Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,451,422 A→T 100% T246S (ACG→TCG)  paaE → ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,451,4220AT99.1% 767.1 / ‑2.7 232T246S (ACG→TCG) paaEring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (133/97);  total (135/97)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

CAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCG  >  CP009273/1451278‑1451565
                                                                                                                                                |                                                                                                                                               
   tCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTg                                                                                                                                           >  1:1651825/1‑148 (MQ=255)
     aCTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGACACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGc                                                                                                                                         >  2:119819/1‑148 (MQ=255)
               tCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCa                                                                                                                               >  1:2140849/1‑148 (MQ=255)
                cGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCaa                                                                                                                              >  1:1200275/1‑148 (MQ=255)
                 gCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCaaa                                                                                                                             <  1:3305257/148‑1 (MQ=255)
                  cTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAAc                                                                                                                            >  2:3287629/1‑148 (MQ=255)
                  cTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAAc                                                                                                                            <  1:998133/148‑1 (MQ=255)
                   tCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACg                                                                                                                           >  2:2370318/1‑148 (MQ=255)
                     aTTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTa                                                                                                                         >  2:179748/1‑148 (MQ=255)
                     aTTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTa                                                                                                                         >  1:414951/1‑148 (MQ=255)
                       tAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGc                                                                                                                       >  1:1196789/1‑148 (MQ=255)
                       tAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGc                                                                                                                       >  1:2947321/1‑148 (MQ=255)
                         aTTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGt                                                                                                                     <  1:1090655/148‑1 (MQ=255)
                              cGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACg                                                                                                                >  2:2959163/1‑148 (MQ=255)
                              cGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACg                                                                                                                >  2:1209291/1‑148 (MQ=255)
                                 cTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGACTGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTCGCGTTAACGTGc                                                                                                             >  2:1838134/1‑148 (MQ=255)
                                 cTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGc                                                                                                             >  1:827835/1‑148 (MQ=255)
                                   ttATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCaa                                                                                                           >  2:628495/1‑148 (MQ=255)
                                    tATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCaaa                                                                                                          >  2:139518/1‑148 (MQ=255)
                                      tgatgaGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGt                                                                                                        <  1:1013692/148‑1 (MQ=255)
                                        atgaGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGa                                                                                                      <  2:3128426/148‑1 (MQ=255)
                                        atgaGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGa                                                                                                      >  1:1277932/1‑148 (MQ=255)
                                          gaGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACg                                                                                                    >  2:1549585/1‑148 (MQ=255)
                                          gaGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACg                                                                                                    <  1:592612/148‑1 (MQ=255)
                                            ggCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGa                                                                                                  <  2:2938228/148‑1 (MQ=255)
                                             gCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGAc                                                                                                 >  1:3166100/1‑148 (MQ=255)
                                                 ttatttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACaaaa                                                                                             >  1:3043732/1‑148 (MQ=255)
                                                   atttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAg                                                                                           <  2:265307/148‑1 (MQ=255)
                                                     ttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTg                                                                                         >  1:2662971/1‑148 (MQ=255)
                                                     ttGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTg                                                                                         <  1:2021175/148‑1 (MQ=255)
                                                      tgtgGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGa                                                                                        <  1:2682507/148‑1 (MQ=255)
                                                      tgtgGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGa                                                                                        <  2:3166100/148‑1 (MQ=255)
                                                      tgtgGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGa                                                                                        <  2:3420033/148‑1 (MQ=255)
                                                      tgtgGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGa                                                                                        <  1:739588/148‑1 (MQ=255)
                                                      tgtgGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGa                                                                                        <  1:2747822/148‑1 (MQ=255)
                                                       gtgGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGAc                                                                                       >  2:813393/1‑148 (MQ=255)
                                                          gTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGTACAAAAAGTGACTGt                                                                                    <  1:670476/148‑1 (MQ=255)
                                                            ccGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTAc                                                                                  >  2:3060866/1‑148 (MQ=255)
                                                            ccGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTAc                                                                                  >  2:1313623/1‑148 (MQ=255)
                                                               gcggcgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTc                                                                               >  2:2687368/1‑148 (MQ=255)
                                                                cggcgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCa                                                                              >  1:1962653/1‑148 (MQ=255)
                                                                  gcgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAgg                                                                            <  1:2407343/148‑1 (MQ=255)
                                                                  gcgATGATGGATGACGCAGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAgg                                                                            <  2:2909043/148‑1 (MQ=255)
                                                                   cgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGa                                                                           <  1:1598001/148‑1 (MQ=255)
                                                                   cgATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGa                                                                           <  1:2936191/148‑1 (MQ=255)
                                                                      tgatgGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATgg                                                                        <  1:946319/148‑1 (MQ=255)
                                                                      tgatgGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATgg                                                                        >  2:1553313/1‑148 (MQ=255)
                                                                         tggatgACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCg                                                                     <  1:3049757/148‑1 (MQ=255)
                                                                          ggatgACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCgg                                                                    <  1:879738/148‑1 (MQ=255)
                                                                                 gcgGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCggg                                                             <  1:1161482/148‑1 (MQ=255)
                                                                                   ggAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGaa                                                           <  1:2296631/148‑1 (MQ=255)
                                                                                          gCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGc                                                    <  1:2100414/148‑1 (MQ=255)
                                                                                          gCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGc                                                    <  1:2100382/148‑1 (MQ=255)
                                                                                           ccTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCt                                                   >  2:464900/1‑148 (MQ=255)
                                                                                           ccTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCt                                                   <  1:2081695/148‑1 (MQ=255)
                                                                                             ttAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTa                                                 >  2:3269468/1‑148 (MQ=255)
                                                                                              tAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTaa                                                <  2:1020243/148‑1 (MQ=255)
                                                                                                   gCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCcg                                           >  1:3317344/1‑148 (MQ=255)
                                                                                                      cTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCcgacg                                        <  1:2537051/148‑1 (MQ=255)
                                                                                                        gggAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGAt                                      >  2:3142833/1‑148 (MQ=255)
                                                                                                           aaTGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGaa                                   <  1:2505431/148‑1 (MQ=255)
                                                                                                           aaTGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGaa                                   >  2:336230/1‑148 (MQ=255)
                                                                                                            aTGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGaaa                                  >  1:3492508/1‑148 (MQ=255)
                                                                                                             tGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAg                                 >  1:294347/1‑148 (MQ=255)
                                                                                                              gCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGc                                <  2:414951/148‑1 (MQ=255)
                                                                                                               ccAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCa                               >  1:1354126/1‑148 (MQ=255)
                                                                                                               ccAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCa                               <  2:242161/148‑1 (MQ=255)
                                                                                                                 aGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCAtt                             <  1:2206951/148‑1 (MQ=255)
                                                                                                                   aTAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtct                           >  1:2638565/1‑148 (MQ=255)
                                                                                                                   aTAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATtct                           >  2:2207959/1‑148 (MQ=255)
                                                                                                                     aaaaCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCg                         >  2:2203241/1‑148 (MQ=255)
                                                                                                                      aaaCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGa                        <  2:2040109/148‑1 (MQ=255)
                                                                                                                      aaaCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGa                        <  1:422681/148‑1 (MQ=255)
                                                                                                                       aaCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGAt                       >  2:2091293/1‑148 (MQ=255)
                                                                                                                        aCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATg                      >  1:2313187/1‑148 (MQ=255)
                                                                                                                         ccATTCATCTGGAGCGGTTTAATTAGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGAAGGACAAAAAGTGAATGTACGACAGGATGGGCGGGATCGGGAAATAGTGCTTAATGACGACGATGAAAGCATTCACGATga                     >  1:3026933/1‑147 (MQ=255)
                                                                                                                          cATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCg                    >  1:1122723/1‑148 (MQ=255)
                                                                                                                           aTTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCgg                   >  2:3163695/1‑148 (MQ=255)
                                                                                                                              cATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCAt                >  2:438111/1‑148 (MQ=255)
                                                                                                                               aTCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCAtt               >  1:2367631/1‑148 (MQ=255)
                                                                                                                                tCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTg              >  2:913596/1‑148 (MQ=255)
                                                                                                                                tCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTg              >  2:2009290/1‑148 (MQ=255)
                                                                                                                                tCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTg              <  1:1465919/148‑1 (MQ=255)
                                                                                                                                 cTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTgc             >  2:2597950/1‑148 (MQ=255)
                                                                                                                                  tGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTgcg            <  2:626645/148‑1 (MQ=255)
                                                                                                                                   ggAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTCGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTgcgc           <  2:2382286/148‑1 (MQ=255)
                                                                                                                                    gAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGcc          >  1:3480749/1‑148 (MQ=255)
                                                                                                                                      gCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAg        <  2:1277932/148‑1 (MQ=255)
                                                                                                                                       cGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgg       >  1:2844361/1‑148 (MQ=255)
                                                                                                                                       cGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgg       <  2:2624055/148‑1 (MQ=255)
                                                                                                                                       cGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgg       <  1:628495/148‑1 (MQ=255)
                                                                                                                                       cGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgg       >  2:1080652/1‑148 (MQ=255)
                                                                                                                                       cGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgg       >  2:1443276/1‑148 (MQ=255)
                                                                                                                                       cGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgg       >  2:2240769/1‑148 (MQ=255)
                                                                                                                                         gTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgggg     <  2:1196707/148‑1 (MQ=255)
                                                                                                                                         gTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAgggg     >  2:1701516/1‑148 (MQ=255)
                                                                                                                                          tttAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAggggg    >  1:3433020/1‑148 (MQ=255)
                                                                                                                                           ttAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGc   >  2:639258/1‑148 (MQ=255)
                                                                                                                                           ttAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGc   >  2:778056/1‑148 (MQ=255)
                                                                                                                                            tAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCg  <  2:1631983/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCG  >  CP009273/1451278‑1451565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATC  >  CP009273/1451274‑1451569
                                                                                                                                                    |                                                                                                                                                   
ATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTC                                                                                                                                                    <  SRR19973065.2375939/150‑1 (MQ=60)
ATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTC                                                                                                                                                    <  SRR19973065.601668/150‑1 (MQ=60)
     AGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTG                                                                                                                                               >  SRR19973065.1662902/1‑150 (MQ=60)
         ACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCAC                                                                                                                                           >  SRR19973065.7359/1‑150 (MQ=60)
            TGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCG                                                                                                                                        >  SRR19973065.1342177/1‑150 (MQ=60)
               GGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGT                                                                                                                                     >  SRR19973065.2015444/1‑150 (MQ=60)
                 CCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCA                                                                                                                                   >  SRR19973065.2154618/1‑150 (MQ=60)
                  CTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAA                                                                                                                                  >  SRR19973065.1207947/1‑150 (MQ=60)
                     GCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACG                                                                                                                               >  SRR19973065.2941134/1‑150 (MQ=60)
                     GCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACG                                                                                                                               <  SRR19973065.3325395/150‑1 (MQ=60)
                     GCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACG                                                                                                                               >  SRR19973065.2795332/1‑150 (MQ=60)
                      CTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGT                                                                                                                              <  SRR19973065.1004763/150‑1 (MQ=60)
                      CTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGT                                                                                                                              >  SRR19973065.1642937/1‑150 (MQ=60)
                      CTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGT                                                                                                                              >  SRR19973065.1641718/1‑150 (MQ=60)
                       TCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTA                                                                                                                             >  SRR19973065.417623/1‑150 (MQ=60)
                       TCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTA                                                                                                                             >  SRR19973065.417865/1‑150 (MQ=60)
                       TCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTA                                                                                                                             >  SRR19973065.417859/1‑150 (MQ=60)
                       TCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTA                                                                                                                             <  SRR19973065.794303/150‑1 (MQ=60)
                         ATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGC                                                                                                                           >  SRR19973065.2204250/1‑150 (MQ=60)
                         ATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGC                                                                                                                           >  SRR19973065.1204418/1‑150 (MQ=60)
                         ATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGC                                                                                                                           >  SRR19973065.2965251/1‑150 (MQ=60)
                         ATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGAGGTTTAATTAGCCAGGCACCCGCGTCAAACGTATC                                                                                                                           >  SRR19973065.1204426/1‑150 (MQ=60)
                            AATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTT                                                                                                                        <  SRR19973065.1256164/150‑1 (MQ=60)
                             ATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTA                                                                                                                       >  SRR19973065.243886/1‑150 (MQ=60)
                             ATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTA                                                                                                                       <  SRR19973065.1097842/150‑1 (MQ=60)
                                TTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACG                                                                                                                    >  SRR19973065.1204335/1‑150 (MQ=60)
                                  CGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTG                                                                                                                  >  SRR19973065.351054/1‑150 (MQ=60)
                                   GTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGC                                                                                                                 >  SRR19973065.833253/1‑150 (MQ=60)
                                     CTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAA                                                                                                               >  SRR19973065.2992867/1‑150 (MQ=60)
                                          TGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGA                                                                                                          >  SRR19973065.1286369/1‑150 (MQ=60)
                                          TGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGA                                                                                                          <  SRR19973065.1020408/150‑1 (MQ=60)
                                          TGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGA                                                                                                          <  SRR19973065.617293/150‑1 (MQ=60)
                                          TGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGA                                                                                                          <  SRR19973065.615114/150‑1 (MQ=60)
                                              GAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGA                                                                                                      <  SRR19973065.596274/150‑1 (MQ=60)
                                               AGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGAC                                                                                                     >  SRR19973065.3185225/1‑150 (MQ=60)
                                               AGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGAC                                                                                                     >  SRR19973065.3185278/1‑150 (MQ=60)
                                               AGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGAC                                                                                                     >  SRR19973065.3335721/1‑150 (MQ=60)
                                               AGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGAC                                                                                                     >  SRR19973065.3338634/1‑150 (MQ=60)
                                                GGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCGCTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACA                                                                                                    <  SRR19973065.977616/150‑1 (MQ=60)
                                                GGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACA                                                                                                    <  SRR19973065.3032664/150‑1 (MQ=60)
                                                   ATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAA                                                                                                 >  SRR19973065.3062047/1‑150 (MQ=60)
                                                   ATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAA                                                                                                 >  SRR19973065.3062359/1‑150 (MQ=60)
                                                   ATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAA                                                                                                 >  SRR19973065.3062374/1‑150 (MQ=60)
                                                   ATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAA                                                                                                 >  SRR19973065.238879/1‑150 (MQ=60)
                                                     TTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAG                                                                                               >  SRR19973065.359964/1‑150 (MQ=60)
                                                       ATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTG                                                                                             >  SRR19973065.2680417/1‑150 (MQ=60)
                                                       ATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTG                                                                                             >  SRR19973065.2679451/1‑150 (MQ=60)
                                                         TTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGAC                                                                                           <  SRR19973065.2034180/150‑1 (MQ=60)
                                                          TGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACT                                                                                          <  SRR19973065.2699142/150‑1 (MQ=60)
                                                          TGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACT                                                                                          <  SRR19973065.2764923/150‑1 (MQ=60)
                                                          TGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACT                                                                                          <  SRR19973065.744536/150‑1 (MQ=60)
                                                            TGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGT                                                                                        <  SRR19973065.2324693/150‑1 (MQ=60)
                                                              GTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGTACAAAAAGTGACTGTAC                                                                                      <  SRR19973065.674943/150‑1 (MQ=60)
                                                              GTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTAC                                                                                      <  SRR19973065.674895/150‑1 (MQ=60)
                                                              GTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTAC                                                                                      <  SRR19973065.3450584/150‑1 (MQ=60)
                                                               TCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACG                                                                                     <  SRR19973065.2761349/150‑1 (MQ=60)
                                                                  GGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCA                                                                                  >  SRR19973065.1975468/1‑150 (MQ=60)
                                                                      GCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGAT                                                                              <  SRR19973065.2422492/150‑1 (MQ=60)
                                                                      GCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGAT                                                                              <  SRR19973065.29811/150‑1 (MQ=60)
                                                                       CGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATG                                                                             <  SRR19973065.1608672/150‑1 (MQ=60)
                                                                       CGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATG                                                                             <  SRR19973065.2953999/150‑1 (MQ=60)
                                                                          TGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGC                                                                          <  SRR19973065.952574/150‑1 (MQ=60)
                                                                          TGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGC                                                                          <  SRR19973065.952777/150‑1 (MQ=60)
                                                                             TGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGG                                                                       <  SRR19973065.3068412/150‑1 (MQ=60)
                                                                              GGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGA                                                                      <  SRR19973065.2599446/150‑1 (MQ=60)
                                                                              GGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGA                                                                      <  SRR19973065.885342/150‑1 (MQ=60)
                                                                               GATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGAT                                                                     <  SRR19973065.1120790/150‑1 (MQ=60)
                                                                                ATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATC                                                                    <  SRR19973065.2043072/150‑1 (MQ=60)
                                                                                ATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATC                                                                    <  SRR19973065.2043221/150‑1 (MQ=60)
                                                                                     GCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAA                                                               <  SRR19973065.1168919/150‑1 (MQ=60)
                                                                                     GCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAA                                                               <  SRR19973065.1168916/150‑1 (MQ=60)
                                                                                      CGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAA                                                              >  SRR19973065.925261/1‑150 (MQ=60)
                                                                                       GGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAAT                                                             >  SRR19973065.3278576/1‑150 (MQ=60)
                                                                                       GGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAAT                                                             <  SRR19973065.2311092/150‑1 (MQ=60)
                                                                                          AACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGT                                                          >  SRR19973065.1411380/1‑150 (MQ=60)
                                                                                          AACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGT                                                          >  SRR19973065.866435/1‑150 (MQ=60)
                                                                                              GCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTT                                                      <  SRR19973065.2119454/150‑1 (MQ=60)
                                                                                              GCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTT                                                      <  SRR19973065.2113890/150‑1 (MQ=60)
                                                                                              GCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTT                                                      <  SRR19973065.2113922/150‑1 (MQ=60)
                                                                                              GCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTT                                                      <  SRR19973065.2113920/150‑1 (MQ=60)
                                                                                               CCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTA                                                     >  SRR19973065.1065733/1‑150 (MQ=60)
                                                                                               CCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTA                                                     <  SRR19973065.2662943/150‑1 (MQ=60)
                                                                                               CCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTA                                                     <  SRR19973065.2095029/150‑1 (MQ=60)
                                                                                               CCTTAAAAGCACTGGGAATGCCAGATAAAACCAGTCAACTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGAGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTA                                                     <  SRR19973065.2662613/150‑1 (MQ=60)
                                                                                                 TTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAAT                                                   <  SRR19973065.2320708/150‑1 (MQ=60)
                                                                                                    AAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAAGTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCC                                                <  SRR19973065.1782040/150‑1 (MQ=60)
                                                                                                     AAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCG                                               >  SRR19973065.3337578/1‑150 (MQ=60)
                                                                                                        CACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACG                                            >  SRR19973065.2360029/1‑150 (MQ=60)
                                                                                                          CTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGAT                                          <  SRR19973065.2552962/150‑1 (MQ=60)
                                                                                                              GAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAA                                      >  SRR19973065.3513743/1‑150 (MQ=60)
                                                                                                               AATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAG                                     <  SRR19973065.749448/150‑1 (MQ=60)
                                                                                                               AATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAG                                     <  SRR19973065.2521180/150‑1 (MQ=60)
                                                                                                               AATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAG                                     >  SRR19973065.296242/1‑150 (MQ=60)
                                                                                                               AATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAG                                     <  SRR19973065.2521194/150‑1 (MQ=60)
                                                                                                               AATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAG                                     >  SRR19973065.296251/1‑150 (MQ=60)
                                                                                                               AATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAG                                     <  SRR19973065.314031/150‑1 (MQ=60)
                                                                                                               AATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAG                                     <  SRR19973065.2095710/150‑1 (MQ=60)
                                                                                                                 TGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCA                                   >  SRR19973065.2149613/1‑150 (MQ=60)
                                                                                                                 TGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCA                                   >  SRR19973065.1363064/1‑150 (MQ=60)
                                                                                                                 TGCCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCA                                   >  SRR19973065.1363096/1‑150 (MQ=60)
                                                                                                                   CCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATT                                 >  SRR19973065.3474971/1‑150 (MQ=60)
                                                                                                                   CCAGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATT                                 >  SRR19973065.427423/1‑150 (MQ=60)
                                                                                                                     AGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCT                               >  SRR19973065.2654858/1‑150 (MQ=60)
                                                                                                                     AGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCT                               <  SRR19973065.2221070/150‑1 (MQ=60)
                                                                                                                     AGATAAAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCT                               >  SRR19973065.11603/1‑150 (MQ=60)
                                                                                                                         AAACCCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGAT                           <  SRR19973065.2252363/150‑1 (MQ=60)
                                                                                                                          AAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATG                          <  SRR19973065.425630/150‑1 (MQ=60)
                                                                                                                          AAACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATG                          >  SRR19973065.2327700/1‑150 (MQ=60)
                                                                                                                           AACCATTCATCTGGAGCGGTTTAATTAGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGAAGGACAAAAAGTGAATGTACGACAGGATGGGCGGGATCGGGAAATAGTGCTTAATGACGACGATGAAAGCATTCACGATGA                         >  SRR19973065.3045483/1‑150 (MQ=60)
                                                                                                                            ACCATTCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCG                        >  SRR19973065.1130035/1‑150 (MQ=60)
                                                                                                                                 TCATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATT                   >  SRR19973065.2382386/1‑150 (MQ=60)
                                                                                                                                   ATCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGC                 <  SRR19973065.2449873/150‑1 (MQ=60)
                                                                                                                                    TCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCG                <  SRR19973065.1475325/150‑1 (MQ=60)
                                                                                                                                    TCTGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCG                <  SRR19973065.1475495/150‑1 (MQ=60)
                                                                                                                                      TGGAGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCC              >  SRR19973065.3501931/1‑150 (MQ=60)
                                                                                                                                         AGCGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGG           >  SRR19973065.2861865/1‑150 (MQ=60)
                                                                                                                                           CGGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGG         <  SRR19973065.632589/150‑1 (MQ=60)
                                                                                                                                            GGTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGG        >  SRR19973065.3453970/1‑150 (MQ=60)
                                                                                                                                             GTTTAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGC       >  SRR19973065.2877727/1‑150 (MQ=60)
                                                                                                                                                TAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGA    >  SRR19973065.2799461/1‑150 (MQ=60)
                                                                                                                                                TAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGA    >  SRR19973065.2799514/1‑150 (MQ=60)
                                                                                                                                                TAATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGA    >  SRR19973065.1070645/1‑150 (MQ=60)
                                                                                                                                                  ATTCGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATC  >  SRR19973065.1846357/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCATCTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACGTCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGGATC  >  CP009273/1451274‑1451569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑