Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,373,985 C→A 100% intergenic (+37/‑117) yhcB → / → degQ DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,373,9850CA100.0% 681.9 / ‑6.7 214intergenic (+37/‑117)yhcB/degQDUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (91/122);  total (91/123)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

TCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  CP009273/3373840‑3374129
                                                                                                                                                 |                                                                                                                                                
tCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatc                                                                                                                                                >  2:2207378/1‑148 (MQ=255)
 cGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatct                                                                                                                                               >  2:580454/1‑148 (MQ=255)
  gTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatctc                                                                                                                                              <  2:359895/148‑1 (MQ=255)
      ggCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATctccctc                                                                                                                                          <  2:3191611/148‑1 (MQ=255)
       gCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATctccctcc                                                                                                                                         <  1:1039182/148‑1 (MQ=255)
       gCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTATGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATctccctcc                                                                                                                                         <  1:1182705/148‑1 (MQ=255)
        cAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCtccctccc                                                                                                                                        <  1:487141/148‑1 (MQ=255)
        cAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCtccctccc                                                                                                                                        <  1:2099331/148‑1 (MQ=255)
         agagTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTcccc                                                                                                                                       <  1:81794/148‑1 (MQ=255)
           agTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGa                                                                                                                                     <  2:2185723/148‑1 (MQ=255)
           agTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGa                                                                                                                                     >  2:1128093/1‑148 (MQ=255)
            gTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGAc                                                                                                                                    <  2:1205221/148‑1 (MQ=255)
               tGAAGCCAGTAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTAt                                                                                                                                 <  1:1620287/148‑1 (MQ=255)
                gAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATc                                                                                                                                >  2:336551/1‑148 (MQ=255)
                gAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATc                                                                                                                                >  2:2134573/1‑148 (MQ=255)
                gAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATc                                                                                                                                <  1:2211168/148‑1 (MQ=255)
                 aaGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCa                                                                                                                               <  1:1938015/148‑1 (MQ=255)
                 aaGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCa                                                                                                                               <  1:134392/148‑1 (MQ=255)
                   gCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACGATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCAtg                                                                                                                             >  1:3299766/1‑147 (MQ=255)
                     cAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTa                                                                                                                           <  1:2520289/148‑1 (MQ=255)
                     cAGCAACGATCAGGCACCGGTGCAGAGGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTa                                                                                                                           <  1:10093/148‑1 (MQ=255)
                       gCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAAt                                                                                                                         <  1:2529734/148‑1 (MQ=255)
                        cAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATc                                                                                                                        <  2:613801/148‑1 (MQ=255)
                        cAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATc                                                                                                                        <  1:3490012/148‑1 (MQ=255)
                        cAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATc                                                                                                                        <  1:2607249/148‑1 (MQ=255)
                          aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTg                                                                                                                      >  1:1039762/1‑148 (MQ=255)
                          aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTg                                                                                                                      >  2:753881/1‑148 (MQ=255)
                          aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTg                                                                                                                      <  2:2903097/148‑1 (MQ=255)
                           cGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTgg                                                                                                                     >  1:1538556/1‑148 (MQ=255)
                           cGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTgg                                                                                                                     <  2:654160/148‑1 (MQ=255)
                           cGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTgg                                                                                                                     <  2:1470115/148‑1 (MQ=255)
                                 ggCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGtctc                                                                                                               >  2:260400/1‑148 (MQ=255)
                                   cACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGCGTCTCAt                                                                                                             <  2:2348339/148‑1 (MQ=255)
                                         tGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAg                                                                                                       >  1:3039245/1‑148 (MQ=255)
                                             gATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGt                                                                                                   <  1:2394649/148‑1 (MQ=255)
                                             gATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGt                                                                                                   >  2:1681256/1‑148 (MQ=255)
                                              aTGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTc                                                                                                  >  2:2025546/1‑148 (MQ=255)
                                              aTGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTc                                                                                                  >  1:1205646/1‑148 (MQ=255)
                                              aTGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTc                                                                                                  <  1:2414878/148‑1 (MQ=255)
                                              aTGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTc                                                                                                  >  2:1366612/1‑148 (MQ=255)
                                                 ccTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                               >  2:505583/1‑148 (MQ=255)
                                                   tCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGaaa                                                                                             >  2:169035/1‑148 (MQ=255)
                                                     gcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAAt                                                                                           <  1:753881/148‑1 (MQ=255)
                                                       gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTc                                                                                         <  1:1243661/148‑1 (MQ=255)
                                                         cTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       >  2:810840/1‑148 (MQ=255)
                                                         cTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       <  1:1499518/148‑1 (MQ=255)
                                                         cTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATATAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       >  2:810827/1‑148 (MQ=255)
                                                             tCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAAc                                                                                   <  2:1937296/148‑1 (MQ=255)
                                                              cTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACa                                                                                  >  1:783862/1‑148 (MQ=255)
                                                                  aGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCaa                                                                              >  1:2752217/1‑148 (MQ=255)
                                                                   ggCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTTATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCaaa                                                                             <  2:2505953/148‑1 (MQ=255)
                                                                    gcgcATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAAc                                                                            <  1:3391171/148‑1 (MQ=255)
                                                                     cgcATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACt                                                                           <  2:2272023/148‑1 (MQ=255)
                                                                         tCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGttt                                                                       <  2:1094977/148‑1 (MQ=255)
                                                                         tCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGttt                                                                       <  2:2874294/148‑1 (MQ=255)
                                                                          ccGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGtttt                                                                      >  1:1445900/1‑148 (MQ=255)
                                                                            ggCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGa                                                                    <  1:3179310/148‑1 (MQ=255)
                                                                              ccTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAt                                                                  <  1:547359/148‑1 (MQ=255)
                                                                               ctgctgCGTACTTGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCATACATTGCGCACTCCTCTTATCTCCCTCCGCGACTATCTTTTATTCTGGTGTCTCTTTGTTATCCGTATTAAAATTCAATAACATCAAACTGTTTTGAAtc                                                                 <  2:1816845/148‑1 (MQ=255)
                                                                                     cGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCtttt                                                           <  2:2228837/148‑1 (MQ=255)
                                                                                        aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCtt                                                        <  2:1653101/148‑1 (MQ=255)
                                                                                        aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATATCTTTTCtt                                                        >  2:2320537/1‑148 (MQ=255)
                                                                                         cTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTa                                                       <  1:948427/148‑1 (MQ=255)
                                                                                          tGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      >  2:381500/1‑148 (MQ=255)
                                                                                           ggCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATc                                                     <  1:1681256/148‑1 (MQ=255)
                                                                                            gcgcGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCa                                                    <  2:1991845/148‑1 (MQ=255)
                                                                                            gcgcGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCa                                                    >  2:1476824/1‑148 (MQ=255)
                                                                                            gcgcGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCa                                                    <  1:2948131/148‑1 (MQ=255)
                                                                                               cgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTc                                                 <  2:420667/148‑1 (MQ=255)
                                                                                                  aGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAgg                                              >  1:2158483/1‑148 (MQ=255)
                                                                                                   gcgcGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGt                                             <  2:2984647/148‑1 (MQ=255)
                                                                                                      cgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACg                                          >  2:1798494/1‑148 (MQ=255)
                                                                                                       gACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACga                                         <  1:923436/148‑1 (MQ=255)
                                                                                                        aCTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACgag                                        >  1:2303630/1‑148 (MQ=255)
                                                                                                         cTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACgaga                                       <  2:3414902/148‑1 (MQ=255)
                                                                                                          tAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGagag                                      <  2:3195544/148‑1 (MQ=255)
                                                                                                          tAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGagag                                      >  1:1733277/1‑148 (MQ=255)
                                                                                                               tatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGa                                 >  1:1294267/1‑148 (MQ=255)
                                                                                                                  ttttCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGaata                              >  2:2959230/1‑148 (MQ=255)
                                                                                                                   tttCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGaataa                             <  2:2035006/148‑1 (MQ=255)
                                                                                                                     tCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATg                           >  2:1419576/1‑148 (MQ=255)
                                                                                                                          cgcAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGGGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaaaaa                      >  2:730854/1‑148 (MQ=255)
                                                                                                                            cAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaac                    <  1:2502274/148‑1 (MQ=255)
                                                                                                                             aGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaaca                   <  1:1476824/148‑1 (MQ=255)
                                                                                                                              gCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaacaa                  <  2:2197058/148‑1 (MQ=255)
                                                                                                                               ccATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaacaaa                 >  1:2455502/1‑148 (MQ=255)
                                                                                                                               ccATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaacaaa                 <  2:1445900/148‑1 (MQ=255)
                                                                                                                                 aTTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAAcc               <  1:2936317/148‑1 (MQ=255)
                                                                                                                                  ttGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGa                          >  1:1683571/1‑136 (MQ=255)
                                                                                                                                  ttGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGa                          <  2:1683571/136‑1 (MQ=255)
                                                                                                                                  ttGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAAccc              <  2:1112460/148‑1 (MQ=255)
                                                                                                                                   tGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCa             <  2:304989/148‑1 (MQ=255)
                                                                                                                                    gcgcCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAg            <  2:3039245/148‑1 (MQ=255)
                                                                                                                                       cccTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTg         >  2:352409/1‑148 (MQ=255)
                                                                                                                                        cctcctCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGt        >  2:3466605/1‑148 (MQ=255)
                                                                                                                                        cctcctCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGt        <  1:2880119/148‑1 (MQ=255)
                                                                                                                                        cctcctCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGt        >  1:701617/1‑148 (MQ=255)
                                                                                                                                          tcctcTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTg      <  1:2919310/148‑1 (MQ=255)
                                                                                                                                              cttatCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTg  <  1:2045747/148‑1 (MQ=255)
                                                                                                                                              cttatCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAGTCAGGTCCGAGAGCAGGACTAATGAAAAAGCAAACCCAGCTGTTGAGTg  >  2:2587869/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  CP009273/3373840‑3374129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCATT  >  CP009273/3373836‑3374133
                                                                                                                                                     |                                                                                                                                                    
GTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTA                                                                                                                                                      <  SRR19973066.1781176/150‑1 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                                   <  SRR19973066.3162945/150‑1 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                                   <  SRR19973066.2440896/150‑1 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                                   >  SRR19973066.3288333/1‑150 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                                   <  SRR19973066.3162856/150‑1 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                                   <  SRR19973066.2312890/150‑1 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                                   <  SRR19973066.3031425/150‑1 (MQ=60)
      GTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCC                                                                                                                                                <  SRR19973066.2303552/150‑1 (MQ=60)
       TCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCT                                                                                                                                               <  SRR19973066.822704/150‑1 (MQ=60)
         TGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCC                                                                                                                                             >  SRR19973066.1482841/1‑150 (MQ=60)
           GCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCC                                                                                                                                           <  SRR19973066.1894984/150‑1 (MQ=60)
           GCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCC                                                                                                                                           <  SRR19973066.1047275/150‑1 (MQ=60)
           GCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCC                                                                                                                                           <  SRR19973066.1046834/150‑1 (MQ=60)
           GCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCC                                                                                                                                           <  SRR19973066.1895283/150‑1 (MQ=60)
           GCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTATGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCC                                                                                                                                           <  SRR19973066.1191867/150‑1 (MQ=60)
           GCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTATGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCC                                                                                                                                           <  SRR19973066.1191012/150‑1 (MQ=60)
            CAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCG                                                                                                                                          >  SRR19973066.1452192/1‑150 (MQ=60)
            CAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCG                                                                                                                                          <  SRR19973066.2113776/150‑1 (MQ=60)
            CAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCG                                                                                                                                          <  SRR19973066.490795/150‑1 (MQ=60)
             AGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGA                                                                                                                                         <  SRR19973066.82721/150‑1 (MQ=60)
               AGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACT                                                                                                                                       <  SRR19973066.948347/150‑1 (MQ=60)
                   TGAAGCCAGTAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCA                                                                                                                                   <  SRR19973066.1632035/150‑1 (MQ=60)
                   TGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCA                                                                                                                                   <  SRR19973066.474792/150‑1 (MQ=60)
                    GAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCAT                                                                                                                                  <  SRR19973066.2226485/150‑1 (MQ=60)
                     AAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATT                                                                                                                                 >  SRR19973066.1541337/1‑150 (MQ=60)
                     AAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATT                                                                                                                                 <  SRR19973066.1951764/150‑1 (MQ=60)
                     AAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATT                                                                                                                                 <  SRR19973066.135705/150‑1 (MQ=60)
                     AAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACGATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATG                                                                                                                                 >  SRR19973066.3321581/1‑150 (MQ=60)
                      AGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTT                                                                                                                                >  SRR19973066.2981916/1‑150 (MQ=60)
                         CAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAAT                                                                                                                             <  SRR19973066.2537428/150‑1 (MQ=60)
                         CAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAAT                                                                                                                             <  SRR19973066.2537896/150‑1 (MQ=60)
                         CAGCAACGATCAGGCACCGGTGCAGAGGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAAT                                                                                                                             <  SRR19973066.10254/150‑1 (MQ=60)
                          AGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATC                                                                                                                            >  SRR19973066.1689234/1‑150 (MQ=60)
                           GCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCT                                                                                                                           <  SRR19973066.2546935/150‑1 (MQ=60)
                           GCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCT                                                                                                                           <  SRR19973066.2547143/150‑1 (MQ=60)
                           GCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCT                                                                                                                           <  SRR19973066.2546934/150‑1 (MQ=60)
                            CAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTG                                                                                                                          <  SRR19973066.3513296/150‑1 (MQ=60)
                            CAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTG                                                                                                                          >  SRR19973066.1047415/1‑150 (MQ=60)
                            CAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTG                                                                                                                          <  SRR19973066.2624876/150‑1 (MQ=60)
                             AACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGG                                                                                                                         >  SRR19973066.1549540/1‑150 (MQ=60)
                               CGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTG                                                                                                                       <  SRR19973066.3181372/150‑1 (MQ=60)
                               CGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTG                                                                                                                       <  SRR19973066.1394506/150‑1 (MQ=60)
                                   CAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTC                                                                                                                   >  SRR19973066.1207456/1‑150 (MQ=60)
                                   CAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTC                                                                                                                   >  SRR19973066.523733/1‑150 (MQ=60)
                                    AGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCA                                                                                                                  >  SRR19973066.1554201/1‑150 (MQ=60)
                                    AGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCA                                                                                                                  >  SRR19973066.1554930/1‑150 (MQ=60)
                                        ACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGT                                                                                                              >  SRR19973066.3441683/1‑150 (MQ=60)
                                          CGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTA                                                                                                            <  SRR19973066.1293602/150‑1 (MQ=60)
                                           GGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAG                                                                                                           >  SRR19973066.3059555/1‑150 (MQ=60)
                                               CAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGT                                                                                                       >  SRR19973066.1506820/1‑150 (MQ=60)
                                                AGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTC                                                                                                      >  SRR19973066.1214050/1‑150 (MQ=60)
                                                 GATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCT                                                                                                     <  SRR19973066.778195/150‑1 (MQ=60)
                                                 GATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCT                                                                                                     <  SRR19973066.2410773/150‑1 (MQ=60)
                                                  ATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTG                                                                                                    <  SRR19973066.2431238/150‑1 (MQ=60)
                                                   TGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGA                                                                                                   >  SRR19973066.2181056/1‑150 (MQ=60)
                                                      CTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAA                                                                                                <  SRR19973066.2070660/150‑1 (MQ=60)
                                                       TCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAAT                                                                                               <  SRR19973066.2965513/150‑1 (MQ=60)
                                                        CGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATT                                                                                              >  SRR19973066.1226761/1‑150 (MQ=60)
                                                         GCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTC                                                                                             <  SRR19973066.758532/150‑1 (MQ=60)
                                                         GCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTC                                                                                             <  SRR19973066.759475/150‑1 (MQ=60)
                                                           GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAA                                                                                           <  SRR19973066.1252567/150‑1 (MQ=60)
                                                             CTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATA                                                                                         <  SRR19973066.1509972/150‑1 (MQ=60)
                                                             CTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATA                                                                                         >  SRR19973066.3132074/1‑150 (MQ=60)
                                                              TATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAA                                                                                        >  SRR19973066.2421831/1‑150 (MQ=60)
                                                               ATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAAC                                                                                       <  SRR19973066.397696/150‑1 (MQ=60)
                                                               ATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAAC                                                                                       <  SRR19973066.78164/150‑1 (MQ=60)
                                                                TTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACA                                                                                      >  SRR19973066.789648/1‑150 (MQ=60)
                                                                 TCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACAT                                                                                     >  SRR19973066.250041/1‑150 (MQ=60)
                                                                    GAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAA                                                                                  >  SRR19973066.2770746/1‑150 (MQ=60)
                                                                    GAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAA                                                                                  >  SRR19973066.2770767/1‑150 (MQ=60)
                                                                        GCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTG                                                                              <  SRR19973066.3413804/150‑1 (MQ=60)
                                                                            ATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTT                                                                          >  SRR19973066.1456181/1‑150 (MQ=60)
                                                                                GGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAT                                                                      <  SRR19973066.3200462/150‑1 (MQ=60)
                                                                                  CCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCT                                                                    <  SRR19973066.551300/150‑1 (MQ=60)
                                                                                     GCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTT                                                                 <  SRR19973066.3413043/150‑1 (MQ=60)
                                                                                       TGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCTCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTT                                                               <  SRR19973066.2460402/150‑1 (MQ=60)
                                                                                         CGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCT                                                             <  SRR19973066.2904499/150‑1 (MQ=60)
                                                                                         CGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCT                                                             <  SRR19973066.569146/150‑1 (MQ=60)
                                                                                             CTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATC                                                         >  SRR19973066.1981725/1‑150 (MQ=60)
                                                                                             CTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATC                                                         <  SRR19973066.955350/150‑1 (MQ=60)
                                                                                              TGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCA                                                        <  SRR19973066.2123491/150‑1 (MQ=60)
                                                                                               GGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAT                                                       <  SRR19973066.1693497/150‑1 (MQ=60)
                                                                                                GCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATT                                                      <  SRR19973066.1031598/150‑1 (MQ=60)
                                                                                                GCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATT                                                      <  SRR19973066.2967697/150‑1 (MQ=60)
                                                                                                    GAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGG                                                  >  SRR19973066.2173475/1‑150 (MQ=60)
                                                                                                      AGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTA                                                >  SRR19973066.1054475/1‑150 (MQ=60)
                                                                                                      AGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTA                                                <  SRR19973066.837695/150‑1 (MQ=60)
                                                                                                          CGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAG                                            >  SRR19973066.2319404/1‑150 (MQ=60)
                                                                                                           GACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGA                                           <  SRR19973066.930100/150‑1 (MQ=60)
                                                                                                           GACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGGACGAGA                                           >  SRR19973066.2934905/1‑150 (MQ=60)
                                                                                                            ACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAG                                          >  SRR19973066.1745793/1‑150 (MQ=60)
                                                                                                             CTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGC                                         <  SRR19973066.2529501/150‑1 (MQ=60)
                                                                                                               AATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAG                                       <  SRR19973066.2626971/150‑1 (MQ=60)
                                                                                                                ATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGG                                      >  SRR19973066.244382/1‑150 (MQ=60)
                                                                                                                 TTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGA                                     >  SRR19973066.1303644/1‑150 (MQ=60)
                                                                                                                 TTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGA                                     >  SRR19973066.1303900/1‑150 (MQ=60)
                                                                                                                  TTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAA                                    >  SRR19973066.2356189/1‑150 (MQ=60)
                                                                                                                         TCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAA                             <  SRR19973066.294389/150‑1 (MQ=60)
                                                                                                                                CAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAA                      <  SRR19973066.2519319/150‑1 (MQ=60)
                                                                                                                                 AGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAA                     <  SRR19973066.1487217/150‑1 (MQ=60)
                                                                                                                                 AGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTAAGGTACGAGAGCAGGAATAATGAAAAAACAAA                     >  SRR19973066.2472233/1‑150 (MQ=60)
                                                                                                                                    CATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAgatcggaag                  >  SRR19973066.1695830/1‑141 (MQ=60)
                                                                                                                                    CATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAgatcggaag                  >  SRR19973066.1695522/1‑141 (MQ=60)
                                                                                                                                     ATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCA                 <  SRR19973066.2955846/150‑1 (MQ=60)
                                                                                                                                          GCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGT            >  SRR19973066.706799/1‑150 (MQ=60)
                                                                                                                                            CCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTG          >  SRR19973066.2216205/1‑150 (MQ=60)
                                                                                                                                            CCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTG          <  SRR19973066.2899412/150‑1 (MQ=60)
                                                                                                                                              TCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAG        <  SRR19973066.2938773/150‑1 (MQ=60)
                                                                                                                                              TCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAG        <  SRR19973066.1700615/150‑1 (MQ=60)
                                                                                                                                                  CTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCA    <  SRR19973066.2059979/150‑1 (MQ=60)
                                                                                                                                                    TATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCATT  >  SRR19973066.3031852/1‑150 (MQ=60)
                                                                                                                                                    TATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCATT  >  SRR19973066.2110751/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCATT  >  CP009273/3373836‑3374133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑