Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,176,823 G→T 100% D516Y (GAC→TAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,176,8230GT98.8% 1104.6 / 2.2 335D516Y (GAC→TAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (140/189);  total (143/192)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 335 total aligned reads displayed.

GCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTA  >  CP009273/4176676‑4176969
                                                                                                                                                   |                                                                                                                                                  
 cTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACt                                                                                                                                                 >  2:742369/1‑150 (MQ=255)
 cTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACt                                                                                                                                                 <  1:2087183/150‑1 (MQ=255)
  tGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtg                                                                                                                                                >  2:4857160/1‑150 (MQ=255)
  tGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtg                                                                                                                                                <  1:90622/150‑1 (MQ=255)
   gTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtgt                                                                                                                                               <  2:1441882/150‑1 (MQ=255)
       aCGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTa                                                                                                                                           <  1:1188967/150‑1 (MQ=255)
       aCGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTa                                                                                                                                           <  2:1024568/150‑1 (MQ=255)
           aCCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGc                                                                                                                                       <  1:2503728/150‑1 (MQ=255)
                tGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAg                                                                                                                                  <  1:4682167/150‑1 (MQ=255)
                     cTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGaa                                                                                                                             <  2:867825/150‑1 (MQ=255)
                      tGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAg                                                                                                                            >  1:1452829/1‑150 (MQ=255)
                       ggAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAgg                                                                                                                           <  2:2857007/150‑1 (MQ=255)
                         aaGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCa                                                                                                                         >  1:4096414/1‑150 (MQ=255)
                              cAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTg                                                                                                                    <  1:4408003/150‑1 (MQ=255)
                               aGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGc                                                                                                                   <  1:719865/150‑1 (MQ=255)
                               aGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGc                                                                                                                   <  1:2180176/150‑1 (MQ=255)
                                 cTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTg                                                                                                                 >  1:3856089/1‑150 (MQ=255)
                                  tGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGa                                                                                                                >  2:2293980/1‑150 (MQ=255)
                                  tGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGa                                                                                                                >  2:617835/1‑150 (MQ=255)
                                   ggAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                                                                                                               >  2:2981787/1‑150 (MQ=255)
                                    gAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGGTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACt                                                                                                              <  2:52379/150‑1 (MQ=255)
                                     aaGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTg                                                                                                             <  1:3529720/150‑1 (MQ=255)
                                      aGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTgg                                                                                                            >  1:2648130/1‑150 (MQ=255)
                                       gcgcGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGc                                                                                                           <  1:3373644/150‑1 (MQ=255)
                                        cgcgTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGcc                                                                                                          >  1:4920482/1‑150 (MQ=255)
                                         gcgTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGccc                                                                                                         <  2:325654/150‑1 (MQ=255)
                                            tGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGaa                                                                                                      >  1:2207748/1‑150 (MQ=255)
                                            tGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGaa                                                                                                      <  1:1428165/150‑1 (MQ=255)
                                                    tgatgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAg                                                                                              >  2:1742817/1‑150 (MQ=255)
                                                      atgTCTACCAACAACATCCTGTCCCCGGTGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGaa                                                                                            >  2:1320722/1‑150 (MQ=255)
                                                      atgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGaa                                                                                            <  2:218602/150‑1 (MQ=255)
                                                      atgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGaa                                                                                            <  2:148965/150‑1 (MQ=255)
                                                       tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAc                                                                                           <  2:2346387/150‑1 (MQ=255)
                                                       tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAc                                                                                           <  1:2621739/150‑1 (MQ=255)
                                                       tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAc                                                                                           >  2:578306/1‑150 (MQ=255)
                                                       agTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCACAGGACGTGGTACTGGGTCTGTACAACATTGCCCGTAACTGTGTTAACGCCCAAGGCGACGGCAATGTGATCTCTGTCCCGAAAGACGCAGaaa                                                                                           >  2:2677758/2‑149 (MQ=255)
                                                           tACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCt                                                                                       <  2:4434685/150‑1 (MQ=255)
                                                                 caacaTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCg                                                                                 <  2:1993236/150‑1 (MQ=255)
                                                                 caacaTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCg                                                                                 >  2:2334448/1‑150 (MQ=255)
                                                                       ccTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctgg                                                                           >  2:3955965/1‑150 (MQ=255)
                                                                         tGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtc                                                                         >  2:115212/1‑150 (MQ=255)
                                                                           tCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtctg                                                                       <  1:270987/150‑1 (MQ=255)
                                                                            ccccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtctgg                                                                      >  1:406056/1‑150 (MQ=255)
                                                                            ccccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtctgg                                                                      <  1:3304237/150‑1 (MQ=255)
                                                                            ccccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtctgg                                                                      >  1:54737/1‑150 (MQ=255)
                                                                              ccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCt                                                                    <  1:1045355/150‑1 (MQ=255)
                                                                              ccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCt                                                                    <  1:51492/150‑1 (MQ=255)
                                                                              ccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCt                                                                    >  1:4238153/1‑150 (MQ=255)
                                                                               cggcgaacggcgaaCCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCtt                                                                   <  2:3451026/150‑1 (MQ=255)
                                                                               cggcgaacggcgaaCCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCtt                                                                   <  1:5010685/150‑1 (MQ=255)
                                                                                  cgaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTtctc                                                                >  2:3205801/1‑150 (MQ=255)
                                                                                   gaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTctct                                                               <  1:4830502/150‑1 (MQ=255)
                                                                                   gaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTctct                                                               <  1:1488739/150‑1 (MQ=255)
                                                                                   gaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTctct                                                               >  2:5053899/1‑150 (MQ=255)
                                                                                      cggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCa                                                            >  1:2289573/1‑150 (MQ=255)
                                                                                       ggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCAt                                                           <  2:1109262/150‑1 (MQ=255)
                                                                                        gcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATg                                                          <  1:2373010/150‑1 (MQ=255)
                                                                                        gcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATg                                                          <  1:4225807/150‑1 (MQ=255)
                                                                                         cgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgc                                                         >  2:2089582/1‑150 (MQ=255)
                                                                                         cgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgc                                                         <  1:3658056/150‑1 (MQ=255)
                                                                                          gaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcg                                                        >  1:4285269/1‑150 (MQ=255)
                                                                                          gaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcg                                                        >  1:3422443/1‑150 (MQ=255)
                                                                                            acCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcgcg                                                      <  1:1654220/150‑1 (MQ=255)
                                                                                              cAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGcgcgcg                                                    <  1:1280088/150‑1 (MQ=255)
                                                                                                atcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGtt                                                  <  2:4583264/150‑1 (MQ=255)
                                                                                                atcatcGGTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGtt                                                  <  1:1016031/150‑1 (MQ=255)
                                                                                                 tcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTa                                                 <  1:3712190/150‑1 (MQ=255)
                                                                                                 tcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTa                                                 >  1:5047936/1‑150 (MQ=255)
                                                                                                      gTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                            >  1:1677725/1‑150 (MQ=255)
                                                                                                      gTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                            >  1:2962189/1‑150 (MQ=255)
                                                                                                       ttCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGc                                           >  2:3569176/1‑150 (MQ=255)
                                                                                                        tCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCg                                          <  2:4622215/150‑1 (MQ=255)
                                                                                                        tCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCg                                          >  1:2069911/1‑150 (MQ=255)
                                                                                                          cGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTa                                        <  2:3817080/150‑1 (MQ=255)
                                                                                                          cGTCTCAGGAAGTTGTACTGGGTCTGTACTACATGACCCGGTACTGGGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTa                                        >  2:4356990/1‑150 (MQ=255)
                                                                                                             ctcAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCa                                     <  2:1740783/150‑1 (MQ=255)
                                                                                                             ctcAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCa                                     >  2:4727280/1‑150 (MQ=255)
                                                                                                                 ggACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGa                                 <  2:3388053/150‑1 (MQ=255)
                                                                                                                   aCGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGt                               <  2:2547609/150‑1 (MQ=255)
                                                                                                                     gTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTAt                             <  2:2523768/150‑1 (MQ=255)
                                                                                                                        gTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaa                          >  1:4441029/1‑150 (MQ=255)
                                                                                                                        gTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaa                          >  1:3149970/1‑150 (MQ=255)
                                                                                                                        gTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaa                          >  1:562684/1‑150 (MQ=255)
                                                                                                                          aCTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGaaaa                        >  2:2844530/1‑150 (MQ=255)
                                                                                                                            tGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAg                      >  1:1338441/1‑150 (MQ=255)
                                                                                                                             gggTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGa                     >  2:4076973/1‑150 (MQ=255)
                                                                                                                              ggTCAGTACTACATGACCCGTTACGGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGAt                    >  1:1841235/1‑150 (MQ=255)
                                                                                                                               gTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATg                   <  1:5081425/150‑1 (MQ=255)
                                                                                                                                tCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGc                  <  1:79005/150‑1 (MQ=255)
                                                                                                                                 cTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCt                 <  2:2771481/150‑1 (MQ=255)
                                                                                                                                 cTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCt                 <  1:4171248/150‑1 (MQ=255)
                                                                                                                                 cTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCt                 <  2:1628165/150‑1 (MQ=255)
                                                                                                                                      ctacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACgg            <  2:1888209/150‑1 (MQ=255)
                                                                                                                                        acATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTg          <  1:3428352/150‑1 (MQ=255)
                                                                                                                                           tGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAt       <  2:4163065/150‑1 (MQ=255)
                                                                                                                                             aCCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATta     <  2:4238664/150‑1 (MQ=255)
                                                                                                                                             aCCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATta     <  1:3192594/150‑1 (MQ=255)
                                                                                                                                             aCCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATta     <  2:1541715/150‑1 (MQ=255)
                                                                                                                                               ccGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATtagt   >  2:3147043/1‑150 (MQ=255)
                                                                                                                                                cGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATtagta  <  2:36697/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTA  >  CP009273/4176676‑4176969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCG  >  CP009273/4176674‑4176972
                                                                                                                                                     |                                                                                                                                                     
TGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTT                                                                                                                                                       <  SRR19973063.2048158/150‑1 (MQ=60)
TGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTT                                                                                                                                                       >  SRR19973063.3058479/1‑150 (MQ=60)
TGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTT                                                                                                                                                       >  SRR19973063.520357/1‑150 (MQ=60)
 GGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTA                                                                                                                                                      <  SRR19973063.1713358/150‑1 (MQ=60)
   CTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACT                                                                                                                                                    <  SRR19973063.2093704/150‑1 (MQ=60)
   CTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACT                                                                                                                                                    <  SRR19973063.914182/150‑1 (MQ=60)
    TGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTG                                                                                                                                                   <  SRR19973063.90928/150‑1 (MQ=60)
       TCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGT                                                                                                                                                <  SRR19973063.4622241/150‑1 (MQ=60)
         ACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTA                                                                                                                                              <  SRR19973063.1192756/150‑1 (MQ=60)
         ACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTA                                                                                                                                              <  SRR19973063.1192541/150‑1 (MQ=60)
            TACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACG                                                                                                                                           >  SRR19973063.1578531/1‑150 (MQ=60)
             ACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGC                                                                                                                                          <  SRR19973063.2511714/150‑1 (MQ=60)
                  TGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAG                                                                                                                                     <  SRR19973063.1733141/150‑1 (MQ=60)
                  TGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAG                                                                                                                                     <  SRR19973063.923205/150‑1 (MQ=60)
                  TGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAG                                                                                                                                     <  SRR19973063.1733171/150‑1 (MQ=60)
                  TGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAG                                                                                                                                     <  SRR19973063.4697095/150‑1 (MQ=60)
                     CGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCG                                                                                                                                  >  SRR19973063.4252176/1‑150 (MQ=60)
                        TGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAG                                                                                                                               >  SRR19973063.1457258/1‑150 (MQ=60)
                        TGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAG                                                                                                                               >  SRR19973063.1457261/1‑150 (MQ=60)
                        TGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAG                                                                                                                               <  SRR19973063.2766468/150‑1 (MQ=60)
                         GGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGG                                                                                                                              <  SRR19973063.2217650/150‑1 (MQ=60)
                         GGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGG                                                                                                                              <  SRR19973063.4978781/150‑1 (MQ=60)
                          GAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGC                                                                                                                             <  SRR19973063.2619213/150‑1 (MQ=60)
                           AAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCA                                                                                                                            <  SRR19973063.3226278/150‑1 (MQ=60)
                           AAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCA                                                                                                                            >  SRR19973063.1173543/1‑150 (MQ=60)
                           AAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCA                                                                                                                            >  SRR19973063.4109540/1‑150 (MQ=60)
                            AGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCAT                                                                                                                           >  SRR19973063.1666584/1‑150 (MQ=60)
                            AGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCAT                                                                                                                           >  SRR19973063.1666618/1‑150 (MQ=60)
                            AGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCAT                                                                                                                           <  SRR19973063.5052722/150‑1 (MQ=60)
                               CTAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGT                                                                                                                        >  SRR19973063.3053691/1‑150 (MQ=60)
                               CCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGT                                                                                                                        >  SRR19973063.5066485/1‑150 (MQ=60)
                               CCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGT                                                                                                                        >  SRR19973063.4330753/1‑150 (MQ=60)
                                CAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTG                                                                                                                       <  SRR19973063.4422140/150‑1 (MQ=60)
                                CAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTG                                                                                                                       >  SRR19973063.5148760/1‑150 (MQ=60)
                                 AGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACCTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCTTGGTGC                                                                                                                      <  SRR19973063.2186996/150‑1 (MQ=60)
                                 AGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGC                                                                                                                      <  SRR19973063.3168277/150‑1 (MQ=60)
                                 AGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGC                                                                                                                      <  SRR19973063.2186997/150‑1 (MQ=60)
                                 AGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGC                                                                                                                      <  SRR19973063.721983/150‑1 (MQ=60)
                                  GCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCT                                                                                                                     <  SRR19973063.3900502/150‑1 (MQ=60)
                                  GCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCT                                                                                                                     <  SRR19973063.647252/150‑1 (MQ=60)
                                   CTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTG                                                                                                                    >  SRR19973063.4067892/1‑150 (MQ=60)
                                   CTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTG                                                                                                                    >  SRR19973063.3868350/1‑150 (MQ=60)
                                    TGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGA                                                                                                                   >  SRR19973063.3548219/1‑150 (MQ=60)
                                      GAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACT                                                                                                                 >  SRR19973063.2078366/1‑150 (MQ=60)
                                       AAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTG                                                                                                                >  SRR19973063.4249619/1‑150 (MQ=60)
                                       AAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTG                                                                                                                <  SRR19973063.3541022/150‑1 (MQ=60)
                                        ATCGCGTGCGCAGATGATGTCTACAAACAAAATCATGTCCACGGCTAACGCAGAACCAAACATCGTTCCGTCTCAGTACTATGTAATGGGACTGTACTACATGACACGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGT                                                                                                               >  SRR19973063.2656592/1‑150 (MQ=60)
                                        AGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGG                                                                                                               >  SRR19973063.2656598/1‑150 (MQ=60)
                                        AGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGG                                                                                                               >  SRR19973063.2656600/1‑150 (MQ=60)
                                         GCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGC                                                                                                              <  SRR19973063.3384389/150‑1 (MQ=60)
                                         GCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGC                                                                                                              <  SRR19973063.2254963/150‑1 (MQ=60)
                                          CGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCC                                                                                                             >  SRR19973063.4936374/1‑150 (MQ=60)
                                           TCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCC                                                                                                            <  SRR19973063.1449778/150‑1 (MQ=60)
                                           GCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCC                                                                                                            <  SRR19973063.1449608/150‑1 (MQ=60)
                                             GTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGA                                                                                                          >  SRR19973063.3435911/1‑150 (MQ=60)
                                             GTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGA                                                                                                          <  SRR19973063.4515195/150‑1 (MQ=60)
                                              TGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCACTCTTCGGTCCGTCTCAGGACGTTGTTCTGGGTCTGTACTACTTGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAA                                                                                                         <  SRR19973063.1432526/150‑1 (MQ=60)
                                              TGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAA                                                                                                         <  SRR19973063.1432518/150‑1 (MQ=60)
                                              TGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAA                                                                                                         >  SRR19973063.2214703/1‑150 (MQ=60)
                                                    GATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGC                                                                                                   <  SRR19973063.3574125/150‑1 (MQ=60)
                                                     ATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCA                                                                                                  <  SRR19973063.2712151/150‑1 (MQ=60)
                                                     ATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCA                                                                                                  <  SRR19973063.5176021/150‑1 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              <  SRR19973063.2630088/150‑1 (MQ=60)
                                                         TGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAC                                                                                              <  SRR19973063.2630091/150‑1 (MQ=60)
                                                          GTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACG                                                                                             >  SRR19973063.1977740/1‑150 (MQ=60)
                                                               CCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGT                                                                                        >  SRR19973063.3207822/1‑150 (MQ=60)
                                                                 AACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTAT                                                                                      >  SRR19973063.52567/1‑150 (MQ=60)
                                                                  ACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATC                                                                                     <  SRR19973063.3328098/150‑1 (MQ=60)
                                                                   CAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCG                                                                                    >  SRR19973063.1483417/1‑150 (MQ=60)
                                                                   CAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCG                                                                                    >  SRR19973063.3524884/1‑150 (MQ=60)
                                                                        TCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTG                                                                               <  SRR19973063.4241732/150‑1 (MQ=60)
                                                                          CTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGATGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGT                                                                             <  SRR19973063.2469661/150‑1 (MQ=60)
                                                                            GTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCT                                                                           >  SRR19973063.3398846/1‑150 (MQ=60)
                                                                             TCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTG                                                                          <  SRR19973063.1248867/150‑1 (MQ=60)
                                                                             TCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTG                                                                          <  SRR19973063.271771/150‑1 (MQ=60)
                                                                             TCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTG                                                                          <  SRR19973063.271790/150‑1 (MQ=60)
                                                                              CCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGGACGTCTGTATCGCTCTGGTCTGG                                                                         <  SRR19973063.3314850/150‑1 (MQ=60)
                                                                              CCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGG                                                                         <  SRR19973063.3314809/150‑1 (MQ=60)
                                                                              CCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGG                                                                         >  SRR19973063.54928/1‑150 (MQ=60)
                                                                              CCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGG                                                                         >  SRR19973063.407201/1‑150 (MQ=60)
                                                                              CCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGG                                                                         >  SRR19973063.54950/1‑150 (MQ=60)
                                                                                CCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCT                                                                       <  SRR19973063.1328664/150‑1 (MQ=60)
                                                                                CCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCT                                                                       <  SRR19973063.5126682/150‑1 (MQ=60)
                                                                                CCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCT                                                                       <  SRR19973063.1328682/150‑1 (MQ=60)
                                                                                CCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCT                                                                       <  SRR19973063.51676/150‑1 (MQ=60)
                                                                                CCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCT                                                                       <  SRR19973063.1048520/150‑1 (MQ=60)
                                                                                CCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCT                                                                       >  SRR19973063.4251664/1‑150 (MQ=60)
                                                                                 CGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTT                                                                      <  SRR19973063.5026762/150‑1 (MQ=60)
                                                                                 CGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTT                                                                      <  SRR19973063.5026857/150‑1 (MQ=60)
                                                                                 CGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTT                                                                      <  SRR19973063.2200292/150‑1 (MQ=60)
                                                                                 CGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTT                                                                      <  SRR19973063.5026871/150‑1 (MQ=60)
                                                                                   GCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCT                                                                    <  SRR19973063.1024384/150‑1 (MQ=60)
                                                                                   GCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCT                                                                    <  SRR19973063.1024395/150‑1 (MQ=60)
                                                                                    CGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTC                                                                   >  SRR19973063.1397346/1‑150 (MQ=60)
                                                                                     GAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCT                                                                  <  SRR19973063.1493298/150‑1 (MQ=60)
                                                                                     GAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCT                                                                  >  SRR19973063.4230326/1‑150 (MQ=60)
                                                                                     GAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCT                                                                  <  SRR19973063.4845993/150‑1 (MQ=60)
                                                                                     GAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCT                                                                  >  SRR19973063.708263/1‑150 (MQ=60)
                                                                                        CGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCA                                                               >  SRR19973063.2296883/1‑150 (MQ=60)
                                                                                          GCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATG                                                             <  SRR19973063.4303749/150‑1 (MQ=60)
                                                                                          GCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATG                                                             <  SRR19973063.2380689/150‑1 (MQ=60)
                                                                                          GCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATG                                                             <  SRR19973063.2380613/150‑1 (MQ=60)
                                                                                          GCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATG                                                             <  SRR19973063.4239276/150‑1 (MQ=60)
                                                                                           CGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGC                                                            <  SRR19973063.3669784/150‑1 (MQ=60)
                                                                                            GAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCG                                                           >  SRR19973063.3433330/1‑150 (MQ=60)
                                                                                            GAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCG                                                           >  SRR19973063.4709096/1‑150 (MQ=60)
                                                                                            GAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCG                                                           >  SRR19973063.763142/1‑150 (MQ=60)
                                                                                            GAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCG                                                           >  SRR19973063.4953717/1‑150 (MQ=60)
                                                                                            GAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCG                                                           >  SRR19973063.4298882/1‑150 (MQ=60)
                                                                                              ACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCG                                                         <  SRR19973063.2241423/150‑1 (MQ=60)
                                                                                              ACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCG                                                         <  SRR19973063.3673634/150‑1 (MQ=60)
                                                                                              ACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCG                                                         <  SRR19973063.1659268/150‑1 (MQ=60)
                                                                                              ACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCG                                                         <  SRR19973063.838276/150‑1 (MQ=60)
                                                                                                CAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCG                                                       >  SRR19973063.3822516/1‑150 (MQ=60)
                                                                                                CAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCG                                                       <  SRR19973063.1283905/150‑1 (MQ=60)
                                                                                                  ATCATCGGTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTT                                                     <  SRR19973063.1019095/150‑1 (MQ=60)
                                                                                                   TCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTA                                                    <  SRR19973063.3724058/150‑1 (MQ=60)
                                                                                                   TCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTA                                                    >  SRR19973063.5064196/1‑150 (MQ=60)
                                                                                                   TCATCGGTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTA                                                    <  SRR19973063.3411197/150‑1 (MQ=60)
                                                                                                     ATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAA                                                  <  SRR19973063.3165798/150‑1 (MQ=60)
                                                                                                     ATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAA                                                  >  SRR19973063.4306204/1‑150 (MQ=60)
                                                                                                        GTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTG                                               >  SRR19973063.1682838/1‑150 (MQ=60)
                                                                                                        GTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTG                                               >  SRR19973063.2971660/1‑150 (MQ=60)
                                                                                                         TTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGC                                              >  SRR19973063.1673677/1‑150 (MQ=60)
                                                                                                         TTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGC                                              >  SRR19973063.4258679/1‑150 (MQ=60)
                                                                                                          TCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCG                                             >  SRR19973063.2076356/1‑150 (MQ=60)
                                                                                                          TCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCG                                             <  SRR19973063.3901745/150‑1 (MQ=60)
                                                                                                          TCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCG                                             >  SRR19973063.2076381/1‑150 (MQ=60)
                                                                                                           CCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGT                                            <  SRR19973063.2317358/150‑1 (MQ=60)
                                                                                                            CGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTA                                           <  SRR19973063.998996/150‑1 (MQ=60)
                                                                                                              TCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATC                                         >  SRR19973063.4142822/1‑150 (MQ=60)
                                                                                                               CTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCA                                        <  SRR19973063.2622820/150‑1 (MQ=60)
                                                                                                               CTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCA                                        <  SRR19973063.2622664/150‑1 (MQ=60)
                                                                                                                TCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCAC                                       >  SRR19973063.1418650/1‑150 (MQ=60)
                                                                                                                TCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCAC                                       >  SRR19973063.2118244/1‑150 (MQ=60)
                                                                                                                 CAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACC                                      <  SRR19973063.4139683/150‑1 (MQ=60)
                                                                                                                 CAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACC                                      <  SRR19973063.58214/150‑1 (MQ=60)
                                                                                                                  AGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCG                                     <  SRR19973063.3729070/150‑1 (MQ=60)
                                                                                                                     ACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGT                                  <  SRR19973063.2193425/150‑1 (MQ=60)
                                                                                                                       GTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTAT                                >  SRR19973063.1130180/1‑150 (MQ=60)
                                                                                                                       GTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTAT                                <  SRR19973063.3684446/150‑1 (MQ=60)
                                                                                                                       GTTGTACTGGGTATGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCTGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTAT                                <  SRR19973063.3419069/150‑1 (MQ=60)
                                                                                                                        TTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATG                               >  SRR19973063.560231/1‑150 (MQ=60)
                                                                                                                          GTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAA                             >  SRR19973063.3160095/1‑150 (MQ=60)
                                                                                                                          GTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAA                             >  SRR19973063.564346/1‑150 (MQ=60)
                                                                                                                          GTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAA                             >  SRR19973063.4455280/1‑150 (MQ=60)
                                                                                                                          GTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAA                             >  SRR19973063.4455270/1‑150 (MQ=60)
                                                                                                                           TACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAA                            <  SRR19973063.161955/150‑1 (MQ=60)
                                                                                                                            ACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAA                           >  SRR19973063.2089653/1‑150 (MQ=60)
                                                                                                                            ACTGGGTCTGTACTACATGACCCGTAACTGTCTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCATAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGACAA                           >  SRR19973063.2089272/1‑150 (MQ=60)
                                                                                                                             CTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAA                          <  SRR19973063.1282672/150‑1 (MQ=60)
                                                                                                                              TGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAG                         >  SRR19973063.1342455/1‑150 (MQ=60)
                                                                                                                                GGTCAGTACTACATGACCCGTTACGGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGAT                       >  SRR19973063.1846979/1‑150 (MQ=60)
                                                                                                                                 GTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATG                      <  SRR19973063.5097770/150‑1 (MQ=60)
                                                                                                                                 GTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATG                      <  SRR19973063.5097743/150‑1 (MQ=60)
                                                                                                                                 GTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATG                      <  SRR19973063.4907830/150‑1 (MQ=60)
                                                                                                                                  TCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGC                     <  SRR19973063.79276/150‑1 (MQ=60)
                                                                                                                                   CTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCT                    <  SRR19973063.4184579/150‑1 (MQ=60)
                                                                                                                                   CTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCT                    >  SRR19973063.2991897/1‑150 (MQ=60)
                                                                                                                                   CTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCT                    >  SRR19973063.3561520/1‑150 (MQ=60)
                                                                                                                                   CTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCT                    >  SRR19973063.3435939/1‑150 (MQ=60)
                                                                                                                                     GTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAA                  <  SRR19973063.744545/150‑1 (MQ=60)
                                                                                                                                      TACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAAC                 <  SRR19973063.4090034/150‑1 (MQ=60)
                                                                                                                                        CTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGG               <  SRR19973063.4208512/150‑1 (MQ=60)
                                                                                                                                         TACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGT              <  SRR19973063.422336/150‑1 (MQ=60)
                                                                                                                                         TACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGT              <  SRR19973063.5100715/150‑1 (MQ=60)
                                                                                                                                         TACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGT              <  SRR19973063.4587376/150‑1 (MQ=60)
                                                                                                                                          ACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTG             <  SRR19973063.3439256/150‑1 (MQ=60)
                                                                                                                                          ACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTG             <  SRR19973063.3439265/150‑1 (MQ=60)
                                                                                                                                           CATTACCCGTTTCTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTTGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGA            <  SRR19973063.4448766/150‑1 (MQ=60)
                                                                                                                                             TGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAT          >  SRR19973063.2823904/1‑150 (MQ=60)
                                                                                                                                             TGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAT          >  SRR19973063.4093373/1‑150 (MQ=60)
                                                                                                                                               ACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTA        <  SRR19973063.3202859/150‑1 (MQ=60)
                                                                                                                                               ACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTA        <  SRR19973063.3202797/150‑1 (MQ=60)
                                                                                                                                               ACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTA        <  SRR19973063.2360456/150‑1 (MQ=60)
                                                                                                                                                CCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAG       <  SRR19973063.4872807/150‑1 (MQ=60)
                                                                                                                                                CCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAG       >  SRR19973063.4745896/1‑150 (MQ=60)
                                                                                                                                                 CCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGT      >  SRR19973063.816208/1‑150 (MQ=60)
                                                                                                                                                    TTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGC   >  SRR19973063.629832/1‑150 (MQ=60)
                                                                                                                                                    TTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGC   <  SRR19973063.5067440/150‑1 (MQ=60)
                                                                                                                                                     TACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCG  <  SRR19973063.2341913/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTAGCG  >  CP009273/4176674‑4176972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑