Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 217,887 C→T W74* (TGG→TGA)  metI ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273217,8870CT100.0% 577.1 / NA 174W74* (TGG→TGA) metIDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (91/83);  total (91/83)

Only 100 of 179 total aligned reads displayed.

GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAA  >  CP009273/217741‑218032
                                                                                                                                                  |                                                                                                                                                 
 aaGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATa                                                                                                                                               >  1:920406/1‑150 (MQ=255)
    cTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaa                                                                                                                                            <  1:1505737/150‑1 (MQ=255)
        aaTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaa                                                                                                                                        >  1:387186/1‑150 (MQ=255)
        aaTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaa                                                                                                                                        <  2:1575660/150‑1 (MQ=255)
         aTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaag                                                                                                                                       <  1:2503414/150‑1 (MQ=255)
         aTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaag                                                                                                                                       <  1:2503268/150‑1 (MQ=255)
          ttAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGa                                                                                                                                      >  2:103832/1‑150 (MQ=255)
                 ggTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGaa                                                                                                                               <  2:2917552/150‑1 (MQ=255)
                  gTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAAc                                                                                                                              >  1:849990/1‑150 (MQ=255)
                  gTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAAc                                                                                                                              <  1:638375/150‑1 (MQ=255)
                   ttGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACg                                                                                                                             <  2:795480/150‑1 (MQ=255)
                       gATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGAt                                                                                                                         <  1:1189827/150‑1 (MQ=255)
                        aTCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATg                                                                                                                        >  2:64202/1‑150 (MQ=255)
                          ctcCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGa                                                                                                                      >  1:1259607/1‑150 (MQ=255)
                          ctcCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACAAATCGATGTACAGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGa                                                                                                                      <  1:1377895/150‑1 (MQ=255)
                          ctcCAGCAGAGAGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCATCGGTTAACGGAACAATCGCTGCCTGCTAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGa                                                                                                                      <  1:1453416/150‑1 (MQ=255)
                              agcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACgg                                                                                                                  >  2:2178586/1‑150 (MQ=255)
                               gcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGa                                                                                                                 >  2:2142944/1‑150 (MQ=255)
                                 agagCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGaaa                                                                                                               >  2:1589824/1‑150 (MQ=255)
                                 agagCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGaaa                                                                                                               >  2:2962315/1‑150 (MQ=255)
                                      gTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGACCGGAAAATGtt                                                                                                          >  1:2418116/1‑150 (MQ=255)
                                      gTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                          <  1:2132283/150‑1 (MQ=255)
                                      gTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                          <  1:2141688/150‑1 (MQ=255)
                                      gTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                          <  1:230820/150‑1 (MQ=255)
                                      gTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                          >  1:3256/1‑150 (MQ=255)
                                        tctcGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTca                                                                                                        >  2:3124786/1‑150 (MQ=255)
                                          tcGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTcacc                                                                                                      <  2:3039049/150‑2 (MQ=255)
                                          tcGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTcacc                                                                                                      <  1:3789040/150‑2 (MQ=255)
                                           cGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTcacca                                                                                                     >  2:3209539/1‑148 (MQ=255)
                                             aCCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATc                                                                                                   <  2:3360798/150‑1 (MQ=255)
                                              ccATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCg                                                                                                  >  2:1362599/1‑150 (MQ=255)
                                              ccATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCg                                                                                                  <  1:681455/150‑1 (MQ=255)
                                                aTACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCa                                                                                                >  2:432601/1‑150 (MQ=255)
                                                   cGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGaa                                                                                             >  2:1447726/1‑150 (MQ=255)
                                                    gggCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGACCGGAAAATGTTCACCATCGCAGaaa                                                                                            >  2:1778272/1‑150 (MQ=255)
                                                    gggCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGaaa                                                                                            >  2:1778065/1‑150 (MQ=255)
                                                    gggCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGaaa                                                                                            >  1:3169028/1‑150 (MQ=255)
                                                        aaTAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGt                                                                                        >  2:2536317/1‑150 (MQ=255)
                                                         aTAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTa                                                                                       <  2:2429367/150‑1 (MQ=255)
                                                            aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGa                                                                                    >  1:2898802/1‑150 (MQ=255)
                                                               ggTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATAc                                                                                 <  2:153670/150‑1 (MQ=255)
                                                                 tgctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAg                                                                               >  2:1912866/1‑150 (MQ=255)
                                                                  gctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGc                                                                              >  2:2675946/1‑150 (MQ=255)
                                                                     gcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTc                                                                           <  1:3337100/150‑1 (MQ=255)
                                                                           aCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTa                                                                     <  2:2721786/150‑1 (MQ=255)
                                                                            cGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAg                                                                    >  2:674162/1‑150 (MQ=255)
                                                                                tAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaat                                                                >  2:2280142/1‑150 (MQ=255)
                                                                                 aaCGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaata                                                               >  2:1203878/1‑150 (MQ=255)
                                                                                 aaCGCAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaata                                                               >  2:3037511/1‑150 (MQ=255)
                                                                                  aCGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataa                                                              <  1:662449/150‑1 (MQ=255)
                                                                                   cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataat                                                             <  2:1556632/150‑1 (MQ=255)
                                                                                   cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataat                                                             >  2:1984672/1‑150 (MQ=255)
                                                                                   cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataat                                                             <  2:1053451/150‑1 (MQ=255)
                                                                                    ggAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATCCAGCTTCGCGTTAGCAATAAtt                                                            >  2:3790829/1‑150 (MQ=255)
                                                                                         aaTCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGccc                                                       <  1:2798077/150‑1 (MQ=255)
                                                                                         aaTCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGccc                                                       >  2:3716157/1‑150 (MQ=255)
                                                                                          aTCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGcccc                                                      <  2:2095583/150‑1 (MQ=255)
                                                                                          aTCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGcccc                                                      >  2:2986176/1‑150 (MQ=255)
                                                                                          aTCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGcccc                                                      <  1:1033991/150‑1 (MQ=255)
                                                                                             gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGa                                                   <  2:3090360/150‑1 (MQ=255)
                                                                                               tgcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAcg                                                 >  2:1283911/1‑150 (MQ=255)
                                                                                                 cctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAcgcg                                               >  1:341357/1‑150 (MQ=255)
                                                                                                  ctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                              >  2:1649262/1‑150 (MQ=255)
                                                                                                  ctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                              >  2:2361904/1‑150 (MQ=255)
                                                                                                  ctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                              >  1:2617164/1‑150 (MQ=255)
                                                                                                   tgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTACACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTg                                             >  1:891014/1‑150 (MQ=255)
                                                                                                     cAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGAc                                           <  1:543935/150‑1 (MQ=255)
                                                                                                      aaaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACa                                          >  2:1469526/1‑150 (MQ=255)
                                                                                                      aaaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACa                                          >  2:1180853/1‑150 (MQ=255)
                                                                                                           aaTCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAg                                     <  2:354385/150‑1 (MQ=255)
                                                                                                           aaTCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAg                                     <  2:354300/150‑1 (MQ=255)
                                                                                                            aTCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGc                                    >  1:2109319/1‑150 (MQ=255)
                                                                                                                 tGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAAc                               <  2:2268392/150‑1 (MQ=255)
                                                                                                                 tGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAAc                               >  2:2439980/1‑150 (MQ=255)
                                                                                                                    aCCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGcc                            >  2:2622874/1‑150 (MQ=255)
                                                                                                                      cGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCaa                          <  1:103832/150‑1 (MQ=255)
                                                                                                                      cGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCaa                          <  1:1253506/150‑1 (MQ=255)
                                                                                                                       gACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAAc                         >  2:298735/1‑150 (MQ=255)
                                                                                                                       gACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAAc                         >  2:3433628/1‑150 (MQ=255)
                                                                                                                         cAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCg                       >  1:2000674/1‑150 (MQ=255)
                                                                                                                           aTAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGc                     >  2:3567141/1‑150 (MQ=255)
                                                                                                                           aTAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGc                     <  1:2157214/150‑1 (MQ=255)
                                                                                                                            tAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCa                    <  2:849990/150‑1 (MQ=255)
                                                                                                                                   ggTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAAt             <  2:586334/150‑1 (MQ=255)
                                                                                                                                     tAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca           <  2:1259607/150‑1 (MQ=255)
                                                                                                                                     tAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca           >  1:3580426/1‑150 (MQ=255)
                                                                                                                                     tAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca           <  1:1589438/150‑1 (MQ=255)
                                                                                                                                      aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATcac          <  1:1106023/150‑1 (MQ=255)
                                                                                                                                      aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATcac          >  2:2158522/1‑150 (MQ=255)
                                                                                                                                      aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATcac          <  2:666422/150‑1 (MQ=255)
                                                                                                                                       aaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATcaca         >  1:2693711/1‑150 (MQ=255)
                                                                                                                                       aaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATcaca         <  2:37788/150‑1 (MQ=255)
                                                                                                                                        aCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACaa        <  1:3769614/150‑1 (MQ=255)
                                                                                                                                         cGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACaaa       >  1:1632499/1‑150 (MQ=255)
                                                                                                                                         cGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACaaa       <  2:294225/150‑1 (MQ=255)
                                                                                                                                          ggAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAg      >  2:695511/1‑150 (MQ=255)
                                                                                                                                          ggAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAg      >  1:3696015/1‑150 (MQ=255)
                                                                                                                                             atcattCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCa   >  1:1325290/1‑150 (MQ=255)
                                                                                                                                             atcattCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCa   <  1:2831028/150‑1 (MQ=255)
                                                                                                                                              tcattcatACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaa  >  2:1281496/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAA  >  CP009273/217741‑218032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAA  >  CP009273/217738‑218036
                                                                                                                                                     |                                                                                                                                                     
CGGGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATT                                                                                                                                                       >  SRR19973061.2502183/1‑150 (MQ=60)
 GGGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTC                                                                                                                                                      >  SRR19973061.1056160/1‑150 (MQ=60)
   GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGACTCATTCAT                                                                                                                                                    >  SRR19973061.2100852/1‑150 (MQ=60)
    AAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATA                                                                                                                                                   >  SRR19973061.922760/1‑150 (MQ=60)
       CTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAA                                                                                                                                                <  SRR19973061.1509506/150‑1 (MQ=60)
          CAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCA                                                                                                                                             <  SRR19973061.552166/150‑1 (MQ=60)
           AATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAA                                                                                                                                            >  SRR19973061.388243/1‑150 (MQ=60)
            ATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAG                                                                                                                                           <  SRR19973061.2509789/150‑1 (MQ=60)
            ATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAG                                                                                                                                           <  SRR19973061.2509643/150‑1 (MQ=60)
                     GTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAAC                                                                                                                                  <  SRR19973061.639997/150‑1 (MQ=60)
                     GTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAAC                                                                                                                                  >  SRR19973061.852209/1‑150 (MQ=60)
                      TTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACG                                                                                                                                 <  SRR19973061.3342510/150‑1 (MQ=60)
                      TTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACG                                                                                                                                 <  SRR19973061.3039640/150‑1 (MQ=60)
                          GATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGAT                                                                                                                             <  SRR19973061.1192825/150‑1 (MQ=60)
                             CTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGA                                                                                                                          >  SRR19973061.1262719/1‑150 (MQ=60)
                             CTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGA                                                                                                                          >  SRR19973061.1264127/1‑150 (MQ=60)
                             CTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACAAATCGATGTACAGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGA                                                                                                                          <  SRR19973061.1381378/150‑1 (MQ=60)
                             CTCCAGCAGAGAGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCATCGGTTAACGGAACAATCGCTGCCTGCTAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGA                                                                                                                          <  SRR19973061.1457084/150‑1 (MQ=60)
                                         GTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGACCGGAAAATGTT                                                                                                              >  SRR19973061.2424291/1‑150 (MQ=60)
                                         GTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTT                                                                                                              <  SRR19973061.231492/150‑1 (MQ=60)
                                         GTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTT                                                                                                              <  SRR19973061.2147066/150‑1 (MQ=60)
                                         GTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTT                                                                                                              >  SRR19973061.3264/1‑150 (MQ=60)
                                         GTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTT                                                                                                              <  SRR19973061.2137640/150‑1 (MQ=60)
                                             TCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACC                                                                                                          <  SRR19973061.3798696/150‑1 (MQ=60)
                                                ACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATC                                                                                                       <  SRR19973061.2329173/150‑1 (MQ=60)
                                                 CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCG                                                                                                      <  SRR19973061.683222/150‑1 (MQ=60)
                                                 CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCG                                                                                                      >  SRR19973061.122143/1‑150 (MQ=60)
                                                      CGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAA                                                                                                 <  SRR19973061.888805/150‑1 (MQ=60)
                                                       GGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAA                                                                                                >  SRR19973061.3177157/1‑150 (MQ=60)
                                                               AACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGA                                                                                        >  SRR19973061.2906240/1‑150 (MQ=60)
                                                               AACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGA                                                                                        >  SRR19973061.668144/1‑150 (MQ=60)
                                                                        GCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTC                                                                               <  SRR19973061.3345695/150‑1 (MQ=60)
                                                                         CACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCG                                                                              <  SRR19973061.369001/150‑1 (MQ=60)
                                                                                   TAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAAT                                                                    <  SRR19973061.905557/150‑1 (MQ=60)
                                                                                     ACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAA                                                                  <  SRR19973061.664151/150‑1 (MQ=60)
                                                                                         AACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTG                                                              >  SRR19973061.2523517/1‑150 (MQ=60)
                                                                                            AATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCC                                                           <  SRR19973061.2805267/150‑1 (MQ=60)
                                                                                             ATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCC                                                          <  SRR19973061.1036641/150‑1 (MQ=60)
                                                                                                    CCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCG                                                   >  SRR19973061.342262/1‑150 (MQ=60)
                                                                                                    CCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAAAGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCG                                                   <  SRR19973061.3781066/150‑1 (MQ=60)
                                                                                                     CTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGT                                                  >  SRR19973061.2623885/1‑150 (MQ=60)
                                                                                                     CTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGT                                                  >  SRR19973061.2623899/1‑150 (MQ=60)
                                                                                                      TGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTACACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTG                                                 >  SRR19973061.893315/1‑150 (MQ=60)
                                                                                                        CAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGAC                                               <  SRR19973061.545366/150‑1 (MQ=60)
                                                                                                               ATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGC                                        >  SRR19973061.2114613/1‑150 (MQ=60)
                                                                                                                     GTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACG                                  >  SRR19973061.147041/1‑150 (MQ=60)
                                                                                                                      TACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGC                                 >  SRR19973061.47385/1‑150 (MQ=60)
                                                                                                                       ACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCC                                <  SRR19973061.3217787/150‑1 (MQ=60)
                                                                                                                         CGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAA                              <  SRR19973061.104178/150‑1 (MQ=60)
                                                                                                                         CGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAA                              <  SRR19973061.1256611/150‑1 (MQ=60)
                                                                                                                           ACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACC                            <  SRR19973061.1177261/150‑1 (MQ=60)
                                                                                                                            CAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCG                           >  SRR19973061.2005683/1‑150 (MQ=60)
                                                                                                                              ATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGC                         <  SRR19973061.1366035/150‑1 (MQ=60)
                                                                                                                              ATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGC                         >  SRR19973061.2129968/1‑150 (MQ=60)
                                                                                                                              ATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGC                         <  SRR19973061.2162633/150‑1 (MQ=60)
                                                                                                                              ATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTCCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGC                         >  SRR19973061.1068208/1‑150 (MQ=60)
                                                                                                                               TAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCA                        <  SRR19973061.2803919/150‑1 (MQ=60)
                                                                                                                                   GCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACC                    <  SRR19973061.3583274/150‑1 (MQ=60)
                                                                                                                                   GCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACC                    <  SRR19973061.1451380/150‑1 (MQ=60)
                                                                                                                                      GGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATTCAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAAT                 <  SRR19973061.2184060/150‑1 (MQ=60)
                                                                                                                                      GGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAAT                 <  SRR19973061.982380/150‑1 (MQ=60)
                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               <  SRR19973061.1593386/150‑1 (MQ=60)
                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               <  SRR19973061.1593774/150‑1 (MQ=60)
                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               >  SRR19973061.1690764/1‑150 (MQ=60)
                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               >  SRR19973061.3725932/1‑150 (MQ=60)
                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               >  SRR19973061.3589605/1‑150 (MQ=60)
                                                                                                                                         AAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCAC              <  SRR19973061.1108852/150‑1 (MQ=60)
                                                                                                                                          AACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACA             >  SRR19973061.2700634/1‑150 (MQ=60)
                                                                                                                                           ACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAA            <  SRR19973061.3779241/150‑1 (MQ=60)
                                                                                                                                            CGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAA           >  SRR19973061.1636584/1‑150 (MQ=60)
                                                                                                                                             GGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTCCGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAG          >  SRR19973061.2428632/1‑150 (MQ=60)
                                                                                                                                             GGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAG          <  SRR19973061.2148324/150‑1 (MQ=60)
                                                                                                                                             GGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAG          >  SRR19973061.3705461/1‑150 (MQ=60)
                                                                                                                                             GGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAG          >  SRR19973061.2848426/1‑150 (MQ=60)
                                                                                                                                                ATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCA       <  SRR19973061.2838278/150‑1 (MQ=60)
                                                                                                                                                ATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCA       <  SRR19973061.64442/150‑1 (MQ=60)
                                                                                                                                                ATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGATAGCAATAATTTGCCCCGGACGCGTGACATAAATCAGAACGCCAACCGGCAGACCAATCACAAATCCA       >  SRR19973061.1328622/1‑150 (MQ=60)
                                                                                                                                                     TCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACACAGCCAAAAAA  >  SRR19973061.380116/1‑150 (MQ=60)
                                                                                                                                                     TCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAA  >  SRR19973061.380123/1‑150 (MQ=60)
                                                                                                                                                     TCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAA  >  SRR19973061.2573144/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGGGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAA  >  CP009273/217738‑218036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑