Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,322,140 T→A T159S (ACG→TCG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,322,1400TA100.0% 714.5 / NA 212T159S (ACG→TCG) btuRcob(I)yrinic acid a,c‑diamide adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (100/112);  total (100/112)

Only 100 of 213 total aligned reads displayed.

TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGT  >  CP009273/1321993‑1322285
                                                                                                                                                   |                                                                                                                                                 
 gCCGGACGTCATATCCGGCATTTTTACAGATTAGTAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGAt                                                                                                                                                <  2:806289/150‑1 (MQ=255)
 gCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGAt                                                                                                                                                <  2:3335576/150‑1 (MQ=255)
  ccGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATa                                                                                                                                               <  2:2427437/150‑1 (MQ=255)
  ccGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATa                                                                                                                                               >  1:1723579/1‑150 (MQ=255)
    ggACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAAt                                                                                                                                             >  2:3344775/1‑150 (MQ=255)
    ggACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAAt                                                                                                                                             >  1:1744232/1‑150 (MQ=255)
     gACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATc                                                                                                                                            >  2:3674863/1‑150 (MQ=255)
     gACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATc                                                                                                                                            <  2:1877052/150‑1 (MQ=255)
     gACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATc                                                                                                                                            <  1:3304819/150‑1 (MQ=255)
        gTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCAcc                                                                                                                                         >  2:1546837/1‑150 (MQ=255)
           atatCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTc                                                                                                                                      <  1:965070/150‑1 (MQ=255)
             atCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTg                                                                                                                                    <  1:1170296/150‑1 (MQ=255)
              tCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGt                                                                                                                                   <  2:1161684/150‑1 (MQ=255)
                   cATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtg                                                                                                                              >  2:2549044/1‑150 (MQ=255)
                    aTTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtgt                                                                                                                             <  1:2660722/150‑1 (MQ=255)
                    aTTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtgt                                                                                                                             >  2:1435317/1‑150 (MQ=255)
                      ttttACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTGACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTgg                                                                                                                           <  2:3236434/150‑1 (MQ=255)
                            aGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTc                                                                                                                     <  2:1985360/150‑1 (MQ=255)
                            aGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTc                                                                                                                     >  1:2106157/1‑150 (MQ=255)
                            aGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTc                                                                                                                     <  2:2960446/150‑1 (MQ=255)
                             gATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCa                                                                                                                    >  1:2311053/1‑150 (MQ=255)
                                taataaTCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAttt                                                                                                                 >  1:3180622/1‑150 (MQ=255)
                                 aataatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTa                                                                                                                <  2:1362478/150‑1 (MQ=255)
                                  ataatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTaa                                                                                                               >  2:1061221/1‑150 (MQ=255)
                                   taatCGATCACTATCTGCGTTTTTACACCGGCATTATACGCTTGTTTGATGGGGTGTAATTTATTTACCGTATCTGCCAGTTCAAGAATATCACGATGACAACCACGACCATAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAAc                                                                                                              <  1:1116864/150‑1 (MQ=255)
                                     atCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGc                                                                                                            >  1:3514706/1‑150 (MQ=255)
                                       cGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCt                                                                                                          >  2:1309944/1‑150 (MQ=255)
                                       cGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCt                                                                                                          >  1:2495968/1‑150 (MQ=255)
                                        gATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTg                                                                                                         >  2:2723700/1‑150 (MQ=255)
                                        gATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTg                                                                                                         >  1:3698640/1‑150 (MQ=255)
                                           cccTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcac                                                                                                      >  1:3201685/1‑150 (MQ=255)
                                           cccTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcac                                                                                                      >  1:2643731/1‑150 (MQ=255)
                                               aTCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCAct                                                                                                  <  2:2106731/150‑1 (MQ=255)
                                               aTCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCAct                                                                                                  >  1:3294602/1‑150 (MQ=255)
                                               aTCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCAct                                                                                                  <  2:1397626/150‑1 (MQ=255)
                                                tCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActt                                                                                                 <  1:438613/150‑1 (MQ=255)
                                                 cTGCGCTTTTACACCGGCATCAAACGCATGTTTTACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtc                                                                                                >  1:2131753/1‑150 (MQ=255)
                                                  tGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtct                                                                                               <  2:1167655/150‑1 (MQ=255)
                                                   gcgcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtctt                                                                                              <  1:3715291/150‑1 (MQ=255)
                                                   gcgcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtctt                                                                                              <  1:612677/150‑1 (MQ=255)
                                                     gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                            >  1:2334734/1‑150 (MQ=255)
                                                      cTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCa                                                                                           >  2:3527382/1‑150 (MQ=255)
                                                         ttACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTGACGCCTGCACCACTTCTTCCAGTg                                                                                        <  2:2780938/150‑1 (MQ=255)
                                                          tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTTCCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                       <  2:499836/150‑1 (MQ=255)
                                                                  cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                               >  1:89176/1‑150 (MQ=255)
                                                                   aTCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTc                                                                              <  1:2472812/150‑1 (MQ=255)
                                                                   aTCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTc                                                                              <  1:2723700/150‑1 (MQ=255)
                                                                     cAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCAt                                                                            <  1:2947693/150‑1 (MQ=255)
                                                                      aaaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATa                                                                           <  2:29277/150‑1 (MQ=255)
                                                                      aaaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATa                                                                           <  2:1463033/150‑1 (MQ=255)
                                                                        aCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACg                                                                         >  2:2035293/1‑150 (MQ=255)
                                                                        aCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACg                                                                         <  2:851583/150‑1 (MQ=255)
                                                                         cGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGc                                                                        <  2:1856806/150‑1 (MQ=255)
                                                                           cATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGcca                                                                      <  1:594343/150‑1 (MQ=255)
                                                                                   aCGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATAc                                                              <  2:3201053/150‑1 (MQ=255)
                                                                                    cGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACg                                                             <  2:133468/150‑1 (MQ=255)
                                                                                      gggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTc                                                           <  1:3103413/150‑1 (MQ=255)
                                                                                         cGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGt                                                        <  2:3792998/150‑1 (MQ=255)
                                                                                          gTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGtt                                                       <  1:2993762/150‑1 (MQ=255)
                                                                                           tAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtc                                                      <  2:3227696/150‑1 (MQ=255)
                                                                                            aaTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtca                                                     >  1:1540056/1‑150 (MQ=255)
                                                                                             aTTCACTTAGCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcat                                                    >  1:1376750/1‑150 (MQ=255)
                                                                                               tCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcatca                                                  <  2:3019907/150‑1 (MQ=255)
                                                                                                 aCTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAg                                                <  1:690030/150‑1 (MQ=255)
                                                                                                     aCCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaaa                                            >  1:2200873/1‑150 (MQ=255)
                                                                                                       cGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc                                          <  1:1518807/150‑1 (MQ=255)
                                                                                                       cGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc                                          <  1:1594633/150‑1 (MQ=255)
                                                                                                        gTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAcc                                         >  2:2800444/1‑150 (MQ=255)
                                                                                                        gTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAcc                                         <  2:3062110/150‑1 (MQ=255)
                                                                                                              gCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc                                   <  1:1435317/150‑1 (MQ=255)
                                                                                                              gCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATca                                   <  1:1311571/150‑2 (MQ=255)
                                                                                                               ccAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCa                                  <  2:1771017/150‑1 (MQ=255)
                                                                                                               ccAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCa                                  >  2:2362566/1‑150 (MQ=255)
                                                                                                                cAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAg                                 >  2:2306106/1‑150 (MQ=255)
                                                                                                                cAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAg                                 <  1:205731/150‑1 (MQ=255)
                                                                                                                   ttCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGa                              <  1:3474413/150‑1 (MQ=255)
                                                                                                                   ttCAAGAATATCCCGATGACAACCAAGACACGAGATAATCACCGTATGTTGATGTGGAAGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGa                              <  1:2501066/150‑1 (MQ=255)
                                                                                                                    tCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAg                             >  1:1024033/1‑150 (MQ=255)
                                                                                                                    tCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAg                             <  1:3777059/150‑1 (MQ=255)
                                                                                                                         aaTATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATc                        <  2:1686719/150‑1 (MQ=255)
                                                                                                                         aaTATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATc                        >  1:522685/1‑150 (MQ=255)
                                                                                                                          atatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCa                       <  2:2335347/150‑1 (MQ=255)
                                                                                                                           tatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCag                      >  2:2610395/1‑150 (MQ=255)
                                                                                                                           tatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCag                      >  2:1521040/1‑150 (MQ=255)
                                                                                                                            atCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagc                     >  2:876940/1‑150 (MQ=255)
                                                                                                                                cGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaagc                 >  1:2521393/1‑150 (MQ=255)
                                                                                                                                   tGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATc              >  2:3116014/1‑150 (MQ=255)
                                                                                                                                   tGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATc              >  2:3255071/1‑150 (MQ=255)
                                                                                                                                   tGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATc              >  1:2460037/1‑150 (MQ=255)
                                                                                                                                    gACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATAACCAGTGAGGAATCAGCAAGCATcc             >  2:3470767/1‑150 (MQ=255)
                                                                                                                                    gACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACATCTTCCAGTGGCAAATAGTCATACGCCACCATATACTTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATcc             >  1:641438/1‑150 (MQ=255)
                                                                                                                                      cAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGc           >  2:2161439/1‑150 (MQ=255)
                                                                                                                                      cAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGc           >  2:2025326/1‑150 (MQ=255)
                                                                                                                                       aaCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt          >  1:2433926/1‑150 (MQ=255)
                                                                                                                                        aCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCtt         <  2:3298010/150‑1 (MQ=255)
                                                                                                                                        aCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCtt         <  2:10961/150‑1 (MQ=255)
                                                                                                                                        aCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCtt         <  1:3344775/150‑1 (MQ=255)
                                                                                                                                          cACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTg       <  1:2549044/150‑1 (MQ=255)
                                                                                                                                             gACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt    >  2:2481088/1‑150 (MQ=255)
                                                                                                                                             gACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt    <  1:1710370/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGT  >  CP009273/1321993‑1322285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGT  >  CP009273/1321993‑1322285
                                                                                                                                                   |                                                                                                                                                 
TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGA                                                                                                                                                 >  SRR19973061.3306501/1‑150 (MQ=60)
 GCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGAT                                                                                                                                                <  SRR19973061.1439394/150‑1 (MQ=60)
  CCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATA                                                                                                                                               <  SRR19973061.2082815/150‑1 (MQ=60)
  CCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATA                                                                                                                                               >  SRR19973061.1727902/1‑150 (MQ=60)
    GGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAAT                                                                                                                                             >  SRR19973061.1748614/1‑150 (MQ=60)
     GACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATC                                                                                                                                            >  SRR19973061.2112018/1‑150 (MQ=60)
     GACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATC                                                                                                                                            <  SRR19973061.3313336/150‑1 (MQ=60)
     GACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATC                                                                                                                                            <  SRR19973061.3313359/150‑1 (MQ=60)
      ACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGGTGACAACCACGACCCGAGATAATCA                                                                                                                                           <  SRR19973061.2892672/150‑1 (MQ=60)
         TCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCG                                                                                                                                        <  SRR19973061.2457585/150‑1 (MQ=60)
          CATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGT                                                                                                                                       <  SRR19973061.3743788/150‑1 (MQ=60)
           ATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTC                                                                                                                                      <  SRR19973061.967506/150‑1 (MQ=60)
             ATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTG                                                                                                                                    <  SRR19973061.1173258/150‑1 (MQ=60)
                  GCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAT                                                                                                                               <  SRR19973061.3036915/150‑1 (MQ=60)
                    ATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGT                                                                                                                             <  SRR19973061.2667560/150‑1 (MQ=60)
                            AGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTC                                                                                                                     >  SRR19973061.2111442/1‑150 (MQ=60)
                             GATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCA                                                                                                                    >  SRR19973061.2316912/1‑150 (MQ=60)
                               TTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTGACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATT                                                                                                                  <  SRR19973061.3122203/150‑1 (MQ=60)
                                TAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTT                                                                                                                 >  SRR19973061.3188782/1‑150 (MQ=60)
                                TAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTT                                                                                                                 >  SRR19973061.3188770/1‑150 (MQ=60)
                                 AATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTA                                                                                                                <  SRR19973061.959166/150‑1 (MQ=60)
                                   TAATCGATCACTATCTGCGTTTTTACACCGGCATTATACGCTTGTTTGATGGGGTGTAATTTATTTACCGTATCTGCCAGTTCAAGAATATCACGATGACAACCACGACCATAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAAC                                                                                                              <  SRR19973061.1119720/150‑1 (MQ=60)
                                    AATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACG                                                                                                             <  SRR19973061.2178054/150‑1 (MQ=60)
                                     ATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGC                                                                                                            >  SRR19973061.3523710/1‑150 (MQ=60)
                                       CGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCT                                                                                                          >  SRR19973061.2502324/1‑150 (MQ=60)
                                        GATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTG                                                                                                         >  SRR19973061.3708091/1‑150 (MQ=60)
                                        GATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTG                                                                                                         <  SRR19973061.2531864/150‑1 (MQ=60)
                                        GATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTG                                                                                                         >  SRR19973061.1761115/1‑150 (MQ=60)
                                           CCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCAC                                                                                                      >  SRR19973061.3209279/1‑150 (MQ=60)
                                           CCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCAC                                                                                                      >  SRR19973061.2650520/1‑150 (MQ=60)
                                           CCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCAC                                                                                                      >  SRR19973061.3209913/1‑150 (MQ=60)
                                             CTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCA                                                                                                    <  SRR19973061.1171622/150‑1 (MQ=60)
                                               ATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACT                                                                                                  >  SRR19973061.2504206/1‑150 (MQ=60)
                                               ATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACT                                                                                                  <  SRR19973061.3406190/150‑1 (MQ=60)
                                               ATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACT                                                                                                  >  SRR19973061.3303084/1‑150 (MQ=60)
                                                TCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTT                                                                                                 <  SRR19973061.439481/150‑1 (MQ=60)
                                                TCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTT                                                                                                 <  SRR19973061.439806/150‑1 (MQ=60)
                                                 CTGCGCTTTTACACCGGCATCAAACGCATGTTTTACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTC                                                                                                >  SRR19973061.2137108/1‑150 (MQ=60)
                                                  TGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCT                                                                                               >  SRR19973061.821150/1‑150 (MQ=60)
                                                  TGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCT                                                                                               >  SRR19973061.2674523/1‑150 (MQ=60)
                                                   GCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTT                                                                                              <  SRR19973061.614252/150‑1 (MQ=60)
                                                   GCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTT                                                                                              <  SRR19973061.3724788/150‑1 (MQ=60)
                                                   GCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTT                                                                                              <  SRR19973061.614234/150‑1 (MQ=60)
                                                   GCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTT                                                                                              <  SRR19973061.3724761/150‑1 (MQ=60)
                                                    CGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTC                                                                                             >  SRR19973061.880660/1‑150 (MQ=60)
                                                     GCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCC                                                                                            >  SRR19973061.2340662/1‑150 (MQ=60)
                                                       TTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAG                                                                                          >  SRR19973061.266001/1‑150 (MQ=60)
                                                                  CATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGT                                                                               >  SRR19973061.89476/1‑150 (MQ=60)
                                                                   ATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTC                                                                              <  SRR19973061.2730708/150‑1 (MQ=60)
                                                                   ATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTC                                                                              <  SRR19973061.2479110/150‑1 (MQ=60)
                                                                   ATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTC                                                                              <  SRR19973061.2478985/150‑1 (MQ=60)
                                                                     CAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCAT                                                                            <  SRR19973061.2955286/150‑1 (MQ=60)
                                                                        ACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACG                                                                         >  SRR19973061.1580089/1‑150 (MQ=60)
                                                                           CATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCA                                                                      <  SRR19973061.595893/150‑1 (MQ=60)
                                                                                TTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATA                                                                 <  SRR19973061.639718/150‑1 (MQ=60)
                                                                                TTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATA                                                                 <  SRR19973061.2317453/150‑1 (MQ=60)
                                                                                 TGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATAT                                                                >  SRR19973061.2494897/1‑150 (MQ=60)
                                                                                 TGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATAT                                                                >  SRR19973061.1230163/1‑150 (MQ=60)
                                                                                     GGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGT                                                            <  SRR19973061.3684255/150‑1 (MQ=60)
                                                                                      GGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTC                                                           <  SRR19973061.3111409/150‑1 (MQ=60)
                                                                                          GTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTT                                                       <  SRR19973061.3001488/150‑1 (MQ=60)
                                                                                            AATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCA                                                     >  SRR19973061.3722567/1‑150 (MQ=60)
                                                                                            AATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCA                                                     >  SRR19973061.2636116/1‑150 (MQ=60)
                                                                                            AATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCA                                                     >  SRR19973061.498220/1‑150 (MQ=60)
                                                                                            AATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCA                                                     >  SRR19973061.1543876/1‑150 (MQ=60)
                                                                                            AATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCA                                                     >  SRR19973061.1543891/1‑150 (MQ=60)
                                                                                             ATTCACTTAGCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCAT                                                    >  SRR19973061.1380227/1‑150 (MQ=60)
                                                                                                 ACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAG                                                <  SRR19973061.691819/150‑1 (MQ=60)
                                                                                                     ACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAA                                            >  SRR19973061.2206403/1‑150 (MQ=60)
                                                                                                      CCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAA                                           >  SRR19973061.649074/1‑150 (MQ=60)
                                                                                                       CGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAC                                          <  SRR19973061.1598591/150‑1 (MQ=60)
                                                                                                       CGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAC                                          <  SRR19973061.1748470/150‑1 (MQ=60)
                                                                                                       CGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAC                                          <  SRR19973061.1522603/150‑1 (MQ=60)
                                                                                                             TGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATC                                    >  SRR19973061.2434205/1‑150 (MQ=60)
                                                                                                              GCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCC                                   <  SRR19973061.1665345/150‑1 (MQ=60)
                                                                                                              GCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCC                                   <  SRR19973061.1438939/150‑1 (MQ=60)
                                                                                                              GCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCA                                   <  SRR19973061.1314857/150‑1 (MQ=60)
                                                                                                                CAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAG                                 <  SRR19973061.1550683/150‑1 (MQ=60)
                                                                                                                CAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAG                                 <  SRR19973061.206345/150‑1 (MQ=60)
                                                                                                                   TTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGA                              >  SRR19973061.1372596/1‑150 (MQ=60)
                                                                                                                   TTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGA                              <  SRR19973061.3483306/150‑1 (MQ=60)
                                                                                                                   TTCAAGAATATCCCGATGACAACCAAGACACGAGATAATCACCGTATGTTGATGTGGAAGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGA                              <  SRR19973061.2507434/150‑1 (MQ=60)
                                                                                                                    TCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAG                             <  SRR19973061.3786700/150‑1 (MQ=60)
                                                                                                                    TCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAG                             >  SRR19973061.1026648/1‑150 (MQ=60)
                                                                                                                       AGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAA                          <  SRR19973061.3301422/150‑1 (MQ=60)
                                                                                                                         AATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATC                        >  SRR19973061.524064/1‑150 (MQ=60)
                                                                                                                              CCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATAAACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAA                   >  SRR19973061.2956825/1‑150 (MQ=60)
                                                                                                                                CGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGC                 >  SRR19973061.2527813/1‑150 (MQ=60)
                                                                                                                                CGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGC                 >  SRR19973061.2527390/1‑150 (MQ=60)
                                                                                                                                CGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGC                 >  SRR19973061.1398972/1‑150 (MQ=60)
                                                                                                                                CGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGC                 >  SRR19973061.532243/1‑150 (MQ=60)
                                                                                                                                  ATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCAT               <  SRR19973061.1063948/150‑1 (MQ=60)
                                                                                                                                   TGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATC              >  SRR19973061.2466302/1‑150 (MQ=60)
                                                                                                                                    GACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCC             >  SRR19973061.2553666/1‑150 (MQ=60)
                                                                                                                                    GACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACATCTTCCAGTGGCAAATAGTCATACGCCACCATATACTTCAGTTCATCAAGCAAAACCATATCCAGTGAGAAATCAGCAATCATCC             >  SRR19973061.643075/1‑150 (MQ=60)
                                                                                                                                       AACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCT          >  SRR19973061.2440136/1‑150 (MQ=60)
                                                                                                                                        ACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTT         <  SRR19973061.3484980/150‑1 (MQ=60)
                                                                                                                                        ACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTT         <  SRR19973061.3353385/150‑1 (MQ=60)
                                                                                                                                          CACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTG       <  SRR19973061.2555531/150‑1 (MQ=60)
                                                                                                                                             GACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAT    <  SRR19973061.1714776/150‑1 (MQ=60)
                                                                                                                                             GACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAT    <  SRR19973061.1714665/150‑1 (MQ=60)
                                                                                                                                               CCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGT  >  SRR19973061.2248984/1‑150 (MQ=60)
                                                                                                                                               CCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGT  >  SRR19973061.2248452/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                 
TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGT  >  CP009273/1321993‑1322285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑