Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F142 I0 R1	859	274.6	8723680	96.4%	8409627	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,313,924	C→A	21.1%	R135S (CGC→AGC)	atoS →	sensory histidine kinase in two‑component regulatory system with AtoC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,313,924	0	C	A	21.1%	637.3 / 123.9	238	R135S (CGC→AGC)	atoS	sensory histidine kinase in two‑component regulatory system with AtoC
Reads supporting (aligned to +/- strand): ref base C (73/114); new base A (24/27); total (97/141)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

CCCCCATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTCGCCGCCAG  >  CP009273/2313778‑2314073

cccccATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGCTCACCCTGGTcgcg                                                                                                                                                    >  1:4206542/1‑150 (MQ=255)

  cccATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGTGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGaa                                                                                                                                                  <  1:1125646/150‑1 (MQ=255)

   ccATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAg                                                                                                                                                 <  1:3319415/150‑1 (MQ=255)

     aTTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTg                                                                                                                                               <  1:3868694/150‑1 (MQ=255)

       tACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGAt                                                                                                                                             <  1:987259/150‑1 (MQ=255)

       tACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGAt                                                                                                                                             <  1:3038805/150‑1 (MQ=255)

        aCCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATg                                                                                                                                            >  1:2658031/1‑150 (MQ=255)

          cGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCg                                                                                                                                          >  2:1772715/1‑150 (MQ=255)

           gAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGt                                                                                                                                         <  1:660437/150‑1 (MQ=255)

           gAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGt                                                                                                                                         >  1:4081818/1‑150 (MQ=255)

           gAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGt                                                                                                                                         <  1:3561558/150‑1 (MQ=255)

            aaaaTATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTa                                                                                                                                        <  2:274052/150‑1 (MQ=255)

            aaaaTATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTa                                                                                                                                        >  1:876607/1‑150 (MQ=255)

             aaaTATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTAc                                                                                                                                       <  1:558287/150‑1 (MQ=255)

                 atCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAAt                                                                                                                                   <  1:1854276/150‑1 (MQ=255)

                   aactcacGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTAACATTGCCGCAGATCACCCTGGTCGCGACGTGATGCGGACCAATAc                                                                                                                                 >  2:3815148/2‑150 (MQ=255)

                         cGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCttt                                                                                                                           <  1:3618249/150‑1 (MQ=255)

                          gCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTa                                                                                                                          >  1:2000891/1‑150 (MQ=255)

                           ccgtCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAg                                                                                                                         <  2:1974706/147‑1 (MQ=255)

                           ccTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTGTAg                                                                                                                         >  2:1340071/1‑150 (MQ=255)

                             ttCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGtt                                                                                                                       >  1:1547376/1‑150 (MQ=255)

                               cccTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTa                                                                                                                     >  1:1968024/1‑150 (MQ=255)

                                  tGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTc                                                                                                                  >  1:3943016/1‑150 (MQ=255)

                                  tGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTc                                                                                                                  >  1:3234289/1‑150 (MQ=255)

                                    gCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcag                                                                                                                >  2:3767762/1‑150 (MQ=255)

                                     cATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcagg                                                                                                               <  1:2524743/150‑1 (MQ=255)

                                       tCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggca                                                                                                             <  2:2930651/150‑1 (MQ=255)

                                       tCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggca                                                                                                             <  1:2325312/150‑1 (MQ=255)

                                        cGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcag                                                                                                            <  2:1185691/150‑1 (MQ=255)

                                          gTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcaggc                                                                                                          <  1:592987/150‑1 (MQ=255)

                                           tGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcaggca                                                                                                         >  1:813959/1‑150 (MQ=255)

                                           tGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCGTTAGTTTATTcaggcaggca                                                                                                         >  1:3326276/1‑150 (MQ=255)

                                            gCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcaggcag                                                                                                        >  1:2713243/1‑150 (MQ=255)

                                             cTGGTTATTACAACAACAGGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcaggcagg                                                                                                       <  2:2423970/150‑1 (MQ=255)

                                             cTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcaggcagg                                                                                                       >  1:1822206/1‑150 (MQ=255)

                                              tGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGt                                                                                                      >  1:3196828/1‑150 (MQ=255)

                                                gTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCATTAGTATATTAAGGCAGGCAGGTgc                                                                                                    >  1:3316418/1‑150 (MQ=255)

                                                     taCAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCgcggc                                                                                               >  2:2767715/1‑150 (MQ=255)

                                                      acaacaAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCgcggcg                                                                                              <  2:1822673/150‑1 (MQ=255)

                                                       caacaaAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGa                                                                                             <  2:1269995/150‑1 (MQ=255)

                                                        aacaaAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGat                                                                                            >  1:533377/1‑150 (MQ=255)

                                                         acaaAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGata                                                                                           >  2:3152881/1‑150 (MQ=255)

                                                         acaaAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGata                                                                                           <  1:3952547/150‑1 (MQ=255)

                                                             aaTGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATAtttt                                                                                       <  1:1519625/150‑1 (MQ=255)

                                                              aTGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTg                                                                                      <  1:318318/150‑1 (MQ=255)

                                                                   ggATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTc                                                                                 <  2:2802510/150‑1 (MQ=255)

                                                                              tCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCa                                                                      <  1:1835721/150‑1 (MQ=255)

                                                                                 ccTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTg                                                                   <  2:4139247/150‑1 (MQ=255)

                                                                                  cTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGa                                                                  >  1:1063125/1‑150 (MQ=255)

                                                                                   tACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAg                                                                 <  1:3495083/150‑1 (MQ=255)

                                                                                     cgcgCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCg                                                               <  1:1694904/150‑1 (MQ=255)

                                                                                      gcgcCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGt                                                              <  1:4145167/150‑1 (MQ=255)

                                                                                      gcgcCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGt                                                              >  2:554554/1‑150 (MQ=255)

                                                                                        gcCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTaa                                                            <  1:269226/150‑1 (MQ=255)

                                                                                         ccTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAAt                                                           >  2:1179577/1‑150 (MQ=255)

                                                                                             cAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTg                                                       <  1:2869019/150‑1 (MQ=255)

                                                                                              cgcgcTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGa                                                      >  1:2979465/2‑150 (MQ=255)

                                                                                              aGCGCTATATCAGAATAATGTCGGCGTTACCCTTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAAGGCTCCCCATTGAGCGTAATGGTGa                                                      <  2:666460/150‑1 (MQ=255)

                                                                                              aGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGa                                                      <  1:2912862/150‑1 (MQ=255)

                                                                                               gcgcTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGaa                                                     <  2:615407/150‑1 (MQ=255)

                                                                                                cgcTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGaaa                                                    <  2:2128573/150‑1 (MQ=255)

                                                                                                 gcTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAAt                                                   <  2:1999839/150‑1 (MQ=255)

                                                                                                  cTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATc                                                  <  1:2705539/150‑1 (MQ=255)

                                                                                                   tataTCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATcc                                                 >  2:1519506/1‑150 (MQ=255)

                                                                                                     tatCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTc                                               >  2:3471085/1‑150 (MQ=255)

                                                                                                      atCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCg                                              <  2:2600043/150‑1 (MQ=255)

                                                                                                       tCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCgg                                             >  2:2700282/1‑150 (MQ=255)

                                                                                                         aGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCt                                           >  1:2304477/1‑150 (MQ=255)

                                                                                                         aGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCt                                           >  1:4339847/1‑150 (MQ=255)

                                                                                                          gAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCta                                          <  2:394137/150‑1 (MQ=255)

                                                                                                          gAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCta                                          <  2:394222/150‑1 (MQ=255)

                                                                                                           aataatGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCtat                                         <  1:3781235/150‑1 (MQ=255)

                                                                                                             taatGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTatat                                       <  2:172913/150‑1 (MQ=255)

                                                                                                             taatGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTatat                                       <  1:1464526/150‑1 (MQ=255)

                                                                                                               atGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCt                                     <  2:2459739/150‑1 (MQ=255)

                                                                                                               atGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCt                                     >  2:1049688/1‑150 (MQ=255)

                                                                                                               atGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCt                                     <  2:285403/150‑1 (MQ=255)

                                                                                                                tGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTg                                    >  1:3528664/1‑150 (MQ=255)

                                                                                                                      cGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCaa                              <  1:136638/150‑1 (MQ=255)

                                                                                                                        ttACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATg                            >  1:1761958/1‑150 (MQ=255)

                                                                                                                         tACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGa                           >  1:4039760/1‑150 (MQ=255)

                                                                                                                          aCCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGaa                          >  2:2148391/1‑150 (MQ=255)

                                                                                                                            cATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAAtt                        <  1:3969243/150‑1 (MQ=255)

                                                                                                                                gCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAAcc                    <  2:2323783/150‑1 (MQ=255)

                                                                                                                                gCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAAcc                    <  2:3385340/150‑1 (MQ=255)

                                                                                                                                 ccGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCg                   <  1:2107550/150‑1 (MQ=255)

                                                                                                                                   gCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGaa                 >  2:2503815/1‑150 (MQ=255)

                                                                                                                                    cAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAg                <  1:2589490/150‑1 (MQ=255)

                                                                                                                                    cAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAg                >  2:663906/1‑150 (MQ=255)

                                                                                                                                       aTCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGata             >  2:1110299/1‑150 (MQ=255)

                                                                                                                                       aTCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGata             >  2:4290287/1‑150 (MQ=255)

                                                                                                                                          aCCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTCTTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTc          >  2:306687/1‑150 (MQ=255)

                                                                                                                                           cccTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATACCTGGGCCAATGAATTAACCGAAGATATTcg         <  1:2028103/150‑1 (MQ=255)

                                                                                                                                           cccTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTcg         <  2:3316418/150‑1 (MQ=255)

                                                                                                                                            ccTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTcgc        <  1:2772882/150‑1 (MQ=255)

                                                                                                                                            ccTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTcgc        >  2:525413/1‑150 (MQ=255)

                                                                                                                                               ggTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTcgccgc     >  2:2533854/1‑150 (MQ=255)

                                                                                                                                               ggTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTcgccgc     <  2:2348925/150‑1 (MQ=255)

                                                                                                                                                  cgcgAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTTAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTCGCCGCCAg  <  1:3271840/150‑1 (MQ=255)

                                                                                                                                                  cgcgAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTCGCCGCCAg  >  2:1445742/1‑150 (MQ=255)

CCCCCATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTCGCCGCCAG  >  CP009273/2313778‑2314073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A