Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,322,140 T→A 100% T159S (ACG→TCG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,322,1400TA98.4% 804.8 / 1.6 242T159S (ACG→TCG) btuRcob(I)yrinic acid a,c‑diamide adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (130/108);  total (131/111)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 246 total aligned reads displayed.

TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTT  >  CP009273/1321993‑1322286
                                                                                                                                                   |                                                                                                                                                  
tGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCgaga                                                                                                                                                  >  1:829950/1‑150 (MQ=255)
   cGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATaa                                                                                                                                               >  1:3634599/1‑150 (MQ=255)
            tatCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCt                                                                                                                                      <  2:4141126/150‑1 (MQ=255)
            tatCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCt                                                                                                                                      <  1:4675819/150‑1 (MQ=255)
              tCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGt                                                                                                                                    >  1:3180021/1‑150 (MQ=255)
                cGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTg                                                                                                                                  >  2:3049218/1‑150 (MQ=255)
                   cATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACGTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtg                                                                                                                               <  2:221190/150‑1 (MQ=255)
                     tttttACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtgtg                                                                                                                             <  1:702702/150‑1 (MQ=255)
                          aCAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGt                                                                                                                        <  2:2537202/150‑1 (MQ=255)
                          aCAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGt                                                                                                                        >  1:599749/1‑150 (MQ=255)
                              aTTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAt                                                                                                                    <  1:3070370/150‑1 (MQ=255)
                               ttAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAtt                                                                                                                   >  2:1665454/1‑150 (MQ=255)
                               ttAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAtt                                                                                                                   >  1:1147006/1‑150 (MQ=255)
                                  ataatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTaa                                                                                                                <  1:1973142/150‑1 (MQ=255)
                                    aatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACg                                                                                                              >  1:1312409/1‑150 (MQ=255)
                                      tCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGcc                                                                                                            >  2:1516093/1‑150 (MQ=255)
                                       cGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCt                                                                                                           <  1:3317839/150‑1 (MQ=255)
                                       cGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCt                                                                                                           >  2:4524512/1‑150 (MQ=255)
                                        gATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTg                                                                                                          <  2:3622889/150‑1 (MQ=255)
                                          tCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGca                                                                                                        >  2:3751335/1‑150 (MQ=255)
                                          tCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGca                                                                                                        >  2:2934918/1‑150 (MQ=255)
                                          tCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGATGATGTGGACGTTCATTTAACGCCTGca                                                                                                        >  2:2354801/1‑150 (MQ=255)
                                           cccTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcac                                                                                                       >  2:4905170/1‑150 (MQ=255)
                                            ccTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcacc                                                                                                      >  2:1076397/1‑150 (MQ=255)
                                             ttATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcacca                                                                                                     >  2:3320812/2‑150 (MQ=255)
                                             ttATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcacca                                                                                                     >  2:4631870/2‑150 (MQ=255)
                                             cTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcacca                                                                                                     >  1:1323859/1‑150 (MQ=255)
                                               aTCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCAct                                                                                                   <  1:3191088/150‑1 (MQ=255)
                                               aTCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCAct                                                                                                   <  1:1395871/150‑1 (MQ=255)
                                                 cTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtc                                                                                                 >  1:1015442/1‑150 (MQ=255)
                                                  tGCGCTTTTACACCGGCATCAAACGCATGGTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATCTCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtct                                                                                                >  2:68233/1‑150 (MQ=255)
                                                  tGCGCTTGTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtct                                                                                                <  2:59355/150‑1 (MQ=255)
                                                     gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                             <  1:41365/150‑1 (MQ=255)
                                                      cTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCa                                                                                            <  1:2038311/150‑1 (MQ=255)
                                                         ttACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTg                                                                                         <  2:4336447/150‑1 (MQ=255)
                                                          tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                        >  1:3051559/1‑150 (MQ=255)
                                                          tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                        >  1:2229208/1‑150 (MQ=255)
                                                              ccGGCATCAAACTCATGTTTGACGGGGCTTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCaaa                                                                                    <  1:2795093/150‑1 (MQ=255)
                                                                 gCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATCTCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAg                                                                                 <  1:4903778/150‑1 (MQ=255)
                                                                  cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                                <  2:2828759/150‑1 (MQ=255)
                                                                  cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                                >  1:154245/1‑150 (MQ=255)
                                                                  cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                                <  2:3284352/150‑1 (MQ=255)
                                                                  cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                                <  1:2762840/150‑1 (MQ=255)
                                                                       aaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATAc                                                                           >  1:2419147/1‑150 (MQ=255)
                                                                       aaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATAc                                                                           >  2:4720431/1‑150 (MQ=255)
                                                                       aaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATAc                                                                           >  2:2199455/1‑150 (MQ=255)
                                                                        aCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACg                                                                          <  1:4788218/150‑1 (MQ=255)
                                                                         cGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGc                                                                         >  2:4250991/1‑150 (MQ=255)
                                                                            aTGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGccac                                                                      >  1:4982111/1‑150 (MQ=255)
                                                                             tGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGccacc                                                                     <  2:2163373/150‑1 (MQ=255)
                                                                             tGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGccacc                                                                     >  1:2861854/1‑150 (MQ=255)
                                                                               tttGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGACTATCCCGATGACACCCCCGACCCGAGATAATCACCGTCTGTTGATGTGGACGGTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCat                                                                   <  2:4279672/150‑1 (MQ=255)
                                                                               tttGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCat                                                                   <  1:3861982/150‑1 (MQ=255)
                                                                                 tGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCatat                                                                 <  1:429284/150‑1 (MQ=255)
                                                                                 tGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCatat                                                                 >  1:4078311/1‑150 (MQ=255)
                                                                                  gACGGGGCGTAATTCACATACCGTATCTGCCAGTTCAAGAATATCCCGAAGAAAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTAATTTAACTACTGCACCACTTCTTACAGTGGCAAATAGTCATACGCCACCAtata                                                                >  1:1715455/1‑150 (MQ=255)
                                                                                   aCGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATAc                                                               <  2:4806821/150‑1 (MQ=255)
                                                                                      gggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTc                                                            <  1:2286954/150‑1 (MQ=255)
                                                                                      gggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGGTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTc                                                            >  1:3165156/1‑150 (MQ=255)
                                                                                       ggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCa                                                           <  2:2441593/150‑1 (MQ=255)
                                                                                       ggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCa                                                           <  1:3277144/150‑1 (MQ=255)
                                                                                        gCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAg                                                          >  2:562169/1‑150 (MQ=255)
                                                                                             aTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcat                                                     <  1:3551853/150‑1 (MQ=255)
                                                                                             aTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcat                                                     <  1:1790590/150‑1 (MQ=255)
                                                                                             aTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcat                                                     >  1:3892683/1‑150 (MQ=255)
                                                                                                  cTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGc                                                >  1:1428364/1‑150 (MQ=255)
                                                                                                   ttACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCa                                               >  2:4639459/1‑150 (MQ=255)
                                                                                                      ccGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaaaa                                            <  2:812595/150‑1 (MQ=255)
                                                                                                      ccGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaaaa                                            >  2:2244459/1‑150 (MQ=255)
                                                                                                      ccGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaaaa                                            <  1:3793503/150‑1 (MQ=255)
                                                                                                       cGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc                                           >  1:1211830/1‑150 (MQ=255)
                                                                                                       cGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc                                           >  1:4024703/1‑150 (MQ=255)
                                                                                                          aTCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCat                                        <  2:2900108/150‑1 (MQ=255)
                                                                                                          aTCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCat                                        >  1:483890/1‑150 (MQ=255)
                                                                                                              gCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc                                    <  2:4711679/150‑1 (MQ=255)
                                                                                                               ccAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCa                                   >  1:1659924/1‑150 (MQ=255)
                                                                                                                cAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAg                                  >  2:1928951/1‑150 (MQ=255)
                                                                                                                cAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAg                                  >  1:4187997/1‑150 (MQ=255)
                                                                                                                     cAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAgg                             >  2:3545520/1‑150 (MQ=255)
                                                                                                                     cAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGATCATGTAACGCCTGCACCACTTCTTCCAGAGGCAAATAGTCATACGCCCCCATATACGTCAGATCATCAAGCAAAACCATATCCAGTGAgg                             >  1:912882/1‑150 (MQ=255)
                                                                                                                       aGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGaa                           >  2:1823042/1‑150 (MQ=255)
                                                                                                                       aGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGaa                           <  1:4724707/150‑1 (MQ=255)
                                                                                                                          atatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCa                        >  1:4890474/1‑150 (MQ=255)
                                                                                                                           tatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCag                       <  2:1015442/150‑1 (MQ=255)
                                                                                                                           tatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTCCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCACAACCATATCCAGTGAGGACTCag                       >  2:3927266/1‑150 (MQ=255)
                                                                                                                              cccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaa                    >  1:1122937/1‑150 (MQ=255)
                                                                                                                               ccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaag                   <  1:3773950/150‑1 (MQ=255)
                                                                                                                               ccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaag                   <  2:2795744/150‑1 (MQ=255)
                                                                                                                                cGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaagc                  <  2:1244679/150‑1 (MQ=255)
                                                                                                                                cGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaagc                  <  1:1816152/150‑1 (MQ=255)
                                                                                                                                  aTGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCAt                <  2:1253798/150‑1 (MQ=255)
                                                                                                                                       aaCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATATTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCAAATCCAGTGAGGAATCAGCAAGCATCCGCt           >  1:2548563/1‑150 (MQ=255)
                                                                                                                                        aCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCCAGCATCCGcgt          >  2:2628344/1‑148 (MQ=255)
                                                                                                                                        aCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCtt          >  1:798382/1‑150 (MQ=255)
                                                                                                                                          cACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGCCTCAGCAAGCATCCGCTTTg        >  2:4794466/1‑150 (MQ=255)
                                                                                                                                          cACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTg        >  1:1344964/1‑150 (MQ=255)
                                                                                                                                          cACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTg        >  1:4953940/1‑150 (MQ=255)
                                                                                                                                            cGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCa      >  1:2904913/1‑150 (MQ=255)
                                                                                                                                            cGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCa      >  2:4942604/1‑150 (MQ=255)
                                                                                                                                              aCCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATg    <  2:1428364/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTT  >  CP009273/1321993‑1322286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCC  >  CP009273/1321992‑1322289
                                                                                                                                                    |                                                                                                                                                     
ATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAG                                                                                                                                                      >  SRR19973063.4822189/1‑150 (MQ=60)
 TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGA                                                                                                                                                     >  SRR19973063.832347/1‑150 (MQ=60)
 TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGA                                                                                                                                                     >  SRR19973063.832335/1‑150 (MQ=60)
 TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGA                                                                                                                                                     >  SRR19973063.832305/1‑150 (MQ=60)
    CGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAA                                                                                                                                                  >  SRR19973063.3646262/1‑150 (MQ=60)
             TATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCT                                                                                                                                         <  SRR19973063.4690732/150‑1 (MQ=60)
               TCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGT                                                                                                                                       >  SRR19973063.3190179/1‑150 (MQ=60)
               TCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGT                                                                                                                                       >  SRR19973063.3190247/1‑150 (MQ=60)
                CCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTT                                                                                                                                      >  SRR19973063.3339034/1‑150 (MQ=60)
                  GGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGA                                                                                                                                    >  SRR19973063.2675312/1‑150 (MQ=60)
                    CATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATG                                                                                                                                  >  SRR19973063.137611/1‑150 (MQ=60)
                      TTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTG                                                                                                                                <  SRR19973063.704770/150‑1 (MQ=60)
                         TTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGAC                                                                                                                             <  SRR19973063.4948363/150‑1 (MQ=60)
                           ACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGT                                                                                                                           >  SRR19973063.601513/1‑150 (MQ=60)
                              GATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCA                                                                                                                        >  SRR19973063.2804707/1‑150 (MQ=60)
                              GATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCA                                                                                                                        >  SRR19973063.2804732/1‑150 (MQ=60)
                               ATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAT                                                                                                                       <  SRR19973063.3080234/150‑1 (MQ=60)
                                TTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATT                                                                                                                      >  SRR19973063.1150471/1‑150 (MQ=60)
                                   ATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAA                                                                                                                   >  SRR19973063.416378/1‑150 (MQ=60)
                                   ATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAA                                                                                                                   >  SRR19973063.416350/1‑150 (MQ=60)
                                   ATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAA                                                                                                                   <  SRR19973063.1979311/150‑1 (MQ=60)
                                   ATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAA                                                                                                                   <  SRR19973063.1979255/150‑1 (MQ=60)
                                     AATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACG                                                                                                                 >  SRR19973063.479760/1‑150 (MQ=60)
                                     AATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACG                                                                                                                 >  SRR19973063.1316321/1‑150 (MQ=60)
                                        CGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCT                                                                                                              <  SRR19973063.3328441/150‑1 (MQ=60)
                                        CGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCT                                                                                                              <  SRR19973063.2213123/150‑1 (MQ=60)
                                              CTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCA                                                                                                        >  SRR19973063.1327818/1‑150 (MQ=60)
                                                ATCTGCGCTTTTACACCGGCATCAACCGAATGTTTGTCGGGGCGTAATTCACTTACCTTATCTGCCTGTTAAAGAATATCCCGATGACAACCACGACCCGAGATATTCACCGTCTGTTTATGTGGTCGTTCATTTAACGCCTGCACCACT                                                                                                      <  SRR19973063.3196888/150‑1 (MQ=60)
                                                ATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACT                                                                                                      <  SRR19973063.3201349/150‑1 (MQ=60)
                                                ATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACT                                                                                                      <  SRR19973063.1400092/150‑1 (MQ=60)
                                                  CTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTC                                                                                                    >  SRR19973063.1018501/1‑150 (MQ=60)
                                                  CTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTC                                                                                                    <  SRR19973063.5059648/150‑1 (MQ=60)
                                                      GCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCC                                                                                                <  SRR19973063.41525/150‑1 (MQ=60)
                                                      GCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCC                                                                                                <  SRR19973063.512558/150‑1 (MQ=60)
                                                       CTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCA                                                                                               <  SRR19973063.2044683/150‑1 (MQ=60)
                                                       CTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCA                                                                                               <  SRR19973063.2927752/150‑1 (MQ=60)
                                                       CTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCA                                                                                               <  SRR19973063.2141706/150‑1 (MQ=60)
                                                        TTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAG                                                                                              <  SRR19973063.4850622/150‑1 (MQ=60)
                                                          TTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTG                                                                                            <  SRR19973063.1137336/150‑1 (MQ=60)
                                                           TACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGG                                                                                           >  SRR19973063.3879316/1‑150 (MQ=60)
                                                           TACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGG                                                                                           >  SRR19973063.3061333/1‑150 (MQ=60)
                                                           TACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGG                                                                                           >  SRR19973063.2236294/1‑150 (MQ=60)
                                                           TACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGG                                                                                           >  SRR19973063.2236272/1‑150 (MQ=60)
                                                               CCGGCATCAAACTCATGTTTGACGGGGCTTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAA                                                                                       <  SRR19973063.2804081/150‑1 (MQ=60)
                                                                CGGCATCAAACGCATGATCCACGGGGCGTAATTCACTTACCGTATCTGCCAGTCCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAAT                                                                                      <  SRR19973063.1751475/150‑1 (MQ=60)
                                                                 GGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATA                                                                                     >  SRR19973063.3791275/1‑150 (MQ=60)
                                                                 GGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATA                                                                                     >  SRR19973063.907635/1‑150 (MQ=60)
                                                                 GGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATA                                                                                     <  SRR19973063.741153/150‑1 (MQ=60)
                                                                  GCATCAAACTCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATTACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAG                                                                                    <  SRR19973063.4994242/150‑1 (MQ=60)
                                                                  GCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATCTCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAG                                                                                    <  SRR19973063.4919611/150‑1 (MQ=60)
                                                                  GCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCAGCACCACTTATTCCAGTGGCAAATAA                                                                                    >  SRR19973063.1403443/1‑150 (MQ=60)
                                                                   CATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGT                                                                                   <  SRR19973063.2771745/150‑1 (MQ=60)
                                                                   CATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGT                                                                                   >  SRR19973063.154721/1‑150 (MQ=60)
                                                                   CATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGT                                                                                   >  SRR19973063.3179370/1‑150 (MQ=60)
                                                                   CATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGT                                                                                   <  SRR19973063.2771901/150‑1 (MQ=60)
                                                                   CATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGT                                                                                   >  SRR19973063.154646/1‑150 (MQ=60)
                                                                    ATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTC                                                                                  <  SRR19973063.1319226/150‑1 (MQ=60)
                                                                    ATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTC                                                                                  <  SRR19973063.1319177/150‑1 (MQ=60)
                                                                    ATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTC                                                                                  >  SRR19973063.3985994/1‑150 (MQ=60)
                                                                     TCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCA                                                                                 <  SRR19973063.1696003/150‑1 (MQ=60)
                                                                      CAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCAT                                                                                >  SRR19973063.4787058/1‑150 (MQ=60)
                                                                        AACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATAC                                                                              >  SRR19973063.1451343/1‑150 (MQ=60)
                                                                        AACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATAC                                                                              >  SRR19973063.2426881/1‑150 (MQ=60)
                                                                         ACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACG                                                                             <  SRR19973063.4803515/150‑1 (MQ=60)
                                                                            CATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCA                                                                          >  SRR19973063.2017686/1‑150 (MQ=60)
                                                                             ATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCAC                                                                         >  SRR19973063.977725/1‑150 (MQ=60)
                                                                             ATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCAC                                                                         >  SRR19973063.4998211/1‑150 (MQ=60)
                                                                             ATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCAC                                                                         >  SRR19973063.4140358/1‑150 (MQ=60)
                                                                             ATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCAC                                                                         >  SRR19973063.977545/1‑150 (MQ=60)
                                                                              TGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACC                                                                        >  SRR19973063.2871022/1‑150 (MQ=60)
                                                                                TTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCAT                                                                      <  SRR19973063.3874267/150‑1 (MQ=60)
                                                                                 TTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATA                                                                     >  SRR19973063.1309059/1‑150 (MQ=60)
                                                                                  TGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATAT                                                                    >  SRR19973063.4091375/1‑150 (MQ=60)
                                                                                  TGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATAT                                                                    <  SRR19973063.430479/150‑1 (MQ=60)
                                                                                   GACGGGGCGTAATTCACATACCGTATCTGCCAGTTCAAGAATATCCCGAAGAAAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTAATTTAACTACTGCACCACTTCTTACAGTGGCAAATAGTCATACGCCACCATATA                                                                   >  SRR19973063.1720692/1‑150 (MQ=60)
                                                                                    ACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATAC                                                                  >  SRR19973063.392019/1‑150 (MQ=60)
                                                                                     CGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACG                                                                 >  SRR19973063.1699770/1‑150 (MQ=60)
                                                                                       GGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTC                                                               <  SRR19973063.2294253/150‑1 (MQ=60)
                                                                                       GGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGGTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTC                                                               >  SRR19973063.3175343/1‑150 (MQ=60)
                                                                                        GGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCA                                                              <  SRR19973063.3287665/150‑1 (MQ=60)
                                                                                             AATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCA                                                         >  SRR19973063.3312647/1‑150 (MQ=60)
                                                                                              ATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCAT                                                        <  SRR19973063.4372794/150‑1 (MQ=60)
                                                                                              ATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCAT                                                        <  SRR19973063.1796130/150‑1 (MQ=60)
                                                                                              ATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCAT                                                        >  SRR19973063.3905065/1‑150 (MQ=60)
                                                                                              ATTCACTGACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCAT                                                        <  SRR19973063.3563236/150‑1 (MQ=60)
                                                                                                TCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCA                                                      <  SRR19973063.1601261/150‑1 (MQ=60)
                                                                                                TCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCA                                                      <  SRR19973063.1188720/150‑1 (MQ=60)
                                                                                                   CTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCTCGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGC                                                   <  SRR19973063.2899724/150‑1 (MQ=60)
                                                                                                   CTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGC                                                   >  SRR19973063.1432719/1‑150 (MQ=60)
                                                                                                    TTACCGTATCTGCCATTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTAATTTAACGCCTGCTCCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCA                                                  <  SRR19973063.3284999/150‑1 (MQ=60)
                                                                                                      ACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCTAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGTCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAA                                                <  SRR19973063.1888572/150‑1 (MQ=60)
                                                                                                      ACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAA                                                <  SRR19973063.4467634/150‑1 (MQ=60)
                                                                                                       CCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAA                                               <  SRR19973063.3805703/150‑1 (MQ=60)
                                                                                                       CCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAA                                               <  SRR19973063.3805597/150‑1 (MQ=60)
                                                                                                       CCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAA                                               <  SRR19973063.3805598/150‑1 (MQ=60)
                                                                                                        CGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAC                                              >  SRR19973063.4037611/1‑150 (MQ=60)
                                                                                                        CGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAC                                              >  SRR19973063.1215466/1‑150 (MQ=60)
                                                                                                          TATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCA                                            <  SRR19973063.4858298/150‑1 (MQ=60)
                                                                                                           ATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCAT                                           >  SRR19973063.485270/1‑150 (MQ=60)
                                                                                                           ATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCAT                                           >  SRR19973063.3074468/1‑150 (MQ=60)
                                                                                                           ATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCAT                                           >  SRR19973063.334203/1‑150 (MQ=60)
                                                                                                                CCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCA                                      >  SRR19973063.1664983/1‑150 (MQ=60)
                                                                                                                CCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCA                                      <  SRR19973063.3723768/150‑1 (MQ=60)
                                                                                                                CCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCA                                      <  SRR19973063.4735483/150‑1 (MQ=60)
                                                                                                                 CAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAG                                     >  SRR19973063.4201371/1‑150 (MQ=60)
                                                                                                                     TCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAG                                 <  SRR19973063.2441473/150‑1 (MQ=60)
                                                                                                                      CAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGATCATGTAACGCCTGCACCACTTCTTCCAGAGGCAAATAGTCATACGCCCCCATATACGTCAGATCATCAAGCAAAACCATATCCAGTGAGG                                >  SRR19973063.915567/1‑150 (MQ=60)
                                                                                                                        AGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAA                              <  SRR19973063.4739769/150‑1 (MQ=60)
                                                                                                                         GAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAAT                             >  SRR19973063.679681/1‑150 (MQ=60)
                                                                                                                           ATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCA                           >  SRR19973063.4906183/1‑150 (MQ=60)
                                                                                                                           ATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCA                           >  SRR19973063.4906252/1‑150 (MQ=60)
                                                                                                                           ATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCA                           >  SRR19973063.4910391/1‑150 (MQ=60)
                                                                                                                            gagCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAG                          <  SRR19973063.2381065/147‑1 (MQ=60)
                                                                                                                            TATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAG                          <  SRR19973063.137584/150‑1 (MQ=60)
                                                                                                                               CCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAA                       >  SRR19973063.1126349/1‑150 (MQ=60)
                                                                                                                                CCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAG                      <  SRR19973063.3786002/150‑1 (MQ=60)
                                                                                                                                 CGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGC                     <  SRR19973063.1821796/150‑1 (MQ=60)
                                                                                                                                 CGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGC                     <  SRR19973063.1821696/150‑1 (MQ=60)
                                                                                                                                   ATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCAT                   <  SRR19973063.4039386/150‑1 (MQ=60)
                                                                                                                                    TGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATC                  >  SRR19973063.1056321/1‑150 (MQ=60)
                                                                                                                                      ACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCG                >  SRR19973063.2361062/1‑150 (MQ=60)
                                                                                                                                        AACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATATTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCAAATCCAGTGAGGAATCAGCAAGCATCCGCT              >  SRR19973063.2556688/1‑150 (MQ=60)
                                                                                                                                         ACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTT             <  SRR19973063.3135561/150‑1 (MQ=60)
                                                                                                                                         ACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTT             >  SRR19973063.800685/1‑150 (MQ=60)
                                                                                                                                          CCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTT            >  SRR19973063.421874/1‑150 (MQ=60)
                                                                                                                                          CCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGAGAGGAATCAGCAAGCATACGCTTT            >  SRR19973063.3027882/1‑150 (MQ=60)
                                                                                                                                           CACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTG           >  SRR19973063.4969959/1‑150 (MQ=60)
                                                                                                                                           CACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTG           >  SRR19973063.1348855/1‑150 (MQ=60)
                                                                                                                                           CACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTG           >  SRR19973063.1349004/1‑150 (MQ=60)
                                                                                                                                           CACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTG           <  SRR19973063.3581838/150‑1 (MQ=60)
                                                                                                                                            ACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCGTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCACGCATCCGCTGTGC          >  SRR19973063.3280076/1‑150 (MQ=60)
                                                                                                                                             CGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCA         >  SRR19973063.2914184/1‑150 (MQ=60)
                                                                                                                                             CGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCA         >  SRR19973063.1164118/1‑150 (MQ=60)
                                                                                                                                               ACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATG       <  SRR19973063.260365/150‑1 (MQ=60)
                                                                                                                                               ACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATG       <  SRR19973063.4960772/150‑1 (MQ=60)
                                                                                                                                                 CCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTT     >  SRR19973063.3664405/1‑150 (MQ=60)
                                                                                                                                                  CGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTG    >  SRR19973063.1687676/1‑150 (MQ=60)
                                                                                                                                                   GAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGC   >  SRR19973063.3699863/1‑150 (MQ=60)
                                                                                                                                                   GAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGC   >  SRR19973063.3699909/1‑150 (MQ=60)
                                                                                                                                                    AGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCC  <  SRR19973063.804304/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCC  >  CP009273/1321992‑1322289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑