Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,122,994 C→A 100% R245S (CGC→AGC)  yceM → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,122,9940CA97.4% 854.0 / 10.8 267R245S (CGC→AGC) yceMputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (6/1);  new base A (115/145);  total (121/146)
Fisher's exact test for biased strand distribution p-value = 4.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 270 total aligned reads displayed.

CTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCG  >  CP009273/1122847‑1123140
                                                                                                                                                   |                                                                                                                                                  
cTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAgagc                                                                                                                                                  >  1:1153541/1‑150 (MQ=255)
   tttGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCggg                                                                                                                                               <  1:2387123/150‑1 (MQ=255)
      gCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGCATGGCGTGAGGAGAGCGGGCAg                                                                                                                                            <  2:4861740/150‑1 (MQ=255)
        cGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAggg                                                                                                                                          <  1:875854/150‑1 (MQ=255)
          aGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATCTGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCg                                                                                                                                        >  1:3129174/1‑150 (MQ=255)
          aGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCg                                                                                                                                        <  2:3342795/150‑1 (MQ=255)
          aGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCg                                                                                                                                        <  2:3765794/150‑1 (MQ=255)
            cACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTa                                                                                                                                      >  2:1772503/1‑150 (MQ=255)
                aTTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGc                                                                                                                                  >  2:1531218/1‑150 (MQ=255)
                aTTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGc                                                                                                                                  >  2:4416989/1‑150 (MQ=255)
                 tttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCa                                                                                                                                 <  1:785039/150‑1 (MQ=255)
                    ttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATaa                                                                                                                              >  1:3048944/1‑150 (MQ=255)
                    ttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATaa                                                                                                                              <  2:3025432/150‑1 (MQ=255)
                      cGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAc                                                                                                                            <  2:5055174/150‑1 (MQ=255)
                      cGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAc                                                                                                                            >  1:2982256/1‑150 (MQ=255)
                       ggCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAcc                                                                                                                           <  2:201267/150‑1 (MQ=255)
                        gCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCg                                                                                                                          >  1:3850907/1‑150 (MQ=255)
                           ggTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGAtt                                                                                                                       >  1:1470710/1‑150 (MQ=255)
                             tCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTcc                                                                                                                     <  2:2294662/150‑1 (MQ=255)
                               cTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTg                                                                                                                   <  1:4766710/150‑1 (MQ=255)
                                 ttGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGt                                                                                                                 >  1:1296025/1‑150 (MQ=255)
                                  tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCCGCGTGAAACCGTGCAGGCCGTGACGGACGGTGCGCTCATCGACCTTACGGATATGCGCGAATGGCGGGAGGAGCGCGGGCAGGGCGTTGTGCATACACCGATTCCTGGtt                                                                                                                >  2:4667898/1‑150 (MQ=255)
                                  tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGtt                                                                                                                >  1:948168/1‑150 (MQ=255)
                                  tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGtt                                                                                                                >  1:4069543/1‑150 (MQ=255)
                                    cAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTgg                                                                                                              <  1:4033369/150‑1 (MQ=255)
                                    cAGATCACCACCTGAAAGCATCGCCGTGCCGGAAGTCACCGTGAAACCGTGCACGCCGAGACAGACGGTGCGCACATCGACATTAAGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTgg                                                                                                              >  2:1971658/1‑150 (MQ=255)
                                      gATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCa                                                                                                            >  2:3552455/1‑150 (MQ=255)
                                           ccaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTa                                                                                                       <  2:3442196/150‑1 (MQ=255)
                                            caccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAc                                                                                                      <  1:1928089/150‑1 (MQ=255)
                                            caccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAc                                                                                                      >  1:4513349/1‑150 (MQ=255)
                                             accTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACAGTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACg                                                                                                     >  2:4393917/1‑150 (MQ=255)
                                              ccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGc                                                                                                    >  2:2154646/1‑150 (MQ=255)
                                               cTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCt                                                                                                   >  1:4200303/1‑150 (MQ=255)
                                               cTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCt                                                                                                   >  1:3524529/1‑150 (MQ=255)
                                               cTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCt                                                                                                   <  1:253679/150‑1 (MQ=255)
                                                tGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCtt                                                                                                  >  2:1840356/1‑150 (MQ=255)
                                                  taggCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGa                                                                                                <  2:3948100/147‑1 (MQ=255)
                                                     gCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCa                                                                                             >  2:825547/1‑150 (MQ=255)
                                                       aTCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACGGACGGGGCGCTCATCGACAGTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAACCCGATTCCTGGTTGGCAGAGTACGCTTGAGCAAc                                                                                           <  2:2479726/150‑1 (MQ=255)
                                                          gCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTg                                                                                        <  2:2147473/150‑1 (MQ=255)
                                                           ccGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTgg                                                                                       <  2:1504443/150‑1 (MQ=255)
                                                              tGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGtt                                                                                    <  1:4913340/150‑1 (MQ=255)
                                                                ccGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTg                                                                                  <  1:2612275/150‑1 (MQ=255)
                                                                ccGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTg                                                                                  <  2:3499296/150‑1 (MQ=255)
                                                                 cGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGt                                                                                 >  2:4816237/1‑150 (MQ=255)
                                                                  ggAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTc                                                                                >  1:2505036/1‑150 (MQ=255)
                                                                    aaGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCgg                                                                              >  1:517629/1‑150 (MQ=255)
                                                                      gTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCt                                                                            <  1:4333210/150‑1 (MQ=255)
                                                                        cAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtgt                                                                          <  2:402901/150‑1 (MQ=255)
                                                                            gTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTgcgc                                                                      >  1:3931031/1‑150 (MQ=255)
                                                                             tGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGcgcg                                                                     <  1:2073819/150‑1 (MQ=255)
                                                                              gAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCgg                                                                    <  2:4445627/150‑1 (MQ=255)
                                                                              gAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCgg                                                                    <  2:594574/150‑1 (MQ=255)
                                                                               aaaCCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGc                                                                   >  1:5020737/1‑150 (MQ=255)
                                                                                aaCCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCa                                                                  >  1:1352509/1‑150 (MQ=255)
                                                                                    gTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTc                                                              <  2:2771319/150‑1 (MQ=255)
                                                                                     tGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCa                                                             <  2:4942889/150‑1 (MQ=255)
                                                                                      gCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAt                                                            <  1:2825093/150‑1 (MQ=255)
                                                                                           ccGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAt                                                       <  2:2235086/150‑1 (MQ=255)
                                                                                            cGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtg                                                      >  2:2531579/1‑150 (MQ=255)
                                                                                              tgactgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAtt                                                           >  2:2952835/1‑143 (MQ=255)
                                                                                              tgactgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtg                                                    >  1:3322116/1‑150 (MQ=255)
                                                                                               gactgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtgt                                                   <  2:3482336/150‑1 (MQ=255)
                                                                                                 ctgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGc                                                 <  2:471633/150‑1 (MQ=255)
                                                                                                 ctgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGc                                                 <  1:4867213/150‑1 (MQ=255)
                                                                                                  tgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCTCGTCACTTCATTGAATGTGTGCa                                                >  1:1307377/1‑150 (MQ=255)
                                                                                                       gTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAAcc                                           <  1:5087892/150‑1 (MQ=255)
                                                                                                       gTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTACATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAAcc                                           <  2:481071/150‑1 (MQ=255)
                                                                                                         gcgcTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAg                                         <  2:3796938/150‑1 (MQ=255)
                                                                                                          cgcTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGa                                        <  1:4806119/150‑1 (MQ=255)
                                                                                                            cTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACa                                      >  1:2940004/1‑150 (MQ=255)
                                                                                                             tCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAg                                     <  1:1992213/150‑1 (MQ=255)
                                                                                                              cATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAATCCAGACAGt                                    <  1:825547/150‑1 (MQ=255)
                                                                                                              cATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGt                                    >  2:4956970/1‑150 (MQ=255)
                                                                                                                   aCATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGc                               >  2:2648872/1‑150 (MQ=255)
                                                                                                                   aCATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGc                               <  1:3789063/150‑1 (MQ=255)
                                                                                                                    taTTACGGATATGCGCGAATTGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCa                              >  1:1874485/2‑150 (MQ=255)
                                                                                                                      ttACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCaaa                            <  1:1078209/150‑1 (MQ=255)
                                                                                                                       tACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAc                           <  1:2185083/150‑1 (MQ=255)
                                                                                                                        aCGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAcc                          <  2:2581771/150‑1 (MQ=255)
                                                                                                                          ggATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgc                        <  1:2099385/150‑1 (MQ=255)
                                                                                                                           gATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgcc                       >  2:984711/1‑150 (MQ=255)
                                                                                                                            atatGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgccg                      <  2:649929/150‑1 (MQ=255)
                                                                                                                            atatGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgccg                      >  2:3731200/1‑150 (MQ=255)
                                                                                                                             tatGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCgg                     >  1:969761/1‑150 (MQ=255)
                                                                                                                             tatGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCgg                     >  1:3549916/1‑150 (MQ=255)
                                                                                                                              atGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGc                    <  2:406252/150‑1 (MQ=255)
                                                                                                                                  gcgAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAAc                <  2:3229346/150‑1 (MQ=255)
                                                                                                                                   cgAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACa               <  2:1296184/150‑1 (MQ=255)
                                                                                                                                   cgAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACa               <  1:3294199/150‑1 (MQ=255)
                                                                                                                                     aaTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCATCGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAgg             <  2:3666588/150‑1 (MQ=255)
                                                                                                                                       tGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGcc           <  1:2438255/150‑1 (MQ=255)
                                                                                                                                       tGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGcc           >  2:1665872/1‑150 (MQ=255)
                                                                                                                                        ggCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCg          >  2:1082102/1‑150 (MQ=255)
                                                                                                                                        ggCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCg          >  2:2065283/1‑150 (MQ=255)
                                                                                                                                         gCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGt         <  2:3048944/150‑1 (MQ=255)
                                                                                                                                           gTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATAGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGc       >  1:2642281/1‑150 (MQ=255)
                                                                                                                                            tGAGGAGAGCGGGCAGGGCGTAGTGCGGAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCt      >  2:1747230/1‑150 (MQ=255)
                                                                                                                                               ggagAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGgc   >  2:1977493/1‑150 (MQ=255)
                                                                                                                                                gagCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGgcg  >  1:4430373/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCG  >  CP009273/1122847‑1123140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAA  >  CP009273/1122846‑1123143
                                                                                                                                                    |                                                                                                                                                     
GCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAG                                                                                                                                                      >  SRR19973063.3099103/1‑150 (MQ=60)
 CTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGC                                                                                                                                                     >  SRR19973063.1157021/1‑150 (MQ=60)
 CTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGC                                                                                                                                                     >  SRR19973063.2985876/1‑150 (MQ=60)
   GTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGG                                                                                                                                                   >  SRR19973063.4898084/1‑150 (MQ=60)
    TTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGG                                                                                                                                                  >  SRR19973063.912002/1‑150 (MQ=60)
    TTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGG                                                                                                                                                  <  SRR19973063.2394766/150‑1 (MQ=60)
      TGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCA                                                                                                                                                <  SRR19973063.840821/150‑1 (MQ=60)
      TGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCA                                                                                                                                                <  SRR19973063.840810/150‑1 (MQ=60)
       GCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAG                                                                                                                                               <  SRR19973063.952608/150‑1 (MQ=60)
        CCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGG                                                                                                                                              <  SRR19973063.4820196/150‑1 (MQ=60)
         CGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGG                                                                                                                                             >  SRR19973063.4182496/1‑150 (MQ=60)
         CGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGG                                                                                                                                             >  SRR19973063.2423616/1‑150 (MQ=60)
         CGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGG                                                                                                                                             >  SRR19973063.2423614/1‑150 (MQ=60)
         CGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGG                                                                                                                                             <  SRR19973063.878376/150‑1 (MQ=60)
           AGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATCTGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCG                                                                                                                                           >  SRR19973063.3139246/1‑150 (MQ=60)
           AGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCG                                                                                                                                           >  SRR19973063.3139206/1‑150 (MQ=60)
              ACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAG                                                                                                                                        >  SRR19973063.3860263/1‑150 (MQ=60)
              ACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAG                                                                                                                                        <  SRR19973063.4303483/150‑1 (MQ=60)
                CATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTA                                                                                                                                      <  SRR19973063.1400941/150‑1 (MQ=60)
                  TTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCA                                                                                                                                    <  SRR19973063.787313/150‑1 (MQ=60)
                   TTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCAT                                                                                                                                   <  SRR19973063.5176481/150‑1 (MQ=60)
                     ctccGCTGGTCCTTCGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAA                                                                                                                                 <  SRR19973063.2680474/146‑1 (MQ=60)
                     TTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAA                                                                                                                                 >  SRR19973063.3058702/1‑150 (MQ=60)
                       CGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAC                                                                                                                               >  SRR19973063.2991781/1‑150 (MQ=60)
                        GGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACC                                                                                                                              >  SRR19973063.260485/1‑150 (MQ=60)
                         GCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCG                                                                                                                             >  SRR19973063.3863153/1‑150 (MQ=60)
                         GCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCG                                                                                                                             >  SRR19973063.3863081/1‑150 (MQ=60)
                            GGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATT                                                                                                                          >  SRR19973063.1475207/1‑150 (MQ=60)
                            GGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATT                                                                                                                          >  SRR19973063.4734964/1‑150 (MQ=60)
                            GGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATT                                                                                                                          >  SRR19973063.2739185/1‑150 (MQ=60)
                               CCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCT                                                                                                                       <  SRR19973063.2086720/150‑1 (MQ=60)
                               CCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCT                                                                                                                       >  SRR19973063.426798/1‑150 (MQ=60)
                                CTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTG                                                                                                                      <  SRR19973063.4781918/150‑1 (MQ=60)
                                  TTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGT                                                                                                                    >  SRR19973063.1299881/1‑150 (MQ=60)
                                  TTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGT                                                                                                                    <  SRR19973063.815247/150‑1 (MQ=60)
                                   TGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTT                                                                                                                   >  SRR19973063.3539620/1‑150 (MQ=60)
                                   TGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTT                                                                                                                   >  SRR19973063.951006/1‑150 (MQ=60)
                                   TGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTT                                                                                                                   >  SRR19973063.4082584/1‑150 (MQ=60)
                                     CAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGG                                                                                                                 <  SRR19973063.4046295/150‑1 (MQ=60)
                                      AGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGC                                                                                                                >  SRR19973063.4398664/1‑150 (MQ=60)
                                        ATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAG                                                                                                              <  SRR19973063.491291/150‑1 (MQ=60)
                                          CACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAG                                                                                                            <  SRR19973063.3054938/150‑1 (MQ=60)
                                             CACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAC                                                                                                         >  SRR19973063.3996654/1‑150 (MQ=60)
                                             CACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAC                                                                                                         <  SRR19973063.1934123/150‑1 (MQ=60)
                                             CACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAC                                                                                                         >  SRR19973063.4527840/1‑150 (MQ=60)
                                             CACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAC                                                                                                         <  SRR19973063.1934115/150‑1 (MQ=60)
                                             CACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAC                                                                                                         >  SRR19973063.1423729/1‑150 (MQ=60)
                                                CTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCT                                                                                                      <  SRR19973063.254444/150‑1 (MQ=60)
                                                CTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCT                                                                                                      >  SRR19973063.4213707/1‑150 (MQ=60)
                                                CTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCT                                                                                                      >  SRR19973063.3535811/1‑150 (MQ=60)
                                                CTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCT                                                                                                      >  SRR19973063.3531908/1‑150 (MQ=60)
                                                   TATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGA                                                                                                   >  SRR19973063.3164435/1‑150 (MQ=60)
                                                     TGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGC                                                                                                 >  SRR19973063.3176695/1‑150 (MQ=60)
                                                      GCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCA                                                                                                <  SRR19973063.2082605/150‑1 (MQ=60)
                                                        ATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAAC                                                                                              >  SRR19973063.4972454/1‑150 (MQ=60)
                                                        ATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAAC                                                                                              >  SRR19973063.1738665/1‑150 (MQ=60)
                                                         TCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACG                                                                                             >  SRR19973063.1881132/1‑150 (MQ=60)
                                                             CGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGG                                                                                         >  SRR19973063.2028659/1‑150 (MQ=60)
                                                               TGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTT                                                                                       <  SRR19973063.4929255/150‑1 (MQ=60)
                                                               TGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTT                                                                                       >  SRR19973063.1139686/1‑150 (MQ=60)
                                                               TGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTT                                                                                       <  SRR19973063.4929207/150‑1 (MQ=60)
                                                                GCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCTGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTT                                                                                      <  SRR19973063.1313913/150‑1 (MQ=60)
                                                                 CCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTG                                                                                     <  SRR19973063.2620585/150‑1 (MQ=60)
                                                                 CCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTG                                                                                     >  SRR19973063.3926800/1‑150 (MQ=60)
                                                                 CCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTG                                                                                     <  SRR19973063.2620470/150‑1 (MQ=60)
                                                                  CGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGT                                                                                    >  SRR19973063.4830727/1‑150 (MQ=60)
                                                                  CGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGT                                                                                    >  SRR19973063.4830720/1‑150 (MQ=60)
                                                                   GGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTC                                                                                   >  SRR19973063.2513041/1‑150 (MQ=60)
                                                                   GGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTC                                                                                   <  SRR19973063.4514257/150‑1 (MQ=60)
                                                                   GGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTC                                                                                   >  SRR19973063.2513024/1‑150 (MQ=60)
                                                                    GAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCG                                                                                  <  SRR19973063.1309236/150‑1 (MQ=60)
                                                                     AAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGG                                                                                 >  SRR19973063.519140/1‑150 (MQ=60)
                                                                       GTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCT                                                                               <  SRR19973063.1263004/150‑1 (MQ=60)
                                                                       GTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCT                                                                               <  SRR19973063.4347015/150‑1 (MQ=60)
                                                                             GTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGC                                                                         >  SRR19973063.3943583/1‑150 (MQ=60)
                                                                              TGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCG                                                                        <  SRR19973063.2080304/150‑1 (MQ=60)
                                                                              TGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCG                                                                        <  SRR19973063.932671/150‑1 (MQ=60)
                                                                              TGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCG                                                                        <  SRR19973063.2080299/150‑1 (MQ=60)
                                                                                AAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGC                                                                      >  SRR19973063.5036937/1‑150 (MQ=60)
                                                                                 AACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCA                                                                     >  SRR19973063.1356570/1‑150 (MQ=60)
                                                                                     GTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTC                                                                 <  SRR19973063.3128737/150‑1 (MQ=60)
                                                                                     GTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTC                                                                 >  SRR19973063.407397/1‑150 (MQ=60)
                                                                                       GCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAT                                                               <  SRR19973063.3912185/150‑1 (MQ=60)
                                                                                       GCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAT                                                               <  SRR19973063.2834170/150‑1 (MQ=60)
                                                                                       GCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAT                                                               >  SRR19973063.3459926/1‑150 (MQ=60)
                                                                                       GCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAT                                                               <  SRR19973063.3308555/150‑1 (MQ=60)
                                                                                        ccgatctTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATT                                                              <  SRR19973063.2962272/143‑1 (MQ=60)
                                                                                        CAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATT                                                              >  SRR19973063.5066197/1‑150 (MQ=60)
                                                                                         AGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTG                                                             >  SRR19973063.397232/1‑150 (MQ=60)
                                                                                         AGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTG                                                             >  SRR19973063.2309432/1‑150 (MQ=60)
                                                                                         AGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTG                                                             >  SRR19973063.1300041/1‑150 (MQ=60)
                                                                                           GCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAA                                                           >  SRR19973063.2885903/1‑150 (MQ=60)
                                                                                               TGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTG                                                       >  SRR19973063.3332726/1‑150 (MQ=60)
                                                                                                GACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGT                                                      <  SRR19973063.2852123/150‑1 (MQ=60)
                                                                                                  CTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGC                                                    <  SRR19973063.4882900/150‑1 (MQ=60)
                                                                                                   TGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCTCGTCACTTCATTGAATGTGTGCA                                                   >  SRR19973063.1311269/1‑150 (MQ=60)
                                                                                                   TGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCTATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCA                                                   >  SRR19973063.4710570/1‑150 (MQ=60)
                                                                                                   TGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCA                                                   >  SRR19973063.610549/1‑150 (MQ=60)
                                                                                                        GTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACC                                              <  SRR19973063.1206657/150‑1 (MQ=60)
                                                                                                        GTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACC                                              <  SRR19973063.5104269/150‑1 (MQ=60)
                                                                                                          GCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAG                                            <  SRR19973063.2363370/150‑1 (MQ=60)
                                                                                                           CGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGA                                           <  SRR19973063.4821484/150‑1 (MQ=60)
                                                                                                            GCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGAC                                          <  SRR19973063.5179733/150‑1 (MQ=60)
                                                                                                            GCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGAC                                          <  SRR19973063.598118/150‑1 (MQ=60)
                                                                                                             CTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACA                                         >  SRR19973063.2949392/1‑150 (MQ=60)
                                                                                                              TCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAG                                        <  SRR19973063.1998440/150‑1 (MQ=60)
                                                                                                              TCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAG                                        <  SRR19973063.1998422/150‑1 (MQ=60)
                                                                                                               CATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAATCCAGACAGT                                       <  SRR19973063.827935/150‑1 (MQ=60)
                                                                                                                  CGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCC                                    <  SRR19973063.4111014/150‑1 (MQ=60)
                                                                                                                    ACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGC                                  <  SRR19973063.3801148/150‑1 (MQ=60)
                                                                                                                     TATTACGGATATGCGCGAATTGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCA                                 >  SRR19973063.1880344/1‑150 (MQ=60)
                                                                                                                     TATTAAGGATATGCGCGAATGGCGTTAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCA                                 >  SRR19973063.1880339/1‑150 (MQ=60)
                                                                                                                       TTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAA                               <  SRR19973063.1081487/150‑1 (MQ=60)
                                                                                                                        TACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAC                              <  SRR19973063.2191927/150‑1 (MQ=60)
                                                                                                                        TACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAC                              <  SRR19973063.2191933/150‑1 (MQ=60)
                                                                                                                           GGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGC                           <  SRR19973063.2105949/150‑1 (MQ=60)
                                                                                                                              TATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGG                        >  SRR19973063.972684/1‑150 (MQ=60)
                                                                                                                              TATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGG                        >  SRR19973063.3561294/1‑150 (MQ=60)
                                                                                                                                TGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG                      <  SRR19973063.3683828/150‑1 (MQ=60)
                                                                                                                                 GCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGA                     >  SRR19973063.4457411/1‑150 (MQ=60)
                                                                                                                                    CGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACA                  <  SRR19973063.3304747/150‑1 (MQ=60)
                                                                                                                                        TGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCC              <  SRR19973063.2446047/150‑1 (MQ=60)
                                                                                                                                        TGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCC              <  SRR19973063.2446050/150‑1 (MQ=60)
                                                                                                                                            GTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGC          >  SRR19973063.2650668/1‑150 (MQ=60)
                                                                                                                                            GTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATAGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGC          >  SRR19973063.2650724/1‑150 (MQ=60)
                                                                                                                                               AGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGG       <  SRR19973063.2525521/150‑1 (MQ=60)
                                                                                                                                               AGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGG       <  SRR19973063.3545695/150‑1 (MQ=60)
                                                                                                                                                 GAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCG     >  SRR19973063.4444590/1‑150 (MQ=60)
                                                                                                                                                  AGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGC    >  SRR19973063.2112603/1‑150 (MQ=60)
                                                                                                                                                  AGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGC    >  SRR19973063.2839688/1‑150 (MQ=60)
                                                                                                                                                    AGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAA  >  SRR19973063.2843044/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAA  >  CP009273/1122846‑1123143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑