Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 245,020 G→T pseudogene (1538/1713 nt) lfhA ← pseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273245,0200GT99.5% 671.9 / NA 202pseudogene (1538/1713 nt)lfhApseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (93/108);  total (94/108)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

CCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCTCCTG  >  CP009273/244874‑245165
                                                                                                                                                  |                                                                                                                                                 
ccAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCACAGGGCGCAGCTGCGGTGGCACCAGCCGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGGGGCa                                                                                                                                                >  2:3288165/1‑150 (MQ=255)
  aGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATc                                                                                                                                              >  1:2997522/1‑150 (MQ=255)
  aGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATc                                                                                                                                              >  1:145969/1‑150 (MQ=255)
  aGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATc                                                                                                                                              <  1:2976451/150‑1 (MQ=255)
         ccGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTActct                                                                                                                                       <  1:1245822/150‑1 (MQ=255)
           ggCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTgg                                                                                                                                     >  1:475914/1‑150 (MQ=255)
             cACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGaa                                                                                                                                   >  1:3708490/1‑150 (MQ=255)
               cTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACt                                                                                                                                 <  1:3419037/150‑1 (MQ=255)
               cTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACt                                                                                                                                 >  1:1764854/1‑150 (MQ=255)
                ttCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTg                                                                                                                                <  2:2506805/150‑1 (MQ=255)
                ttCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTg                                                                                                                                <  2:2092384/150‑1 (MQ=255)
                  cGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGt                                                                                                                              <  2:261806/150‑1 (MQ=255)
                    ttATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTg                                                                                                                            <  2:2782818/150‑1 (MQ=255)
                     tataCGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGa                                                                                                                           <  2:2368798/150‑1 (MQ=255)
                       taCGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGc                                                                                                                         <  1:1867521/150‑1 (MQ=255)
                         cGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCat                                                                                                                       <  1:1008643/150‑1 (MQ=255)
                         cGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCat                                                                                                                       <  1:3357527/150‑1 (MQ=255)
                           aCAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCatat                                                                                                                     >  2:795126/1‑150 (MQ=255)
                            cAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATAtt                                                                                                                    <  1:2355362/150‑1 (MQ=255)
                             aGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTc                                                                                                                   <  1:411359/150‑1 (MQ=255)
                                acaTGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGa                                                                                                                <  2:697180/150‑1 (MQ=255)
                                 caTGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGAt                                                                                                               <  2:3644304/150‑1 (MQ=255)
                                 caTGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGAt                                                                                                               >  1:489438/1‑150 (MQ=255)
                                      aGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGACCTGGTTGAGCATATTCGGATCTAcc                                                                                                          >  2:472406/1‑150 (MQ=255)
                                      aGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTAcc                                                                                                          <  1:2311759/150‑1 (MQ=255)
                                      aGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTAcc                                                                                                          <  2:2121106/150‑1 (MQ=255)
                                            ggCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACg                                                                                                    >  1:1517181/1‑150 (MQ=255)
                                              cgcAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCt                                                                                                  <  2:1834550/150‑1 (MQ=255)
                                               gcAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGATATTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTAGGATCTACCGGCACGCtt                                                                                                 >  1:331441/1‑149 (MQ=255)
                                                cAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGt                                                                                                >  1:584015/1‑150 (MQ=255)
                                                  aacaacCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCg                                                                                              <  1:2032399/150‑1 (MQ=255)
                                                   acaacCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGa                                                                                             >  1:1595259/1‑150 (MQ=255)
                                                    caacCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAg                                                                                            <  2:3464125/150‑1 (MQ=255)
                                                       ccTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCAt                                                                                         <  1:2891144/150‑1 (MQ=255)
                                                       ccTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCAt                                                                                         >  2:103910/1‑150 (MQ=255)
                                                           gCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACt                                                                                     <  2:2938371/150‑1 (MQ=255)
                                                              tAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTc                                                                                  <  1:1712831/150‑1 (MQ=255)
                                                               aaCGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTcc                                                                                 >  1:345270/1‑150 (MQ=255)
                                                               aaCGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTcc                                                                                 >  2:21269/1‑150 (MQ=255)
                                                                 cgcgCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCg                                                                               <  1:2179164/150‑1 (MQ=255)
                                                                   cgcCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGcc                                                                             >  1:2533924/1‑150 (MQ=255)
                                                                   cgcCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGcc                                                                             >  2:3600252/1‑150 (MQ=255)
                                                                     ccAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCt                                                                           <  2:884893/150‑1 (MQ=255)
                                                                     ccAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCt                                                                           <  2:3205364/150‑1 (MQ=255)
                                                                          aaaGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTgc                                                                      >  2:412362/1‑150 (MQ=255)
                                                                            aGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTgcgc                                                                    >  2:636904/1‑150 (MQ=255)
                                                                             gggCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGcc                                                                   >  2:875670/1‑150 (MQ=255)
                                                                             gggCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGcc                                                                   >  2:2845822/1‑150 (MQ=255)
                                                                                cgcAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGa                                                                >  1:1332243/1‑150 (MQ=255)
                                                                                 gcAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGAt                                                               <  2:258406/150‑1 (MQ=255)
                                                                                 gcAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGAt                                                               <  1:2542630/150‑1 (MQ=255)
                                                                                 gcAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGAt                                                               <  2:3539614/150‑1 (MQ=255)
                                                                                  cAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGAtt                                                              <  2:2298451/150‑1 (MQ=255)
                                                                                   aGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGGTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTc                                                             <  2:2420720/150‑1 (MQ=255)
                                                                                     cTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTcac                                                           >  2:2816020/1‑150 (MQ=255)
                                                                                     cTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTcac                                                           <  2:1312656/150‑1 (MQ=255)
                                                                                     cTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTcac                                                           >  1:829264/1‑150 (MQ=255)
                                                                                      tGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTcacc                                                          <  2:173784/150‑1 (MQ=255)
                                                                                       gCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTcacca                                                         <  2:1076115/150‑1 (MQ=255)
                                                                                        cGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGTGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTcaccac                                                        <  1:1105478/150‑1 (MQ=255)
                                                                                        cGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTcaccac                                                        <  2:194548/150‑1 (MQ=255)
                                                                                         ggTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACg                                                       >  2:1864633/1‑150 (MQ=255)
                                                                                           tGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGtt                                                     >  1:1885517/1‑150 (MQ=255)
                                                                                            ggCACCAGCGGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTg                                                    <  2:2801110/150‑1 (MQ=255)
                                                                                             gCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTgg                                                   <  2:3163505/150‑1 (MQ=255)
                                                                                             gCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTgg                                                   <  2:2997522/150‑1 (MQ=255)
                                                                                             gCACCAGCAGCACCGGGTCTGTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTgg                                                   <  2:3120115/150‑1 (MQ=255)
                                                                                              cACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGt                                                  <  2:3244405/150‑1 (MQ=255)
                                                                                              cACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGt                                                  <  1:548561/150‑1 (MQ=255)
                                                                                              cACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGt                                                  <  2:2177232/150‑1 (MQ=255)
                                                                                               aCCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTc                                                 >  2:2052014/1‑150 (MQ=255)
                                                                                                 cagcagCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCAGTTGCGCCTGATTCACCACGTTGGTcag                                               >  1:1303127/1‑150 (MQ=255)
                                                                                                   gcagcaCCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTcagca                                             <  1:3409888/150‑1 (MQ=255)
                                                                                                    cagcaCCGGGTCTGTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTcagcag                                            <  2:2893264/150‑1 (MQ=255)
                                                                                                      gcaCCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGAt                                          >  1:344331/1‑150 (MQ=255)
                                                                                                       caCCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGAtt                                         >  1:2244504/1‑150 (MQ=255)
                                                                                                         ccGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCTCTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGAtttt                                       <  2:1509502/150‑1 (MQ=255)
                                                                                                         ccGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGAtttt                                       <  2:1511826/150‑1 (MQ=255)
                                                                                                           gggTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTcc                                     >  1:577593/1‑150 (MQ=255)
                                                                                                           gggTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTcc                                     >  2:3769727/1‑150 (MQ=255)
                                                                                                            ggTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCa                                    >  2:284096/1‑150 (MQ=255)
                                                                                                             gTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAg                                   <  1:974965/150‑1 (MQ=255)
                                                                                                               cTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCt                                 <  1:1579564/150‑1 (MQ=255)
                                                                                                                tttCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTc                                <  2:1231717/150‑1 (MQ=255)
                                                                                                                     ccgccgCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCattat                           >  1:1171760/1‑150 (MQ=255)
                                                                                                                      cgccgcTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCattatt                          <  2:2290649/150‑1 (MQ=255)
                                                                                                                       gccgcTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTc                         >  2:3269276/1‑150 (MQ=255)
                                                                                                                             ttCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGta                   >  2:3406060/1‑150 (MQ=255)
                                                                                                                              tCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGtat                  <  2:1049038/150‑1 (MQ=255)
                                                                                                                                aTCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATac                >  1:3615842/1‑150 (MQ=255)
                                                                                                                                aTCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATac                <  1:3665546/150‑1 (MQ=255)
                                                                                                                                  cTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATacac              >  1:2206730/1‑150 (MQ=255)
                                                                                                                                  cTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATacac              <  2:303580/150‑1 (MQ=255)
                                                                                                                                   tGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACAcc             >  1:2810642/1‑150 (MQ=255)
                                                                                                                                      tctTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTc          <  2:829264/150‑1 (MQ=255)
                                                                                                                                      tctTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTGTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTc          <  1:2471833/150‑1 (MQ=255)
                                                                                                                                        tttCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAg        >  2:3721302/1‑150 (MQ=255)
                                                                                                                                         ttCATCTGTTGCATCGTACTCTGGAAATGGTTGAGCATATTAGGATCTACCGGCTCGATGTCGAGTATCACTTTCCCGACCTGTTGCGCATGATTCACCACTTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGc       <  1:3276356/150‑1 (MQ=255)
                                                                                                                                           cACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCTc     >  2:3582927/1‑150 (MQ=255)
                                                                                                                                            aCCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCTcc    <  1:1463743/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCTCCTG  >  CP009273/244874‑245165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCTCC  >  CP009273/244876‑245163
                                                                                                                                                |                                                                                                                                               
AGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATC                                                                                                                                            >  SRR19973061.146438/1‑150 (MQ=60)
AGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATC                                                                                                                                            <  SRR19973061.2984130/150‑1 (MQ=60)
AGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATC                                                                                                                                            >  SRR19973061.3005256/1‑150 (MQ=60)
AGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTGCACCTGTTGCATC                                                                                                                                            <  SRR19973061.2982304/150‑1 (MQ=60)
  CTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGT                                                                                                                                          >  SRR19973061.360045/1‑150 (MQ=60)
   TCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTA                                                                                                                                         >  SRR19973061.1308623/1‑150 (MQ=60)
   TCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTA                                                                                                                                         >  SRR19973061.42055/1‑150 (MQ=60)
    CATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTAC                                                                                                                                        <  SRR19973061.1181060/150‑1 (MQ=60)
     ATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACT                                                                                                                                       >  SRR19973061.241096/1‑150 (MQ=60)
       CCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCT                                                                                                                                     <  SRR19973061.1248907/150‑1 (MQ=60)
        CGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTG                                                                                                                                    >  SRR19973061.1770475/1‑150 (MQ=60)
        CGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTG                                                                                                                                    <  SRR19973061.2501107/150‑1 (MQ=60)
         GGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGG                                                                                                                                   >  SRR19973061.477195/1‑150 (MQ=60)
           CACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAA                                                                                                                                 <  SRR19973061.3358026/150‑1 (MQ=60)
           CACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAA                                                                                                                                 >  SRR19973061.3717971/1‑150 (MQ=60)
             CTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACT                                                                                                                               <  SRR19973061.3427813/150‑1 (MQ=60)
             CTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACT                                                                                                                               >  SRR19973061.1769276/1‑150 (MQ=60)
             CTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACT                                                                                                                               >  SRR19973061.174318/1‑150 (MQ=60)
             CTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACT                                                                                                                               <  SRR19973061.885494/150‑1 (MQ=60)
              TTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTG                                                                                                                              <  SRR19973061.582821/150‑1 (MQ=60)
                  TTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTG                                                                                                                          <  SRR19973061.500650/150‑1 (MQ=60)
                   TATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGA                                                                                                                         <  SRR19973061.368144/150‑1 (MQ=60)
                     TACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGC                                                                                                                       <  SRR19973061.1872176/150‑1 (MQ=60)
                       CGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCAT                                                                                                                     <  SRR19973061.1011219/150‑1 (MQ=60)
                       CGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCAT                                                                                                                     <  SRR19973061.3366164/150‑1 (MQ=60)
                          CAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATT                                                                                                                  <  SRR19973061.2361348/150‑1 (MQ=60)
                           AGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTC                                                                                                                 >  SRR19973061.2640331/1‑150 (MQ=60)
                           AGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTC                                                                                                                 <  SRR19973061.412482/150‑1 (MQ=60)
                           AGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTGTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTC                                                                                                                 <  SRR19973061.2323887/150‑1 (MQ=60)
                             CACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGG                                                                                                               <  SRR19973061.1987809/150‑1 (MQ=60)
                               CATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGAT                                                                                                             >  SRR19973061.490724/1‑150 (MQ=60)
                               CATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGAT                                                                                                             >  SRR19973061.490742/1‑150 (MQ=60)
                                  GCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTA                                                                                                          <  SRR19973061.3294074/150‑1 (MQ=60)
                                    AGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACC                                                                                                        <  SRR19973061.2317621/150‑1 (MQ=60)
                                    AGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACC                                                                                                        <  SRR19973061.3396036/150‑1 (MQ=60)
                                    AGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACC                                                                                                        <  SRR19973061.2304569/150‑1 (MQ=60)
                                       CCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGC                                                                                                     >  SRR19973061.1018852/1‑150 (MQ=60)
                                          GGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACG                                                                                                  >  SRR19973061.1520976/1‑150 (MQ=60)
                                             GCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTG                                                                                               <  SRR19973061.1379201/150‑1 (MQ=60)
                                             GCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGATATTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTAGGATCTACCGGCACGCTT                                                                                               >  SRR19973061.332314/1‑150 (MQ=60)
                                              CAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGT                                                                                              >  SRR19973061.585537/1‑150 (MQ=60)
                                               AAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTC                                                                                             >  SRR19973061.342223/1‑150 (MQ=60)
                                                AACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCG                                                                                            <  SRR19973061.2037502/150‑1 (MQ=60)
                                                 ACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGA                                                                                           >  SRR19973061.1599220/1‑150 (MQ=60)
                                                     CCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCAT                                                                                       <  SRR19973061.2898559/150‑1 (MQ=60)
                                                        TGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCAC                                                                                    >  SRR19973061.1465559/1‑150 (MQ=60)
                                                           ATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCCTCACTTT                                                                                 >  SRR19973061.3443414/1‑150 (MQ=60)
                                                            TAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTC                                                                                <  SRR19973061.1717132/150‑1 (MQ=60)
                                                             AACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCC                                                                               >  SRR19973061.346189/1‑150 (MQ=60)
                                                             AACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCC                                                                               >  SRR19973061.1712001/1‑150 (MQ=60)
                                                               CGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCG                                                                             <  SRR19973061.2184641/150‑1 (MQ=60)
                                                               CGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCG                                                                             <  SRR19973061.2184829/150‑1 (MQ=60)
                                                                 CGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCC                                                                           >  SRR19973061.2540377/1‑150 (MQ=60)
                                                                         AAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACAGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCG                                                                   >  SRR19973061.1284571/1‑150 (MQ=60)
                                                                              CGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGA                                                              >  SRR19973061.1335590/1‑150 (MQ=60)
                                                                               GCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGAT                                                             <  SRR19973061.2549099/150‑1 (MQ=60)
                                                                                   CTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCAC                                                         >  SRR19973061.831427/1‑150 (MQ=60)
                                                                                   CTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCAC                                                         <  SRR19973061.3741793/150‑1 (MQ=60)
                                                                                    TGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACC                                                        >  SRR19973061.254883/1‑150 (MQ=60)
                                                                                      CGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGTGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCAC                                                      <  SRR19973061.1108305/150‑1 (MQ=60)
                                                                                      CGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCAC                                                      >  SRR19973061.2220761/1‑150 (MQ=60)
                                                                                         TGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTT                                                   >  SRR19973061.1889486/1‑150 (MQ=60)
                                                                                         TGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTT                                                   >  SRR19973061.1890203/1‑150 (MQ=60)
                                                                                            CACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGT                                                <  SRR19973061.550008/150‑1 (MQ=60)
                                                                                               CAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCAGTTGCGCCTGATTCACCACGTTGGTCAG                                             >  SRR19973061.1306374/1‑150 (MQ=60)
                                                                                                 GCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCA                                           <  SRR19973061.3418644/150‑1 (MQ=60)
                                                                                                    GCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGAT                                        >  SRR19973061.345248/1‑150 (MQ=60)
                                                                                                    GCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACATTCCCGCCCAGTTGCGCCTGATTCACCCCGTTGGTCAGCAGAT                                        >  SRR19973061.345375/1‑150 (MQ=60)
                                                                                                     CACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATT                                       <  SRR19973061.724438/150‑1 (MQ=60)
                                                                                                     CACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATT                                       >  SRR19973061.2250145/1‑150 (MQ=60)
                                                                                                         GGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCAGATTCACCACGTTGGTCAGCAGATTTTCC                                   >  SRR19973061.579099/1‑150 (MQ=60)
                                                                                                         GGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCAGTCGAGCAACACTATCCCGCCCTGTTGCGCCGGATTCACCACGTTGGTCAGCAGATTTTCC                                   >  SRR19973061.56050/1‑150 (MQ=60)
                                                                                                           GTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAG                                 <  SRR19973061.977447/150‑1 (MQ=60)
                                                                                                             CTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCT                               <  SRR19973061.1583485/150‑1 (MQ=60)
                                                                                                             CTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCT                               <  SRR19973061.288257/150‑1 (MQ=60)
                                                                                                                TCCCCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCAT                            >  SRR19973061.1560971/1‑150 (MQ=60)
                                                                                                                   CCGCCGCTTTCATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTAT                         >  SRR19973061.1174724/1‑150 (MQ=60)
                                                                                                                              ATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATAC              <  SRR19973061.3674916/150‑1 (MQ=60)
                                                                                                                              ATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATAC              >  SRR19973061.3625176/1‑150 (MQ=60)
                                                                                                                              ATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATAC              >  SRR19973061.2987254/1‑150 (MQ=60)
                                                                                                                              ATCTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATAC              >  SRR19973061.3625091/1‑150 (MQ=60)
                                                                                                                                CTGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACAC            >  SRR19973061.2212276/1‑150 (MQ=60)
                                                                                                                                 TGCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACC           >  SRR19973061.2817855/1‑150 (MQ=60)
                                                                                                                                  GCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCG          >  SRR19973061.1591930/1‑150 (MQ=60)
                                                                                                                                  GCTCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCG          >  SRR19973061.1504084/1‑150 (MQ=60)
                                                                                                                                    TCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTC        >  SRR19973061.2098043/1‑150 (MQ=60)
                                                                                                                                    TCTTTCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTGTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTC        <  SRR19973061.2478128/150‑1 (MQ=60)
                                                                                                                                       TTCATCTGTTGCATCGTACTCTGGAAATGGTTGAGCATATTAGGATCTACCGGCTCGATGTCGAGTATCACTTTCCCGACCTGTTGCGCATGATTCACCACTTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGC     <  SRR19973061.3284793/150‑1 (MQ=60)
                                                                                                                                        TCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCT    >  SRR19973061.1132833/1‑150 (MQ=60)
                                                                                                                                        TCACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCT    >  SRR19973061.3038346/1‑150 (MQ=60)
                                                                                                                                          ACCTGTTGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCTACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCTCC  <  SRR19973061.1467426/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                               
AGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACTTTCCCGCCCTGTTGCGCCTGATTCACCACGTTGGTCAGCAGATTTTCCAGCTCATTATTCAGCGTATACACCGTCAGCTCC  >  CP009273/244876‑245163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑