Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,537,806 A→T 100% L171* (TTA→TAA)  narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,537,8060AT95.1% 569.9 / 15.1 182L171* (TTA→TAA) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base A (5/4);  new base T (97/76);  total (102/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 182 total aligned reads displayed.

CGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTT  >  CP009273/1537661‑1537952
                                                                                                                                                 |                                                                                                                                                  
cGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCACATACAGGTACAAAAATGACCAGCGGT‑CAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATc                                                                                                                                                 >  2:2651641/1‑148 (MQ=255)
cGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTAt                                                                                                                                                  >  2:2423868/1‑148 (MQ=255)
    aCCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGc                                                                                                                                              <  2:847820/148‑1 (MQ=255)
    aCCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGc                                                                                                                                              <  1:1958870/148‑1 (MQ=255)
    aCCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGc                                                                                                                                              <  1:1957087/148‑1 (MQ=255)
        aaaTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCCGCGGTGCACCCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCAtt                                                                                                                                          >  2:119442/1‑148 (MQ=255)
          aTCGATGCGTCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACACAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTTAGTTTCCTTATCCGCCATTaa                                                                                                                                        <  2:3141278/148‑1 (MQ=255)
          aTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTaa                                                                                                                                        >  1:2803518/1‑148 (MQ=255)
          aTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTaa                                                                                                                                        <  2:3141319/148‑1 (MQ=255)
           tCCATGCGGCATTCGCCAGCGACATCACCGAACCTTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAAt                                                                                                                                       <  2:2822545/148‑1 (MQ=255)
           tCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAAt                                                                                                                                       >  1:1012572/1‑148 (MQ=255)
               tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCa                                                                                                                                   <  2:3478224/148‑1 (MQ=255)
                   gCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAagag                                                                                                                               >  2:645278/1‑148 (MQ=255)
                    cATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAgc                                                                                                                              >  2:240471/1‑148 (MQ=255)
                     aTTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAgcg                                                                                                                             <  2:2050264/148‑1 (MQ=255)
                       tCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCt                                                                                                                           >  1:670991/1‑148 (MQ=255)
                       tCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCt                                                                                                                           >  1:2721693/1‑148 (MQ=255)
                          ccAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTccc                                                                                                                        <  2:1800747/148‑1 (MQ=255)
                          ccAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTccc                                                                                                                        <  2:1013170/148‑1 (MQ=255)
                           cAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCt                                                                                                                       <  2:43823/148‑1 (MQ=255)
                             gCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTg                                                                                                                     <  1:3471694/148‑1 (MQ=255)
                                aCATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGttt                                                                                                                  <  1:1860725/148‑1 (MQ=255)
                                 cATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTg                                                                                                                 >  2:1836312/1‑148 (MQ=255)
                                    cACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCt                                                                                                              <  1:1281258/148‑1 (MQ=255)
                                    cACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCt                                                                                                              <  2:2566730/148‑1 (MQ=255)
                                      ccGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCttt                                                                                                            >  2:2977681/1‑148 (MQ=255)
                                        gAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTg                                                                                                          >  1:1566506/1‑148 (MQ=255)
                                           ccGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaa                                                                                                       <  1:3098851/148‑1 (MQ=255)
                                           ccGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaa                                                                                                       <  1:3084121/148‑1 (MQ=255)
                                           ccGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaa                                                                                                       <  1:2976325/148‑1 (MQ=255)
                                            cGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaaa                                                                                                      >  2:645545/1‑148 (MQ=255)
                                                  ccTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAgaagaa                                                                                                <  1:3400833/148‑1 (MQ=255)
                                                   cTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGaaa                                                                                               <  1:2548035/148‑1 (MQ=255)
                                                   cTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGaaa                                                                                               <  1:160929/148‑1 (MQ=255)
                                                   cTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGaaa                                                                                               <  2:2217878/148‑1 (MQ=255)
                                                    tGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAAc                                                                                              <  1:2206194/148‑1 (MQ=255)
                                                     gCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACt                                                                                             >  1:3130672/1‑148 (MQ=255)
                                                      cGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTg                                                                                            <  1:1597485/148‑1 (MQ=255)
                                                      cGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTg                                                                                            <  1:120710/148‑1 (MQ=255)
                                                        gTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGat                                                                                          <  2:2254125/148‑1 (MQ=255)
                                                          aCGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGatat                                                                                        <  1:1710579/148‑1 (MQ=255)
                                                            gCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTg                                                                                      >  1:112705/1‑148 (MQ=255)
                                                            gCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTg                                                                                      >  2:1738307/1‑148 (MQ=255)
                                                                          gaAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGc                                                                        >  1:927484/1‑148 (MQ=255)
                                                                           aaaGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCa                                                                       >  2:1704269/1‑148 (MQ=255)
                                                                               gCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGt                                                                   <  2:3395024/148‑1 (MQ=255)
                                                                                    tACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCa                                                              >  1:3446083/1‑148 (MQ=255)
                                                                                      cAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAaaga                                                                                                    <  2:2932561/108‑1 (MQ=255)
                                                                                          tACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCa                                                        >  2:1119992/1‑148 (MQ=255)
                                                                                            cAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCaaa                                                      <  2:3544424/148‑1 (MQ=255)
                                                                                             aaaaaTGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCaaaa                                                     <  1:2611987/148‑1 (MQ=255)
                                                                                               aaaTGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAAcc                                                   >  1:991584/1‑148 (MQ=255)
                                                                                                aaTGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCg                                                  >  1:3497126/1‑148 (MQ=255)
                                                                                                   gACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCAt                                               <  1:761067/148‑1 (MQ=255)
                                                                                                   gACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCGTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCAt                                               <  2:3134722/148‑1 (MQ=255)
                                                                                                     ccAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAg                                             >  2:1269438/1‑148 (MQ=255)
                                                                                                     ccAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAg                                             >  2:1992487/1‑148 (MQ=255)
                                                                                                      cAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGc                                            >  2:2695409/1‑148 (MQ=255)
                                                                                                      cAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGc                                            >  1:2736149/1‑148 (MQ=255)
                                                                                                      cAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGc                                            >  1:841114/1‑148 (MQ=255)
                                                                                                        gCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaa                                          <  2:3100639/148‑1 (MQ=255)
                                                                                                         cGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                         >  2:2660670/1‑148 (MQ=255)
                                                                                                         cGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                         >  1:1143551/1‑148 (MQ=255)
                                                                                                         cGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                         >  1:1143510/1‑148 (MQ=255)
                                                                                                          ggTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAg                                        <  2:407059/148‑1 (MQ=255)
                                                                                                          ggTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAg                                        <  2:1955349/148‑1 (MQ=255)
                                                                                                           gTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGc                                       >  2:2890632/1‑148 (MQ=255)
                                                                                                           gTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACATGCAAAACCGCATAGCAAAGc                                       >  2:2520029/1‑148 (MQ=255)
                                                                                                            tGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcg                                      >  1:2997901/1‑148 (MQ=255)
                                                                                                            gtcAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcg                                      <  1:2651641/146‑1 (MQ=255)
                                                                                                              cAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgat                                    <  1:2319241/148‑1 (MQ=255)
                                                                                                                 ccAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataac                                 >  1:2076391/1‑148 (MQ=255)
                                                                                                                 ccAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataac                                 >  2:1433524/1‑148 (MQ=255)
                                                                                                                  cAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataacg                                <  1:1509912/148‑1 (MQ=255)
                                                                                                                  cAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataacg                                >  2:3521345/1‑148 (MQ=255)
                                                                                                                    gCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataacgat                              <  1:3277106/148‑1 (MQ=255)
                                                                                                                       gCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATaaa                           >  1:1009675/1‑148 (MQ=255)
                                                                                                                        cATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAat                          <  1:242367/148‑1 (MQ=255)
                                                                                                                        cATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAat                          >  1:636284/1‑148 (MQ=255)
                                                                                                                         aTTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAata                         >  1:2545521/1‑148 (MQ=255)
                                                                                                                         aTTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAata                         >  2:1664749/1‑148 (MQ=255)
                                                                                                                           tacacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATc                       >  2:509728/1‑148 (MQ=255)
                                                                                                                           tacacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATc                       >  1:63306/1‑148 (MQ=255)
                                                                                                                             cacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATccc                     >  2:907500/1‑148 (MQ=255)
                                                                                                                                 tacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCaaaa                 >  2:2608292/1‑148 (MQ=255)
                                                                                                                                 tacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCaaaa                 >  1:1465755/1‑148 (MQ=255)
                                                                                                                                   cacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAACACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAgg               >  2:2282100/1‑148 (MQ=255)
                                                                                                                                      cTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGt            <  1:2085836/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  2:927484/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  2:2724694/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  2:1240406/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  1:1822057/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  1:1987147/148‑1 (MQ=255)
                                                                                                                                        aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt          >  1:2006849/1‑148 (MQ=255)
                                                                                                                                        aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt          >  2:839956/1‑148 (MQ=255)
                                                                                                                                        aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt          <  2:1009675/148‑1 (MQ=255)
                                                                                                                                        aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt          >  1:48217/1‑148 (MQ=255)
                                                                                                                                         aGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAtt         >  1:3262022/1‑148 (MQ=255)
                                                                                                                                           tttCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCg       >  1:1273429/1‑148 (MQ=255)
                                                                                                                                              ccTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGAt    >  2:3279903/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTT  >  CP009273/1537661‑1537952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGC  >  CP009273/1537659‑1537955
                                                                                                                                                   |                                                                                                                                                     
AGCGGTACCCAAATCCCGGCCGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTAT                                                                                                                                                     <  SRR19973066.161242/150‑1 (MQ=60)
AGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTAT                                                                                                                                                     >  SRR19973066.410258/1‑150 (MQ=60)
      ACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCA                                                                                                                                               <  SRR19973066.1972724/150‑1 (MQ=60)
      ACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCA                                                                                                                                               <  SRR19973066.1970932/150‑1 (MQ=60)
        CCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATT                                                                                                                                             >  SRR19973066.1192943/1‑150 (MQ=60)
          AAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAA                                                                                                                                           >  SRR19973066.2822385/1‑150 (MQ=60)
           AATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAAT                                                                                                                                          >  SRR19973066.1020029/1‑150 (MQ=60)
                   CGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAG                                                                                                                                  <  SRR19973066.322708/150‑1 (MQ=60)
                       ATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCACGAGCGCT                                                                                                                              >  SRR19973066.675896/1‑150 (MQ=60)
                       ATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCT                                                                                                                              >  SRR19973066.2740001/1‑150 (MQ=60)
                       ATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCT                                                                                                                              >  SRR19973066.2740179/1‑150 (MQ=60)
                            CCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTT                                                                                                                         <  SRR19973066.3293894/150‑1 (MQ=60)
                               GCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTT                                                                                                                      <  SRR19973066.3494882/150‑1 (MQ=60)
                                  ACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGG                                                                                                                   <  SRR19973066.1874030/150‑1 (MQ=60)
                                      CACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTT                                                                                                               <  SRR19973066.1290517/150‑1 (MQ=60)
                                        CCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTG                                                                                                             >  SRR19973066.1577791/1‑150 (MQ=60)
                                             CCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAG                                                                                                        <  SRR19973066.2995994/150‑1 (MQ=60)
                                             CCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAG                                                                                                        <  SRR19973066.3104726/150‑1 (MQ=60)
                                             CCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAG                                                                                                        <  SRR19973066.3119546/150‑1 (MQ=60)
                                             CCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAG                                                                                                        <  SRR19973066.3119550/150‑1 (MQ=60)
                                                   GCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAA                                                                                                  <  SRR19973066.607305/150‑1 (MQ=60)
                                                    CCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAAC                                                                                                 <  SRR19973066.3423538/150‑1 (MQ=60)
                                                     CTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACT                                                                                                >  SRR19973066.3151570/1‑150 (MQ=60)
                                                     CTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACT                                                                                                <  SRR19973066.2565345/150‑1 (MQ=60)
                                                     CTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACT                                                                                                <  SRR19973066.162400/150‑1 (MQ=60)
                                                     CTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACT                                                                                                >  SRR19973066.3151132/1‑150 (MQ=60)
                                                      TGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTG                                                                                               <  SRR19973066.2221480/150‑1 (MQ=60)
                                                        CGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGAT                                                                                             <  SRR19973066.1609059/150‑1 (MQ=60)
                                                        CGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGAT                                                                                             <  SRR19973066.1608909/150‑1 (MQ=60)
                                                        CGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGAT                                                                                             <  SRR19973066.121932/150‑1 (MQ=60)
                                                            ACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTG                                                                                         <  SRR19973066.1722992/150‑1 (MQ=60)
                                                            ACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTG                                                                                         >  SRR19973066.113856/1‑150 (MQ=60)
                                                            ACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTG                                                                                         >  SRR19973066.113841/1‑150 (MQ=60)
                                                                CATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCA                                                                                     >  SRR19973066.3496130/1‑150 (MQ=60)
                                                                          GAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCCAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGC                                                                           >  SRR19973066.934197/1‑150 (MQ=60)
                                                                                    AATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCA                                                                 >  SRR19973066.3469133/1‑150 (MQ=60)
                                                                                      TNCAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAgatcggaagagcacacgtctgaactccagtcactacc                                                               >  SRR19973066.2952080/1‑113 (MQ=60)
                                                                                        CAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTG                                                             >  SRR19973066.975367/1‑150 (MQ=60)
                                                                                               AAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACC                                                      <  SRR19973066.2629641/150‑1 (MQ=60)
                                                                                               AAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACC                                                      >  SRR19973066.998892/1‑150 (MQ=60)
                                                                                                AAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCG                                                     >  SRR19973066.3520440/1‑150 (MQ=60)
                                                                                                 AAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGC                                                    >  SRR19973066.2523174/1‑150 (MQ=60)
                                                                                                   ATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCAT                                                  >  SRR19973066.653070/1‑150 (MQ=60)
                                                                                                     GACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAG                                                <  SRR19973066.766709/150‑1 (MQ=60)
                                                                                                     GACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAG                                                <  SRR19973066.767453/150‑1 (MQ=60)
                                                                                                      ACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGC                                               >  SRR19973066.2754550/1‑150 (MQ=60)
                                                                                                      ACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGC                                               >  SRR19973066.2427351/1‑150 (MQ=60)
                                                                                                      ACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGC                                               >  SRR19973066.847226/1‑150 (MQ=60)
                                                                                                       CCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCA                                              >  SRR19973066.156255/1‑150 (MQ=60)
                                                                                                         AGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAA                                            >  SRR19973066.1151678/1‑150 (MQ=60)
                                                                                                         AGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAA                                            >  SRR19973066.1151720/1‑150 (MQ=60)
                                                                                                         AGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAA                                            >  SRR19973066.1151690/1‑150 (MQ=60)
                                                                                                            GGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCG                                         >  SRR19973066.3017796/1‑150 (MQ=60)
                                                                                                              gtCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGAT                                       <  SRR19973066.2669441/148‑1 (MQ=60)
                                                                                                                CAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAA                                     <  SRR19973066.2335094/150‑1 (MQ=60)
                                                                                                                 AACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAAC                                    >  SRR19973066.2090742/1‑150 (MQ=60)
                                                                                                                 AACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAAC                                    >  SRR19973066.3481053/1‑150 (MQ=60)
                                                                                                                 AACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAAC                                    >  SRR19973066.2091258/1‑150 (MQ=60)
                                                                                                                    CAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGAT                                 <  SRR19973066.1520475/150‑1 (MQ=60)
                                                                                                                     AGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATA                                <  SRR19973066.3464643/150‑1 (MQ=60)
                                                                                                                      GCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAA                               <  SRR19973066.3298623/150‑1 (MQ=60)
                                                                                                                       CTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAA                              >  SRR19973066.1665168/1‑150 (MQ=60)
                                                                                                                       CTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAA                              >  SRR19973066.1017114/1‑150 (MQ=60)
                                                                                                                        TGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAAT                             >  SRR19973066.640869/1‑150 (MQ=60)
                                                                                                                         GCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATA                            >  SRR19973066.2562882/1‑150 (MQ=60)
                                                                                                                         GCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATA                            >  SRR19973066.2562817/1‑150 (MQ=60)
                                                                                                                          CATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATAT                           <  SRR19973066.244261/150‑1 (MQ=60)
                                                                                                                           ATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATC                          >  SRR19973066.1984934/1‑150 (MQ=60)
                                                                                                                           ATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATC                          >  SRR19973066.64084/1‑150 (MQ=60)
                                                                                                                             TACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCC                        <  SRR19973066.739565/150‑1 (MQ=60)
                                                                                                                             TACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCC                        >  SRR19973066.1504987/1‑150 (MQ=60)
                                                                                                                                 CTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAA                    >  SRR19973066.1476098/1‑150 (MQ=60)
                                                                                                                                 CTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAA                    >  SRR19973066.1476100/1‑150 (MQ=60)
                                                                                                                                  TTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAG                   >  SRR19973066.1736439/1‑150 (MQ=60)
                                                                                                                                   TACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGG                  <  SRR19973066.2580146/150‑1 (MQ=60)
                                                                                                                                    ACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGA                 >  SRR19973066.1143909/1‑150 (MQ=60)
                                                                                                                                      ACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGT               >  SRR19973066.3012408/1‑150 (MQ=60)
                                                                                                                                        CTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAT             >  SRR19973066.2066162/1‑150 (MQ=60)
                                                                                                                                        CTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAT             <  SRR19973066.2100222/150‑1 (MQ=60)
                                                                                                                                        CTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAT             >  SRR19973066.2020930/1‑150 (MQ=60)
                                                                                                                                        CTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAT             >  SRR19973066.2020780/1‑150 (MQ=60)
                                                                                                                                        CTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAT             <  SRR19973066.1925480/150‑1 (MQ=60)
                                                                                                                                        CTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAT             >  SRR19973066.48834/1‑150 (MQ=60)
                                                                                                                                         TAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATT            >  SRR19973066.3283471/1‑150 (MQ=60)
                                                                                                                                         TAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATT            <  SRR19973066.2001154/150‑1 (MQ=60)
                                                                                                                                         TAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATT            <  SRR19973066.1835048/150‑1 (MQ=60)
                                                                                                                                           AGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCG          >  SRR19973066.1282622/1‑150 (MQ=60)
                                                                                                                                           AGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCG          >  SRR19973066.1283766/1‑150 (MQ=60)
                                                                                                                                             TTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGA        >  SRR19973066.2795661/1‑150 (MQ=60)
                                                                                                                                             TTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGA        >  SRR19973066.1864454/1‑150 (MQ=60)
                                                                                                                                             TTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGA        >  SRR19973066.889885/1‑150 (MQ=60)
                                                                                                                                              TTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGAT       >  SRR19973066.2202398/1‑150 (MQ=60)
                                                                                                                                                CCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTT     <  SRR19973066.2713423/150‑1 (MQ=60)
                                                                                                                                                 CTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTT    >  SRR19973066.1552162/1‑150 (MQ=60)
                                                                                                                                                 CTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTT    >  SRR19973066.3088319/1‑150 (MQ=60)
                                                                                                                                                 CTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTT    >  SRR19973066.3088724/1‑150 (MQ=60)
                                                                                                                                                   TATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGC  >  SRR19973066.3002408/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGC  >  CP009273/1537659‑1537955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑