1 (MQ=60)

                                                                                                                                 ACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGT                  >  SRR19973063.758169/1‑150 (MQ=60)

                                                                                                                                 ACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGT                  >  SRR19973063.3287595/1‑150 (MQ=60)

                                                                                                                                    ACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAAC               >  SRR19973063.2253662/1‑150 (MQ=60)

                                                                                                                                     CGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACC              >  SRR19973063.4584534/1‑150 (MQ=60)

                                                                                                                                       AAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCA            <  SRR19973063.4410767/150‑1 (MQ=60)

                                                                                                                                          CGGAAAGATGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGTCATGGTTACGGTCAGTAACCCAACA         <  SRR19973063.4389438/150‑1 (MQ=60)

                                                                                                                                           GGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACAC        >  SRR19973063.3088905/1‑150 (MQ=60)

                                                                                                                                               AGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA    >  SRR19973063.3751778/1‑150 (MQ=60)

                                                                                                                                               AGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA    <  SRR19973063.4831679/150‑1 (MQ=60)

                                                                                                                                                 AGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATT  >  SRR19973063.2847031/1‑150 (MQ=60)

ATTATTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTATT  >  CP009273/994180‑994474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`