Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,176,823 G→T 100% D516Y (GAC→TAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,176,8230GT98.9% 617.4 / ‑1.9 189D516Y (GAC→TAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (93/94);  total (95/94)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 189 total aligned reads displayed.

GTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAG  >  CP009273/4176679‑4176967
                                                                                                                                                |                                                                                                                                                
gTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACt                                                                                                                                               >  2:2889325/1‑148 (MQ=255)
 ttCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtg                                                                                                                                              >  1:751342/1‑148 (MQ=255)
 ttCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtg                                                                                                                                              <  2:1873062/148‑1 (MQ=255)
  tCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACtgt                                                                                                                                             >  1:1192084/1‑148 (MQ=255)
      gTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTa                                                                                                                                         <  1:1467093/148‑1 (MQ=255)
       tACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTaa                                                                                                                                        <  2:2710640/148‑1 (MQ=255)
             tGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCaa                                                                                                                                  <  1:944749/148‑1 (MQ=255)
             tGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCaa                                                                                                                                  <  1:2726353/148‑1 (MQ=255)
             tGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCaa                                                                                                                                  <  2:2517370/148‑1 (MQ=255)
              gACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCaaa                                                                                                                                 >  1:2191516/1‑148 (MQ=255)
               aCGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAg                                                                                                                                >  2:1761352/1‑148 (MQ=255)
               aCGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAg                                                                                                                                <  2:2448231/148‑1 (MQ=255)
                    ggAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGaa                                                                                                                           >  1:1976831/1‑148 (MQ=255)
                     gAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAg                                                                                                                          >  1:2040080/1‑148 (MQ=255)
                        gCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCa                                                                                                                       >  2:1651470/1‑148 (MQ=255)
                          ccAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATg                                                                                                                     <  2:3217214/148‑1 (MQ=255)
                           cAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATgg                                                                                                                    >  2:699728/1‑148 (MQ=255)
                           cAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATgg                                                                                                                    <  2:2369050/148‑1 (MQ=255)
                                 gAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGa                                                                                                              >  2:1055007/1‑148 (MQ=255)
                                  aaGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                                                                                                             >  2:3401025/1‑148 (MQ=255)
                                  aaGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                                                                                                             <  2:1506296/148‑1 (MQ=255)
                                    gcgcGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTg                                                                                                           >  1:1650854/1‑148 (MQ=255)
                                       cgTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGcc                                                                                                        >  2:2023198/1‑148 (MQ=255)
                                              tgatgaTGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaaga                                                                                                 <  1:731220/148‑1 (MQ=255)
                                              tgatgaTGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaaga                                                                                                 >  2:153222/1‑148 (MQ=255)
                                               gatgatGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaa                                                                                                >  2:2867613/1‑148 (MQ=255)
                                                atgatgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaag                                                                                               >  2:2839691/1‑148 (MQ=255)
                                                atgatgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaag                                                                                               >  2:148160/1‑148 (MQ=255)
                                                atgatgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaag                                                                                               >  2:3436908/1‑148 (MQ=255)
                                                atgatgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaag                                                                                               >  2:2839672/1‑148 (MQ=255)
                                                atgatgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaag                                                                                               >  2:2839658/1‑148 (MQ=255)
                                                  gatgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCa                                                                                             <  2:985159/148‑1 (MQ=255)
                                                    tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGa                                                                                           <  1:2070023/148‑1 (MQ=255)
                                                    tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGa                                                                                           >  2:1592590/1‑148 (MQ=255)
                                                    tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGa                                                                                           <  1:492512/148‑1 (MQ=255)
                                                    tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGa                                                                                           <  1:159169/148‑1 (MQ=255)
                                                     gTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGaa                                                                                          >  2:1350243/1‑148 (MQ=255)
                                                      tCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAc                                                                                         >  1:1926969/1‑148 (MQ=255)
                                                      tCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAc                                                                                         >  1:1364964/1‑148 (MQ=255)
                                                         aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTc                                                                                      >  2:754129/1‑148 (MQ=255)
                                                         aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTc                                                                                      >  2:3242280/1‑148 (MQ=255)
                                                          ccAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCt                                                                                     >  1:1845072/1‑148 (MQ=255)
                                                           caacaaCATCCTGTCCCCGGCGAACGGCGAACCACTCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTg                                                                                    <  2:625588/148‑1 (MQ=255)
                                                            aacaacATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGt                                                                                   <  2:2783143/148‑1 (MQ=255)
                                                              caacaTCCTGTCCCCGGCGAACGGCGAACCACTCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTAt                                                                                 <  2:2277254/148‑1 (MQ=255)
                                                              caacaTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTAt                                                                                 <  1:1393683/148‑1 (MQ=255)
                                                                acaTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCg                                                                               <  1:838481/148‑1 (MQ=255)
                                                                        tCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtc                                                                       <  2:954034/148‑1 (MQ=255)
                                                                              gcgaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCtt                                                                 >  1:833734/1‑148 (MQ=255)
                                                                              gcgaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCtt                                                                 <  2:2340033/148‑1 (MQ=255)
                                                                               cgaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtc                                                                >  1:2328611/1‑148 (MQ=255)
                                                                                gatcggcgaacCAATCATCGGTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtct                                                               <  2:273023/145‑1 (MQ=255)
                                                                                gaacggcgaccCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtct                                                               <  1:2510827/148‑1 (MQ=255)
                                                                                gaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtct                                                               <  1:872156/148‑1 (MQ=255)
                                                                                      cgaacCTATCATCGTTCCTTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCAGAAAGAAGCAGAACGTATTTATCGCTCTGGTCTGGCTTCTCTGCAt                                                         <  2:2446012/148‑1 (MQ=255)
                                                                                          ccAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcgc                                                     >  2:766800/1‑148 (MQ=255)
                                                                                           cAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcgcg                                                    >  1:384019/1‑148 (MQ=255)
                                                                                              tcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGt                                                 <  1:1766271/148‑1 (MQ=255)
                                                                                                 tcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTACCGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTaa                                              <  1:2158039/148‑1 (MQ=255)
                                                                                                 tcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTaa                                              >  2:3562291/1‑148 (MQ=255)
                                                                                                 tcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTaa                                              >  2:571719/1‑148 (MQ=255)
                                                                                                 tcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTaa                                              <  1:361713/148‑1 (MQ=255)
                                                                                                 tcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTaa                                              <  1:111139/148‑1 (MQ=255)
                                                                                                 tcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTaa                                              <  1:1913211/148‑1 (MQ=255)
                                                                                                   gTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAg                                            <  1:2399207/148‑1 (MQ=255)
                                                                                                    ttCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGt                                           <  1:3327924/148‑1 (MQ=255)
                                                                                                    ttCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGt                                           <  1:299721/148‑1 (MQ=255)
                                                                                                     tCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                          >  2:3063647/1‑148 (MQ=255)
                                                                                                        gTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGt                                       <  1:3316081/148‑1 (MQ=255)
                                                                                                        gTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGt                                       >  1:2467886/1‑148 (MQ=255)
                                                                                                           tcAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATc                                    <  2:646369/148‑1 (MQ=255)
                                                                                                            cAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCa                                   >  1:3258388/1‑148 (MQ=255)
                                                                                                            cAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCa                                   >  2:1829194/1‑148 (MQ=255)
                                                                                                            cAGGACGTTGTACTGGGTATGTACTACATGACCCGTTACTGTGTTAACGCCATAGTCGTAGTCATGGTGCTGACTGGCCCGATTGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCa                                   <  1:1703369/148‑1 (MQ=255)
                                                                                                             aGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCAc                                  <  1:530700/148‑1 (MQ=255)
                                                                                                               gACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCg                                >  1:420023/1‑148 (MQ=255)
                                                                                                                  gTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGt                             <  2:3453816/148‑1 (MQ=255)
                                                                                                                  gTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGt                             <  1:1290881/148‑1 (MQ=255)
                                                                                                                  gTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGt                             <  1:3148183/148‑1 (MQ=255)
                                                                                                                   ttGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCTTATCACCGAGTa                            >  1:274445/1‑148 (MQ=255)
                                                                                                                   ttGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTa                            >  1:1313762/1‑148 (MQ=255)
                                                                                                                    tGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTAt                           <  1:2285719/148‑1 (MQ=255)
                                                                                                                        cTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaaa                       >  2:2296199/1‑148 (MQ=255)
                                                                                                                          gggTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaaaaa                     <  2:2040080/148‑1 (MQ=255)
                                                                                                                            gTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGa                   >  2:2098034/1‑148 (MQ=255)
                                                                                                                               tGTACTACATGACGCGTTACTGTGTTAACGCCAAAGTCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGc                <  2:3301875/148‑1 (MQ=255)
                                                                                                                               tGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGc                <  1:290726/148‑1 (MQ=255)
                                                                                                                                   ctacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAAc            >  2:2088005/1‑148 (MQ=255)
                                                                                                                                     acATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACgg          >  2:67674/1‑148 (MQ=255)
                                                                                                                                     acATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACgg          >  2:67791/1‑148 (MQ=255)
                                                                                                                                      cATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGt         <  2:815227/148‑1 (MQ=255)
                                                                                                                                       aTGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTg        <  1:2370997/148‑1 (MQ=255)
                                                                                                                                        tGAACCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGa       >  2:2796190/1‑148 (MQ=255)
                                                                                                                                         gACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGaa      <  2:2989355/148‑1 (MQ=255)
                                                                                                                                          aCCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTGAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAt     >  1:1736636/1‑148 (MQ=255)
                                                                                                                                           cccGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAtt    <  1:699728/148‑1 (MQ=255)
                                                                                                                                            ccGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATta   <  2:3265560/148‑1 (MQ=255)
                                                                                                                                            ccGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATta   <  1:2889325/148‑1 (MQ=255)
                                                                                                                                             cGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATtag  <  1:2795641/148‑1 (MQ=255)
                                                                                                                                             cGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATtag  >  2:2559919/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAG  >  CP009273/4176679‑4176967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCG  >  CP009273/4176674‑4176972
                                                                                                                                                     |                                                                                                                                                     
TGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTT                                                                                                                                                       >  SRR19973066.1591458/1‑150 (MQ=60)
    TGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTG                                                                                                                                                   >  SRR19973066.756911/1‑150 (MQ=60)
    TGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTG                                                                                                                                                   >  SRR19973066.756918/1‑150 (MQ=60)
    TGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTG                                                                                                                                                   >  SRR19973066.841230/1‑150 (MQ=60)
     GTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGT                                                                                                                                                  >  SRR19973066.1200427/1‑150 (MQ=60)
         ACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTA                                                                                                                                              <  SRR19973066.2910431/150‑1 (MQ=60)
           GTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAAC                                                                                                                                            >  SRR19973066.1888728/1‑150 (MQ=60)
           GTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAAC                                                                                                                                            <  SRR19973066.2445945/150‑1 (MQ=60)
           GTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAAC                                                                                                                                            <  SRR19973066.1477448/150‑1 (MQ=60)
            TACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACG                                                                                                                                           <  SRR19973066.2364617/150‑1 (MQ=60)
                 CTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAA                                                                                                                                      >  SRR19973066.2206719/1‑150 (MQ=60)
                 CTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAA                                                                                                                                      >  SRR19973066.1309154/1‑150 (MQ=60)
                 CTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAA                                                                                                                                      >  SRR19973066.399057/1‑150 (MQ=60)
                  TGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAG                                                                                                                                     <  SRR19973066.951640/150‑1 (MQ=60)
                  TGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAG                                                                                                                                     <  SRR19973066.2744689/150‑1 (MQ=60)
                  TGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTCTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAG                                                                                                                                     >  SRR19973066.1937039/1‑150 (MQ=60)
                       CTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAA                                                                                                                                >  SRR19973066.1990798/1‑150 (MQ=60)
                        TGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAG                                                                                                                               >  SRR19973066.2054331/1‑150 (MQ=60)
                        TGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAG                                                                                                                               >  SRR19973066.2054293/1‑150 (MQ=60)
                                CAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTG                                                                                                                       <  SRR19973066.3440600/150‑1 (MQ=60)
                                       AAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTG                                                                                                                >  SRR19973066.1662874/1‑150 (MQ=60)
                                                  CTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAA                                                                                                     >  SRR19973066.2628069/1‑150 (MQ=60)
                                                  CTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAA                                                                                                     >  SRR19973066.3287019/1‑150 (MQ=60)
                                                   TGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAG                                                                                                    <  SRR19973066.736641/150‑1 (MQ=60)
                                                    GATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGC                                                                                                   <  SRR19973066.2525552/150‑1 (MQ=60)
                                                     ATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCA                                                                                                  >  SRR19973066.2322230/1‑150 (MQ=60)
                                                     ATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCA                                                                                                  >  SRR19973066.491533/1‑150 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              <  SRR19973066.733398/150‑1 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              <  SRR19973066.496196/150‑1 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              <  SRR19973066.2084353/150‑1 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              >  SRR19973066.1940671/1‑150 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              >  SRR19973066.1374837/1‑150 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              <  SRR19973066.160635/150‑1 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              <  SRR19973066.160628/150‑1 (MQ=60)
                                                          GTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACG                                                                                             >  SRR19973066.652516/1‑150 (MQ=60)
                                                            CTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTC                                                                                           <  SRR19973066.2044968/150‑1 (MQ=60)
                                                            CTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTC                                                                                           >  SRR19973066.1338148/1‑150 (MQ=60)
                                                             TACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCT                                                                                          >  SRR19973066.1858263/1‑150 (MQ=60)
                                                              ACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTG                                                                                         <  SRR19973066.1954566/150‑1 (MQ=60)
                                                                   CAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCG                                                                                    <  SRR19973066.1403730/150‑1 (MQ=60)
                                                                     ACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCT                                                                                  <  SRR19973066.844587/150‑1 (MQ=60)
                                                                      CATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTC                                                                                 >  SRR19973066.934203/1‑150 (MQ=60)
                                                                             TCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTG                                                                          <  SRR19973066.2293548/150‑1 (MQ=60)
                                                                                 CGGCGAACGGCGAACCAATCTTCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTT                                                                      <  SRR19973066.125632/150‑1 (MQ=60)
                                                                                 CGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTT                                                                      >  SRR19973066.839811/1‑150 (MQ=60)
                                                                                  GGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTC                                                                     >  SRR19973066.2344509/1‑150 (MQ=60)
                                                                                     GAACGGCGACCCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCT                                                                  <  SRR19973066.2527914/150‑1 (MQ=60)
                                                                                     GAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCT                                                                  <  SRR19973066.878397/150‑1 (MQ=60)
                                                                                            GAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCG                                                           >  SRR19973066.2402618/1‑150 (MQ=60)
                                                                                              ACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCG                                                         >  SRR19973066.387050/1‑150 (MQ=60)
                                                                                                 AATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGT                                                      >  SRR19973066.505212/1‑150 (MQ=60)
                                                                                                 AATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGT                                                      >  SRR19973066.1847493/1‑150 (MQ=60)
                                                                                                  ATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTT                                                     <  SRR19973066.2907041/150‑1 (MQ=60)
                                                                                                   TCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTA                                                    <  SRR19973066.1415523/150‑1 (MQ=60)
                                                                                                   TCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTA                                                    <  SRR19973066.1778936/150‑1 (MQ=60)
                                                                                                      TCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTACCGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAG                                                 <  SRR19973066.2173029/150‑1 (MQ=60)
                                                                                                      TCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAG                                                 <  SRR19973066.364544/150‑1 (MQ=60)
                                                                                                      TCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAG                                                 <  SRR19973066.112279/150‑1 (MQ=60)
                                                                                                      TCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAG                                                 <  SRR19973066.1926836/150‑1 (MQ=60)
                                                                                                       CGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTTTATCGCTCTGGTCTGGCTTCTCTGCCTGCGCGCGTTAAAGT                                                >  SRR19973066.3221210/1‑150 (MQ=60)
                                                                                                        GTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTG                                               <  SRR19973066.2415393/150‑1 (MQ=60)
                                                                                                         TTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCGTGCGCGCGTTAAAGTGC                                              <  SRR19973066.3350024/150‑1 (MQ=60)
                                                                                                         TTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGC                                              <  SRR19973066.3350034/150‑1 (MQ=60)
                                                                                                         TTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGC                                              <  SRR19973066.301833/150‑1 (MQ=60)
                                                                                                           CCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGT                                            >  SRR19973066.2484713/1‑150 (MQ=60)
                                                                                                             GTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTAT                                          <  SRR19973066.3338097/150‑1 (MQ=60)
                                                                                                               CTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCA                                        >  SRR19973066.3279823/1‑150 (MQ=60)
                                                                                                                 CAGGACGTTGTACTGGGTATGTACTACATGACCCGTTACTGTGTTAACGCCATAGTCGTAGTCATGGTGCTGACTGGCCCGATTGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACC                                      <  SRR19973066.1715737/150‑1 (MQ=60)
                                                                                                                  AGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCG                                     <  SRR19973066.534573/150‑1 (MQ=60)
                                                                                                                  AGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCG                                     >  SRR19973066.423293/1‑150 (MQ=60)
                                                                                                                      CGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCTTATCACCGAGTA                                 >  SRR19973066.276461/1‑150 (MQ=60)
                                                                                                                      CGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTA                                 >  SRR19973066.1323291/1‑150 (MQ=60)
                                                                                                                      CGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTA                                 >  SRR19973066.276378/1‑150 (MQ=60)
                                                                                                                       GTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTAT                                <  SRR19973066.3169177/150‑1 (MQ=60)
                                                                                                                       GTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTAT                                <  SRR19973066.1300223/150‑1 (MQ=60)
                                                                                                                        TTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATG                               <  SRR19973066.2095076/150‑1 (MQ=60)
                                                                                                                         TGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGA                              <  SRR19973066.2301407/150‑1 (MQ=60)
                                                                                                                          GTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAA                             <  SRR19973066.2949848/150‑1 (MQ=60)
                                                                                                                           TACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAA                            <  SRR19973066.1663495/150‑1 (MQ=60)
                                                                                                                            ACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAA                           <  SRR19973066.759723/150‑1 (MQ=60)
                                                                                                                                GGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGAT                       <  SRR19973066.3053468/150‑1 (MQ=60)
                                                                                                                                   CTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCT                    <  SRR19973066.1773996/150‑1 (MQ=60)
                                                                                                                                    TGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTA                   <  SRR19973066.292809/150‑1 (MQ=60)
                                                                                                                                       ACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACG                <  SRR19973066.1547302/150‑1 (MQ=60)
                                                                                                                                         TACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGT              >  SRR19973066.1398570/1‑150 (MQ=60)
                                                                                                                                            ATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAA           <  SRR19973066.2387026/150‑1 (MQ=60)
                                                                                                                                             TGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTGAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAT          >  SRR19973066.1749173/1‑150 (MQ=60)
                                                                                                                                                CCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAG       <  SRR19973066.704889/150‑1 (MQ=60)
                                                                                                                                                 CCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGT      <  SRR19973066.2908647/150‑1 (MQ=60)
                                                                                                                                                  CGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTA     <  SRR19973066.2814456/150‑1 (MQ=60)
                                                                                                                                                   GTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAG    >  SRR19973066.2983042/1‑150 (MQ=60)
                                                                                                                                                   GTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAG    <  SRR19973066.2795306/150‑1 (MQ=60)
                                                                                                                                                   GTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAG    <  SRR19973066.1003312/150‑1 (MQ=60)
                                                                                                                                                    TTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGC   >  SRR19973066.2453276/1‑150 (MQ=60)
                                                                                                                                                    TTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGC   >  SRR19973066.2734220/1‑150 (MQ=60)
                                                                                                                                                    TTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGC   >  SRR19973066.2734223/1‑150 (MQ=60)
                                                                                                                                                     TACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCG  <  SRR19973066.2252329/150‑1 (MQ=60)
                                                                                                                                                     TACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCG  <  SRR19973066.790395/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCG  >  CP009273/4176674‑4176972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑