Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,305,135 T→C 64.3% Y365C (TAC→TGC)  ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,1350TC64.3% 244.4 / 234.1 213Y365C (TAC→TGC) ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base T (41/35);  new base C (64/73);  total (105/108)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 213 total aligned reads displayed.

TGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCA  >  CP009273/2304987‑2305282
                                                                                                                                                     |                                                                                                                                                   
tGCCACGCGGTTAAAGTTTATTTCAATAGTTTATTATCCTCTTGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTTCGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTa                                                                                                                                                    <  1:3143539/150‑1 (MQ=255)
        gggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAc                                                                                                                                            >  2:4723106/1‑150 (MQ=255)
             aaGCTTATGTCCATACGTGATTCTCCTCTTGCGAACGGGAGCAAGAGTAC‑ACCAAAAAACTGATTTTTTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCaga                                                                                                                                       <  2:1156368/150‑1 (MQ=255)
              aGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCagag                                                                                                                                      >  2:343747/1‑150 (MQ=255)
                cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCGTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCt                                                                                                                                    >  2:3332034/1‑150 (MQ=255)
                   aTTTCCATACGTGATTATCCTCATGCGAACGGTCGAAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACg                                                                                                                                 <  1:2771406/150‑1 (MQ=255)
                     ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAt                                                                                                                               >  2:1774090/1‑150 (MQ=255)
                      tCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATg                                                                                                                              >  2:8903/1‑150 (MQ=255)
                              tGATTAACCACAGCCCAACGGTCCAAAGA‑TACAACAAAAAAACTTCGTTTCCACGCTTAAAACATTCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAgt                                                                                                                      <  1:2937185/150‑1 (MQ=255)
                                 ttcccctcctGCGAACGTACGAAATAGAAC‑ACCAAAAACCTGTGTTGGTACGATTAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                                                                   <  1:1279838/146‑1 (MQ=255)
                                 ttATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                                                                   >  2:681858/1‑150 (MQ=255)
                                 ttATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                                                                   >  2:2035273/1‑150 (MQ=255)
                                  tagccTCATGCGATCGGTCGCATGATTAC‑ACCAAAAAACTGTGTTTGTACGATGAAAACTATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTg                                                                                                                  <  2:3074105/147‑1 (MQ=255)
                                  tATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTg                                                                                                                  >  1:2961141/1‑150 (MQ=255)
                                   aTCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGa                                                                                                                 >  1:3456460/1‑150 (MQ=255)
                                   aTCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGa                                                                                                                 >  2:4063272/1‑150 (MQ=255)
                                    tCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                                                >  2:4539302/1‑150 (MQ=255)
                                    accTCATCCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                                                <  1:619186/149‑1 (MQ=255)
                                     ccTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                                               >  2:2555444/1‑150 (MQ=255)
                                       tCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGcc                                                                                                             <  2:3841854/150‑1 (MQ=255)
                                        cATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                                                                            >  1:3059440/1‑150 (MQ=255)
                                        cATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                                                                            >  1:2651865/1‑150 (MQ=255)
                                         aTGCGAACGGTCGCAAGAGTAC‑ACCAAAACACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                                                           <  2:241800/150‑1 (MQ=255)
                                          tGCGAACGGTCGCAAGTGTAC‑ACCAAAAAACTGTGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGc                                                                                                          <  1:2221574/150‑1 (MQ=255)
                                            ctcaCGGCCGCAAGAGCAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAACAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGt                                                                                                        <  2:2535226/147‑1 (MQ=255)
                                            cGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGt                                                                                                        >  1:3640290/1‑150 (MQ=255)
                                             gAACGGTCGCACGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                                                       <  2:76592/150‑1 (MQ=255)
                                             gAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                                                       >  2:4454693/1‑150 (MQ=255)
                                             gAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                                                       <  2:4310533/150‑1 (MQ=255)
                                                   tCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGt                                                                                                 <  2:2673393/150‑1 (MQ=255)
                                                       aaGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc                                                                                             >  1:3966935/1‑150 (MQ=255)
                                                       aaGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc                                                                                             >  2:1475891/1‑150 (MQ=255)
                                                          agTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                                          >  2:3981116/1‑150 (MQ=255)
                                                           gTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                                                                         <  2:66568/150‑1 (MQ=255)
                                                           gTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                                                                         >  2:3513662/1‑150 (MQ=255)
                                                            tAC‑ACCAAAAAACTGAGTTTGAACTCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTATGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGGGTGGATGCCAGCGTCACGAGTGAACTGGtt                                                                                        >  2:1497739/1‑150 (MQ=255)
                                                             ac‑acCAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                                                       <  1:2577912/150‑1 (MQ=255)
                                                              c‑acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                                                      >  1:274524/1‑150 (MQ=255)
                                                                acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTCGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                                                                     <  1:3599756/150‑1 (MQ=255)
                                                                  cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                                                   >  2:4136475/1‑150 (MQ=255)
                                                                  cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                                                   >  2:3899066/1‑150 (MQ=255)
                                                                    aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                                                 >  2:2950232/1‑150 (MQ=255)
                                                                    aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                                                 >  1:1780287/1‑150 (MQ=255)
                                                                    aaaaaCTGAGTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                                                 <  2:1690065/150‑1 (MQ=255)
                                                                     aaaaCTGAGTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCca                                                                                <  1:2154491/150‑1 (MQ=255)
                                                                      aaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                                                               >  2:738660/1‑150 (MQ=255)
                                                                        aCTTAGTTTGTACGCTTAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                                             <  1:3321093/150‑1 (MQ=255)
                                                                         cTGAGTTTGTACGCTGAAAACAATGAAAAAAGTGCCCGCAGGCCCTTTGTTCGATATAAATCGAGAATATAACTGGCAAAACAGACCAACATATAAGATGTTATAAGTGTTGATGAAAGCGTCACGAGTGAACTGGTTGTCGTCaagaag                                                                            >  1:1695340/1‑150 (MQ=255)
                                                                         cTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagcag                                                                            <  2:3389421/150‑1 (MQ=255)
                                                                          tGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAgg                                                                           >  2:282098/1‑150 (MQ=255)
                                                                                   ccGCTCAAAAAAATCAAACAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg                                                                  <  1:2477527/149‑1 (MQ=255)
                                                                                    cGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGt                                                                 >  2:3766709/1‑150 (MQ=255)
                                                                                    cGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGt                                                                 >  1:2890919/1‑150 (MQ=255)
                                                                                     gCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAACTCGAGATGAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                                                                <  1:2996670/150‑1 (MQ=255)
                                                                                        gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                                             >  2:4142311/1‑150 (MQ=255)
                                                                                         aaaaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCa                                                            >  1:1430296/1‑150 (MQ=255)
                                                                                          aaaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTGTGTAGTCaa                                                           >  1:3418117/1‑150 (MQ=255)
                                                                                           aaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAc                                                          <  1:771859/150‑1 (MQ=255)
                                                                                             cAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                        <  2:613257/150‑1 (MQ=255)
                                                                                               aTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                                      >  1:3886401/1‑150 (MQ=255)
                                                                                               aTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATAGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                                      >  1:4551631/1‑150 (MQ=255)
                                                                                                tcaaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                                     <  1:3693859/148‑1 (MQ=255)
                                                                                                 gAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                                                    >  1:479324/1‑150 (MQ=255)
                                                                                                    aaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                                                 <  1:271733/150‑1 (MQ=255)
                                                                                                     aaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc                                                >  2:2389354/1‑150 (MQ=255)
                                                                                                      aaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                                               <  1:2387893/150‑1 (MQ=255)
                                                                                                       aGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                              <  1:4094421/150‑1 (MQ=255)
                                                                                                         ggCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                            <  2:2020721/150‑1 (MQ=255)
                                                                                                         ggCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACGGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                            >  2:4396439/1‑150 (MQ=255)
                                                                                                          gCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt                                           <  2:4425423/150‑1 (MQ=255)
                                                                                                                 ggCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                    <  1:2044884/150‑1 (MQ=255)
                                                                                                                    ccTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                 <  2:1810162/150‑1 (MQ=255)
                                                                                                                      tttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta                               <  2:3145398/150‑1 (MQ=255)
                                                                                                                      tttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta                               <  1:4356492/150‑1 (MQ=255)
                                                                                                                      tttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta                               >  2:3403946/1‑150 (MQ=255)
                                                                                                                         gTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta                            <  1:3208636/150‑1 (MQ=255)
                                                                                                                            cGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGGGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt                         >  2:326010/1‑150 (MQ=255)
                                                                                                                             gATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggta                        >  2:1742093/1‑150 (MQ=255)
                                                                                                                              atatCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                       <  2:2216929/150‑1 (MQ=255)
                                                                                                                              atatAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                       <  1:3030848/150‑1 (MQ=255)
                                                                                                                                ataaaTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                     >  1:1071056/4‑150 (MQ=255)
                                                                                                                                ataaaTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                     <  1:3119622/147‑1 (MQ=255)
                                                                                                                                ataaaTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                     >  1:3524363/4‑150 (MQ=255)
                                                                                                                                ataaaTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                     >  2:2146533/4‑150 (MQ=255)
                                                                                                                                ataaaTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                     >  2:153084/4‑150 (MQ=255)
                                                                                                                                  cAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                   <  2:3001856/150‑1 (MQ=255)
                                                                                                                                  aaaTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                   >  1:3152865/2‑150 (MQ=255)
                                                                                                                                  aaaTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                   <  1:1475891/149‑1 (MQ=255)
                                                                                                                                   aaTCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACca                  >  1:490024/1‑150 (MQ=255)
                                                                                                                                      cGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca               >  1:1429873/1‑150 (MQ=255)
                                                                                                                                       gagaTTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat              <  1:1944265/150‑1 (MQ=255)
                                                                                                                                       gagaTTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat              >  1:356200/1‑150 (MQ=255)
                                                                                                                                         gaTTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca            <  1:1104159/150‑1 (MQ=255)
                                                                                                                                            tAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca         <  1:1801223/150‑1 (MQ=255)
                                                                                                                                             aGAACTGGCAAACCAGACCCAGCGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat        <  2:2760140/150‑1 (MQ=255)
                                                                                                                                              gAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata       <  2:2984562/150‑1 (MQ=255)
                                                                                                                                                aCTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt     >  1:1686774/1‑150 (MQ=255)
                                                                                                                                                 cTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt    <  1:4192563/150‑1 (MQ=255)
                                                                                                                                                  tGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc   >  1:823191/1‑150 (MQ=255)
                                                                                                                                                  tGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAGTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc   >  2:4132416/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTAC‑ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCA  >  CP009273/2304987‑2305282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAG  >  CP009273/2304987‑2305283
                                                                                                                                                    |                                                                                                                                                    
TGCCACGCGGTTAAAGTTTATTTCAATAGTTTATTATCCTCTTGCGAACGGTCGCAAGAGTACACCAAAAAACTTCGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTA                                                                                                                                                     <  SRR19973081.3162744/150‑1 (MQ=60)
TGCCACGCGGTTAAAGCTTATTTCCATACGTTATTAACCTAATGCGAACGGTCGAAAGATTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTA                                                                                                                                                     <  SRR19973081.3161763/150‑1 (MQ=60)
TGCCACGCGGTTAAAGATTATTTCAATACGTGATTATCCTCCTGCGAACGGTCGAAAGATTACACAAAAAAACTGATTTTGTACGCTTAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCA                                                                                                                                                     <  SRR19973081.2172790/150‑1 (MQ=60)
TGCCACGCGGTAAAAGATTATTTCAATACGTGATTATCCTCTTGCGAAGGGTCGAAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCA                                                                                                                                                     <  SRR19973081.2175435/150‑1 (MQ=60)
TGCCACGCGGGTAACGCTTATTACAATAGGTGATTAGCCTCATGCGAACGGTCGAACGAGTACACAAAAAAAGTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCA                                                                                                                                                     <  SRR19973081.2172783/150‑1 (MQ=60)
TGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCTTGCGAACGGTCGAAAGAGTACACCAAAAAACTTAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCA                                                                                                                                                     <  SRR19973081.2823455/150‑1 (MQ=60)
   CACGGGGGTAACGCTTATGACCATACGTGATTAGCCTCCGGCGAAGGGTCGCAAGAGTACACCAAAAAAGTGAGTTTGTCCGCTGAAAACATTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAC                                                                                                                                                  <  SRR19973081.581736/150‑1 (MQ=60)
     CGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCTAACGGACGAAAGAGTACCCCAAAAAACTTATTTTGTACGCTCAAAACATTTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCA                                                                                                                                                <  SRR19973081.3506416/150‑1 (MQ=60)
                   ATTTCCATACGTGATTATCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACG                                                                                                                                  <  SRR19973081.2788385/150‑1 (MQ=60)
                    TTTCCATACGTGATTAGCCTCATGCGACGGGTCGCACGAGTACACCAAAAACCTGTGTTTGTCCGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGA                                                                                                                                 <  SRR19973081.2455022/150‑1 (MQ=60)
                              tgattaaccacagcccaacggtccaaagatacaacaaaaaaacttcgtttccacgcttaaaacattcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGT                                                                                                                       <  SRR19973081.2955302/83‑1 (MQ=60)
                               GATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTG                                                                                                                      >  SRR19973081.4161535/1‑150 (MQ=60)
                                ATTATCCTCATGCGAACGGTCCCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGT                                                                                                                     <  SRR19973081.3166336/150‑1 (MQ=60)
                                 ttcccctcctgcgaacgtacgaaatagaACACCAAAAACCTGTGTTGGTACGATTAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTT                                                                                                                    <  SRR19973081.1288326/122‑1 (MQ=60)
                                  TATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTG                                                                                                                   >  SRR19973081.2979397/1‑150 (MQ=60)
                                   ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                                  >  SRR19973081.3477712/1‑150 (MQ=60)
                                    tccaccggcgacgGGTCGCAAGAGAACACCAAAAAACTGAGTTTGTCCGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                                                 <  SRR19973081.3680980/137‑1 (MQ=60)
                                    TCCTCATGCGACCGGTCGCACGAGTACACCAAAAACCTGAGTTGGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                                                 <  SRR19973081.3072473/150‑1 (MQ=60)
                                    ACCTCATCCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                                                 <  SRR19973081.623512/150‑1 (MQ=60)
                                        CATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCA                                                                                                             >  SRR19973081.3078259/1‑150 (MQ=60)
                                        CATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCA                                                                                                             >  SRR19973081.2668354/1‑150 (MQ=60)
                                          TGCGAACGGTCGCAAGTGTACACCAAAAAACTGTGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGC                                                                                                           <  SRR19973081.2235680/150‑1 (MQ=60)
                                          GGCGACCGGTCCCAAGAGAACACCAAAAAACTGAGTTTGTACTCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGC                                                                                                           <  SRR19973081.911016/150‑1 (MQ=60)
                                            CGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGT                                                                                                         >  SRR19973081.3662250/1‑150 (MQ=60)
                                                     GCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGA                                                                                                <  SRR19973081.1173920/150‑1 (MQ=60)
                                                       AAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAC                                                                                              >  SRR19973081.3991119/1‑150 (MQ=60)
                                                       AAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAC                                                                                              >  SRR19973081.3991163/1‑150 (MQ=60)
                                                       AAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAC                                                                                              >  SRR19973081.4258613/1‑150 (MQ=60)
                                                            TACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTT                                                                                         >  SRR19973081.2766612/1‑150 (MQ=60)
                                                             ACACCAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTG                                                                                        <  SRR19973081.2593963/150‑1 (MQ=60)
                                                              CACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT                                                                                       >  SRR19973081.276676/1‑150 (MQ=60)
                                                               ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                                      <  SRR19973081.3621570/150‑1 (MQ=60)
                                                               ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTCGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                                      <  SRR19973081.3621584/150‑1 (MQ=60)
                                                                   AAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCC                                                                                  >  SRR19973081.1791932/1‑150 (MQ=60)
                                                                    AAAACTGTGTTTTTACGATTAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCA                                                                                 <  SRR19973081.2168239/150‑1 (MQ=60)
                                                                    AAAACTGTGTTTGTACGCTTAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCA                                                                                 <  SRR19973081.2168218/150‑1 (MQ=60)
                                                                    AAAACTGAGTTTGTACGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCA                                                                                 <  SRR19973081.3848450/150‑1 (MQ=60)
                                                                      AACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGC                                                                               >  SRR19973081.1368143/1‑150 (MQ=60)
                                                                       ACTTAGTTTGTACGCTTAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                                              <  SRR19973081.3341414/150‑1 (MQ=60)
                                                                       ACTGTGTTTGAAGGCTTAAAACAAGCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                                              <  SRR19973081.3341558/150‑1 (MQ=60)
                                                                       ACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                                              <  SRR19973081.1740808/150‑1 (MQ=60)
                                                                        CTGAGTTTGTACGCTGAAAACAATGAAAAAAGTGCCCGCAGGCCCTTTGTTCGATATAAATCGAGAATATAACTGGCAAAACAGACCAACATATAAGATGTTATAAGTGTTGATGAAAGCGTCACGAGTGAACTGGTTGTCGTCAAGAAG                                                                             >  SRR19973081.1706447/1‑150 (MQ=60)
                                                                          GAGTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGT                                                                           <  SRR19973081.1797519/150‑1 (MQ=60)
                                                                           AGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTT                                                                          <  SRR19973081.290326/150‑1 (MQ=60)
                                                                                 TACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTT                                                                    >  SRR19973081.3862317/1‑150 (MQ=60)
                                                                                  ccgctcaaaaaaatcaaacAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTG                                                                   <  SRR19973081.2492919/131‑1 (MQ=60)
                                                                                   CGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGT                                                                  >  SRR19973081.2908685/1‑150 (MQ=60)
                                                                                    GCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAACTCGAGATGAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTA                                                                 <  SRR19973081.3015145/150‑1 (MQ=60)
                                                                                     CTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAG                                                                <  SRR19973081.4030805/150‑1 (MQ=60)
                                                                                       GAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTC                                                              >  SRR19973081.3092587/1‑150 (MQ=60)
                                                                                        AAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCA                                                             >  SRR19973081.1439803/1‑150 (MQ=60)
                                                                                        AAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCA                                                             >  SRR19973081.1439865/1‑150 (MQ=60)
                                                                                         AAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGCTTGTGTAGTCAA                                                            >  SRR19973081.3439137/1‑150 (MQ=60)
                                                                                          AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAC                                                           <  SRR19973081.777952/150‑1 (MQ=60)
                                                                                          AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAC                                                           <  SRR19973081.776808/150‑1 (MQ=60)
                                                                                          AACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAC                                                           <  SRR19973081.3451681/150‑1 (MQ=60)
                                                                                           ACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACG                                                          <  SRR19973081.4758867/150‑1 (MQ=60)
                                                                                             AATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                                        >  SRR19973081.3226736/1‑150 (MQ=60)
                                                                                              ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                                       >  SRR19973081.3910285/1‑150 (MQ=60)
                                                                                              ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATAGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                                       >  SRR19973081.4579224/1‑150 (MQ=60)
                                                                                               TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                                      <  SRR19973081.3715531/150‑1 (MQ=60)
                                                                                               TCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                                      <  SRR19973081.3716336/150‑1 (MQ=60)
                                                                                                GAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGT                                                     >  SRR19973081.482697/1‑150 (MQ=60)
                                                                                                   AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                                  >  SRR19973081.1754035/1‑150 (MQ=60)
                                                                                                   AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                                  <  SRR19973081.273135/150‑1 (MQ=60)
                                                                                                   AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                                  <  SRR19973081.273878/150‑1 (MQ=60)
                                                                                                     AAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACA                                                <  SRR19973081.538423/150‑1 (MQ=60)
                                                                                                     AAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACA                                                <  SRR19973081.2402644/150‑1 (MQ=60)
                                                                                                      AGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                                               <  SRR19973081.4119286/150‑1 (MQ=60)
                                                                                                           CCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTT                                          >  SRR19973081.488179/1‑150 (MQ=60)
                                                                                                            CGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTT                                         >  SRR19973081.2162809/1‑150 (MQ=60)
                                                                                                               AGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                                      <  SRR19973081.2642319/150‑1 (MQ=60)
                                                                                                                GGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTG                                     <  SRR19973081.3632737/150‑1 (MQ=60)
                                                                                                                GGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTG                                     <  SRR19973081.2057757/150‑1 (MQ=60)
                                                                                                                  CCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAA                                   >  SRR19973081.153557/1‑150 (MQ=60)
                                                                                                                     TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA                                <  SRR19973081.4382891/150‑1 (MQ=60)
                                                                                                                     TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA                                <  SRR19973081.2229842/150‑1 (MQ=60)
                                                                                                                     TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA                                <  SRR19973081.4383960/150‑1 (MQ=60)
                                                                                                                       TGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGT                              <  SRR19973081.1084866/150‑1 (MQ=60)
                                                                                                                        GTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTA                             <  SRR19973081.3228529/150‑1 (MQ=60)
                                                                                                                        GTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTA                             <  SRR19973081.3228368/150‑1 (MQ=60)
                                                                                                                        GTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTA                             <  SRR19973081.3228039/150‑1 (MQ=60)
                                                                                                                          TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG                           <  SRR19973081.3034539/150‑1 (MQ=60)
                                                                                                                             ATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG                        <  SRR19973081.1813124/150‑1 (MQ=60)
                                                                                                                             ATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG                        <  SRR19973081.3049526/150‑1 (MQ=60)
                                                                                                                               ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA                      >  SRR19973081.1078182/1‑150 (MQ=60)
                                                                                                                               ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA                      >  SRR19973081.3545914/1‑150 (MQ=60)
                                                                                                                               ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA                      <  SRR19973081.3138726/150‑1 (MQ=60)
                                                                                                                                 AAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC                    <  SRR19973081.2574713/150‑1 (MQ=60)
                                                                                                                                 AAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC                    <  SRR19973081.1485673/150‑1 (MQ=60)
                                                                                                                                 AAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC                    >  SRR19973081.3172096/1‑150 (MQ=60)
                                                                                                                                  AATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCA                   >  SRR19973081.493499/1‑150 (MQ=60)
                                                                                                                                  AATCGAGATGAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCA                   <  SRR19973081.2910466/150‑1 (MQ=60)
                                                                                                                                   ATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAA                  <  SRR19973081.2973024/150‑1 (MQ=60)
                                                                                                                                     CGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACA                >  SRR19973081.1439439/1‑150 (MQ=60)
                                                                                                                                     CGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACA                <  SRR19973081.1483925/150‑1 (MQ=60)
                                                                                                                                      GAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACAT               <  SRR19973081.1956588/150‑1 (MQ=60)
                                                                                                                                      GAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACAT               <  SRR19973081.1956591/150‑1 (MQ=60)
                                                                                                                                      GAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACAT               >  SRR19973081.3011588/1‑150 (MQ=60)
                                                                                                                                      GAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACAT               >  SRR19973081.358612/1‑150 (MQ=60)
                                                                                                                                      GAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACAT               >  SRR19973081.358650/1‑150 (MQ=60)
                                                                                                                                        GATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCA             <  SRR19973081.1111432/150‑1 (MQ=60)
                                                                                                                                          TTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAAC           >  SRR19973081.2993286/1‑150 (MQ=60)
                                                                                                                                           TAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACA          <  SRR19973081.1812986/150‑1 (MQ=60)
                                                                                                                                           TAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACA          <  SRR19973081.2515487/150‑1 (MQ=60)
                                                                                                                                               ACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATT      >  SRR19973081.1700965/1‑150 (MQ=60)
                                                                                                                                               ACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATT      >  SRR19973081.1697818/1‑150 (MQ=60)
                                                                                                                                               ACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATT      <  SRR19973081.758180/150‑1 (MQ=60)
                                                                                                                                                CTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTT     <  SRR19973081.4218193/150‑1 (MQ=60)
                                                                                                                                                 TGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTC    <  SRR19973081.2734999/150‑1 (MQ=60)
                                                                                                                                                 TGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTC    >  SRR19973081.828361/1‑150 (MQ=60)
                                                                                                                                                  GGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCA   >  SRR19973081.2337122/1‑150 (MQ=60)
                                                                                                                                                   GCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAG  <  SRR19973081.3456908/150‑1 (MQ=60)
                                                                                                                                                   GCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAG  <  SRR19973081.3456352/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAG  >  CP009273/2304987‑2305283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑