Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,478,971 G→A E552K (GAG→AAG)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,478,9710GA99.6% 793.3 / NA 240E552K (GAG→AAG) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (115/124);  minor base C (1/0);  total (116/124)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GCGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCA  >  CP009273/1478824‑1479116
                                                                                                                                                   |                                                                                                                                                 
gcgcGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAaaag                                                                                                                                                 <  2:850452/150‑1 (MQ=255)
    gTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTg                                                                                                                                             >  2:1517358/1‑150 (MQ=255)
     tATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGa                                                                                                                                            <  2:2157952/150‑1 (MQ=255)
       tGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACt                                                                                                                                          <  2:3597252/150‑1 (MQ=255)
       tGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACt                                                                                                                                          <  2:3598255/150‑1 (MQ=255)
        ggTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACtt                                                                                                                                         >  1:2841179/1‑150 (MQ=255)
         gTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACttt                                                                                                                                        <  1:2333865/150‑1 (MQ=255)
         gTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACttt                                                                                                                                        <  2:415410/150‑1 (MQ=255)
         gTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACttt                                                                                                                                        <  1:129206/150‑1 (MQ=255)
          tGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTtc                                                                                                                                       <  1:290256/150‑1 (MQ=255)
            cTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTtctc                                                                                                                                     <  1:2236459/150‑1 (MQ=255)
                 aGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGtt                                                                                                                                >  2:559442/1‑150 (MQ=255)
                 aGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGtt                                                                                                                                >  1:2877968/1‑150 (MQ=255)
                 aGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGtg                                                                                                                                >  2:2086998/1‑149 (MQ=255)
                  gcgcAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGttt                                                                                                                               <  1:3575510/150‑1 (MQ=255)
                  gcgcAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGttt                                                                                                                               <  2:970289/150‑1 (MQ=255)
                    gcAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTtgt                                                                                                                             <  2:1222827/150‑1 (MQ=255)
                     cAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTtgtg                                                                                                                            <  2:2819987/150‑1 (MQ=255)
                      aaaaaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGa                                                                                                                           >  1:3174998/1‑150 (MQ=255)
                      aaaaaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGa                                                                                                                           >  2:635210/1‑150 (MQ=255)
                      aaaaaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGa                                                                                                                           >  1:3022013/1‑150 (MQ=255)
                      aaaaaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGa                                                                                                                           >  2:1817198/1‑150 (MQ=255)
                          aCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCt                                                                                                                       <  1:550711/150‑1 (MQ=255)
                          aCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCt                                                                                                                       <  1:2923946/150‑1 (MQ=255)
                              ggCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTgg                                                                                                                   >  2:868317/1‑150 (MQ=255)
                               gCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGa                                                                                                                  >  1:1799109/1‑150 (MQ=255)
                               gCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGa                                                                                                                  <  1:1086374/150‑1 (MQ=255)
                                cTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGaa                                                                                                                 <  1:3721922/150‑1 (MQ=255)
                                 tgcgtgcgTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAt                                                                                                                <  2:3347884/150‑1 (MQ=255)
                                 tgcgtgcgTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTGTGTGAATCTGTGGaag                                                                                                                >  2:3302799/1‑149 (MQ=255)
                                   cgtgcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTa                                                                                                              <  1:2192501/150‑1 (MQ=255)
                                    gtgcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAt                                                                                                             <  1:2659081/150‑1 (MQ=255)
                                    gtgcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAt                                                                                                             <  2:428917/150‑1 (MQ=255)
                                    gtgcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGAGCGAACGTCCGATGGACAAACAGCAGGCATCGGACAAAAAACATCGACGCTTCCACGACAAAATTTATTACATTCTCGCGTTTGTTAATCTGTGGAATTAt                                                                                                             >  1:1970106/1‑150 (MQ=255)
                                    gtgcgtgAGGCGATGATCATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAt                                                                                                             <  1:2402109/150‑1 (MQ=255)
                                      gcgtgAGGCGATGTTTATCATGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCTGATGGACAAACATCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCt                                                                                                           <  1:720128/150‑1 (MQ=255)
                                         tgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTgg                                                                                                        <  2:1748736/150‑1 (MQ=255)
                                          gAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGc                                                                                                       >  2:733564/1‑150 (MQ=255)
                                             gCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGag                                                                                                    >  1:2464408/1‑150 (MQ=255)
                                              cGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGACTTATCTTGGCGagc                                                                                                   >  2:2665734/1‑150 (MQ=255)
                                              cGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGACTTATCTTGGCGagc                                                                                                   >  2:2666167/1‑150 (MQ=255)
                                               gatgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGagca                                                                                                  <  2:2422936/150‑1 (MQ=255)
                                                atgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGagcag                                                                                                 >  1:3706376/1‑150 (MQ=255)
                                                atgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGagcag                                                                                                 <  2:459124/150‑1 (MQ=255)
                                                atgatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGagcag                                                                                                 <  2:245222/150‑1 (MQ=255)
                                                  gatTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAgcagca                                                                                               <  2:3463999/150‑1 (MQ=255)
                                                   atTATCACGTCCGCGCTCTCCTTTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaa                                                                                              <  1:1307474/150‑1 (MQ=255)
                                                   atTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaa                                                                                              >  1:1996796/1‑150 (MQ=255)
                                                   atTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaa                                                                                              >  2:446852/1‑150 (MQ=255)
                                                   atTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaa                                                                                              <  1:2419335/150‑1 (MQ=255)
                                                   atTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaa                                                                                              <  1:225313/150‑1 (MQ=255)
                                                   atTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaa                                                                                              <  1:3295860/150‑1 (MQ=255)
                                                         aCGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGgcg                                                                                        <  2:1234486/150‑1 (MQ=255)
                                                         aCGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGgcg                                                                                        <  2:469845/150‑1 (MQ=255)
                                                         aCGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGgcg                                                                                        >  1:3005404/1‑150 (MQ=255)
                                                             ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCttt                                                                                    <  2:1412437/150‑1 (MQ=255)
                                                              cgcgCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttc                                                                                   >  2:3195721/1‑150 (MQ=255)
                                                                 gcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttcttc                                                                                >  2:311715/1‑150 (MQ=255)
                                                                  ctctCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTcc                                                                               >  1:2630302/1‑150 (MQ=255)
                                                                   tctcCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCa                                                                              <  2:3174998/150‑1 (MQ=255)
                                                                      ccATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACg                                                                           <  2:932360/150‑1 (MQ=255)
                                                                        aTTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGcc                                                                         >  2:2292460/1‑150 (MQ=255)
                                                                              gATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGt                                                                   >  1:949127/1‑150 (MQ=255)
                                                                               aTCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTc                                                                  <  2:3608919/150‑1 (MQ=255)
                                                                                  cgcgcgAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGcc                                                               <  2:1352072/150‑1 (MQ=255)
                                                                                     gcgAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCtgt                                                            <  1:1649143/150‑1 (MQ=255)
                                                                                        aaCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTc                                                         >  2:1550918/1‑150 (MQ=255)
                                                                                          cGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGt                                                       >  1:2088434/1‑150 (MQ=255)
                                                                                           gTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTa                                                      >  1:322728/1‑150 (MQ=255)
                                                                                              cGATGGACAAACAGCAGTCATCGGACGAAAAACATCGTCTCTTCCACGTCAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTACAACGCCTTCCGTCGCCTGTGTCGTACCg                                                   <  2:2593050/150‑1 (MQ=255)
                                                                                              cGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCg                                                   >  2:37473/1‑150 (MQ=255)
                                                                                                   gACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTAt                                              <  2:2877968/150‑1 (MQ=255)
                                                                                                   gACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTAt                                              <  2:3441599/150‑1 (MQ=255)
                                                                                                      aaaCAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTAtctc                                           >  1:861760/1‑150 (MQ=255)
                                                                                                          agcagGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACt                                       >  2:711528/1‑150 (MQ=255)
                                                                                                             agGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATc                                    >  1:1009998/1‑150 (MQ=255)
                                                                                                              ggCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCt                                   <  1:3319191/150‑1 (MQ=255)
                                                                                                               gCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtg                                  >  1:1060925/1‑150 (MQ=255)
                                                                                                               gCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtg                                  >  2:3008172/1‑150 (MQ=255)
                                                                                                               gCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtg                                  <  1:3796630/150‑1 (MQ=255)
                                                                                                                cATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgc                                 >  1:3371393/1‑150 (MQ=255)
                                                                                                                cATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgc                                 >  1:1474076/1‑150 (MQ=255)
                                                                                                                 aTCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcg                                >  1:2552668/1‑150 (MQ=255)
                                                                                                                 aTCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcg                                <  2:2942375/150‑1 (MQ=255)
                                                                                                                 aTCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcg                                <  2:1305937/150‑1 (MQ=255)
                                                                                                                        gAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcgcgtgcgc                         <  2:758016/150‑1 (MQ=255)
                                                                                                                         aaaaaCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcgcgtgcgcg                        >  1:1415039/1‑150 (MQ=255)
                                                                                                                           aaaCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGaa                      >  2:2597162/1‑150 (MQ=255)
                                                                                                                           aaaCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGaa                      >  2:690148/1‑150 (MQ=255)
                                                                                                                            aaCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAAt                     <  1:1817198/150‑1 (MQ=255)
                                                                                                                            aaCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAAt                     <  1:635210/150‑1 (MQ=255)
                                                                                                                               aTCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGc                  <  1:756438/150‑1 (MQ=255)
                                                                                                                                     gCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAgg               >  1:2792455/1‑147 (MQ=255)
                                                                                                                                     gCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAgg               <  2:2792455/147‑1 (MQ=255)
                                                                                                                                      cTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGatat           >  1:655964/1‑150 (MQ=255)
                                                                                                                                       ttCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATc          >  1:1927471/1‑150 (MQ=255)
                                                                                                                                        tCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCt         >  2:1977159/1‑150 (MQ=255)
                                                                                                                                            cGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGACTGGCAGGATATCTacac     >  1:3506649/1‑150 (MQ=255)
                                                                                                                                            cGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTacac     >  1:517857/1‑150 (MQ=255)
                                                                                                                                              aCAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACAccc   <  1:596719/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCA  >  CP009273/1478824‑1479116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTTG  >  CP009273/1478826‑1479120
                                                                                                                                                 |                                                                                                                                                     
GCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTC                                                                                                                                                   >  SRR19973061.2202361/1‑150 (MQ=60)
  GTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTG                                                                                                                                                 >  SRR19973061.2466569/1‑150 (MQ=60)
    ATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAACAGTCTGAC                                                                                                                                               >  SRR19973061.760006/1‑150 (MQ=60)
      GGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTT                                                                                                                                             >  SRR19973061.2848456/1‑150 (MQ=60)
       GTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTT                                                                                                                                            <  SRR19973061.2339792/150‑1 (MQ=60)
       GTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTT                                                                                                                                            <  SRR19973061.2339787/150‑1 (MQ=60)
       GTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTT                                                                                                                                            <  SRR19973061.129622/150‑1 (MQ=60)
        TGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTC                                                                                                                                           <  SRR19973061.291035/150‑1 (MQ=60)
          CTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTC                                                                                                                                         <  SRR19973061.2242076/150‑1 (MQ=60)
          CTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTC                                                                                                                                         <  SRR19973061.2244033/150‑1 (MQ=60)
               AGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTT                                                                                                                                    >  SRR19973061.2885382/1‑150 (MQ=60)
               AGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTT                                                                                                                                    <  SRR19973061.75813/150‑1 (MQ=60)
               AGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTT                                                                                                                                    >  SRR19973061.2885351/1‑150 (MQ=60)
                GCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTT                                                                                                                                   <  SRR19973061.3584678/150‑1 (MQ=60)
                    AAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACTTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGA                                                                                                                               >  SRR19973061.3183143/1‑150 (MQ=60)
                    AAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGA                                                                                                                               >  SRR19973061.3029819/1‑150 (MQ=60)
                      AAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAAT                                                                                                                             <  SRR19973061.2197202/150‑1 (MQ=60)
                        ACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCT                                                                                                                           <  SRR19973061.552165/150‑1 (MQ=60)
                        ACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCT                                                                                                                           <  SRR19973061.2931461/150‑1 (MQ=60)
                        ACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCT                                                                                                                           <  SRR19973061.552163/150‑1 (MQ=60)
                             GCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGA                                                                                                                      <  SRR19973061.1089157/150‑1 (MQ=60)
                             GCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGA                                                                                                                      >  SRR19973061.1803606/1‑150 (MQ=60)
                             GCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGA                                                                                                                      >  SRR19973061.1803654/1‑150 (MQ=60)
                             GCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGA                                                                                                                      >  SRR19973061.3392125/1‑150 (MQ=60)
                             GCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGA                                                                                                                      <  SRR19973061.497020/150‑1 (MQ=60)
                              CTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAA                                                                                                                     <  SRR19973061.1915959/150‑1 (MQ=60)
                              CTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAA                                                                                                                     <  SRR19973061.3731433/150‑1 (MQ=60)
                                 CGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTA                                                                                                                  <  SRR19973061.2198012/150‑1 (MQ=60)
                                  GTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAT                                                                                                                 <  SRR19973061.2667327/150‑1 (MQ=60)
                                  GTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAT                                                                                                                 <  SRR19973061.2665910/150‑1 (MQ=60)
                                  GTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAT                                                                                                                 >  SRR19973061.611621/1‑150 (MQ=60)
                                  GTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGAGCGAACGTCCGATGGACAAACAGCAGGCATCGGACAAAAAACATCGACGCTTCCACGACAAAATTTATTACATTCTCGCGTTTGTTAATCTGTGGAATTAT                                                                                                                 >  SRR19973061.1975035/1‑150 (MQ=60)
                                  GTGCGTGAGGCGATGATCATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAT                                                                                                                 <  SRR19973061.2408240/150‑1 (MQ=60)
                                   TGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATC                                                                                                                <  SRR19973061.372602/150‑1 (MQ=60)
                                   TGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATC                                                                                                                <  SRR19973061.372911/150‑1 (MQ=60)
                                    GCGTGAGGCGATGTTTATCATGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCTGATGGACAAACATCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCT                                                                                                               <  SRR19973061.722006/150‑1 (MQ=60)
                                     CGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTT                                                                                                              >  SRR19973061.3321609/1‑150 (MQ=60)
                                     CGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTT                                                                                                              >  SRR19973061.1257175/1‑150 (MQ=60)
                                        GAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATATTGGC                                                                                                           >  SRR19973061.2887484/1‑150 (MQ=60)
                                          GGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGA                                                                                                         <  SRR19973061.3092391/150‑1 (MQ=60)
                                          GGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGA                                                                                                         <  SRR19973061.2423128/150‑1 (MQ=60)
                                           GCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAG                                                                                                        >  SRR19973061.2470683/1‑150 (MQ=60)
                                             GATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCA                                                                                                      >  SRR19973061.2919393/1‑150 (MQ=60)
                                             GATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCA                                                                                                      >  SRR19973061.2469092/1‑150 (MQ=60)
                                              ATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAG                                                                                                     >  SRR19973061.3715850/1‑150 (MQ=60)
                                              ATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAG                                                                                                     <  SRR19973061.70255/150‑1 (MQ=60)
                                                 ATTATCACGTCCGCGCTCTCCTTTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAA                                                                                                  <  SRR19973061.1310744/150‑1 (MQ=60)
                                                 ATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAA                                                                                                  <  SRR19973061.2530738/150‑1 (MQ=60)
                                                 ATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAA                                                                                                  >  SRR19973061.2001797/1‑150 (MQ=60)
                                                 ATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAA                                                                                                  <  SRR19973061.2425511/150‑1 (MQ=60)
                                                 ATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAA                                                                                                  <  SRR19973061.225973/150‑1 (MQ=60)
                                                 ATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAA                                                                                                  <  SRR19973061.3304345/150‑1 (MQ=60)
                                                 ATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAA                                                                                                  >  SRR19973061.2001605/1‑150 (MQ=60)
                                                  TTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAA                                                                                                 >  SRR19973061.1789046/1‑150 (MQ=60)
                                                     TCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGG                                                                                              <  SRR19973061.1376191/150‑1 (MQ=60)
                                                      CACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGC                                                                                             <  SRR19973061.1018356/150‑1 (MQ=60)
                                                       ACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCG                                                                                            >  SRR19973061.3013221/1‑150 (MQ=60)
                                                       ACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCG                                                                                            >  SRR19973061.3013162/1‑150 (MQ=60)
                                                        CGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGC                                                                                           >  SRR19973061.1309198/1‑150 (MQ=60)
                                                           CCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTT                                                                                        <  SRR19973061.525402/150‑1 (MQ=60)
                                                              CGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTT                                                                                     >  SRR19973061.3033020/1‑150 (MQ=60)
                                                              CGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTT                                                                                     >  SRR19973061.2356772/1‑150 (MQ=60)
                                                                CTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCC                                                                                   >  SRR19973061.2637061/1‑150 (MQ=60)
                                                                   TCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAAC                                                                                <  SRR19973061.2241547/150‑1 (MQ=60)
                                                                      ATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCC                                                                             >  SRR19973061.2711965/1‑150 (MQ=60)
                                                                            GATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGT                                                                       >  SRR19973061.951523/1‑150 (MQ=60)
                                                                                  CGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTG                                                                 <  SRR19973061.3709427/150‑1 (MQ=60)
                                                                                   GCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGT                                                                <  SRR19973061.1653288/150‑1 (MQ=60)
                                                                                        CGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGT                                                           >  SRR19973061.2093680/1‑150 (MQ=60)
                                                                                         GTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTA                                                          >  SRR19973061.323569/1‑150 (MQ=60)
                                                                                         GTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTA                                                          >  SRR19973061.1982134/1‑150 (MQ=60)
                                                                                         GTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTA                                                          <  SRR19973061.1176490/150‑1 (MQ=60)
                                                                                          TCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTAC                                                         >  SRR19973061.358192/1‑150 (MQ=60)
                                                                                             GATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCCGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGA                                                      >  SRR19973061.2988448/1‑150 (MQ=60)
                                                                                              ATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGAT                                                     >  SRR19973061.3204631/1‑150 (MQ=60)
                                                                                               TGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATT                                                    <  SRR19973061.641451/150‑1 (MQ=60)
                                                                                                GGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTA                                                   >  SRR19973061.1959189/1‑150 (MQ=60)
                                                                                                   CAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCT                                                <  SRR19973061.13166/150‑1 (MQ=60)
                                                                                                   CAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATATTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCT                                                >  SRR19973061.3306072/1‑150 (MQ=60)
                                                                                                    AAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTC                                               >  SRR19973061.863998/1‑150 (MQ=60)
                                                                                                       CAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAAC                                            >  SRR19973061.184283/1‑150 (MQ=60)
                                                                                                        AGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACT                                           <  SRR19973061.2622194/150‑1 (MQ=60)
                                                                                                           AGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATC                                        >  SRR19973061.1012575/1‑150 (MQ=60)
                                                                                                            GGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCT                                       <  SRR19973061.1424561/150‑1 (MQ=60)
                                                                                                            GGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCT                                       >  SRR19973061.59257/1‑150 (MQ=60)
                                                                                                            GGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCT                                       <  SRR19973061.3327802/150‑1 (MQ=60)
                                                                                                            GGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCT                                       <  SRR19973061.3327749/150‑1 (MQ=60)
                                                                                                            GGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCT                                       <  SRR19973061.2064528/150‑1 (MQ=60)
                                                                                                             GCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTG                                      <  SRR19973061.3806310/150‑1 (MQ=60)
                                                                                                             GCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTG                                      >  SRR19973061.1063651/1‑150 (MQ=60)
                                                                                                             GCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTG                                      >  SRR19973061.1065312/1‑150 (MQ=60)
                                                                                                              CATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGC                                     >  SRR19973061.3380059/1‑150 (MQ=60)
                                                                                                              CATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGC                                     >  SRR19973061.1477777/1‑150 (MQ=60)
                                                                                                               ATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCG                                    >  SRR19973061.2559174/1‑150 (MQ=60)
                                                                                                                 CGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCG                                  <  SRR19973061.2875543/150‑1 (MQ=60)
                                                                                                                   GACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTG                                <  SRR19973061.1723735/150‑1 (MQ=60)
                                                                                                                       AAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCG                            >  SRR19973061.1418604/1‑150 (MQ=60)
                                                                                                                          AACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAAT                         <  SRR19973061.1821748/150‑1 (MQ=60)
                                                                                                                          AACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAAT                         <  SRR19973061.636824/150‑1 (MQ=60)
                                                                                                                           ACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATG                        <  SRR19973061.3246888/150‑1 (MQ=60)
                                                                                                                             ATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGC                      <  SRR19973061.758426/150‑1 (MQ=60)
                                                                                                                              TCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCA                     <  SRR19973061.350287/150‑1 (MQ=60)
                                                                                                                                GTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGG                   <  SRR19973061.2626347/150‑1 (MQ=60)
                                                                                                                                GTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGG                   >  SRR19973061.2969747/1‑150 (MQ=60)
                                                                                                                                   GCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGAGA                >  SRR19973061.2799628/1‑150 (MQ=60)
                                                                                                                                    CTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATAT               >  SRR19973061.657691/1‑150 (MQ=60)
                                                                                                                                    CTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATAT               >  SRR19973061.657650/1‑150 (MQ=60)
                                                                                                                                    CTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATAT               >  SRR19973061.886533/1‑150 (MQ=60)
                                                                                                                                     TTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATC              >  SRR19973061.16421/1‑150 (MQ=60)
                                                                                                                                     TTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATC              >  SRR19973061.1932254/1‑150 (MQ=60)
                                                                                                                                      TCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCT             <  SRR19973061.417285/150‑1 (MQ=60)
                                                                                                                                        CACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTAC           >  SRR19973061.3798841/1‑150 (MQ=60)
                                                                                                                                          CGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGACTGGCAGGATATCTACAC         >  SRR19973061.3515626/1‑150 (MQ=60)
                                                                                                                                          CGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACAC         >  SRR19973061.519220/1‑150 (MQ=60)
                                                                                                                                           GACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACC        >  SRR19973061.2808925/1‑150 (MQ=60)
                                                                                                                                            ACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCC       >  SRR19973061.2655567/1‑150 (MQ=60)
                                                                                                                                            ACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCC       <  SRR19973061.598273/150‑1 (MQ=60)
                                                                                                                                             CAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCA      >  SRR19973061.852628/1‑150 (MQ=60)
                                                                                                                                             CAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCA      >  SRR19973061.2319923/1‑150 (MQ=60)
                                                                                                                                                AAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTT   >  SRR19973061.3059198/1‑150 (MQ=60)
                                                                                                                                                 AAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTTG  <  SRR19973061.3502989/150‑1 (MQ=60)
                                                                                                                                                 AAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTTG  >  SRR19973061.857409/1‑150 (MQ=60)
                                                                                                                                                 AAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTTG  >  SRR19973061.857684/1‑150 (MQ=60)
                                                                                                                                                 AAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTTG  >  SRR19973061.2538713/1‑150 (MQ=60)
                                                                                                                                                 AAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTTG  >  SRR19973061.2538045/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
GCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAGTTG  >  CP009273/1478826‑1479120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑