Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,097,943 T→A 74.8% N19Y (AAT→TAT)  csgE ← curlin secretion specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,097,9430TA74.8% 414.8 / 154.5 218N19Y (AAT→TAT) csgEcurlin secretion specificity factor
Reads supporting (aligned to +/- strand):  ref base T (24/31);  new base A (71/92);  total (95/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 218 total aligned reads displayed.

CGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCG  >  CP009273/1097795‑1098087
                                                                                                                                                    |                                                                                                                                                
  tGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATAccc                                                                                                                                               <  1:3578808/150‑1 (MQ=255)
    cACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTg                                                                                                                                             <  2:2544082/150‑1 (MQ=255)
    cACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTg                                                                                                                                             <  1:1077924/150‑1 (MQ=255)
     aCTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTgc                                                                                                                                            <  2:2897566/150‑1 (MQ=255)
      cTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTgcg                                                                                                                                           <  1:4921493/150‑1 (MQ=255)
      cTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTgcg                                                                                                                                           >  1:4932695/1‑150 (MQ=255)
      cTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTgcg                                                                                                                                           >  1:182624/1‑150 (MQ=255)
      cTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTgcg                                                                                                                                           >  2:602818/1‑150 (MQ=255)
       tGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGTCTCGGTAAAAATCAAGGACAATAGATGAAACAGTATGGTCAGTTAGCAAAACCGGGACTTCTACCTCAACGGCGTGAAGATACCCTgcgg                                                                                                                                          >  1:1980850/1‑150 (MQ=255)
       tGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTgcgg                                                                                                                                          <  1:2259796/150‑1 (MQ=255)
         ggCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTgcggcg                                                                                                                                        >  2:779673/1‑150 (MQ=255)
          gCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGa                                                                                                                                       <  1:4219502/150‑1 (MQ=255)
            cTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAAc                                                                                                                                     <  1:4087779/150‑1 (MQ=255)
                    aaTCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGGAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTc                                                                                                                             >  2:4539699/1‑150 (MQ=255)
                     aTCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCt                                                                                                                            <  2:1709699/150‑1 (MQ=255)
                       cGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTgc                                                                                                                          >  2:3649714/1‑150 (MQ=255)
                        gTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTgcc                                                                                                                         <  1:4332967/150‑1 (MQ=255)
                        gTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTgcc                                                                                                                         >  2:1120950/1‑150 (MQ=255)
                        gTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTgcc                                                                                                                         <  1:2413885/150‑1 (MQ=255)
                              ttACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCcaca                                                                                                                   <  1:189697/150‑1 (MQ=255)
                              ttACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCcaca                                                                                                                   <  2:2857929/150‑1 (MQ=255)
                               tACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACaa                                                                                                                  >  1:526199/1‑150 (MQ=255)
                                aCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAAGAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAAt                                                                                                                 <  2:2046411/150‑1 (MQ=255)
                                    gTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAg                                                                                                             <  2:1368159/150‑1 (MQ=255)
                                    gTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAg                                                                                                             <  1:3337993/150‑1 (MQ=255)
                                    gTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAg                                                                                                             >  1:1002402/1‑150 (MQ=255)
                                      ataGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCg                                                                                                           <  2:1615443/150‑1 (MQ=255)
                                      ataGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCg                                                                                                           <  2:638312/150‑1 (MQ=255)
                                               ttCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACg                                                                                                  >  2:4860865/1‑150 (MQ=255)
                                                tCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGt                                                                                                 >  1:1624791/1‑150 (MQ=255)
                                                    aTTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCa                                                                                             <  2:4058630/150‑1 (MQ=255)
                                                      ttATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATg                                                                                           >  2:1532655/1‑150 (MQ=255)
                                                      ttATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATg                                                                                           <  2:1030673/150‑1 (MQ=255)
                                                       tATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATgg                                                                                          <  2:461474/150‑1 (MQ=255)
                                                         tCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCt                                                                                        <  2:72262/150‑1 (MQ=255)
                                                          cACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCtt                                                                                       <  2:562780/150‑1 (MQ=255)
                                                           aCTAAAGGCTCGGTAAGAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCttt                                                                                      >  2:4174742/1‑150 (MQ=255)
                                                            cTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTa                                                                                     >  2:1874024/1‑150 (MQ=255)
                                                             tAAAGGCTCGTTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTAt                                                                                    <  2:876926/150‑1 (MQ=255)
                                                             tAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTAt                                                                                    <  1:2208125/150‑1 (MQ=255)
                                                              aaaGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATc                                                                                   <  2:1795382/150‑1 (MQ=255)
                                                              aaaGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATc                                                                                   >  1:4039206/1‑150 (MQ=255)
                                                              aaaGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATc                                                                                   <  2:3059360/150‑1 (MQ=255)
                                                               aaGGCTCGGTAAAAATCATGGACAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCg                                                                                  >  2:4908715/1‑150 (MQ=255)
                                                                    tCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGa                                                                             <  2:2392917/150‑1 (MQ=255)
                                                                      ggTAAAAATCATGGCCAATAGCTGAAACAGTATGGTCAGTTAGCCATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAAGTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAgg                                                                           <  2:4707114/150‑1 (MQ=255)
                                                                      ggTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAgg                                                                           <  2:4347799/150‑1 (MQ=255)
                                                                      ggTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAAGAACGTTTCATGGCTTTATCGCCTGAgg                                                                           <  1:1494754/150‑1 (MQ=255)
                                                                       gTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGt                                                                          <  1:1424149/150‑1 (MQ=255)
                                                                       gTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGt                                                                          <  2:4982549/150‑1 (MQ=255)
                                                                        tAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGtt                                                                         <  2:3563938/150‑1 (MQ=255)
                                                                           aaaTCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATc                                                                      <  2:2137671/150‑1 (MQ=255)
                                                                           aaaTCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATc                                                                      >  1:3847051/1‑150 (MQ=255)
                                                                            aaTCATGGCCAATAGATGAATCAGTATGGTCAGTTAGCTATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCg                                                                     <  2:2654029/150‑1 (MQ=255)
                                                                              tCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGtt                                                                   >  1:2553213/1‑150 (MQ=255)
                                                                                aTGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTg                                                                 >  2:1224798/1‑150 (MQ=255)
                                                                                aTGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTg                                                                 >  2:3159898/1‑150 (MQ=255)
                                                                                    ccAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCa                                                             <  1:1310602/150‑1 (MQ=255)
                                                                                     cAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAg                                                            >  1:1045001/1‑150 (MQ=255)
                                                                                      aaTAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAgg                                                           <  1:1767992/150‑1 (MQ=255)
                                                                                      aaTAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAgg                                                           >  1:1875077/1‑150 (MQ=255)
                                                                                       aTAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGa                                                          <  1:4436482/150‑1 (MQ=255)
                                                                                        tAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGaa                                                         >  1:3939847/1‑150 (MQ=255)
                                                                                           aTGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAAcc                                                      <  1:1616052/150‑1 (MQ=255)
                                                                                               aaCAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCtt                                                  >  1:920518/1‑150 (MQ=255)
                                                                                                aCAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAGACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTtg                                                 <  1:4797503/150‑1 (MQ=255)
                                                                                                  aGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTtgtg                                               >  2:4292781/1‑150 (MQ=255)
                                                                                                  aGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTtgtg                                               <  1:3370593/150‑1 (MQ=255)
                                                                                                  aGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTtgtg                                               <  2:3674008/150‑1 (MQ=255)
                                                                                                     aTGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTcc                                            >  2:1435965/1‑150 (MQ=255)
                                                                                                      tGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCg                                           <  2:535990/150‑1 (MQ=255)
                                                                                                       ggTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCgg                                          >  1:5009841/1‑150 (MQ=255)
                                                                                                       ggTCAGTTAGCAAGCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCgg                                          <  2:1268161/150‑1 (MQ=255)
                                                                                                         tCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGtt                                        <  1:258426/150‑1 (MQ=255)
                                                                                                          cAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGttt                                       >  2:3049069/1‑150 (MQ=255)
                                                                                                                gCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACg                                 >  2:1526786/1‑150 (MQ=255)
                                                                                                                 cAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACgg                                >  1:3954544/1‑150 (MQ=255)
                                                                                                                   aTCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCt                              <  1:1889618/150‑1 (MQ=255)
                                                                                                                    tCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTa                             <  1:4832342/150‑1 (MQ=255)
                                                                                                                     cccGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAt                            >  1:1055524/1‑150 (MQ=255)
                                                                                                                      ccGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAtc                           >  1:2096110/1‑150 (MQ=255)
                                                                                                                      ccGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAtc                           <  1:4761850/150‑1 (MQ=255)
                                                                                                                       cGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAtct                          <  1:4243067/150‑1 (MQ=255)
                                                                                                                        gggACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAtctt                         >  1:3609223/1‑150 (MQ=255)
                                                                                                                          gACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAtcttct                       <  1:4600186/150‑1 (MQ=255)
                                                                                                                          gACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAtcttct                       <  2:3352914/150‑1 (MQ=255)
                                                                                                                           aCTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATcttctt                      >  2:3443130/1‑150 (MQ=255)
                                                                                                                           aCTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATcttctt                      <  2:4932695/150‑1 (MQ=255)
                                                                                                                            cTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTg                     >  2:4302347/1‑150 (MQ=255)
                                                                                                                               cTACCTCTCCGGCGTGAAGAGACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGaaa                  <  1:4265323/150‑1 (MQ=255)
                                                                                                                               cTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGaaa                  <  1:1687628/150‑1 (MQ=255)
                                                                                                                                    tCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGAtt             >  2:4768258/1‑150 (MQ=255)
                                                                                                                                     cAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATta            <  1:1273124/150‑1 (MQ=255)
                                                                                                                                       aCGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATtata          >  2:1367038/1‑150 (MQ=255)
                                                                                                                                       aCGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACCTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATtata          >  2:1367195/1‑150 (MQ=255)
                                                                                                                                           cGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGa      >  1:4912319/1‑150 (MQ=255)
                                                                                                                                            gTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGAt     >  2:4056871/1‑150 (MQ=255)
                                                                                                                                            gTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGAt     >  2:3791471/1‑150 (MQ=255)
                                                                                                                                             tGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATg    <  1:81459/150‑1 (MQ=255)
                                                                                                                                               aaGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCCGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCg  <  2:4562438/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCG  >  CP009273/1097795‑1098087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCGTTTT  >  CP009273/1097794‑1098091
                                                                                                                                                     |                                                                                                                                                    
TCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATA                                                                                                                                                      <  SRR19973063.2673194/150‑1 (MQ=60)
 CGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATAC                                                                                                                                                     >  SRR19973063.1950628/1‑150 (MQ=60)
   TGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCC                                                                                                                                                   <  SRR19973063.3590281/150‑1 (MQ=60)
     CACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTG                                                                                                                                                 <  SRR19973063.1081200/150‑1 (MQ=60)
     CACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTG                                                                                                                                                 >  SRR19973063.5149088/1‑150 (MQ=60)
       CTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCG                                                                                                                                               >  SRR19973063.183272/1‑150 (MQ=60)
       CTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCG                                                                                                                                               >  SRR19973063.183189/1‑150 (MQ=60)
       CTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCG                                                                                                                                               <  SRR19973063.4937497/150‑1 (MQ=60)
       CTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCG                                                                                                                                               >  SRR19973063.4948630/1‑150 (MQ=60)
       CTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCG                                                                                                                                               <  SRR19973063.4937387/150‑1 (MQ=60)
        TGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGTCTCGGTAAAAATCAAGGACAATAGATGAAACAGTATGGTCAGTTAGCAAAACCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGG                                                                                                                                              >  SRR19973063.1987044/1‑150 (MQ=60)
        TGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGG                                                                                                                                              <  SRR19973063.2266989/150‑1 (MQ=60)
           GCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGA                                                                                                                                           <  SRR19973063.4232954/150‑1 (MQ=60)
             CTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAAC                                                                                                                                         <  SRR19973063.4100876/150‑1 (MQ=60)
                   TTAATCGTTAAGTTACCCGTATAGGCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAAT                                                                                                                                   >  SRR19973063.3863196/1‑150 (MQ=60)
                     AATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTC                                                                                                                                 <  SRR19973063.4067648/150‑1 (MQ=60)
                         GTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCC                                                                                                                             <  SRR19973063.4346772/150‑1 (MQ=60)
                         GTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCC                                                                                                                             <  SRR19973063.2421607/150‑1 (MQ=60)
                         GTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCC                                                                                                                             <  SRR19973063.1621036/150‑1 (MQ=60)
                          TTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCG                                                                                                                            >  SRR19973063.1580592/1‑150 (MQ=60)
                             AGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCA                                                                                                                         >  SRR19973063.128057/1‑150 (MQ=60)
                               TTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACA                                                                                                                       <  SRR19973063.190279/150‑1 (MQ=60)
                                TACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAA                                                                                                                      >  SRR19973063.527739/1‑150 (MQ=60)
                                 ACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAAT                                                                                                                     <  SRR19973063.4696772/150‑1 (MQ=60)
                                 ACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAAT                                                                                                                     <  SRR19973063.483045/150‑1 (MQ=60)
                                 ACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAAT                                                                                                                     <  SRR19973063.628342/150‑1 (MQ=60)
                                     GTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAG                                                                                                                 <  SRR19973063.3348635/150‑1 (MQ=60)
                                     GTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAG                                                                                                                 >  SRR19973063.1005424/1‑150 (MQ=60)
                                     GTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAG                                                                                                                 <  SRR19973063.1384576/150‑1 (MQ=60)
                                       ATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCG                                                                                                               <  SRR19973063.304617/150‑1 (MQ=60)
                                            CACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAAT                                                                                                          >  SRR19973063.1715861/1‑150 (MQ=60)
                                                TTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACG                                                                                                      >  SRR19973063.3569253/1‑150 (MQ=60)
                                                 TCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGT                                                                                                     >  SRR19973063.1629755/1‑150 (MQ=60)
                                                    CATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTC                                                                                                  >  SRR19973063.1218800/1‑150 (MQ=60)
                                                     ATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCA                                                                                                 <  SRR19973063.2381149/150‑1 (MQ=60)
                                                      TTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCAT                                                                                                <  SRR19973063.4340913/150‑1 (MQ=60)
                                                          TCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCT                                                                                            <  SRR19973063.4798467/150‑1 (MQ=60)
                                                              TAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTAT                                                                                        <  SRR19973063.2215081/150‑1 (MQ=60)
                                                              TAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTAT                                                                                        >  SRR19973063.1357805/1‑150 (MQ=60)
                                                              TAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTAT                                                                                        <  SRR19973063.2215121/150‑1 (MQ=60)
                                                              TAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTAT                                                                                        <  SRR19973063.2214998/150‑1 (MQ=60)
                                                               AAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATC                                                                                       >  SRR19973063.4052154/1‑150 (MQ=60)
                                                                 AGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGC                                                                                     >  SRR19973063.1049331/1‑150 (MQ=60)
                                                                  GGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCC                                                                                    <  SRR19973063.2022880/150‑1 (MQ=60)
                                                                      CGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAG                                                                                <  SRR19973063.1669791/150‑1 (MQ=60)
                                                                       GGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGG                                                                               <  SRR19973063.1499245/150‑1 (MQ=60)
                                                                       GGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAAGAACGTTTCATGGCTTTATCGCCTGAGG                                                                               <  SRR19973063.1499333/150‑1 (MQ=60)
                                                                        GTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGT                                                                              <  SRR19973063.1428482/150‑1 (MQ=60)
                                                                        GTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGT                                                                              <  SRR19973063.4015077/150‑1 (MQ=60)
                                                                           AAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTAT                                                                           <  SRR19973063.4191903/150‑1 (MQ=60)
                                                                            AAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATC                                                                          >  SRR19973063.3859285/1‑150 (MQ=60)
                                                                            AAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCACAAAGGCGTGAAGATACCCTGCGGCGAACAGAAATTATGACGCCACAATCCAGCGTAAATAACGTATCATGGCTATATCGCCTGAGGTTATC                                                                          >  SRR19973063.3859338/1‑150 (MQ=60)
                                                                             AATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCG                                                                         <  SRR19973063.2576360/150‑1 (MQ=60)
                                                                               TCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTT                                                                       >  SRR19973063.907822/1‑150 (MQ=60)
                                                                               TCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTT                                                                       >  SRR19973063.2561348/1‑150 (MQ=60)
                                                                                     CCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCA                                                                 <  SRR19973063.1314509/150‑1 (MQ=60)
                                                                                     CCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCA                                                                 <  SRR19973063.4241932/150‑1 (MQ=60)
                                                                                      CAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAG                                                                >  SRR19973063.1048166/1‑150 (MQ=60)
                                                                                       AATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGG                                                               <  SRR19973063.1773438/150‑1 (MQ=60)
                                                                                       AATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGG                                                               >  SRR19973063.1880937/1‑150 (MQ=60)
                                                                                        ATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGA                                                              <  SRR19973063.4450723/150‑1 (MQ=60)
                                                                                         TAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAA                                                             >  SRR19973063.3952443/1‑150 (MQ=60)
                                                                                            ATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACC                                                          <  SRR19973063.3366271/150‑1 (MQ=60)
                                                                                            ATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACC                                                          <  SRR19973063.1620996/150‑1 (MQ=60)
                                                                                                AACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTT                                                      >  SRR19973063.923236/1‑150 (MQ=60)
                                                                                                 ACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAGACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTG                                                     <  SRR19973063.4812838/150‑1 (MQ=60)
                                                                                                  CAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGT                                                    >  SRR19973063.1887273/1‑150 (MQ=60)
                                                                                                   AGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTG                                                   <  SRR19973063.3958815/150‑1 (MQ=60)
                                                                                                   AGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTG                                                   <  SRR19973063.3381325/150‑1 (MQ=60)
                                                                                                    GTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGT                                                  <  SRR19973063.1830763/150‑1 (MQ=60)
                                                                                                    GTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGT                                                  <  SRR19973063.1830814/150‑1 (MQ=60)
                                                                                                      ATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCC                                                <  SRR19973063.3092734/150‑1 (MQ=60)
                                                                                                        GGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGG                                              >  SRR19973063.5026012/1‑150 (MQ=60)
                                                                                                          TCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTT                                            <  SRR19973063.259199/150‑1 (MQ=60)
                                                                                                          TCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTT                                            >  SRR19973063.490348/1‑150 (MQ=60)
                                                                                                            AGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTT                                          <  SRR19973063.4507568/150‑1 (MQ=60)
                                                                                                              TTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTT                                        <  SRR19973063.4261016/150‑1 (MQ=60)
                                                                                                              TTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTT                                        <  SRR19973063.4261029/150‑1 (MQ=60)
                                                                                                                  CAATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGG                                    >  SRR19973063.3967196/1‑150 (MQ=60)
                                                                                                                    ATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCT                                  <  SRR19973063.4933318/150‑1 (MQ=60)
                                                                                                                    ATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCT                                  <  SRR19973063.1895529/150‑1 (MQ=60)
                                                                                                                    ATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCT                                  <  SRR19973063.1918143/150‑1 (MQ=60)
                                                                                                                    ATCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCT                                  <  SRR19973063.1895742/150‑1 (MQ=60)
                                                                                                                     TCCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTA                                 <  SRR19973063.4847844/150‑1 (MQ=60)
                                                                                                                      CCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAT                                >  SRR19973063.1058718/1‑150 (MQ=60)
                                                                                                                      CCCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTAT                                >  SRR19973063.4791482/1‑150 (MQ=60)
                                                                                                                       CCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATC                               >  SRR19973063.2102658/1‑150 (MQ=60)
                                                                                                                       CCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTGGTGTCCGGTTTTTTACGGCTATC                               >  SRR19973063.2102462/1‑150 (MQ=60)
                                                                                                                       CCGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATC                               <  SRR19973063.4777032/150‑1 (MQ=60)
                                                                                                                        CGGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCT                              <  SRR19973063.4256592/150‑1 (MQ=60)
                                                                                                                         GGGACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTT                             >  SRR19973063.3620810/1‑150 (MQ=60)
                                                                                                                           GACTTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCT                           <  SRR19973063.4614906/150‑1 (MQ=60)
                                                                                                                            ACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTT                          <  SRR19973063.1023215/150‑1 (MQ=60)
                                                                                                                            ACGTCTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTT                          <  SRR19973063.3607520/150‑1 (MQ=60)
                                                                                                                                CTACCTCTCCGGCGTGAAGAGACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAA                      <  SRR19973063.4278889/150‑1 (MQ=60)
                                                                                                                                CTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAA                      >  SRR19973063.4438875/1‑150 (MQ=60)
                                                                                                                                CTACCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAA                      <  SRR19973063.1692770/150‑1 (MQ=60)
                                                                                                                                   CCTCAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGA                   >  SRR19973063.1071545/1‑150 (MQ=60)
                                                                                                                                      CAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTA                >  SRR19973063.4977098/1‑150 (MQ=60)
                                                                                                                                      CAACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTA                <  SRR19973063.1276912/150‑1 (MQ=60)
                                                                                                                                       AACGGCGTGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTAT               <  SRR19973063.5051876/150‑1 (MQ=60)
                                                                                                                                          GGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAA            <  SRR19973063.3697740/150‑1 (MQ=60)
                                                                                                                                            CGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGA          >  SRR19973063.4928184/1‑150 (MQ=60)
                                                                                                                                              TGAAGATACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATG        <  SRR19973063.81742/150‑1 (MQ=60)
                                                                                                                                                AAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCG      >  SRR19973063.2530846/1‑150 (MQ=60)
                                                                                                                                                    TACCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCGTTTT  >  SRR19973063.3381573/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCGTTTT  >  CP009273/1097794‑1098091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑