Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,759,388 C→A 32.8% N1282K (AAC→AAA)  rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,759,3880CA32.8% 390.4 / 214.6 231N1282K (AAC→AAA) rhsARhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (58/97);  new base A (19/57);  total (77/154)
Fisher's exact test for biased strand distribution p-value = 7.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 233 total aligned reads displayed.

CAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCTG  >  CP009273/3759239‑3759534
                                                                                                                                                     |                                                                                                                                                  
cAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCGCAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAc                                                                                                                                                    <  2:4489086/150‑1 (MQ=255)
cAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAc                                                                                                                                                    <  1:2943931/150‑1 (MQ=255)
  gTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGa                                                                                                                                                  <  1:4417466/150‑1 (MQ=255)
   tATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGAt                                                                                                                                                 >  1:2020931/1‑150 (MQ=255)
   tATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGAt                                                                                                                                                 <  1:3604543/150‑1 (MQ=255)
    aTCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATa                                                                                                                                                >  2:859524/1‑150 (MQ=255)
    aTCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATa                                                                                                                                                <  2:1576362/150‑1 (MQ=255)
      ccGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACa                                                                                                                                              <  1:4003505/150‑1 (MQ=255)
       cGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAg                                                                                                                                             >  1:115845/1‑150 (MQ=255)
           gAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATc                                                                                                                                         >  1:2589163/1‑150 (MQ=255)
            aaTCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATca                                                                                                                                        <  1:2566125/150‑1 (MQ=255)
                  gTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGa                                                                                                                                  <  1:703485/150‑1 (MQ=255)
                   ttACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGaa                                                                                                                                 >  1:1141033/1‑150 (MQ=255)
                   ttACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGaa                                                                                                                                 <  1:3285355/150‑1 (MQ=255)
                    tACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAat                                                                                                                                <  2:722839/150‑1 (MQ=255)
                    tACGAATACAGATCCTCTGGGGTTAGAAGTTTGTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAat                                                                                                                                <  1:146811/150‑1 (MQ=255)
                     aCGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAata                                                                                                                               <  1:3399151/150‑1 (MQ=255)
                      cGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAatat                                                                                                                              <  2:2319583/150‑1 (MQ=255)
                      cGAATACAGATCCTCTGGGGTTAGAAGTTGTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAatat                                                                                                                              <  2:3083085/150‑1 (MQ=255)
                       gAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATAtt                                                                                                                             <  2:3993001/150‑1 (MQ=255)
                       gAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATAtt                                                                                                                             <  1:275812/150‑1 (MQ=255)
                        aaTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATAttt                                                                                                                            <  2:3465507/150‑1 (MQ=255)
                        aaTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATAttt                                                                                                                            <  1:3302944/150‑1 (MQ=255)
                        aaTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATAttt                                                                                                                            >  2:4753668/1‑150 (MQ=255)
                        aaTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATAttt                                                                                                                            <  1:1512292/150‑1 (MQ=255)
                         aTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTg                                                                                                                           <  2:1127514/150‑1 (MQ=255)
                         aTACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTg                                                                                                                           <  1:415077/150‑1 (MQ=255)
                           aCAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGAc                                                                                                                         <  1:1705536/150‑1 (MQ=255)
                            cAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGAct                                                                                                                        <  1:265317/150‑1 (MQ=255)
                            cAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACAATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGAAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAATAATATTTGAct                                                                                                                        >  2:1459260/1‑150 (MQ=255)
                               aTCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGActctc                                                                                                                     <  2:3443379/150‑1 (MQ=255)
                               aTCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGACAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGActctc                                                                                                                     <  1:4342895/150‑1 (MQ=255)
                                   tctGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAat                                                                                                                 <  1:2500861/150‑1 (MQ=255)
                                    ctGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAata                                                                                                                <  2:3168077/150‑1 (MQ=255)
                                     tGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAATAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAatat                                                                                                               <  2:3971750/150‑1 (MQ=255)
                                     tGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAatat                                                                                                               <  1:3891475/150‑1 (MQ=255)
                                      ggggTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATCCAGCATCACAAAGAATATTTGACTCTCTAATAtt                                                                                                              >  2:4394336/1‑150 (MQ=255)
                                      ggggTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATAtt                                                                                                              <  2:902318/150‑1 (MQ=255)
                                           tAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGaata                                                                                                         <  1:276641/150‑1 (MQ=255)
                                               aGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATcc                                                                                                     <  2:4112871/150‑1 (MQ=255)
                                               aGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATcc                                                                                                     <  1:4137189/150‑1 (MQ=255)
                                                 tttttCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCgg                                                                                                   <  2:3964979/150‑1 (MQ=255)
                                                    ttCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGAc                                                                                                <  1:2317134/150‑1 (MQ=255)
                                                     tCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACt                                                                                               <  2:3801341/150‑1 (MQ=255)
                                                      ccTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTa                                                                                              >  1:1073336/1‑150 (MQ=255)
                                                       cTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAg                                                                                             >  1:2713054/1‑150 (MQ=255)
                                                        tAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGc                                                                                            >  2:1672232/1‑150 (MQ=255)
                                                        tAGACCATTCCCCTTGCCAAGTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGc                                                                                            <  1:4546719/150‑1 (MQ=255)
                                                             cATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAg                                                                                       >  2:752632/1‑150 (MQ=255)
                                                               ttCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGat                                                                                     >  1:829947/1‑150 (MQ=255)
                                                               ttCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGat                                                                                     <  2:4053113/150‑1 (MQ=255)
                                                               ttCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGat                                                                                     <  2:4703548/150‑1 (MQ=255)
                                                                 ccccTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGatat                                                                                   >  1:3491462/1‑150 (MQ=255)
                                                                  cccTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGAtata                                                                                  <  2:63030/150‑1 (MQ=255)
                                                                   ccTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATaa                                                                                 >  2:3397545/1‑150 (MQ=255)
                                                                      tGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACaa                                                                              >  1:4603169/1‑150 (MQ=255)
                                                                       gCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAAt                                                                             <  2:2364880/150‑1 (MQ=255)
                                                                       gCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAAt                                                                             <  1:3115162/150‑1 (MQ=255)
                                                                         cAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGa                                                                           >  1:690472/1‑150 (MQ=255)
                                                                           aTTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATa                                                                         <  1:4494689/150‑1 (MQ=255)
                                                                             tCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGc                                                                       <  2:1467232/150‑1 (MQ=255)
                                                                              ccATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCt                                                                      <  2:2836768/150‑1 (MQ=255)
                                                                               cATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCtt                                                                     <  2:3591988/150‑1 (MQ=255)
                                                                                  ggCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTtctc                                                                  <  1:607299/150‑1 (MQ=255)
                                                                                  ggCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTtctc                                                                  <  1:3331345/150‑1 (MQ=255)
                                                                                   gCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCg                                                                 <  1:2202475/150‑1 (MQ=255)
                                                                                     ccAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTg                                                               <  1:4381485/150‑1 (MQ=255)
                                                                                      cAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTgg                                                              <  2:3813768/150‑1 (MQ=255)
                                                                                       aaaaGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGa                                                             >  2:1865596/1‑150 (MQ=255)
                                                                                        aaaGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGaa                                                            >  1:1232223/1‑150 (MQ=255)
                                                                                                gCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCa                                                    <  2:1492251/150‑1 (MQ=255)
                                                                                                 cacaGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAg                                                   <  2:26212/150‑1 (MQ=255)
                                                                                                   cagcagCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGac                                                 >  2:2749968/1‑150 (MQ=255)
                                                                                                    agcagcAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCAGTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGaca                                                >  2:4473264/1‑150 (MQ=255)
                                                                                                          aagcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGa                                          >  1:2284255/1‑150 (MQ=255)
                                                                                                          aagcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGa                                          <  1:3810937/150‑1 (MQ=255)
                                                                                                           agcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGAt                                         <  1:2996147/150‑1 (MQ=255)
                                                                                                            gcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATa                                        <  2:2996559/150‑1 (MQ=255)
                                                                                                            gcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATa                                        >  2:2874028/1‑150 (MQ=255)
                                                                                                            gcaGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATa                                        <  1:2143987/150‑1 (MQ=255)
                                                                                                             caGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATaa                                       <  1:817336/150‑1 (MQ=255)
                                                                                                              aGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAAc                                      >  2:3573378/1‑150 (MQ=255)
                                                                                                              aGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAAc                                      >  1:2461174/1‑150 (MQ=255)
                                                                                                               gatgatAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAACGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACt                                     <  2:3907773/150‑1 (MQ=255)
                                                                                                                atgatACTGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTg                                    <  2:3019433/150‑1 (MQ=255)
                                                                                                                   atAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATc                                 <  2:1795903/150‑1 (MQ=255)
                                                                                                                   atAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATc                                 >  2:1243249/1‑150 (MQ=255)
                                                                                                                   atAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATc                                 <  1:2450770/150‑1 (MQ=255)
                                                                                                                    tAATGCTGCAAAAGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCg                                <  1:39180/150‑1 (MQ=255)
                                                                                                                      aTGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCgtg                              >  1:2835956/1‑150 (MQ=255)
                                                                                                                           gcAAAAGCATTGACAAAATGGTGGAAAGATACAGAATCCAAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGCCTAGAATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAAt                         <  1:95820/150‑1 (MQ=255)
                                                                                                                            cAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATg                        >  2:849061/1‑150 (MQ=255)
                                                                                                                              aaaGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGAc                      <  2:4048359/150‑1 (MQ=255)
                                                                                                                                aGCATTGACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACat                    >  1:1857147/1‑150 (MQ=255)
                                                                                                                                   aTTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACataat                 >  1:2738724/1‑150 (MQ=255)
                                                                                                                                    ttGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACataata                <  2:378307/150‑1 (MQ=255)
                                                                                                                                      gACAAAATGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATaaa              <  2:1389976/150‑1 (MQ=255)
                                                                                                                                           aaTGGTGGAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACa         <  2:2526129/150‑1 (MQ=255)
                                                                                                                                                 ggAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCt   <  2:4244185/150‑1 (MQ=255)
                                                                                                                                                  gAAAGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCTg  <  1:1937007/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CAGTATCCGTTGAATCCAGTTACGAATACAGATCCTCTGGGGTTAGAAGTTTTTCCTAGACCATTCCCCTTGCCAATTCCATGGCCCAAAAGCCCTGCACAGCAGCAAGCAGATGATAATGCTGCAAAAGCATTGACAAAATGGTGGAACGATACAGCATCACAAAGAATATTTGACTCTCTAATATTGAATAATCCGGGACTAGCATTAGATATAACAATGATAGCTTCTCGTGGAAATGTTGCAGACACAGGGATAACTGATCGTGTCAATGACATAATAAATGACAGATTCTG  >  CP009273/3759239‑3759534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A