Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,548,216 A→T 100% intergenic (‑121/+13) adhP ← / ← maeA ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,548,2160AT98.0% 676.5 / 1.0 210intergenic (‑121/+13)adhP/maeAethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base T (112/93);  total (116/94)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC  >  CP009273/1548074‑1548360
                                                                                                                                              |                                                                                                                                                
tCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCggg                                                                                                                                             <  1:851181/148‑1 (MQ=255)
   tAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGgcgc                                                                                                                                          >  1:310795/1‑148 (MQ=255)
   tAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAAGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGgcgc                                                                                                                                          >  1:2553791/1‑148 (MQ=255)
    aacaacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCa                                                                                                                                         >  2:2659957/1‑148 (MQ=255)
        acTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCt                                                                                                                                     >  1:687601/1‑148 (MQ=255)
          tGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTa                                                                                                                                   <  2:2937944/148‑1 (MQ=255)
            cAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTAGCCACTACCCAGTATGGATGAGATCTGAAAATGGGAGAGGGAAATATCCAGGTAGCCGTCACTTACGGGCGCAGCCTTAGa                                                                                                                                 >  2:2076354/1‑148 (MQ=255)
               ccTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATgg                                                                                                                              >  2:2298381/1‑148 (MQ=255)
               ccTTCAAAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATgg                                                                                                                              >  2:1058185/1‑148 (MQ=255)
                 ttCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAg                                                                                                                            <  1:1827363/148‑1 (MQ=255)
                   cATAGGTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGt                                                                                                                          <  2:628908/148‑1 (MQ=255)
                      aGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcg                                                                                                                       >  1:2909833/1‑148 (MQ=255)
                         tcctccTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcg                                                                                                                    >  1:1413690/1‑148 (MQ=255)
                         tcctccTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcg                                                                                                                    >  1:1499576/1‑148 (MQ=255)
                         tcctccTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcg                                                                                                                    <  1:3291823/148‑1 (MQ=255)
                          cctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcgg                                                                                                                   <  1:2139193/148‑1 (MQ=255)
                           ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGt                                                                                                                  <  1:1928050/148‑1 (MQ=255)
                           ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGt                                                                                                                  >  1:1237203/1‑148 (MQ=255)
                           ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGt                                                                                                                  <  2:1621828/148‑1 (MQ=255)
                             cctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAg                                                                                                                <  1:628443/148‑1 (MQ=255)
                              ctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGt                                                                                                               >  2:2896195/1‑148 (MQ=255)
                                tttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                                                                                             >  1:1228442/1‑148 (MQ=255)
                                   cGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCgg                                                                                                          <  1:2192501/148‑1 (MQ=255)
                                    ggATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGt                                                                                                         <  1:1652821/148‑1 (MQ=255)
                                     gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTa                                                                                                        <  2:2134682/148‑1 (MQ=255)
                                       tgatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGGACGGCGGTAGTCGCGGTAtt                                                                                                      <  2:578742/148‑1 (MQ=255)
                                        gatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc                                                                                                     >  2:3089560/1‑148 (MQ=255)
                                        gatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc                                                                                                     >  1:1231495/1‑148 (MQ=255)
                                         atgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCg                                                                                                    >  2:1930518/1‑148 (MQ=255)
                                          tgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCgg                                                                                                   >  2:2170421/1‑148 (MQ=255)
                                          tgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCgg                                                                                                   <  1:2055855/148‑1 (MQ=255)
                                           gTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGc                                                                                                  >  2:3113180/1‑148 (MQ=255)
                                             tCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCtt                                                                                                >  2:3360201/1‑148 (MQ=255)
                                              cTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTg                                                                                               >  2:2578657/1‑148 (MQ=255)
                                               tGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGc                                                                                              <  2:863698/148‑1 (MQ=255)
                                               tGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGc                                                                                              <  2:2861511/148‑1 (MQ=255)
                                                gCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGcc                                                                                             >  1:1396958/1‑148 (MQ=255)
                                                gCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGcc                                                                                             >  2:3481763/1‑148 (MQ=255)
                                                 cATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCa                                                                                            >  2:1061401/1‑148 (MQ=255)
                                                     gCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGaaa                                                                                        >  1:1007724/1‑148 (MQ=255)
                                                     gCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGaaa                                                                                        <  1:1931434/148‑1 (MQ=255)
                                                       aGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAtt                                                                                      <  1:1315253/148‑1 (MQ=255)
                                                        ggTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTa                                                                                     <  2:566669/148‑1 (MQ=255)
                                                          tGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCTGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATc                                                                                   >  1:2649848/1‑148 (MQ=255)
                                                          tGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATc                                                                                   >  2:2552832/1‑148 (MQ=255)
                                                          tGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATc                                                                                   <  1:776017/148‑1 (MQ=255)
                                                           gAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCg                                                                                  <  2:2975037/148‑1 (MQ=255)
                                                           gAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCg                                                                                  <  2:1348358/148‑1 (MQ=255)
                                                               cAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCaa                                                                              <  2:1305193/148‑1 (MQ=255)
                                                                aaaTGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAAt                                                                             >  2:1544629/1‑148 (MQ=255)
                                                                aaaTGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAAt                                                                             <  2:1129011/148‑1 (MQ=255)
                                                                  aTGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATgg                                                                           >  2:2967309/1‑148 (MQ=255)
                                                                   tGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGc                                                                          <  1:97604/148‑1 (MQ=255)
                                                                     ataTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCt                                                                        >  1:268795/3‑148 (MQ=255)
                                                                     ataTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCt                                                                        >  1:2215476/3‑148 (MQ=255)
                                                                      taTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTg                                                                       >  2:1811197/2‑148 (MQ=255)
                                                                         ttATTCGCCACTACCCAGTAAGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCCGAACTTATCGTCAATGGCCTGGTg                                                                    >  2:2424035/1‑148 (MQ=255)
                                                                           aTTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCa                                                                  >  1:822961/1‑148 (MQ=255)
                                                                             tCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAgg                                                                >  2:2344933/1‑148 (MQ=255)
                                                                               gCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGc                                                              <  2:146361/148‑1 (MQ=255)
                                                                                ccACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCt                                                             >  1:253801/1‑148 (MQ=255)
                                                                                ccACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCt                                                             <  1:547644/148‑1 (MQ=255)
                                                                                 cACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCtt                                                            >  2:1259901/1‑148 (MQ=255)
                                                                                    tACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCgg                                                         <  1:2531487/148‑1 (MQ=255)
                                                                                       ccAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCag                                                      >  2:27189/1‑148 (MQ=255)
                                                                                        cAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCaga                                                     >  1:395449/1‑148 (MQ=255)
                                                                                          gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAgg                                                   <  1:2085142/148‑1 (MQ=255)
                                                                                            aTGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGtt                                                 <  2:687601/148‑1 (MQ=255)
                                                                                               gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTc                                              >  2:1588742/1‑148 (MQ=255)
                                                                                                aagagaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCa                                             <  1:232459/146‑1 (MQ=255)
                                                                                                aTGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCa                                             <  1:2479284/148‑1 (MQ=255)
                                                                                                  gagaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCAcc                                           >  2:1074824/1‑148 (MQ=255)
                                                                                                      tCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCa                                       <  2:1934269/148‑1 (MQ=255)
                                                                                                      acTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCa                                       <  2:1194218/147‑1 (MQ=255)
                                                                                                           aaaaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCt                                  >  2:465878/1‑148 (MQ=255)
                                                                                                              aGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtgc                               >  1:146043/1‑148 (MQ=255)
                                                                                                                ggAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtgctg                             >  2:2029861/1‑148 (MQ=255)
                                                                                                                ggAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtgctg                             >  1:2942454/1‑148 (MQ=255)
                                                                                                                ggAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtgctg                             >  1:379308/1‑148 (MQ=255)
                                                                                                                     gggAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTgcg                        >  1:2801513/1‑148 (MQ=255)
                                                                                                                      ggAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTgcgc                       >  1:1829199/1‑148 (MQ=255)
                                                                                                                      ggAAATAGCCCGGTAGCCTTCACGTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTgcgc                       <  2:2400113/148‑1 (MQ=255)
                                                                                                                           tAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCAttt                  <  2:1390457/148‑1 (MQ=255)
                                                                                                                           tAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCAttt                  <  1:1808848/148‑1 (MQ=255)
                                                                                                                           tAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCAttt                  >  1:843142/1‑148 (MQ=255)
                                                                                                                             gCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTg                <  2:915168/148‑1 (MQ=255)
                                                                                                                              cccGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGc               >  1:621188/1‑148 (MQ=255)
                                                                                                                              cccGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGc               >  1:595894/1‑148 (MQ=255)
                                                                                                                              cccGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGc               >  1:2360726/1‑148 (MQ=255)
                                                                                                                              cccGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGc               <  2:811551/148‑1 (MQ=255)
                                                                                                                              cccGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGc               >  1:1187156/1‑148 (MQ=255)
                                                                                                                                cGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCa             >  2:3452465/1‑148 (MQ=255)
                                                                                                                                cGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCa             <  1:3177148/148‑1 (MQ=255)
                                                                                                                                 ggTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCaa            >  2:2777270/1‑148 (MQ=255)
                                                                                                                                 ggTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCaa            <  1:713321/148‑1 (MQ=255)
                                                                                                                                    aGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCg         >  1:2036571/1‑148 (MQ=255)
                                                                                                                                     gCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGc        >  1:3314216/1‑148 (MQ=255)
                                                                                                                                      ccTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCa       >  1:384731/1‑148 (MQ=255)
                                                                                                                                       cTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCaa      <  2:1467262/148‑1 (MQ=255)
                                                                                                                                        ttCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCTGTATTCGGCTTTCCAGAAATTATCGTCAATGGCCTGTTGCAGGGATTCGGCAGAGGTTTTCACCGCCACGCGTTGCTGCTGCGCCATTTTGCCAACCGCtaa     <  2:1228999/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC  >  CP009273/1548074‑1548360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATT  >  CP009273/1548074‑1548364
                                                                                                                                              |                                                                                                                                                    
TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCG                                                                                                                                               <  SRR19973065.856691/150‑1 (MQ=60)
TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCG                                                                                                                                               <  SRR19973065.856580/150‑1 (MQ=60)
 CGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGC                                                                                                                                              >  SRR19973065.312878/1‑150 (MQ=60)
 CGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGC                                                                                                                                              >  SRR19973065.2569066/1‑150 (MQ=60)
 CGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAAGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGC                                                                                                                                              >  SRR19973065.2569770/1‑150 (MQ=60)
    AACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGG                                                                                                                                           >  SRR19973065.604727/1‑150 (MQ=60)
      CAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCT                                                                                                                                         >  SRR19973065.692175/1‑150 (MQ=60)
            CAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATG                                                                                                                                   >  SRR19973065.997325/1‑150 (MQ=60)
                 TTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGT                                                                                                                              <  SRR19973065.1839403/150‑1 (MQ=60)
                    ATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACG                                                                                                                           >  SRR19973065.2927550/1‑150 (MQ=60)
                      AGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCTGGCTTAGATGGAGGTACGGC                                                                                                                         >  SRR19973065.1988212/1‑150 (MQ=60)
                       GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                                                                                        <  SRR19973065.1606430/150‑1 (MQ=60)
                       GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                                                                                        >  SRR19973065.2754744/1‑150 (MQ=60)
                       GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                                                                                        >  SRR19973065.1509246/1‑150 (MQ=60)
                       GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                                                                                        >  SRR19973065.1422928/1‑150 (MQ=60)
                         TCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGT                                                                                                                      >  SRR19973065.1289437/1‑150 (MQ=60)
                         TCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGT                                                                                                                      >  SRR19973065.1245319/1‑150 (MQ=60)
                         TCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGT                                                                                                                      <  SRR19973065.3311861/150‑1 (MQ=60)
                          CCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTA                                                                                                                     <  SRR19973065.2152953/150‑1 (MQ=60)
                           CTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAG                                                                                                                    <  SRR19973065.1940706/150‑1 (MQ=60)
                           CTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAG                                                                                                                    <  SRR19973065.1940683/150‑1 (MQ=60)
                             CCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTC                                                                                                                  <  SRR19973065.632537/150‑1 (MQ=60)
                              CTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCG                                                                                                                 >  SRR19973065.1236460/1‑150 (MQ=60)
                              CTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTAGCCACTACCCAGTATGGATGAGATCTGAAAAAGTGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGTCGCAGGCTTAGATGGAGGTACGGCGTTAGTCG                                                                                                                 >  SRR19973065.1237027/1‑150 (MQ=60)
                                   CGGATGATGTTCTGCATAGCAGGTGCGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTA                                                                                                            <  SRR19973065.2206491/150‑1 (MQ=60)
                                   CGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTA                                                                                                            <  SRR19973065.2206558/150‑1 (MQ=60)
                                    GGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAT                                                                                                           <  SRR19973065.1663904/150‑1 (MQ=60)
                                      ATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTC                                                                                                         >  SRR19973065.1239554/1‑150 (MQ=60)
                                       TGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCG                                                                                                        >  SRR19973065.3149047/1‑150 (MQ=60)
                                          TGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCT                                                                                                     <  SRR19973065.2068981/150‑1 (MQ=60)
                                          TGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCT                                                                                                     <  SRR19973065.2068982/150‑1 (MQ=60)
                                              CTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCC                                                                                                 >  SRR19973065.1406129/1‑150 (MQ=60)
                                              CTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCC                                                                                                 <  SRR19973065.831242/150‑1 (MQ=60)
                                                   TAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAA                                                                                            >  SRR19973065.1014410/1‑150 (MQ=60)
                                                   TAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAA                                                                                            >  SRR19973065.1227315/1‑150 (MQ=60)
                                                     GCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATT                                                                                          <  SRR19973065.1944083/150‑1 (MQ=60)
                                                      CAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTA                                                                                         >  SRR19973065.2168556/1‑150 (MQ=60)
                                                       AGGTGATGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                                                                                        <  SRR19973065.418625/150‑1 (MQ=60)
                                                       AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCTCTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                                                                                        <  SRR19973065.698296/150‑1 (MQ=60)
                                                       AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                                                                                        >  SRR19973065.2318096/1‑150 (MQ=60)
                                                       AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                                                                                        >  SRR19973065.543237/1‑150 (MQ=60)
                                                       AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                                                                                        <  SRR19973065.1324275/150‑1 (MQ=60)
                                                       AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                                                                                        <  SRR19973065.1323950/150‑1 (MQ=60)
                                                        GGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCTGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATC                                                                                       >  SRR19973065.2666135/1‑150 (MQ=60)
                                                        GGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCTGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATC                                                                                       >  SRR19973065.2666187/1‑150 (MQ=60)
                                                          TGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGT                                                                                     <  SRR19973065.781176/150‑1 (MQ=60)
                                                          TGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGT                                                                                     <  SRR19973065.2550111/150‑1 (MQ=60)
                                                             GGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAA                                                                                  <  SRR19973065.577968/150‑1 (MQ=60)
                                                                   TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT                                                                            >  SRR19973065.1039181/1‑150 (MQ=60)
                                                                   TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT                                                                            <  SRR19973065.98504/150‑1 (MQ=60)
                                                                   TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT                                                                            >  SRR19973065.270619/1‑150 (MQ=60)
                                                                   TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT                                                                            >  SRR19973065.2229630/1‑150 (MQ=60)
                                                                    GATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTG                                                                           >  SRR19973065.1426521/1‑150 (MQ=60)
                                                                    GATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTG                                                                           >  SRR19973065.1419426/1‑150 (MQ=60)
                                                                     ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT                                                                          <  SRR19973065.2215603/150‑1 (MQ=60)
                                                                     ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT                                                                          <  SRR19973065.889261/150‑1 (MQ=60)
                                                                     ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT                                                                          <  SRR19973065.889735/150‑1 (MQ=60)
                                                                     ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT                                                                          <  SRR19973065.2388886/150‑1 (MQ=60)
                                                                      TATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT                                                                         <  SRR19973065.1875972/150‑1 (MQ=60)
                                                                         TTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCA                                                                      >  SRR19973065.828361/1‑150 (MQ=60)
                                                                         TTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCA                                                                      >  SRR19973065.816905/1‑150 (MQ=60)
                                                                         TTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCA                                                                      >  SRR19973065.1722489/1‑150 (MQ=60)
                                                                              CGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCT                                                                 >  SRR19973065.255576/1‑150 (MQ=60)
                                                                                CCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTC                                                               <  SRR19973065.1837278/150‑1 (MQ=60)
                                                                                CCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTC                                                               <  SRR19973065.551174/150‑1 (MQ=60)
                                                                                 CACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCG                                                              <  SRR19973065.1243383/150‑1 (MQ=60)
                                                                                 CACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCG                                                              <  SRR19973065.2359558/150‑1 (MQ=60)
                                                                                    TACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCA                                                           <  SRR19973065.2547377/150‑1 (MQ=60)
                                                                                      CCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGA                                                         >  SRR19973065.398128/1‑150 (MQ=60)
                                                                                      CCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGA                                                         >  SRR19973065.398213/1‑150 (MQ=60)
                                                                                        CAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGG                                                       <  SRR19973065.2115089/150‑1 (MQ=60)
                                                                                          GTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTT                                                     <  SRR19973065.2098510/150‑1 (MQ=60)
                                                                                           TATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTT                                                    <  SRR19973065.2676409/150‑1 (MQ=60)
                                                                                            ATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTT                                                   <  SRR19973065.2069228/150‑1 (MQ=60)
                                                                                                ATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACC                                               <  SRR19973065.2494896/150‑1 (MQ=60)
                                                                                                AAGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACC                                               <  SRR19973065.234148/150‑1 (MQ=60)
                                                                                                          AAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTT                                     <  SRR19973065.363913/150‑1 (MQ=60)
                                                                                                            AAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGC                                   >  SRR19973065.147276/1‑150 (MQ=60)
                                                                                                            AAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGC                                   >  SRR19973065.147342/1‑150 (MQ=60)
                                                                                                              AGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTG                                 >  SRR19973065.2960090/1‑150 (MQ=60)
                                                                                                              AGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTG                                 >  SRR19973065.381964/1‑150 (MQ=60)
                                                                                                              AGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTG                                 >  SRR19973065.382251/1‑150 (MQ=60)
                                                                                                              AGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTG                                 <  SRR19973065.1589429/150‑1 (MQ=60)
                                                                                                              AGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTG                                 >  SRR19973065.2960320/1‑150 (MQ=60)
                                                                                                                  AGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGC                             >  SRR19973065.656748/1‑150 (MQ=60)
                                                                                                                   GAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCG                            >  SRR19973065.2818864/1‑150 (MQ=60)
                                                                                                                    AGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGC                           >  SRR19973065.1841185/1‑150 (MQ=60)
                                                                                                                    AGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGC                           >  SRR19973065.3215684/1‑150 (MQ=60)
                                                                                                                    AGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGC                           >  SRR19973065.1841250/1‑150 (MQ=60)
                                                                                                                      GGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCA                         <  SRR19973065.2729893/150‑1 (MQ=60)
                                                                                                                       GAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCAT                        <  SRR19973065.771719/150‑1 (MQ=60)
                                                                                                                         AATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTT                      >  SRR19973065.848620/1‑150 (MQ=60)
                                                                                                                           TAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTG                    <  SRR19973065.1820751/150‑1 (MQ=60)
                                                                                                                           TAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTG                    <  SRR19973065.2913878/150‑1 (MQ=60)
                                                                                                                            AGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGC                   >  SRR19973065.625292/1‑150 (MQ=60)
                                                                                                                            AGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGC                   >  SRR19973065.625192/1‑150 (MQ=60)
                                                                                                                            AGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGC                   >  SRR19973065.1194664/1‑150 (MQ=60)
                                                                                                                            AGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGC                   >  SRR19973065.599590/1‑150 (MQ=60)
                                                                                                                            AGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGC                   >  SRR19973065.2375409/1‑150 (MQ=60)
                                                                                                                             GCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCC                  <  SRR19973065.3027871/150‑1 (MQ=60)
                                                                                                                                CGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAAC               <  SRR19973065.3196352/150‑1 (MQ=60)
                                                                                                                                 GGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACC              <  SRR19973065.718095/150‑1 (MQ=60)
                                                                                                                                  GTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCG             >  SRR19973065.2049622/1‑150 (MQ=60)
                                                                                                                                   TAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGC            >  SRR19973065.3333561/1‑150 (MQ=60)
                                                                                                                                   TAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGC            >  SRR19973065.3334424/1‑150 (MQ=60)
                                                                                                                                    AGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCA           >  SRR19973065.387417/1‑150 (MQ=60)
                                                                                                                                         TCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGC      >  SRR19973065.2499074/1‑150 (MQ=60)
                                                                                                                                            CTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAAT   <  SRR19973065.2312847/150‑1 (MQ=60)
                                                                                                                                            CTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGGCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAAT   <  SRR19973065.2312970/150‑1 (MQ=60)
                                                                                                                                             TTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATT  <  SRR19973065.3108424/150‑1 (MQ=60)
                                                                                                                                              |                                                                                                                                                    
TCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATT  >  CP009273/1548074‑1548364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑