Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 461,499 G→T 100% R424S (CGC→AGC)  ybaE ← putative ABC superfamily transporter periplasmic‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273461,4990GT98.7% 994.9 / 2.0 312R424S (CGC→AGC) ybaEputative ABC superfamily transporter periplasmic‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (156/152);  total (159/153)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 314 total aligned reads displayed.

CCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAC  >  CP009273/461352‑461643
                                                                                                                                                   |                                                                                                                                                
 cAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTc                                                                                                                                               >  1:358118/1‑150 (MQ=255)
 cAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTc                                                                                                                                               <  2:2570342/150‑1 (MQ=255)
      tataTTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                          >  2:5152435/1‑150 (MQ=255)
      tataTTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                          >  2:3680849/1‑150 (MQ=255)
       atatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCAt                                                                                                                                         <  2:3037742/150‑1 (MQ=255)
       agagtCCGGGGCTTCGCCAAATACTCTGTCGCCCATCTTGAGGGCTGCGTGTGCCTGTAGGGTCGTGTCGCCCCAGTTTATTGCGTTCTGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCAt                                                                                                                                         <  2:2970657/146‑1 (MQ=255)
          ttCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGt                                                                                                                                      <  1:1019298/150‑1 (MQ=255)
           tCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTa                                                                                                                                     >  2:3265695/1‑150 (MQ=255)
           tCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTa                                                                                                                                     >  2:3439479/1‑150 (MQ=255)
            ccGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTAt                                                                                                                                    >  1:1377620/1‑150 (MQ=255)
             cGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATg                                                                                                                                   <  1:4534/150‑1 (MQ=255)
             cGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGGTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATg                                                                                                                                   <  1:1762914/150‑1 (MQ=255)
              ggTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGa                                                                                                                                  >  1:2917549/1‑150 (MQ=255)
                tGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAg                                                                                                                                >  2:2159618/1‑150 (MQ=255)
                  cTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGtt                                                                                                                              <  1:3143161/150‑1 (MQ=255)
                  cTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGtt                                                                                                                              <  1:2517464/150‑1 (MQ=255)
                   ttCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTc                                                                                                                             >  2:1984420/1‑150 (MQ=255)
                     cGCCAATTACTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                           <  1:2622786/150‑1 (MQ=255)
                     cGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                           <  2:3537869/150‑1 (MQ=255)
                        cAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCg                                                                                                                        <  1:270050/150‑1 (MQ=255)
                         aaTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCgg                                                                                                                       >  2:1862139/1‑150 (MQ=255)
                          aTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGt                                                                                                                      >  2:1932852/1‑150 (MQ=255)
                           ttAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTa                                                                                                                     <  1:2871245/150‑1 (MQ=255)
                            tAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAg                                                                                                                    >  1:608871/1‑150 (MQ=255)
                            tAATCTGTCGCCCATCATCAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTCGGCTTTCTGCCATGGAATGAAGTTCTATCGGTAg                                                                                                                    >  2:3726860/1‑150 (MQ=255)
                                   tCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATaa                                                                                                             >  2:155758/1‑150 (MQ=255)
                                        catcatGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAg                                                                                                        >  2:1267316/1‑150 (MQ=255)
                                          tcatGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCg                                                                                                      <  1:3660716/150‑1 (MQ=255)
                                           catGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGt                                                                                                     >  1:130138/1‑150 (MQ=255)
                                             tGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCa                                                                                                   <  1:489870/150‑1 (MQ=255)
                                             tGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCATCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCa                                                                                                   >  2:3493056/1‑150 (MQ=255)
                                              gAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATACTTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCAGGGTCTGAAGTTCTATCGGTCGGTGATAAACCAGCGTCaa                                                                                                  >  2:2216161/1‑150 (MQ=255)
                                              gAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCaa                                                                                                  <  1:1076151/150‑1 (MQ=255)
                                              gAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCaa                                                                                                  >  2:437854/1‑150 (MQ=255)
                                              gAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCaa                                                                                                  <  2:32278/150‑1 (MQ=255)
                                              gAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTCTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCaa                                                                                                  >  2:3211462/1‑150 (MQ=255)
                                                 gTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGt                                                                                               >  1:3674604/1‑150 (MQ=255)
                                                    tGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGtttt                                                                                            >  2:762983/1‑150 (MQ=255)
                                                     gCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTc                                                                                           <  1:99607/150‑1 (MQ=255)
                                                      cGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCg                                                                                          <  2:4242320/150‑1 (MQ=255)
                                                         gtgCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTa                                                                                       <  2:3404383/150‑1 (MQ=255)
                                                          tgCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAg                                                                                      <  2:4475840/150‑1 (MQ=255)
                                                           gCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGt                                                                                     >  1:92897/1‑150 (MQ=255)
                                                             cTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGttt                                                                                   >  2:232928/1‑150 (MQ=255)
                                                              tGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTg                                                                                  <  2:1665569/150‑1 (MQ=255)
                                                               gTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGa                                                                                 >  2:60324/1‑150 (MQ=255)
                                                                  gggTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACtt                                                                              <  1:4337858/150‑1 (MQ=255)
                                                                          tcgtcCCAGTTTTTTTCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGt                                                                      <  1:4823774/150‑1 (MQ=255)
                                                                          tcgtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGt                                                                      >  2:2547720/1‑150 (MQ=255)
                                                                           cgtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTa                                                                     <  2:641213/150‑1 (MQ=255)
                                                                             tcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAcc                                                                   <  1:3439479/150‑1 (MQ=255)
                                                                              cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCt                                                                  <  1:2316541/150‑1 (MQ=255)
                                                                               ccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTg                                                                 <  2:1477618/150‑1 (MQ=255)
                                                                               ccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTg                                                                 >  2:3358514/1‑150 (MQ=255)
                                                                                 aGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGcc                                                               >  1:4572393/1‑150 (MQ=255)
                                                                                 aGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGcc                                                               <  2:3960390/150‑1 (MQ=255)
                                                                                  gTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCa                                                              <  1:2965576/150‑1 (MQ=255)
                                                                                  gTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCa                                                              >  2:4554437/1‑150 (MQ=255)
                                                                                     ttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATg                                                           <  2:4683388/150‑1 (MQ=255)
                                                                                       ttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCg                                                         <  1:3994433/150‑1 (MQ=255)
                                                                                          cGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGaa                                                      >  2:4157805/1‑150 (MQ=255)
                                                                                               tGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTc                                                 >  2:4936518/1‑150 (MQ=255)
                                                                                                gAAAAATAATTGTGAGTTCACAGCCGTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTcc                                                <  2:1175201/150‑1 (MQ=255)
                                                                                                  aaaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAg                                              >  2:4982570/1‑150 (MQ=255)
                                                                                                     ataatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCt                                           <  1:3807484/150‑1 (MQ=255)
                                                                                                         ttGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCa                                       >  1:3900468/1‑150 (MQ=255)
                                                                                                              aGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAAt                                  >  2:1611616/1‑150 (MQ=255)
                                                                                                               gTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATg                                 >  1:1523475/1‑150 (MQ=255)
                                                                                                                ttCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGc                                <  2:840205/150‑1 (MQ=255)
                                                                                                                ttCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGc                                <  1:1411479/150‑1 (MQ=255)
                                                                                                                 tCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCa                               <  1:2369673/150‑1 (MQ=255)
                                                                                                                   acaGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATg                             >  1:3205357/1‑150 (MQ=255)
                                                                                                                     aGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAc                           <  2:4888470/150‑1 (MQ=255)
                                                                                                                     aGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAc                           >  1:4453049/1‑150 (MQ=255)
                                                                                                                      gCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACt                          <  1:1153521/150‑1 (MQ=255)
                                                                                                                      gCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACt                          <  2:3025237/150‑1 (MQ=255)
                                                                                                                      gCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACt                          <  1:5052888/150‑1 (MQ=255)
                                                                                                                        cTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTgg                        >  1:1261847/1‑150 (MQ=255)
                                                                                                                         ttCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGc                       <  1:1984420/150‑1 (MQ=255)
                                                                                                                         ttCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGc                       >  2:753585/1‑150 (MQ=255)
                                                                                                                         ttCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGc                       >  2:1687914/1‑150 (MQ=255)
                                                                                                                         ttCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGc                       <  2:2057507/150‑1 (MQ=255)
                                                                                                                         ttCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGGAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGc                       <  2:4614191/150‑1 (MQ=255)
                                                                                                                           ccGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCgg                     >  1:4073534/1‑150 (MQ=255)
                                                                                                                            cGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGt                    <  1:2943008/150‑1 (MQ=255)
                                                                                                                              cTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGa                  >  1:226958/1‑150 (MQ=255)
                                                                                                                               tGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGAt                 <  1:4624951/150‑1 (MQ=255)
                                                                                                                                 ccAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCa               <  2:3860101/150‑1 (MQ=255)
                                                                                                                                   aGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTGGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAgg             <  2:717058/150‑1 (MQ=255)
                                                                                                                                     tgtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGtt           <  1:2046859/150‑1 (MQ=255)
                                                                                                                                       tCGCCTGTAGGCTTTCTTCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGtttt         <  1:1926887/150‑1 (MQ=255)
                                                                                                                                        cGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtc        <  2:2789865/150‑1 (MQ=255)
                                                                                                                                        cGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtc        <  1:762983/150‑1 (MQ=255)
                                                                                                                                        cGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtc        <  1:243291/150‑1 (MQ=255)
                                                                                                                                         gCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtct       >  1:4708898/1‑150 (MQ=255)
                                                                                                                                          ccTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtctc      >  1:4592589/1‑150 (MQ=255)
                                                                                                                                           cTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTcc     <  1:5090618/150‑1 (MQ=255)
                                                                                                                                            tGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCg    >  1:3128916/1‑150 (MQ=255)
                                                                                                                                             gTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGa   >  1:4208556/1‑150 (MQ=255)
                                                                                                                                              tAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACAAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCTTGACTGGCGGTGATCAGGTTTTCTCCGAc  <  1:1831111/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAC  >  CP009273/461352‑461643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTA  >  CP009273/461350‑461648
                                                                                                                                                     |                                                                                                                                                     
CTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCT                                                                                                                                                       >  SRR19973063.4555745/1‑150 (MQ=60)
 TCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTT                                                                                                                                                      <  SRR19973063.3819436/150‑1 (MQ=60)
 TCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTT                                                                                                                                                      >  SRR19973063.3174087/1‑150 (MQ=60)
   CAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTC                                                                                                                                                    >  SRR19973063.359148/1‑150 (MQ=60)
      AGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGC                                                                                                                                                 >  SRR19973063.1025034/1‑150 (MQ=60)
           CTTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGG                                                                                                                                            <  SRR19973063.2667659/150‑1 (MQ=60)
           ATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGG                                                                                                                                            >  SRR19973063.1193344/1‑150 (MQ=60)
            TTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGT                                                                                                                                           <  SRR19973063.1022371/150‑1 (MQ=60)
             TCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTA                                                                                                                                          >  SRR19973063.863525/1‑150 (MQ=60)
              CCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTAT                                                                                                                                         <  SRR19973063.2434692/150‑1 (MQ=60)
              CCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTAT                                                                                                                                         >  SRR19973063.1381774/1‑150 (MQ=60)
               CGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATG                                                                                                                                        <  SRR19973063.4558/150‑1 (MQ=60)
               CGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGGTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATG                                                                                                                                        <  SRR19973063.1768336/150‑1 (MQ=60)
                GGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGA                                                                                                                                       >  SRR19973063.2926854/1‑150 (MQ=60)
                    CTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTT                                                                                                                                   <  SRR19973063.3153267/150‑1 (MQ=60)
                    CTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTT                                                                                                                                   <  SRR19973063.2525490/150‑1 (MQ=60)
                    CTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTAGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGATGTT                                                                                                                                   <  SRR19973063.183854/150‑1 (MQ=60)
                       CGCCAATTACTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTA                                                                                                                                <  SRR19973063.2631139/150‑1 (MQ=60)
                       CGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTA                                                                                                                                <  SRR19973063.3517644/150‑1 (MQ=60)
                        GCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTAT                                                                                                                               <  SRR19973063.2989751/150‑1 (MQ=60)
                        GCCAATGAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCATTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACATCCTTCAGCTGCCAGTGTCGCCTGTAGTCTTTCTGCCATGGTATGAAGTTCTAT                                                                                                                               <  SRR19973063.3822716/150‑1 (MQ=60)
                          CAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCG                                                                                                                             <  SRR19973063.270852/150‑1 (MQ=60)
                             TTAATCTGTCGCCCATCTTGTGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCTTTATGAATAATAATTGTGAGTTCACAGCCTTCCGGTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTA                                                                                                                          <  SRR19973063.4887389/150‑1 (MQ=60)
                             TTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTA                                                                                                                          <  SRR19973063.2880436/150‑1 (MQ=60)
                              TAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAG                                                                                                                         >  SRR19973063.610660/1‑150 (MQ=60)
                              TAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAG                                                                                                                         >  SRR19973063.610699/1‑150 (MQ=60)
                              TAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAG                                                                                                                         >  SRR19973063.740450/1‑150 (MQ=60)
                              TAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAG                                                                                                                         >  SRR19973063.2822596/1‑150 (MQ=60)
                                ATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGT                                                                                                                       <  SRR19973063.3889940/150‑1 (MQ=60)
                                 TCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTG                                                                                                                      <  SRR19973063.1796758/150‑1 (MQ=60)
                                 TCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTG                                                                                                                      <  SRR19973063.44279/150‑1 (MQ=60)
                                  CTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGA                                                                                                                     >  SRR19973063.3146653/1‑150 (MQ=60)
                                  CTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGA                                                                                                                     >  SRR19973063.3146204/1‑150 (MQ=60)
                                  CTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGA                                                                                                                     >  SRR19973063.2675696/1‑150 (MQ=60)
                                   TGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGAT                                                                                                                    <  SRR19973063.958779/150‑1 (MQ=60)
                                   TGTCGCCCATCATGAGGTCTGAGTGTGCCTGTAGGGTCGTGTCTTCCCAGTTTTTTGCGTTATTCAAAATAATTGTGATTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGTCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGAT                                                                                                                    <  SRR19973063.958775/150‑1 (MQ=60)
                                    GTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATA                                                                                                                   >  SRR19973063.4551442/1‑150 (MQ=60)
                                     TCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAA                                                                                                                  >  SRR19973063.2782200/1‑150 (MQ=60)
                                       GCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAAC                                                                                                                >  SRR19973063.4085977/1‑150 (MQ=60)
                                        CCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACC                                                                                                               >  SRR19973063.1391870/1‑150 (MQ=60)
                                           ATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGC                                                                                                            <  SRR19973063.1959502/150‑1 (MQ=60)
                                            TCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCG                                                                                                           <  SRR19973063.3672453/150‑1 (MQ=60)
                                            TCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTAGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCG                                                                                                           >  SRR19973063.4490194/1‑150 (MQ=60)
                                             CATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGT                                                                                                          >  SRR19973063.4684678/1‑150 (MQ=60)
                                             CATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGT                                                                                                          >  SRR19973063.130555/1‑150 (MQ=60)
                                               TGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCA                                                                                                        <  SRR19973063.4626260/150‑1 (MQ=60)
                                               TGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCA                                                                                                        <  SRR19973063.491272/150‑1 (MQ=60)
                                                GAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAA                                                                                                       <  SRR19973063.1079417/150‑1 (MQ=60)
                                                  GGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGG                                                                                                     >  SRR19973063.4427334/1‑150 (MQ=60)
                                                  GGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGG                                                                                                     >  SRR19973063.2987825/1‑150 (MQ=60)
                                                   GTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGT                                                                                                    <  SRR19973063.2368825/150‑1 (MQ=60)
                                                   GTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGT                                                                                                    >  SRR19973063.4138184/1‑150 (MQ=60)
                                                   GTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGT                                                                                                    >  SRR19973063.3686096/1‑150 (MQ=60)
                                                   GTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGACAAGGT                                                                                                    >  SRR19973063.3686370/1‑150 (MQ=60)
                                                     CTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTT                                                                                                  <  SRR19973063.2956234/150‑1 (MQ=60)
                                                     CTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTT                                                                                                  <  SRR19973063.2166382/150‑1 (MQ=60)
                                                     CTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTT                                                                                                  <  SRR19973063.2072397/150‑1 (MQ=60)
                                                      TGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTT                                                                                                 <  SRR19973063.1842250/150‑1 (MQ=60)
                                                       GCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTC                                                                                                <  SRR19973063.99950/150‑1 (MQ=60)
                                                       GCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTC                                                                                                <  SRR19973063.100011/150‑1 (MQ=60)
                                                        CGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCG                                                                                               <  SRR19973063.2029449/150‑1 (MQ=60)
                                                        CGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCG                                                                                               <  SRR19973063.4595210/150‑1 (MQ=60)
                                                          TGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGT                                                                                             <  SRR19973063.1669617/150‑1 (MQ=60)
                                                             GCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGT                                                                                          >  SRR19973063.1777278/1‑150 (MQ=60)
                                                             GCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGT                                                                                          >  SRR19973063.93212/1‑150 (MQ=60)
                                                             GCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGT                                                                                          >  SRR19973063.93248/1‑150 (MQ=60)
                                                               CTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTT                                                                                        <  SRR19973063.2035776/150‑1 (MQ=60)
                                                                  TAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGAC                                                                                     >  SRR19973063.3699754/1‑150 (MQ=60)
                                                                    GGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTT                                                                                   <  SRR19973063.4351684/150‑1 (MQ=60)
                                                                     GGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTC                                                                                  >  SRR19973063.4990141/1‑150 (MQ=60)
                                                                     GGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTC                                                                                  >  SRR19973063.3650018/1‑150 (MQ=60)
                                                                     GGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTC                                                                                  >  SRR19973063.3605036/1‑150 (MQ=60)
                                                                          TGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCG                                                                             >  SRR19973063.1198507/1‑150 (MQ=60)
                                                                            TCGTCCCAGTTTTTTTCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGT                                                                           <  SRR19973063.4839220/150‑1 (MQ=60)
                                                                             CGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTA                                                                          <  SRR19973063.4249273/150‑1 (MQ=60)
                                                                              GTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAC                                                                         >  SRR19973063.3264113/1‑150 (MQ=60)
                                                                               TCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACC                                                                        <  SRR19973063.3450415/150‑1 (MQ=60)
                                                                               TCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACC                                                                        <  SRR19973063.3680377/150‑1 (MQ=60)
                                                                                CCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCT                                                                       <  SRR19973063.2323950/150‑1 (MQ=60)
                                                                                CCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCT                                                                       <  SRR19973063.2323941/150‑1 (MQ=60)
                                                                                   AGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCC                                                                    >  SRR19973063.4587417/1‑150 (MQ=60)
                                                                                   AGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCC                                                                    >  SRR19973063.4587021/1‑150 (MQ=60)
                                                                                    GTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCA                                                                   <  SRR19973063.2975057/150‑1 (MQ=60)
                                                                                    GTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCA                                                                   <  SRR19973063.2945002/150‑1 (MQ=60)
                                                                                     TTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAA                                                                  <  SRR19973063.603808/150‑1 (MQ=60)
                                                                                       TTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATG                                                                >  SRR19973063.1644825/1‑150 (MQ=60)
                                                                                        TTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGC                                                               >  SRR19973063.1093587/1‑150 (MQ=60)
                                                                                        TTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCATGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGAGTCAAGGTTATCGGTAGTTTGACTTCATACGTTACCTGCCAATGC                                                               >  SRR19973063.2622244/1‑150 (MQ=60)
                                                                                         TTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCG                                                              >  SRR19973063.2894803/1‑150 (MQ=60)
                                                                                         GTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCG                                                              <  SRR19973063.4007241/150‑1 (MQ=60)
                                                                                            CGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAA                                                           >  SRR19973063.2376932/1‑150 (MQ=60)
                                                                                            CGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAA                                                           >  SRR19973063.4554777/1‑150 (MQ=60)
                                                                                             GTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAAT                                                          >  SRR19973063.1053105/1‑150 (MQ=60)
                                                                                               TATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAG                                                        <  SRR19973063.2950483/150‑1 (MQ=60)
                                                                                               TATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAG                                                        <  SRR19973063.2950266/150‑1 (MQ=60)
                                                                                                   AAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCA                                                    <  SRR19973063.2905219/150‑1 (MQ=60)
                                                                                                    AAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAG                                                   <  SRR19973063.4204114/150‑1 (MQ=60)
                                                                                                      AATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCC                                                 >  SRR19973063.1477127/1‑150 (MQ=60)
                                                                                                      AATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCC                                                 >  SRR19973063.4066534/1‑150 (MQ=60)
                                                                                                       ATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCT                                                >  SRR19973063.4808203/1‑150 (MQ=60)
                                                                                                       ATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCT                                                <  SRR19973063.3819607/150‑1 (MQ=60)
                                                                                                        TAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGACTAGTCCAGCCTG                                               >  SRR19973063.247079/1‑150 (MQ=60)
                                                                                                         AATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGG                                              >  SRR19973063.1278407/1‑150 (MQ=60)
                                                                                                         AATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGG                                              >  SRR19973063.1278312/1‑150 (MQ=60)
                                                                                                          ATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCATCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGC                                             >  SRR19973063.2534826/1‑150 (MQ=60)
                                                                                                           TTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCA                                            >  SRR19973063.3912913/1‑150 (MQ=60)
                                                                                                           TTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCA                                            >  SRR19973063.3912891/1‑150 (MQ=60)
                                                                                                           TTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCA                                            >  SRR19973063.50738/1‑150 (MQ=60)
                                                                                                           TTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCA                                            <  SRR19973063.1399405/150‑1 (MQ=60)
                                                                                                              TGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTA                                         >  SRR19973063.137519/1‑150 (MQ=60)
                                                                                                                AGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAAT                                       >  SRR19973063.3203299/1‑150 (MQ=60)
                                                                                                                AGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAAT                                       >  SRR19973063.5070108/1‑150 (MQ=60)
                                                                                                                 GTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATG                                      >  SRR19973063.1418723/1‑150 (MQ=60)
                                                                                                                 GTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATG                                      <  SRR19973063.583379/150‑1 (MQ=60)
                                                                                                                 GTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATG                                      >  SRR19973063.1528146/1‑150 (MQ=60)
                                                                                                                  TTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGC                                     <  SRR19973063.1415760/150‑1 (MQ=60)
                                                                                                                   TCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCA                                    <  SRR19973063.2377263/150‑1 (MQ=60)
                                                                                                                     ACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATG                                  >  SRR19973063.295682/1‑150 (MQ=60)
                                                                                                                     ACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATG                                  >  SRR19973063.3215662/1‑150 (MQ=60)
                                                                                                                     ACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATG                                  >  SRR19973063.1358237/1‑150 (MQ=60)
                                                                                                                      CAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGA                                 >  SRR19973063.1086182/1‑150 (MQ=60)
                                                                                                                       AGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAC                                >  SRR19973063.4467340/1‑150 (MQ=60)
                                                                                                                       AGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAC                                >  SRR19973063.4467348/1‑150 (MQ=60)
                                                                                                                       AGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAC                                >  SRR19973063.2502148/1‑150 (MQ=60)
                                                                                                                        GCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACT                               <  SRR19973063.1157001/150‑1 (MQ=60)
                                                                                                                        GCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACT                               <  SRR19973063.5069158/150‑1 (MQ=60)
                                                                                                                          CTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGG                             >  SRR19973063.1265603/1‑150 (MQ=60)
                                                                                                                           TTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGC                            <  SRR19973063.1990621/150‑1 (MQ=60)
                                                                                                                            TCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCG                           <  SRR19973063.1715016/150‑1 (MQ=60)
                                                                                                                             CCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGG                          >  SRR19973063.4086586/1‑150 (MQ=60)
                                                                                                                              CGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCTATGCGGAATAGTCCGGCCTGGCAGTAATGCATGACTGGCGGT                         <  SRR19973063.4785619/150‑1 (MQ=60)
                                                                                                                              CGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGT                         <  SRR19973063.4309091/150‑1 (MQ=60)
                                                                                                                              CGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGT                         <  SRR19973063.2952407/150‑1 (MQ=60)
                                                                                                                              CGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGT                         >  SRR19973063.317773/1‑150 (MQ=60)
                                                                                                                              CGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGT                         <  SRR19973063.2339604/150‑1 (MQ=60)
                                                                                                                              CGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGT                         <  SRR19973063.2306094/150‑1 (MQ=60)
                                                                                                                                CTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGA                       >  SRR19973063.227637/1‑150 (MQ=60)
                                                                                                                                 TGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGAT                      <  SRR19973063.4639733/150‑1 (MQ=60)
                                                                                                                                 TGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGAT                      <  SRR19973063.4650036/150‑1 (MQ=60)
                                                                                                                                 TGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGAT                      <  SRR19973063.810477/150‑1 (MQ=60)
                                                                                                                                 TGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGCTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGAT                      <  SRR19973063.4639664/150‑1 (MQ=60)
                                                                                                                                     AGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATAGGTAGGTGATAAACCAGCGACAATGTAATCGGTAGTTTGACTTCATCCGGTACCTTCCAATGAGGAATAGTACAGACTGGCAGTAATGCATGACTTGCGGTGATCAGG                  >  SRR19973063.399590/1‑150 (MQ=60)
                                                                                                                                       TGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTT                <  SRR19973063.2053256/150‑1 (MQ=60)
                                                                                                                                         TCGCCTGTAGGCTTTCTTCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTT              <  SRR19973063.1932911/150‑1 (MQ=60)
                                                                                                                                         TCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTT              <  SRR19973063.1933023/150‑1 (MQ=60)
                                                                                                                                          CGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTC             <  SRR19973063.244028/150‑1 (MQ=60)
                                                                                                                                          CGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTC             <  SRR19973063.765213/150‑1 (MQ=60)
                                                                                                                                           GCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCT            >  SRR19973063.4725148/1‑150 (MQ=60)
                                                                                                                                           GCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCT            >  SRR19973063.4723901/1‑150 (MQ=60)
                                                                                                                                           GCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCT            <  SRR19973063.3618992/150‑1 (MQ=60)
                                                                                                                                            CCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTC           >  SRR19973063.4856950/1‑150 (MQ=60)
                                                                                                                                            CCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTC           >  SRR19973063.4607282/1‑150 (MQ=60)
                                                                                                                                             CTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCC          <  SRR19973063.5107003/150‑1 (MQ=60)
                                                                                                                                             CTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCC          >  SRR19973063.4247185/1‑150 (MQ=60)
                                                                                                                                             CTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCC          <  SRR19973063.239222/150‑1 (MQ=60)
                                                                                                                                             CTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCC          <  SRR19973063.3812881/150‑1 (MQ=60)
                                                                                                                                             CTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCGGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCC          <  SRR19973063.4998671/150‑1 (MQ=60)
                                                                                                                                              TGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCG         >  SRR19973063.3138987/1‑150 (MQ=60)
                                                                                                                                              TGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCG         <  SRR19973063.3457282/150‑1 (MQ=60)
                                                                                                                                               GTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGA        >  SRR19973063.4221980/1‑150 (MQ=60)
                                                                                                                                                TAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAC       <  SRR19973063.3339308/150‑1 (MQ=60)
                                                                                                                                                TAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACAAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCTTGACTGGCGGTGATCAGGTTTTCTCCGAC       <  SRR19973063.1836812/150‑1 (MQ=60)
                                                                                                                                                    CTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCT   <  SRR19973063.4451844/150‑1 (MQ=60)
                                                                                                                                                     TTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTA  <  SRR19973063.708794/150‑1 (MQ=60)
                                                                                                                                                     TTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTA  <  SRR19973063.708786/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTA  >  CP009273/461350‑461648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑