Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,451,422 A→T 100% T246S (ACG→TCG)  paaE → ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,451,4220AT96.5% 740.0 / 12.0 230T246S (ACG→TCG) paaEring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
Reads supporting (aligned to +/- strand):  ref base A (1/7);  new base T (129/93);  total (130/100)
Fisher's exact test for biased strand distribution p-value = 2.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

CAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGG  >  CP009273/1451278‑1451566
                                                                                                                                                |                                                                                                                                                
cAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGc                                                                                                                                               <  1:1173711/148‑1 (MQ=255)
 aGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGcc                                                                                                                                              <  1:3467912/148‑1 (MQ=255)
  gTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCt                                                                                                                                             >  2:1637314/1‑148 (MQ=255)
   tCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTg                                                                                                                                            <  2:942966/148‑1 (MQ=255)
   tCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTg                                                                                                                                            >  1:1255304/1‑148 (MQ=255)
   tCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTg                                                                                                                                            <  2:2622996/148‑1 (MQ=255)
        tGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCAcg                                                                                                                                       <  1:1019586/148‑1 (MQ=255)
            gCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCAcgcgcg                                                                                                                                   >  2:2151920/1‑148 (MQ=255)
               tCGCTCATTAATTTTCGTCTTTATGATGAGGTATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCa                                                                                                                                >  1:322074/1‑148 (MQ=255)
                cGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCaa                                                                                                                               <  1:2254927/148‑1 (MQ=255)
                cGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCaa                                                                                                                               >  2:1625696/1‑148 (MQ=255)
                   tCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACg                                                                                                                            >  2:1970672/1‑148 (MQ=255)
                    cATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGt                                                                                                                           <  1:196788/148‑1 (MQ=255)
                    cATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGt                                                                                                                           >  2:1385062/1‑148 (MQ=255)
                    cATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGt                                                                                                                           >  1:303441/1‑148 (MQ=255)
                      ttAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAg                                                                                                                         >  2:3289240/1‑148 (MQ=255)
                        aaTTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCg                                                                                                                       >  2:3443444/1‑148 (MQ=255)
                         aTTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGt                                                                                                                      >  1:2290484/1‑148 (MQ=255)
                           tttCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTa                                                                                                                    >  2:3210912/1‑148 (MQ=255)
                              cGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACg                                                                                                                 >  2:2339033/1‑148 (MQ=255)
                              cGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACg                                                                                                                 <  1:1644027/148‑1 (MQ=255)
                              cGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTAGCCTGGCACGCGCGTCAAACGTAGCGTTAACg                                                                                                                 >  1:2937092/1‑148 (MQ=255)
                                tCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTg                                                                                                               >  2:796288/1‑148 (MQ=255)
                                tCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTg                                                                                                               <  2:968401/148‑1 (MQ=255)
                                 cTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGc                                                                                                              >  1:351706/1‑148 (MQ=255)
                                 cTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGc                                                                                                              >  1:2148364/1‑148 (MQ=255)
                                   ttATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCaa                                                                                                            <  2:1075026/148‑1 (MQ=255)
                                   ttATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCaa                                                                                                            >  1:279279/1‑148 (MQ=255)
                                   ttATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCaa                                                                                                            >  1:3081888/1‑148 (MQ=255)
                                     atgatgAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAg                                                                                                          <  1:412753/148‑1 (MQ=255)
                                     atgatgAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAg                                                                                                          <  2:811986/148‑1 (MQ=255)
                                        atgaGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGa                                                                                                       >  1:523833/1‑148 (MQ=255)
                                           aGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACgg                                                                                                    <  1:2161921/148‑1 (MQ=255)
                                            ggCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGa                                                                                                   >  1:296423/1‑148 (MQ=255)
                                             gCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGAc                                                                                                  >  2:2180761/1‑148 (MQ=255)
                                              cATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACa                                                                                                 >  2:3139772/1‑148 (MQ=255)
                                              cATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACa                                                                                                 >  2:194157/1‑148 (MQ=255)
                                                 ttatttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACaaaa                                                                                              >  1:1232901/1‑148 (MQ=255)
                                                 ttatttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACaaaa                                                                                              <  2:3066375/148‑1 (MQ=255)
                                                  tatttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACaaaaa                                                                                             >  2:1917536/1‑148 (MQ=255)
                                                   atttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAg                                                                                            <  2:1715713/148‑1 (MQ=255)
                                                    tttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGt                                                                                           >  2:1597355/1‑148 (MQ=255)
                                                      tgtgGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGa                                                                                         <  1:3066506/148‑1 (MQ=255)
                                                              ggcggcGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGt                                                                                 <  1:1833885/148‑1 (MQ=255)
                                                              ggcggcGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGt                                                                                 <  1:2180761/148‑1 (MQ=255)
                                                               gcggcgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCGAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTc                                                                                >  2:861521/1‑148 (MQ=255)
                                                                cggcgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCa                                                                               <  1:3289240/148‑1 (MQ=255)
                                                                cggcgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCa                                                                               <  1:1636230/148‑1 (MQ=255)
                                                                   cgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGa                                                                            <  2:372553/148‑1 (MQ=255)
                                                                   cgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGa                                                                            <  1:2692369/148‑1 (MQ=255)
                                                                    gatgatGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGAt                                                                           >  2:2259175/1‑148 (MQ=255)
                                                                     atgatgGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATg                                                                          >  2:246772/1‑148 (MQ=255)
                                                                        atggatgACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGc                                                                       >  1:2554170/1‑148 (MQ=255)
                                                                        atggatgACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGc                                                                       >  1:878257/1‑148 (MQ=255)
                                                                           gatgACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCggg                                                                    >  2:1281218/1‑148 (MQ=255)
                                                                           gatgACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCggg                                                                    >  1:3172352/1‑148 (MQ=255)
                                                                               aCGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCg                                                                >  1:1347473/1‑148 (MQ=255)
                                                                                cgcgGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAACCCAGTCATCTGGAGAGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCgg                                                               <  1:445225/148‑1 (MQ=255)
                                                                                cgcgGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCgg                                                               <  1:1704136/148‑1 (MQ=255)
                                                                                     aaaCCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAAt                                                          >  2:3027116/1‑148 (MQ=255)
                                                                                       aCCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCg                                                        >  1:2057875/1‑148 (MQ=255)
                                                                                       aCCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCg                                                        >  2:554824/1‑148 (MQ=255)
                                                                                        ccGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGt                                                       <  2:1179703/148‑1 (MQ=255)
                                                                                            cTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCg                                                        <  2:2722440/143‑1 (MQ=255)
                                                                                             ttAAAAGCACTGGGAATGCCATATAAAACCATTCATCTGGATCGTTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTa                                                  <  1:2293868/148‑1 (MQ=255)
                                                                                             ttAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTa                                                  <  1:1520992/148‑1 (MQ=255)
                                                                                             ttAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTa                                                  <  1:3225363/148‑1 (MQ=255)
                                                                                                  aGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGcc                                             >  2:2519757/1‑148 (MQ=255)
                                                                                                    cACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCcga                                           >  1:1467562/1‑148 (MQ=255)
                                                                                                    cACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCcga                                           >  1:947764/1‑148 (MQ=255)
                                                                                                      cTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCcgacg                                         >  2:2062038/1‑148 (MQ=255)
                                                                                                        gggAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGAt                                       >  2:1154106/1‑148 (MQ=255)
                                                                                                         ggAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATg                                      <  1:2634394/148‑1 (MQ=255)
                                                                                                            aTGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGaaa                                   <  1:782219/148‑1 (MQ=255)
                                                                                                            aTGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGaaa                                   >  2:2192844/1‑148 (MQ=255)
                                                                                                            aTGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGaaa                                   <  2:279279/148‑1 (MQ=255)
                                                                                                             tGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAg                                  >  1:3242683/1‑148 (MQ=255)
                                                                                                              gCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGc                                 <  1:1625594/148‑1 (MQ=255)
                                                                                                               ccAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCa                                >  2:1014523/1‑148 (MQ=255)
                                                                                                               ccAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCa                                <  1:2082394/148‑1 (MQ=255)
                                                                                                               ccAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCa                                <  1:2339033/148‑1 (MQ=255)
                                                                                                                 aGATAAAACCATTCATCTGGAGCGGTTTAATTCGTCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCAtt                              >  1:1972559/1‑148 (MQ=255)
                                                                                                                  gATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACACAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtc                             >  2:143374/1‑148 (MQ=255)
                                                                                                                  gATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtc                             >  2:1168401/1‑148 (MQ=255)
                                                                                                                  gATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtc                             >  1:1096744/1‑148 (MQ=255)
                                                                                                                  gATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtc                             <  1:681401/148‑1 (MQ=255)
                                                                                                                   aTAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtct                            <  1:1001036/148‑1 (MQ=255)
                                                                                                                     aaaaCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCg                          >  2:700528/1‑148 (MQ=255)
                                                                                                                      aaaCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGa                         <  1:3139772/148‑1 (MQ=255)
                                                                                                                       aaCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGAt                        >  2:826232/1‑148 (MQ=255)
                                                                                                                        aCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATg                       <  1:1243833/148‑1 (MQ=255)
                                                                                                                        aCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATg                       <  1:3210912/148‑1 (MQ=255)
                                                                                                                             tCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGTGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCa                  <  1:1907385/148‑1 (MQ=255)
                                                                                                                               aTCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCAtt                >  1:2627715/1‑148 (MQ=255)
                                                                                                                                tCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTg               >  1:3457267/1‑148 (MQ=255)
                                                                                                                                      gCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAg         <  2:423534/148‑1 (MQ=255)
                                                                                                                                      gCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAg         <  2:22802/148‑1 (MQ=255)
                                                                                                                                       cGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgg        >  2:2956495/1‑148 (MQ=255)
                                                                                                                                       cGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgg        >  2:1046965/1‑148 (MQ=255)
                                                                                                                                            tAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCg   >  2:3318323/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGG  >  CP009273/1451278‑1451566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATC  >  CP009273/1451273‑1451569
                                                                                                                                                     |                                                                                                                                                   
AATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATT                                                                                                                                                     >  SRR19973066.2112099/1‑150 (MQ=60)
 ATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTC                                                                                                                                                    <  SRR19973066.2230141/150‑1 (MQ=60)
 ATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACAGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTC                                                                                                                                                    >  SRR19973066.3163978/1‑150 (MQ=60)
  TTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCG                                                                                                                                                   <  SRR19973066.2273444/150‑1 (MQ=60)
     CAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCT                                                                                                                                                <  SRR19973066.1181989/150‑1 (MQ=60)
      AGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTG                                                                                                                                               >  SRR19973066.1265027/1‑150 (MQ=60)
      AGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTG                                                                                                                                               >  SRR19973066.1264342/1‑150 (MQ=60)
      AGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTG                                                                                                                                               >  SRR19973066.1264323/1‑150 (MQ=60)
      AGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTG                                                                                                                                               >  SRR19973066.92272/1‑150 (MQ=60)
      AGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTG                                                                                                                                               <  SRR19973066.3582617/150‑1 (MQ=60)
      AGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTG                                                                                                                                               <  SRR19973066.3491087/150‑1 (MQ=60)
      AGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTG                                                                                                                                               <  SRR19973066.3490447/150‑1 (MQ=60)
             TGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCG                                                                                                                                        <  SRR19973066.1027088/150‑1 (MQ=60)
             TGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCG                                                                                                                                        <  SRR19973066.1027089/150‑1 (MQ=60)
             TGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCG                                                                                                                                        >  SRR19973066.1982008/1‑150 (MQ=60)
                 GCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTC                                                                                                                                    <  SRR19973066.1792665/150‑1 (MQ=60)
                 GCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTC                                                                                                                                    <  SRR19973066.19030/150‑1 (MQ=60)
                  CCTCGCTCATTAATTTTCGTCTTTATGATGAGGTATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCA                                                                                                                                   >  SRR19973066.324149/1‑150 (MQ=60)
                  CCTCGCTCATTAATTTTCGTCTTTATGATGAGGTATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCA                                                                                                                                   >  SRR19973066.324169/1‑150 (MQ=60)
                  CCTCGCTCATTAATTTTCGTCTTTATGATGAGGTATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCA                                                                                                                                   >  SRR19973066.324499/1‑150 (MQ=60)
                     CGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAAC                                                                                                                                <  SRR19973066.2270469/150‑1 (MQ=60)
                     CGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAAC                                                                                                                                <  SRR19973066.2270341/150‑1 (MQ=60)
                     CGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAAC                                                                                                                                >  SRR19973066.503322/1‑150 (MQ=60)
                     CGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAAC                                                                                                                                >  SRR19973066.120547/1‑150 (MQ=60)
                       CTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGT                                                                                                                              >  SRR19973066.305599/1‑150 (MQ=60)
                       CTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGT                                                                                                                              >  SRR19973066.305509/1‑150 (MQ=60)
                         CATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAG                                                                                                                            <  SRR19973066.198474/150‑1 (MQ=60)
                         CATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAG                                                                                                                            <  SRR19973066.2096286/150‑1 (MQ=60)
                         CATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAG                                                                                                                            <  SRR19973066.1013665/150‑1 (MQ=60)
                            TAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGT                                                                                                                         >  SRR19973066.2306218/1‑150 (MQ=60)
                            TAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGT                                                                                                                         >  SRR19973066.2306192/1‑150 (MQ=60)
                            TAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGT                                                                                                                         >  SRR19973066.2305970/1‑150 (MQ=60)
                                 TTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTAGCCTGGCACGCGCGTCAAACGTAGCGTTAACG                                                                                                                    >  SRR19973066.2956627/1‑150 (MQ=60)
                                   CGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTG                                                                                                                  <  SRR19973066.1655993/150‑1 (MQ=60)
                                   CGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTG                                                                                                                  <  SRR19973066.1574461/150‑1 (MQ=60)
                                    GTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGC                                                                                                                 >  SRR19973066.2163277/1‑150 (MQ=60)
                                    GTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGC                                                                                                                 >  SRR19973066.354446/1‑150 (MQ=60)
                                      CTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAA                                                                                                               >  SRR19973066.281312/1‑150 (MQ=60)
                                      CTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAA                                                                                                               >  SRR19973066.3102474/1‑150 (MQ=60)
                                        TTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAG                                                                                                             <  SRR19973066.608559/150‑1 (MQ=60)
                                          ATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTG                                                                                                           <  SRR19973066.415983/150‑1 (MQ=60)
                                           TGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGA                                                                                                          >  SRR19973066.2508010/1‑150 (MQ=60)
                                           TGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGA                                                                                                          >  SRR19973066.527675/1‑150 (MQ=60)
                                               GAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGA                                                                                                      >  SRR19973066.297582/1‑150 (MQ=60)
                                               GAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGA                                                                                                      >  SRR19973066.2621426/1‑150 (MQ=60)
                                               GAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGA                                                                                                      >  SRR19973066.298527/1‑150 (MQ=60)
                                                AGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGAC                                                                                                     <  SRR19973066.2176941/150‑1 (MQ=60)
                                                AGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGAC                                                                                                     <  SRR19973066.2176952/150‑1 (MQ=60)
                                                  GCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAA                                                                                                   >  SRR19973066.438465/1‑150 (MQ=60)
                                                    ATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAA                                                                                                 >  SRR19973066.1241659/1‑150 (MQ=60)
                                                      TTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAG                                                                                               <  SRR19973066.2031144/150‑1 (MQ=60)
                                                         TTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGA                                                                                            >  SRR19973066.2375419/1‑150 (MQ=60)
                                                         TTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGA                                                                                            >  SRR19973066.1527405/1‑150 (MQ=60)
                                                           TGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACT                                                                                          <  SRR19973066.3087012/150‑1 (MQ=60)
                                                           TGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACT                                                                                          <  SRR19973066.3119877/150‑1 (MQ=60)
                                                                   ggctgcgatgttgtttttcgcgtAAACCTCCTTAAAAGAACTGGGATTGCCAGATAAAACCATTAATCTGTAGCGTTTTATTTCGCCTGTCAGGCGCTTCAAACGTAGCTTTACCGTGCAAAGTGCCGGACAAAAAGTGACTGTACGTCA                                                                                  <  SRR19973066.1846990/127‑1 (MQ=55)
                                                                   GGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCA                                                                                  <  SRR19973066.1846982/150‑1 (MQ=60)
                                                                   GGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCA                                                                                  <  SRR19973066.2195897/150‑1 (MQ=60)
                                                                     CGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGG                                                                                <  SRR19973066.1649219/150‑1 (MQ=60)
                                                                     CGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGG                                                                                <  SRR19973066.1648125/150‑1 (MQ=60)
                                                                     CGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGG                                                                                <  SRR19973066.3310927/150‑1 (MQ=60)
                                                                      GGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGA                                                                               >  SRR19973066.2271040/1‑150 (MQ=60)
                                                                        CGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATG                                                                             <  SRR19973066.195825/150‑1 (MQ=60)
                                                                        CGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATG                                                                             <  SRR19973066.2707599/150‑1 (MQ=60)
                                                                        CGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATG                                                                             <  SRR19973066.2710349/150‑1 (MQ=60)
                                                                          ATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGG                                                                           <  SRR19973066.560250/150‑1 (MQ=60)
                                                                           TGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGC                                                                          >  SRR19973066.2571516/1‑150 (MQ=60)
                                                                           TGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGC                                                                          >  SRR19973066.884516/1‑150 (MQ=60)
                                                                              TGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGG                                                                       >  SRR19973066.1981925/1‑150 (MQ=60)
                                                                              TGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGG                                                                       >  SRR19973066.3193475/1‑150 (MQ=60)
                                                                              TGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGG                                                                       >  SRR19973066.3193430/1‑150 (MQ=60)
                                                                               GGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGA                                                                      >  SRR19973066.1157558/1‑150 (MQ=60)
                                                                                GATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGAT                                                                     >  SRR19973066.2348915/1‑150 (MQ=60)
                                                                                GATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGAT                                                                     >  SRR19973066.986965/1‑150 (MQ=60)
                                                                                 ATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATC                                                                    <  SRR19973066.3291289/150‑1 (MQ=60)
                                                                                  TGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCG                                                                   >  SRR19973066.3140232/1‑150 (MQ=60)
                                                                                  TGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCG                                                                   >  SRR19973066.1357245/1‑150 (MQ=60)
                                                                                     CGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAACCCAGTCATCTGGAGAGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGA                                                                <  SRR19973066.448655/150‑1 (MQ=60)
                                                                                     CGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGA                                                                <  SRR19973066.1716510/150‑1 (MQ=60)
                                                                                          AAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCG                                                           >  SRR19973066.2072153/1‑150 (MQ=60)
                                                                                          AAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCG                                                           <  SRR19973066.21158/150‑1 (MQ=60)
                                                                                              CGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCT                                                       <  SRR19973066.3410791/150‑1 (MQ=60)
                                                                                              CGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCT                                                       >  SRR19973066.3041925/1‑150 (MQ=60)
                                                                                               GCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGaga                                                      >  SRR19973066.2740754/1‑147 (MQ=60)
                                                                                                CCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTA                                                     >  SRR19973066.820946/1‑150 (MQ=60)
                                                                                                  TTAAATGCTCTGGGTATGCCAGATAAAACCATTAATCCGGAGCGGTTTATTTCGCCTGGCACGCGCGTCATAGGTAGCGTTTAAGTGCAAATTGCCGGACAAAAAGTGACTGTTCGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAAT                                                   <  SRR19973066.1705081/150‑1 (MQ=60)
                                                                                                  TTAAAAGCACTGGGAATGCCATATAAAACCATTCATCTGGATCGTTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAAT                                                   <  SRR19973066.2309596/150‑1 (MQ=60)
                                                                                                  TTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAAT                                                   <  SRR19973066.3246687/150‑1 (MQ=60)
                                                                                                  TTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAAT                                                   <  SRR19973066.1531719/150‑1 (MQ=60)
                                                                                                   TAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTCGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATG                                                  >  SRR19973066.1329413/1‑150 (MQ=60)
                                                                                                       AGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGACATCGTGCTTAATGCCGA                                              >  SRR19973066.954682/1‑150 (MQ=60)
                                                                                                       AGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGA                                              >  SRR19973066.1477919/1‑150 (MQ=60)
                                                                                                        GCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGAC                                             <  SRR19973066.406646/150‑1 (MQ=60)
                                                                                                          ACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACTA                                           >  SRR19973066.3259477/1‑150 (MQ=60)
                                                                                                          ACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGA                                           >  SRR19973066.1476400/1‑150 (MQ=60)
                                                                                                            TGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTTACGTCAAAAAAGTGCCGGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATG                                         <  SRR19973066.3520811/150‑1 (MQ=60)
                                                                                                              GGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAA                                       <  SRR19973066.2652133/150‑1 (MQ=60)
                                                                                                              GGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAA                                       >  SRR19973066.734541/1‑150 (MQ=60)
                                                                                                                AATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAG                                     >  SRR19973066.3264073/1‑150 (MQ=60)
                                                                                                                 ATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGC                                    <  SRR19973066.787993/150‑1 (MQ=60)
                                                                                                                  TGCCAGATACAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCA                                   <  SRR19973066.3395416/150‑1 (MQ=60)
                                                                                                                  TGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCA                                   >  SRR19973066.1604521/1‑150 (MQ=60)
                                                                                                                  TGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCA                                   >  SRR19973066.310350/1‑150 (MQ=60)
                                                                                                                   GCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCAT                                  <  SRR19973066.1637379/150‑1 (MQ=60)
                                                                                                                   GCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCAT                                  <  SRR19973066.1637482/150‑1 (MQ=60)
                                                                                                                    CCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGTCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATT                                 >  SRR19973066.1986497/1‑150 (MQ=60)
                                                                                                                    CCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATT                                 <  SRR19973066.2354964/150‑1 (MQ=60)
                                                                                                                    CCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATT                                 <  SRR19973066.2096765/150‑1 (MQ=60)
                                                                                                                     CAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTC                                >  SRR19973066.1104717/1‑150 (MQ=60)
                                                                                                                       GATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTC                              <  SRR19973066.686401/150‑1 (MQ=60)
                                                                                                                        ATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCG                             <  SRR19973066.2166861/150‑1 (MQ=60)
                                                                                                                        ATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCG                             <  SRR19973066.1008415/150‑1 (MQ=60)
                                                                                                                           AAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATG                          >  SRR19973066.2121752/1‑150 (MQ=60)
                                                                                                                           AAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATG                          <  SRR19973066.3160721/150‑1 (MQ=60)
                                                                                                                             ACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGTCGACGATGAAAGCATTCTCGATGCG                        >  SRR19973066.2674930/1‑150 (MQ=60)
                                                                                                                             ACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCG                        <  SRR19973066.3232193/150‑1 (MQ=60)
                                                                                                                             ACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCG                        <  SRR19973066.3231980/150‑1 (MQ=60)
                                                                                                                             ACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCG                        <  SRR19973066.1252742/150‑1 (MQ=60)
                                                                                                                             ACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCG                        <  SRR19973066.1251823/150‑1 (MQ=60)
                                                                                                                              CCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGG                       >  SRR19973066.1761411/1‑150 (MQ=60)
                                                                                                                              CCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGG                       >  SRR19973066.2824207/1‑150 (MQ=60)
                                                                                                                                  TCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGTGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATT                   <  SRR19973066.1920975/150‑1 (MQ=60)
                                                                                                                                  TCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATT                   >  SRR19973066.2645862/1‑150 (MQ=60)
                                                                                                                                  TCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATT                   >  SRR19973066.2645423/1‑150 (MQ=60)
                                                                                                                                   CATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTG                  >  SRR19973066.3480382/1‑150 (MQ=60)
                                                                                                                                     TCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCG                <  SRR19973066.1199175/150‑1 (MQ=60)
                                                                                                                                             GGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGATAACGAGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGG        >  SRR19973066.3246342/1‑150 (MQ=60)
                                                                                                                                                  AATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGAT   <  SRR19973066.3059444/150‑1 (MQ=60)
                                                                                                                                                   ATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATC  >  SRR19973066.2725941/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATC  >  CP009273/1451273‑1451569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑