Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F394 I0 R1	1342	215.0	7134094	96.2%	6862998	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,150,054	G→T	100%	S222Y (TCT→TAT)	sthA ←	pyridine nucleotide transhydrogenase, soluble

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,150,054	0	G	T	98.3%	764.1 / 2.9	239	S222Y (TCT→TAT)	sthA	pyridine nucleotide transhydrogenase, soluble
Reads supporting (aligned to +/- strand): ref base G (4/0); new base T (124/111); total (128/111)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGT  >  CP009273/4149909‑4150198

aCCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATaa                                                                                                                                                <  1:2599631/148‑1 (MQ=255)

 ccGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAAt                                                                                                                                               <  1:1860450/148‑1 (MQ=255)

     tGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGa                                                                                                                                           <  2:3379982/148‑1 (MQ=255)

      ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGAc                                                                                                                                          <  2:2223637/148‑1 (MQ=255)

      ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGAc                                                                                                                                          <  2:2223572/148‑1 (MQ=255)

       gCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACa                                                                                                                                         >  2:1691187/1‑148 (MQ=255)

       gCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACa                                                                                                                                         <  2:2080832/148‑1 (MQ=255)

        cATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGACGTGAGAGGAGAGATAATCTGACAt                                                                                                                                        >  2:2692765/1‑148 (MQ=255)

        cATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAt                                                                                                                                        >  2:2731625/1‑148 (MQ=255)

        cATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAt                                                                                                                                        <  1:1790551/148‑1 (MQ=255)

           agagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAtctc                                                                                                                                     >  1:2622298/1‑148 (MQ=255)

            gagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATctct                                                                                                                                    >  1:2078695/1‑148 (MQ=255)

             agCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATACTCTGACATCTCtt                                                                                                                                   >  2:1823631/1‑148 (MQ=255)

              gcaggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTg                                                                                                                                  >  2:191117/1‑148 (MQ=255)

               caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                 >  1:2471912/1‑148 (MQ=255)

               caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                 <  2:2043207/148‑1 (MQ=255)

                aggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGAt                                                                                                                                <  1:3180088/148‑1 (MQ=255)

                 ggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATc                                                                                                                               >  1:3386157/1‑148 (MQ=255)

                  gcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCg                                                                                                                              <  2:2376562/148‑1 (MQ=255)

                  gcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCg                                                                                                                              <  2:2915988/148‑1 (MQ=255)

                    agTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCgag                                                                                                                            <  1:214806/148‑1 (MQ=255)

                    agTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCgag                                                                                                                            <  1:214297/148‑1 (MQ=255)

                    agTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCgag                                                                                                                            <  2:635128/148‑1 (MQ=255)

                      tCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGaa                                                                                                                          >  1:3314869/1‑148 (MQ=255)

                       cAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGGCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGaaa                                                                                                                         >  1:3334555/1‑148 (MQ=255)

                         gCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATg                                                                                                                       >  1:1852507/1‑148 (MQ=255)

                          cTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGc                                                                                                                      >  1:1594709/1‑148 (MQ=255)

                          cTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGc                                                                                                                      <  2:2927734/148‑1 (MQ=255)

                          cTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGc                                                                                                                      >  1:3036454/1‑148 (MQ=255)

                              cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagc                                                                                                                  <  1:911614/148‑1 (MQ=255)

                               aGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagca                                                                                                                 >  2:938115/1‑148 (MQ=255)

                                gTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagcag                                                                                                                >  1:971964/1‑148 (MQ=255)

                                 ttttttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagcagt                                                                                                               >  2:2480779/1‑147 (MQ=255)

                                 ttttttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAgg                                                                                                               <  2:3208637/148‑1 (MQ=255)

                                    tttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa                                                                                                            <  2:17093/148‑1 (MQ=255)

                                    tttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa                                                                                                            <  1:3221625/148‑1 (MQ=255)

                                    tttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa                                                                                                            <  2:3158289/148‑1 (MQ=255)

                                       aCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcg                                                                                                         >  1:2292611/1‑148 (MQ=255)

                                       aCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcg                                                                                                         >  1:499818/1‑148 (MQ=255)

                                       aCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcg                                                                                                         >  2:1127907/1‑148 (MQ=255)

                                         cagACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcgcg                                                                                                       <  1:2557429/146‑1 (MQ=255)

                                          cGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg                                                                                                      >  2:1028542/1‑148 (MQ=255)

                                          cGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg                                                                                                      >  1:519282/1‑148 (MQ=255)

                                          cGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg                                                                                                      <  2:2013029/148‑1 (MQ=255)

                                            aCTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGTAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgt                                                                                                    <  2:429736/148‑1 (MQ=255)

                                             cTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg                                                                                                   >  1:3457054/1‑148 (MQ=255)

                                             cTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg                                                                                                   <  1:536015/148‑1 (MQ=255)

                                              ttCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtgt                                                                                                  >  2:533405/1‑148 (MQ=255)

                                                   aTGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATc                                                                                             >  2:3565584/1‑148 (MQ=255)

                                                     gCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGcc                                                                                                                     >  1:2827696/1‑122 (MQ=255)

                                                     gCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAg                                                                                           <  2:688747/148‑1 (MQ=255)

                                                     gCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAg                                                                                           >  2:2014842/1‑148 (MQ=255)

                                                     gCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAg                                                                                           >  1:3203918/1‑148 (MQ=255)

                                                      cATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGa                                                                                          >  2:2648858/1‑148 (MQ=255)

                                                         gATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATca                                                                                       >  1:1657526/1‑147 (MQ=255)

                                                          aTCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCa                                                                                      >  1:489358/1‑148 (MQ=255)

                                                          aTCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCa                                                                                      <  1:1398009/148‑1 (MQ=255)

                                                            cacaCCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                    >  1:672053/1‑148 (MQ=255)

                                                            cacaCCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                    <  2:2592871/148‑1 (MQ=255)

                                                                 cATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTAc                                                                               >  1:1735235/1‑148 (MQ=255)

                                                                      tCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCa                                                                          <  2:2318343/148‑1 (MQ=255)

                                                                       cacaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAt                                                                         >  1:240384/1‑148 (MQ=255)

                                                                        acaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATa                                                                        <  1:2578515/148‑1 (MQ=255)

                                                                        acaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATa                                                                        <  1:1828432/148‑1 (MQ=255)

                                                                            ccTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACcgc                                                                    >  1:1323296/1‑148 (MQ=255)

                                                                               tCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGa                                                                 <  1:856216/148‑1 (MQ=255)

                                                                                  aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGa                                                              <  2:2803648/148‑1 (MQ=255)

                                                                                  aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGa                                                              >  2:1047756/1‑148 (MQ=255)

                                                                                    cttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATc                                                            >  2:2627517/1‑148 (MQ=255)

                                                                                        tcGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACg                                                        <  2:1642139/148‑1 (MQ=255)

                                                                                        tcGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACg                                                        >  1:3503276/1‑148 (MQ=255)

                                                                                         cGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGc                                                       <  1:3071114/148‑1 (MQ=255)

                                                                                           tACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat                                                     >  2:2548823/1‑148 (MQ=255)

                                                                                           tACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat                                                     <  2:1381101/148‑1 (MQ=255)

                                                                                                 tCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcac                                               >  2:3324949/1‑148 (MQ=255)

                                                                                                 tCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcac                                               >  1:1581267/1‑148 (MQ=255)

                                                                                                     tgtgACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGcc                                           <  1:93043/148‑1 (MQ=255)

                                                                                                       tgACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGa                                         >  1:389514/1‑148 (MQ=255)

                                                                                                          cGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCa                                      >  2:52752/1‑148 (MQ=255)

                                                                                                            aaTCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACt                                    >  2:3447007/1‑148 (MQ=255)

                                                                                                             aTCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTc                                   >  2:2821635/1‑148 (MQ=255)

                                                                                                             aTCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTc                                   <  1:2047854/148‑1 (MQ=255)

                                                                                                               cACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCa                                 >  2:1244197/1‑148 (MQ=255)

                                                                                                                aCTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAg                                >  1:1182479/1‑148 (MQ=255)

                                                                                                                aCTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAg                                >  1:3273713/1‑148 (MQ=255)

                                                                                                                   aCGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                             >  2:839790/1‑148 (MQ=255)

                                                                                                                          tGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGAt                      <  1:3101585/148‑1 (MQ=255)

                                                                                                                            ttCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATaa                    <  2:1521080/148‑1 (MQ=255)

                                                                                                                            ttCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATaa                    <  2:2249502/148‑1 (MQ=255)

                                                                                                                              ccAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGt                  <  1:2482485/148‑1 (MQ=255)

                                                                                                                                 gAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACa               <  1:1699326/148‑1 (MQ=255)

                                                                                                                                 gAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACa               <  1:3565584/148‑1 (MQ=255)

                                                                                                                                 gAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTAAAGACAGACCGCGGAATCTAGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACa               <  1:3497566/148‑1 (MQ=255)

                                                                                                                                    gTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATgg            <  1:111363/148‑1 (MQ=255)

                                                                                                                                     tGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGgc           >  1:1023362/1‑148 (MQ=255)

                                                                                                                                       aTAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGgcgc         >  2:2144945/1‑148 (MQ=255)

                                                                                                                                        tAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGcgcg        >  1:2246149/1‑148 (MQ=255)

                                                                                                                                         aGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCgg       <  2:2620379/148‑1 (MQ=255)

                                                                                                                                          ggAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGt      >  1:2926215/1‑148 (MQ=255)

                                                                                                                                              agataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCgt  <  2:2078695/148‑1 (MQ=255)

ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGT  >  CP009273/4149909‑4150198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGC  >  CP009273/4149905‑4150203

TGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAT                                                                                                                                                       <  SRR19973066.722851/150‑1 (MQ=60)

  CGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAA                                                                                                                                                     >  SRR19973066.2616248/1‑150 (MQ=60)

    ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATC                                                                                                                                                   <  SRR19973066.2617225/150‑1 (MQ=60)

    ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATC                                                                                                                                                   <  SRR19973066.339345/150‑1 (MQ=60)

     CCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCT                                                                                                                                                  <  SRR19973066.1873750/150‑1 (MQ=60)

      CGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTG                                                                                                                                                 >  SRR19973066.1391086/1‑150 (MQ=60)

        TTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGAC                                                                                                                                               <  SRR19973066.373404/150‑1 (MQ=60)

        TTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGAC                                                                                                                                               <  SRR19973066.373273/150‑1 (MQ=60)

            CATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCT                                                                                                                                           <  SRR19973066.1803305/150‑1 (MQ=60)

             ATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTC                                                                                                                                          >  SRR19973066.2639989/1‑150 (MQ=60)

              TAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCT                                                                                                                                         >  SRR19973066.2092322/1‑150 (MQ=60)

              TAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCT                                                                                                                                         >  SRR19973066.2093055/1‑150 (MQ=60)

              TAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGCTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCT                                                                                                                                         >  SRR19973066.2093148/1‑150 (MQ=60)

               AGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTT                                                                                                                                        >  SRR19973066.2617866/1‑150 (MQ=60)

                 AGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGA                                                                                                                                      <  SRR19973066.2750383/150‑1 (MQ=60)

                 AGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGA                                                                                                                                      >  SRR19973066.2488761/1‑150 (MQ=60)

                 AGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGA                                                                                                                                      <  SRR19973066.3135289/150‑1 (MQ=60)

                  GCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGAT                                                                                                                                     <  SRR19973066.50508/150‑1 (MQ=60)

                   CAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATC                                                                                                                                    <  SRR19973066.1972914/150‑1 (MQ=60)

                   CAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATC                                                                                                                                    >  SRR19973066.3408752/1‑150 (MQ=60)

                    AGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCG                                                                                                                                   <  SRR19973066.3201247/150‑1 (MQ=60)

                    AGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCG                                                                                                                                   >  SRR19973066.3294081/1‑150 (MQ=60)

                        AGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAA                                                                                                                               <  SRR19973066.216577/150‑1 (MQ=60)

                        AGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAA                                                                                                                               <  SRR19973066.216153/150‑1 (MQ=60)

                        AGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAA                                                                                                                               >  SRR19973066.3336875/1‑150 (MQ=60)

                        AGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAA                                                                                                                               <  SRR19973066.216063/150‑1 (MQ=60)

                        AGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAA                                                                                                                               <  SRR19973066.617181/150‑1 (MQ=60)

                         GTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGGCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAA                                                                                                                              >  SRR19973066.3356736/1‑150 (MQ=60)

                         GTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGGCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAA                                                                                                                              >  SRR19973066.3356229/1‑150 (MQ=60)

                           CAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATG                                                                                                                            >  SRR19973066.212915/1‑150 (MQ=60)

                           CAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATG                                                                                                                            >  SRR19973066.1864781/1‑150 (MQ=60)

                           CAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATG                                                                                                                            >  SRR19973066.1865764/1‑150 (MQ=60)

                            AGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGC                                                                                                                           >  SRR19973066.3056746/1‑150 (MQ=60)

                            AGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGC                                                                                                                           >  SRR19973066.1606253/1‑150 (MQ=60)

                             GCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCC                                                                                                                          >  SRR19973066.423176/1‑150 (MQ=60)

                               TTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAG                                                                                                                        >  SRR19973066.940350/1‑150 (MQ=60)

                                  CNGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAG                                                                                                                     >  SRR19973066.979114/1‑150 (MQ=60)

                                  CAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAG                                                                                                                     <  SRR19973066.3225609/150‑1 (MQ=60)

                                  CAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAG                                                                                                                     <  SRR19973066.918092/150‑1 (MQ=60)

                                   AGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGG                                                                                                                    <  SRR19973066.3471939/150‑1 (MQ=60)

                                    GTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGC                                                                                                                   >  SRR19973066.1630985/1‑150 (MQ=60)

                                     TTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCG                                                                                                                  <  SRR19973066.534244/150‑1 (MQ=60)

                                     TTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCG                                                                                                                  <  SRR19973066.534826/150‑1 (MQ=60)

                                        TTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATC                                                                                                               <  SRR19973066.3242936/150‑1 (MQ=60)

                                         TTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCG                                                                                                              >  SRR19973066.503537/1‑150 (MQ=60)

                                         TTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCG                                                                                                              >  SRR19973066.2308330/1‑150 (MQ=60)

                                            CCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGG                                                                                                           >  SRR19973066.523106/1‑150 (MQ=60)

                                            CCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGG                                                                                                           >  SRR19973066.523098/1‑150 (MQ=60)

                                             CAGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGG                                                                                                          <  SRR19973066.2574791/150‑1 (MQ=60)

                                               GACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTG                                                                                                        >  SRR19973066.3480168/1‑150 (MQ=60)

                                               GACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTG                                                                                                        >  SRR19973066.3480437/1‑150 (MQ=60)

                                               GACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTG                                                                                                        <  SRR19973066.497871/150‑1 (MQ=60)

                                                 CTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTT                                                                                                      <  SRR19973066.539918/150‑1 (MQ=60)

                                                 CTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTT                                                                                                      >  SRR19973066.2991792/1‑150 (MQ=60)

                                                    CAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGAT                                                                                                   >  SRR19973066.3128716/1‑150 (MQ=60)

                                                    CAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGAT                                                                                                   >  SRR19973066.2517425/1‑150 (MQ=60)

                                                     AGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATC                                                                                                  <  SRR19973066.244880/150‑1 (MQ=60)

                                                       ATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGagatcggaagagcacacgtctgaa                                                                                                >  SRR19973066.2846694/1‑126 (MQ=60)

                                                       ATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAG                                                                                                >  SRR19973066.3225177/1‑150 (MQ=60)

                                                       ATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAG                                                                                                >  SRR19973066.3224596/1‑150 (MQ=60)

                                                       ATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAG                                                                                                <  SRR19973066.971742/150‑1 (MQ=60)

                                                          CATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATC                                                                                             <  SRR19973066.2565254/150‑1 (MQ=60)

                                                           ATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCC                                                                                            <  SRR19973066.1828371/150‑1 (MQ=60)

                                                           ATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCA                                                                                            >  SRR19973066.1669599/1‑150 (MQ=60)

                                                            TGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCA                                                                                           >  SRR19973066.493025/1‑150 (MQ=60)

                                                            TGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCA                                                                                           <  SRR19973066.988241/150‑1 (MQ=60)

                                                              ATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACT                                                                                         >  SRR19973066.676977/1‑150 (MQ=60)

                                                              ATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACT                                                                                         <  SRR19973066.1408085/150‑1 (MQ=60)

                                                                   ACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTAC                                                                                    >  SRR19973066.1747889/1‑150 (MQ=60)

                                                                   ACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTAC                                                                                    >  SRR19973066.1747764/1‑150 (MQ=60)

                                                                      ATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATC                                                                                 >  SRR19973066.3577902/1‑150 (MQ=60)

                                                                        CGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCA                                                                               >  SRR19973066.1662769/1‑150 (MQ=60)

                                                                         GTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAT                                                                              >  SRR19973066.242268/1‑150 (MQ=60)

                                                                         GTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAT                                                                              >  SRR19973066.2265009/1‑150 (MQ=60)

                                                                            ACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACC                                                                           <  SRR19973066.2596007/150‑1 (MQ=60)

                                                                            ACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACC                                                                           <  SRR19973066.2596002/150‑1 (MQ=60)

                                                                            ACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACC                                                                           <  SRR19973066.1841485/150‑1 (MQ=60)

                                                                              AGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGC                                                                         >  SRR19973066.1332887/1‑150 (MQ=60)

                                                                               GCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCG                                                                        <  SRR19973066.765797/150‑1 (MQ=60)

                                                                                   TCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAG                                                                    <  SRR19973066.862393/150‑1 (MQ=60)

                                                                                     GATCTTCTCGTACTCTTCGTTGTGACTAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAT                                                                  >  SRR19973066.1856912/1‑150 (MQ=60)

                                                                                      ATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGAGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATC                                                                 >  SRR19973066.2241124/1‑150 (MQ=60)

                                                                                       TCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCG                                                                <  SRR19973066.2259021/150‑1 (MQ=60)

                                                                                       TCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCG                                                                <  SRR19973066.2046978/150‑1 (MQ=60)

                                                                                        CTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGA                                                               >  SRR19973066.2578532/1‑150 (MQ=60)

                                                                                          TCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACG                                                             >  SRR19973066.3525523/1‑150 (MQ=60)

                                                                                          TCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACG                                                             >  SRR19973066.3526631/1‑150 (MQ=60)

                                                                                             CGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCAT                                                          <  SRR19973066.3091645/150‑1 (MQ=60)

                                                                                              GTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATCCCGCGGAAGATCGACGCATA                                                         <  SRR19973066.901119/150‑1 (MQ=60)

                                                                                                ACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATT                                                       >  SRR19973066.172300/1‑150 (MQ=60)

                                                                                                  TCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCA                                                     >  SRR19973066.2348024/1‑150 (MQ=60)

                                                                                                   CTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCAC                                                    >  SRR19973066.1592687/1‑150 (MQ=60)

                                                                                                         TGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGA                                              >  SRR19973066.1593763/1‑150 (MQ=60)

                                                                                                         TGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGA                                              <  SRR19973066.93885/150‑1 (MQ=60)

                                                                                                         TGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGA                                              <  SRR19973066.94048/150‑1 (MQ=60)

                                                                                                         TGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGA                                              >  SRR19973066.392591/1‑150 (MQ=60)

                                                                                                              CGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGTGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACT                                         <  SRR19973066.1418491/150‑1 (MQ=60)

                                                                                                               GAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTC                                        >  SRR19973066.2904713/1‑150 (MQ=60)

                                                                                                                AATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCC                                       >  SRR19973066.3380528/1‑150 (MQ=60)

                                                                                                                 ATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCA                                      <  SRR19973066.2062092/150‑1 (MQ=60)

                                                                                                                  TCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAG                                     >  SRR19973066.3295196/1‑150 (MQ=60)

                                                                                                                  TCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAG                                     >  SRR19973066.3295350/1‑150 (MQ=60)

                                                                                                                  TCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAG                                     >  SRR19973066.3295387/1‑150 (MQ=60)

                                                                                                                  TCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAG                                     >  SRR19973066.1190786/1‑150 (MQ=60)

                                                                                                                     CTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAC                                  >  SRR19973066.308743/1‑150 (MQ=60)

                                                                                                                        CGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCAT                               >  SRR19973066.1296194/1‑150 (MQ=60)

                                                                                                                         GCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATA                              >  SRR19973066.2804441/1‑150 (MQ=60)

                                                                                                                              TGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAA                         <  SRR19973066.1095028/150‑1 (MQ=60)

                                                                                                                              TGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAA                         <  SRR19973066.3122299/150‑1 (MQ=60)

                                                                                                                               GTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAG                        <  SRR19973066.1703488/150‑1 (MQ=60)

                                                                                                                                  CCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAC                     <  SRR19973066.2497702/150‑1 (MQ=60)

                                                                                                                                  CCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAC                     <  SRR19973066.2499422/150‑1 (MQ=60)

                                                                                                                                    AGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACAT                   <  SRR19973066.3097638/150‑1 (MQ=60)

                                                                                                                                     GAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATG                  <  SRR19973066.3520493/150‑1 (MQ=60)

                                                                                                                                     GAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATG                  <  SRR19973066.1711675/150‑1 (MQ=60)

                                                                                                                                     GAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATG                  <  SRR19973066.1747007/150‑1 (MQ=60)

                                                                                                                                     GAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATG                  <  SRR19973066.3589221/150‑1 (MQ=60)

                                                                                                                                     GAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTAAAGACAGACCGCGGAATCTAGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATG                  <  SRR19973066.3520882/150‑1 (MQ=60)

                                                                                                                                      AAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGG                 >  SRR19973066.1582917/1‑150 (MQ=60)

                                                                                                                                       AGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGC                >  SRR19973066.1030892/1‑150 (MQ=60)

                                                                                                                                        GTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCG               <  SRR19973066.112503/150‑1 (MQ=60)

                                                                                                                                         TGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGC              >  SRR19973066.3225481/1‑150 (MQ=60)

                                                                                                                                         TGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGC              >  SRR19973066.1172350/1‑150 (MQ=60)

                                                                                                                                         TGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGC              >  SRR19973066.1867173/1‑150 (MQ=60)

                                                                                                                                          GATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCG             >  SRR19973066.2261633/1‑150 (MQ=60)

                                                                                                                                            TAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGT           >  SRR19973066.3027421/1‑150 (MQ=60)

                                                                                                                                            TAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGT           >  SRR19973066.1239981/1‑150 (MQ=60)

                                                                                                                                            TAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGT           >  SRR19973066.2945709/1‑150 (MQ=60)

                                                                                                                                             AGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTT          >  SRR19973066.702762/1‑150 (MQ=60)

                                                                                                                                               GAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCG        <  SRR19973066.2404661/150‑1 (MQ=60)

                                                                                                                                                 GAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG      >  SRR19973066.666225/1‑150 (MQ=60)

                                                                                                                                                 GAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG      >  SRR19973066.1401207/1‑150 (MQ=60)

                                                                                                                                                 GAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG      >  SRR19973066.1317989/1‑150 (MQ=60)

                                                                                                                                                 GAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG      >  SRR19973066.3105633/1‑150 (MQ=60)

                                                                                                                                                 GAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG      >  SRR19973066.3105619/1‑150 (MQ=60)

                                                                                                                                                    ATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTG   <  SRR19973066.3402725/150‑1 (MQ=60)

                                                                                                                                                    ATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTG   <  SRR19973066.1624559/150‑1 (MQ=60)

                                                                                                                                                     TAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGC  >  SRR19973066.2146190/1‑150 (MQ=60)

TGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGC  >  CP009273/4149905‑4150203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`