Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,759,388 C→A 18.5% N1282K (AAC→AAA)  rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,759,3880CA18.5% 764.7 / 126.2 269N1282K (AAC→AAA) rhsARhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (89/130);  new base A (21/29);  total (110/159)
Fisher's exact test for biased strand distribution p-value = 8.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 270 total aligned reads displayed.

AGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCTGGAG  >  CP009273/3759240‑3759537
                                                                                                                                                    |                                                                                                                                                     
aGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACg                                                                                                                                                      >  2:224656/1‑150 (MQ=255)
 gTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGa                                                                                                                                                     >  2:1064686/1‑150 (MQ=255)
  tATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGAt                                                                                                                                                    <  1:2491405/150‑1 (MQ=255)
     ccGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATTCTGCAAAAGCATTGACACAATCGTGGAACGATaaa                                                                                                                                                 >  2:4157810/1‑148 (MQ=255)
     ccGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACa                                                                                                                                                 <  1:1647491/150‑1 (MQ=255)
      cGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAg                                                                                                                                                <  1:30553/150‑1 (MQ=255)
       gTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGc                                                                                                                                               >  2:850608/1‑150 (MQ=255)
       gTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTACATGGCCCAAAAGCCCTGAACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGc                                                                                                                                               >  2:3477589/1‑150 (MQ=255)
        ttGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCATAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCa                                                                                                                                              <  2:2734331/150‑1 (MQ=255)
        ttGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCa                                                                                                                                              <  1:1341775/150‑1 (MQ=255)
           aaTCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATca                                                                                                                                           >  1:2245212/1‑150 (MQ=255)
             tCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATcaca                                                                                                                                         >  1:1284154/1‑150 (MQ=255)
             tCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATcaca                                                                                                                                         <  1:2406982/150‑1 (MQ=255)
                aGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAg                                                                                                                                      <  2:568642/150‑1 (MQ=255)
                    aCGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAata                                                                                                                                  >  1:2536327/1‑150 (MQ=255)
                    aCGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAata                                                                                                                                  <  1:4031155/150‑1 (MQ=255)
                       aaTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATAttt                                                                                                                               <  1:193951/150‑1 (MQ=255)
                        aTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTg                                                                                                                              >  1:1059397/1‑150 (MQ=255)
                        aTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTg                                                                                                                              <  2:1905176/150‑1 (MQ=255)
                          aCAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGAc                                                                                                                            <  1:3499574/150‑1 (MQ=255)
                          aCAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGAc                                                                                                                            <  1:2665298/150‑1 (MQ=255)
                           cAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGAct                                                                                                                           <  1:3220198/150‑1 (MQ=255)
                            aGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGActc                                                                                                                          >  1:2066258/1‑150 (MQ=255)
                            aGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGActc                                                                                                                          >  2:4126963/1‑150 (MQ=255)
                             gATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGActct                                                                                                                         <  1:3910877/150‑1 (MQ=255)
                             gATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATCTTTGActct                                                                                                                         >  2:2419523/1‑150 (MQ=255)
                               tCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGActctct                                                                                                                       <  2:1103094/150‑1 (MQ=255)
                                 ctctGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTaa                                                                                                                     <  2:321056/150‑1 (MQ=255)
                                 ctctGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTaa                                                                                                                     >  2:3665205/1‑150 (MQ=255)
                                  tctGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAat                                                                                                                    <  1:1517794/150‑1 (MQ=255)
                                    tGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAatat                                                                                                                  <  2:3447065/150‑1 (MQ=255)
                                    tGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAatat                                                                                                                  >  1:3668140/1‑150 (MQ=255)
                                     ggggTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATAtt                                                                                                                 <  2:2076005/150‑1 (MQ=255)
                                     ggggTTAGAAGTTTTTCCTAGACCATTCCCCGTGCCAAGTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATAtt                                                                                                                 <  2:3600231/150‑1 (MQ=255)
                                      gggTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTg                                                                                                                <  2:227111/150‑1 (MQ=255)
                                      gggTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTg                                                                                                                >  1:2889246/1‑150 (MQ=255)
                                        gTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGaa                                                                                                              <  2:550385/150‑1 (MQ=255)
                                         ttAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGaat                                                                                                             <  2:3763338/150‑1 (MQ=255)
                                         ttAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGaat                                                                                                             <  2:1539500/150‑1 (MQ=255)
                                          tAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGaata                                                                                                            >  1:3372083/1‑150 (MQ=255)
                                                 ttttCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCggg                                                                                                     <  1:3709710/150‑1 (MQ=255)
                                                  tttCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGa                                                                                                    <  2:3816768/150‑1 (MQ=255)
                                                   ttCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGAc                                                                                                   <  1:3603560/150‑1 (MQ=255)
                                                     ccTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTa                                                                                                 <  2:1217334/150‑1 (MQ=255)
                                                     ccTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTa                                                                                                 <  2:352401/150‑1 (MQ=255)
                                                      cTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAg                                                                                                >  1:2722142/1‑150 (MQ=255)
                                                          aCCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCAtt                                                                                            >  2:3191696/1‑150 (MQ=255)
                                                          aCCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCAtt                                                                                            >  2:1304736/1‑150 (MQ=255)
                                                          aCCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCAtt                                                                                            <  1:3811950/150‑1 (MQ=255)
                                                           ccATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTa                                                                                           <  1:3745250/150‑1 (MQ=255)
                                                           ccATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTa                                                                                           <  1:3877348/150‑1 (MQ=255)
                                                            cATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAg                                                                                          >  1:4114047/1‑150 (MQ=255)
                                                             aTTCCCCTTGCCAAGTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGA‑AATGCTGCAACAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGat                                                                                        <  2:2760467/150‑1 (MQ=255)
                                                              ttCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGat                                                                                        >  1:2647699/1‑150 (MQ=255)
                                                              ttCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGat                                                                                        <  2:1323302/150‑1 (MQ=255)
                                                               tCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGata                                                                                       <  1:1882214/150‑1 (MQ=255)
                                                                    ttGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACa                                                                                  >  1:2007166/1‑150 (MQ=255)
                                                                       ccAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATg                                                                               <  2:2375111/150‑1 (MQ=255)
                                                                       ccAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATg                                                                               <  2:2633187/150‑1 (MQ=255)
                                                                             ccATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCt                                                                         >  1:471200/1‑150 (MQ=255)
                                                                             ccATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAACAATCCGGGACTAGCATTAGATATAACAATGATAGCt                                                                         >  1:144980/1‑150 (MQ=255)
                                                                               aTGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTtc                                                                       <  2:2129915/150‑1 (MQ=255)
                                                                               aTGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTtc                                                                       >  2:5192/1‑150 (MQ=255)
                                                                                tGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTtct                                                                      >  2:750130/1‑150 (MQ=255)
                                                                                 ggCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTtctc                                                                     <  2:2881601/150‑1 (MQ=255)
                                                                                    ccAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGACTATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTg                                                                  >  2:396427/1‑150 (MQ=255)
                                                                                         aGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAAt                                                             <  2:1682978/150‑1 (MQ=255)
                                                                                             cTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTg                                                         >  1:208938/1‑150 (MQ=255)
                                                                                             cTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTg                                                         <  2:665403/150‑1 (MQ=255)
                                                                                             cTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTg                                                         <  2:665107/150‑1 (MQ=255)
                                                                                             cTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCAGTAGATATAACAATGATAGCTTCTCGTGGAAATGTTg                                                         >  1:711467/1‑150 (MQ=255)
                                                                                                 acaGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGa                                                     >  2:3317881/1‑150 (MQ=255)
                                                                                                   agcagcAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGaca                                                   >  1:2868658/1‑150 (MQ=255)
                                                                                                      agcaagcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAg                                                <  1:1764745/150‑1 (MQ=255)
                                                                                                         aagcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGa                                             >  2:1973266/1‑150 (MQ=255)
                                                                                                          agcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGAt                                            <  2:3014578/150‑1 (MQ=255)
                                                                                                            caGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATaa                                          >  1:3128034/1‑150 (MQ=255)
                                                                                                             aGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATATCAATGATAGCTTCTCGTGGTAATGTTGCAGACACAGGGATAAc                                         >  2:4288704/1‑150 (MQ=255)
                                                                                                             aGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAAc                                         <  2:3078087/150‑1 (MQ=255)
                                                                                                              gatgatAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACt                                        >  1:2186718/1‑150 (MQ=255)
                                                                                                              gatgatAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACt                                        >  1:2186708/1‑150 (MQ=255)
                                                                                                                    aaTGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCgt                                  >  1:1654841/1‑150 (MQ=255)
                                                                                                                       gctgcAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTc                               <  1:528002/150‑1 (MQ=255)
                                                                                                                       gctgcAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTc                               <  1:186642/150‑1 (MQ=255)
                                                                                                                        ctgcAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCa                              >  2:498981/1‑150 (MQ=255)
                                                                                                                          gcAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAAt                            <  2:1590514/150‑1 (MQ=255)
                                                                                                                           cAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATg                           >  1:2512616/1‑150 (MQ=255)
                                                                                                                             aaaGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGAc                         <  2:298491/150‑1 (MQ=255)
                                                                                                                             aaaGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGAc                         <  1:394167/150‑1 (MQ=255)
                                                                                                                              aaGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACa                        <  2:4030218/150‑1 (MQ=255)
                                                                                                                              aaGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACa                        <  2:2955297/150‑1 (MQ=255)
                                                                                                                                 cATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACataa                     <  1:1973589/150‑1 (MQ=255)
                                                                                                                                 cATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACataa                     <  1:3151216/150‑1 (MQ=255)
                                                                                                                                 cATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACataa                     >  2:3320977/1‑150 (MQ=255)
                                                                                                                                     gACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATaaa                 >  1:4160397/1‑150 (MQ=255)
                                                                                                                                               tggAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTc       <  2:438015/150‑1 (MQ=255)
                                                                                                                                               tggAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTc       <  1:818966/150‑1 (MQ=255)
                                                                                                                                                ggAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCt      <  2:3735576/150‑1 (MQ=255)
                                                                                                                                                  aaCGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCTgg    <  1:4295249/150‑1 (MQ=255)
                                                                                                                                                    cGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCTGGAg  >  1:2040458/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCTGGAG  >  CP009273/3759240‑3759537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A