Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,478,971 G→A 100% E552K (GAG→AAG)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,478,9710GA95.6% 642.4 / 15.9 206E552K (GAG→AAG) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base A (100/97);  total (105/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 208 total aligned reads displayed.

CGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCC  >  CP009273/1478827‑1479115
                                                                                                                                                |                                                                                                                                                
cgTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGt                                                                                                                                               <  2:549695/148‑1 (MQ=255)
 gTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTc                                                                                                                                              <  1:120197/148‑1 (MQ=255)
   aTGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTg                                                                                                                                            >  2:755072/1‑148 (MQ=255)
    tGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGa                                                                                                                                           >  1:1724982/1‑148 (MQ=255)
    tGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGa                                                                                                                                           >  2:3526857/1‑148 (MQ=255)
     ggTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGAc                                                                                                                                          <  2:2800452/148‑1 (MQ=255)
     ggTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGAc                                                                                                                                          >  2:876988/1‑148 (MQ=255)
      gTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACt                                                                                                                                         <  2:3151817/148‑1 (MQ=255)
      gTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACt                                                                                                                                         <  2:81499/148‑1 (MQ=255)
          tGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTtct                                                                                                                                     <  1:2862595/148‑1 (MQ=255)
             aaGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTcgc                                                                                                                                  >  2:1308458/1‑148 (MQ=255)
                 gcAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGttt                                                                                                                              <  2:3350677/148‑1 (MQ=255)
                 gcAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGttt                                                                                                                              <  2:1586018/148‑1 (MQ=255)
                    aaaaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTtgtg                                                                                                                           >  1:2144156/1‑148 (MQ=255)
                     aaaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGa                                                                                                                          >  2:2736759/1‑148 (MQ=255)
                      aaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGaa                                                                                                                         >  2:1762930/1‑148 (MQ=255)
                      aaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGaa                                                                                                                         >  2:1192856/1‑148 (MQ=255)
                       aCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAAt                                                                                                                        >  1:225233/1‑148 (MQ=255)
                       aCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAAt                                                                                                                        >  1:225704/1‑148 (MQ=255)
                        cATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATc                                                                                                                       <  1:1291616/148‑1 (MQ=255)
                        cATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGGTTGTGAATc                                                                                                                       >  1:1909576/1‑148 (MQ=255)
                         aTGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCt                                                                                                                      <  1:1762930/148‑1 (MQ=255)
                              tgcgtgcgTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGa                                                                                                                 <  1:3526857/148‑1 (MQ=255)
                                 gtgcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAtt                                                                                                              <  1:3329777/148‑1 (MQ=255)
                                  tgcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTa                                                                                                             <  2:1022696/148‑1 (MQ=255)
                                   gcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAt                                                                                                            >  2:1987188/1‑148 (MQ=255)
                                    cgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATc                                                                                                           >  2:619877/1‑148 (MQ=255)
                                    cgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATc                                                                                                           >  2:100438/1‑148 (MQ=255)
                                      tgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCtt                                                                                                         <  1:2829875/148‑1 (MQ=255)
                                       gAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTTACTTTCTCGCGTTTGTGAATCGGTGGAATTATCTTg                                                                                                        >  1:1128848/1‑148 (MQ=255)
                                       gAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTg                                                                                                        >  1:1129035/1‑148 (MQ=255)
                                           cGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGa                                                                                                    >  1:2873870/1‑148 (MQ=255)
                                           cGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGa                                                                                                    <  1:310408/148‑1 (MQ=255)
                                            gatgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGCTGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGag                                                                                                   <  1:2366122/148‑1 (MQ=255)
                                            gatgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGag                                                                                                   <  1:441967/148‑1 (MQ=255)
                                             atgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGagc                                                                                                  <  1:768388/148‑1 (MQ=255)
                                             atgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTAGAATTATCTTGGCGagc                                                                                                  >  2:969442/1‑148 (MQ=255)
                                              tgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGagca                                                                                                 >  1:2496088/1‑148 (MQ=255)
                                               gatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGagcag                                                                                                >  1:341823/1‑148 (MQ=255)
                                                     cACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAg                                                                                          >  1:1446536/1‑148 (MQ=255)
                                                          ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCt                                                                                     >  1:1178321/1‑148 (MQ=255)
                                                          ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGACTTATCTTGGCGAGCAGCAAAAGGCGCt                                                                                     >  2:2773340/1‑148 (MQ=255)
                                                          ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCt                                                                                     <  2:1923651/148‑1 (MQ=255)
                                                          ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCt                                                                                     <  1:2736759/148‑1 (MQ=255)
                                                            gcgcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCttt                                                                                   >  1:2896846/1‑148 (MQ=255)
                                                            gcgcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCttt                                                                                   <  1:2042970/148‑1 (MQ=255)
                                                              gcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttct                                                                                 <  2:3127614/148‑1 (MQ=255)
                                                               ctctCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttctt                                                                                <  1:2672974/148‑1 (MQ=255)
                                                               ctctCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttctt                                                                                <  1:1689604/148‑1 (MQ=255)
                                                                tctcCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttcttc                                                                               >  2:385237/1‑148 (MQ=255)
                                                                tctcCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttcttc                                                                               >  2:385057/1‑148 (MQ=255)
                                                                tctcCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttcttc                                                                               <  2:1696343/148‑1 (MQ=255)
                                                                tctcCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttcttc                                                                               <  1:3492854/148‑1 (MQ=255)
                                                                     aTTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACg                                                                          <  1:1192856/148‑1 (MQ=255)
                                                                      ttCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGc                                                                         >  1:3474675/1‑148 (MQ=255)
                                                                       tCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGcc                                                                        <  2:316195/148‑1 (MQ=255)
                                                                         aGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCtt                                                                      >  2:2131353/1‑148 (MQ=255)
                                                                          ggATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTc                                                                     <  1:2422070/148‑1 (MQ=255)
                                                                          ggATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTc                                                                     <  1:1433552/148‑1 (MQ=255)
                                                                          ggATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAGTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTc                                                                     >  2:2086167/1‑148 (MQ=255)
                                                                           gATCCGCGCGAACGTCAGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTcc                                                                    >  2:1942901/1‑148 (MQ=255)
                                                                            aTCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCg                                                                   >  1:460986/1‑148 (MQ=255)
                                                                             tCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAGTATCTTGGCGAGCAGCCAAAGGCGCTTTCTTCCAACGCCTTCCGt                                                                  >  1:2225221/1‑148 (MQ=255)
                                                                                 cgcgAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGcc                                                              >  2:106913/1‑148 (MQ=255)
                                                                                     aaCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCtgtg                                                          >  2:1227850/1‑148 (MQ=255)
                                                                                     aaCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCtgtg                                                          >  2:224867/1‑148 (MQ=255)
                                                                                      aCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTgtgt                                                         <  1:1455909/148‑1 (MQ=255)
                                                                                      aCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTgtgt                                                         >  2:888382/1‑148 (MQ=255)
                                                                                      aCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTACACGACAAAAAGTCTGACTTTATCGGGTTTTTGAATCTGTGGAAATATCTTTGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTgtgt                                                         >  1:1542290/1‑148 (MQ=255)
                                                                                         tCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGt                                                      <  1:3206181/148‑1 (MQ=255)
                                                                                         tCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGt                                                      >  2:2079669/1‑148 (MQ=255)
                                                                                          ccGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTa                                                     >  1:3293659/1‑148 (MQ=255)
                                                                                           cGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTAc                                                    <  2:2526939/148‑1 (MQ=255)
                                                                                              tGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGa                                                 <  1:634571/148‑1 (MQ=255)
                                                                                                  cAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTAt                                             <  2:2741752/148‑1 (MQ=255)
                                                                                                       agcagGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCaa                                        <  2:1538959/148‑1 (MQ=255)
                                                                                                       agcagGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCaa                                        <  1:3007894/148‑1 (MQ=255)
                                                                                                          agGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTa                                     <  2:3488160/148‑1 (MQ=255)
                                                                                                               tCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgc                                >  1:1869521/1‑148 (MQ=255)
                                                                                                                cGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcg                               <  2:225233/148‑1 (MQ=255)
                                                                                                                cGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcg                               <  2:225704/148‑1 (MQ=255)
                                                                                                                cGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcg                               >  1:478061/1‑148 (MQ=255)
                                                                                                                    cGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcgcgtg                           <  1:877095/148‑1 (MQ=255)
                                                                                                                    cGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcgcgtg                           <  1:673749/148‑1 (MQ=255)
                                                                                                                      aaaaaCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcgcgtgcg                         <  1:112632/148‑1 (MQ=255)
                                                                                                                         aaCATCGTCGCTTCCAAGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAAAGCCTTCAGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGa                      >  2:1046043/1‑148 (MQ=255)
                                                                                                                          aCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGaa                     <  2:785759/148‑1 (MQ=255)
                                                                                                                              cgtcgCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGc                 >  2:1825298/1‑148 (MQ=255)
                                                                                                                              cgtcgCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGc                 <  2:59025/148‑1 (MQ=255)
                                                                                                                                 cgCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAgg              >  1:279728/1‑148 (MQ=255)
                                                                                                                                   cTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAGTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGat            <  2:2654925/148‑1 (MQ=255)
                                                                                                                                     tCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGatat          >  1:1106760/1‑148 (MQ=255)
                                                                                                                                     tCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGatat          >  2:2449414/1‑148 (MQ=255)
                                                                                                                                      ccACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATc         >  2:509374/1‑148 (MQ=255)
                                                                                                                                      ccACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATc         <  2:1768624/148‑1 (MQ=255)
                                                                                                                                        aCGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTa       >  2:440126/1‑148 (MQ=255)
                                                                                                                                         cGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTac      <  1:1162541/148‑1 (MQ=255)
                                                                                                                                            cAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACAcc   >  1:2928096/1‑148 (MQ=255)
                                                                                                                                            cAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACAcc   >  2:2100131/1‑148 (MQ=255)
                                                                                                                                             aaaaAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACAccc  >  1:848518/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCC  >  CP009273/1478827‑1479115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTT  >  CP009273/1478822‑1479119
                                                                                                                                                     |                                                                                                                                                    
TGGCGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAA                                                                                                                                                      >  SRR19973066.920878/1‑150 (MQ=60)
TGGCGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAA                                                                                                                                                      >  SRR19973066.2780650/1‑150 (MQ=60)
TGGCGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAA                                                                                                                                                      >  SRR19973066.2650519/1‑150 (MQ=60)
   CGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGT                                                                                                                                                   >  SRR19973066.59757/1‑150 (MQ=60)
   CGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGT                                                                                                                                                   >  SRR19973066.2477190/1‑150 (MQ=60)
   CGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGT                                                                                                                                                   >  SRR19973066.2317324/1‑150 (MQ=60)
      GTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTG                                                                                                                                                <  SRR19973066.121414/150‑1 (MQ=60)
       TATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGA                                                                                                                                               >  SRR19973066.1737463/1‑150 (MQ=60)
       TATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGA                                                                                                                                               <  SRR19973066.334910/150‑1 (MQ=60)
         TGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACT                                                                                                                                             <  SRR19973066.1158168/150‑1 (MQ=60)
          GGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTT                                                                                                                                            >  SRR19973066.183045/1‑150 (MQ=60)
             GCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCT                                                                                                                                         <  SRR19973066.1445508/150‑1 (MQ=60)
              CTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTC                                                                                                                                        <  SRR19973066.1397745/150‑1 (MQ=60)
               TGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCG                                                                                                                                       <  SRR19973066.2881797/150‑1 (MQ=60)
               TGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCG                                                                                                                                       <  SRR19973066.2881815/150‑1 (MQ=60)
                       CAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTG                                                                                                                               <  SRR19973066.364197/150‑1 (MQ=60)
                       CAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTG                                                                                                                               >  SRR19973066.2159005/1‑150 (MQ=60)
                       CAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTG                                                                                                                               >  SRR19973066.2159041/1‑150 (MQ=60)
                          AAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAAT                                                                                                                            >  SRR19973066.227520/1‑150 (MQ=60)
                          AAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAAT                                                                                                                            >  SRR19973066.227049/1‑150 (MQ=60)
                           AACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATC                                                                                                                           >  SRR19973066.1922858/1‑150 (MQ=60)
                           AACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGGTTGTGAATC                                                                                                                           >  SRR19973066.1923177/1‑150 (MQ=60)
                             CATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTG                                                                                                                         <  SRR19973066.1300966/150‑1 (MQ=60)
                              ATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGT                                                                                                                        <  SRR19973066.1775579/150‑1 (MQ=60)
                              ATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGT                                                                                                                        <  SRR19973066.1775500/150‑1 (MQ=60)
                                   TGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAT                                                                                                                   <  SRR19973066.3550323/150‑1 (MQ=60)
                                   TGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAT                                                                                                                   <  SRR19973066.3550324/150‑1 (MQ=60)
                                      GTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAT                                                                                                                <  SRR19973066.3351903/150‑1 (MQ=60)
                                      GTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAT                                                                                                                <  SRR19973066.3351961/150‑1 (MQ=60)
                                       TGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATC                                                                                                               <  SRR19973066.1202838/150‑1 (MQ=60)
                                         CGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTT                                                                                                             <  SRR19973066.291658/150‑1 (MQ=60)
                                          GTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTTACTTTCTCGCGTTTGTGAATCGGTGGAATTATCTTG                                                                                                            >  SRR19973066.1136954/1‑150 (MQ=60)
                                          GTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTG                                                                                                            >  SRR19973066.1136892/1‑150 (MQ=60)
                                          GTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTG                                                                                                            >  SRR19973066.1137142/1‑150 (MQ=60)
                                          GTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTG                                                                                                            >  SRR19973066.1137075/1‑150 (MQ=60)
                                           TGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGG                                                                                                           <  SRR19973066.2848855/150‑1 (MQ=60)
                                           TGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGG                                                                                                           <  SRR19973066.2848891/150‑1 (MQ=60)
                                              GGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGA                                                                                                        >  SRR19973066.2893136/1‑150 (MQ=60)
                                                CGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGC                                                                                                      >  SRR19973066.464077/1‑150 (MQ=60)
                                                CGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGC                                                                                                      >  SRR19973066.2268781/1‑150 (MQ=60)
                                                CGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGC                                                                                                      <  SRR19973066.312680/150‑1 (MQ=60)
                                                CGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGC                                                                                                      <  SRR19973066.3179325/150‑1 (MQ=60)
                                                 GATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGCTGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCA                                                                                                     <  SRR19973066.2382141/150‑1 (MQ=60)
                                                 GATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCA                                                                                                     <  SRR19973066.445382/150‑1 (MQ=60)
                                                 GATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCA                                                                                                     >  SRR19973066.2513100/1‑150 (MQ=60)
                                                  ATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAG                                                                                                    <  SRR19973066.774080/150‑1 (MQ=60)
                                                  ATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAG                                                                                                    >  SRR19973066.344464/1‑150 (MQ=60)
                                                  ATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAG                                                                                                    <  SRR19973066.773914/150‑1 (MQ=60)
                                                      TTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTATCGCGGTTGTGAATCTGGGGAAGTATCTTGGCGAGCAGCAAA                                                                                                >  SRR19973066.3013876/1‑150 (MQ=60)
                                                        ATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAG                                                                                              >  SRR19973066.2254617/1‑150 (MQ=60)
                                                        ATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAG                                                                                              >  SRR19973066.1456819/1‑150 (MQ=60)
                                                         TCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGG                                                                                             >  SRR19973066.2952558/1‑150 (MQ=60)
                                                             GTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCT                                                                                         >  SRR19973066.1186616/1‑150 (MQ=60)
                                                             GTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCT                                                                                         >  SRR19973066.2883859/1‑150 (MQ=60)
                                                               CCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTT                                                                                       <  SRR19973066.2755164/150‑1 (MQ=60)
                                                               CCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTT                                                                                       >  SRR19973066.2916208/1‑150 (MQ=60)
                                                               CCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTT                                                                                       >  SRR19973066.2916217/1‑150 (MQ=60)
                                                                 GCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCT                                                                                     <  SRR19973066.2057189/150‑1 (MQ=60)
                                                                    CTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCC                                                                                  <  SRR19973066.1701900/150‑1 (MQ=60)
                                                                    CTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCC                                                                                  <  SRR19973066.2690834/150‑1 (MQ=60)
                                                                    CTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCC                                                                                  <  SRR19973066.2690743/150‑1 (MQ=60)
                                                                     TCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAACCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCA                                                                                 <  SRR19973066.3317957/150‑1 (MQ=60)
                                                                     TCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCA                                                                                 <  SRR19973066.3516136/150‑1 (MQ=60)
                                                                     TCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCA                                                                                 <  SRR19973066.3516150/150‑1 (MQ=60)
                                                                      CTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAA                                                                                >  SRR19973066.1243226/1‑150 (MQ=60)
                                                                        CCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACG                                                                              <  SRR19973066.1651001/150‑1 (MQ=60)
                                                                         CATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGC                                                                             >  SRR19973066.3496681/1‑150 (MQ=60)
                                                                         CATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGC                                                                             >  SRR19973066.3497877/1‑150 (MQ=60)
                                                                          ATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCC                                                                            >  SRR19973066.1588872/1‑150 (MQ=60)
                                                                          ATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCC                                                                            <  SRR19973066.1201201/150‑1 (MQ=60)
                                                                               GGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCG                                                                       <  SRR19973066.2438509/150‑1 (MQ=60)
                                                                               GGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCG                                                                       <  SRR19973066.2437358/150‑1 (MQ=60)
                                                                               GGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCG                                                                       <  SRR19973066.661253/150‑1 (MQ=60)
                                                                               GGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCG                                                                       <  SRR19973066.1443779/150‑1 (MQ=60)
                                                                               GGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCG                                                                       <  SRR19973066.872501/150‑1 (MQ=60)
                                                                               GGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCG                                                                       <  SRR19973066.2462054/150‑1 (MQ=60)
                                                                               GGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCG                                                                       >  SRR19973066.464513/1‑150 (MQ=60)
                                                                               GGATCCGAGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCG                                                                       <  SRR19973066.2748567/150‑1 (MQ=60)
                                                                                GATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAGTATCTTGGCGAGCAGCCAAAGGCGCTTTCTTCCAACGCCTTCCGT                                                                      >  SRR19973066.2240602/1‑150 (MQ=60)
                                                                                         GAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTACACGACAAAAAGTCTGACTTTATCGGGTTTTTGAATCTGTGGAAATATCTTTGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGT                                                             >  SRR19973066.1553320/1‑150 (MQ=60)
                                                                                           ACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCG                                                           <  SRR19973066.1466226/150‑1 (MQ=60)
                                                                                            CGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGT                                                          >  SRR19973066.2227555/1‑150 (MQ=60)
                                                                                            CGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGT                                                          >  SRR19973066.1116322/1‑150 (MQ=60)
                                                                                             GTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTA                                                         >  SRR19973066.3315400/1‑150 (MQ=60)
                                                                                              TCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTAC                                                        <  SRR19973066.3227451/150‑1 (MQ=60)
                                                                                              TCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTATGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTAC                                                        <  SRR19973066.3227534/150‑1 (MQ=60)
                                                                                                 GATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATGTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCCA                                                     >  SRR19973066.2776673/1‑150 (MQ=60)
                                                                                                   TGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATT                                                   <  SRR19973066.639139/150‑1 (MQ=60)
                                                                                                   TGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATT                                                   >  SRR19973066.1460145/1‑150 (MQ=60)
                                                                                                       CAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGGCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCCATTATCT                                               >  SRR19973066.1468295/1‑150 (MQ=60)
                                                                                                        AAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTC                                              >  SRR19973066.1940645/1‑150 (MQ=60)
                                                                                                            AGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACT                                          <  SRR19973066.3027908/150‑1 (MQ=60)
                                                                                                                  CATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGC                                    >  SRR19973066.1882875/1‑150 (MQ=60)
                                                                                                                   ATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCG                                   >  SRR19973066.481665/1‑150 (MQ=60)
                                                                                                                         CGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCG                             <  SRR19973066.883242/150‑1 (MQ=60)
                                                                                                                         CGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCG                             <  SRR19973066.678683/150‑1 (MQ=60)
                                                                                                                         CGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCG                             <  SRR19973066.883349/150‑1 (MQ=60)
                                                                                                                           AAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCG                           <  SRR19973066.113780/150‑1 (MQ=60)
                                                                                                                              AACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCATCCGTCGCCTGTGTCGTACCGATTATATCAACTATCTGCGCGTGCGCGAAT                        >  SRR19973066.47869/1‑150 (MQ=60)
                                                                                                                                    GTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGG                  >  SRR19973066.3100830/1‑150 (MQ=60)
                                                                                                                                    GTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGG                  >  SRR19973066.281765/1‑150 (MQ=60)
                                                                                                                                    GTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATATTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGG                  >  SRR19973066.986208/1‑150 (MQ=60)
                                                                                                                                       GCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATA               >  SRR19973066.1709860/1‑150 (MQ=60)
                                                                                                                                        CTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATAT              >  SRR19973066.1114775/1‑150 (MQ=60)
                                                                                                                                          TCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCT            >  SRR19973066.119501/1‑150 (MQ=60)
                                                                                                                                              CGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCACCTATCTGCGCGTGCGCGAATGGCAGGATATCTACAC        <  SRR19973066.1170792/150‑1 (MQ=60)
                                                                                                                                              CGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACAC        <  SRR19973066.1170786/150‑1 (MQ=60)
                                                                                                                                               GACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACC       >  SRR19973066.2947595/1‑150 (MQ=60)
                                                                                                                                                ACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGTCGCTTTCTTCCAACGCCTTCCGACGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCC      >  SRR19973066.854653/1‑150 (MQ=60)
                                                                                                                                                ACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCC      >  SRR19973066.854664/1‑150 (MQ=60)
                                                                                                                                                 CAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCA     >  SRR19973066.302885/1‑150 (MQ=60)
                                                                                                                                                 CAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCA     >  SRR19973066.1302526/1‑150 (MQ=60)
                                                                                                                                                    AAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTATTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTT  <  SRR19973066.624268/150‑1 (MQ=60)
                                                                                                                                                    AAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTATTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTT  <  SRR19973066.101550/150‑1 (MQ=60)
                                                                                                                                                    AAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTATTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTT  <  SRR19973066.101507/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TGGCGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTT  >  CP009273/1478822‑1479119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑