Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,224,292 G→A 100% D8N (GAT→AAT)  ycgN → UPF0153 family cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,224,2920GA99.5% 649.1 / ‑4.4 199D8N (GAT→AAT) ycgNUPF0153 family cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (104/93);  total (104/95)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

CTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGT  >  CP009273/1224147‑1224435
                                                                                                                                                 |                                                                                                                                               
  gACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGt                                                                                                                                             <  2:2602654/148‑1 (MQ=255)
  gACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGt                                                                                                                                             >  1:1690194/1‑148 (MQ=255)
    cAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTAc                                                                                                                                           <  2:2431564/148‑1 (MQ=255)
       tCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCACTGTACCtt                                                                                                                                        >  2:2273490/1‑148 (MQ=255)
       tCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCtt                                                                                                                                        >  2:1775550/1‑148 (MQ=255)
       tCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCtt                                                                                                                                        >  1:2409182/1‑148 (MQ=255)
       tCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCtt                                                                                                                                        >  1:2441248/1‑148 (MQ=255)
        cACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCttt                                                                                                                                       <  1:1868715/148‑1 (MQ=255)
        cACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCttt                                                                                                                                       <  2:1549320/148‑1 (MQ=255)
              cGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCa                                                                                                                                 >  2:672056/1‑148 (MQ=255)
                aTGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCaaa                                                                                                                               >  1:515656/1‑148 (MQ=255)
                 tGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAg                                                                                                                              <  1:634558/148‑1 (MQ=255)
                 tGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAg                                                                                                                              <  1:1605559/148‑1 (MQ=255)
                 tGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAg                                                                                                                              >  2:2998175/1‑148 (MQ=255)
                  gggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGt                                                                                                                             >  1:1181216/1‑148 (MQ=255)
                  gggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGt                                                                                                                             >  1:2149019/1‑148 (MQ=255)
                   ggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGTTGAGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCATTCTGGCAAAGTa                                                                                                                            >  1:1034486/1‑148 (MQ=255)
                   ggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTa                                                                                                                            >  2:1778259/1‑148 (MQ=255)
                    gctttCTTTGACTACTCGCTTTTTGTATTACTTTTTGCCGCCTGATAGCGGAGGGAAAACTTGCATCGCTGTGCCAGACTGGTTATTAGGTGCGTTTTTACGTAATTGCGGAACACCTGATGAGCTATGTACCTTTCTGGCAAAGTaa                                                                                                                           <  2:704888/145‑1 (MQ=255)
                    gCATTCTTTGACCACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaa                                                                                                                           <  2:704705/148‑1 (MQ=255)
                     cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                          <  1:1021531/148‑1 (MQ=255)
                     cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                          >  1:1659820/1‑148 (MQ=255)
                     cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                          <  1:2856194/148‑1 (MQ=255)
                     cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                          <  1:2802306/148‑1 (MQ=255)
                      aTTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaaa                                                                                                                         <  1:1439775/148‑1 (MQ=255)
                       ttCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAc                                                                                                                        <  1:577338/148‑1 (MQ=255)
                        tCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAcc                                                                                                                       <  2:72360/148‑1 (MQ=255)
                        tCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAcc                                                                                                                       >  2:2681963/1‑148 (MQ=255)
                          tttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCt                                                                                                                     <  1:1916210/148‑1 (MQ=255)
                          tttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCt                                                                                                                     <  1:3002812/148‑1 (MQ=255)
                              aCAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                 >  1:1917561/1‑148 (MQ=255)
                                 aCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGaa                                                                                                              >  2:1643504/1‑148 (MQ=255)
                                   tCGCTTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAAt                                                                                                            >  2:429156/1‑148 (MQ=255)
                                    cgcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATg                                                                                                           >  1:1973312/1‑148 (MQ=255)
                                    cgcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATg                                                                                                           <  2:603360/148‑1 (MQ=255)
                                      cgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAg                                                                                                         <  1:2516338/148‑1 (MQ=255)
                                      cgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAg                                                                                                         <  2:167949/148‑1 (MQ=255)
                                         tttGTAATACTTTTTGCCGCCTGAACGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTAGAAGGTGCGTTTAAACGTAATGGCGGAACACCTGATGAGCAATGTCCCTTTCTGGCAAAGGAAAACCCTGGACGAAATGAGCGa                                                                                                      >  2:3368949/1‑148 (MQ=255)
                                         tttGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTCTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGa                                                                                                      <  2:2387431/148‑1 (MQ=255)
                                               aTACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGa                                                                                                <  2:1693784/148‑1 (MQ=255)
                                                tACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATACGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGaa                                                                                               <  2:881002/148‑1 (MQ=255)
                                                tACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGaa                                                                                               >  2:1824269/1‑148 (MQ=255)
                                                    ttttGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATggg                                                                                           <  2:3121953/148‑1 (MQ=255)
                                                    ttttGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATggg                                                                                           >  1:2082965/1‑148 (MQ=255)
                                                      ttGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAg                                                                                         <  1:766240/148‑1 (MQ=255)
                                                            ccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtg                                                                                   <  2:32121/148‑1 (MQ=255)
                                                            ccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtg                                                                                   >  2:1205764/1‑148 (MQ=255)
                                                            ccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtg                                                                                   <  2:32335/148‑1 (MQ=255)
                                                                aaaGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTggtgtg                                                                               >  2:929413/1‑148 (MQ=255)
                                                                 aaGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtgtgt                                                                              >  1:2783969/1‑147 (MQ=255)
                                                                  aGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGAt                                                                             >  2:2855612/1‑148 (MQ=255)
                                                                    cggcggCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATgg                                                                           <  1:284763/148‑1 (MQ=255)
                                                                        ggctataCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTTATGGCTGAACACCTGATGAGCAATGTTCCTTTCTGTCAATGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGc                                                                       <  2:2788406/142‑1 (MQ=255)
                                                                        ggcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGc                                                                       <  2:1805383/148‑1 (MQ=255)
                                                                             aaCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCa                                                                  >  2:1845796/1‑148 (MQ=255)
                                                                                 tGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAgtgt                                                              <  2:2758543/148‑1 (MQ=255)
                                                                                   cATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTCACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTg                                                            <  2:2083307/148‑1 (MQ=255)
                                                                                   cATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTg                                                            >  1:2258776/1‑148 (MQ=255)
                                                                                     tCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGcc                                                          >  2:2150589/1‑148 (MQ=255)
                                                                                        cTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGc                                                       >  2:792441/1‑148 (MQ=255)
                                                                                          gtgCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCAt                                                     >  2:3521225/1‑148 (MQ=255)
                                                                                          gtgCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGAGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCAt                                                     <  1:3070518/148‑1 (MQ=255)
                                                                                          gtgCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGAGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCAt                                                     <  1:1295371/148‑1 (MQ=255)
                                                                                                 aCTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTga                                              <  1:1402872/148‑1 (MQ=255)
                                                                                                 aCTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTga                                              <  1:283513/148‑1 (MQ=255)
                                                                                                    ggTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatgg                                           >  2:2459428/1‑148 (MQ=255)
                                                                                                     gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatgga                                          >  2:908649/1‑148 (MQ=255)
                                                                                                     gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatgga                                          >  2:972321/1‑148 (MQ=255)
                                                                                                       tataAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggatg                                        >  2:3170885/1‑148 (MQ=255)
                                                                                                            ggTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGac                                   >  1:884529/1‑148 (MQ=255)
                                                                                                             gTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGaca                                  <  1:990281/148‑1 (MQ=255)
                                                                                                             gTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGAGGGTTGCGGTCAGTGTTGGCTGCATAAACTGATGGATGAAGaca                                  >  2:902475/1‑148 (MQ=255)
                                                                                                              tGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGACTGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGacac                                 >  2:1610462/1‑148 (MQ=255)
                                                                                                               gCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTTCGGTCAGTGTTGCCTGCATAAACTGATGGATGAATACAcc                                >  1:381731/1‑148 (MQ=255)
                                                                                                                cGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTTTTGGGTTCGGTCAGTGTTGCCTGCAAAAACAGATGGATGTATACACcg                               >  1:2761348/1‑148 (MQ=255)
                                                                                                                  ttttAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACcgac                             >  1:486289/1‑148 (MQ=255)
                                                                                                                   tttAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACcgacg                            >  1:3530078/1‑148 (MQ=255)
                                                                                                                    ttAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACcgacga                           <  2:165096/148‑1 (MQ=255)
                                                                                                                     tAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGaa                          >  1:2905628/1‑148 (MQ=255)
                                                                                                                      aaCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGaaa                         <  1:3170885/148‑1 (MQ=255)
                                                                                                                      aaCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGaaa                         <  2:2846007/148‑1 (MQ=255)
                                                                                                                       aCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAAt                        >  1:2583980/1‑148 (MQ=255)
                                                                                                                       aCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAAt                        >  2:2070985/1‑148 (MQ=255)
                                                                                                                        cGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCCGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATc                       >  2:336016/1‑148 (MQ=255)
                                                                                                                        cGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATc                       >  1:413513/1‑148 (MQ=255)
                                                                                                                         gTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCt                      <  1:1352877/148‑1 (MQ=255)
                                                                                                                          tAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGGGATGGTTGCGGTCAGTGTTGCCTGCAGAAACTGATGGATGAAGACACCGACGAAATCTa                     >  1:1308511/1‑148 (MQ=255)
                                                                                                                            aTGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACt                   <  2:2441248/148‑1 (MQ=255)
                                                                                                                            aTGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACt                   >  2:1371014/1‑148 (MQ=255)
                                                                                                                             tGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACtt                  >  2:52864/1‑148 (MQ=255)
                                                                                                                             tGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACtt                  >  2:3426429/1‑148 (MQ=255)
                                                                                                                                cGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCAc               >  2:396693/1‑148 (MQ=255)
                                                                                                                                  gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATTGTTGCGGTCAGTGTTGCCTGCATAAACAGATGGATTAATACAACGACGAAATATACttaaata             >  1:478028/1‑143 (MQ=255)
                                                                                                                                  gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTa             <  1:3464393/148‑1 (MQ=255)
                                                                                                                                  gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTa             <  1:1844989/148‑1 (MQ=255)
                                                                                                                                  gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTa             <  1:1319458/148‑1 (MQ=255)
                                                                                                                                   aaCACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTaa            >  1:3244086/1‑148 (MQ=255)
                                                                                                                                     cacCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACg          >  1:180231/1‑148 (MQ=255)
                                                                                                                                        cTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCg       <  2:1349458/148‑1 (MQ=255)
                                                                                                                                          gatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATTAAGACACCGACGAAATCTACTTCACTAACGTCGcc     >  1:2221114/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGT  >  CP009273/1224147‑1224435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAG  >  CP009273/1224145‑1224441
                                                                                                                                                   |                                                                                                                                                     
AGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAAT                                                                                                                                                     >  SRR19973066.2280285/1‑150 (MQ=60)
  CTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGT                                                                                                                                                   >  SRR19973066.1702493/1‑150 (MQ=60)
      CAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCT                                                                                                                                               >  SRR19973066.2168997/1‑150 (MQ=60)
       AGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTT                                                                                                                                              >  SRR19973066.1569861/1‑150 (MQ=60)
       AGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTT                                                                                                                                              >  SRR19973066.2425490/1‑150 (MQ=60)
       AGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTT                                                                                                                                              >  SRR19973066.2457875/1‑150 (MQ=60)
        GTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTT                                                                                                                                             >  SRR19973066.1359240/1‑150 (MQ=60)
          CACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCT                                                                                                                                           <  SRR19973066.1882064/150‑1 (MQ=60)
          CACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCT                                                                                                                                           >  SRR19973066.2865129/1‑150 (MQ=60)
                CGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAA                                                                                                                                     >  SRR19973066.519459/1‑150 (MQ=60)
                CGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAA                                                                                                                                     >  SRR19973066.519450/1‑150 (MQ=60)
                  ATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGT                                                                                                                                   >  SRR19973066.2163936/1‑150 (MQ=60)
                  ATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGT                                                                                                                                   >  SRR19973066.1189518/1‑150 (MQ=60)
                   TGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGTTGAGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCATTCTGGCAAAGTA                                                                                                                                  >  SRR19973066.1042100/1‑150 (MQ=60)
                   TGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTA                                                                                                                                  <  SRR19973066.2801594/150‑1 (MQ=60)
                   TGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTA                                                                                                                                  <  SRR19973066.639125/150‑1 (MQ=60)
                   TGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTA                                                                                                                                  <  SRR19973066.1617209/150‑1 (MQ=60)
                   TGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTA                                                                                                                                  >  SRR19973066.3254431/1‑150 (MQ=60)
                     GGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAA                                                                                                                                >  SRR19973066.1671916/1‑150 (MQ=60)
                       CATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAC                                                                                                                              <  SRR19973066.1029048/150‑1 (MQ=60)
                       CATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAC                                                                                                                              <  SRR19973066.1087815/150‑1 (MQ=60)
                       CATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAC                                                                                                                              <  SRR19973066.2875380/150‑1 (MQ=60)
                       CATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAC                                                                                                                              <  SRR19973066.2821161/150‑1 (MQ=60)
                        ATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACC                                                                                                                             <  SRR19973066.1450025/150‑1 (MQ=60)
                         TTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCC                                                                                                                            <  SRR19973066.581398/150‑1 (MQ=60)
                            TTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGG                                                                                                                         <  SRR19973066.3022961/150‑1 (MQ=60)
                            TTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGG                                                                                                                         <  SRR19973066.1929848/150‑1 (MQ=60)
                            TTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGG                                                                                                                         <  SRR19973066.3022775/150‑1 (MQ=60)
                              TGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAC                                                                                                                       >  SRR19973066.1930535/1‑150 (MQ=60)
                              TGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAC                                                                                                                       >  SRR19973066.1931208/1‑150 (MQ=60)
                              TGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAC                                                                                                                       >  SRR19973066.2066098/1‑150 (MQ=60)
                              TGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGGTTTAACGTAATGTCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAC                                                                                                                       >  SRR19973066.1932462/1‑150 (MQ=60)
                                    CTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATG                                                                                                                 >  SRR19973066.1987256/1‑150 (MQ=60)
                                    CTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATG                                                                                                                 >  SRR19973066.2127625/1‑150 (MQ=60)
                                        CGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCG                                                                                                             <  SRR19973066.2533456/150‑1 (MQ=60)
                                                AATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAA                                                                                                     >  SRR19973066.409922/1‑150 (MQ=60)
                                                 ATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAAT                                                                                                    <  SRR19973066.258466/150‑1 (MQ=60)
                                                    CTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGG                                                                                                 >  SRR19973066.2097337/1‑150 (MQ=60)
                                                    CTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGG                                                                                                 <  SRR19973066.832731/150‑1 (MQ=60)
                                                        TTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTC                                                                                             <  SRR19973066.771916/150‑1 (MQ=60)
                                                         TGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCG                                                                                            <  SRR19973066.3558035/150‑1 (MQ=60)
                                                         TGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCG                                                                                            <  SRR19973066.3008968/150‑1 (MQ=60)
                                                          GCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGT                                                                                           <  SRR19973066.3110471/150‑1 (MQ=60)
                                                          GCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGT                                                                                           <  SRR19973066.1323970/150‑1 (MQ=60)
                                                                 GAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGT                                                                                    >  SRR19973066.2802713/1‑150 (MQ=60)
                                                                 GAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGA                                                                                    >  SRR19973066.2803145/1‑150 (MQ=60)
                                                                      CGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTT                                                                               <  SRR19973066.286820/150‑1 (MQ=60)
                                                                      CGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTT                                                                               <  SRR19973066.287249/150‑1 (MQ=60)
                                                                             AAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCA                                                                        <  SRR19973066.1442015/150‑1 (MQ=60)
                                                                                  TTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTT                                                                   <  SRR19973066.676980/150‑1 (MQ=60)
                                                                                   TGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTG                                                                  >  SRR19973066.2274336/1‑150 (MQ=60)
                                                                                     CATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCC                                                                <  SRR19973066.3276148/150‑1 (MQ=60)
                                                                                            GTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGAGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAA                                                         <  SRR19973066.1305093/150‑1 (MQ=60)
                                                                                            GTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGAGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAA                                                         <  SRR19973066.3091048/150‑1 (MQ=60)
                                                                                            GTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGAGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAA                                                         <  SRR19973066.1304754/150‑1 (MQ=60)
                                                                                                   ACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATG                                                  <  SRR19973066.284011/150‑1 (MQ=60)
                                                                                                   ACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATG                                                  <  SRR19973066.285566/150‑1 (MQ=60)
                                                                                                   ACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATG                                                  <  SRR19973066.1412972/150‑1 (MQ=60)
                                                                                                     TGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGTCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCTGTCAGTGTTGCCTGCATAAACTGATGGA                                                <  SRR19973066.2255747/150‑1 (MQ=60)
                                                                                                     TGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTTCGGTCAGTGTTGCCTGCATAAACACATGGA                                                >  SRR19973066.1916467/1‑150 (MQ=60)
                                                                                                     TGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGA                                                <  SRR19973066.1879290/150‑1 (MQ=60)
                                                                                                        TTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGA                                             >  SRR19973066.2473769/1‑150 (MQ=60)
                                                                                                          ATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAG                                           <  SRR19973066.553653/150‑1 (MQ=60)
                                                                                                            AAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGAC                                         >  SRR19973066.890981/1‑150 (MQ=60)
                                                                                                            AAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGAC                                         >  SRR19973066.890820/1‑150 (MQ=60)
                                                                                                            AAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGAC                                         >  SRR19973066.1390914/1‑150 (MQ=60)
                                                                                                              GGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACAC                                       <  SRR19973066.1659073/150‑1 (MQ=60)
                                                                                                               GTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTTCGGTCAGTGTTGCCTGCATAAACTGATGGATGAATACACC                                      >  SRR19973066.384745/1‑150 (MQ=60)
                                                                                                               GTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTTCGGTCAGTGTTGCCTGCAAAAACTGATGGATGAAGACACC                                      >  SRR19973066.1857283/1‑150 (MQ=60)
                                                                                                               GTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACC                                      <  SRR19973066.997585/150‑1 (MQ=60)
                                                                                                                TGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTTTTGGGTTCGGTCAGTGTTGCCTGCAAAAACAGATGGATGTATACACCG                                     >  SRR19973066.2779942/1‑150 (MQ=60)
                                                                                                                  CGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGAC                                   >  SRR19973066.489940/1‑150 (MQ=60)
                                                                                                                  CGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGAC                                   >  SRR19973066.1737356/1‑150 (MQ=60)
                                                                                                                   GTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACG                                  >  SRR19973066.3553559/1‑150 (MQ=60)
                                                                                                                     TTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAA                                >  SRR19973066.2925032/1‑150 (MQ=60)
                                                                                                                       TAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAAT                              >  SRR19973066.2601827/1‑150 (MQ=60)
                                                                                                                       TAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAAT                              >  SRR19973066.2601494/1‑150 (MQ=60)
                                                                                                                        AACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATC                             <  SRR19973066.3192002/150‑1 (MQ=60)
                                                                                                                        AACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATC                             <  SRR19973066.3529078/150‑1 (MQ=60)
                                                                                                                        AACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATC                             >  SRR19973066.416744/1‑150 (MQ=60)
                                                                                                                        AACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCCACGAAAAC                             >  SRR19973066.63772/1‑150 (MQ=60)
                                                                                                                          CGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTA                           <  SRR19973066.1208204/150‑1 (MQ=60)
                                                                                                                          CGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTA                           <  SRR19973066.2455960/150‑1 (MQ=60)
                                                                                                                          CGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGGGATGGTTGCGGTCAGTGTTGCCTGCAGAAACTGATGGATGAAGACACCGACGAAATCTA                           >  SRR19973066.1318006/1‑150 (MQ=60)
                                                                                                                           GTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTAC                          <  SRR19973066.1362677/150‑1 (MQ=60)
                                                                                                                            TAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTTCGGTCAGTGTTGGCTGCATAAACTGATGGATGAAGACACCGACGAAATCTAAT                         >  SRR19973066.1680863/1‑150 (MQ=60)
                                                                                                                                  CGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATTGTTGCGGTCAGTGTTGCCTGCATAAACAGATGGATTAATACAACGACGAAATATACTTaaata                   >  SRR19973066.481632/1‑145 (MQ=60)
                                                                                                                                  CGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATTGATGAATACACCCACGAAATCTAATTCACTA                   >  SRR19973066.481986/1‑150 (MQ=60)
                                                                                                                                  CGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTA                   >  SRR19973066.481622/1‑150 (MQ=60)
                                                                                                                                  CGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTA                   >  SRR19973066.2907302/1‑150 (MQ=60)
                                                                                                                                   GGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAA                  >  SRR19973066.3265480/1‑150 (MQ=60)
                                                                                                                                    GAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAAC                 <  SRR19973066.1858180/150‑1 (MQ=60)
                                                                                                                                    GAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAAC                 <  SRR19973066.3487550/150‑1 (MQ=60)
                                                                                                                                    GAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAAC                 <  SRR19973066.1329021/150‑1 (MQ=60)
                                                                                                                                     AACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACG                >  SRR19973066.181808/1‑150 (MQ=60)
                                                                                                                                       CACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGTTGCCTGCATAAAATGATGGATTAAGACACCGACGAAATATACTTCACAAACGTC              >  SRR19973066.205465/1‑150 (MQ=60)
                                                                                                                                          CTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATTAAGACACCGACGAAATCTACTTCACTAACGTCGCC           >  SRR19973066.2236478/1‑150 (MQ=60)
                                                                                                                                               GAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACAAACGTCGCCTGTCG      >  SRR19973066.2194323/1‑150 (MQ=60)
                                                                                                                                                AGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGGTGGTTGCGGTCAGTGTTGCCTGCATAAAATGATGGATGTAGACACCGCCGAAATCTACATCACTAACGTCGCCTGTCGC     >  SRR19973066.2126778/1‑150 (MQ=60)
                                                                                                                                                  CAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCA   <  SRR19973066.3544668/150‑1 (MQ=60)
                                                                                                                                                   AATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGGTGGTTGCGGTCAGTGTTGCCTGGCTAAAATTATGGATGAAGACACCGACGAAATCTACTTCACAAACGTCGCCTGTCGCCAG  >  SRR19973066.2484732/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAG  >  CP009273/1224145‑1224441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑