Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,305,135 T→C 100% Y365C (TAC→TGC)  ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,1350TC99.5% 803.6 / ‑5.0 201Y365C (TAC→TGC) ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (98/102);  total (98/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

CACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATT  >  CP009273/2304990‑2305279
                                                                                                                                                 |                                                                                                                                                
      ggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGa                                                                                                                                          >  2:2457848/1‑148 (MQ=255)
      ggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTATGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGa                                                                                                                                          >  1:1297996/1‑148 (MQ=255)
       ttAATGCTTTTTACCATACGTGATTAGCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAc                                                                                                                                         <  1:144094/147‑1 (MQ=255)
            gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCcga                                                                                                                                    >  1:2506881/1‑148 (MQ=255)
            gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCaga                                                                                                                                    >  2:2791490/1‑148 (MQ=255)
             tttATTTCCATACGTGATTATCCTCTTGCGAACGGTCGCACGAGTACACCAAAAAACTGTGTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCagag                                                                                                                                   <  2:958118/147‑1 (MQ=255)
             cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCagag                                                                                                                                   <  2:2737343/148‑1 (MQ=255)
             cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCagag                                                                                                                                   >  1:978385/1‑148 (MQ=255)
             cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCagag                                                                                                                                   >  1:2096880/1‑148 (MQ=255)
              ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGc                                                                                                                                  >  2:2112156/1‑148 (MQ=255)
                aTTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTa                                                                                                                                <  2:3244231/148‑1 (MQ=255)
                aTTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTa                                                                                                                                <  2:3326528/148‑1 (MQ=255)
                   tCCATACGTTTTTATCCTCATGCGAAGGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGa                                                                                                                             <  1:2279002/148‑1 (MQ=255)
                        aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                                        >  1:1499390/1‑148 (MQ=255)
                        aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                                        <  2:3188817/148‑1 (MQ=255)
                         cGTGATTATCCTCATGCGAACGGTCGCAAGATTACACAAAAAAAATGATTTTGTACGCTGAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTAt                                                                                                                       <  1:590216/148‑1 (MQ=255)
                           tGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCa                                                                                                                     >  1:2429889/1‑148 (MQ=255)
                           tGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCa                                                                                                                     >  1:641568/1‑148 (MQ=255)
                               tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCGTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAAGATGTTATCAgtgt                                                                                                                 >  1:530167/1‑148 (MQ=255)
                               tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAgtgt                                                                                                                 <  1:1947440/148‑1 (MQ=255)
                                  ccTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCGTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGa                                                                                                              >  2:879483/1‑148 (MQ=255)
                                   cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                                             >  2:1211427/1‑148 (MQ=255)
                                    tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                                            >  2:672634/1‑148 (MQ=255)
                                    tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                                            <  1:1750145/148‑1 (MQ=255)
                                     cATGCGAACGCTCGCAAGAGTACACCAAAAAACAGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCCGAGCTACGATGTTATCAGTGTTGATGc                                                                                                           <  2:3493474/148‑1 (MQ=255)
                                           aaCGGCCCCAAGAGAACACCAAAAAACTGAGTTTTTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGt                                                                                                     <  1:1045678/148‑1 (MQ=255)
                                               gTCGCAAGATTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg                                                                                                 <  2:1044345/148‑1 (MQ=255)
                                                    aaGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                                                            <  2:3088545/148‑1 (MQ=255)
                                                     agagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                                                           >  1:2860155/1‑148 (MQ=255)
                                                       agTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACt                                                                                         <  2:1418422/148‑1 (MQ=255)
                                                       agTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACt                                                                                         <  1:2576041/148‑1 (MQ=255)
                                                        gTACCCCAAAAAACTGAGTTGGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTg                                                                                        <  1:2484370/148‑1 (MQ=255)
                                                         tACACCAAAAACCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                                       <  1:2553711/148‑1 (MQ=255)
                                                         tACACCAAAAAACTGCGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                                       <  1:765993/148‑1 (MQ=255)
                                                         tACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                                       >  2:2628135/1‑148 (MQ=255)
                                                         tACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                                       >  1:3013344/1‑148 (MQ=255)
                                                          acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                                                                      <  1:2112156/148‑1 (MQ=255)
                                                             ccAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGACCTGGTTgt                                                                                   >  1:815264/1‑148 (MQ=255)
                                                             ccAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                                                   <  1:2334117/148‑1 (MQ=255)
                                                             caaaaaacCTGAGTTTGTACCCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                                                   <  1:2858532/146‑1 (MQ=255)
                                                              cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                                                                  >  2:204874/1‑148 (MQ=255)
                                                                aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                                                >  1:92960/1‑148 (MQ=255)
                                                                aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                                                >  2:1295773/1‑148 (MQ=255)
                                                                aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAAACAGACACAGAGCTAAGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                                                >  1:1897739/1‑148 (MQ=255)
                                                                 aaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                                                               <  1:1701306/148‑1 (MQ=255)
                                                                      tGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACGGGTTGTCGTCcagca                                                                          >  2:3249031/1‑148 (MQ=255)
                                                                         ttttGTAGGCTTAAAACAATGAAAATAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAACCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                                                                       <  1:2593166/147‑1 (MQ=255)
                                                                         gTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                                                                       <  2:2926241/148‑1 (MQ=255)
                                                                          tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                                                               >  1:3391131/1‑139 (MQ=255)
                                                                          tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                                                               <  2:3391131/139‑1 (MQ=255)
                                                                          tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtt                                                                      <  2:2859563/148‑1 (MQ=255)
                                                                           ttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTg                                                                     <  2:2763224/148‑1 (MQ=255)
                                                                            tGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGa                                                                    <  2:161808/148‑1 (MQ=255)
                                                                              tACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtt                                                                  <  1:1890073/148‑1 (MQ=255)
                                                                               aCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                                                                 >  2:2193873/1‑148 (MQ=255)
                                                                                 gCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg                                                               <  1:1011839/148‑1 (MQ=255)
                                                                                   tGAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                                                             <  1:2738112/148‑1 (MQ=255)
                                                                                       aaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCa                                                         <  2:1115731/148‑1 (MQ=255)
                                                                                        aCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa                                                        <  2:641568/148‑1 (MQ=255)
                                                                                        aCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa                                                        <  2:1695118/148‑1 (MQ=255)
                                                                                        aCAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa                                                        <  1:1375852/148‑1 (MQ=255)
                                                                                          aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                      <  1:2913028/148‑1 (MQ=255)
                                                                                            tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                    >  1:2289370/1‑148 (MQ=255)
                                                                                            tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                    >  2:1557739/1‑148 (MQ=255)
                                                                                            tGAAAAAAGGGCCCGCAGGCCCTTTGTACGATATAAATCGATATTAGAACTGGCAAACCAGACCCAGAGCAACGATCTTATCAGGGTTGATGCCAGCGTCACGATTGACCTGGTTGTCGTCCAGCAGCTTGATTTTGTAGTCCACGTa                                                    >  1:2415156/1‑148 (MQ=255)
                                                                                                aaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg                                                >  2:1056627/1‑148 (MQ=255)
                                                                                                 aaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg                                               <  1:2727277/148‑1 (MQ=255)
                                                                                                  aaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                                              >  1:3125903/1‑148 (MQ=255)
                                                                                                   aGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc                                             >  1:1155527/1‑148 (MQ=255)
                                                                                                   aGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc                                             <  2:478249/148‑1 (MQ=255)
                                                                                                   aGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc                                             >  1:3248056/1‑148 (MQ=255)
                                                                                                    gggCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGCCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                                            >  1:394578/1‑148 (MQ=255)
                                                                                                      gCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg                                          <  1:1688607/148‑1 (MQ=255)
                                                                                                      gCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg                                          <  1:2457848/148‑1 (MQ=255)
                                                                                                      gCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg                                          <  1:2032608/148‑1 (MQ=255)
                                                                                                      gCCCGCAGGCCCTTTGTTCGATATAAATCGAGATGAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg                                          <  2:3057288/148‑1 (MQ=255)
                                                                                                         cGCAGGCCCTTTGTTCGATATAACTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt                                       <  2:3158056/148‑1 (MQ=255)
                                                                                                         cGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt                                       <  2:132244/148‑1 (MQ=255)
                                                                                                          gCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                      <  2:1498809/148‑1 (MQ=255)
                                                                                                            aGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                    <  2:2514119/148‑1 (MQ=255)
                                                                                                            aGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                    <  2:725285/148‑1 (MQ=255)
                                                                                                             ggCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                   >  2:3136083/1‑148 (MQ=255)
                                                                                                             ggCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                   >  2:3422994/1‑148 (MQ=255)
                                                                                                             ggCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                   >  1:677092/1‑148 (MQ=255)
                                                                                                              gCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACACAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                                  <  1:3266448/148‑1 (MQ=255)
                                                                                                                ccTTTGTTCGATATAAATCGAGATTAGAACTGGCATACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTATGTGGACATGTTTTTGTTGa                                <  2:1984074/148‑1 (MQ=255)
                                                                                                                      ttCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                          >  1:1817494/1‑148 (MQ=255)
                                                                                                                      ttCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                          >  1:550340/1‑148 (MQ=255)
                                                                                                                       tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta                         <  1:2074452/148‑1 (MQ=255)
                                                                                                                         gATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                       <  1:3422994/148‑1 (MQ=255)
                                                                                                                           tataAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggta                     >  1:3331196/1‑148 (MQ=255)
                                                                                                                             taaaTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGc                   >  1:72818/3‑148 (MQ=255)
                                                                                                                                  cGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATAAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa              >  2:2156069/1‑148 (MQ=255)
                                                                                                                                    agaTTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca            >  2:1712032/1‑148 (MQ=255)
                                                                                                                                       ttAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca         <  1:3214468/148‑1 (MQ=255)
                                                                                                                                       ttAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca         >  2:741467/1‑148 (MQ=255)
                                                                                                                                            aCTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata    <  2:1604327/148‑1 (MQ=255)
                                                                                                                                            aCTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata    <  1:270781/148‑1 (MQ=255)
                                                                                                                                             cTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat   >  2:1534231/1‑148 (MQ=255)
                                                                                                                                              tGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt  >  2:684248/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATT  >  CP009273/2304990‑2305279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGG  >  CP009273/2304992‑2305284
                                                                                                                                                |                                                                                                                                                     
ggggggtaaagattttttcaatagtttATTATCCACATGCGAACGGTCGCAATAGTACACCAAAAAA‑CTGTGTTTGTACGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCA                                                                                                                                                 <  SRR19973066.1101213/123‑1 (MQ=60)
  CGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCGTTGTTCGATATAAATCGAGATTAGAAATGGCAAACCAGA                                                                                                                                               >  SRR19973066.2047153/1‑150 (MQ=60)
  CGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTATGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGA                                                                                                                                               >  SRR19973066.1307399/1‑150 (MQ=60)
    GTTAAAGCTTTTTTCCATACTTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACC                                                                                                                                             <  SRR19973066.2664890/150‑1 (MQ=60)
     ttaatgctttttaCCATACGTGATTAGCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCC                                                                                                                                            <  SRR19973066.145472/137‑1 (MQ=60)
     GTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCC                                                                                                                                            >  SRR19973066.3291536/1‑150 (MQ=60)
      taaatgattatttccttactttatatgcctcatgcgtacggtctatagagtacaaaaaaaaactctttttgtccgattaaaaaaattAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCC                                                                                                                                            <  SRR19973066.1258628/63‑1 (MQ=60)
       AAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAG                                                                                                                                          >  SRR19973066.2537018/1‑150 (MQ=60)
        AAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCCGA                                                                                                                                         >  SRR19973066.2523954/1‑150 (MQ=60)
        AAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGA                                                                                                                                         >  SRR19973066.3219017/1‑150 (MQ=60)
         AGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAG                                                                                                                                        >  SRR19973066.985598/1‑150 (MQ=60)
         AGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAG                                                                                                                                        >  SRR19973066.2111316/1‑150 (MQ=60)
           CTTATTTCCATAGGTTATTATCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTCAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCT                                                                                                                                      <  SRR19973066.2752565/150‑1 (MQ=60)
             taatttcaattagttattatcataagtctaccggaccaaacataacacaaaaaaagtgtgtttttactcttaaaaaaataAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTA                                                                                                                                     <  SRR19973066.348887/70‑1 (MQ=60)
             TATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                                    >  SRR19973066.1029305/1‑150 (MQ=60)
                 TCCATACGTTTTTATCCTCATGCGAAGGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATG                                                                                                                                <  SRR19973066.2294658/150‑1 (MQ=60)
                    ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                                             >  SRR19973066.1509256/1‑150 (MQ=60)
                    ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                                             >  SRR19973066.1509843/1‑150 (MQ=60)
                      ACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATC                                                                                                                           >  SRR19973066.3241399/1‑150 (MQ=60)
                       CGTGATTATCCTCATGCGAACGGTCGCAAGATTACACAAAAAAA‑ATGATTTTGTACGCTGAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                                          <  SRR19973066.594322/150‑1 (MQ=60)
                       CGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                                          >  SRR19973066.2153138/1‑150 (MQ=60)
                       CGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                                          >  SRR19973066.2446418/1‑150 (MQ=60)
                       CGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                                          >  SRR19973066.646208/1‑150 (MQ=60)
                           ATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCGTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAAGATGTTATCAGTGT                                                                                                                      >  SRR19973066.534038/1‑150 (MQ=60)
                            attccccccctgcgaccgcccccaccacaacacaaaaaaacttcgttttcccgctcaaaaaaaacAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGT                                                                                                                      <  SRR19973066.4329/85‑1 (MQ=60)
                            ataaacctctcccgaacggtcgaaatcaaaaaccaaaacactgagttttgacccttAAAACAATAAAAAACGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGT                                                                                                                      <  SRR19973066.4644/94‑1 (MQ=60)
                            TTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTT                                                                                                                     >  SRR19973066.762859/1‑150 (MQ=60)
                             TATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTG                                                                                                                    <  SRR19973066.1961236/150‑1 (MQ=60)
                                 CTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGC                                                                                                                >  SRR19973066.2444611/1‑150 (MQ=60)
                                  TCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCC                                                                                                               <  SRR19973066.1762738/150‑1 (MQ=60)
                                      GCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCG                                                                                                           <  SRR19973066.2321530/150‑1 (MQ=60)
                                         accggccgcacgagaACACCAAAAAA‑CTGAGTTTGTACGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                                        <  SRR19973066.1053414/135‑1 (MQ=60)
                                         AACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                                        >  SRR19973066.1979959/1‑150 (MQ=60)
                                         AACGGCCCCAAGAGAACACCAAAAAA‑CTGAGTTTTTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                                        <  SRR19973066.1053362/150‑1 (MQ=60)
                                             gtctaAAGAGTACACAAAAAAA‑CTGTTTTTTTACGATCAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAG                                                                                                    <  SRR19973066.909133/145‑1 (MQ=60)
                                                 CAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAA                                                                                                >  SRR19973066.2878770/1‑150 (MQ=60)
                                                 CAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAA                                                                                                >  SRR19973066.2879365/1‑150 (MQ=60)
                                                  AAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCGTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAC                                                                                               >  SRR19973066.2124938/1‑150 (MQ=60)
                                                     ATTACACCAAAAAA‑CTGAGTTTGTACGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                                            <  SRR19973066.2499699/150‑1 (MQ=60)
                                                     AGTACACCAAAAAA‑CTGATTTTGTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                                            <  SRR19973066.2593518/150‑1 (MQ=60)
                                                     AGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                                            >  SRR19973066.3033423/1‑150 (MQ=60)
                                                      GTACCCCAAAAAA‑CTGAGTTGGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGT                                                                                           <  SRR19973066.2501319/150‑1 (MQ=60)
                                                       TACCCCAAAAAA‑CTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTT                                                                                          <  SRR19973066.2571080/150‑1 (MQ=60)
                                                       TACACCAAAAAC‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTT                                                                                          <  SRR19973066.2571055/150‑1 (MQ=60)
                                                       TACACCAAAAAA‑CTGCGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTT                                                                                          <  SRR19973066.771666/150‑1 (MQ=60)
                                                        ACACCAAAAAA‑CTGATTTTTTCCCATCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTG                                                                                         <  SRR19973066.1552377/150‑1 (MQ=60)
                                                        ACACCAAAAAA‑CTGAGTTTGAACGCTGAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTG                                                                                         <  SRR19973066.2126749/150‑1 (MQ=60)
                                                         CACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGACCTGGTTGT                                                                                        >  SRR19973066.821238/1‑150 (MQ=60)
                                                         CACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT                                                                                        >  SRR19973066.54723/1‑150 (MQ=60)
                                                          ACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                                       >  SRR19973066.2412258/1‑150 (MQ=60)
                                                           CCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCG                                                                                      <  SRR19973066.2350034/150‑1 (MQ=60)
                                                            CAAAAAACCTGAGTTTGTACCCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCG                                                                                      <  SRR19973066.2877733/150‑1 (MQ=60)
                                                            CAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGT                                                                                     >  SRR19973066.93963/1‑150 (MQ=60)
                                                            CAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGT                                                                                     >  SRR19973066.1913537/1‑150 (MQ=60)
                                                            CAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAAACAGACACAGAGCTAAGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGT                                                                                     >  SRR19973066.1911278/1‑150 (MQ=60)
                                                               AAAA‑CTGAGTTTGTACGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCA                                                                                  <  SRR19973066.1715398/150‑1 (MQ=60)
                                                               AAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCA                                                                                  <  SRR19973066.1893282/150‑1 (MQ=60)
                                                               AAAA‑CTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCA                                                                                  <  SRR19973066.1713663/150‑1 (MQ=60)
                                                                  A‑CTGATTTTGTCCGCTCAAAACAATTAAAAAAGGGCCCGCAGGACCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                                               <  SRR19973066.2091757/150‑1 (MQ=60)
                                                                       AGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAagatcgg                                                                           >  SRR19973066.3413980/1‑143 (MQ=60)
                                                                       AGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAagatcgg                                                                           >  SRR19973066.3413764/1‑143 (MQ=60)
                                                                        TTTTGTAGGCTTAAAACAATGAAAATAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAACCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTG                                                                          <  SRR19973066.2610730/150‑1 (MQ=60)
                                                                           TGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATT                                                                       >  SRR19973066.361282/1‑150 (MQ=60)
                                                                             TACGCTTAAAACAAGGAACACAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTT                                                                     <  SRR19973066.1904335/150‑1 (MQ=60)
                                                                             TACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTT                                                                     <  SRR19973066.1903550/150‑1 (MQ=60)
                                                                               CGCTGAAAACAATGAAAAAAGGGCCCGCCGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGT                                                                   >  SRR19973066.2797365/1‑150 (MQ=60)
                                                                                GCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTA                                                                  <  SRR19973066.1019294/150‑1 (MQ=60)
                                                                                  TGAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGT                                                                <  SRR19973066.2756532/150‑1 (MQ=60)
                                                                                     AAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAA                                                             >  SRR19973066.1511576/1‑150 (MQ=60)
                                                                                       ACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACG                                                           <  SRR19973066.1385805/150‑1 (MQ=60)
                                                                                       ACAAACAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACG                                                           <  SRR19973066.1219914/150‑1 (MQ=60)
                                                                                         AATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                                         <  SRR19973066.2932465/150‑1 (MQ=60)
                                                                                         AATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                                         >  SRR19973066.2305073/1‑150 (MQ=60)
                                                                                         AATGAAAAAAGGGCCCGCAGGCCCTTTGTACGATATAAATCGATATTAGAACTGGCAAACCAGACCCAGAGCAACGATCTTATCAGGGTTGATGCCAGCGTCACGATTGACCTGGTTGTCGTCCAGCAGCTTGATTTTGTAGTCCACGTA                                                         >  SRR19973066.2431516/1‑150 (MQ=60)
                                                                                          ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                                        >  SRR19973066.30150/1‑150 (MQ=60)
                                                                                           TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                                       <  SRR19973066.143896/150‑1 (MQ=60)
                                                                                             AAAAACGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATNTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTG                                                     <  SRR19973066.1821100/150‑1 (MQ=60)
                                                                                               AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                                   >  SRR19973066.3146775/1‑150 (MQ=60)
                                                                                                AAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                                                  <  SRR19973066.2745620/150‑1 (MQ=60)
                                                                                                AAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                                                  >  SRR19973066.1163744/1‑150 (MQ=60)
                                                                                                AAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                                                  >  SRR19973066.3269462/1‑150 (MQ=60)
                                                                                                 AAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGCCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACA                                                 >  SRR19973066.397686/1‑150 (MQ=60)
                                                                                                  AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                                                >  SRR19973066.2897534/1‑150 (MQ=60)
                                                                                                   GGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATG                                               <  SRR19973066.981022/150‑1 (MQ=60)
                                                                                                     GCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTT                                             <  SRR19973066.1700897/150‑1 (MQ=60)
                                                                                                     GCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTT                                             <  SRR19973066.2046794/150‑1 (MQ=60)
                                                                                                     GCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTT                                             <  SRR19973066.2474603/150‑1 (MQ=60)
                                                                                                     GCCCGCAGGCCCTTTGTTCGATATAAATCGAGATGAGAACTGGCAACCCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTT                                             <  SRR19973066.1700917/150‑1 (MQ=60)
                                                                                                      CCCGCAGGCCCTTTGTTCGATATAAAACGAGATTAGAACTGGCAAACCAGACCCAGATCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAAGTTT                                            >  SRR19973066.794174/1‑150 (MQ=60)
                                                                                                          CAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT                                        >  SRR19973066.682049/1‑150 (MQ=60)
                                                                                                          CAGGCCCTTTGTTCGATATAAATCGAGATTAGAAATTGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT                                        >  SRR19973066.681919/1‑150 (MQ=60)
                                                                                                            GGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTG                                      <  SRR19973066.692063/150‑1 (MQ=60)
                                                                                                            GGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTG                                      <  SRR19973066.3392378/150‑1 (MQ=60)
                                                                                                             GCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGA                                     <  SRR19973066.3287880/150‑1 (MQ=60)
                                                                                                             GCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACACAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGA                                     <  SRR19973066.3287910/150‑1 (MQ=60)
                                                                                                               CCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAG                                   >  SRR19973066.3117630/1‑150 (MQ=60)
                                                                                                                   TGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGT                               >  SRR19973066.1830443/1‑150 (MQ=60)
                                                                                                                   TGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGT                               >  SRR19973066.554297/1‑150 (MQ=60)
                                                                                                                      TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG                            <  SRR19973066.2088796/150‑1 (MQ=60)
                                                                                                                        GATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTA                          <  SRR19973066.3445886/150‑1 (MQ=60)
                                                                                                                        GATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTA                          >  SRR19973066.626754/1‑150 (MQ=60)
                                                                                                                        GATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTA                          >  SRR19973066.3353345/1‑150 (MQ=60)
                                                                                                                          TATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGC                        >  SRR19973066.73682/1‑150 (MQ=60)
                                                                                                                          TATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGC                        >  SRR19973066.585770/1‑150 (MQ=60)
                                                                                                                            TAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC                      >  SRR19973066.2513084/1‑150 (MQ=60)
                                                                                                                                      TTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAAC            <  SRR19973066.3235754/150‑1 (MQ=60)
                                                                                                                                        AGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACAT          >  SRR19973066.503035/1‑150 (MQ=60)
                                                                                                                                          AACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAT        >  SRR19973066.2295175/1‑150 (MQ=60)
                                                                                                                                           ACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATT       <  SRR19973066.272786/150‑1 (MQ=60)
                                                                                                                                           ACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATT       <  SRR19973066.259710/150‑1 (MQ=60)
                                                                                                                                           ACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATT       <  SRR19973066.273208/150‑1 (MQ=60)
                                                                                                                                            CTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTT      <  SRR19973066.1329442/150‑1 (MQ=60)
                                                                                                                                            CTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTT      <  SRR19973066.245648/150‑1 (MQ=60)
                                                                                                                                            CTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTT      >  SRR19973066.2982976/1‑150 (MQ=60)
                                                                                                                                             TGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTC     >  SRR19973066.3252371/1‑150 (MQ=60)
                                                                                                                                               GCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAG   >  SRR19973066.2109118/1‑150 (MQ=60)
                                                                                                                                               GCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGAAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAG   >  SRR19973066.1936139/1‑150 (MQ=60)
                                                                                                                                                CAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGG  >  SRR19973066.3394862/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
CGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAA‑CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGG  >  CP009273/2304992‑2305284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑