Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,548,216 A→T 100% intergenic (‑121/+13) adhP ← / ← maeA ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,548,2160AT99.1% 740.7 / ‑2.7 225intergenic (‑121/+13)adhP/maeAethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (119/104);  total (121/104)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

CTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC  >  CP009273/1548072‑1548360
                                                                                                                                                |                                                                                                                                                
cTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCg                                                                                                                                               <  2:923273/148‑1 (MQ=255)
  tCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCggg                                                                                                                                             >  2:3105353/1‑148 (MQ=255)
  tCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCggg                                                                                                                                             <  2:1585741/148‑1 (MQ=255)
  tCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCggg                                                                                                                                             <  1:3277320/148‑1 (MQ=255)
     tAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGgcgc                                                                                                                                          <  1:125060/148‑1 (MQ=255)
          acTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAGAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCt                                                                                                                                     <  1:1515078/148‑1 (MQ=255)
            tGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTa                                                                                                                                   >  1:2677616/1‑148 (MQ=255)
            tGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTa                                                                                                                                   >  1:1896561/1‑148 (MQ=255)
             gCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAg                                                                                                                                  >  2:1722726/1‑148 (MQ=255)
              cAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAACAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGa                                                                                                                                 >  2:3548151/1‑148 (MQ=255)
              cAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAGGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGa                                                                                                                                 >  1:2503722/1‑148 (MQ=255)
                gCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATg                                                                                                                               >  1:3124622/1‑148 (MQ=255)
                gCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATg                                                                                                                               >  1:2554375/1‑148 (MQ=255)
                 ccTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATgg                                                                                                                              >  1:3482362/1‑148 (MQ=255)
                 ccTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATgg                                                                                                                              <  2:3128686/148‑1 (MQ=255)
                  cTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGa                                                                                                                             <  2:912269/148‑1 (MQ=255)
                   ttCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAg                                                                                                                            >  1:1911276/1‑148 (MQ=255)
                   ttCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAg                                                                                                                            <  2:1079753/148‑1 (MQ=255)
                    tCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAgg                                                                                                                           >  1:3284113/1‑148 (MQ=255)
                     cATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGt                                                                                                                          >  2:2605364/1‑148 (MQ=255)
                          ttCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggc                                                                                                                     >  2:2143309/1‑148 (MQ=255)
                             ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGt                                                                                                                  <  2:131409/148‑1 (MQ=255)
                             ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGt                                                                                                                  <  2:2682989/148‑1 (MQ=255)
                               ccgtggcGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAGTTATTCGCCACTACCCAGTATGGATGAGATCGGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTGCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAg                                                                                                                <  2:2763625/142‑1 (MQ=255)
                                  tttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                                                                                             >  2:3461971/1‑148 (MQ=255)
                                  tttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                                                                                             <  1:2309240/148‑1 (MQ=255)
                                  tttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                                                                                             <  2:2473310/148‑1 (MQ=255)
                                     cGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCgg                                                                                                          <  2:1756232/148‑1 (MQ=255)
                                      ggATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAGTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGt                                                                                                         <  1:2829233/148‑1 (MQ=255)
                                        atgatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt                                                                                                       <  1:2688573/148‑1 (MQ=255)
                                        atgatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt                                                                                                       <  1:2372370/148‑1 (MQ=255)
                                          gatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc                                                                                                     >  1:2214595/1‑148 (MQ=255)
                                           atgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCg                                                                                                    >  2:1505322/1‑148 (MQ=255)
                                           atgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCg                                                                                                    >  1:1019153/1‑148 (MQ=255)
                                            tgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCgg                                                                                                   >  2:598930/1‑148 (MQ=255)
                                             gTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGc                                                                                                  <  2:3513789/148‑1 (MQ=255)
                                             gTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGc                                                                                                  <  2:564403/148‑1 (MQ=255)
                                               tCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCtt                                                                                                >  1:3232688/1‑148 (MQ=255)
                                               tCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCtt                                                                                                >  1:342345/1‑148 (MQ=255)
                                                     tAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGa                                                                                          >  2:1145901/1‑148 (MQ=255)
                                                       gCAGGTGAGGCAAATGATATTTATTCTCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGaaa                                                                                        <  2:2696113/148‑1 (MQ=255)
                                                        cAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAt                                                                                       >  1:2234668/1‑148 (MQ=255)
                                                         aGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAtt                                                                                      <  2:349993/148‑1 (MQ=255)
                                                           gTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt                                                                                    >  1:1112038/1‑148 (MQ=255)
                                                             gAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCg                                                                                  <  2:3478672/148‑1 (MQ=255)
                                                             gAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCg                                                                                  >  1:2164007/1‑148 (MQ=255)
                                                             gAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCg                                                                                  <  1:404000/148‑1 (MQ=255)
                                                                 cAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCaa                                                                              >  1:114407/1‑148 (MQ=255)
                                                                 cAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCaa                                                                              <  2:248646/148‑1 (MQ=255)
                                                                    ctGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATgg                                                                           <  2:715745/147‑1 (MQ=255)
                                                                    aTGATATTTATTCGCCACTACCCAGTATGGAGGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATgg                                                                           >  1:3530630/1‑148 (MQ=255)
                                                                     tGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGc                                                                          <  1:1690088/148‑1 (MQ=255)
                                                                     tGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGc                                                                          <  1:1684613/148‑1 (MQ=255)
                                                                     tGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGc                                                                          >  2:322686/1‑148 (MQ=255)
                                                                        taTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTg                                                                       <  2:106680/147‑1 (MQ=255)
                                                                         aTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGt                                                                      >  1:907644/1‑148 (MQ=255)
                                                                         aTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGt                                                                      >  1:907436/1‑148 (MQ=255)
                                                                          tttATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGtt                                                                     >  1:2723218/1‑148 (MQ=255)
                                                                          tttATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGtt                                                                     >  2:2001869/1‑148 (MQ=255)
                                                                             aTTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCa                                                                  <  2:2902928/148‑1 (MQ=255)
                                                                              ttCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAg                                                                 >  2:788396/1‑148 (MQ=255)
                                                                              ttCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAg                                                                 >  1:251988/1‑148 (MQ=255)
                                                                               tCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAgg                                                                >  2:1253993/1‑148 (MQ=255)
                                                                                cGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAggg                                                               <  1:1813921/148‑1 (MQ=255)
                                                                                 gCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGc                                                              <  2:1120597/148‑1 (MQ=255)
                                                                                    aCTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTc                                                           >  2:446748/1‑148 (MQ=255)
                                                                                     cTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCTTCAATGGCCTGTTGCAGGGCTTCg                                                          >  2:1492107/1‑148 (MQ=255)
                                                                                         ccAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCag                                                      <  1:76313/148‑1 (MQ=255)
                                                                                         ccAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCag                                                      >  1:2579620/1‑148 (MQ=255)
                                                                                         ccAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCag                                                      >  1:1321209/1‑148 (MQ=255)
                                                                                         ccAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCag                                                      >  1:973113/1‑148 (MQ=255)
                                                                                          cAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCaga                                                     <  2:2234668/148‑1 (MQ=255)
                                                                                          cAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCaga                                                     >  2:3098098/1‑148 (MQ=255)
                                                                                            gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAgg                                                   >  1:3054162/1‑148 (MQ=255)
                                                                                               tGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGttt                                                <  1:1740284/148‑1 (MQ=255)
                                                                                                    gagaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCAcc                                           >  1:473450/1‑148 (MQ=255)
                                                                                                      gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGc                                         <  1:598930/148‑1 (MQ=255)
                                                                                                        tCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCa                                       >  1:807249/1‑148 (MQ=255)
                                                                                                         cTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCAc                                      >  1:1441762/1‑148 (MQ=255)
                                                                                                           gAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGc                                    <  1:2976006/148‑1 (MQ=255)
                                                                                                            aaaaaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGcc                                   <  1:1871923/148‑1 (MQ=255)
                                                                                                            aaaaaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGcc                                   <  2:166754/148‑1 (MQ=255)
                                                                                                             aaaaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCt                                  >  2:2697463/1‑148 (MQ=255)
                                                                                                             aaaaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCt                                  >  1:1500320/1‑148 (MQ=255)
                                                                                                               aaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtg                                >  1:969852/1‑148 (MQ=255)
                                                                                                               aaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtg                                >  2:3330882/1‑148 (MQ=255)
                                                                                                                      agGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtgctgctgc                         >  2:961109/1‑148 (MQ=255)
                                                                                                                        ggAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTgcgc                       <  1:2884040/148‑1 (MQ=255)
                                                                                                                        ggAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTgcgc                       <  1:2819548/148‑1 (MQ=255)
                                                                                                                         gAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGcc                      <  2:2441080/148‑1 (MQ=255)
                                                                                                                          aaaTAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCa                     <  2:2554832/148‑1 (MQ=255)
                                                                                                                                cccGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGc               <  2:2306143/148‑1 (MQ=255)
                                                                                                                                   ggTAGCCTTCACGTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCACTGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCaa            <  1:1963040/148‑1 (MQ=255)
                                                                                                                                     tAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAAcc          >  1:2868102/1‑148 (MQ=255)
                                                                                                                                       gCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGc        >  2:273648/1‑148 (MQ=255)
                                                                                                                                       gCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGc        >  2:644609/1‑148 (MQ=255)
                                                                                                                                        ccTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCa       >  2:2208931/1‑148 (MQ=255)
                                                                                                                                          ttCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCaaa     >  2:3269632/1‑148 (MQ=255)
                                                                                                                                             aCTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGc  >  1:952742/1‑148 (MQ=255)
                                                                                                                                             aCTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGc  >  1:952699/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC  >  CP009273/1548072‑1548360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAAT  >  CP009273/1548067‑1548363
                                                                                                                                                     |                                                                                                                                                   
TGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTT                                                                                                                                                     >  SRR19973066.2488452/1‑150 (MQ=60)
TGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTT                                                                                                                                                     >  SRR19973066.2490173/1‑150 (MQ=60)
    CCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGAGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGG                                                                                                                                                 >  SRR19973066.2912831/1‑150 (MQ=60)
       TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCG                                                                                                                                              <  SRR19973066.1697489/150‑1 (MQ=60)
       TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCG                                                                                                                                              <  SRR19973066.3298876/150‑1 (MQ=60)
       TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCG                                                                                                                                              <  SRR19973066.3298839/150‑1 (MQ=60)
          TAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAG                                                                                                                                           <  SRR19973066.126304/150‑1 (MQ=60)
               ACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATATCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAGAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTA                                                                                                                                      <  SRR19973066.1525718/150‑1 (MQ=60)
               ACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTA                                                                                                                                      <  SRR19973066.564674/150‑1 (MQ=60)
               ACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTA                                                                                                                                      >  SRR19973066.2695493/1‑150 (MQ=60)
               ACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTA                                                                                                                                      >  SRR19973066.1910086/1‑150 (MQ=60)
                 TGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAGGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGA                                                                                                                                    >  SRR19973066.2520774/1‑150 (MQ=60)
                   CAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATG                                                                                                                                  >  SRR19973066.3145487/1‑150 (MQ=60)
                   CAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATG                                                                                                                                  >  SRR19973066.2571722/1‑150 (MQ=60)
                   CAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATG                                                                                                                                  >  SRR19973066.2572182/1‑150 (MQ=60)
                    AGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGG                                                                                                                                 >  SRR19973066.3505597/1‑150 (MQ=60)
                    AGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGG                                                                                                                                 >  SRR19973066.3506408/1‑150 (MQ=60)
                     GCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTCGCCTTCACTTCCGGGCGCAGGCTTAGATGGA                                                                                                                                >  SRR19973066.1999630/1‑150 (MQ=60)
                      CCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAG                                                                                                                               <  SRR19973066.3044837/150‑1 (MQ=60)
                      CCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAG                                                                                                                               >  SRR19973066.1924884/1‑150 (MQ=60)
                       CTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGG                                                                                                                              <  SRR19973066.676112/150‑1 (MQ=60)
                       CTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGG                                                                                                                              >  SRR19973066.3305729/1‑150 (MQ=60)
                          CATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAC                                                                                                                           <  SRR19973066.1908347/150‑1 (MQ=60)
                           ATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACG                                                                                                                          <  SRR19973066.1581436/150‑1 (MQ=60)
                             AGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGC                                                                                                                        >  SRR19973066.2895162/1‑150 (MQ=60)
                             AGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGC                                                                                                                        >  SRR19973066.2321938/1‑150 (MQ=60)
                              GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                                                                                       >  SRR19973066.326355/1‑150 (MQ=60)
                              GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATGCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                                                                                       >  SRR19973066.326491/1‑150 (MQ=60)
                                 CCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAAGGCGGTA                                                                                                                    >  SRR19973066.923716/1‑150 (MQ=60)
                                  CTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAG                                                                                                                   >  SRR19973066.2776707/1‑150 (MQ=60)
                                  CTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAG                                                                                                                   >  SRR19973066.168261/1‑150 (MQ=60)
                                       TTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCG                                                                                                              <  SRR19973066.2325049/150‑1 (MQ=60)
                                       TTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCG                                                                                                              <  SRR19973066.442040/150‑1 (MQ=60)
                                           GGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAT                                                                                                          >  SRR19973066.1297844/1‑150 (MQ=60)
                                           GGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAT                                                                                                          <  SRR19973066.2848128/150‑1 (MQ=60)
                                           GGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAGTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAT                                                                                                          <  SRR19973066.2848241/150‑1 (MQ=60)
                                             ATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTC                                                                                                        <  SRR19973066.2706517/150‑1 (MQ=60)
                                             ATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTC                                                                                                        >  SRR19973066.2229930/1‑150 (MQ=60)
                                             ATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTC                                                                                                        >  SRR19973066.2230292/1‑150 (MQ=60)
                                             ATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTC                                                                                                        <  SRR19973066.2388401/150‑1 (MQ=60)
                                              TGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCG                                                                                                       >  SRR19973066.1026652/1‑150 (MQ=60)
                                                  GTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTT                                                                                                   >  SRR19973066.3254039/1‑150 (MQ=60)
                                                  GTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTT                                                                                                   <  SRR19973066.1233638/150‑1 (MQ=60)
                                                  GTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTT                                                                                                   >  SRR19973066.344990/1‑150 (MQ=60)
                                                          TAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAA                                                                                           >  SRR19973066.2541540/1‑150 (MQ=60)
                                                           AGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAT                                                                                          >  SRR19973066.2250087/1‑150 (MQ=60)
                                                            GCAGGTGAGGCATATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATT                                                                                         <  SRR19973066.2158187/150‑1 (MQ=60)
                                                             CAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTA                                                                                        <  SRR19973066.1968215/150‑1 (MQ=60)
                                                             CAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTA                                                                                        >  SRR19973066.1059039/1‑150 (MQ=60)
                                                              AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTTCGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                                                                                       <  SRR19973066.1763147/150‑1 (MQ=60)
                                                              AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                                                                                       >  SRR19973066.1120074/1‑150 (MQ=60)
                                                                GTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCG                                                                                     >  SRR19973066.2179040/1‑150 (MQ=60)
                                                                 TGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGT                                                                                    >  SRR19973066.1914411/1‑150 (MQ=60)
                                                                  GAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTC                                                                                   <  SRR19973066.1462322/150‑1 (MQ=60)
                                                                  GAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTC                                                                                   <  SRR19973066.407181/150‑1 (MQ=60)
                                                                    GGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAA                                                                                 >  SRR19973066.115574/1‑150 (MQ=60)
                                                                       AAATGATATTTATTCGCCACTACCCAGTATGGAGGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGG                                                                              >  SRR19973066.3554111/1‑150 (MQ=60)
                                                                        AATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGC                                                                             >  SRR19973066.1391810/1‑150 (MQ=60)
                                                                        AATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGC                                                                             >  SRR19973066.2880245/1‑150 (MQ=60)
                                                                         ATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCC                                                                            <  SRR19973066.1125282/150‑1 (MQ=60)
                                                                          TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT                                                                           <  SRR19973066.355206/150‑1 (MQ=60)
                                                                          TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT                                                                           <  SRR19973066.1702387/150‑1 (MQ=60)
                                                                          TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT                                                                           <  SRR19973066.1696882/150‑1 (MQ=60)
                                                                          TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT                                                                           <  SRR19973066.1703521/150‑1 (MQ=60)
                                                                            ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT                                                                         >  SRR19973066.914059/1‑150 (MQ=60)
                                                                            ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT                                                                         >  SRR19973066.913849/1‑150 (MQ=60)
                                                                            ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT                                                                         >  SRR19973066.913850/1‑150 (MQ=60)
                                                                             TATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT                                                                        >  SRR19973066.2741537/1‑150 (MQ=60)
                                                                                 TATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAG                                                                    >  SRR19973066.2708798/1‑150 (MQ=60)
                                                                                 TATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAG                                                                    >  SRR19973066.253914/1‑150 (MQ=60)
                                                                                 TATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAG                                                                    <  SRR19973066.2958639/150‑1 (MQ=60)
                                                                                     CGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCT                                                                <  SRR19973066.1826833/150‑1 (MQ=60)
                                                                                      GCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTT                                                               <  SRR19973066.1272776/150‑1 (MQ=60)
                                                                                       CCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTC                                                              <  SRR19973066.2809533/150‑1 (MQ=60)
                                                                                          CTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGC                                                           >  SRR19973066.2588146/1‑150 (MQ=60)
                                                                                           TACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCA                                                          >  SRR19973066.2478457/1‑150 (MQ=60)
                                                                                            ACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAG                                                         >  SRR19973066.2597113/1‑150 (MQ=60)
                                                                                            ACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAG                                                         <  SRR19973066.406439/150‑1 (MQ=60)
                                                                                            ACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAG                                                         >  SRR19973066.2597216/1‑150 (MQ=60)
                                                                                            ACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAG                                                         >  SRR19973066.980267/1‑150 (MQ=60)
                                                                                            ACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAG                                                         >  SRR19973066.1330785/1‑150 (MQ=60)
                                                                                            ACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAG                                                         <  SRR19973066.233403/150‑1 (MQ=60)
                                                                                            ACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAG                                                         >  SRR19973066.978614/1‑150 (MQ=60)
                                                                                             CCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGA                                                        <  SRR19973066.885450/150‑1 (MQ=60)
                                                                                             CCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGA                                                        >  SRR19973066.2532490/1‑150 (MQ=60)
                                                                                              CCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAG                                                       <  SRR19973066.77206/150‑1 (MQ=60)
                                                                                               CAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGG                                                      >  SRR19973066.3074589/1‑150 (MQ=60)
                                                                                                  TATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTT                                                   >  SRR19973066.2380549/1‑150 (MQ=60)
                                                                                                   ATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTT                                                  <  SRR19973066.3464756/150‑1 (MQ=60)
                                                                                                    TGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTC                                                 >  SRR19973066.151863/1‑150 (MQ=60)
                                                                                                    TGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTC                                                 <  SRR19973066.1752835/150‑1 (MQ=60)
                                                                                                     GGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCA                                                >  SRR19973066.3231178/1‑150 (MQ=60)
                                                                                                       ATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACC                                              >  SRR19973066.477033/1‑150 (MQ=60)
                                                                                                           GATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCA                                          <  SRR19973066.603059/150‑1 (MQ=60)
                                                                                                           GATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCA                                          >  SRR19973066.813177/1‑150 (MQ=60)
                                                                                                            ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCAC                                         >  SRR19973066.1452022/1‑150 (MQ=60)
                                                                                                             TCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACG                                        <  SRR19973066.983327/150‑1 (MQ=60)
                                                                                                                GAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCT                                     >  SRR19973066.1510775/1‑150 (MQ=60)
                                                                                                                GAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCT                                     <  SRR19973066.2995670/150‑1 (MQ=60)
                                                                                                                 AAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTT                                    <  SRR19973066.781427/150‑1 (MQ=60)
                                                                                                                 AAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTT                                    <  SRR19973066.1885292/150‑1 (MQ=60)
                                                                                                                  AAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTG                                   >  SRR19973066.976988/1‑150 (MQ=60)
                                                                                                                       GGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCT                              >  SRR19973066.2704928/1‑150 (MQ=60)
                                                                                                                       GGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCT                              >  SRR19973066.3384541/1‑150 (MQ=60)
                                                                                                                             GGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCA                        <  SRR19973066.2903348/150‑1 (MQ=60)
                                                                                                                             GGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCA                        <  SRR19973066.2838496/150‑1 (MQ=60)
                                                                                                                                AATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTT                     >  SRR19973066.638402/1‑150 (MQ=60)
                                                                                                                                AATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTT                     >  SRR19973066.1829101/1‑150 (MQ=60)
                                                                                                                                   AGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGC                  >  SRR19973066.3078888/1‑150 (MQ=60)
                                                                                                                                      CCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAA               >  SRR19973066.1872301/1‑150 (MQ=60)
                                                                                                                                        GGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACC             >  SRR19973066.2887340/1‑150 (MQ=60)
                                                                                                                                        GGTAGCCTTCACGTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCACTGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACC             <  SRR19973066.1976918/150‑1 (MQ=60)
                                                                                                                                            GCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAA         >  SRR19973066.2223668/1‑150 (MQ=60)
                                                                                                                                                TCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC     >  SRR19973066.959695/1‑150 (MQ=60)
                                                                                                                                                TCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC     >  SRR19973066.959700/1‑150 (MQ=60)
                                                                                                                                                TCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC     >  SRR19973066.959714/1‑150 (MQ=60)
                                                                                                                                                TCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC     >  SRR19973066.959657/1‑150 (MQ=60)
                                                                                                                                                TCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC     >  SRR19973066.959618/1‑150 (MQ=60)
                                                                                                                                                   CTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAAT  <  SRR19973066.3147806/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAAT  >  CP009273/1548067‑1548363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑