Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,139,402 C→A 94.8% R141L (CGC→CTC)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,139,4020CA94.8% 614.6 / 22.8 210R141L (CGC→CTC) rnefused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base C (5/6);  new base A (93/106);  total (98/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAAC  >  CP009273/1139257‑1139545
                                                                                                                                                 |                                                                                                                                               
  tCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGgagaga                                                                                                                                             >  1:2378391/1‑148 (MQ=255)
     gACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAAt                                                                                                                                          <  1:2237658/148‑1 (MQ=255)
     gACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAAt                                                                                                                                          <  1:1539027/148‑1 (MQ=255)
       cGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGc                                                                                                                                        <  1:345056/148‑1 (MQ=255)
         gAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCa                                                                                                                                      >  2:150692/1‑148 (MQ=255)
         gAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCa                                                                                                                                      >  1:2182214/1‑148 (MQ=255)
           aGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAcc                                                                                                                                    <  2:331946/148‑1 (MQ=255)
             cTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcg                                                                                                                                  >  2:1863753/1‑148 (MQ=255)
                 aaTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgcgcg                                                                                                                              <  1:2792992/148‑1 (MQ=255)
                  aTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgcgcgc                                                                                                                             <  1:288676/148‑1 (MQ=255)
                  aTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgcgcgc                                                                                                                             <  1:3135275/148‑1 (MQ=255)
                      cATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGgt                                                                                                                         >  1:807850/1‑148 (MQ=255)
                      cATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGgt                                                                                                                         <  2:1219514/148‑1 (MQ=255)
                          gCAGCGCCTCAGCAGATTTGCCGACGCCAGTGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGgttgtt                                                                                                                     <  1:600789/148‑1 (MQ=255)
                           cAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTc                                                                                                                    <  2:2774591/148‑1 (MQ=255)
                                ccTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAt                                                                                                               <  2:1434817/148‑1 (MQ=255)
                                  tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCa                                                                                                             >  2:52351/1‑148 (MQ=255)
                                  tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCa                                                                                                             >  1:814895/1‑148 (MQ=255)
                                     gcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGaa                                                                                                          <  2:1033666/148‑1 (MQ=255)
                                      cagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAc                                                                                                         >  1:258282/1‑148 (MQ=255)
                                      cagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAc                                                                                                         >  2:3009589/1‑148 (MQ=255)
                                        gATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCa                                                                                                       >  2:3169056/1‑148 (MQ=255)
                                         aTTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAg                                                                                                      >  2:1947937/1‑148 (MQ=255)
                                           gtGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGAt                                                                                                    <  1:2260167/147‑1 (MQ=255)
                                             gCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAg                                                                                                  <  1:3300220/148‑1 (MQ=255)
                                             gCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAg                                                                                                  <  1:1794487/148‑1 (MQ=255)
                                                 aCGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGTCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAc                                                                                              >  2:3507299/1‑148 (MQ=255)
                                                  cGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAcc                                                                                             <  2:782077/148‑1 (MQ=255)
                                                  cGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAcc                                                                                             <  2:488902/148‑1 (MQ=255)
                                                  cGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAcc                                                                                             >  1:1781173/1‑148 (MQ=255)
                                                     cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGc                                                                                          <  2:3233593/148‑1 (MQ=255)
                                                      aGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGcc                                                                                         <  1:3302998/148‑1 (MQ=255)
                                                      aGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGcc                                                                                         <  1:1881595/148‑1 (MQ=255)
                                                       gCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCa                                                                                        >  1:3310015/1‑148 (MQ=255)
                                                         ggTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGa                                                                                      <  2:194041/148‑1 (MQ=255)
                                                         ggTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGa                                                                                      <  2:2526877/148‑1 (MQ=255)
                                                             cgcACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGa                                                                                  <  1:1566106/148‑1 (MQ=255)
                                                             cgcACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGa                                                                                  >  1:927980/1‑148 (MQ=255)
                                                             cgcACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGa                                                                                  >  1:2067154/1‑148 (MQ=255)
                                                                aCGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATaa                                                                               <  2:3430050/148‑1 (MQ=255)
                                                                aCGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATaa                                                                               <  1:2308063/148‑1 (MQ=255)
                                                                  gATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAg                                                                             <  1:2086430/148‑1 (MQ=255)
                                                                   aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAgg                                                                            <  1:3513847/148‑1 (MQ=255)
                                                                   aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAgg                                                                            <  2:738084/148‑1 (MQ=255)
                                                                   aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAgg                                                                            <  1:3321082/148‑1 (MQ=255)
                                                                     aaGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAggtg                                                                          >  1:3284740/1‑148 (MQ=255)
                                                                      aGCCGCATGCCTTCCGTCAGTTCAAGGCTTGCCAGTGCTTCTTTTATTTCGGTACGGTCGTCGCCTTCGATACTGAGAGAACTGCCACCCGCGCGCGTGTTGTTCTGCATCAGAACCAGTTAGCTACCCGCCAGACTGATTAAggtgg                                                                         <  2:1851923/148‑1 (MQ=255)
                                                                      aGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAggtgg                                                                         <  2:2530970/148‑1 (MQ=255)
                                                                      aGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAggtgg                                                                         >  2:516724/1‑148 (MQ=255)
                                                                       gCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAggtggt                                                                        <  2:158211/148‑1 (MQ=255)
                                                                         cccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTa                                                                      <  2:2066794/148‑1 (MQ=255)
                                                                            aTGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATg                                                                   >  2:2200346/1‑148 (MQ=255)
                                                                                 ttCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCcgcg                                                              <  1:865393/148‑1 (MQ=255)
                                                                                  tCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCgcgc                                                             <  2:1729032/148‑1 (MQ=255)
                                                                                   ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGcc                                                            <  1:2672566/148‑1 (MQ=255)
                                                                                      gCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCttt                                                         >  2:729511/1‑148 (MQ=255)
                                                                                       cAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttg                                                        <  1:286346/148‑1 (MQ=255)
                                                                                          ttCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgttg                                                     <  2:258353/148‑1 (MQ=255)
                                                                                          ttCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgttg                                                     >  1:1295840/1‑148 (MQ=255)
                                                                                           tCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGc                                                    >  2:1049094/1‑148 (MQ=255)
                                                                                             aaGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCcg                                                  >  1:1172609/1‑148 (MQ=255)
                                                                                              aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCcgc                                                 >  1:2377132/1‑148 (MQ=255)
                                                                                               ggCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCcgcg                                                >  2:3230656/1‑148 (MQ=255)
                                                                                                gCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCgcgc                                               >  2:634709/1‑148 (MQ=255)
                                                                                                gCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCgcgc                                               >  1:1753692/1‑148 (MQ=255)
                                                                                                   tGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGctct                                            <  1:1040446/148‑1 (MQ=255)
                                                                                                    gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtctt                                           <  2:1243989/148‑1 (MQ=255)
                                                                                                    gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtctt                                           <  1:2508844/148‑1 (MQ=255)
                                                                                                     ccAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttc                                          <  1:3243929/148‑1 (MQ=255)
                                                                                                     ccAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttc                                          >  2:2388800/1‑148 (MQ=255)
                                                                                                      cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                         >  2:1544676/1‑148 (MQ=255)
                                                                                                        gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCttt                                       <  1:1430225/148‑1 (MQ=255)
                                                                                                          gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                     >  2:2175733/1‑148 (MQ=255)
                                                                                                          gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                     >  1:3326800/1‑148 (MQ=255)
                                                                                                          gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                     >  2:3047804/1‑148 (MQ=255)
                                                                                                          gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGACATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                     <  1:1355260/148‑1 (MQ=255)
                                                                                                            ttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCg                                   >  2:581463/1‑148 (MQ=255)
                                                                                                              cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGAt                                 <  2:880868/148‑1 (MQ=255)
                                                                                                                tttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCt                               >  2:2807037/1‑148 (MQ=255)
                                                                                                                 ttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTg                              <  1:426085/148‑1 (MQ=255)
                                                                                                                   aaTTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGaa                            <  2:809853/148‑1 (MQ=255)
                                                                                                                    aTTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAAc                           >  1:2974545/1‑148 (MQ=255)
                                                                                                                     ttCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACa                          <  1:256640/148‑1 (MQ=255)
                                                                                                                      tCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACaa                         <  2:843379/148‑1 (MQ=255)
                                                                                                                      tCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACaa                         <  1:258540/148‑1 (MQ=255)
                                                                                                                      tCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACaa                         <  1:693575/148‑1 (MQ=255)
                                                                                                                         gTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGa                      <  1:84863/148‑1 (MQ=255)
                                                                                                                          tACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCATAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGAc                     <  1:522303/148‑1 (MQ=255)
                                                                                                                           aCGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACt                    <  2:2377799/148‑1 (MQ=255)
                                                                                                                             ggTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTc                  >  1:1232410/1‑148 (MQ=255)
                                                                                                                              gtcgtcGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTcc                 <  2:287101/148‑1 (MQ=255)
                                                                                                                               tcgtcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCt                >  2:2627120/1‑148 (MQ=255)
                                                                                                                                cgtcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTg               >  2:1828468/1‑148 (MQ=255)
                                                                                                                                  tcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGAc             <  1:1766472/148‑1 (MQ=255)
                                                                                                                                  tcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGAc             <  2:1096934/148‑1 (MQ=255)
                                                                                                                                     ccTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCtt          >  2:2523450/1‑148 (MQ=255)
                                                                                                                                      cTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTc         >  2:2038346/1‑148 (MQ=255)
                                                                                                                                       ttCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCa        >  2:761063/1‑148 (MQ=255)
                                                                                                                                        tCGATACGGAGATAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCAc       >  1:2405637/1‑148 (MQ=255)
                                                                                                                                        tCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCAc       >  1:1705403/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAAC  >  CP009273/1139257‑1139545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCT  >  CP009273/1139253‑1139551
                                                                                                                                                     |                                                                                                                                                     
AGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGA                                                                                                                                                       >  SRR19973066.787849/1‑150 (MQ=60)
AGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGA                                                                                                                                                       >  SRR19973066.1480447/1‑150 (MQ=60)
    TTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGA                                                                                                                                                   >  SRR19973066.2393851/1‑150 (MQ=60)
    TTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGA                                                                                                                                                   >  SRR19973066.2394446/1‑150 (MQ=60)
     TTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAC                                                                                                                                                  >  SRR19973066.3337235/1‑150 (MQ=60)
         GACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGC                                                                                                                                              <  SRR19973066.2253089/150‑1 (MQ=60)
         GACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGC                                                                                                                                              <  SRR19973066.1550019/150‑1 (MQ=60)
           CGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCA                                                                                                                                            >  SRR19973066.2197359/1‑150 (MQ=60)
           CGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCA                                                                                                                                            <  SRR19973066.347733/150‑1 (MQ=60)
                GCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGC                                                                                                                                       >  SRR19973066.2401598/1‑150 (MQ=60)
                    AAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGC                                                                                                                                   >  SRR19973066.2490670/1‑150 (MQ=60)
                     AATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCG                                                                                                                                  >  SRR19973066.1336418/1‑150 (MQ=60)
                     AATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCG                                                                                                                                  <  SRR19973066.2811787/150‑1 (MQ=60)
                      ATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGG                                                                                                                                 <  SRR19973066.290750/150‑1 (MQ=60)
                      ATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGG                                                                                                                                 <  SRR19973066.290106/150‑1 (MQ=60)
                      ATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGG                                                                                                                                 <  SRR19973066.3156201/150‑1 (MQ=60)
                      ATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGG                                                                                                                                 >  SRR19973066.3326023/1‑150 (MQ=60)
                        CCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGT                                                                                                                               >  SRR19973066.813781/1‑150 (MQ=60)
                              GCAGCGCCTCAGCAGATTTGCCGACGCCAGTGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCG                                                                                                                         <  SRR19973066.604922/150‑1 (MQ=60)
                                AGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGC                                                                                                                       >  SRR19973066.979801/1‑150 (MQ=60)
                                  CGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAT                                                                                                                     >  SRR19973066.2811975/1‑150 (MQ=60)
                                    CCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCA                                                                                                                   >  SRR19973066.820867/1‑150 (MQ=60)
                                     CTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAG                                                                                                                  >  SRR19973066.618873/1‑150 (MQ=60)
                                      TCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGA                                                                                                                 <  SRR19973066.2652872/150‑1 (MQ=60)
                                        AGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGACATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAC                                                                                                               >  SRR19973066.260230/1‑150 (MQ=60)
                                        AGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAC                                                                                                               >  SRR19973066.260301/1‑150 (MQ=60)
                                        AGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAC                                                                                                               >  SRR19973066.819942/1‑150 (MQ=60)
                                        AGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAC                                                                                                               >  SRR19973066.849502/1‑150 (MQ=60)
                                           AGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAG                                                                                                            >  SRR19973066.1561628/1‑150 (MQ=60)
                                               GTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAG                                                                                                        <  SRR19973066.2275735/150‑1 (MQ=60)
                                                 GCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCT                                                                                                      <  SRR19973066.1807254/150‑1 (MQ=60)
                                                 GCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCT                                                                                                      <  SRR19973066.3322040/150‑1 (MQ=60)
                                                    GACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACC                                                                                                   >  SRR19973066.1793901/1‑150 (MQ=60)
                                                    GACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACC                                                                                                   >  SRR19973066.1794259/1‑150 (MQ=60)
                                                      CGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCG                                                                                                 <  SRR19973066.1135117/150‑1 (MQ=60)
                                                      CGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCG                                                                                                 >  SRR19973066.2961753/1‑150 (MQ=60)
                                                         CAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCA                                                                                              >  SRR19973066.3331968/1‑150 (MQ=60)
                                                          AGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAG                                                                                             <  SRR19973066.1895026/150‑1 (MQ=60)
                                                          AGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAG                                                                                             <  SRR19973066.3324638/150‑1 (MQ=60)
                                                          AGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAG                                                                                             <  SRR19973066.3324640/150‑1 (MQ=60)
                                                          AGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAG                                                                                             <  SRR19973066.3324847/150‑1 (MQ=60)
                                                               TGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGA                                                                                        >  SRR19973066.2081911/1‑150 (MQ=60)
                                                               TGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGA                                                                                        >  SRR19973066.2081466/1‑150 (MQ=60)
                                                               TGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGA                                                                                        >  SRR19973066.934694/1‑150 (MQ=60)
                                                                 CGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATA                                                                                      <  SRR19973066.1577387/150‑1 (MQ=60)
                                                                   CACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAA                                                                                    >  SRR19973066.482536/1‑150 (MQ=60)
                                                                    ACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAG                                                                                   <  SRR19973066.2323867/150‑1 (MQ=60)
                                                                      GATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGT                                                                                 <  SRR19973066.2100821/150‑1 (MQ=60)
                                                                      GATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGT                                                                                 <  SRR19973066.2262015/150‑1 (MQ=60)
                                                                       ATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTG                                                                                <  SRR19973066.3537253/150‑1 (MQ=60)
                                                                       ATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTG                                                                                >  SRR19973066.3306368/1‑150 (MQ=60)
                                                                       ATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTG                                                                                <  SRR19973066.2595718/150‑1 (MQ=60)
                                                                       ATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTG                                                                                <  SRR19973066.3343141/150‑1 (MQ=60)
                                                                        TAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGG                                                                               >  SRR19973066.2404568/1‑150 (MQ=60)
                                                                        TAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGG                                                                               >  SRR19973066.603568/1‑150 (MQ=60)
                                                                             CCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAAT                                                                          >  SRR19973066.1605569/1‑150 (MQ=60)
                                                                             CCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAAT                                                                          >  SRR19973066.1752758/1‑150 (MQ=60)
                                                                                    CTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGC                                                                   >  SRR19973066.912642/1‑150 (MQ=60)
                                                                                     TTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCC                                                                  <  SRR19973066.871610/150‑1 (MQ=60)
                                                                                       CCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTT                                                                <  SRR19973066.2690423/150‑1 (MQ=60)
                                                                                        CGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTT                                                               <  SRR19973066.856048/150‑1 (MQ=60)
                                                                                        CGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTT                                                               >  SRR19973066.2358520/1‑150 (MQ=60)
                                                                                          GCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGT                                                             >  SRR19973066.3209458/1‑150 (MQ=60)
                                                                                           CAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTT                                                            <  SRR19973066.288412/150‑1 (MQ=60)
                                                                                           CAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTT                                                            <  SRR19973066.337582/150‑1 (MQ=60)
                                                                                            AGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTG                                                           >  SRR19973066.1305228/1‑150 (MQ=60)
                                                                                             GTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGTAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGC                                                          <  SRR19973066.1582131/150‑1 (MQ=60)
                                                                                             GTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGC                                                          >  SRR19973066.2294814/1‑150 (MQ=60)
                                                                                             GTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCGTTGTTGC                                                          >  SRR19973066.3047804/1‑150 (MQ=60)
                                                                                              TTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCC                                                         <  SRR19973066.1290823/150‑1 (MQ=60)
                                                                                               TCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCG                                                        >  SRR19973066.1180883/1‑150 (MQ=60)
                                                                                                CAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGC                                                       >  SRR19973066.2393182/1‑150 (MQ=60)
                                                                                                 AAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCG                                                      <  SRR19973066.3294854/150‑1 (MQ=60)
                                                                                                  AGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGC                                                     >  SRR19973066.2130139/1‑150 (MQ=60)
                                                                                                  AGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGC                                                     >  SRR19973066.1766300/1‑150 (MQ=60)
                                                                                                       TGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTC                                                <  SRR19973066.1048101/150‑1 (MQ=60)
                                                                                                        GCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCT                                               <  SRR19973066.1772215/150‑1 (MQ=60)
                                                                                                        GCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCT                                               <  SRR19973066.2526091/150‑1 (MQ=60)
                                                                                                        GCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCT                                               <  SRR19973066.3546167/150‑1 (MQ=60)
                                                                                                        GCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCT                                               <  SRR19973066.2525922/150‑1 (MQ=60)
                                                                                                         CCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTT                                              >  SRR19973066.2736757/1‑150 (MQ=60)
                                                                                                         CCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTT                                              <  SRR19973066.3265321/150‑1 (MQ=60)
                                                                                                          CAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTT                                             >  SRR19973066.1901376/1‑150 (MQ=60)
                                                                                                            GTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAT                                           >  SRR19973066.3348902/1‑150 (MQ=60)
                                                                                                            GTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAT                                           <  SRR19973066.1440437/150‑1 (MQ=60)
                                                                                                              GCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGACATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCG                                         <  SRR19973066.1365074/150‑1 (MQ=60)
                                                                                                                   TTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTG                                    >  SRR19973066.3497802/1‑150 (MQ=60)
                                                                                                                   TTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTG                                    <  SRR19973066.3343403/150‑1 (MQ=60)
                                                                                                                     TTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAA                                  <  SRR19973066.429389/150‑1 (MQ=60)
                                                                                                                     TTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAA                                  <  SRR19973066.429537/150‑1 (MQ=60)
                                                                                                                     TTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAA                                  <  SRR19973066.1848433/150‑1 (MQ=60)
                                                                                                                      TAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAAC                                 >  SRR19973066.2994204/1‑150 (MQ=60)
                                                                                                                         TTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAAT                              <  SRR19973066.258586/150‑1 (MQ=60)
                                                                                                                          TCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATG                             <  SRR19973066.260489/150‑1 (MQ=60)
                                                                                                                          TCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATG                             <  SRR19973066.698689/150‑1 (MQ=60)
                                                                                                                             GTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACT                          <  SRR19973066.85807/150‑1 (MQ=60)
                                                                                                                              TACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCATAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTT                         <  SRR19973066.526138/150‑1 (MQ=60)
                                                                                                                               ACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTC                        >  SRR19973066.1241167/1‑150 (MQ=60)
                                                                                                                                CGGTCGTCGCCTTCGATACGGCGAGAAAAGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCC                       >  SRR19973066.720235/1‑150 (MQ=60)
                                                                                                                                 GGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCT                      <  SRR19973066.3310305/150‑1 (MQ=60)
                                                                                                                                  GTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTG                     >  SRR19973066.2844531/1‑150 (MQ=60)
                                                                                                                                    CGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGAC                   <  SRR19973066.1548233/150‑1 (MQ=60)
                                                                                                                                    CGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGAC                   <  SRR19973066.1403429/150‑1 (MQ=60)
                                                                                                                                      TCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCT                 <  SRR19973066.1779493/150‑1 (MQ=60)
                                                                                                                                      TCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCT                 <  SRR19973066.1225844/150‑1 (MQ=60)
                                                                                                                                      TCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCT                 <  SRR19973066.1779138/150‑1 (MQ=60)
                                                                                                                                       CGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTT                <  SRR19973066.2201584/150‑1 (MQ=60)
                                                                                                                                          CTTCGATACGGAGATAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCAC             >  SRR19973066.2421901/1‑150 (MQ=60)
                                                                                                                                          CTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCAC             >  SRR19973066.1717781/1‑150 (MQ=60)
                                                                                                                                            TCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGC           >  SRR19973066.898446/1‑150 (MQ=60)
                                                                                                                                               ATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATATCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCGTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTACTGACCTTCACGCAAC        >  SRR19973066.2093915/1‑150 (MQ=60)
                                                                                                                                                  CGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACA     >  SRR19973066.3174771/1‑150 (MQ=60)
                                                                                                                                                     AGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCT  >  SRR19973066.2073051/1‑150 (MQ=60)
                                                                                                                                                     AGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCT  >  SRR19973066.2073047/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCT  >  CP009273/1139253‑1139551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑