Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,150,054 G→T 100% S222Y (TCT→TAT)  sthA ← pyridine nucleotide transhydrogenase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,150,0540GT99.5% 686.4 / ‑4.3 208S222Y (TCT→TAT) sthApyridine nucleotide transhydrogenase, soluble
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (111/96);  total (112/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG  >  CP009273/4149909‑4150199
                                                                                                                                                 |                                                                                                                                                 
aCCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATaa                                                                                                                                                 <  2:316501/148‑1 (MQ=255)
aCCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATaa                                                                                                                                                 <  1:2921934/148‑1 (MQ=255)
   gTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCt                                                                                                                                              >  2:3211307/1‑148 (MQ=255)
     tGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGa                                                                                                                                            >  2:1624654/1‑148 (MQ=255)
     tGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGa                                                                                                                                            >  1:332502/1‑148 (MQ=255)
      ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGAc                                                                                                                                           <  2:1397250/148‑1 (MQ=255)
      ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGAc                                                                                                                                           >  2:1210503/1‑148 (MQ=255)
        cATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAt                                                                                                                                         <  1:2716119/148‑1 (MQ=255)
            gagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATctct                                                                                                                                     <  1:158779/148‑1 (MQ=255)
             agCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCtt                                                                                                                                    >  2:652744/1‑148 (MQ=255)
              gcaggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTg                                                                                                                                   >  2:816535/1‑148 (MQ=255)
              gcaggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTg                                                                                                                                   <  1:2062030/148‑1 (MQ=255)
               caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                  >  2:1746794/1‑148 (MQ=255)
               caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                  <  1:675921/148‑1 (MQ=255)
                aggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGAt                                                                                                                                 <  2:4242/148‑1 (MQ=255)
                  gcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCCTCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCg                                                                                                                               <  2:2159734/148‑1 (MQ=255)
                  gcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCg                                                                                                                               >  1:2264249/1‑148 (MQ=255)
                      tCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGaa                                                                                                                           >  2:408675/1‑148 (MQ=255)
                       cAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGaaa                                                                                                                          <  2:3125419/148‑1 (MQ=255)
                         gCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATg                                                                                                                        >  2:2442263/1‑148 (MQ=255)
                           tttCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGcc                                                                                                                      <  2:2387300/148‑1 (MQ=255)
                           tttCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGcc                                                                                                                      <  1:2911353/148‑1 (MQ=255)
                              cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagc                                                                                                                   >  1:3383634/1‑148 (MQ=255)
                              cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagc                                                                                                                   <  2:3504891/148‑1 (MQ=255)
                              cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagc                                                                                                                   >  1:46260/1‑148 (MQ=255)
                                 ttttttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAgg                                                                                                                >  1:2949096/1‑148 (MQ=255)
                                    tttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa                                                                                                             <  1:1772096/148‑1 (MQ=255)
                                    tttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa                                                                                                             <  1:2038278/148‑1 (MQ=255)
                                     ttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGAt                                                                                                            <  2:356327/148‑1 (MQ=255)
                                       aCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcg                                                                                                          >  2:3122008/1‑148 (MQ=255)
                                       aCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcg                                                                                                          >  2:167932/1‑148 (MQ=255)
                                          cGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg                                                                                                       <  1:1278877/148‑1 (MQ=255)
                                            acgtCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgt                                                                                                     <  2:2043654/145‑1 (MQ=255)
                                             cTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg                                                                                                    <  2:197088/148‑1 (MQ=255)
                                             cTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg                                                                                                    >  1:2969611/1‑148 (MQ=255)
                                              ttCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtgt                                                                                                   >  1:646697/1‑148 (MQ=255)
                                              ttCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtgt                                                                                                   >  1:3487094/1‑148 (MQ=255)
                                               tCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGtt                                                                                                  >  1:1998984/1‑148 (MQ=255)
                                                  gATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGAt                                                                                               >  1:2025892/1‑148 (MQ=255)
                                                   aTGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATc                                                                                              >  2:2130815/1‑148 (MQ=255)
                                                     gCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGACGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAg                                                                                            >  2:708396/1‑148 (MQ=255)
                                                     gCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAg                                                                                            <  1:1800949/148‑1 (MQ=255)
                                                       aTGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCCGGCGATCGCGGGTGTTGATCAGAt                                                                                          <  1:2419958/148‑1 (MQ=255)
                                                            cacaCCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                     <  1:2442263/148‑1 (MQ=255)
                                                             acacCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACtt                                                                                    <  2:3467122/148‑1 (MQ=255)
                                                              cacCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt                                                                                   >  1:1481660/1‑148 (MQ=255)
                                                                  aTCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACa                                                                               <  2:870346/148‑1 (MQ=255)
                                                                     gTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATcc                                                                            <  2:592290/148‑1 (MQ=255)
                                                                      tCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCa                                                                           <  2:313476/148‑1 (MQ=255)
                                                                       cacaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAt                                                                          >  2:2912095/1‑148 (MQ=255)
                                                                       cacaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAt                                                                          >  2:274545/1‑148 (MQ=255)
                                                                                 gATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAg                                                                <  2:2867297/148‑1 (MQ=255)
                                                                                  aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGa                                                               <  2:2177864/148‑1 (MQ=255)
                                                                                   tcttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAt                                                              >  2:2640089/1‑148 (MQ=255)
                                                                                    cttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATc                                                             >  1:156474/1‑148 (MQ=255)
                                                                                         cGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGc                                                        >  2:1175595/1‑148 (MQ=255)
                                                                                           tACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat                                                      <  2:3059028/148‑1 (MQ=255)
                                                                                            aCTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCata                                                     >  1:313137/1‑148 (MQ=255)
                                                                                            aCTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCata                                                     >  1:2734189/1‑148 (MQ=255)
                                                                                              tctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATAtt                                                   >  1:1966729/1‑148 (MQ=255)
                                                                                              tctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATAtt                                                   >  2:3257227/1‑148 (MQ=255)
                                                                                               ctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTc                                                  >  1:3454677/1‑148 (MQ=255)
                                                                                               ctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTc                                                  <  1:312284/148‑1 (MQ=255)
                                                                                                ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTca                                                 <  2:332502/148‑1 (MQ=255)
                                                                                                ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTca                                                 >  1:131834/1‑148 (MQ=255)
                                                                                                ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTca                                                 >  1:132853/1‑148 (MQ=255)
                                                                                                 tCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcac                                                >  1:709320/1‑148 (MQ=255)
                                                                                                  cGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcaca                                               >  2:2778009/1‑148 (MQ=255)
                                                                                                  cGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcaca                                               <  1:950466/148‑1 (MQ=255)
                                                                                                    ttGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc                                             >  2:2085209/1‑148 (MQ=255)
                                                                                                     ttttaCGTATCAATACTCCACTGTTCCAGATGTGATTGGAGATATAATCTGACATCTCTTGATCGATAAATGCCAGCTGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGcc                                            <  1:3440094/144‑1 (MQ=255)
                                                                                                     tgtgACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGcc                                            <  1:915421/148‑1 (MQ=255)
                                                                                                        gACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGAt                                         >  2:1121153/1‑148 (MQ=255)
                                                                                                         aCGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATc                                        >  1:1250158/1‑148 (MQ=255)
                                                                                                           gAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCAc                                      <  1:2561403/148‑1 (MQ=255)
                                                                                                            aaTCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACt                                     <  2:129898/148‑1 (MQ=255)
                                                                                                              tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAg                                              <  2:1864984/137‑1 (MQ=255)
                                                                                                              tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAg                                              >  1:1864984/1‑137 (MQ=255)
                                                                                                              tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTcc                                   >  1:3235702/1‑148 (MQ=255)
                                                                                                              tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTcc                                   >  1:2994657/1‑148 (MQ=255)
                                                                                                                aCTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAg                                 >  2:3400272/1‑148 (MQ=255)
                                                                                                                 cTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGc                                >  1:435813/1‑148 (MQ=255)
                                                                                                                   aCGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                              >  2:787390/1‑148 (MQ=255)
                                                                                                                   aCGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                              >  1:1984423/1‑148 (MQ=255)
                                                                                                                    cGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAcc                             >  1:86700/1‑148 (MQ=255)
                                                                                                                    cGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAcc                             <  1:2455887/148‑1 (MQ=255)
                                                                                                                      ccACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCAt                           <  1:434128/148‑1 (MQ=255)
                                                                                                                           gTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATa                      <  1:1423870/148‑1 (MQ=255)
                                                                                                                             tCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAg                    >  2:769913/1‑148 (MQ=255)
                                                                                                                                 gAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACa                <  1:167932/148‑1 (MQ=255)
                                                                                                                                   aGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATg              <  2:1250158/148‑1 (MQ=255)
                                                                                                                                   aGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATg              <  1:2337049/148‑1 (MQ=255)
                                                                                                                                       aTAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGgcgc          >  2:2698843/1‑148 (MQ=255)
                                                                                                                                       aTAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGgcgc          >  1:593571/1‑148 (MQ=255)
                                                                                                                                         aGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCgg        <  1:1210503/148‑1 (MQ=255)
                                                                                                                                            agagataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTc     >  1:3220282/1‑148 (MQ=255)
                                                                                                                                            agagataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTc     >  1:1570258/1‑148 (MQ=255)
                                                                                                                                             gagataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTCCAGCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATACGTACCGGGCGCGGTTCg    <  2:2282971/148‑1 (MQ=255)
                                                                                                                                              agataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTctt   >  1:1040755/1‑146 (MQ=255)
                                                                                                                                               gagaATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCgtg  <  2:501667/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG  >  CP009273/4149909‑4150199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGC  >  CP009273/4149905‑4150203
                                                                                                                                                     |                                                                                                                                                     
TGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAT                                                                                                                                                       <  SRR19973065.585655/150‑1 (MQ=60)
TGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAT                                                                                                                                                       <  SRR19973065.2359525/150‑1 (MQ=60)
  CGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAA                                                                                                                                                     <  SRR19973065.1816225/150‑1 (MQ=60)
   GACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAAT                                                                                                                                                    <  SRR19973065.1377784/150‑1 (MQ=60)
   GACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAAT                                                                                                                                                    <  SRR19973065.1287622/150‑1 (MQ=60)
   GACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAAT                                                                                                                                                    <  SRR19973065.1375566/150‑1 (MQ=60)
    ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATC                                                                                                                                                   <  SRR19973065.2939694/150‑1 (MQ=60)
    ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATC                                                                                                                                                   <  SRR19973065.2940188/150‑1 (MQ=60)
     CCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCT                                                                                                                                                  >  SRR19973065.2297665/1‑150 (MQ=60)
       GTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGA                                                                                                                                                >  SRR19973065.334828/1‑150 (MQ=60)
       GTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGA                                                                                                                                                >  SRR19973065.334859/1‑150 (MQ=60)
            CATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCTTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCT                                                                                                                                           >  SRR19973065.3247438/1‑150 (MQ=60)
            CATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCT                                                                                                                                           <  SRR19973065.2898628/150‑1 (MQ=60)
            CATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCT                                                                                                                                           <  SRR19973065.2733015/150‑1 (MQ=60)
            CATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCT                                                                                                                                           <  SRR19973065.2732672/150‑1 (MQ=60)
             ATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTC                                                                                                                                          >  SRR19973065.667049/1‑150 (MQ=60)
                GAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTG                                                                                                                                       <  SRR19973065.160093/150‑1 (MQ=60)
                  GCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGAT                                                                                                                                     <  SRR19973065.2075181/150‑1 (MQ=60)
                  GCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGAT                                                                                                                                     <  SRR19973065.400591/150‑1 (MQ=60)
                   CAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATC                                                                                                                                    <  SRR19973065.2451384/150‑1 (MQ=60)
                   CAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATC                                                                                                                                    <  SRR19973065.680424/150‑1 (MQ=60)
                    AGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCG                                                                                                                                   >  SRR19973065.2278563/1‑150 (MQ=60)
                               TTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAG                                                                                                                        <  SRR19973065.2929072/150‑1 (MQ=60)
                               TTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAG                                                                                                                        >  SRR19973065.312897/1‑150 (MQ=60)
                                TTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGC                                                                                                                       >  SRR19973065.3404324/1‑150 (MQ=60)
                                TTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGC                                                                                                                       >  SRR19973065.46799/1‑150 (MQ=60)
                                  CAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAG                                                                                                                     >  SRR19973065.399780/1‑150 (MQ=60)
                                  CAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAG                                                                                                                     <  SRR19973065.1261542/150‑1 (MQ=60)
                                   AGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGG                                                                                                                    >  SRR19973065.2967052/1‑150 (MQ=60)
                                      TTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACACTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAAAGGAGAGATAATCTGACATCTCTTGAACGAGAAATGCCAGCAGGCGA                                                                                                                 >  SRR19973065.1300649/1‑150 (MQ=60)
                                        TTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATC                                                                                                               <  SRR19973065.1781713/150‑1 (MQ=60)
                                        TTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATC                                                                                                               <  SRR19973065.1783696/150‑1 (MQ=60)
                                        TTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATC                                                                                                               <  SRR19973065.2051338/150‑1 (MQ=60)
                                              CGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGT                                                                                                         <  SRR19973065.1287319/150‑1 (MQ=60)
                                               GACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTG                                                                                                        >  SRR19973065.2987644/1‑150 (MQ=60)
                                               GACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTG                                                                                                        >  SRR19973065.2987772/1‑150 (MQ=60)
                                                ACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGT                                                                                                       <  SRR19973065.1178202/150‑1 (MQ=60)
                                                ACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGT                                                                                                       <  SRR19973065.197756/150‑1 (MQ=60)
                                                ACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGT                                                                                                       >  SRR19973065.3508302/1‑150 (MQ=60)
                                                ACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGT                                                                                                       >  SRR19973065.1799117/1‑150 (MQ=60)
                                                ACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGT                                                                                                       >  SRR19973065.650949/1‑150 (MQ=60)
                                                 CTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTT                                                                                                      >  SRR19973065.2011925/1‑150 (MQ=60)
                                                    CAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGAT                                                                                                   >  SRR19973065.2038914/1‑150 (MQ=60)
                                                     AGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATC                                                                                                  >  SRR19973065.2237437/1‑150 (MQ=60)
                                                     AGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATC                                                                                                  >  SRR19973065.1999765/1‑150 (MQ=60)
                                                       ATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATACTCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAG                                                                                                >  SRR19973065.1585236/1‑150 (MQ=60)
                                                       ATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAG                                                                                                >  SRR19973065.31700/1‑150 (MQ=60)
                                                       ATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAG                                                                                                <  SRR19973065.247126/150‑1 (MQ=60)
                                                         GCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGAT                                                                                              <  SRR19973065.1812775/150‑1 (MQ=60)
                                                           ATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCCGGCGATCGCGGGTGTTGATCAGATCC                                                                                            <  SRR19973065.2435217/150‑1 (MQ=60)
                                                           ATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCC                                                                                            <  SRR19973065.2067883/150‑1 (MQ=60)
                                                             GATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCAC                                                                                          >  SRR19973065.2933322/1‑150 (MQ=60)
                                                                CACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTT                                                                                       >  SRR19973065.1491123/1‑150 (MQ=60)
                                                                CACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTT                                                                                       <  SRR19973065.2457657/150‑1 (MQ=60)
                                                                  CACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTA                                                                                     <  SRR19973065.1383323/150‑1 (MQ=60)
                                                                       TCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCC                                                                                <  SRR19973065.204201/150‑1 (MQ=60)
                                                                       TCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCC                                                                                <  SRR19973065.1831209/150‑1 (MQ=60)
                                                                        CGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCA                                                                               <  SRR19973065.3176632/150‑1 (MQ=60)
                                                                            ACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACC                                                                           <  SRR19973065.1981245/150‑1 (MQ=60)
                                                                              AGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGC                                                                         >  SRR19973065.463579/1‑150 (MQ=60)
                                                                                     GATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAT                                                                  >  SRR19973065.2038094/1‑150 (MQ=60)
                                                                                      ATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATC                                                                 >  SRR19973065.157772/1‑150 (MQ=60)
                                                                                      ATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATC                                                                 >  SRR19973065.158008/1‑150 (MQ=60)
                                                                                         TTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGAC                                                              <  SRR19973065.3230622/150‑1 (MQ=60)
                                                                                             CGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCAT                                                          <  SRR19973065.1574041/150‑1 (MQ=60)
                                                                                              GTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATA                                                         >  SRR19973065.315252/1‑150 (MQ=60)
                                                                                              GTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATA                                                         >  SRR19973065.2751123/1‑150 (MQ=60)
                                                                                              GTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATA                                                         >  SRR19973065.2751197/1‑150 (MQ=60)
                                                                                                ACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATT                                                       >  SRR19973065.1979564/1‑150 (MQ=60)
                                                                                                 CTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTC                                                      >  SRR19973065.3475733/1‑150 (MQ=60)
                                                                                                  TCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCA                                                     >  SRR19973065.133994/1‑150 (MQ=60)
                                                                                                  TCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCA                                                     >  SRR19973065.132966/1‑150 (MQ=60)
                                                                                                   CTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCAC                                                    >  SRR19973065.714060/1‑150 (MQ=60)
                                                                                                   CTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCAC                                                    <  SRR19973065.314392/150‑1 (MQ=60)
                                                                                                   CTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCAC                                                    >  SRR19973065.636552/1‑150 (MQ=60)
                                                                                                     TCTTTTTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATATTCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCTCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAG                                                  <  SRR19973065.1239234/150‑1 (MQ=60)
                                                                                                      CGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGC                                                 <  SRR19973065.956745/150‑1 (MQ=60)
                                                                                                         ttttacgtatcaatactCCACTGTTCCAGATGTGATTGGAGATATAATCTGACATCTCTTGATCGATAAATGCCAGCTGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGA                                              <  SRR19973065.3461076/133‑1 (MQ=60)
                                                                                                         TGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGA                                              <  SRR19973065.921396/150‑1 (MQ=60)
                                                                                                           TGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATC                                            >  SRR19973065.1258391/1‑150 (MQ=60)
                                                                                                              CGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACT                                         >  SRR19973065.746460/1‑150 (MQ=60)
                                                                                                              CGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACT                                         >  SRR19973065.994488/1‑150 (MQ=60)
                                                                                                              CGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACT                                         >  SRR19973065.2757990/1‑150 (MQ=60)
                                                                                                               GAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTC                                        <  SRR19973065.2577417/150‑1 (MQ=60)
                                                                                                                AATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCagatcggaa                                       >  SRR19973065.1877257/1‑141 (MQ=60)
                                                                                                                AATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCC                                       >  SRR19973065.3010738/1‑150 (MQ=60)
                                                                                                                AATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCC                                       >  SRR19973065.3012997/1‑150 (MQ=60)
                                                                                                                AATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCC                                       <  SRR19973065.3430245/150‑1 (MQ=60)
                                                                                                                AATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCC                                       >  SRR19973065.3255176/1‑150 (MQ=60)
                                                                                                                AATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACAGCCATACCGCGGAAGATCGACGCATATTCtccgcgagatcggac                                       >  SRR19973065.1877325/1‑135 (MQ=60)
                                                                                                                   CACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGC                                    >  SRR19973065.438841/1‑150 (MQ=60)
                                                                                                                   CACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGC                                    >  SRR19973065.3482889/1‑150 (MQ=60)
                                                                                                                    ACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCA                                   >  SRR19973065.705569/1‑150 (MQ=60)
                                                                                                                     CTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAC                                  >  SRR19973065.1997307/1‑150 (MQ=60)
                                                                                                                      TACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACC                                 >  SRR19973065.87504/1‑150 (MQ=60)
                                                                                                                      TACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACC                                 >  SRR19973065.87543/1‑150 (MQ=60)
                                                                                                                      TACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTGTACATCCATGCCGCGGccggtcgacgcgtgggggcggcgggtggcgccggcccc                                 >  SRR19973065.1860900/1‑112 (MQ=60)
                                                                                                                        CGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCAT                               <  SRR19973065.2471365/150‑1 (MQ=60)
                                                                                                                          CCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAG                             <  SRR19973065.437199/150‑1 (MQ=60)
                                                                                                                          CCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAG                             <  SRR19973065.437145/150‑1 (MQ=60)
                                                                                                                               GTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAG                        <  SRR19973065.1433151/150‑1 (MQ=60)
                                                                                                                               GTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAG                        <  SRR19973065.48755/150‑1 (MQ=60)
                                                                                                                                   CAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACA                    <  SRR19973065.3339525/150‑1 (MQ=60)
                                                                                                                                   CAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACA                    >  SRR19973065.1909424/1‑150 (MQ=60)
                                                                                                                                   CAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACA                    <  SRR19973065.922159/150‑1 (MQ=60)
                                                                                                                                     TAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATG                  <  SRR19973065.169294/150‑1 (MQ=60)
                                                                                                                                     GAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCTCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAATATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATG                  <  SRR19973065.1508667/150‑1 (MQ=60)
                                                                                                                                       AGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATGGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGC                >  SRR19973065.2535998/1‑150 (MQ=60)
                                                                                                                                       AGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGC                <  SRR19973065.2352227/150‑1 (MQ=60)
                                                                                                                                       AGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGC                <  SRR19973065.2351640/150‑1 (MQ=60)
                                                                                                                                       AGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGC                <  SRR19973065.2351620/150‑1 (MQ=60)
                                                                                                                                         TGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGC              >  SRR19973065.597237/1‑150 (MQ=60)
                                                                                                                                            TAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGT           >  SRR19973065.1655419/1‑150 (MQ=60)
                                                                                                                                            TAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGT           >  SRR19973065.3470671/1‑150 (MQ=60)
                                                                                                                                            TAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGT           >  SRR19973065.1335125/1‑150 (MQ=60)
                                                                                                                                            TAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGT           >  SRR19973065.874592/1‑150 (MQ=60)
                                                                                                                                             AGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTT          <  SRR19973065.1218311/150‑1 (MQ=60)
                                                                                                                                              GGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTC         >  SRR19973065.1580701/1‑150 (MQ=60)
                                                                                                                                              GGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTC         >  SRR19973065.3239638/1‑150 (MQ=60)
                                                                                                                                              GGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTC         >  SRR19973065.1486197/1‑150 (MQ=60)
                                                                                                                                                AGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCTT       >  SRR19973065.1047643/1‑150 (MQ=60)
                                                                                                                                                     TAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGC  >  SRR19973065.3357260/1‑150 (MQ=60)
                                                                                                                                                     TAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGC  >  SRR19973065.3357365/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGC  >  CP009273/4149905‑4150203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑