Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,477,565 A→T 100% K83I (AAA→ATA)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,477,5650AT98.1% 645.8 / 1.4 211K83I (AAA→ATA) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base T (97/109);  total (98/113)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

AAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCG  >  CP009273/1477420‑1477709
                                                                                                                                                 |                                                                                                                                                
aagaagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATAc                                                                                                                                                >  1:1001651/1‑148 (MQ=255)
     ggTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGAc                                                                                                                                           >  1:167818/1‑148 (MQ=255)
       ttAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACAt                                                                                                                                         >  1:2046475/1‑148 (MQ=255)
        tAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACAtt                                                                                                                                        <  2:3119493/148‑1 (MQ=255)
         aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATtc                                                                                                                                       <  1:137974/148‑1 (MQ=255)
          aaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATtct                                                                                                                                      <  1:725894/148‑1 (MQ=255)
           aaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATtctc                                                                                                                                     >  1:455798/1‑148 (MQ=255)
             aTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGa                                                                                                                                   <  2:3372874/148‑1 (MQ=255)
               ccTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAg                                                                                                                                 >  1:703111/1‑148 (MQ=255)
                 tGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCg                                                                                                                               >  1:2955584/1‑148 (MQ=255)
                  gATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGa                                                                                                                              <  1:1100985/148‑1 (MQ=255)
                   aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAt                                                                                                                             <  1:1697500/148‑1 (MQ=255)
                   aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAt                                                                                                                             >  1:2484081/1‑148 (MQ=255)
                   aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAt                                                                                                                             <  2:3497311/148‑1 (MQ=255)
                   aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAt                                                                                                                             <  1:274612/148‑1 (MQ=255)
                   aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAt                                                                                                                             >  1:1698979/1‑148 (MQ=255)
                   aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAt                                                                                                                             <  2:2267539/148‑1 (MQ=255)
                     gCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTc                                                                                                                           >  2:2605130/1‑148 (MQ=255)
                      cacaACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCg                                                                                                                          >  2:386437/1‑148 (MQ=255)
                         aacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGa                                                                                                                       <  2:9413/148‑1 (MQ=255)
                           caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATc                                                                                                                     <  1:2605547/148‑1 (MQ=255)
                           caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATc                                                                                                                     <  1:1184247/148‑1 (MQ=255)
                              gCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCAcc                                                                                                                  >  1:3180402/1‑148 (MQ=255)
                              gCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCAcc                                                                                                                  <  2:359426/148‑1 (MQ=255)
                               ccATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCa                                                                                                                 >  2:303354/1‑148 (MQ=255)
                               ccATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAGTCGTGATCACCa                                                                                                                 >  1:2208294/1‑148 (MQ=255)
                                   tttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAggtg                                                                                                             <  1:2861081/148‑1 (MQ=255)
                                    ttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAggtgg                                                                                                            <  2:1389036/148‑1 (MQ=255)
                                       cAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGa                                                                                                         <  2:1880256/148‑1 (MQ=255)
                                       cAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGa                                                                                                         <  2:1880299/148‑1 (MQ=255)
                                          gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcg                                                                                                      <  2:1296727/148‑1 (MQ=255)
                                           agaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgt                                                                                                     >  1:2352627/1‑148 (MQ=255)
                                           agaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgt                                                                                                     <  2:1097097/148‑1 (MQ=255)
                                            gaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtc                                                                                                    >  2:1107669/1‑148 (MQ=255)
                                             aTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtcg                                                                                                   <  1:778583/148‑1 (MQ=255)
                                               ggCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGcc                                                                                                 <  1:3374204/148‑1 (MQ=255)
                                               ggCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGcc                                                                                                 <  2:1570851/148‑1 (MQ=255)
                                                gCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCg                                                                                                <  2:538393/148‑1 (MQ=255)
                                                  gAAAGAGATTGACCAGGCGGCAGGGACAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCggg                                                                                              <  1:981377/148‑1 (MQ=255)
                                                    aaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGa                                                                                            >  2:51644/1‑148 (MQ=255)
                                                    aaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGa                                                                                            >  2:1511095/1‑148 (MQ=255)
                                                         aTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACgg                                                                                       >  1:2284679/1‑148 (MQ=255)
                                                           tGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGt                                                                                     <  2:1891604/148‑1 (MQ=255)
                                                             aCCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                   <  1:666932/148‑1 (MQ=255)
                                                             aCCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                   <  2:901279/148‑1 (MQ=255)
                                                              ccAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCt                                                                                  <  2:2345810/148‑1 (MQ=255)
                                                               cAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTg                                                                                 <  2:3367601/148‑1 (MQ=255)
                                                                 ggcggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGt                                                                               <  1:1409428/148‑1 (MQ=255)
                                                                  gcggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTa                                                                              >  2:2150902/1‑148 (MQ=255)
                                                                  gcggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTa                                                                              >  2:2330054/1‑148 (MQ=255)
                                                                    ggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaa                                                                            <  2:2517521/148‑1 (MQ=255)
                                                                       aGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACg                                                                         <  1:996361/148‑1 (MQ=255)
                                                                        gggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                                        >  1:1089497/1‑148 (MQ=255)
                                                                         ggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGAc                                                                       >  1:1461934/1‑148 (MQ=255)
                                                                           aaaGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                                     >  1:2241765/1‑148 (MQ=255)
                                                                            aaGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCa                                                                    >  2:708859/1‑148 (MQ=255)
                                                                              gTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGt                                                                  <  2:2540549/148‑1 (MQ=255)
                                                                               tCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGtt                                                                 >  2:169063/1‑148 (MQ=255)
                                                                                ccTGCGGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCTAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTa                                                                <  2:1423109/148‑1 (MQ=255)
                                                                                     tgcGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaa                                                           >  2:1225664/1‑148 (MQ=255)
                                                                                             gCGGCACGACCGGAAATTACTTATCCTGACAATTTACGGGTTAGTCAGAAAATACAGGACTTTCTCGAAGCGTTTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                                   <  2:2791794/148‑1 (MQ=255)
                                                                                              cGGCACGACCGTAAATTACTTATCCTGACAATTTACCGTTTAGTCAGAAAATACCGGACATTCTCGAAGCGATTCTTGATCACCAGGTGGTGATCGTCGCCGGGTAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                                  <  2:801866/148‑1 (MQ=255)
                                                                                              cGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                                  >  2:1184001/1‑148 (MQ=255)
                                                                                              cGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                                  >  1:3466728/1‑148 (MQ=255)
                                                                                                  aCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTGGTGATCACCTGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAg                                              <  2:3477539/148‑1 (MQ=255)
                                                                                                   cGACCGTAAATTACTTATCCTGACAATTTACGGGTTAGTCATAAAATACAGGACATTCTCGACGCTATTCGTGAACACCTGGTGGTGATCGTCGCCGGGTAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGc                                             <  2:2696301/148‑1 (MQ=255)
                                                                                                      ccGTAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGACGCTATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgg                                          <  1:1739740/148‑1 (MQ=255)
                                                                                                        ggAAATTACTTATCCTGACAATTTAGCGGTTAGTCAGAAAATACAGGACTTTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgggg                                        <  2:1840903/148‑1 (MQ=255)
                                                                                                        ggAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgggg                                        <  2:2484081/148‑1 (MQ=255)
                                                                                                         gAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgc                                       >  2:2231039/1‑148 (MQ=255)
                                                                                                         gAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgc                                       >  1:3395184/1‑148 (MQ=255)
                                                                                                          aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcg                                      >  1:1788386/1‑148 (MQ=255)
                                                                                                          aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcg                                      >  1:40932/1‑148 (MQ=255)
                                                                                                          aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATGCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAACACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcg                                      <  2:2751930/148‑1 (MQ=255)
                                                                                                           aaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcgc                                     <  2:1699899/148‑1 (MQ=255)
                                                                                                           aaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcgc                                     >  2:1553505/1‑148 (MQ=255)
                                                                                                            aTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGAACACCCGGTGGTGATCGTCGCCGGGCAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGcgcg                                    <  1:2520036/148‑1 (MQ=255)
                                                                                                               aCTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGa                                 >  2:3438589/1‑148 (MQ=255)
                                                                                                                  tATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                              >  1:2627942/1‑148 (MQ=255)
                                                                                                                  tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                              >  1:1538994/1‑148 (MQ=255)
                                                                                                                  tATCCTCACAATTTACCGGTTCGACATAAAATACAGCACATTCTCCAAGCGATTCGTGACCACCAGGTGGTGATCGTCGCCGGGGAACCGGGTTCTGTTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                              <  1:2775401/148‑1 (MQ=255)
                                                                                                                    tCCTGACAATTTACCGGTTAGTCACAAAATACAGCACTTTCTCGAAGCGATTCGTGATCACCCGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGAAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                            <  1:1133885/148‑1 (MQ=255)
                                                                                                                    tCCTGAAATTTCACGGGTTAGTCATAAAATACAGGACTTTCTCGACGCGTTTGGTTATCACCTGGTGGTTATCGGCGCGGGGGAACGGGGTTCGGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                            <  1:1246473/148‑1 (MQ=255)
                                                                                                                       tGACATTTTACCGGTTAGTCAGAAAAAACAGGACAGTCTCGAAGCGATTCGTGATCACCTGGTGGTGATCGTCGCCGGGGAAAGGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                         <  2:2991410/148‑1 (MQ=255)
                                                                                                                       tGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                         <  2:3180402/148‑1 (MQ=255)
                                                                                                                        gACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc                        >  2:675391/1‑148 (MQ=255)
                                                                                                                         aCAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACt                       <  2:2955510/148‑1 (MQ=255)
                                                                                                                           aaTTTACCGTTTAGTCATAAAATACAGGACATTCTCTAAGCGTTTGGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGa                     <  1:1107669/148‑1 (MQ=255)
                                                                                                                                aCCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGCAACGGGTTCTGGTAAAACGACTCAGTTACCGACAATCTGTATGGAGCTGGGGCGCGGGATTAATGGACTGATCGGc                >  2:1781247/1‑148 (MQ=255)
                                                                                                                                aCCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGc                >  2:3011880/1‑148 (MQ=255)
                                                                                                                                  cGGTTTGTCACAAAATACAGGACATGCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGATACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCa              <  1:1931921/148‑1 (MQ=255)
                                                                                                                                  cGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCa              >  2:1922606/1‑148 (MQ=255)
                                                                                                                                   ggTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAt             >  1:3047655/1‑148 (MQ=255)
                                                                                                                                     ttAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAc           >  1:2248489/1‑148 (MQ=255)
                                                                                                                                     ttAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAc           >  1:2248937/1‑148 (MQ=255)
                                                                                                                                     ttAGTCAGAAAAGACAGGACATTCTCGACGCGATTCGTGATCACCCGGTGGTGATCGTCGCCGGGTAAACGGGGTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAc           <  2:2266722/148‑1 (MQ=255)
                                                                                                                                      tAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAcc          <  2:785699/148‑1 (MQ=255)
                                                                                                                                        gTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCa        >  1:3280590/1‑148 (MQ=255)
                                                                                                                                             aaaataCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcgc   >  2:1792575/1‑148 (MQ=255)
                                                                                                                                              aaataCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcgcg  >  1:3313592/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCG  >  CP009273/1477420‑1477709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTC  >  CP009273/1477416‑1477714
                                                                                                                                                     |                                                                                                                                                      
CGTGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAT                                                                                                                                                        >  SRR19973065.1522519/1‑150 (MQ=60)
  TGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATAC                                                                                                                                                      >  SRR19973065.1008302/1‑150 (MQ=60)
  TGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATAC                                                                                                                                                      >  SRR19973065.2843962/1‑150 (MQ=60)
    AAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAG                                                                                                                                                    >  SRR19973065.1711233/1‑150 (MQ=60)
     AGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGG                                                                                                                                                   >  SRR19973065.44369/1‑150 (MQ=60)
       AAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGAC                                                                                                                                                 >  SRR19973065.169180/1‑150 (MQ=60)
         GGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACAT                                                                                                                                               >  SRR19973065.2059562/1‑150 (MQ=60)
         GGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACAT                                                                                                                                               >  SRR19973065.2059342/1‑150 (MQ=60)
         GGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACAT                                                                                                                                               <  SRR19973065.39176/150‑1 (MQ=60)
           TTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTC                                                                                                                                             <  SRR19973065.1707745/150‑1 (MQ=60)
           TTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTC                                                                                                                                             <  SRR19973065.1389100/150‑1 (MQ=60)
             AAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTC                                                                                                                                           >  SRR19973065.1154330/1‑150 (MQ=60)
             AAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTC                                                                                                                                           <  SRR19973065.139159/150‑1 (MQ=60)
             AAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTC                                                                                                                                           >  SRR19973065.458948/1‑150 (MQ=60)
              AAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCG                                                                                                                                          <  SRR19973065.730758/150‑1 (MQ=60)
                AATCCTGATGCAAAACAGGCCATTTTCCATGAGATGGCGAAAGAGATTTACCAGGCGGCAGGGAAAGTCCTGCTGAGTGAAGCGGCACGAACGGAAATTACTTATCCTGACAATTTACCTGTTAGTCAGAAAATACAGGACATTCTCGAA                                                                                                                                        <  SRR19973065.2130005/150‑1 (MQ=60)
                 ATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAG                                                                                                                                       >  SRR19973065.707328/1‑150 (MQ=60)
                 ATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAG                                                                                                                                       >  SRR19973065.1511170/1‑150 (MQ=60)
                 ATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAG                                                                                                                                       >  SRR19973065.707786/1‑150 (MQ=60)
                   CCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCG                                                                                                                                     >  SRR19973065.2973545/1‑150 (MQ=60)
                   CCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCG                                                                                                                                     >  SRR19973065.2973620/1‑150 (MQ=60)
                   CCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCG                                                                                                                                     <  SRR19973065.3125308/150‑1 (MQ=60)
                     TGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAT                                                                                                                                   >  SRR19973065.2499713/1‑150 (MQ=60)
                     TGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAT                                                                                                                                   >  SRR19973065.1710308/1‑150 (MQ=60)
                      GATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATT                                                                                                                                  <  SRR19973065.1108220/150‑1 (MQ=60)
                       ATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTC                                                                                                                                 <  SRR19973065.276456/150‑1 (MQ=60)
                       ATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTC                                                                                                                                 <  SRR19973065.1708826/150‑1 (MQ=60)
                         GCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGT                                                                                                                               <  SRR19973065.1313372/150‑1 (MQ=60)
                             AACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATC                                                                                                                           <  SRR19973065.1455862/150‑1 (MQ=60)
                               CAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCAC                                                                                                                         <  SRR19973065.1191746/150‑1 (MQ=60)
                               CAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCAC                                                                                                                         <  SRR19973065.1011310/150‑1 (MQ=60)
                               CAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCAC                                                                                                                         <  SRR19973065.2621717/150‑1 (MQ=60)
                                AGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACC                                                                                                                        >  SRR19973065.3199622/1‑150 (MQ=60)
                                 GGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAGTCGTGATCACCA                                                                                                                       >  SRR19973065.2222416/1‑150 (MQ=60)
                                       TTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGT                                                                                                                 <  SRR19973065.2878649/150‑1 (MQ=60)
                                           CAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATC                                                                                                             <  SRR19973065.2533159/150‑1 (MQ=60)
                                             GGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGT                                                                                                           >  SRR19973065.2367266/1‑150 (MQ=60)
                                                 ATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCC                                                                                                       <  SRR19973065.518420/150‑1 (MQ=60)
                                                 ATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCC                                                                                                       <  SRR19973065.783755/150‑1 (MQ=60)
                                                   GGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGG                                                                                                     <  SRR19973065.3394840/150‑1 (MQ=60)
                                                   GGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGG                                                                                                     <  SRR19973065.3395080/150‑1 (MQ=60)
                                                   GGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGG                                                                                                     >  SRR19973065.929115/1‑150 (MQ=60)
                                                      GAAAGAGATTGACCAGGCGGCAGGGACAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGA                                                                                                  <  SRR19973065.987892/150‑1 (MQ=60)
                                                       AAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTAAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAA                                                                                                 <  SRR19973065.2638014/150‑1 (MQ=60)
                                                           AGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGG                                                                                             >  SRR19973065.2298159/1‑150 (MQ=60)
                                                           AGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGG                                                                                             >  SRR19973065.2299081/1‑150 (MQ=60)
                                                              TTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTT                                                                                          <  SRR19973065.722633/150‑1 (MQ=60)
                                                              TTGACCAGGCGGCAAGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTT                                                                                          <  SRR19973065.2496154/150‑1 (MQ=60)
                                                                 ACCAGGCGGCAGGGAAAGTCCTGCTGCGTGACGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTG                                                                                       <  SRR19973065.2440567/150‑1 (MQ=60)
                                                                 ACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTG                                                                                       <  SRR19973065.671372/150‑1 (MQ=60)
                                                                     GGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAA                                                                                   <  SRR19973065.1418591/150‑1 (MQ=60)
                                                                     GGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAA                                                                                   <  SRR19973065.1418648/150‑1 (MQ=60)
                                                                        GGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAAC                                                                                <  SRR19973065.2707061/150‑1 (MQ=60)
                                                                          CAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGA                                                                              >  SRR19973065.1096675/1‑150 (MQ=60)
                                                                          CAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGA                                                                              >  SRR19973065.1098430/1‑150 (MQ=60)
                                                                           AGGTAAAGTCCTGCTGCGTGAAGCGGCACGACCGTAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGAC                                                                             <  SRR19973065.1002984/150‑1 (MQ=60)
                                                                           AGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGAC                                                                             >  SRR19973065.1471328/1‑150 (MQ=60)
                                                                             GGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTC                                                                           >  SRR19973065.2256433/1‑150 (MQ=60)
                                                                             GGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTC                                                                           >  SRR19973065.2256008/1‑150 (MQ=60)
                                                                                 AGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTT                                                                       >  SRR19973065.1788821/1‑150 (MQ=60)
                                                                                 AGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTT                                                                       >  SRR19973065.3504184/1‑150 (MQ=60)
                                                                                 AGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTT                                                                       >  SRR19973065.1942327/1‑150 (MQ=60)
                                                                                  GTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTA                                                                      >  SRR19973065.2942333/1‑150 (MQ=60)
                                                                                   TCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAC                                                                     >  SRR19973065.3254619/1‑150 (MQ=60)
                                                                                      TGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑A                                                                 >  SRR19973065.2329805/1‑150 (MQ=60)
                                                                                          gggttacggggcacgaccgtaaatatcttatcgtgaaaatttagggtTTAGTCATAAAAAACAGGAAATTCTCGATGCGATTCGTGATCACCTGTGGGTTTTCTTCGCGGGGGAAACGTGTTCTGGTAAAACGACTCAGTTACCG‑AAAAT                                                             <  SRR19973065.1933533/103‑1 (MQ=47)
                                                                                             TGACGCGGCACGACCTTAATTTACTTATCCTCACAATTTACGGTTTAGTCATAAAATACAGTAAATTCTCTACGCGATTCGTTAACCCCGGGTGGTGTTGGTCGCGGGGTAAAGGGTTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTG                                                          <  SRR19973065.1583574/150‑1 (MQ=19)
                                                                                                cgcggcccgacggtAAATTAGTTATCCTCACATTTTACCGTTTAGTCACAAAAAACCGCACTTTCTCGACGCGATTGGTGAACACCAGGTGGTGATCGTCGCCGGGCAAAGGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTAT                                                       <  SRR19973065.2988421/136‑1 (MQ=60)
                                                                                                AGCGGCCCGACCGTAATTAACTTATCCTTACATTTGCCCGGTTAGACATAAAATACAGGACATTCCCGAAGCTATTCGTGATCACCGGGTGGTGATCGTGGCCGGGTAACGGGGTTCTGGTAACACGACTCAGTTACCG‑AAAATCTGTAT                                                       <  SRR19973065.2988404/150‑1 (MQ=50)
                                                                                                AGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTAT                                                       >  SRR19973065.3487839/1‑150 (MQ=60)
                                                                                                AGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTAT                                                       >  SRR19973065.781284/1‑150 (MQ=60)
                                                                                                     CACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGC                                                  >  SRR19973065.3448770/1‑150 (MQ=60)
                                                                                                          CCGTAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGACGCTATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGG                                             <  SRR19973065.1751230/150‑1 (MQ=60)
                                                                                                           cttaatttcattatccttaaaatttacggtttttgaatAAAAAACAGCAAATTCTCGACGCGATTCGTGATCACCTGGTGGTGATGGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGC                                            <  SRR19973065.1859294/112‑1 (MQ=60)
                                                                                                           CGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGC                                            >  SRR19973065.3415944/1‑150 (MQ=60)
                                                                                                           CGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGC                                            >  SRR19973065.994280/1‑150 (MQ=60)
                                                                                                            GGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCG                                           >  SRR19973065.1800099/1‑150 (MQ=60)
                                                                                                            GGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCG                                           >  SRR19973065.41430/1‑150 (MQ=60)
                                                                                                             GAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGAAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGC                                          >  SRR19973065.1709500/1‑150 (MQ=60)
                                                                                                                TTTACTTATCCTGACAATTTACCGGTTAGTCATAAAATACAGGACATTCTCGAAGCGATTCGTGAACACCCGGTGGTGATCGTCGCCGGGCAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGG                                       <  SRR19973065.2535859/150‑1 (MQ=60)
                                                                                                                   ACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACAGTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATT                                    >  SRR19973065.2246408/1‑150 (MQ=60)
                                                                                                                    CTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTA                                   >  SRR19973065.2644198/1‑150 (MQ=60)
                                                                                                                    CTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTA                                   >  SRR19973065.1549159/1‑150 (MQ=60)
                                                                                                                      TATCCTCACAATTTACCGGTTCGACATAAAATACAGCACATTCTCCAAGCGATTCGTGACCACCAGGTGGTGATCGTCGCCGGGGAACCGGGTTCTGTTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAA                                 <  SRR19973065.2792630/150‑1 (MQ=60)
                                                                                                                      TAGCCTGACAATTTCCCGGTTAGTCACAAAATCCCGGACAGACTCGACGCGATTCGTGAACACCCGGTGGTGATCGTCGCGGGGGAAAGGGGTTCTGGTAACACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAA                                 <  SRR19973065.2792053/150‑1 (MQ=60)
                                                                                                                        tcctgaaatttcacgGGTTAGTCATAAAATACAGGACTTTCTCGACGCGTTTGGTTATCACCTGGTGGTTATCGGCGCGGGGGAACGGGGTTCGGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGG                               <  SRR19973065.1254681/135‑1 (MQ=60)
                                                                                                                        TCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAG                                >  SRR19973065.2976701/1‑150 (MQ=60)
                                                                                                                        TCCTGACAATTTACCGGTTAGTCACAAAATACAGCACTTTCTCGAAGCGATTCGTGATCACCCGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGAAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGG                               <  SRR19973065.1141237/150‑1 (MQ=60)
                                                                                                                               AATTTACCGTTTAGTCATAAAATACAGGACATTCTCTAAGCGTTTGGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATC                        <  SRR19973065.1114924/150‑1 (MQ=60)
                                                                                                                                     CCGGTTAGTCAGAAAATACTGGACATTCTCGAAGCGATTCGTGATCACCCGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAT                  <  SRR19973065.2621300/150‑1 (MQ=60)
                                                                                                                                     CCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAT                  >  SRR19973065.3066304/1‑150 (MQ=60)
                                                                                                                                      CGGTTTGTCACAAAATACAGGACATGCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGATACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATA                 <  SRR19973065.1944571/150‑1 (MQ=60)
                                                                                                                                       GGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAC                >  SRR19973065.2262749/1‑150 (MQ=60)
                                                                                                                                       GGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAC                >  SRR19973065.2262760/1‑150 (MQ=60)
                                                                                                                                       GGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAC                >  SRR19973065.2263197/1‑150 (MQ=60)
                                                                                                                                          TAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCA             >  SRR19973065.3300539/1‑150 (MQ=60)
                                                                                                                                          TAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCA             >  SRR19973065.3300530/1‑150 (MQ=60)
                                                                                                                                              CAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCG         >  SRR19973065.1638732/1‑150 (MQ=60)
                                                                                                                                              CAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCG         >  SRR19973065.3386260/1‑150 (MQ=60)
                                                                                                                                                GAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCG       >  SRR19973065.3333793/1‑150 (MQ=60)
                                                                                                                                                  AAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAAagatcggaagagcacacgtctgaactccagtcactgatgccagaatctcggggggcg     >  SRR19973065.1473311/1‑93 (MQ=60)
                                                                                                                                                     TACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTC  >  SRR19973065.3449/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                      
CGTGAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG‑AAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTC  >  CP009273/1477416‑1477714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑