Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 459,325 T→A intergenic (+83/‑68) ppiD → / → ybaV periplasmic folding chaperone, has an inactive PPIase domain/putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273459,3250TA100.0% 709.3 / NA 215intergenic (+83/‑68)ppiD/ybaVperiplasmic folding chaperone, has an inactive PPIase domain/putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (101/114);  total (101/114)

Only 100 of 215 total aligned reads displayed.

TCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAG  >  CP009273/459179‑459471
                                                                                                                                                  |                                                                                                                                                  
tctGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGcc                                                                                                                                                 <  1:2671211/150‑1 (MQ=255)
tctGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGcc                                                                                                                                                 <  1:737982/150‑1 (MQ=255)
  tgatgaGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAt                                                                                                                                               >  1:636524/1‑150 (MQ=255)
    atgaGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTTGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAttt                                                                                                                                             <  1:813353/150‑1 (MQ=255)
      gaGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGt                                                                                                                                           <  1:958945/150‑1 (MQ=255)
         tAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTa                                                                                                                                        <  1:3524920/150‑1 (MQ=255)
           aCCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACt                                                                                                                                      >  2:2949180/1‑150 (MQ=255)
            ccTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTg                                                                                                                                     <  2:2029310/150‑1 (MQ=255)
              tGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtt                                                                                                                                   >  1:3739345/1‑150 (MQ=255)
              tGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtt                                                                                                                                   >  2:1246651/1‑150 (MQ=255)
                cGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtttt                                                                                                                                 <  1:3513979/150‑1 (MQ=255)
                cGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtttt                                                                                                                                 >  1:978807/1‑150 (MQ=255)
                cGTAAAGAGGCGAAAATCAAAATTGGCGACGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtttt                                                                                                                                 <  2:1146791/150‑1 (MQ=255)
                 gTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTc                                                                                                                                >  2:3740013/1‑150 (MQ=255)
                   aaaGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCAc                                                                                                                              <  2:1505118/150‑1 (MQ=255)
                   aaaGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCAc                                                                                                                              >  2:2702593/1‑150 (MQ=255)
                    aaGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACt                                                                                                                             <  2:1497119/150‑1 (MQ=255)
                    aaGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACt                                                                                                                             <  2:2217377/150‑1 (MQ=255)
                       agGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGcc                                                                                                                          <  1:2398440/150‑1 (MQ=255)
                       agGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGcc                                                                                                                          >  2:321893/1‑150 (MQ=255)
                             aaaTCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAg                                                                                                                    <  1:3580502/150‑1 (MQ=255)
                             aaaTCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTACTGAACATAGCCATATGTTTACTGTTTTCACTGCCGTTAAg                                                                                                                    >  1:126767/1‑150 (MQ=255)
                              aaTCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAgg                                                                                                                   >  1:1831936/1‑150 (MQ=255)
                                  aaaaTTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGAt                                                                                                               >  1:2780843/1‑150 (MQ=255)
                                        ggCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACt                                                                                                         <  1:1137359/150‑1 (MQ=255)
                                           gATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGtt                                                                                                      <  2:876014/150‑1 (MQ=255)
                                           gATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGtt                                                                                                      <  1:3769617/150‑1 (MQ=255)
                                           gATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGtt                                                                                                      >  2:3077070/1‑150 (MQ=255)
                                               cgcTGGAACAGCAATACTCCTGACGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGCACCGAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaaca                                                                                                  <  1:1019974/150‑1 (MQ=255)
                                                 cTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaacaaa                                                                                                >  2:2187760/1‑150 (MQ=255)
                                                  tGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaacaaac                                                                                               <  1:1072519/150‑1 (MQ=255)
                                                  tGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaacaaac                                                                                               <  1:3268217/150‑1 (MQ=255)
                                                   ggAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaacaaaca                                                                                              <  2:3402414/150‑1 (MQ=255)
                                                    gAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAacaaacaa                                                                                             >  2:1070356/1‑150 (MQ=255)
                                                     aaCAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAg                                                                                            <  2:62731/150‑1 (MQ=255)
                                                     aaCAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAg                                                                                            <  1:2071378/150‑1 (MQ=255)
                                                       cAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGga                                                                                          <  2:2125896/150‑1 (MQ=255)
                                                          cAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGaa                                                                                       >  2:625498/1‑150 (MQ=255)
                                                            ataatCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGaaaa                                                                                     >  2:3235689/1‑150 (MQ=255)
                                                              aatCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACa                                                                                   <  2:682489/150‑1 (MQ=255)
                                                              aatCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACa                                                                                   <  2:1331342/150‑1 (MQ=255)
                                                              aatCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACa                                                                                   >  1:3796637/1‑150 (MQ=255)
                                                                  cTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTAt                                                                               <  2:102737/150‑1 (MQ=255)
                                                                   tGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTTTAACAAGAAAATGTCTCTTTCGCGGTCTTTTCAATTTCTGAACATAGCCATTTTTTTACTGTTTTCACTGCAGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATg                                                                              <  1:175578/150‑1 (MQ=255)
                                                                   tGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATg                                                                              <  1:1947133/150‑1 (MQ=255)
                                                                      aGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGaaa                                                                           <  1:2221897/150‑1 (MQ=255)
                                                                      aGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGaaa                                                                           <  1:311847/150‑1 (MQ=255)
                                                                       gccgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAac                                                                          <  1:1506388/150‑1 (MQ=255)
                                                                       gccgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAac                                                                          <  1:3415541/150‑1 (MQ=255)
                                                                        ccgccTCGCATAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAaca                                                                         <  2:2258371/150‑1 (MQ=255)
                                                                        ccgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAaca                                                                         >  2:2226990/1‑150 (MQ=255)
                                                                        ccgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAaca                                                                         >  2:3224349/1‑150 (MQ=255)
                                                                         cgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAacac                                                                        >  2:1627753/1‑150 (MQ=255)
                                                                           ccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACgg                                                                      >  1:136490/1‑150 (MQ=255)
                                                                                cAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTa                                                                 <  2:3777387/150‑1 (MQ=255)
                                                                                cAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTa                                                                 <  1:2575394/150‑1 (MQ=255)
                                                                                 aaaaaaaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTaa                                                                <  2:636565/150‑1 (MQ=255)
                                                                                  aaaaaaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTaaa                                                               <  1:3254801/150‑1 (MQ=255)
                                                                                    aaaaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGc                                                             <  2:315235/150‑1 (MQ=255)
                                                                                      aaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCAc                                                           >  2:3658985/1‑150 (MQ=255)
                                                                                      aaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCAc                                                           >  2:3659092/1‑150 (MQ=255)
                                                                                        tGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTg                                                         <  1:1681775/150‑1 (MQ=255)
                                                                                          cGTTGCAGCTGTAACAAGAATAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACCTAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCt                                                       <  2:2377201/150‑1 (MQ=255)
                                                                                           gTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTc                                                      <  1:2935649/150‑1 (MQ=255)
                                                                                            ttGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCa                                                     >  1:2677090/1‑150 (MQ=255)
                                                                                            ttGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTCCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCa                                                     >  1:420402/1‑150 (MQ=255)
                                                                                             tGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCAt                                                    <  1:2070662/150‑1 (MQ=255)
                                                                                               cAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTa                                                  >  2:1804072/1‑150 (MQ=255)
                                                                                                  cTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTAccc                                               <  1:34321/150‑1 (MQ=255)
                                                                                                    gTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTg                                             >  2:1743988/1‑150 (MQ=255)
                                                                                                    gTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTg                                             <  1:2356633/150‑1 (MQ=255)
                                                                                                     tAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGt                                            <  1:220438/150‑1 (MQ=255)
                                                                                                       aCAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTcc                                          >  2:2248246/1‑150 (MQ=255)
                                                                                                        cAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTccc                                         <  2:2742061/150‑1 (MQ=255)
                                                                                                         aaGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCt                                        >  2:750665/1‑150 (MQ=255)
                                                                                                         aaGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCt                                        >  2:2820757/1‑150 (MQ=255)
                                                                                                         aaGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCt                                        <  1:1286463/150‑1 (MQ=255)
                                                                                                          aGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTg                                       <  2:2033418/150‑1 (MQ=255)
                                                                                                           gAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTgg                                      <  1:3172653/150‑1 (MQ=255)
                                                                                                           gAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTgg                                      >  1:1801791/1‑150 (MQ=255)
                                                                                                            aaaaGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGc                                     <  1:388429/150‑1 (MQ=255)
                                                                                                              aaGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCt                                   >  1:3621431/1‑150 (MQ=255)
                                                                                                              aaGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCt                                   <  2:2859917/150‑1 (MQ=255)
                                                                                                              aaGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAATATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCt                                   >  1:2747057/1‑150 (MQ=255)
                                                                                                                   cGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGcc                              >  2:3435315/1‑150 (MQ=255)
                                                                                                                   cGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGcc                              <  1:1919601/150‑1 (MQ=255)
                                                                                                                    gCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCg                             >  1:3170747/1‑150 (MQ=255)
                                                                                                                     cTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCgg                            >  1:1879628/1‑150 (MQ=255)
                                                                                                                      tttCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGa                           >  1:1218649/1‑150 (MQ=255)
                                                                                                                         cgcgGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATg                        >  1:487170/1‑150 (MQ=255)
                                                                                                                          gcgGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTCTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGt                       <  2:1275126/150‑1 (MQ=255)
                                                                                                                           cgGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGtc                      >  2:2404043/1‑150 (MQ=255)
                                                                                                                               cTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCAt                  <  2:3708613/150‑1 (MQ=255)
                                                                                                                               cTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCAt                  >  2:461898/1‑150 (MQ=255)
                                                                                                                                    ccATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAgcgc             >  2:2432954/1‑150 (MQ=255)
                                                                                                                                          cTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTggc       <  2:3738662/150‑1 (MQ=255)
                                                                                                                                           tGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTggcg      <  1:321893/150‑1 (MQ=255)
                                                                                                                                             aaCATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGAGCCGGAATGTCTCATAGCGCGCTggcggc    >  2:1982516/1‑150 (MQ=255)
                                                                                                                                              aCATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCa   <  1:1246651/150‑1 (MQ=255)
                                                                                                                                              aCATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCa   >  2:2954405/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAG  >  CP009273/459179‑459471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTT  >  CP009273/459176‑459474
                                                                                                                                                     |                                                                                                                                                     
AGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATA                                                                                                                                                       <  SRR19973061.2374184/150‑1 (MQ=60)
 GCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAG                                                                                                                                                      <  SRR19973061.2211355/150‑1 (MQ=60)
   TCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCC                                                                                                                                                    <  SRR19973061.2678085/150‑1 (MQ=60)
   TCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCC                                                                                                                                                    <  SRR19973061.739916/150‑1 (MQ=60)
    CTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCA                                                                                                                                                   >  SRR19973061.3522170/1‑150 (MQ=60)
     TGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAT                                                                                                                                                  >  SRR19973061.3434108/1‑150 (MQ=60)
     TGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAT                                                                                                                                                  >  SRR19973061.409301/1‑150 (MQ=60)
     TGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAT                                                                                                                                                  >  SRR19973061.638141/1‑150 (MQ=60)
     TGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAT                                                                                                                                                  >  SRR19973061.638182/1‑150 (MQ=60)
     TGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAT                                                                                                                                                  <  SRR19973061.735202/150‑1 (MQ=60)
       ATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTTGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTT                                                                                                                                                <  SRR19973061.815480/150‑1 (MQ=60)
       ATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTT                                                                                                                                                >  SRR19973061.992805/1‑150 (MQ=60)
         GAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGT                                                                                                                                              <  SRR19973061.1259053/150‑1 (MQ=60)
         GAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGT                                                                                                                                              <  SRR19973061.961362/150‑1 (MQ=60)
         GAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGT                                                                                                                                              <  SRR19973061.961436/150‑1 (MQ=60)
           GTAACCTGCGTAAAGAGGAGAAAATAAAAATTGGCGATGCGTTGGAACAGAAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGTCCTTTTCCATTTCTGAACATAGCCATTTGTTT                                                                                                                                            <  SRR19973061.2635689/150‑1 (MQ=60)
            TAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTA                                                                                                                                           <  SRR19973061.3533949/150‑1 (MQ=60)
            TAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTA                                                                                                                                           <  SRR19973061.3533916/150‑1 (MQ=60)
               CCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGACCCTAGCCATTTGTTTACTG                                                                                                                                        >  SRR19973061.2031278/1‑150 (MQ=60)
                 TGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTT                                                                                                                                      >  SRR19973061.3748917/1‑150 (MQ=60)
                 TGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTT                                                                                                                                      >  SRR19973061.3748231/1‑150 (MQ=60)
                 TGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTT                                                                                                                                      <  SRR19973061.1947086/150‑1 (MQ=60)
                   CGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTT                                                                                                                                    >  SRR19973061.981296/1‑150 (MQ=60)
                   CGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTT                                                                                                                                    <  SRR19973061.3522975/150‑1 (MQ=60)
                    GTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTC                                                                                                                                   <  SRR19973061.38577/150‑1 (MQ=60)
                      AAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCAC                                                                                                                                 <  SRR19973061.2520191/150‑1 (MQ=60)
                       AAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACT                                                                                                                                <  SRR19973061.1216531/150‑1 (MQ=60)
                          AGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCC                                                                                                                             <  SRR19973061.2404555/150‑1 (MQ=60)
                             CGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTT                                                                                                                          >  SRR19973061.1828251/1‑150 (MQ=60)
                             CGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTT                                                                                                                          >  SRR19973061.3695889/1‑150 (MQ=60)
                              GAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTA                                                                                                                         >  SRR19973061.3408191/1‑150 (MQ=60)
                              GAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTA                                                                                                                         <  SRR19973061.2224328/150‑1 (MQ=60)
                               AAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAA                                                                                                                        >  SRR19973061.2867240/1‑150 (MQ=60)
                                AAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGTAATTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAG                                                                                                                       <  SRR19973061.3590832/150‑1 (MQ=60)
                                AAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAG                                                                                                                       <  SRR19973061.3589681/150‑1 (MQ=60)
                                AAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTACTGAACATAGCCATATGTTTACTGTTTTCACTGCCGTTAAG                                                                                                                       >  SRR19973061.127173/1‑150 (MQ=60)
                                 AATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGG                                                                                                                      >  SRR19973061.1836530/1‑150 (MQ=60)
                                  ATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGT                                                                                                                     <  SRR19973061.1874810/150‑1 (MQ=60)
                                    CAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGA                                                                                                                   >  SRR19973061.2840327/1‑150 (MQ=60)
                                     AAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGAT                                                                                                                  >  SRR19973061.2787997/1‑150 (MQ=60)
                                           GGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACT                                                                                                            <  SRR19973061.1140255/150‑1 (MQ=60)
                                             CGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGT                                                                                                          >  SRR19973061.2764449/1‑150 (MQ=60)
                                              GATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTT                                                                                                         <  SRR19973061.3779244/150‑1 (MQ=60)
                                                TGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAA                                                                                                       >  SRR19973061.1133903/1‑150 (MQ=60)
                                                  CGCTGGAACAGCAATACTCCTGACGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGCACCGAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACA                                                                                                     <  SRR19973061.1022572/150‑1 (MQ=60)
                                                     TGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAAC                                                                                                  >  SRR19973061.2975026/1‑150 (MQ=60)
                                                     TGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAAC                                                                                                  <  SRR19973061.3276636/150‑1 (MQ=60)
                                                     TGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAAC                                                                                                  <  SRR19973061.3276628/150‑1 (MQ=60)
                                                     TGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAAC                                                                                                  <  SRR19973061.1075269/150‑1 (MQ=60)
                                                        AACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAG                                                                                               <  SRR19973061.2076582/150‑1 (MQ=60)
                                                        AACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAG                                                                                               <  SRR19973061.2076537/150‑1 (MQ=60)
                                                           AGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAAAAAACAAGGAG                                                                                            >  SRR19973061.451579/1‑150 (MQ=60)
                                                                 AATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACA                                                                                      >  SRR19973061.3806317/1‑150 (MQ=60)
                                                                 AATCCCGAAGCAGCCTAGCAAAAAAATGCGTTGCAGCTGTAACAAGAATAGGTCGCTTTCGCGTCCTTTTCAATTTCTGAACATAGACATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACA                                                                                      <  SRR19973061.3240418/150‑1 (MQ=60)
                                                                     CTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTAT                                                                                  <  SRR19973061.861827/150‑1 (MQ=60)
                                                                      TGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTTTAACAAGAAAATGTCTCTTTCGCGGTCTTTTCAATTTCTGAACATAGCCATTTTTTTACTGTTTTCACTGCAGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATG                                                                                 <  SRR19973061.176114/150‑1 (MQ=60)
                                                                      TGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATG                                                                                 <  SRR19973061.1951986/150‑1 (MQ=60)
                                                                         AGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAA                                                                              <  SRR19973061.2227471/150‑1 (MQ=60)
                                                                         AGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAA                                                                              <  SRR19973061.312665/150‑1 (MQ=60)
                                                                          GCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAAC                                                                             <  SRR19973061.1510160/150‑1 (MQ=60)
                                                                          GCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAAC                                                                             <  SRR19973061.3424308/150‑1 (MQ=60)
                                                                           CCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAATAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACA                                                                            <  SRR19973061.2631664/150‑1 (MQ=60)
                                                                            CGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACAC                                                                           <  SRR19973061.1299883/150‑1 (MQ=60)
                                                                            CGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACAC                                                                           >  SRR19973061.3538396/1‑150 (MQ=60)
                                                                              CCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGG                                                                         >  SRR19973061.136926/1‑150 (MQ=60)
                                                                                  GCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATT                                                                     >  SRR19973061.2369734/1‑150 (MQ=60)
                                                                                   CAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTA                                                                    >  SRR19973061.1711132/1‑150 (MQ=60)
                                                                                   CAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTA                                                                    >  SRR19973061.2749115/1‑150 (MQ=60)
                                                                                   CAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTA                                                                    <  SRR19973061.2581987/150‑1 (MQ=60)
                                                                                     AAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAA                                                                  <  SRR19973061.3263174/150‑1 (MQ=60)
                                                                                         AATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCAC                                                              <  SRR19973061.1789140/150‑1 (MQ=60)
                                                                                           TGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTG                                                            <  SRR19973061.1686000/150‑1 (MQ=60)
                                                                                           TGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTG                                                            <  SRR19973061.3749587/150‑1 (MQ=60)
                                                                                             CGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCT                                                          >  SRR19973061.3783890/1‑150 (MQ=60)
                                                                                             CGTTGCAGCTGTAACAAGAAAAGGTCGCTTTAGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCT                                                          <  SRR19973061.3156796/150‑1 (MQ=60)
                                                                                              GTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTC                                                         <  SRR19973061.2122226/150‑1 (MQ=60)
                                                                                              GTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTC                                                         <  SRR19973061.2943201/150‑1 (MQ=60)
                                                                                               TTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCA                                                        >  SRR19973061.774193/1‑150 (MQ=60)
                                                                                               TTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCA                                                        >  SRR19973061.2683978/1‑150 (MQ=60)
                                                                                               TTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTCCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCA                                                        >  SRR19973061.421541/1‑150 (MQ=60)
                                                                                                TGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCAT                                                       <  SRR19973061.159594/150‑1 (MQ=60)
                                                                                                TGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCAT                                                       <  SRR19973061.2075863/150‑1 (MQ=60)
                                                                                                  CAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTA                                                     >  SRR19973061.2683581/1‑150 (MQ=60)
                                                                                                   AGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTAC                                                    >  SRR19973061.3180610/1‑150 (MQ=60)
                                                                                                    GCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACC                                                   <  SRR19973061.3196494/150‑1 (MQ=60)
                                                                                                     CTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCC                                                  <  SRR19973061.34444/150‑1 (MQ=60)
                                                                                                      TGTAACAAGAAAAGGTCGCTTTCGCGGTCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCT                                                 <  SRR19973061.585919/150‑1 (MQ=60)
                                                                                                       GTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTG                                                <  SRR19973061.1444805/150‑1 (MQ=60)
                                                                                                       GTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTG                                                <  SRR19973061.2362620/150‑1 (MQ=60)
                                                                                                        TAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGT                                               <  SRR19973061.221086/150‑1 (MQ=60)
                                                                                                         AACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTC                                              >  SRR19973061.2306130/1‑150 (MQ=60)
                                                                                                            AAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCT                                           <  SRR19973061.1289652/150‑1 (MQ=60)
                                                                                                              GAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGG                                         >  SRR19973061.1806291/1‑150 (MQ=60)
                                                                                                              GAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGG                                         <  SRR19973061.3180794/150‑1 (MQ=60)
                                                                                                               AAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGC                                        <  SRR19973061.389490/150‑1 (MQ=60)
                                                                                                                 AAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCT                                      >  SRR19973061.3630697/1‑150 (MQ=60)
                                                                                                                 AAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAATATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCT                                      >  SRR19973061.2754128/1‑150 (MQ=60)
                                                                                                                      CGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCC                                 <  SRR19973061.1924361/150‑1 (MQ=60)
                                                                                                                       GCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCG                                >  SRR19973061.3178882/1‑150 (MQ=60)
                                                                                                                        CTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGG                               >  SRR19973061.1884301/1‑150 (MQ=60)
                                                                                                                        CTTTCGCGGCCTTTTCCAATTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGG                               >  SRR19973061.1884789/1‑150 (MQ=60)
                                                                                                                         TTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGA                              >  SRR19973061.1221695/1‑150 (MQ=60)
                                                                                                                         TTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGA                              >  SRR19973061.1222193/1‑150 (MQ=60)
                                                                                                                            CGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATG                           >  SRR19973061.488472/1‑150 (MQ=60)
                                                                                                                                GCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTC                       >  SRR19973061.1035370/1‑150 (MQ=60)
                                                                                                                                           TTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTG            <  SRR19973061.3307701/150‑1 (MQ=60)
                                                                                                                                             CTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGC          >  SRR19973061.1304708/1‑150 (MQ=60)
                                                                                                                                              TGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCG         <  SRR19973061.322732/150‑1 (MQ=60)
                                                                                                                                               GAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGG        >  SRR19973061.2231091/1‑150 (MQ=60)
                                                                                                                                                 ACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCA      <  SRR19973061.1249740/150‑1 (MQ=60)
                                                                                                                                                  CATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAG     <  SRR19973061.1903648/150‑1 (MQ=60)
                                                                                                                                                   ATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGC    >  SRR19973061.573059/1‑150 (MQ=60)
                                                                                                                                                     AGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTT  >  SRR19973061.3237484/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTT  >  CP009273/459176‑459474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑