Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,727,580 C→A 100% A167A (GCC→GCA)  xylH → D‑xylose ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,727,5800CA98.0% 468.7 / 1.1 151A167A (GCC→GCA) xylHD‑xylose ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (78/70);  total (79/72)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 158 total aligned reads displayed.

GGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACC  >  CP009273/3727436‑3727724
                                                                                                                                                |                                                                                                                                                
gtgACTTCTTGTTGGTGGATGGAACGGATGGTGGGTCGCGTACCGTAAAGTCACTTCATTTATTGTCACCCTCTCGGGCATTTTGGCTTTTCGCGGCATACTCTTTGGCATCACAAACGGCACGACTGTATCCCCCACcagcgcagcg                                                                                                                                               <  1:80498/146‑1 (MQ=255)
gggACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACcagcgcagcg                                                                                                                                               <  1:1518668/148‑1 (MQ=255)
  gACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACCACAGTATCCCCCACCAGCGCAGCGaa                                                                                                                                             >  2:1663408/1‑147 (MQ=255)
   aCTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATg                                                                                                                                            >  2:1905442/1‑148 (MQ=255)
   aCTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATg                                                                                                                                            <  1:1872811/148‑1 (MQ=255)
   aCTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATg                                                                                                                                            <  1:1872743/148‑1 (MQ=255)
      gCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTca                                                                                                                                         >  2:3304520/1‑148 (MQ=255)
         tctcGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACaa                                                                                                                                      >  2:2351578/1‑148 (MQ=255)
          ctcGGTGCCTGTAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGTAATGGTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACaaa                                                                                                                                     <  2:2455709/148‑1 (MQ=255)
            cGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAAtt                                                                                                                                   >  2:2503178/1‑148 (MQ=255)
             ggTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTg                                                                                                                                  <  1:2694113/148‑1 (MQ=255)
             ggTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTg                                                                                                                                  <  1:2693577/148‑1 (MQ=255)
                  cTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCaa                                                                                                                             <  1:1868412/148‑1 (MQ=255)
                  cTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCaa                                                                                                                             <  2:3496711/148‑1 (MQ=255)
                  cTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCaa                                                                                                                             <  1:1067539/148‑1 (MQ=255)
                     gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                          <  2:2183564/148‑1 (MQ=255)
                     gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                          <  2:1699394/148‑1 (MQ=255)
                     gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                          >  1:2852275/1‑148 (MQ=255)
                          gATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTAtct                                                                                                                     >  2:3255907/1‑148 (MQ=255)
                           aTGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTAtctc                                                                                                                    >  1:2268968/1‑148 (MQ=255)
                            tggtggGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTcc                                                                                                                   >  1:1870550/1‑148 (MQ=255)
                            tggtggGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTAGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTcc                                                                                                                   >  1:125929/1‑148 (MQ=255)
                             ggtggGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTccc                                                                                                                  >  1:670241/1‑148 (MQ=255)
                              gtggGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGCCATACTCATTGGCATCACCAACGGCACGACGGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTcccc                                                                                                                 >  2:937403/1‑148 (MQ=255)
                                gggTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGc                                                                                                               <  2:200744/148‑1 (MQ=255)
                                 ggTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGcc                                                                                                              <  1:3539990/148‑1 (MQ=255)
                                      cgTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTAc                                                                                                         <  2:670241/148‑1 (MQ=255)
                                        tACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCg                                                                                                       >  2:2269770/1‑148 (MQ=255)
                                           cGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCt                                                                                                    >  1:2734372/1‑148 (MQ=255)
                                            gTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCtt                                                                                                   <  2:2087976/148‑1 (MQ=255)
                                             tAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtc                                                                                                  >  1:67041/1‑148 (MQ=255)
                                             tAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtc                                                                                                  >  2:324778/1‑148 (MQ=255)
                                               aaGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcat                                                                                                >  2:2746052/1‑148 (MQ=255)
                                                aGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatc                                                                                               >  2:738782/1‑148 (MQ=255)
                                                 gTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatca                                                                                              <  2:2376440/148‑1 (MQ=255)
                                                 gTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatca                                                                                              <  1:1284554/148‑1 (MQ=255)
                                                  tCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTcatcat                                                                                             <  1:2242451/148‑1 (MQ=255)
                                                    ccTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTg                                                                                           >  2:1007349/1‑148 (MQ=255)
                                                    ccTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTg                                                                                           <  2:871811/148‑1 (MQ=255)
                                                     cTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTgg                                                                                          >  1:953964/1‑148 (MQ=255)
                                                     cTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTgg                                                                                          >  1:2002693/1‑148 (MQ=255)
                                                     cTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTgg                                                                                          >  1:1559888/1‑148 (MQ=255)
                                                          tttATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGgcgcgc                                                                                     >  1:3004734/1‑148 (MQ=255)
                                                            tATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCtt                                                                                   <  2:1275616/148‑1 (MQ=255)
                                                            tATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATAATTGGCGCGCtt                                                                                   <  2:107366/148‑1 (MQ=255)
                                                              ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTgg                                                                                 <  1:1142987/148‑1 (MQ=255)
                                                              ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTgg                                                                                 >  1:214116/1‑148 (MQ=255)
                                                              ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTgg                                                                                 >  2:2812375/1‑148 (MQ=255)
                                                                  cACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTa                                                                             >  2:229449/1‑148 (MQ=255)
                                                                  cACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTa                                                                             <  2:2980107/148‑1 (MQ=255)
                                                                   aCCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTaa                                                                            >  1:2056605/1‑148 (MQ=255)
                                                                   aCCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTaa                                                                            >  1:2487716/1‑148 (MQ=255)
                                                                      cTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATgg                                                                         <  1:3227962/148‑1 (MQ=255)
                                                                       tCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGc                                                                        >  2:53244/1‑148 (MQ=255)
                                                                           gggCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCtttt                                                                    <  1:1339813/148‑1 (MQ=255)
                                                                           gggCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCtttt                                                                    <  1:1339835/148‑1 (MQ=255)
                                                                               aTGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttgttg                                                                <  2:1538406/148‑1 (MQ=255)
                                                                                tGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                               <  2:2382938/148‑1 (MQ=255)
                                                                                tGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                               <  2:593412/148‑1 (MQ=255)
                                                                                tGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                               >  1:1451076/1‑148 (MQ=255)
                                                                                tGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                               >  1:1712434/1‑148 (MQ=255)
                                                                                  ttGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttggtt                                                             >  1:103627/1‑148 (MQ=255)
                                                                                   tGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCtt                                                                              >  2:2591276/1‑130 (MQ=255)
                                                                                   tGGCATTTCGCGGAATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCtt                                                                              <  1:2591276/130‑1 (MQ=255)
                                                                                    ggCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGttggttgg                                                           <  1:1036642/148‑1 (MQ=255)
                                                                                     gCATTTCGCGGCATACTCATTGGCATCACCACCGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCGTGGCTTAATGGCTTTTGTTGGTTGGc                                                          <  2:2725988/148‑1 (MQ=255)
                                                                                     gCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGc                                                          >  2:1848574/1‑148 (MQ=255)
                                                                                      cATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCa                                                         <  1:1661845/148‑1 (MQ=255)
                                                                                      cATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCa                                                         >  1:2604308/1‑148 (MQ=255)
                                                                                        tttCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAAt                                                       >  1:2140728/1‑148 (MQ=255)
                                                                                         ttCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATg                                                      <  1:946528/148‑1 (MQ=255)
                                                                                         ttCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATg                                                      <  2:2950460/148‑1 (MQ=255)
                                                                                                     tCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAAt                                          >  1:804034/1‑148 (MQ=255)
                                                                                                         tGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgcgc                                      >  2:2348683/1‑148 (MQ=255)
                                                                                                         tGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGACAGTACCGGCTTCATCATTGGCGAGCGTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgcgc                                      <  1:2845571/148‑1 (MQ=255)
                                                                                                         cggCATCCCCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACACATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgcgc                                      <  1:3493303/147‑1 (MQ=255)
                                                                                                             aTCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTc                                  <  1:3297803/148‑1 (MQ=255)
                                                                                                              tCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCa                                 <  2:1586100/148‑1 (MQ=255)
                                                                                                              tCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCa                                 >  1:1998571/1‑148 (MQ=255)
                                                                                                              tCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCa                                 >  1:2397689/1‑148 (MQ=255)
                                                                                                               cACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAg                                >  1:3446172/1‑148 (MQ=255)
                                                                                                                        cACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGt                       <  1:2962727/148‑1 (MQ=255)
                                                                                                                        cACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGt                       <  2:1451076/148‑1 (MQ=255)
                                                                                                                        cACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGt                       <  1:2351578/148‑1 (MQ=255)
                                                                                                                           gACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTa                    >  2:3026106/1‑148 (MQ=255)
                                                                                                                            aCTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTAc                   >  2:878634/1‑148 (MQ=255)
                                                                                                                             cTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACa                  >  2:2976565/1‑148 (MQ=255)
                                                                                                                             cTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACa                  >  2:2976647/1‑148 (MQ=255)
                                                                                                                              tGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAg                 <  1:485239/148‑1 (MQ=255)
                                                                                                                               gTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt                <  1:3304520/148‑1 (MQ=255)
                                                                                                                               gTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGATTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt                >  2:1823518/1‑148 (MQ=255)
                                                                                                                               gTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGATTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt                >  2:2766277/1‑148 (MQ=255)
                                                                                                                               gTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGATTCATCATTGGCGCGCTTGGCTTAATGGCTGTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt                >  2:2766842/1‑148 (MQ=255)
                                                                                                                                tATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGtc               <  2:51635/148‑1 (MQ=255)
                                                                                                                                 aTCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGtct              >  2:135424/1‑148 (MQ=255)
                                                                                                                                 aTCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGtct              >  1:857700/1‑148 (MQ=255)
                                                                                                                                  tCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGtctc             <  2:3008554/148‑1 (MQ=255)
                                                                                                                                        accaGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCct       >  1:1029902/1‑148 (MQ=255)
                                                                                                                                            gcgcAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTAc   >  2:2861164/1‑148 (MQ=255)
                                                                                                                                            gcgcAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTAc   >  1:344452/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACC  >  CP009273/3727436‑3727724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGT  >  CP009273/3727436‑3727729
                                                                                                                                                |                                                                                                                                                     
gtgacttcttgttggtggaTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCACTTCATTTATTGTCACCCTCTCGGGCATTTTGGCTTTTCGCGGCATACTCTTTGGCATCACAAACGGCACGACTGTATCCCCCACCAGCGCAGCGAT                                                                                                                                                  <  SRR19973066.81416/131‑1 (MQ=60)
GGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGAT                                                                                                                                                  <  SRR19973066.1529358/150‑1 (MQ=60)
GGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGAT                                                                                                                                                  <  SRR19973066.81517/150‑1 (MQ=60)
 GGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATG                                                                                                                                                 <  SRR19973066.3262692/150‑1 (MQ=60)
  GACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGT                                                                                                                                                <  SRR19973066.114421/150‑1 (MQ=60)
   ACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTC                                                                                                                                               <  SRR19973066.1886076/150‑1 (MQ=60)
   ACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTC                                                                                                                                               <  SRR19973066.1886116/150‑1 (MQ=60)
   ACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTC                                                                                                                                               <  SRR19973066.1886184/150‑1 (MQ=60)
    CTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCA                                                                                                                                              >  SRR19973066.1332975/1‑150 (MQ=60)
             GGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGG                                                                                                                                     <  SRR19973066.2711572/150‑1 (MQ=60)
             GGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGG                                                                                                                                     <  SRR19973066.2712114/150‑1 (MQ=60)
                  CTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAG                                                                                                                                <  SRR19973066.1075367/150‑1 (MQ=60)
                  CTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAG                                                                                                                                <  SRR19973066.1881760/150‑1 (MQ=60)
                   TGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGC                                                                                                                               >  SRR19973066.2871442/1‑150 (MQ=60)
                         GGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTC                                                                                                                         >  SRR19973066.2376186/1‑150 (MQ=60)
                         GGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTC                                                                                                                         <  SRR19973066.1358018/150‑1 (MQ=60)
                         GGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTC                                                                                                                         >  SRR19973066.2284573/1‑150 (MQ=60)
                          GATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCC                                                                                                                        >  SRR19973066.1883910/1‑150 (MQ=60)
                          GATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTAGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCC                                                                                                                        >  SRR19973066.127178/1‑150 (MQ=60)
                           ATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCACGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCC                                                                                                                       >  SRR19973066.675135/1‑150 (MQ=60)
                                 GGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAG                                                                                                                 <  SRR19973066.3563511/150‑1 (MQ=60)
                                         ACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCT                                                                                                         >  SRR19973066.2752763/1‑150 (MQ=60)
                                          CCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTT                                                                                                        >  SRR19973066.52294/1‑150 (MQ=60)
                                           CGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTC                                                                                                       >  SRR19973066.67855/1‑150 (MQ=60)
                                                AGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCAT                                                                                                  >  SRR19973066.1562179/1‑150 (MQ=60)
                                                 GTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATT                                                                                                 <  SRR19973066.1293830/150‑1 (MQ=60)
                                                 GTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATT                                                                                                 <  SRR19973066.1293797/150‑1 (MQ=60)
                                                 GTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATT                                                                                                 >  SRR19973066.949980/1‑150 (MQ=60)
                                                 GTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATT                                                                                                 <  SRR19973066.2718962/150‑1 (MQ=60)
                                                  TCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTG                                                                                                <  SRR19973066.2257920/150‑1 (MQ=60)
                                                  TCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTG                                                                                                >  SRR19973066.995758/1‑150 (MQ=60)
                                                   CCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGG                                                                                               >  SRR19973066.960936/1‑150 (MQ=60)
                                                   CCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGG                                                                                               >  SRR19973066.3485821/1‑150 (MQ=60)
                                                   CCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGG                                                                                               >  SRR19973066.2016757/1‑150 (MQ=60)
                                                   CCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGG                                                                                               >  SRR19973066.1571112/1‑150 (MQ=60)
                                                       TCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCG                                                                                           <  SRR19973066.2682868/150‑1 (MQ=60)
                                                        CATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGC                                                                                          >  SRR19973066.3024718/1‑150 (MQ=60)
                                                         ATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCT                                                                                         <  SRR19973066.2152819/150‑1 (MQ=60)
                                                         ATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCT                                                                                         <  SRR19973066.1986720/150‑1 (MQ=60)
                                                            TATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGG                                                                                      >  SRR19973066.215882/1‑150 (MQ=60)
                                                              TTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCT                                                                                    <  SRR19973066.1151151/150‑1 (MQ=60)
                                                                 tgtgatcttcagatcTGTTGGCATTTCGCGGAATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAA                                                                                 <  SRR19973066.2608834/135‑1 (MQ=60)
                                                                 TCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAA                                                                                 >  SRR19973066.2504691/1‑150 (MQ=60)
                                                                 TCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAA                                                                                 >  SRR19973066.2070877/1‑150 (MQ=60)
                                                                   ACCCTCGCGGGCATGTTGGCATTTCGCGGCAGACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATG                                                                               <  SRR19973066.1874914/150‑1 (MQ=60)
                                                                      CTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCT                                                                            <  SRR19973066.3249298/150‑1 (MQ=60)
                                                                       TCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTT                                                                           <  SRR19973066.2561821/150‑1 (MQ=60)
                                                                         GCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTT                                                                         <  SRR19973066.2943902/150‑1 (MQ=60)
                                                                           GGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGT                                                                       <  SRR19973066.1349538/150‑1 (MQ=60)
                                                                           GGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGT                                                                       <  SRR19973066.1349560/150‑1 (MQ=60)
                                                                             GCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTG                                                                     <  SRR19973066.2244396/150‑1 (MQ=60)
                                                                              CATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGG                                                                    >  SRR19973066.1461373/1‑150 (MQ=60)
                                                                              CATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGG                                                                    >  SRR19973066.1724855/1‑150 (MQ=60)
                                                                               ATGTTGGCATTTCGCGGCATACTCATTGGCATGACCAACGTCACGACTGTATCCCCCACCAGCGCAGCGTTGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTTGT                                                                   <  SRR19973066.2305815/150‑1 (MQ=60)
                                                                                TGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTT                                                                  >  SRR19973066.104718/1‑150 (MQ=60)
                                                                                    GGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCA                                                              <  SRR19973066.1044209/150‑1 (MQ=60)
                                                                                    GGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCA                                                              <  SRR19973066.1044268/150‑1 (MQ=60)
                                                                                    GGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCA                                                              >  SRR19973066.2621924/1‑150 (MQ=60)
                                                                                      CATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAAT                                                            >  SRR19973066.2155583/1‑150 (MQ=60)
                                                                                      CATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAAT                                                            <  SRR19973066.1673953/150‑1 (MQ=60)
                                                                                         TTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGC                                                         <  SRR19973066.953441/150‑1 (MQ=60)
                                                                                                  TACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCAGTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAA                                                >  SRR19973066.1689139/1‑150 (MQ=60)
                                                                                                   ACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAAT                                               >  SRR19973066.809941/1‑150 (MQ=60)
                                                                                                         TGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCG                                         <  SRR19973066.2863972/150‑1 (MQ=60)
                                                                                                         TGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGACAGTACCGGCTTCATCATTGGCGAGCGTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCG                                         <  SRR19973066.2864693/150‑1 (MQ=60)
                                                                                                         CGGCATCCCCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACACATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCG                                         <  SRR19973066.3516602/150‑1 (MQ=60)
                                                                                                            CATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCA                                      >  SRR19973066.2413856/1‑150 (MQ=60)
                                                                                                            CATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCA                                      >  SRR19973066.2012622/1‑150 (MQ=60)
                                                                                                            CATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCA                                      >  SRR19973066.2012635/1‑150 (MQ=60)
                                                                                                             ATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAG                                     >  SRR19973066.3469222/1‑150 (MQ=60)
                                                                                                             ATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAG                                     <  SRR19973066.3319598/150‑1 (MQ=60)
                                                                                                                        CACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTT                          <  SRR19973066.2982341/150‑1 (MQ=60)
                                                                                                                        CACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTT                          <  SRR19973066.2367550/150‑1 (MQ=60)
                                                                                                                              TGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTC                    <  SRR19973066.488882/150‑1 (MQ=60)
                                                                                                                               GTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCT                   <  SRR19973066.3326386/150‑1 (MQ=60)
                                                                                                                               GTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCT                   >  SRR19973066.863883/1‑150 (MQ=60)
                                                                                                                                      CCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCT            >  SRR19973066.1037488/1‑150 (MQ=60)
                                                                                                                                      CCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCGTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCT            <  SRR19973066.3057735/150‑1 (MQ=60)
                                                                                                                                          CAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTAC        >  SRR19973066.347121/1‑150 (MQ=60)
                                                                                                                                           AGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGTCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACC       >  SRR19973066.385056/1‑150 (MQ=60)
                                                                                                                                           AGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGTCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACC       >  SRR19973066.3398855/1‑150 (MQ=60)
                                                                                                                                           AGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACC       >  SRR19973066.721368/1‑150 (MQ=60)
                                                                                                                                                AGCGATGTCACAACTTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCGTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGT  <  SRR19973066.944223/150‑1 (MQ=60)
                                                                                                                                                AGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTCTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGT  >  SRR19973066.255356/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
GGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGT  >  CP009273/3727436‑3727729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑