Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,373,985 C→A intergenic (+37/‑117) yhcB → / → degQ DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,373,9850CA100.0% 749.1 / NA 234intergenic (+37/‑117)yhcB/degQDUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (85/149);  total (85/149)

Only 100 of 241 total aligned reads displayed.

AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  CP009273/3373838‑3374129
                                                                                                                                                   |                                                                                                                                                
aaTCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatc                                                                                                                                                >  1:1337717/1‑150 (MQ=255)
 aTCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatct                                                                                                                                               >  1:1686946/1‑150 (MQ=255)
     tCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATctccct                                                                                                                                           <  1:2014425/150‑1 (MQ=255)
       tGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATctccctcc                                                                                                                                         <  1:612270/150‑1 (MQ=255)
        ggCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCtccctccc                                                                                                                                        >  1:2824558/1‑150 (MQ=255)
           agagTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGa                                                                                                                                     >  1:3799937/1‑150 (MQ=255)
             agTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACt                                                                                                                                   <  1:3074371/150‑1 (MQ=255)
               tCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTAt                                                                                                                                 <  2:3773927/150‑1 (MQ=255)
                  gAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCAt                                                                                                                              >  1:1427920/1‑150 (MQ=255)
                   aaGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCAtt                                                                                                                             <  1:3685907/150‑1 (MQ=255)
                     gCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATCTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTa                                                                                                                           <  1:1711540/150‑1 (MQ=255)
                      ccAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTaa                                                                                                                          <  2:662746/150‑1 (MQ=255)
                       cAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAAt                                                                                                                         >  1:2364684/1‑150 (MQ=255)
                        aGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATc                                                                                                                        <  1:862227/150‑1 (MQ=255)
                          cAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTg                                                                                                                      >  2:1491858/1‑150 (MQ=255)
                            aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgt                                                                                                                    <  2:3438667/150‑1 (MQ=255)
                            aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgt                                                                                                                    >  2:2168035/1‑150 (MQ=255)
                            aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCACTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgt                                                                                                                    >  2:257835/1‑150 (MQ=255)
                               aTCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGtc                                                                                                                 <  2:3153104/150‑1 (MQ=255)
                                   ggCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCAt                                                                                                             <  1:1635661/150‑1 (MQ=255)
                                    gCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCAtt                                                                                                            >  2:400587/1‑150 (MQ=255)
                                     cACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTg                                                                                                           >  1:1753036/1‑150 (MQ=255)
                                     cACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTg                                                                                                           <  2:1599694/150‑1 (MQ=255)
                                      aCCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGt                                                                                                          <  1:1857424/150‑1 (MQ=255)
                                         ggTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTTTTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAg                                                                                                       <  1:244602/150‑1 (MQ=255)
                                           tGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGcc                                                                                                     <  1:541793/150‑1 (MQ=255)
                                           tGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGcc                                                                                                     <  2:1583761/150‑1 (MQ=255)
                                            gCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCg                                                                                                    <  1:3041543/150‑1 (MQ=255)
                                              aGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTc                                                                                                  >  2:1176039/1‑150 (MQ=255)
                                                 tGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                               >  1:3447104/1‑150 (MQ=255)
                                                 tGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                               <  2:1499842/150‑1 (MQ=255)
                                                 tGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                               <  1:106667/150‑1 (MQ=255)
                                                   ccTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGaaa                                                                                             <  1:3649814/150‑1 (MQ=255)
                                                    cTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGaaaa                                                                                            >  1:1156631/1‑150 (MQ=255)
                                                      cgcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAAtt                                                                                          <  1:2127541/150‑1 (MQ=255)
                                                      cgcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAAtt                                                                                          <  1:3530345/150‑1 (MQ=255)
                                                       gcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTc                                                                                         <  2:3243490/150‑1 (MQ=255)
                                                       gcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTc                                                                                         <  2:3243459/150‑1 (MQ=255)
                                                        cgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCa                                                                                        >  1:49044/1‑150 (MQ=255)
                                                         gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       >  2:1946151/1‑150 (MQ=255)
                                                         gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       >  1:997978/1‑150 (MQ=255)
                                                         gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       <  1:2416872/150‑1 (MQ=255)
                                                         gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       >  1:803910/1‑150 (MQ=255)
                                                           cTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATa                                                                                     <  2:2051927/150‑1 (MQ=255)
                                                             aTTCTGCAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTACTTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAAc                                                                                   <  2:2835883/150‑1 (MQ=255)
                                                              ttCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACa                                                                                  <  2:2912698/150‑1 (MQ=255)
                                                               tCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACAt                                                                                 <  2:383239/150‑1 (MQ=255)
                                                                  gAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCaa                                                                              <  2:1627873/150‑1 (MQ=255)
                                                                     ggCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTTATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTATCCGTGTGAAAATTCAATAACATCAAACt                                                                           <  1:3571136/150‑1 (MQ=255)
                                                                      gcgcATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTg                                                                          <  2:3382458/150‑1 (MQ=255)
                                                                       cgcATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGt                                                                         <  2:2355936/150‑1 (MQ=255)
                                                                         cATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGttt                                                                       <  1:1485252/150‑1 (MQ=255)
                                                                         cATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGttt                                                                       <  2:1747927/150‑1 (MQ=255)
                                                                          aTCCGGCCTGCTGCGTACTGGCGNGAAGCGNGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTANCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCNTCTGAAAATTCAATAACATCAAACTGtttt                                                                      >  2:2417729/1‑150 (MQ=255)
                                                                          aTCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGtttt                                                                      <  1:3232064/150‑1 (MQ=255)
                                                                             cGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGaa                                                                   >  1:1514249/1‑150 (MQ=255)
                                                                              ggCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAt                                                                  <  2:3570366/150‑1 (MQ=255)
                                                                                   gctgcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCtt                                                             <  2:574155/150‑1 (MQ=255)
                                                                                     tgcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCtttt                                                           >  2:2397270/1‑150 (MQ=255)
                                                                                      gcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTc                                                          <  1:3404273/150‑1 (MQ=255)
                                                                                        gTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCtt                                                        <  2:1897570/150‑1 (MQ=255)
                                                                                         tACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTa                                                       >  1:1606780/1‑150 (MQ=255)
                                                                                         tACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTa                                                       <  2:1670303/150‑1 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      <  1:3001327/150‑1 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      >  1:2876364/1‑150 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      <  2:2267725/150‑1 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      >  1:974047/1‑150 (MQ=255)
                                                                                             ggCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAt                                                   <  1:1577562/150‑1 (MQ=255)
                                                                                             ggCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAt                                                   <  1:3183803/150‑1 (MQ=255)
                                                                                             ggCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAt                                                   <  2:1222667/150‑1 (MQ=255)
                                                                                              gcgcGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAtt                                                  <  2:2727396/150‑1 (MQ=255)
                                                                                               cgcgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTc                                                 <  2:1998450/150‑1 (MQ=255)
                                                                                               cgcgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTc                                                 <  2:2659869/150‑1 (MQ=255)
                                                                                               cgcgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTc                                                 >  2:629546/1‑150 (MQ=255)
                                                                                                 cgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAg                                               >  2:3519709/1‑150 (MQ=255)
                                                                                                 cgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAg                                               >  1:253803/1‑150 (MQ=255)
                                                                                                  gAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAgg                                              <  2:734060/150‑1 (MQ=255)
                                                                                                  gAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAgg                                              >  2:3674550/1‑150 (MQ=255)
                                                                                                   aaGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGt                                             <  2:1337717/150‑1 (MQ=255)
                                                                                                     gcgcGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTAc                                           <  2:375135/150‑1 (MQ=255)
                                                                                                      cgcgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACg                                          <  2:826659/150‑1 (MQ=255)
                                                                                                       gcgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACga                                         <  2:3195862/150‑1 (MQ=255)
                                                                                                        cgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACgag                                        <  1:1974005/150‑1 (MQ=255)
                                                                                                             aaTTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAg                                   <  2:1737900/150‑1 (MQ=255)
                                                                                                             aaTTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAg                                   >  1:2184365/1‑150 (MQ=255)
                                                                                                              atttatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAgg                                  <  1:1056609/150‑1 (MQ=255)
                                                                                                              atttatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAgg                                  >  2:611255/1‑150 (MQ=255)
                                                                                                                ttatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGaa                                >  2:1059529/1‑150 (MQ=255)
                                                                                                                       tCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaa                         <  2:1032401/150‑1 (MQ=255)
                                                                                                                       tCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaa                         <  2:2062585/150‑1 (MQ=255)
                                                                                                                       tCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaa                         >  1:1197593/1‑150 (MQ=255)
                                                                                                                        cGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaaa                        >  1:2454194/1‑150 (MQ=255)
                                                                                                                               aGCCAATGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTATTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaacaaa                 >  1:1286778/1‑150 (MQ=255)
                                                                                                                                   aTTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCa             <  2:2336747/150‑1 (MQ=255)
                                                                                                                                     tGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGc           <  1:3504703/150‑1 (MQ=255)
                                                                                                                                     tGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGc           <  1:2287748/150‑1 (MQ=255)
                                                                                                                                     tGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGc           >  2:2138248/1‑150 (MQ=255)
                                                                                                                                      gcgcCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCt          <  2:1686946/150‑1 (MQ=255)
                                                                                                                                            tcctcTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAg    <  1:2948423/150‑1 (MQ=255)
                                                                                                                                              ctctTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTg  <  1:3357712/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  CP009273/3373838‑3374129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  CP009273/3373836‑3374129
                                                                                                                                                     |                                                                                                                                                
GTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTA                                                                                                                                                  <  SRR19973061.1101963/150‑1 (MQ=60)
GTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTA                                                                                                                                                  <  SRR19973061.1102488/150‑1 (MQ=60)
GTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTA                                                                                                                                                  <  SRR19973061.923201/150‑1 (MQ=60)
 TAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTAT                                                                                                                                                 >  SRR19973061.376152/1‑150 (MQ=60)
  AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGACGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATC                                                                                                                                                >  SRR19973061.1341083/1‑150 (MQ=60)
  AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATC                                                                                                                                                >  SRR19973061.2107716/1‑150 (MQ=60)
  AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATC                                                                                                                                                <  SRR19973061.1494180/150‑1 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                               <  SRR19973061.3662848/150‑1 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                               >  SRR19973061.1691185/1‑150 (MQ=60)
   ATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCT                                                                                                                                               >  SRR19973061.1370217/1‑150 (MQ=60)
     CGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCC                                                                                                                                             >  SRR19973061.3770287/1‑150 (MQ=60)
      GTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCC                                                                                                                                            <  SRR19973061.2441905/150‑1 (MQ=60)
      GTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCC                                                                                                                                            <  SRR19973061.530534/150‑1 (MQ=60)
      GTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCC                                                                                                                                            >  SRR19973061.1618700/1‑150 (MQ=60)
       TCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCT                                                                                                                                           <  SRR19973061.2019485/150‑1 (MQ=60)
       TCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCT                                                                                                                                           <  SRR19973061.1605686/150‑1 (MQ=60)
         TGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCC                                                                                                                                         <  SRR19973061.613845/150‑1 (MQ=60)
          GGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCC                                                                                                                                        >  SRR19973061.2831796/1‑150 (MQ=60)
           GCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCC                                                                                                                                       <  SRR19973061.1538719/150‑1 (MQ=60)
             AGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGA                                                                                                                                     >  SRR19973061.3809622/1‑150 (MQ=60)
               AGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACT                                                                                                                                   <  SRR19973061.3082305/150‑1 (MQ=60)
                 TCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTAT                                                                                                                                 <  SRR19973061.2283869/150‑1 (MQ=60)
                    GAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCAT                                                                                                                              >  SRR19973061.1431514/1‑150 (MQ=60)
                     AAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATT                                                                                                                             <  SRR19973061.3695331/150‑1 (MQ=60)
                      AGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTT                                                                                                                            >  SRR19973061.3066086/1‑150 (MQ=60)
                      AGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTT                                                                                                                            <  SRR19973061.1597776/150‑1 (MQ=60)
                       GCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTA                                                                                                                           <  SRR19973061.1715400/150‑1 (MQ=60)
                       GCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATCTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTA                                                                                                                           <  SRR19973061.1715838/150‑1 (MQ=60)
                         CAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAAT                                                                                                                         >  SRR19973061.2370696/1‑150 (MQ=60)
                          AGCCACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATC                                                                                                                        <  SRR19973061.96688/150‑1 (MQ=60)
                          AGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATC                                                                                                                        <  SRR19973061.864466/150‑1 (MQ=60)
                             AACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGG                                                                                                                     >  SRR19973061.2171809/1‑150 (MQ=60)
                                  TCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCT                                                                                                                <  SRR19973061.2514439/150‑1 (MQ=60)
                                   CAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTC                                                                                                               <  SRR19973061.1484052/150‑1 (MQ=60)
                                     GGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCAT                                                                                                             <  SRR19973061.1639757/150‑1 (MQ=60)
                                     GGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCAT                                                                                                             <  SRR19973061.3027819/150‑1 (MQ=60)
                                       CACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTG                                                                                                           >  SRR19973061.1757434/1‑150 (MQ=60)
                                        ACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGT                                                                                                          <  SRR19973061.1862062/150‑1 (MQ=60)
                                           GGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTTTTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAG                                                                                                       <  SRR19973061.245298/150‑1 (MQ=60)
                                             TGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCC                                                                                                     >  SRR19973061.838029/1‑150 (MQ=60)
                                             TGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCC                                                                                                     >  SRR19973061.2934775/1‑150 (MQ=60)
                                             TGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCC                                                                                                     <  SRR19973061.543219/150‑1 (MQ=60)
                                              GCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCG                                                                                                    <  SRR19973061.3049409/150‑1 (MQ=60)
                                                   TGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGA                                                                                               >  SRR19973061.3455952/1‑150 (MQ=60)
                                                   TGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGA                                                                                               <  SRR19973061.107022/150‑1 (MQ=60)
                                                   TGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGA                                                                                               <  SRR19973061.106968/150‑1 (MQ=60)
                                                     CCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAA                                                                                             <  SRR19973061.3659138/150‑1 (MQ=60)
                                                      CTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAA                                                                                            >  SRR19973061.1159564/1‑150 (MQ=60)
                                                       TCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAAT                                                                                           <  SRR19973061.3464620/150‑1 (MQ=60)
                                                        CGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATT                                                                                          <  SRR19973061.2132874/150‑1 (MQ=60)
                                                        CGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATT                                                                                          <  SRR19973061.2132883/150‑1 (MQ=60)
                                                        CGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATT                                                                                          <  SRR19973061.3539383/150‑1 (MQ=60)
                                                        CGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATT                                                                                          <  SRR19973061.3539385/150‑1 (MQ=60)
                                                          CGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCA                                                                                        >  SRR19973061.49237/1‑150 (MQ=60)
                                                          CGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCA                                                                                        <  SRR19973061.391252/150‑1 (MQ=60)
                                                           GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAA                                                                                       >  SRR19973061.806018/1‑150 (MQ=60)
                                                           GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAA                                                                                       >  SRR19973061.1000522/1‑150 (MQ=60)
                                                           GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAA                                                                                       <  SRR19973061.2422907/150‑1 (MQ=60)
                                                           GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAA                                                                                       >  SRR19973061.1001853/1‑150 (MQ=60)
                                                           GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAA                                                                                       <  SRR19973061.1677426/150‑1 (MQ=60)
                                                           GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAA                                                                                       <  SRR19973061.2423043/150‑1 (MQ=60)
                                                            ACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAAT                                                                                      >  SRR19973061.3284825/1‑150 (MQ=60)
                                                               ATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAAC                                                                                   >  SRR19973061.718076/1‑150 (MQ=60)
                                                                  CTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATC                                                                                <  SRR19973061.3153082/150‑1 (MQ=60)
                                                                   TGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCA                                                                               <  SRR19973061.2833089/150‑1 (MQ=60)
                                                                      AGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAAC                                                                            <  SRR19973061.1218179/150‑1 (MQ=60)
                                                                       GGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTTATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTATCCGTGTGAAAATTCAATAACATCAAACT                                                                           <  SRR19973061.3580287/150‑1 (MQ=60)
                                                                       GGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACT                                                                           <  SRR19973061.1699857/150‑1 (MQ=60)
                                                                           CATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTT                                                                       <  SRR19973061.1488977/150‑1 (MQ=60)
                                                                            ATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTT                                                                      <  SRR19973061.3240372/150‑1 (MQ=60)
                                                                            ATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTT                                                                      <  SRR19973061.1805270/150‑1 (MQ=60)
                                                                               CGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAA                                                                   >  SRR19973061.1518037/1‑150 (MQ=60)
                                                                                GGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAT                                                                  >  SRR19973061.2460317/1‑150 (MQ=60)
                                                                                GGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAT                                                                  >  SRR19973061.1319852/1‑150 (MQ=60)
                                                                                GGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAT                                                                  >  SRR19973061.1314628/1‑150 (MQ=60)
                                                                                    TGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCT                                                              <  SRR19973061.2419468/150‑1 (MQ=60)
                                                                                     GCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTT                                                             <  SRR19973061.835216/150‑1 (MQ=60)
                                                                                        GCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTC                                                          <  SRR19973061.3413047/150‑1 (MQ=60)
                                                                                        GCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTC                                                          <  SRR19973061.3413017/150‑1 (MQ=60)
                                                                                         CGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCT                                                         <  SRR19973061.3017699/150‑1 (MQ=60)
                                                                                           TACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTA                                                       >  SRR19973061.1610779/1‑150 (MQ=60)
                                                                                            ACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAT                                                      <  SRR19973061.3009074/150‑1 (MQ=60)
                                                                                            ACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAT                                                      >  SRR19973061.2883741/1‑150 (MQ=60)
                                                                                            ACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAT                                                      >  SRR19973061.976524/1‑150 (MQ=60)
                                                                                               GGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAT                                                   <  SRR19973061.3191973/150‑1 (MQ=60)
                                                                                               GGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAT                                                   <  SRR19973061.3192242/150‑1 (MQ=60)
                                                                                               GGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAT                                                   >  SRR19973061.3108860/1‑150 (MQ=60)
                                                                                               GGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAT                                                   <  SRR19973061.1581482/150‑1 (MQ=60)
                                                                                                   CGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAG                                               >  SRR19973061.254507/1‑150 (MQ=60)
                                                                                                          CGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAG                                        <  SRR19973061.1978944/150‑1 (MQ=60)
                                                                                                             CTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGC                                     >  SRR19973061.3369414/1‑150 (MQ=60)
                                                                                                               AATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAG                                   >  SRR19973061.2189860/1‑150 (MQ=60)
                                                                                                               AATTGATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAG                                   <  SRR19973061.1104835/150‑1 (MQ=60)
                                                                                                                ATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGG                                  <  SRR19973061.1059323/150‑1 (MQ=60)
                                                                                                                  TTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAA                                <  SRR19973061.287272/150‑1 (MQ=60)
                                                                                                                   TATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAAT                               <  SRR19973061.1981318/150‑1 (MQ=60)
                                                                                                                         TCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTATCCGTCTGAAAAATCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACAAGAACAGGAATAATGAA                         >  SRR19973061.1200548/1‑150 (MQ=60)
                                                                                                                         TCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAA                         <  SRR19973061.3510138/150‑1 (MQ=60)
                                                                                                                         TCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAA                         >  SRR19973061.1200609/1‑150 (MQ=60)
                                                                                                                          CGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAA                        >  SRR19973061.2461421/1‑150 (MQ=60)
                                                                                                                          CGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAA                        >  SRR19973061.2460449/1‑150 (MQ=60)
                                                                                                                            GGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAA                      >  SRR19973061.1845588/1‑150 (MQ=60)
                                                                                                                             GCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAA                     <  SRR19973061.1495601/150‑1 (MQ=60)
                                                                                                                               GCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATTAAAAAACA                   >  SRR19973061.3706474/1‑150 (MQ=60)
                                                                                                                                CAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAA                  >  SRR19973061.984166/1‑150 (MQ=60)
                                                                                                                                 AGCCAATGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTATTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAA                 >  SRR19973061.1289967/1‑150 (MQ=60)
                                                                                                                                  GCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAAC                >  SRR19973061.516051/1‑150 (MQ=60)
                                                                                                                                   CCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACC               <  SRR19973061.610501/150‑1 (MQ=60)
                                                                                                                                    CATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCC              <  SRR19973061.1969047/150‑1 (MQ=60)
                                                                                                                                     ATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCA             <  SRR19973061.1736496/150‑1 (MQ=60)
                                                                                                                                       TGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGC           <  SRR19973061.3513670/150‑1 (MQ=60)
                                                                                                                                       TGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGC           <  SRR19973061.2293534/150‑1 (MQ=60)
                                                                                                                                        GCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCT          <  SRR19973061.2251551/150‑1 (MQ=60)
                                                                                                                                           CCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTT       >  SRR19973061.364443/1‑150 (MQ=60)
                                                                                                                                           CCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTT       <  SRR19973061.2620165/150‑1 (MQ=60)
                                                                                                                                           CCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTT       >  SRR19973061.364497/1‑150 (MQ=60)
                                                                                                                                            CCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTG      <  SRR19973061.1179018/150‑1 (MQ=60)
                                                                                                                                              TCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAG    <  SRR19973061.2333552/150‑1 (MQ=60)
                                                                                                                                              TCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAG    <  SRR19973061.2956022/150‑1 (MQ=60)
                                                                                                                                              TCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAG    >  SRR19973061.993342/1‑150 (MQ=60)
                                                                                                                                                CTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  <  SRR19973061.2995150/150‑1 (MQ=60)
                                                                                                                                                CTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  <  SRR19973061.3366349/150‑1 (MQ=60)
                                                                                                                                                CTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  <  SRR19973061.3366382/150‑1 (MQ=60)
                                                                                                                                                CTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  SRR19973061.3577749/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                
GTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  CP009273/3373836‑3374129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑