Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F247 I0 R1	1496	299.8	9582018	97.8%	9371213	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	217,887	C→T	86.1%	W74* (TGG→TGA)	metI ←	DL‑methionine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	217,887	0	C	T	86.1%	731.1 / 101.4	267	W74* (TGG→TGA)	metI	DL‑methionine transporter subunit
Reads supporting (aligned to +/- strand): ref base C (21/16); new base T (116/113); total (138/129)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 268 total aligned reads displayed.

GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCA  >  CP009273/217741‑218031

gAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCa                                                                                                                                                <  1:3548385/149‑1 (MQ=255)

gAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCa                                                                                                                                                <  1:2423827/149‑1 (MQ=255)

  aGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATa                                                                                                                                              >  2:2060027/1‑149 (MQ=255)

  aGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATa                                                                                                                                              >  2:2520425/1‑149 (MQ=255)

  aGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATa                                                                                                                                              <  1:3585108/149‑1 (MQ=255)

  aGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATa                                                                                                                                              >  2:1314003/1‑149 (MQ=255)

    cTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAca                                                                                                                                            >  2:2799189/1‑149 (MQ=255)

    cTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAca                                                                                                                                            >  2:641839/1‑149 (MQ=255)

     ttCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaa                                                                                                                                           >  2:3600877/1‑149 (MQ=255)

       cAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagc                                                                                                                                         <  2:4140089/149‑1 (MQ=255)

       cAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagc                                                                                                                                         <  2:2017268/149‑1 (MQ=255)

        aaTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGTCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagca                                                                                                                                        <  2:2541089/149‑1 (MQ=255)

        aaTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagca                                                                                                                                        >  2:4081348/1‑149 (MQ=255)

        aaTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagca                                                                                                                                        <  1:1996451/149‑1 (MQ=255)

         aTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaa                                                                                                                                       <  1:1683920/149‑1 (MQ=255)

         aTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaa                                                                                                                                       <  1:1684407/149‑1 (MQ=255)

         aTTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaa                                                                                                                                       <  2:2016087/149‑1 (MQ=255)

          ttAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATAcaagcaag                                                                                                                                      >  1:4310913/1‑149 (MQ=255)

           tAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGa                                                                                                                                     <  2:315780/149‑1 (MQ=255)

            aaCCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGAt                                                                                                                                    >  2:680559/1‑149 (MQ=255)

            aaCCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGAt                                                                                                                                    >  2:175908/1‑149 (MQ=255)

             aCCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATa                                                                                                                                   >  2:4514600/1‑149 (MQ=255)

              cccGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATaa                                                                                                                                  <  1:1512638/149‑1 (MQ=255)

              cccGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATaa                                                                                                                                  >  2:1425541/1‑149 (MQ=255)

                cGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATg                                                                                                                                >  1:2779858/1‑149 (MQ=255)

                 ggTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGa                                                                                                                               >  1:2458957/1‑149 (MQ=255)

                  gTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGaa                                                                                                                              <  2:2974903/149‑1 (MQ=255)

                     gggATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACgg                                                                                                                           <  1:681434/149‑1 (MQ=255)

                               gcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACgg                                                                                                                 <  1:2875597/149‑1 (MQ=255)

                               gcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACgg                                                                                                                 <  2:1625731/149‑1 (MQ=255)

                               gcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACgg                                                                                                                 >  1:1783283/1‑149 (MQ=255)

                               gcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACgg                                                                                                                 <  2:710947/149‑1 (MQ=255)

                                  gagCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGaaa                                                                                                              >  1:3870175/1‑149 (MQ=255)

                                   agCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGaaaa                                                                                                             >  1:1291068/1‑149 (MQ=255)

                                      gTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGt                                                                                                          >  2:3616480/1‑149 (MQ=255)

                                       ttCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                         >  2:3344268/1‑149 (MQ=255)

                                       ttCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                         <  1:3704796/149‑1 (MQ=255)

                                        tctcGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTc                                                                                                        <  1:3471530/149‑1 (MQ=255)

                                            gACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTcacca                                                                                                    >  1:3292410/1‑147 (MQ=255)

                                            gACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTcacca                                                                                                    <  2:3690209/149‑3 (MQ=255)

                                             aCCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCAt                                                                                                   >  1:1063444/1‑149 (MQ=255)

                                              ccATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGACGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATc                                                                                                  <  1:3606217/149‑1 (MQ=255)

                                               cATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCg                                                                                                 >  1:596981/1‑149 (MQ=255)

                                                 tACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCa                                                                                               <  1:4512513/149‑1 (MQ=255)

                                                 tACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCa                                                                                               >  2:1282186/1‑149 (MQ=255)

                                                    gggCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGaa                                                                                            <  2:3914456/149‑1 (MQ=255)

                                                      gCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAAc                                                                                          <  2:4573225/149‑1 (MQ=255)

                                                         aTAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGt                                                                                       <  1:4693823/149‑1 (MQ=255)

                                                          tAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTa                                                                                      <  2:4062866/149‑1 (MQ=255)

                                                            aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGACCGGAAAATGTTCACCATCGCAGAAACGGTCCg                                                                                    >  2:3404103/1‑149 (MQ=255)

                                                            aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACg                                                                                    >  1:3984626/1‑149 (MQ=255)

                                                               ggTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATa                                                                                 <  1:3090413/149‑1 (MQ=255)

                                                                   ctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGc                                                                             <  2:2867227/149‑1 (MQ=255)

                                                                      cACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTc                                                                          <  2:1493940/149‑1 (MQ=255)

                                                                      cACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTc                                                                          >  2:2919862/1‑149 (MQ=255)

                                                                       aCCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATTGCAGAAACGGTACGATACAGCTTcg                                                                         >  2:3452262/1‑149 (MQ=255)

                                                                       aCCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTcg                                                                         >  2:677403/1‑149 (MQ=255)

                                                                          aaCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGt                                                                      <  1:4400125/149‑1 (MQ=255)

                                                                          aaCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGt                                                                      >  1:2523489/1‑149 (MQ=255)

                                                                              gTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGc                                                                  >  1:408324/1‑149 (MQ=255)

                                                                               ttAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCa                                                                 >  1:842304/1‑149 (MQ=255)

                                                                               ttAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCa                                                                 <  2:3063942/149‑1 (MQ=255)

                                                                                tAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaa                                                                <  1:2185127/149‑1 (MQ=255)

                                                                                 aaCGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaat                                                               <  2:1881786/149‑1 (MQ=255)

                                                                                   cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataa                                                             >  1:882121/1‑149 (MQ=255)

                                                                                   cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataa                                                             <  2:2830305/149‑1 (MQ=255)

                                                                                   cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataa                                                             <  1:101493/149‑1 (MQ=255)

                                                                                    ggAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataat                                                            >  2:4405337/1‑149 (MQ=255)

                                                                                     gAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAAtt                                                           >  1:88583/1‑149 (MQ=255)

                                                                                       aCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTg                                                         >  1:3261844/1‑149 (MQ=255)

                                                                                       aCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTg                                                         <  2:570491/149‑1 (MQ=255)

                                                                                          aTCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGccc                                                      >  2:2199591/1‑149 (MQ=255)

                                                                                               tgcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAc                                                 <  1:1314003/149‑1 (MQ=255)

                                                                                                gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAcg                                                >  2:1152519/1‑149 (MQ=255)

                                                                                                   tgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                             <  2:584982/149‑1 (MQ=255)

                                                                                                      aaaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGAc                                          >  1:1376816/1‑149 (MQ=255)

                                                                                                      aaaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGAc                                          >  1:1867099/1‑149 (MQ=255)

                                                                                                       aaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCACACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACa                                         >  2:3962086/1‑149 (MQ=255)

                                                                                                        aCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACAt                                        >  1:1735414/1‑149 (MQ=255)

                                                                                                          cAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATaa                                      <  2:91139/149‑1 (MQ=255)

                                                                                                           aaTCGATGTACCGACTATATCGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATaaa                                     <  1:2212813/149‑1 (MQ=255)

                                                                                                            aTCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTAGCCCCGGACGCGTGACATAAAg                                    <  2:2203335/149‑1 (MQ=255)

                                                                                                             tCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGc                                   >  2:3876635/1‑149 (MQ=255)

                                                                                                               gATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAg                                 <  1:1923959/149‑1 (MQ=255)

                                                                                                                aTGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGa                                <  2:4307148/149‑1 (MQ=255)

                                                                                                                   tACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACg                             >  1:2160780/1‑149 (MQ=255)

                                                                                                                      cGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCa                          <  1:4477087/149‑1 (MQ=255)

                                                                                                                         cAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAAcc                       <  1:3368925/149‑1 (MQ=255)

                                                                                                                          aaTAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCg                      >  2:1405613/1‑149 (MQ=255)

                                                                                                                          aaTAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCg                      >  1:3539931/1‑149 (MQ=255)

                                                                                                                              aCGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGATTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAg                  >  2:1407047/1‑149 (MQ=255)

                                                                                                                                    gTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAAt            <  1:1224610/149‑1 (MQ=255)

                                                                                                                                      aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca          >  2:3471984/1‑149 (MQ=255)

                                                                                                                                      aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca          >  2:4279036/1‑149 (MQ=255)

                                                                                                                                      aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca          >  1:2640828/1‑149 (MQ=255)

                                                                                                                                      aaaCGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGATTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGACAACCGGCAGACCAATca          >  2:4706953/1‑149 (MQ=255)

                                                                                                                                           gAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAg     >  2:1579872/1‑149 (MQ=255)

                                                                                                                                            aaTCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGc    >  2:265565/1‑149 (MQ=255)

                                                                                                                                            aaTCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGc    <  1:1588070/149‑1 (MQ=255)

                                                                                                                                            aaTCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGc    >  1:4421649/1‑149 (MQ=255)

GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCA  >  CP009273/217741‑218031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAA  >  CP009273/217738‑218036

CGGGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATC                                                                                                                                                       <  SRR19973071.402757/150‑1 (MQ=60)

 GGGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTC                                                                                                                                                      <  SRR19973071.2516061/150‑1 (MQ=60)

  GGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCA                                                                                                                                                     >  SRR19973071.4364010/1‑150 (MQ=60)

   GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCAT                                                                                                                                                    <  SRR19973071.3560949/150‑1 (MQ=60)

   GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCAT                                                                                                                                                    <  SRR19973071.2432588/150‑1 (MQ=60)

   GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCAT                                                                                                                                                    <  SRR19973071.3560770/150‑1 (MQ=60)

   GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCAT                                                                                                                                                    <  SRR19973071.3560747/150‑1 (MQ=60)

   GAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGAAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCAT                                                                                                                                                    <  SRR19973071.3385435/150‑1 (MQ=60)

     AGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAC                                                                                                                                                  <  SRR19973071.3597538/150‑1 (MQ=60)

     AGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAC                                                                                                                                                  <  SRR19973071.3597554/150‑1 (MQ=60)

      GCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACA                                                                                                                                                 <  SRR19973071.4629887/150‑1 (MQ=60)

        TTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAG                                                                                                                                               <  SRR19973071.742969/150‑1 (MQ=60)

           AATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAA                                                                                                                                            <  SRR19973071.2003647/150‑1 (MQ=60)

           AATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAA                                                                                                                                            <  SRR19973071.2003633/150‑1 (MQ=60)

            ATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAG                                                                                                                                           <  SRR19973071.1690132/150‑1 (MQ=60)

            ATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAG                                                                                                                                           <  SRR19973071.1690587/150‑1 (MQ=60)

            ATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAG                                                                                                                                           <  SRR19973071.1690170/150‑1 (MQ=60)

            ATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAG                                                                                                                                           <  SRR19973071.1690620/150‑1 (MQ=60)

            ATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAG                                                                                                                                           >  SRR19973071.4326158/1‑150 (MQ=60)

             TTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGA                                                                                                                                          >  SRR19973071.1066915/1‑150 (MQ=60)

              TAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGAT                                                                                                                                         >  SRR19973071.1922690/1‑150 (MQ=60)

                 CCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAAT                                                                                                                                      <  SRR19973071.1518102/150‑1 (MQ=60)

                  CCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATG                                                                                                                                     >  SRR19973071.2789631/1‑150 (MQ=60)

                   CGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGA                                                                                                                                    >  SRR19973071.2467900/1‑150 (MQ=60)

                    GGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAA                                                                                                                                   <  SRR19973071.1335578/150‑1 (MQ=60)

                     GTTGGGATCTCCAGCAGAGCGTTCTCGAACATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAAC                                                                                                                                  <  SRR19973071.2854724/150‑1 (MQ=60)

                        GGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGG                                                                                                                               <  SRR19973071.683871/150‑1 (MQ=60)

                           ATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATG                                                                                                                            <  SRR19973071.4420981/150‑1 (MQ=60)

                                CAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACG                                                                                                                       >  SRR19973071.337707/1‑150 (MQ=60)

                                 AGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGG                                                                                                                      >  SRR19973071.2493567/1‑150 (MQ=60)

                                 AGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGG                                                                                                                      >  SRR19973071.1789933/1‑150 (MQ=60)

                                  GCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGA                                                                                                                     <  SRR19973071.2885853/150‑1 (MQ=60)

                                    AGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAA                                                                                                                   >  SRR19973071.3884592/1‑150 (MQ=60)

                                    AGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAA                                                                                                                   >  SRR19973071.3883872/1‑150 (MQ=60)

                                     GAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAA                                                                                                                  >  SRR19973071.1295769/1‑150 (MQ=60)

                                          TTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTC                                                                                                             <  SRR19973071.3717917/150‑1 (MQ=60)

                                          TTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTC                                                                                                             <  SRR19973071.3717466/150‑1 (MQ=60)

                                           TCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCA                                                                                                            <  SRR19973071.3483764/150‑1 (MQ=60)

                                             TCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACC                                                                                                          <  SRR19973071.1986113/150‑1 (MQ=60)

                                              CGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCA                                                                                                         >  SRR19973071.1415374/1‑150 (MQ=60)

                                              CGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCA                                                                                                         >  SRR19973071.3304078/1‑150 (MQ=60)

                                               GACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCAT                                                                                                        >  SRR19973071.1067244/1‑150 (MQ=60)

                                               GACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCAT                                                                                                        >  SRR19973071.2869070/1‑150 (MQ=60)

                                                 CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCG                                                                                                      >  SRR19973071.599203/1‑150 (MQ=60)

                                                 CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCG                                                                                                      >  SRR19973071.4461136/1‑150 (MQ=60)

                                                 CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGACGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCG                                                                                                      <  SRR19973071.3618809/150‑1 (MQ=60)

                                                  CATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGC                                                                                                     >  SRR19973071.1313495/1‑150 (MQ=60)

                                                    TACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAG                                                                                                   <  SRR19973071.4528553/150‑1 (MQ=60)

                                                    TACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAG                                                                                                   <  SRR19973071.4528566/150‑1 (MQ=60)

                                                      CGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAA                                                                                                 <  SRR19973071.3601807/150‑1 (MQ=60)

                                                          CAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGG                                                                                             >  SRR19973071.1592602/1‑150 (MQ=60)

                                                           AATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATTAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGT                                                                                            >  SRR19973071.1454263/1‑150 (MQ=60)

                                                            ATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTA                                                                                           <  SRR19973071.4710494/150‑1 (MQ=60)

                                                            ATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTA                                                                                           <  SRR19973071.612473/150‑1 (MQ=60)

                                                            ATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTA                                                                                           <  SRR19973071.4710530/150‑1 (MQ=60)

                                                              AAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACG                                                                                         <  SRR19973071.344695/150‑1 (MQ=60)

                                                              AAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACG                                                                                         <  SRR19973071.227509/150‑1 (MQ=60)

                                                              AAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACG                                                                                         >  SRR19973071.1838673/1‑150 (MQ=60)

                                                              AAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACG                                                                                         >  SRR19973071.3998568/1‑150 (MQ=60)

                                                              AAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACG                                                                                         >  SRR19973071.3719182/1‑150 (MQ=60)

                                                                ACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGAT                                                                                       <  SRR19973071.2712898/150‑1 (MQ=60)

                                                                  GGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATAC                                                                                     <  SRR19973071.3101328/150‑1 (MQ=60)

                                                                  GGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATAC                                                                                     <  SRR19973071.2454223/150‑1 (MQ=60)

                                                                   GTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACA                                                                                    <  SRR19973071.367334/150‑1 (MQ=60)

                                                                   GTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACA                                                                                    <  SRR19973071.854783/150‑1 (MQ=60)

                                                                   GTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACA                                                                                    <  SRR19973071.3329941/150‑1 (MQ=60)

                                                                        GCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTC                                                                               <  SRR19973071.292426/150‑1 (MQ=60)

                                                                        GCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTC                                                                               >  SRR19973071.290306/1‑150 (MQ=60)

                                                                          ACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGC                                                                             >  SRR19973071.2018227/1‑150 (MQ=60)

                                                                           CCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCG                                                                            <  SRR19973071.3104759/150‑1 (MQ=60)

                                                                            CAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGT                                                                           >  SRR19973071.2532671/1‑150 (MQ=60)

                                                                             AACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTT                                                                          <  SRR19973071.4415576/150‑1 (MQ=60)

                                                                               CGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAG                                                                        >  SRR19973071.218650/1‑150 (MQ=60)

                                                                                GGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGC                                                                       >  SRR19973071.3267281/1‑150 (MQ=60)

                                                                                GGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGC                                                                       >  SRR19973071.409645/1‑150 (MQ=60)

                                                                                GGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGC                                                                       <  SRR19973071.2629265/150‑1 (MQ=60)

                                                                                GGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGC                                                                       >  SRR19973071.88674/1‑150 (MQ=60)

                                                                                 GTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCA                                                                      >  SRR19973071.845278/1‑150 (MQ=60)

                                                                                 GTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCA                                                                      >  SRR19973071.4151519/1‑150 (MQ=60)

                                                                                   TAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAAT                                                                    <  SRR19973071.2193211/150‑1 (MQ=60)

                                                                                     ACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAA                                                                  >  SRR19973071.885284/1‑150 (MQ=60)

                                                                                     ACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAA                                                                  >  SRR19973071.885289/1‑150 (MQ=60)

                                                                                      CGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAAT                                                                 <  SRR19973071.102014/150‑1 (MQ=60)

                                                                                       GGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATT                                                                >  SRR19973071.89046/1‑150 (MQ=60)

                                                                                       GGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATT                                                                >  SRR19973071.2490560/1‑150 (MQ=60)

                                                                                         AACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTG                                                              >  SRR19973071.3273371/1‑150 (MQ=60)

                                                                                         AACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTG                                                              <  SRR19973071.1598737/150‑1 (MQ=60)

                                                                                         AACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTG                                                              >  SRR19973071.4032233/1‑150 (MQ=60)

                                                                                            AATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCC                                                           <  SRR19973071.327882/150‑1 (MQ=60)

                                                                                            AATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCC                                                           >  SRR19973071.258395/1‑150 (MQ=60)

                                                                                              TCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCG                                                         >  SRR19973071.1138579/1‑150 (MQ=60)

                                                                                              TCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCG                                                         >  SRR19973071.1138882/1‑150 (MQ=60)

                                                                                                GCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGA                                                       <  SRR19973071.2217119/150‑1 (MQ=60)

                                                                                                GCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGA                                                       <  SRR19973071.2217121/150‑1 (MQ=60)

                                                                                                  TGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACG                                                     <  SRR19973071.1318821/150‑1 (MQ=60)

                                                                                                   GCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGC                                                    >  SRR19973071.4015427/1‑150 (MQ=60)

                                                                                                       GCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGA                                                >  SRR19973071.2263778/1‑150 (MQ=60)

                                                                                                        CAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGAC                                               >  SRR19973071.1873990/1‑150 (MQ=60)

                                                                                                        CAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGAC                                               >  SRR19973071.1381896/1‑150 (MQ=60)

                                                                                                         AAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACA                                              <  SRR19973071.1723119/150‑1 (MQ=60)

                                                                                                          AACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACAT                                             >  SRR19973071.1741885/1‑150 (MQ=60)

                                                                                                              AATCGATGTACCGACTATATCGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAG                                         <  SRR19973071.2220995/150‑1 (MQ=60)

                                                                                                              AATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAG                                         >  SRR19973071.4144009/1‑150 (MQ=60)

                                                                                                              AATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAG                                         >  SRR19973071.1250508/1‑150 (MQ=60)

                                                                                                                TCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCA                                       >  SRR19973071.4466518/1‑150 (MQ=60)

                                                                                                                  GATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGA                                     <  SRR19973071.1930987/150‑1 (MQ=60)

                                                                                                                  GATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGA                                     <  SRR19973071.51353/150‑1 (MQ=60)

                                                                                                                     GTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACG                                  >  SRR19973071.2168770/1‑150 (MQ=60)

                                                                                                                      TACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGC                                 >  SRR19973071.1819579/1‑150 (MQ=60)

                                                                                                                       ACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCC                                >  SRR19973071.2117210/1‑150 (MQ=60)

                                                                                                                        CCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCA                               <  SRR19973071.3871786/150‑1 (MQ=60)

                                                                                                                        CCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCA                               <  SRR19973071.688071/150‑1 (MQ=60)

                                                                                                                         CGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAA                              <  SRR19973071.4492968/150‑1 (MQ=60)

                                                                                                                            CAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCG                           >  SRR19973071.3552381/1‑150 (MQ=60)

                                                                                                                            CAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCG                           <  SRR19973071.3380871/150‑1 (MQ=60)

                                                                                                                            CAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCG                           >  SRR19973071.3552303/1‑150 (MQ=60)

                                                                                                                              ATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGC                         >  SRR19973071.4420088/1‑150 (MQ=60)

                                                                                                                               TAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCA                        <  SRR19973071.3551096/150‑1 (MQ=60)

                                                                                                                                       GTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATC                >  SRR19973071.1338995/1‑150 (MQ=60)

                                                                                                                                       GTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATC                <  SRR19973071.1228896/150‑1 (MQ=60)

                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               >  SRR19973071.174592/1‑150 (MQ=60)

                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               >  SRR19973071.2650331/1‑150 (MQ=60)

                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               >  SRR19973071.174443/1‑150 (MQ=60)

                                                                                                                                        TAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCA               >  SRR19973071.4642398/1‑150 (MQ=60)

                                                                                                                                          AACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACA             >  SRR19973071.1257693/1‑150 (MQ=60)

                                                                                                                                              GAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGC         >  SRR19973071.4437240/1‑150 (MQ=60)

                                                                                                                                               AATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCC        <  SRR19973071.1593684/150‑1 (MQ=60)

                                                                                                                                               AATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCC        <  SRR19973071.1593695/150‑1 (MQ=60)

                                                                                                                                                     TCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAA  <  SRR19973071.4390527/150‑1 (MQ=60)

CGGGAAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAA  >  CP009273/217738‑218036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`