Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,313,924 C→A 32.0% R135S (CGC→AGC)  atoS → sensory histidine kinase in two‑component regulatory system with AtoC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,313,9240CA32.0% 394.8 / 195.1 221R135S (CGC→AGC) atoSsensory histidine kinase in two‑component regulatory system with AtoC
Reads supporting (aligned to +/- strand):  ref base C (69/81);  new base A (43/28);  total (112/109)
Fisher's exact test for biased strand distribution p-value = 4.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 223 total aligned reads displayed.

TTGCCCCCATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTCGCCGCCAG  >  CP009273/2313775‑2314073
                                                                                                                                                     |                                                                                                                                                     
ttGCCCCCATTACCGAAAAGATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTc                                                                                                                                                       <  1:1538313/150‑1 (MQ=255)
     cccATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGaa                                                                                                                                                  >  1:3142323/1‑150 (MQ=255)
     cccATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGaa                                                                                                                                                  >  2:3311682/1‑150 (MQ=255)
            ccGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGc                                                                                                                                           <  2:597368/150‑1 (MQ=255)
              gAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTATGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGt                                                                                                                                         <  2:3673109/150‑1 (MQ=255)
                aaaTATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTAc                                                                                                                                       <  1:3448403/150‑1 (MQ=255)
                 aaTATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACa                                                                                                                                      >  2:939446/1‑150 (MQ=255)
                  atatCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACaa                                                                                                                                     >  1:3302888/1‑150 (MQ=255)
                     tcactcacGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATa                                                                                                                                  <  2:792290/150‑1 (MQ=255)
                      cactcacGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCAGGTCGCGAAGTGATGCGTACAAATAc                                                                                                                                 >  1:1654043/1‑150 (MQ=255)
                        ctcacGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCCGTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATAccc                                                                                                                               >  2:4317206/1‑150 (MQ=255)
                        ctcacGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATAccc                                                                                                                               >  2:205683/1‑150 (MQ=255)
                         tcacGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATAccac                                                                                                                              <  2:4082258/150‑3 (MQ=255)
                         tcacGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATAccac                                                                                                                              <  2:1677159/150‑3 (MQ=255)
                           acGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCtt                                                                                                                            <  1:1556550/150‑1 (MQ=255)
                            cGCCGTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCttt                                                                                                                           <  2:4716599/150‑1 (MQ=255)
                             gCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTa                                                                                                                          >  1:610024/1‑150 (MQ=255)
                             gCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTa                                                                                                                          >  1:3706370/1‑150 (MQ=255)
                             gCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTa                                                                                                                          <  1:1109682/150‑1 (MQ=255)
                             gCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTa                                                                                                                          >  1:4657356/1‑150 (MQ=255)
                               cTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGt                                                                                                                        <  2:3817349/150‑1 (MQ=255)
                                ttCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGtt                                                                                                                       >  1:1031249/1‑150 (MQ=255)
                                ttCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGtt                                                                                                                       <  2:442978/150‑1 (MQ=255)
                                ttCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGtt                                                                                                                       <  1:3075823/150‑1 (MQ=255)
                                ttCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGtt                                                                                                                       >  2:1135599/1‑150 (MQ=255)
                                ttCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGtt                                                                                                                       <  1:687296/150‑1 (MQ=255)
                                 tCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGttt                                                                                                                      <  1:1843780/150‑1 (MQ=255)
                                   ccTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTAt                                                                                                                    <  1:4627393/150‑1 (MQ=255)
                                     tGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTc                                                                                                                  <  1:2946093/150‑1 (MQ=255)
                                      ggCATCGGTGCTTGTTATTACAACAAAATGCTGGATGCGATTATCAACTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTca                                                                                                                 <  1:2704210/150‑1 (MQ=255)
                                      ggCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTca                                                                                                                 >  1:2476433/1‑150 (MQ=255)
                                      ggCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTca                                                                                                                 >  1:2721376/1‑150 (MQ=255)
                                        cATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcagg                                                                                                               <  2:2233689/150‑1 (MQ=255)
                                            ggTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcagg                                                                                                           >  1:1242336/1‑150 (MQ=255)
                                              tGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcaggca                                                                                                         <  2:1437977/150‑1 (MQ=255)
                                              tGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcaggca                                                                                                         >  1:3695509/1‑150 (MQ=255)
                                              tGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTcaggcaggca                                                                                                         <  2:4419975/150‑1 (MQ=255)
                                                  ggTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTg                                                                                                     >  1:473370/1‑150 (MQ=255)
                                                   gTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTgc                                                                                                    >  2:3701929/1‑150 (MQ=255)
                                                    ttattaCAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTgcg                                                                                                   <  2:2691005/150‑1 (MQ=255)
                                                        taCAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCgcggc                                                                                               >  1:2531440/1‑150 (MQ=255)
                                                         acaacaAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCgcggcg                                                                                              <  2:2798999/150‑1 (MQ=255)
                                                           aacaaAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGat                                                                                            >  2:4579723/1‑150 (MQ=255)
                                                             caaAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGatat                                                                                          <  1:1391452/150‑1 (MQ=255)
                                                             caaAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGatat                                                                                          <  1:1391407/150‑1 (MQ=255)
                                                                aaTGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATAtttt                                                                                       >  2:4565181/1‑150 (MQ=255)
                                                                    cTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAAt                                                                                   >  1:4098340/1‑150 (MQ=255)
                                                                     tGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAAtt                                                                                  >  2:25367/1‑150 (MQ=255)
                                                                        aTGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCCGGTGCGCGGCGATATTTTGAATTCaa                                                                               >  1:894329/1‑150 (MQ=255)
                                                                          gCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATg                                                                             >  1:426147/1‑150 (MQ=255)
                                                                            gATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCt                                                                           >  1:3082736/1‑150 (MQ=255)
                                                                            gATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCt                                                                           >  2:381040/1‑150 (MQ=255)
                                                                              tAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTcc                                                                         >  2:3543031/1‑150 (MQ=255)
                                                                              tAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTcc                                                                         >  2:2558576/1‑150 (MQ=255)
                                                                              tAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGAAGATCACCCTGGTCGCGAAGTGATGAGTACAAATACCCCTTTAGTTTATTAAGGCAGGCAGGTGAGCGGCGATATTTTGAATTCAATGCTca                                                                         >  2:1773996/1‑149 (MQ=255)
                                                                               aaTCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTccc                                                                        <  2:2728812/150‑1 (MQ=255)
                                                                                aTCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTcccc                                                                       >  2:1145622/1‑150 (MQ=255)
                                                                                  cACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCAt                                                                     <  1:3582666/150‑1 (MQ=255)
                                                                                   aCCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCGTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCAtt                                                                    <  2:1934009/150‑1 (MQ=255)
                                                                                     cTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGa                                                                  >  2:725478/1‑150 (MQ=255)
                                                                                      tACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAg                                                                 >  2:3246333/1‑150 (MQ=255)
                                                                                       aCGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGc                                                                >  1:751657/1‑150 (MQ=255)
                                                                                       aCGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGc                                                                <  2:3087912/150‑1 (MQ=255)
                                                                                          cgcCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTa                                                             <  2:2586562/150‑1 (MQ=255)
                                                                                           gcCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTaa                                                            <  2:1350901/150‑1 (MQ=255)
                                                                                               tCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGt                                                        >  1:3772721/1‑150 (MQ=255)
                                                                                                cAGCGCTATATCAGAATACTGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTg                                                       <  1:4511596/150‑1 (MQ=255)
                                                                                                    gcTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAAt                                                   <  2:1964499/150‑1 (MQ=255)
                                                                                                    gcTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAAt                                                   <  1:4383593/150‑1 (MQ=255)
                                                                                                    gcTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAAt                                                   >  2:3866952/1‑150 (MQ=255)
                                                                                                    gcTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAAt                                                   >  2:1457443/1‑150 (MQ=255)
                                                                                                        tatCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTc                                               <  1:3720985/150‑1 (MQ=255)
                                                                                                        tatCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTc                                               <  1:338100/150‑1 (MQ=255)
                                                                                                         atCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATTCTCCCCATTGAGCGTAATGGTGAAATCCTCg                                              >  1:19321/1‑150 (MQ=255)
                                                                                                              aataatGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCtat                                         <  1:1667825/150‑1 (MQ=255)
                                                                                                                taatGTCGGCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTatat                                       <  2:1912370/150‑1 (MQ=255)
                                                                                                                   tGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTg                                    <  2:3772721/150‑1 (MQ=255)
                                                                                                                    gTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTgg                                   >  1:3981131/1‑150 (MQ=255)
                                                                                                                      cGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGc                                 >  2:1213838/1‑150 (MQ=255)
                                                                                                                       ggCGTTACCATTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGcc                                <  2:435344/150‑1 (MQ=255)
                                                                                                                          gTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAAt                             <  1:23617/150‑1 (MQ=255)
                                                                                                                           ttACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATg                            <  1:532059/150‑1 (MQ=255)
                                                                                                                              ccATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAAt                         >  2:4653713/1‑150 (MQ=255)
                                                                                                                               cATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAAtt                        <  1:4222621/150‑1 (MQ=255)
                                                                                                                               cATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAAtt                        >  1:3002807/1‑150 (MQ=255)
                                                                                                                                aTTGCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTa                       >  1:864784/1‑150 (MQ=255)
                                                                                                                                 ttGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTaa                      <  1:1967412/150‑1 (MQ=255)
                                                                                                                                  tGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAAc                     >  1:3234318/1‑150 (MQ=255)
                                                                                                                                   gCCGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTACTGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAAcc                    >  2:4033867/1‑150 (MQ=255)
                                                                                                                                     cGCAGATCACCCTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGa                  >  2:4737124/1‑150 (MQ=255)
                                                                                                                                      gCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCCATGAATTAACCGaa                 >  2:1360981/1‑150 (MQ=255)
                                                                                                                                      gCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGaa                 <  1:850286/150‑1 (MQ=255)
                                                                                                                                       cAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAg                >  2:2983407/1‑150 (MQ=255)
                                                                                                                                         gATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGat              <  1:342273/150‑1 (MQ=255)
                                                                                                                                         gATCACCCTGGTCGCGAAGTGATGCGTACAAAGACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGat              <  2:3987040/150‑1 (MQ=255)
                                                                                                                                          aTCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGata             <  1:205683/150‑1 (MQ=255)
                                                                                                                                            cACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATAtt           <  2:2723168/150‑1 (MQ=255)
                                                                                                                                             aCCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTc          <  2:1227212/150‑1 (MQ=255)
                                                                                                                                              cccTGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTcg         <  1:25583/150‑1 (MQ=255)
                                                                                                                                                 tGGTAGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTcgccg      >  1:2144174/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGCCCCCATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGTGCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATATCAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACAAATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTCCCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGATATTCGCCGCCAG  >  CP009273/2313775‑2314073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A