Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,936,831	C→A	100%	noncoding (37/76 nt)	gltU →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,936,831	0	C	A	97.1%	288.5 / 2.7	137	noncoding (37/76 nt)	gltU	tRNA‑Glu
Reads supporting (aligned to +/- strand): ref base C (3/1); new base A (71/62); total (74/63)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 137 total aligned reads displayed.

GAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAA  >  CP009273/3936684‑3936905

gAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAAc                                                                          >  2:1269505/1‑150 (MQ=17)

 aaCCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAAcg                                                                         <  2:4872172/150‑1 (MQ=17)

     tGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAActgcgg                                                                     <  2:3578846/150‑1 (MQ=17)

      gCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGt                                                                    <  1:4139482/150‑1 (MQ=17)

       cGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTa                                                                   >  2:2751561/1‑150 (MQ=32)

       cGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTa                                                                   >  2:401471/1‑150 (MQ=17)

       cGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTa                                                                   >  2:4792282/1‑150 (MQ=17)

        ggTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTaa                                                                  >  1:1771140/1‑150 (MQ=17)

        ggTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTaa                                                                  >  1:1771177/1‑150 (MQ=17)

         gTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAAc                                                                 <  1:968139/150‑1 (MQ=17)

          ttGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACa                                                                <  2:2599853/150‑1 (MQ=17)

          ttGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACa                                                                >  1:844657/1‑150 (MQ=17)

           tGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAg                                                               >  1:2299508/1‑150 (MQ=17)

           tGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTATAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAg                                                               >  2:2036433/1‑150 (MQ=12)

            ggATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAgg                                                              >  2:1060494/1‑150 (MQ=17)

             gATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAggg                                                             <  1:3822303/150‑1 (MQ=17)

             gATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAggg                                                             >  2:191836/1‑150 (MQ=17)

             gATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAggg                                                             <  2:300047/150‑1 (MQ=17)

              aTCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAgggg                                                            >  2:4817693/1‑150 (MQ=17)

               tCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGt                                                           <  1:1188510/150‑1 (MQ=17)

                cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAATGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGtt                                                          >  1:3096559/1‑150 (MQ=17)

                cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGtt                                                          >  2:1710757/1‑150 (MQ=17)

                cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGtt                                                          >  2:1710758/1‑150 (MQ=17)

                cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGtt                                                          >  2:2361417/1‑150 (MQ=17)

                 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGGAACAGGGGTTc                                                         >  1:2659219/1‑150 (MQ=17)

                  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCg                                                        >  2:1909089/1‑150 (MQ=17)

                  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCg                                                        >  2:349559/1‑150 (MQ=17)

                   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGa                                                       >  1:4977148/1‑150 (MQ=17)

                   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGa                                                       >  2:2036246/1‑150 (MQ=17)

                   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGa                                                       >  1:1229370/1‑150 (MQ=17)

                     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAAt                                                     >  2:542863/1‑150 (MQ=17)

                     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAAt                                                     <  1:2626862/150‑1 (MQ=17)

                     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAAt                                                     >  2:1697805/1‑150 (MQ=17)

                     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAAt                                                     <  2:278689/150‑1 (MQ=17)

                       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATcc                                                   <  2:2553960/150‑1 (MQ=17)

                       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATcc                                                   >  2:3538515/1‑150 (MQ=17)

                        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATccc                                                  >  2:2824918/1‑150 (MQ=17)

                          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCt                                                <  1:3075244/150‑1 (MQ=17)

                          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCt                                                <  1:5130374/150‑1 (MQ=17)

                           cttaAAGAAGCGTTCTTTGCAGTGCTCAGACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTa                                               <  1:2105568/150‑1 (MQ=17)

                           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTa                                               <  1:5147775/150‑1 (MQ=17)

                           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTa                                               <  1:2960820/150‑1 (MQ=17)

                             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgg                                             <  1:2874215/150‑1 (MQ=17)

                             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgg                                             >  1:1038428/1‑150 (MQ=17)

                              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAggg                                            >  1:676912/1‑150 (MQ=17)

                                agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa                                          >  1:4896493/1‑150 (MQ=17)

                                agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa                                          <  2:2432197/150‑1 (MQ=17)

                                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACTCCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAc                                         <  1:3475688/150‑1 (MQ=17)

                                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAc                                         >  1:1350639/1‑150 (MQ=17)

                                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAc                                         >  2:4922422/1‑150 (MQ=17)

                                   agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGc                                       >  1:3956942/1‑150 (MQ=17)

                                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGcc                                      <  1:997910/150‑1 (MQ=17)

                                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGcc                                      <  1:2793540/150‑1 (MQ=17)

                                     cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCa                                     >  2:1102362/1‑150 (MQ=32)

                                     cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCa                                     >  2:3682649/1‑150 (MQ=32)

                                     cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCa                                     >  1:3497753/1‑150 (MQ=17)

                                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCAc                                    >  1:394753/1‑150 (MQ=17)

                                        tCTTTGCAGTGCTCACACAGTTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTTATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACtt                                  <  1:2846618/150‑1 (MQ=12)

                                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACtt                                  <  1:2929788/150‑1 (MQ=17)

                                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACtt                                  <  2:2345383/150‑1 (MQ=17)

                                         cTTTGCAGTGCTCACACAGATTGTCTGATCGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTg                                 <  2:855612/150‑1 (MQ=17)

                                         cTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTg                                 >  1:3383205/1‑150 (MQ=17)

                                          tttGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGc                                >  1:3764753/1‑150 (MQ=17)

                                            tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTg                              >  1:1041226/1‑150 (MQ=17)

                                            tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTg                              <  2:3263621/150‑1 (MQ=17)

                                            tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTg                              >  1:72705/1‑150 (MQ=17)

                                             gCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTgg                             >  1:3253458/1‑150 (MQ=17)

                                               cgTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                           >  1:1462973/2‑150 (MQ=17)

                                               cgTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                           >  1:2961774/2‑150 (MQ=17)

                                               cgTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                           >  1:2961816/2‑150 (MQ=17)

                                               aGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                           <  1:4157321/150‑1 (MQ=17)

                                               aGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                           >  1:1353843/1‑150 (MQ=17)

                                               aGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                           >  2:1303524/1‑150 (MQ=17)

                                                gTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGttt                          <  2:2845151/150‑1 (MQ=17)

                                                gTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGttt                          <  2:2845224/150‑1 (MQ=17)

                                                 tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCANGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                         >  1:2680705/1‑150 (MQ=17)

                                                   cTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgtg                       >  1:3329919/1‑150 (MQ=17)

                                                   cTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgtg                       <  1:4272878/150‑1 (MQ=17)

                                                     cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag                     <  2:581890/150‑1 (MQ=17)

                                                     cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag                     <  2:3103913/150‑1 (MQ=17)

                                                     cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag                     >  2:4171174/1‑150 (MQ=17)

                                                      acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                    <  1:4629357/150‑1 (MQ=17)

                                                      acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                    <  2:4543279/150‑1 (MQ=17)

                                                       cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgcgagtg                   <  2:3248438/150‑1 (MQ=17)

                                                         caGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaa                 >  1:1966879/1‑150 (MQ=17)

                                                         caGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaa                 <  2:3711254/150‑1 (MQ=17)

                                                          aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa                >  2:1140292/1‑150 (MQ=17)

                                                          aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa                <  2:1207904/150‑1 (MQ=17)

                                                          aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa                <  2:3367120/150‑1 (MQ=17)

                                                           gATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAg               >  1:3191433/1‑150 (MQ=17)

                                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt              <  1:101281/150‑1 (MQ=32)

                                                             ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTc             <  1:1162276/150‑1 (MQ=17)

                                                              tGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCa            <  1:4167324/150‑1 (MQ=17)

                                                                    aTAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGcc      >  2:4542881/1‑150 (MQ=17)

                                                                     tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGccc     <  2:3659024/150‑2 (MQ=17)

                                                                     tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGccc     <  2:5056037/150‑2 (MQ=17)

                                                                     tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt     >  2:1766872/1‑150 (MQ=17)

                                                                     tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCGGCCt     <  1:2735170/150‑1 (MQ=17)

                                                                      aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACATGCCtt    <  2:3019670/150‑1 (MQ=17)

                                                                        gAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCACTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGTCGGTATCAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCTCCTGCCTTaa  <  2:2350011/150‑1 (MQ=11)

GAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAA  >  CP009273/3936684‑3936905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A