Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 518,820 C→A 100% A35E (GCG→GAG)  rhsD → Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273518,8200CA98.1% 678.4 / 2.6 211A35E (GCG→GAG) rhsDRhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (132/75);  total (134/77)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 231 total aligned reads displayed.

TTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGA  >  CP009273/518673‑518966
                                                                                                                                                   |                                                                                                                                                  
ttgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCggcgtggcgt                                                                                                                                                  >  1:977531/1‑150 (MQ=255)
ttgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCggcgtggagt                                                                                                                                                  <  2:335712/150‑1 (MQ=255)
ttgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAACGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCggcgtggagt                                                                                                                                                  <  2:4760524/150‑1 (MQ=255)
    tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTc                                                                                                                                              >  2:3049714/1‑150 (MQ=255)
      acacAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCgg                                                                                                                                            >  2:3232351/1‑150 (MQ=255)
        acaGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtg                                                                                                                                          >  2:2731847/1‑150 (MQ=255)
         caGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgt                                                                                                                                         <  2:2249702/150‑1 (MQ=255)
          aGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgtg                                                                                                                                        >  1:1108310/1‑150 (MQ=255)
          aGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgtg                                                                                                                                        >  1:174682/1‑150 (MQ=255)
               tatGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcgg                                                                                                                                   >  2:3622736/1‑150 (MQ=255)
               tatGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcgg                                                                                                                                   >  1:508583/1‑150 (MQ=255)
                atGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACCCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcggg                                                                                                                                  >  1:2256725/1‑150 (MQ=255)
                       aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATg                                                                                                                           >  2:145581/1‑150 (MQ=255)
                        tAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGa                                                                                                                          >  2:2140868/1‑150 (MQ=255)
                         aaCTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGAc                                                                                                                         >  2:924474/1‑150 (MQ=255)
                         aaCTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGAc                                                                                                                         >  2:3641330/1‑150 (MQ=255)
                             aaaaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCg                                                                                                                     <  1:466034/150‑1 (MQ=255)
                              aaaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCgg                                                                                                                    <  1:3878716/150‑1 (MQ=255)
                              aaaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCgg                                                                                                                    >  2:4549944/1‑150 (MQ=255)
                              aaaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCgg                                                                                                                    >  1:26858/1‑150 (MQ=255)
                               aaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCggg                                                                                                                   >  2:3006666/1‑150 (MQ=255)
                                aGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGc                                                                                                                  >  2:4478408/1‑150 (MQ=255)
                                 gggCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCa                                                                                                                 >  2:3154452/1‑150 (MQ=255)
                                 gggCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCa                                                                                                                 >  2:2828771/1‑150 (MQ=255)
                                 gggCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCa                                                                                                                 <  1:4551590/150‑1 (MQ=255)
                                  ggCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCaa                                                                                                                >  2:475089/1‑150 (MQ=255)
                                      cTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCg                                                                                                            <  2:3971586/150‑1 (MQ=255)
                                        ttATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCTGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGt                                                                                                          >  1:4020259/1‑150 (MQ=255)
                                        ttATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGt                                                                                                          >  2:420255/1‑150 (MQ=255)
                                          atatGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGTCTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaa                                                                                                        >  1:2582629/1‑150 (MQ=255)
                                          atatGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaa                                                                                                        >  1:903186/1‑150 (MQ=255)
                                              gAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATcc                                                                                                    <  2:1204646/150‑1 (MQ=255)
                                               aGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCg                                                                                                   >  2:2006295/1‑150 (MQ=255)
                                                 cGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgct                                                                                                 <  1:3655406/150‑1 (MQ=255)
                                                  ggAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctg                                                                                                >  2:850616/1‑150 (MQ=255)
                                                     aaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGctgctg                                                                                             >  2:1335674/1‑150 (MQ=255)
                                                      aaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgg                                                                                            <  1:2344572/150‑1 (MQ=255)
                                                       aCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTggg                                                                                           >  1:3256992/1‑150 (MQ=255)
                                                        ccAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgggg                                                                                          >  1:1690040/1‑150 (MQ=255)
                                                        ccAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgggg                                                                                          >  1:4074267/1‑150 (MQ=255)
                                                        ccAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgggg                                                                                          <  2:423934/150‑1 (MQ=255)
                                                          aGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGc                                                                                        >  1:3324729/1‑150 (MQ=255)
                                                            cggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGa                                                                                      >  1:1563363/1‑150 (MQ=255)
                                                              gcgcGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAg                                                                                    >  1:3154861/1‑150 (MQ=255)
                                                              gcgcGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAg                                                                                    >  2:1677230/1‑150 (MQ=255)
                                                                 cgTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTCAATCCGCTGCTGGGGGCGAAGGtg                                                                                 >  2:4307703/1‑150 (MQ=255)
                                                                 cgTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtg                                                                                 >  1:166456/1‑150 (MQ=255)
                                                                   tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTGAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgct                                                                               <  1:2928832/150‑1 (MQ=255)
                                                                   tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgct                                                                               <  1:4181017/150‑1 (MQ=255)
                                                                   tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgct                                                                               <  2:1555721/150‑1 (MQ=255)
                                                                    cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctg                                                                              >  2:3133509/1‑150 (MQ=255)
                                                                     aGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctgc                                                                             >  2:2796487/1‑150 (MQ=255)
                                                                        gagaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCg                                                                          <  2:1885990/150‑1 (MQ=255)
                                                                        gagaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCg                                                                          <  2:1112627/150‑1 (MQ=255)
                                                                         agaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTCAATCCGCTGCTGGGGGCGAAGGTGCTGCCCgg                                                                         >  1:3439318/1‑150 (MQ=255)
                                                                                aCTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACg                                                                  >  1:2469343/1‑150 (MQ=255)
                                                                                aCTCAGTATGGCGGTCCCATGGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACg                                                                  <  2:1396642/150‑1 (MQ=255)
                                                                                   cAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAc                                                               >  2:2605220/1‑150 (MQ=255)
                                                                                      tATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCtt                                                            >  2:2775144/1‑150 (MQ=255)
                                                                                      tATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCtt                                                            <  1:792600/150‑1 (MQ=255)
                                                                                        tGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTgc                                                          >  2:3779586/1‑150 (MQ=255)
                                                                                        tGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTgc                                                          >  1:4482649/1‑150 (MQ=255)
                                                                                         ggCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTgcg                                                         <  2:4154951/150‑1 (MQ=255)
                                                                                         ggCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTgcg                                                         <  1:4658134/150‑1 (MQ=255)
                                                                                            ggTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTg                                                      >  1:3645297/1‑150 (MQ=255)
                                                                                            ggTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTg                                                      >  1:2403303/1‑150 (MQ=255)
                                                                                               cccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgccc                                                   <  2:445521/150‑1 (MQ=255)
                                                                                               cccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgccc                                                   >  2:1977958/1‑150 (MQ=255)
                                                                                                ccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccg                                                  >  2:621967/1‑150 (MQ=255)
                                                                                                ccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccg                                                  >  2:776333/1‑150 (MQ=255)
                                                                                                 cATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccgg                                                 >  2:2509579/1‑150 (MQ=255)
                                                                                                 cATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccgg                                                 >  2:2681741/1‑150 (MQ=255)
                                                                                                     gTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggcccg                                             >  1:1048679/1‑150 (MQ=255)
                                                                                                     gTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggcccg                                             <  2:1646455/150‑1 (MQ=255)
                                                                                                       ccAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCt                                           >  1:2323944/1‑150 (MQ=255)
                                                                                                         aGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCAGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGc                                         >  1:1253570/1‑150 (MQ=255)
                                                                                                          gggTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGcc                                        <  1:4689975/150‑1 (MQ=255)
                                                                                                           gggtCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGGGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCg                                       <  2:833866/147‑1 (MQ=255)
                                                                                                                  cAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtc                                >  1:4395833/1‑150 (MQ=255)
                                                                                                                   aGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtct                               >  2:1266218/1‑150 (MQ=255)
                                                                                                                    ggTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctc                              <  1:1342062/150‑1 (MQ=255)
                                                                                                                      tgtAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctctc                            <  2:4843409/150‑1 (MQ=255)
                                                                                                                        tAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTccc                          >  1:4940094/1‑150 (MQ=255)
                                                                                                                             aTTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCAcc                     >  1:4025767/1‑150 (MQ=255)
                                                                                                                              ttGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCt                    >  1:4296686/1‑150 (MQ=255)
                                                                                                                              ttGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCt                    <  1:3726277/150‑1 (MQ=255)
                                                                                                                                 gcgcgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAca                 <  2:3322489/150‑1 (MQ=255)
                                                                                                                                  cgcgcCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCCTTCTCTCCCGCACCTAcag                >  2:4676898/1‑150 (MQ=255)
                                                                                                                                  cgcgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcag                >  2:4792099/1‑150 (MQ=255)
                                                                                                                                  cgcgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGGAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcag                >  2:3077458/1‑150 (MQ=255)
                                                                                                                                   gcgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcagc               >  1:2141024/1‑150 (MQ=255)
                                                                                                                                    cgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcagca              <  1:2585979/150‑1 (MQ=255)
                                                                                                                                    cgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcagca              <  2:4074267/150‑1 (MQ=255)
                                                                                                                                            ggcgtggcgtGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCgg      <  1:183854/150‑1 (MQ=255)
                                                                                                                                            ggcgtggagtGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCgg      >  2:3392993/1‑150 (MQ=255)
                                                                                                                                             gcgtggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGa     <  1:829071/150‑1 (MQ=255)
                                                                                                                                              cgtggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGAc    >  1:587269/1‑150 (MQ=255)
                                                                                                                                               gtggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACg   <  2:2200932/150‑1 (MQ=255)
                                                                                                                                               gtggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACg   <  2:2256725/150‑1 (MQ=255)
                                                                                                                                                tggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGa  >  1:4238289/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGA  >  CP009273/518673‑518966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGA  >  CP009273/518671‑518969
                                                                                                                                                     |                                                                                                                                                     
TGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGA                                                                                                                                                       <  SRR19973063.4558843/150‑1 (MQ=60)
  TTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGT                                                                                                                                                     >  SRR19973063.980487/1‑150 (MQ=60)
  TTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGT                                                                                                                                                     >  SRR19973063.980478/1‑150 (MQ=60)
  TTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGT                                                                                                                                                     >  SRR19973063.980382/1‑150 (MQ=60)
        ACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGG                                                                                                                                               >  SRR19973063.786990/1‑150 (MQ=60)
            AGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTG                                                                                                                                           >  SRR19973063.175222/1‑150 (MQ=60)
            AGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTG                                                                                                                                           >  SRR19973063.1111681/1‑150 (MQ=60)
                 TATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGG                                                                                                                                      >  SRR19973063.510056/1‑150 (MQ=60)
                 TATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGG                                                                                                                                      >  SRR19973063.509663/1‑150 (MQ=60)
                  ATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACCCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGG                                                                                                                                     >  SRR19973063.2263911/1‑150 (MQ=60)
                     AACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGG                                                                                                                                  >  SRR19973063.459111/1‑150 (MQ=60)
                               AAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCG                                                                                                                        <  SRR19973063.467350/150‑1 (MQ=60)
                                AAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGG                                                                                                                       >  SRR19973063.26966/1‑150 (MQ=60)
                                AAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGG                                                                                                                       <  SRR19973063.3891041/150‑1 (MQ=60)
                                AAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGG                                                                                                                       <  SRR19973063.3891231/150‑1 (MQ=60)
                                   GGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCA                                                                                                                    >  SRR19973063.3583443/1‑150 (MQ=60)
                                   GGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCA                                                                                                                    <  SRR19973063.4566171/150‑1 (MQ=60)
                                   GGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCA                                                                                                                    <  SRR19973063.4566115/150‑1 (MQ=60)
                                         TTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGG                                                                                                              >  SRR19973063.2454542/1‑150 (MQ=60)
                                          TTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCTGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGT                                                                                                             >  SRR19973063.4033155/1‑150 (MQ=60)
                                          TTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGT                                                                                                             >  SRR19973063.4746539/1‑150 (MQ=60)
                                           TATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTA                                                                                                            <  SRR19973063.173975/150‑1 (MQ=60)
                                           TATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCAGTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTA                                                                                                            <  SRR19973063.2245817/150‑1 (MQ=60)
                                            ATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGTCTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAA                                                                                                           >  SRR19973063.2590843/1‑150 (MQ=60)
                                            ATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAA                                                                                                           >  SRR19973063.905824/1‑150 (MQ=60)
                                             TATGAGCGGAAAACCAGCGGCGCGTCAGTGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAA                                                                                                          <  SRR19973063.16532/150‑1 (MQ=60)
                                                 AGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCG                                                                                                      >  SRR19973063.3662101/1‑150 (MQ=60)
                                                   CGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCT                                                                                                    >  SRR19973063.2358379/1‑150 (MQ=60)
                                                   CGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCT                                                                                                    <  SRR19973063.3667128/150‑1 (MQ=60)
                                                       AAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTG                                                                                                >  SRR19973063.3349263/1‑150 (MQ=60)
                                                        AACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGG                                                                                               <  SRR19973063.2351987/150‑1 (MQ=60)
                                                        AACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGG                                                                                               <  SRR19973063.2352072/150‑1 (MQ=60)
                                                         ACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGG                                                                                              >  SRR19973063.3844828/1‑150 (MQ=60)
                                                         ACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGG                                                                                              >  SRR19973063.2646295/1‑150 (MQ=60)
                                                         ACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGG                                                                                              >  SRR19973063.3267470/1‑150 (MQ=60)
                                                         ACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGG                                                                                              >  SRR19973063.3267445/1‑150 (MQ=60)
                                                         ACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGG                                                                                              >  SRR19973063.3267419/1‑150 (MQ=60)
                                                          CCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGG                                                                                             >  SRR19973063.4087321/1‑150 (MQ=60)
                                                          CCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGG                                                                                             <  SRR19973063.1412448/150‑1 (MQ=60)
                                                          CCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGG                                                                                             >  SRR19973063.4816725/1‑150 (MQ=60)
                                                          CCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGG                                                                                             >  SRR19973063.1695190/1‑150 (MQ=60)
                                                          CCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGG                                                                                             >  SRR19973063.4087547/1‑150 (MQ=60)
                                                          CCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGG                                                                                             >  SRR19973063.2481607/1‑150 (MQ=60)
                                                            AGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGC                                                                                           >  SRR19973063.704629/1‑150 (MQ=60)
                                                            AGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGC                                                                                           >  SRR19973063.3335341/1‑150 (MQ=60)
                                                              CGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGA                                                                                         >  SRR19973063.1568165/1‑150 (MQ=60)
                                                              CGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGA                                                                                         >  SRR19973063.2288414/1‑150 (MQ=60)
                                                                GCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAG                                                                                       >  SRR19973063.3165005/1‑150 (MQ=60)
                                                                   CGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTG                                                                                    >  SRR19973063.166967/1‑150 (MQ=60)
                                                                   CGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTG                                                                                    >  SRR19973063.4591812/1‑150 (MQ=60)
                                                                     TCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTGAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCT                                                                                  <  SRR19973063.2938167/150‑1 (MQ=60)
                                                                     TCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCT                                                                                  <  SRR19973063.4194369/150‑1 (MQ=60)
                                                                     TCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCT                                                                                  <  SRR19973063.1952075/150‑1 (MQ=60)
                                                                      CAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTG                                                                                 <  SRR19973063.280810/150‑1 (MQ=60)
                                                                         GGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCC                                                                              <  SRR19973063.1686910/150‑1 (MQ=60)
                                                                          GAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCG                                                                             >  SRR19973063.4071899/1‑150 (MQ=60)
                                                                          GAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCG                                                                             <  SRR19973063.867240/150‑1 (MQ=60)
                                                                          GAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCG                                                                             <  SRR19973063.827507/150‑1 (MQ=60)
                                                                           AGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTCAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGG                                                                            >  SRR19973063.3450254/1‑150 (MQ=60)
                                                                           AGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGG                                                                            <  SRR19973063.1696389/150‑1 (MQ=60)
                                                                                TGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGA                                                                       <  SRR19973063.1279668/150‑1 (MQ=60)
                                                                                  ACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACG                                                                     <  SRR19973063.3720510/150‑1 (MQ=60)
                                                                                  ACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACG                                                                     >  SRR19973063.2477234/1‑150 (MQ=60)
                                                                                      AGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACC                                                                 >  SRR19973063.1421621/1‑150 (MQ=60)
                                                                                        TATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTT                                                               <  SRR19973063.794891/150‑1 (MQ=60)
                                                                                          TGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGC                                                             >  SRR19973063.4497025/1‑150 (MQ=60)
                                                                                           GGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCTCCGGCGTGGAGTGCTCGGTGTGTCCTGGCTGGATGACTTCGGGCAACCCGGTAATTCCGCTGCTGGGGGCGAAGGTTCTGCCCGGCGAGACGGACCTTGCG                                                            <  SRR19973063.3252231/150‑1 (MQ=60)
                                                                                           GGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCG                                                            <  SRR19973063.4672999/150‑1 (MQ=60)
                                                                                           GGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCG                                                            <  SRR19973063.4673417/150‑1 (MQ=60)
                                                                                             CGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCT                                                          >  SRR19973063.414666/1‑150 (MQ=60)
                                                                                              GGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTG                                                         >  SRR19973063.3656988/1‑150 (MQ=60)
                                                                                              GGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTG                                                         >  SRR19973063.2410992/1‑150 (MQ=60)
                                                                                                 CCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCC                                                      >  SRR19973063.2365861/1‑150 (MQ=60)
                                                                                                       GTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCG                                                >  SRR19973063.1051853/1‑150 (MQ=60)
                                                                                                         CCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCT                                              >  SRR19973063.2331380/1‑150 (MQ=60)
                                                                                                           AGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCAGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGC                                            >  SRR19973063.1257303/1‑150 (MQ=60)
                                                                                                            GGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCC                                           <  SRR19973063.4704924/150‑1 (MQ=60)
                                                                                                                TCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTC                                       >  SRR19973063.2232512/1‑150 (MQ=60)
                                                                                                                  GGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCAT                                     >  SRR19973063.1758269/1‑150 (MQ=60)
                                                                                                                  GGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCAT                                     >  SRR19973063.1060393/1‑150 (MQ=60)
                                                                                                                    CAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTC                                   >  SRR19973063.4409914/1‑150 (MQ=60)
                                                                                                                    CAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTC                                   <  SRR19973063.429496/150‑1 (MQ=60)
                                                                                                                     AGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCT                                  >  SRR19973063.467716/1‑150 (MQ=60)
                                                                                                                     AGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCT                                  <  SRR19973063.4033187/150‑1 (MQ=60)
                                                                                                                      GGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTC                                 <  SRR19973063.1346089/150‑1 (MQ=60)
                                                                                                                          TAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCC                             >  SRR19973063.4956065/1‑150 (MQ=60)
                                                                                                                            AGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGC                           >  SRR19973063.3799841/1‑150 (MQ=60)
                                                                                                                            AGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGC                           >  SRR19973063.1208932/1‑150 (MQ=60)
                                                                                                                               ATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACC                        >  SRR19973063.4038677/1‑150 (MQ=60)
                                                                                                                                TTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCT                       >  SRR19973063.4310341/1‑150 (MQ=60)
                                                                                                                                TTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCT                       <  SRR19973063.3738187/150‑1 (MQ=60)
                                                                                                                                    CGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAG                   <  SRR19973063.3696166/150‑1 (MQ=60)
                                                                                                                                     GCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGC                  >  SRR19973063.2147706/1‑150 (MQ=60)
                                                                                                                                      CGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCA                 <  SRR19973063.2594201/150‑1 (MQ=60)
                                                                                                                                       GCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAG                >  SRR19973063.2974402/1‑150 (MQ=60)
                                                                                                                                        CCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGC               <  SRR19973063.421432/150‑1 (MQ=60)
                                                                                                                                        CCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGGAACTCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGC               <  SRR19973063.2671465/150‑1 (MQ=60)
                                                                                                                                          CACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTA             >  SRR19973063.2594026/1‑150 (MQ=60)
                                                                                                                                             TGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCG          >  SRR19973063.3589410/1‑150 (MQ=60)
                                                                                                                                              GGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGG         <  SRR19973063.184422/150‑1 (MQ=60)
                                                                                                                                              GGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGG         >  SRR19973063.5106479/1‑150 (MQ=60)
                                                                                                                                              GGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGG         >  SRR19973063.4336064/1‑150 (MQ=60)
                                                                                                                                              GGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGG         >  SRR19973063.4339261/1‑150 (MQ=60)
                                                                                                                                               GCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGA        <  SRR19973063.831466/150‑1 (MQ=60)
                                                                                                                                                CGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGAC       >  SRR19973063.589001/1‑150 (MQ=60)
                                                                                                                                                  TGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGTCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGA     >  SRR19973063.4419808/1‑150 (MQ=60)
                                                                                                                                                  TGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGA     >  SRR19973063.4251800/1‑150 (MQ=60)
                                                                                                                                                   GGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAA    >  SRR19973063.4079080/1‑150 (MQ=60)
                                                                                                                                                   GGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAA    <  SRR19973063.927216/150‑1 (MQ=60)
                                                                                                                                                   GGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAA    <  SRR19973063.2608384/150‑1 (MQ=60)
                                                                                                                                                     AGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGA  >  SRR19973063.615522/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGA  >  CP009273/518671‑518969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑