Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,477,565 A→T 54.3% K83I (AAA→ATA)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,477,5650AT54.3% 72.0 / 279.2 199K83I (AAA→ATA) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (50/41);  new base T (53/55);  total (103/96)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCG  >  CP009273/1477418‑1477712
                                                                                                                                                   |                                                                                                                                                   
tGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaaaa                                                                                                                                                     <  1:2495816/148‑1 (MQ=255)
tGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaaaa                                                                                                                                                     <  1:1550260/148‑1 (MQ=255)
  aagaagTTTAATCATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCTGTCGGCAGGGAAAGTCCTTCTGCGTGAAGCGGCACGACAGGTAATTAGTTATGCTGACAATTTTGCGGTTAGTCAGAAAATAc                                                                                                                                                   <  2:507884/148‑1 (MQ=255)
  aagaagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAAc                                                                                                                                                   <  1:2874121/148‑1 (MQ=255)
   agaagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACa                                                                                                                                                  >  2:1947410/1‑148 (MQ=255)
        gTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACa                                                                                                                                             <  2:3095135/148‑1 (MQ=255)
         ttAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACAt                                                                                                                                            <  2:182988/148‑1 (MQ=255)
         ttAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACAt                                                                                                                                            >  2:1420665/1‑148 (MQ=255)
          tAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACAtt                                                                                                                                           <  1:1387265/148‑1 (MQ=255)
           aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtc                                                                                                                                          <  1:3462814/148‑1 (MQ=255)
           aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtc                                                                                                                                          <  2:239534/148‑1 (MQ=255)
             aaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtctc                                                                                                                                        >  1:2372341/1‑148 (MQ=255)
                tCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGACATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGaa                                                                                                                                     >  2:1103939/1‑148 (MQ=255)
                   tGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCg                                                                                                                                  >  1:1057412/1‑148 (MQ=255)
                    gATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGa                                                                                                                                 <  1:882800/148‑1 (MQ=255)
                     aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAt                                                                                                                                <  2:2549987/148‑1 (MQ=255)
                      tGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAtt                                                                                                                               <  2:3417416/148‑1 (MQ=255)
                      tGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAtt                                                                                                                               >  1:150962/1‑148 (MQ=255)
                       gCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTc                                                                                                                              <  1:1856732/148‑1 (MQ=255)
                        cacaACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCg                                                                                                                             <  1:3185059/148‑1 (MQ=255)
                         acaacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGt                                                                                                                            >  1:1866275/1‑148 (MQ=255)
                          caacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTg                                                                                                                           >  2:3298374/1‑148 (MQ=255)
                           aacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGa                                                                                                                          >  2:2132682/1‑148 (MQ=255)
                           aacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGa                                                                                                                          >  1:2190955/1‑148 (MQ=255)
                             caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATc                                                                                                                        <  1:1608806/148‑1 (MQ=255)
                             caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATc                                                                                                                        <  1:442957/148‑1 (MQ=255)
                               ggCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCAc                                                                                                                      <  2:297477/148‑1 (MQ=255)
                               ggCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCAc                                                                                                                      <  2:308058/148‑1 (MQ=255)
                                 ccATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCa                                                                                                                    >  2:3283053/1‑148 (MQ=255)
                                  cATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAg                                                                                                                   <  1:469931/148‑1 (MQ=255)
                                   aTTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAgg                                                                                                                  >  1:1957472/1‑148 (MQ=255)
                                   aTTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAgg                                                                                                                  >  2:3175609/1‑148 (MQ=255)
                                      ttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtgg                                                                                                               <  1:1592573/148‑1 (MQ=255)
                                        ccAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGgtggtg                                                                                                             >  2:2682139/1‑148 (MQ=255)
                                            gtgaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcg                                                                                                         <  2:1199134/146‑1 (MQ=255)
                                            gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCGGACAATGTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcg                                                                                                         <  2:2492814/148‑1 (MQ=255)
                                             agaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgt                                                                                                        <  2:57239/148‑1 (MQ=255)
                                              gaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtc                                                                                                       >  2:3552463/1‑148 (MQ=255)
                                               aTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtcg                                                                                                      >  1:1139817/1‑148 (MQ=255)
                                               aTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtcg                                                                                                      >  2:1560967/1‑148 (MQ=255)
                                                   cGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCgg                                                                                                  >  1:2993447/1‑148 (MQ=255)
                                                        gagaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGaaa                                                                                             >  1:707095/1‑148 (MQ=255)
                                                        gagaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGaaa                                                                                             <  2:1769002/148‑1 (MQ=255)
                                                            ttGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACggg                                                                                         >  2:272130/1‑148 (MQ=255)
                                                             tGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGt                                                                                        >  1:1005729/1‑148 (MQ=255)
                                                               aCCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                      <  2:1021378/148‑1 (MQ=255)
                                                                  aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTgg                                                                                   <  1:1033070/148‑1 (MQ=255)
                                                                  aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTgg                                                                                   >  1:645743/1‑148 (MQ=255)
                                                                  aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTgg                                                                                   >  1:396575/1‑148 (MQ=255)
                                                                  aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTgg                                                                                   <  1:2670622/148‑1 (MQ=255)
                                                                      ggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaa                                                                               <  1:864371/148‑1 (MQ=255)
                                                                         aGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACg                                                                            >  1:1980322/1‑148 (MQ=255)
                                                                         aGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACg                                                                            >  1:2589000/1‑148 (MQ=255)
                                                                             aaaGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                                        >  2:3342832/1‑148 (MQ=255)
                                                                               aGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAg                                                                      >  1:75981/1‑148 (MQ=255)
                                                                                gTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGt                                                                     >  2:2283244/1‑148 (MQ=255)
                                                                                 tCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGtt                                                                    >  1:780296/1‑148 (MQ=255)
                                                                                 tCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGtt                                                                    >  2:518046/1‑148 (MQ=255)
                                                                                  ccTGCTGCGTGACGCGGCACGACGGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGACGCGTTTCGTGATCACCAGGTGGTTATGGTCGCGGGGGAAAGGGGTTCTGGTAAAACGACTCAGTTa                                                                   <  1:1778862/148‑1 (MQ=255)
                                                                                  ccTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGTTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAACCGGGTTCTGGTAAAACGACTCAGTTa                                                                   <  2:2773293/148‑1 (MQ=255)
                                                                                    tgctgcGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAcc                                                                 >  2:2332244/1‑148 (MQ=255)
                                                                                          gTGAAGCGGCACGACCGGAATTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGTTCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAAt                                                           <  2:1943069/148‑1 (MQ=255)
                                                                                            gAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCt                                                         >  2:3233037/1‑148 (MQ=255)
                                                                                            gAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAATCGACTCAGTTACCGAAAATCt                                                         >  1:1318392/1‑148 (MQ=255)
                                                                                            gAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCt                                                         >  1:1318946/1‑148 (MQ=255)
                                                                                             aaGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTg                                                        >  2:2505746/1‑148 (MQ=255)
                                                                                              aGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCGGGTAAAACGACTCAGTTACCGAAAATCTGt                                                       <  2:3259638/148‑1 (MQ=255)
                                                                                              aGCGCCACGCCCGGAAATTAATAATCCTGACAATTTCCCGGTTAGTCATAAAAAACAGGACTTTCTCGAAGCGATTCGTGAACACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGt                                                       <  1:258092/148‑1 (MQ=255)
                                                                                                    aCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGACGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAg                                                 <  2:3125068/148‑1 (MQ=255)
                                                                                                    aCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAg                                                 <  2:780296/148‑1 (MQ=255)
                                                                                                      gACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCt                                               >  1:2941508/1‑148 (MQ=255)
                                                                                                                  cTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAt                                   >  2:134095/1‑148 (MQ=255)
                                                                                                                   ttATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAtt                                  >  1:1973798/1‑148 (MQ=255)
                                                                                                                    tATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTAGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGACGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                                 >  2:2901244/1‑148 (MQ=255)
                                                                                                                    tATCCTCAAATTTTACGGGTTAGTCAGAAAATACTGGACATTCTCGAAGCGTTTCGTGATCACCTGGTGGTGATCGTGGCGGGGGAAAGGGGTTCTGGAAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                                 <  1:969167/148‑1 (MQ=255)
                                                                                                                      tCCTTAAAATTTACCGGTTAGTCATAAAATACAGTAATTTCTCGAAGCTATTCGTTATCACCAGGTGGTGATGGTCGCCGGGTAAACGGTTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                               <  1:1407707/148‑1 (MQ=255)
                                                                                                                      tCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGACGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                               <  2:1482052/148‑1 (MQ=255)
                                                                                                                      tCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                               >  1:3259451/1‑148 (MQ=255)
                                                                                                                         tGACAATTTACGGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGGGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                            <  2:79233/148‑1 (MQ=255)
                                                                                                                          gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc                           >  2:701498/1‑148 (MQ=255)
                                                                                                                            cAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGTTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACtc                         >  2:2008921/1‑147 (MQ=255)
                                                                                                                              aTTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGACGCGATTCGTGATCACCCGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGAt                       <  2:3315144/148‑1 (MQ=255)
                                                                                                                               tttACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATc                      >  1:2397621/1‑148 (MQ=255)
                                                                                                                                ttACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc                           >  2:1119044/1‑142 (MQ=255)
                                                                                                                                ttACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc                           <  1:1119044/142‑1 (MQ=255)
                                                                                                                                  acggtttAGACATAAAATACAGTAATTTCTCGATGCTATTCGTCATCACCTGGTGGTGATCGTCGCGGGGGAAAGGGGTTCTGTAAACACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGc                   <  1:3298256/143‑1 (MQ=255)
                                                                                                                                  aCCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGc                   >  1:1686386/1‑148 (MQ=255)
                                                                                                                                  aCCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGc                   >  1:3068366/1‑148 (MQ=255)
                                                                                                                                     ggTTAGTCAGAAAATACAGGACTTTCTCGAAGCGTTTGGTGATCACCCGGTGGTGATCGTCGCCGGGGAAAGGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAt                <  1:1103939/148‑1 (MQ=255)
                                                                                                                                      tttAGTAATAAAATACAGGAAATTCTCGAAGCGATTCGTTATCACCTGGTGGTGATCGTCGCCGGGTAAAGGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATa               <  1:1947410/147‑1 (MQ=255)
                                                                                                                                      gTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATa               >  1:528432/1‑148 (MQ=255)
                                                                                                                                      gTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATa               >  1:227641/1‑148 (MQ=255)
                                                                                                                                       ttAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAc              >  2:3436333/1‑148 (MQ=255)
                                                                                                                                        tAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAcc             >  2:383878/1‑148 (MQ=255)
                                                                                                                                            aataaaaaaCAGGAAATTCTCGACGCGATTCGTTATCACCTGGTGGTGATCGTCGCCGGGTAAACGGGTTCTGTAAACACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGc         <  1:2132682/145‑1 (MQ=255)
                                                                                                                                              gAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcg       >  2:1613822/1‑148 (MQ=255)
                                                                                                                                              gAAAAAACAGGACATTCTCGACGCGATTCGTGAACACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcg       <  1:1420665/148‑1 (MQ=255)
                                                                                                                                              gAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcg       >  2:650255/1‑148 (MQ=255)
                                                                                                                                                 aaaaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGcgt    <  1:2337843/148‑1 (MQ=255)
                                                                                                                                                  aaaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGcgtc   >  2:620994/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCG  >  CP009273/1477418‑1477712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A