Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,224,292 G→A 94.3% D8N (GAT→AAT)  ycgN → UPF0153 family cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,224,2920GA94.3% 897.6 / 31.9 264D8N (GAT→AAT) ycgNUPF0153 family cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base G (5/10);  new base A (105/143);  total (110/154)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 264 total aligned reads displayed.

AGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGC  >  CP009273/1224145‑1224438
                                                                                                                                                   |                                                                                                                                                  
 gCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATg                                                                                                                                                 <  2:890295/150‑1 (MQ=255)
   tGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTa                                                                                                                                               <  2:3233024/150‑1 (MQ=255)
     aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTAcc                                                                                                                                             >  1:3870064/1‑150 (MQ=255)
     aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTAcc                                                                                                                                             <  2:455856/150‑1 (MQ=255)
      cAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCt                                                                                                                                            >  2:698111/1‑150 (MQ=255)
       aGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCtt                                                                                                                                           <  1:3847210/150‑1 (MQ=255)
         tCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTc                                                                                                                                         <  1:793452/150‑1 (MQ=255)
          cACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCt                                                                                                                                        <  2:4166578/150‑1 (MQ=255)
           aCTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTg                                                                                                                                       >  2:3981610/1‑150 (MQ=255)
            cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTgg                                                                                                                                      <  1:2519975/150‑1 (MQ=255)
            cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTgg                                                                                                                                      <  1:126652/150‑1 (MQ=255)
                cGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCaaa                                                                                                                                  <  2:2699278/150‑1 (MQ=255)
                 gATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAg                                                                                                                                 >  1:2264525/1‑150 (MQ=255)
                 gATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAg                                                                                                                                 >  1:994167/1‑150 (MQ=255)
                  aTGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGt                                                                                                                                >  2:785909/1‑150 (MQ=255)
                   tGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTa                                                                                                                               <  2:3089833/150‑1 (MQ=255)
                    gggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaa                                                                                                                              >  2:349230/1‑150 (MQ=255)
                      gCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTaaaa                                                                                                                            <  1:2697648/150‑1 (MQ=255)
                       cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAc                                                                                                                           <  2:4095499/150‑1 (MQ=255)
                       cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAc                                                                                                                           <  2:4095472/150‑1 (MQ=255)
                       cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAc                                                                                                                           >  2:405177/1‑150 (MQ=255)
                        aTTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAcc                                                                                                                          <  1:2655655/150‑1 (MQ=255)
                          tCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCt                                                                                                                        <  2:1010353/150‑1 (MQ=255)
                           cTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTg                                                                                                                       >  2:1521620/1‑150 (MQ=255)
                            tttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTgg                                                                                                                      <  2:3044515/150‑1 (MQ=255)
                              tGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                    >  2:888272/1‑150 (MQ=255)
                              tGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                    <  1:537272/150‑1 (MQ=255)
                               gACAACTCGCTTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACg                                                                                                                   <  1:1613137/150‑1 (MQ=255)
                               gACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACg                                                                                                                   >  2:2457088/1‑150 (MQ=255)
                                aCAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGa                                                                                                                  >  1:4772070/1‑150 (MQ=255)
                                  aaCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGaaa                                                                                                                <  1:1132107/150‑1 (MQ=255)
                                    cTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATg                                                                                                              <  2:2736554/150‑1 (MQ=255)
                                      cgcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAg                                                                                                            >  1:3310992/1‑150 (MQ=255)
                                      cgcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTAGACGAAATGAg                                                                                                            <  1:1038554/150‑1 (MQ=255)
                                        cgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCg                                                                                                          >  1:1906001/1‑150 (MQ=255)
                                        cgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCg                                                                                                          <  2:1906001/150‑1 (MQ=255)
                                         gTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGa                                                                                                         >  2:4104507/1‑150 (MQ=255)
                                         gTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGa                                                                                                         >  1:602513/1‑150 (MQ=255)
                                           tttGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATg                                                                                                       <  1:350102/150‑1 (MQ=255)
                                             tGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCg                                                                                                     >  2:1176625/1‑150 (MQ=255)
                                               tAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGa                                                                                                   >  1:417628/1‑150 (MQ=255)
                                                  tACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATg                                                                                                <  1:3670573/150‑1 (MQ=255)
                                                   aCTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATgg                                                                                               >  2:125682/1‑150 (MQ=255)
                                                   aCTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATgg                                                                                               <  1:4259916/150‑1 (MQ=255)
                                                    cTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATggg                                                                                              >  2:814856/1‑150 (MQ=255)
                                                    cTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATggg                                                                                              >  2:540393/1‑150 (MQ=255)
                                                          gccgccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGt                                                                                        <  2:3622140/150‑1 (MQ=255)
                                                            cgccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCACTGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtg                                                                                      >  1:3607141/1‑150 (MQ=255)
                                                            cgccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGttt                                                                                      >  1:4045268/1‑149 (MQ=255)
                                                             gccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgt                                                                                     <  2:3888474/150‑1 (MQ=255)
                                                              ccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCgtggtg                                                                                    >  1:2002911/1‑150 (MQ=255)
                                                              ccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtg                                                                                    >  1:2576444/1‑150 (MQ=255)
                                                              ccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtg                                                                                    >  2:1653813/1‑150 (MQ=255)
                                                                tGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtgtg                                                                                  >  1:3744839/1‑150 (MQ=255)
                                                                   aaGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATg                                                                               <  2:1264583/150‑1 (MQ=255)
                                                                     gcggcgGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGt                                                                             <  1:339126/150‑1 (MQ=255)
                                                                       ggcggcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGACTGGGAGTCGTTGTGTGATGGTTg                                                                           >  2:1576526/1‑150 (MQ=255)
                                                                           gcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGt                                                                       >  2:1729543/1‑150 (MQ=255)
                                                                              aaaCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAg                                                                    >  1:1856234/1‑150 (MQ=255)
                                                                               aaCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAgt                                                                   <  2:4579119/150‑1 (MQ=255)
                                                                               aaCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAgt                                                                   <  2:1440240/150‑1 (MQ=255)
                                                                                  ttGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGtt                                                                <  2:2927411/150‑1 (MQ=255)
                                                                                       tCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTg                                                           <  1:4524814/150‑1 (MQ=255)
                                                                                       tCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTg                                                           <  2:806485/150‑1 (MQ=255)
                                                                                       tCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTg                                                           <  2:4085560/150‑1 (MQ=255)
                                                                                          cTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCAt                                                        <  1:1757488/150‑1 (MQ=255)
                                                                                           tgtgCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAATTGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATa                                                       <  1:554916/150‑1 (MQ=255)
                                                                                           tgtgCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATa                                                       <  2:4700908/150‑1 (MQ=255)
                                                                                             tgCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATaaa                                                     >  2:2361434/1‑150 (MQ=255)
                                                                                                cAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTg                                                  <  2:3822523/150‑1 (MQ=255)
                                                                                                 aGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGAGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTga                                                 <  2:3668338/150‑1 (MQ=255)
                                                                                                  gACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTTCCTGCATAAACTgat                                                >  2:3987235/1‑150 (MQ=255)
                                                                                                     tGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatgga                                             <  1:3470274/150‑1 (MQ=255)
                                                                                                      ggTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGTTTCCTGCATAAAAtgattgat                                            >  1:86711/1‑150 (MQ=255)
                                                                                                      ggTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTTCGGTCAGTGTTGCCTGCATAAACtgattgat                                            >  1:3155541/1‑150 (MQ=255)
                                                                                                      ggTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggat                                            >  1:4616386/1‑150 (MQ=255)
                                                                                                      ggTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACAgatggat                                            >  1:1312040/1‑150 (MQ=255)
                                                                                                       gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGACTGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggatg                                           >  1:3836939/1‑150 (MQ=255)
                                                                                                        ttATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGa                                          >  2:3407246/1‑150 (MQ=255)
                                                                                                         tataAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGaa                                         <  2:2253511/150‑1 (MQ=255)
                                                                                                         tataAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGaa                                         <  2:2254475/150‑1 (MQ=255)
                                                                                                           taAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTTCGGTCAGTGTTTCCTGCATAAACTGATTGATGAAGa                                       >  1:4465817/1‑150 (MQ=255)
                                                                                                            aaGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGac                                      <  1:2395979/150‑1 (MQ=255)
                                                                                                                 gCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACcga                                 <  2:2041472/150‑1 (MQ=255)
                                                                                                                        aaCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATc                          <  2:778299/150‑1 (MQ=255)
                                                                                                                             aaTGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACtt                     >  1:4759516/1‑150 (MQ=255)
                                                                                                                             aaTGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGGGGGATGGTTGCGGTCCGTGTTGCCTGCAAAAACAGCAGGATGAAGACACCGACGAAAGCGACtt                     >  1:1342353/1‑150 (MQ=255)
                                                                                                                                ggCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCAc                  <  1:2351066/150‑1 (MQ=255)
                                                                                                                                   ggAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTaa               >  2:3138005/1‑150 (MQ=255)
                                                                                                                                    gAACACCTTATGATGAATTTTACTTTCTTTCAAATTAAAACCCTGGACGAAATTATCGATGCGGAATGGTAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGAATAAACTTATGGATGAAGACACCGACTAAATCTACTtaactaac              <  2:2107376/150‑1 (MQ=255)
                                                                                                                                     aaCACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGGGATGGTAGCGGTCAGTGTTGCCGGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACg             >  2:2823403/1‑150 (MQ=255)
                                                                                                                                      acacCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGt            <  2:1866895/150‑1 (MQ=255)
                                                                                                                                           tgatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCt       <  1:3018184/150‑1 (MQ=255)
                                                                                                                                            gatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTg      <  2:3337003/150‑1 (MQ=255)
                                                                                                                                            gatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGACTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTg      <  2:4762822/150‑1 (MQ=255)
                                                                                                                                             atgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGt     <  1:698111/150‑1 (MQ=255)
                                                                                                                                              tgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTTCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTc    >  2:3707692/1‑150 (MQ=255)
                                                                                                                                               gaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCGGTCg   >  2:2506839/1‑150 (MQ=255)
                                                                                                                                               gaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTAATTCACTAACGTCGCCTTTCg   >  2:4416512/1‑150 (MQ=255)
                                                                                                                                                aGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCCACGAAATCTACTTCACTAACGTCGCCTGTCGc  >  1:3345446/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGC  >  CP009273/1224145‑1224438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAG  >  CP009273/1224144‑1224441
                                                                                                                                                    |                                                                                                                                                     
GAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAA                                                                                                                                                      <  SRR19973078.1062827/150‑1 (MQ=60)
    TGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTA                                                                                                                                                  >  SRR19973078.2456455/1‑150 (MQ=60)
      ACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACC                                                                                                                                                >  SRR19973078.3892057/1‑150 (MQ=60)
      ACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACC                                                                                                                                                >  SRR19973078.3892165/1‑150 (MQ=60)
       CAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCT                                                                                                                                               <  SRR19973078.4807401/150‑1 (MQ=60)
        AGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTT                                                                                                                                              <  SRR19973078.3395538/150‑1 (MQ=60)
        AGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTT                                                                                                                                              <  SRR19973078.3869231/150‑1 (MQ=60)
          TCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTC                                                                                                                                            <  SRR19973078.798074/150‑1 (MQ=60)
            ACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTG                                                                                                                                          >  SRR19973078.2371923/1‑150 (MQ=60)
             CTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGG                                                                                                                                         <  SRR19973078.2534432/150‑1 (MQ=60)
             CTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGG                                                                                                                                         <  SRR19973078.127854/150‑1 (MQ=60)
               TTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCA                                                                                                                                       <  SRR19973078.3302332/150‑1 (MQ=60)
               TTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCA                                                                                                                                       <  SRR19973078.128778/150‑1 (MQ=60)
               TTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCA                                                                                                                                       >  SRR19973078.4748286/1‑150 (MQ=60)
                TCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACATTTCTGGCAA                                                                                                                                      >  SRR19973078.4789539/1‑150 (MQ=60)
                  GATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAG                                                                                                                                    >  SRR19973078.1000374/1‑150 (MQ=60)
                  GATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAG                                                                                                                                    >  SRR19973078.2277615/1‑150 (MQ=60)
                     GGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAA                                                                                                                                 >  SRR19973078.889584/1‑150 (MQ=60)
                      GGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAA                                                                                                                                <  SRR19973078.3192853/150‑1 (MQ=60)
                       GCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAA                                                                                                                               <  SRR19973078.2712929/150‑1 (MQ=60)
                        CATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAC                                                                                                                              >  SRR19973078.4174320/1‑150 (MQ=60)
                         ATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACC                                                                                                                             <  SRR19973078.2670824/150‑1 (MQ=60)
                         ATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACC                                                                                                                             <  SRR19973078.2670785/150‑1 (MQ=60)
                           TCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCT                                                                                                                           <  SRR19973078.844450/150‑1 (MQ=60)
                           TCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCT                                                                                                                           <  SRR19973078.844349/150‑1 (MQ=60)
                               TGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAC                                                                                                                       <  SRR19973078.540898/150‑1 (MQ=60)
                               TGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAC                                                                                                                       <  SRR19973078.540824/150‑1 (MQ=60)
                                GACAACTCGCTTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACG                                                                                                                      <  SRR19973078.1622786/150‑1 (MQ=60)
                                GACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACG                                                                                                                      <  SRR19973078.1622785/150‑1 (MQ=60)
                                 ACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGA                                                                                                                     >  SRR19973078.4798941/1‑150 (MQ=60)
                                 ACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGA                                                                                                                     >  SRR19973078.4798814/1‑150 (MQ=60)
                                  CAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAA                                                                                                                    >  SRR19973078.946846/1‑150 (MQ=60)
                                   AACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAA                                                                                                                   <  SRR19973078.1138922/150‑1 (MQ=60)
                                       CGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAG                                                                                                               <  SRR19973078.1875356/150‑1 (MQ=60)
                                       CGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAG                                                                                                               >  SRR19973078.3329892/1‑150 (MQ=60)
                                       CGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTAGACGAAATGAG                                                                                                               <  SRR19973078.1044978/150‑1 (MQ=60)
                                        GCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGC                                                                                                              <  SRR19973078.45267/150‑1 (MQ=60)
                                         CGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCG                                                                                                             >  SRR19973078.1917275/1‑150 (MQ=60)
                                         CGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCG                                                                                                             >  SRR19973078.1917233/1‑150 (MQ=60)
                                          GTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGA                                                                                                            >  SRR19973078.606451/1‑150 (MQ=60)
                                            TTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATG                                                                                                          <  SRR19973078.1153532/150‑1 (MQ=60)
                                            TTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATG                                                                                                          >  SRR19973078.3869427/1‑150 (MQ=60)
                                            TTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATG                                                                                                          >  SRR19973078.1448377/1‑150 (MQ=60)
                                            TTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATG                                                                                                          <  SRR19973078.352318/150‑1 (MQ=60)
                                            TTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATG                                                                                                          <  SRR19973078.3573853/150‑1 (MQ=60)
                                             TTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGC                                                                                                         <  SRR19973078.1167160/150‑1 (MQ=60)
                                                TAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGA                                                                                                      >  SRR19973078.420146/1‑150 (MQ=60)
                                                 AATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAA                                                                                                     <  SRR19973078.4325666/150‑1 (MQ=60)
                                                   TACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATG                                                                                                   <  SRR19973078.3691533/150‑1 (MQ=60)
                                                   TACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATG                                                                                                   >  SRR19973078.3792051/1‑150 (MQ=60)
                                                   TACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATG                                                                                                   >  SRR19973078.3792314/1‑150 (MQ=60)
                                                   TACTTTGTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATG                                                                                                   <  SRR19973078.3691503/150‑1 (MQ=60)
                                                    ACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGG                                                                                                  <  SRR19973078.594943/150‑1 (MQ=60)
                                                    ACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGG                                                                                                  <  SRR19973078.4284129/150‑1 (MQ=60)
                                                     CTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGG                                                                                                 <  SRR19973078.1005205/150‑1 (MQ=60)
                                                         TTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTC                                                                                             >  SRR19973078.34823/1‑150 (MQ=60)
                                                             CGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCACTGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGG                                                                                         >  SRR19973078.3627464/1‑150 (MQ=60)
                                                             CGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTT                                                                                         >  SRR19973078.4068250/1‑150 (MQ=60)
                                                             CGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGG                                                                                         >  SRR19973078.3627231/1‑150 (MQ=60)
                                                             CGCCTGAAAGCGGCGGCAAAACGTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTG                                                                                         <  SRR19973078.439661/150‑1 (MQ=60)
                                                               CCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTG                                                                                       >  SRR19973078.2591200/1‑150 (MQ=60)
                                                               CCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTG                                                                                       >  SRR19973078.2591365/1‑150 (MQ=60)
                                                               CCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTG                                                                                       >  SRR19973078.2591353/1‑150 (MQ=60)
                                                               CCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTG                                                                                       <  SRR19973078.688745/150‑1 (MQ=60)
                                                               CCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTG                                                                                       >  SRR19973078.2014538/1‑150 (MQ=60)
                                                                 TGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTG                                                                                     >  SRR19973078.3676811/1‑150 (MQ=60)
                                                                 TGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTG                                                                                     >  SRR19973078.3766345/1‑150 (MQ=60)
                                                                  GAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGA                                                                                    <  SRR19973078.1807640/150‑1 (MQ=60)
                                                                    AAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATG                                                                                  >  SRR19973078.1967344/1‑150 (MQ=60)
                                                                     AGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGG                                                                                 >  SRR19973078.2548622/1‑150 (MQ=60)
                                                                     AGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGG                                                                                 >  SRR19973078.2512266/1‑150 (MQ=60)
                                                                      GCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGT                                                                                <  SRR19973078.341308/150‑1 (MQ=60)
                                                                           GGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGG                                                                           <  SRR19973078.3161373/150‑1 (MQ=60)
                                                                              AAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCA                                                                        <  SRR19973078.1112579/150‑1 (MQ=60)
                                                                               AAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAG                                                                       >  SRR19973078.1867342/1‑150 (MQ=60)
                                                                                AACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGT                                                                      <  SRR19973078.756977/150‑1 (MQ=60)
                                                                                  CTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGT                                                                    <  SRR19973078.2962891/150‑1 (MQ=60)
                                                                                       ATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCT                                                               <  SRR19973078.1835661/150‑1 (MQ=60)
                                                                                        TCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTG                                                              <  SRR19973078.4550436/150‑1 (MQ=60)
                                                                                           CTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCAT                                                           <  SRR19973078.1768193/150‑1 (MQ=60)
                                                                                            TGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAATTGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATA                                                          <  SRR19973078.558599/150‑1 (MQ=60)
                                                                                            TGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATA                                                          <  SRR19973078.2712299/150‑1 (MQ=60)
                                                                                            TGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATA                                                          <  SRR19973078.559135/150‑1 (MQ=60)
                                                                                                  AGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGGCTGCATAAACTGA                                                    >  SRR19973078.4590243/1‑150 (MQ=60)
                                                                                                  AGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGA                                                    >  SRR19973078.2298736/1‑150 (MQ=60)
                                                                                                    ACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATG                                                  <  SRR19973078.4283824/150‑1 (MQ=60)
                                                                                                      TGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGA                                                <  SRR19973078.3490055/150‑1 (MQ=60)
                                                                                                       GGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGTTTCCTGCATAAAATGATTGAT                                               >  SRR19973078.87590/1‑150 (MQ=60)
                                                                                                       GGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTTCGGTCAGTGTTGCCTGCATAAACTGATTGAT                                               >  SRR19973078.3173170/1‑150 (MQ=60)
                                                                                                       GGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGAT                                               >  SRR19973078.4642535/1‑150 (MQ=60)
                                                                                                       GGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACAGATGGAT                                               >  SRR19973078.1320236/1‑150 (MQ=60)
                                                                                                       GGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCGGCAGAAACTGATGGAT                                               >  SRR19973078.1320341/1‑150 (MQ=60)
                                                                                                        GTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGACTGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATG                                              >  SRR19973078.3858921/1‑150 (MQ=60)
                                                                                                        GTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATG                                              <  SRR19973078.4255685/150‑1 (MQ=60)
                                                                                                            TAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTTCGGTCAGTGTTTCCTGCATAAACTGATTGATGAAGA                                          >  SRR19973078.4491078/1‑150 (MQ=60)
                                                                                                             AAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGAC                                         <  SRR19973078.335938/150‑1 (MQ=60)
                                                                                                             AAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGAC                                         <  SRR19973078.2409471/150‑1 (MQ=60)
                                                                                                                   CGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGGGTTTGTTTCGGTCAGTGTTGCCTGCATAAAAAGATGGATGTATACACCGAC                                   >  SRR19973078.5420/1‑150 (MQ=60)
                                                                                                                     TTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATTGATGAACACACCGACGA                                 >  SRR19973078.4609515/1‑150 (MQ=60)
                                                                                                                        TAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAAT                              <  SRR19973078.3142020/150‑1 (MQ=60)
                                                                                                                           CGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTA                           >  SRR19973078.3974903/1‑150 (MQ=60)
                                                                                                                              AATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTATGTTGAGGTCAGTGTTGCCTGCcaaaacatattgaataagacaccgaacaaaactacat                        >  SRR19973078.3734690/1‑113 (MQ=60)
                                                                                                                              AATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTT                        >  SRR19973078.4786229/1‑150 (MQ=60)
                                                                                                                              AATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTT                        <  SRR19973078.3864495/150‑1 (MQ=60)
                                                                                                                              AATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGGGGGATGGTTGCGGTCCGTGTTGCCTGCAAAAACAGCAGGATGAAGACACCGACGAAAGCGACTT                        >  SRR19973078.1350758/1‑150 (MQ=60)
                                                                                                                               ATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTC                       <  SRR19973078.2062438/150‑1 (MQ=60)
                                                                                                                                 GGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCAC                     <  SRR19973078.2364408/150‑1 (MQ=60)
                                                                                                                                    GGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTTCCTGCATAAACTGATGGATGAAGACACCGACGAAATATACATCACTAA                  >  SRR19973078.2291298/1‑150 (MQ=60)
                                                                                                                                    GGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAA                  >  SRR19973078.4708148/1‑150 (MQ=60)
                                                                                                                                           CTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCC           >  SRR19973078.3520324/1‑150 (MQ=60)
                                                                                                                                            TGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCT          <  SRR19973078.3035362/150‑1 (MQ=60)
                                                                                                                                            TGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCT          <  SRR19973078.1715949/150‑1 (MQ=60)
                                                                                                                                            TGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGGGTGTTGGTTGCGGTCAGTGTTGCCTGCATAAAAAGATGGATGAAGACACCcaccaaaacaacatcacaaaccgcgcca          >  SRR19973078.2041877/1‑122 (MQ=60)
                                                                                                                                            TGATGAGCAATGGACCTTTCTGGCACAGTAAAACCCTGGACGAAATGAGCGATGCGGTATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACGTCACTAACGTCGCCG          <  SRR19973078.630651/150‑1 (MQ=60)
                                                                                                                                             GATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTG         <  SRR19973078.1335248/150‑1 (MQ=60)
                                                                                                                                              ATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGT        <  SRR19973078.702437/150‑1 (MQ=60)
                                                                                                                                              ATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGT        <  SRR19973078.4020203/150‑1 (MQ=60)
                                                                                                                                                GAGCAATGGACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCG      <  SRR19973078.3474889/150‑1 (MQ=60)
                                                                                                                                                 AGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCCGTGTTGCCTGCCTAAACTGATGGATGAATAAACCGACGAAATCTACTTAACTAACCTCGCCTGTCGC     >  SRR19973078.3364867/1‑150 (MQ=60)
                                                                                                                                                 AGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCCACGAAATCTACTTCACTAACGTCGCCTGTCGC     >  SRR19973078.3364573/1‑150 (MQ=60)
                                                                                                                                                    AATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAG  <  SRR19973078.646981/150‑1 (MQ=60)
                                                                                                                                                    AATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACATCACTAACGTCGCCAGTCGCCAG  >  SRR19973078.185567/1‑150 (MQ=60)
                                                                                                                                                    AATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACATCACAAACGTCGCCAGTCGCCAG  >  SRR19973078.2588952/1‑150 (MQ=60)
                                                                                                                                                    AATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCCACGAAATCTACTTCACTAACGTCGCCAGTCGCCAG  >  SRR19973078.4461099/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAG  >  CP009273/1224144‑1224441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑