Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 201,962 A→T 100% D117V (GAT→GTT)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273201,9620AT98.7% 704.5 / ‑0.4 227D117V (GAT→GTT) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (108/115);  total (109/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

ATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCG  >  CP009273/201818‑202106
                                                                                                                                                |                                                                                                                                                
aTTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCatcgttcg                                                                                                                                               >  2:3119980/1‑148 (MQ=255)
 ttttAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTcgc                                                                                                                                              >  2:692670/1‑148 (MQ=255)
    tAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGAc                                                                                                                                           <  2:1438660/148‑1 (MQ=255)
      aCGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTg                                                                                                                                         >  1:3235443/1‑148 (MQ=255)
         tCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCt                                                                                                                                      <  1:3009037/148‑1 (MQ=255)
              tGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCg                                                                                                                                 >  1:3438490/1‑148 (MQ=255)
                    ctgctgGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTaa                                                                                                                           <  1:1148108/148‑1 (MQ=255)
                         gggTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGaa                                                                                                                      <  2:1157789/148‑1 (MQ=255)
                         gggTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGaa                                                                                                                      >  1:3512485/1‑148 (MQ=255)
                           gTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAgg                                                                                                                    <  1:224315/148‑1 (MQ=255)
                            tgatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAggg                                                                                                                   >  1:3126748/1‑148 (MQ=255)
                             gatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGt                                                                                                                  <  1:1518212/148‑1 (MQ=255)
                             gatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGt                                                                                                                  >  2:238934/1‑148 (MQ=255)
                               tgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTg                                                                                                                <  1:2830374/148‑1 (MQ=255)
                                gaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGa                                                                                                               >  1:258240/1‑148 (MQ=255)
                                  gTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATc                                                                                                             <  2:3159218/148‑1 (MQ=255)
                                  gTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATc                                                                                                             <  2:3239623/148‑1 (MQ=255)
                                    tAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCct                                                                                                           <  2:1342205/148‑1 (MQ=255)
                                     aaCCCACCTGACGGTACTGGCGGCGAACATGACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTTTAAGCGCGGGTACGGTGCCGCCGGGCGGATCATCGTTCGCTACTGGCTTATAGATTTAAACGAAGGGTTGATCctt                                                                                                          <  1:3106867/148‑1 (MQ=255)
                                        ccACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCcttctt                                                                                                       >  1:1034177/1‑148 (MQ=255)
                                        ccACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCcttctt                                                                                                       >  1:1932223/1‑148 (MQ=255)
                                         cACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCttt                                                                                                      >  1:3248327/1‑148 (MQ=255)
                                             tGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCcgg                                                                                                  <  1:1571391/148‑1 (MQ=255)
                                               aCGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCcggcg                                                                                                >  1:976423/1‑148 (MQ=255)
                                                     cTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCa                                                                                          <  2:3207681/148‑1 (MQ=255)
                                                      tGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACTGTGACGCCGGGCCGATCATCGTTGGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCAt                                                                                         <  1:3023097/148‑1 (MQ=255)
                                                      tGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCAt                                                                                         >  2:3393598/1‑148 (MQ=255)
                                                        gcggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATgg                                                                                       >  1:12024/1‑148 (MQ=255)
                                                        gcggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATgg                                                                                       >  2:430051/1‑148 (MQ=255)
                                                         cggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATggg                                                                                      <  2:642/148‑1 (MQ=255)
                                                           gcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCg                                                                                    <  1:1445017/148‑1 (MQ=255)
                                                              aaCAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACg                                                                                 >  2:2720704/1‑148 (MQ=255)
                                                               aCAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGt                                                                                >  1:2874180/1‑148 (MQ=255)
                                                               aCAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGt                                                                                >  1:824692/1‑148 (MQ=255)
                                                                  aTACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCgg                                                                             >  1:2901773/1‑148 (MQ=255)
                                                                    aCCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGAc                                                                           >  2:2158200/1‑148 (MQ=255)
                                                                    aCCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGAc                                                                           >  1:2098439/1‑148 (MQ=255)
                                                                     ccGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACg                                                                          >  1:3250074/1‑148 (MQ=255)
                                                                      cGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGc                                                                         <  2:2088454/148‑1 (MQ=255)
                                                                       ggCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCa                                                                        >  1:1248310/1‑148 (MQ=255)
                                                                          tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTc                                                                     >  1:2250840/1‑148 (MQ=255)
                                                                          tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTc                                                                     >  1:2250994/1‑148 (MQ=255)
                                                                          tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTc                                                                     <  2:2240763/148‑1 (MQ=255)
                                                                            tCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTAt                                                                                                                                   >  2:3382216/1‑84 (MQ=255)
                                                                            tCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTAt                                                                                                                                   <  1:649081/84‑1 (MQ=255)
                                                                               gAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTg                                                                >  1:1774000/1‑148 (MQ=255)
                                                                               gAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTg                                                                >  1:2630322/1‑148 (MQ=255)
                                                                                 aTCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCg                                                              <  1:2753960/148‑1 (MQ=255)
                                                                                 aTCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCg                                                              <  2:2952908/148‑1 (MQ=255)
                                                                                 aTCGGACGTTGCTGATCTCAACAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCg                                                              <  1:1641475/148‑1 (MQ=255)
                                                                                   cTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTg                                                            <  2:1862190/148‑1 (MQ=255)
                                                                                    tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                           <  2:2034902/148‑1 (MQ=255)
                                                                                    tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                           >  2:3480652/1‑148 (MQ=255)
                                                                                    tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                           <  2:2613503/148‑1 (MQ=255)
                                                                                    tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGGTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                           <  2:2509699/148‑1 (MQ=255)
                                                                                       cGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTaa                                                        <  1:1105014/148‑1 (MQ=255)
                                                                                        gTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAAc                                                       >  1:2924773/1‑148 (MQ=255)
                                                                                            cTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcg                                                   <  2:2257666/148‑1 (MQ=255)
                                                                                              gATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcg                                                 >  2:2931481/1‑148 (MQ=255)
                                                                                              gATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcg                                                 <  1:3280222/148‑1 (MQ=255)
                                                                                              gATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcg                                                 <  1:3280800/148‑1 (MQ=255)
                                                                                               aTCTCAAAAGCGTATCAGCGCGGGTCCGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcgc                                                >  2:186920/1‑148 (MQ=255)
                                                                                               aTCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcgc                                                >  1:3014128/1‑148 (MQ=255)
                                                                                                 ctcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTg                                              >  2:2899865/1‑148 (MQ=255)
                                                                                                  tcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTgg                                             <  1:2457095/148‑1 (MQ=255)
                                                                                                  tcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTgg                                             <  2:1405469/148‑1 (MQ=255)
                                                                                                  tcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCACGCTgg                                             >  2:1366881/1‑148 (MQ=255)
                                                                                                   cAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGt                                            <  1:1271166/148‑1 (MQ=255)
                                                                                                   cAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGt                                            <  2:2076206/148‑1 (MQ=255)
                                                                                                     aaaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAg                                          >  1:994596/1‑148 (MQ=255)
                                                                                                      aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                         <  2:362125/148‑1 (MQ=255)
                                                                                                      aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                         >  1:2021155/1‑148 (MQ=255)
                                                                                                      aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                         >  2:671338/1‑148 (MQ=255)
                                                                                                      aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                         <  1:448083/148‑1 (MQ=255)
                                                                                                       aGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGAt                                        <  2:3117594/148‑1 (MQ=255)
                                                                                                             tCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgtgt                                  <  2:3015734/148‑1 (MQ=255)
                                                                                                                 cgcgGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTcg                              <  2:2171308/148‑1 (MQ=255)
                                                                                                                   cgGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTcgcg                            <  2:1222505/148‑1 (MQ=255)
                                                                                                                     ggTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtt                          <  1:3339525/148‑1 (MQ=255)
                                                                                                                      gTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGttt                         >  1:2359/1‑148 (MQ=255)
                                                                                                                      gTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGttt                         <  2:152471/148‑1 (MQ=255)
                                                                                                                      gTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGttt                         <  1:2793343/148‑1 (MQ=255)
                                                                                                                       tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                        <  1:1732838/148‑1 (MQ=255)
                                                                                                                       tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                        <  2:2690752/148‑1 (MQ=255)
                                                                                                                       tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                        <  1:591268/148‑1 (MQ=255)
                                                                                                                       tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                        <  2:740002/148‑1 (MQ=255)
                                                                                                                          ggTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATg                     <  2:834994/148‑1 (MQ=255)
                                                                                                                            tGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaa                   >  1:3197456/1‑148 (MQ=255)
                                                                                                                             gccgccGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaag                  <  1:2770454/148‑1 (MQ=255)
                                                                                                                              ccgccgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTCACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaaga                 >  2:2196083/1‑148 (MQ=255)
                                                                                                                                   gggCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACt            <  2:1266298/148‑1 (MQ=255)
                                                                                                                                      ccGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTcc         <  1:1508198/148‑1 (MQ=255)
                                                                                                                                       cGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTccc        <  1:2720704/148‑1 (MQ=255)
                                                                                                                                         atcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCgg      >  2:2245462/1‑148 (MQ=255)
                                                                                                                                          tcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGa     >  2:2803185/1‑148 (MQ=255)
                                                                                                                                          tcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGa     >  1:1378070/1‑148 (MQ=255)
                                                                                                                                             tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg  >  1:1412192/1‑148 (MQ=255)
                                                                                                                                             tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg  <  1:430051/148‑1 (MQ=255)
                                                                                                                                             tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg  >  2:1599270/1‑148 (MQ=255)
                                                                                                                                             tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg  >  1:821794/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCG  >  CP009273/201818‑202106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTAT  >  CP009273/201814‑202110
                                                                                                                                                    |                                                                                                                                                    
GCAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTT                                                                                                                                                     <  SRR19973065.2082810/150‑1 (MQ=60)
 CAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTC                                                                                                                                                    <  SRR19973065.489633/150‑1 (MQ=60)
        TAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTG                                                                                                                                             >  SRR19973065.3254912/1‑150 (MQ=60)
             TCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTA                                                                                                                                        <  SRR19973065.3027461/150‑1 (MQ=60)
               CAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATC                                                                                                                                      <  SRR19973065.3333436/150‑1 (MQ=60)
               CAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATC                                                                                                                                      <  SRR19973065.742230/150‑1 (MQ=60)
                cccccccccccccgagattctggcatcagtgactggagttcagacgtgtgctcttccgatctTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCG                                                                                                                                     <  SRR19973065.653352/88‑1 (MQ=60)
                cacccccccccccgcgattctggcatcagtgactggagttcagacgtgtgctcttccgatctTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCG                                                                                                                                     <  SRR19973065.3402896/88‑1 (MQ=60)
                AGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCG                                                                                                                                     >  SRR19973065.3459465/1‑150 (MQ=60)
                    CGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATT                                                                                                                                 <  SRR19973065.3068233/150‑1 (MQ=60)
                     GACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTA                                                                                                                                >  SRR19973065.1274645/1‑150 (MQ=60)
                     GACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTA                                                                                                                                >  SRR19973065.2364301/1‑150 (MQ=60)
                     GACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTA                                                                                                                                >  SRR19973065.216870/1‑150 (MQ=60)
                        CTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAAC                                                                                                                             <  SRR19973065.1155510/150‑1 (MQ=60)
                           CTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAA                                                                                                                          >  SRR19973065.3533784/1‑150 (MQ=60)
                              GGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGG                                                                                                                       >  SRR19973065.3145783/1‑150 (MQ=60)
                              GGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGG                                                                                                                       >  SRR19973065.1904354/1‑150 (MQ=60)
                               GTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGT                                                                                                                      <  SRR19973065.225972/150‑1 (MQ=60)
                               GTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGT                                                                                                                      <  SRR19973065.226032/150‑1 (MQ=60)
                               GTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGT                                                                                                                      >  SRR19973065.3286738/1‑150 (MQ=60)
                                TGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTT                                                                                                                     <  SRR19973065.2793030/150‑1 (MQ=60)
                                 GATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTG                                                                                                                    <  SRR19973065.1528143/150‑1 (MQ=60)
                                  ATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGA                                                                                                                   >  SRR19973065.260030/1‑150 (MQ=60)
                                   TGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGAT                                                                                                                  <  SRR19973065.2847830/150‑1 (MQ=60)
                                        TAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTC                                                                                                             >  SRR19973065.3068380/1‑150 (MQ=60)
                                         AACCCACCTGACGGTACTGGCGGCGAACATGACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTTTAAGCGCGGGTACGGTGCCGCCGGGCGGATCATCGTTCGCTACTGGCTTATAGATTTAAACGAAGGGTTGATCCTTCT                                                                                                            <  SRR19973065.3125821/150‑1 (MQ=60)
                                         AACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGTGTTGATCCTTCT                                                                                                            >  SRR19973065.3028154/1‑150 (MQ=60)
                                          ACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTT                                                                                                           >  SRR19973065.1944874/1‑150 (MQ=60)
                                          ACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTT                                                                                                           >  SRR19973065.1041023/1‑150 (MQ=60)
                                          ACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTT                                                                                                           >  SRR19973065.1152546/1‑150 (MQ=60)
                                           CCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTT                                                                                                          >  SRR19973065.3040773/1‑150 (MQ=60)
                                           CCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTT                                                                                                          >  SRR19973065.3267947/1‑150 (MQ=60)
                                           CCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTT                                                                                                          >  SRR19973065.3268013/1‑150 (MQ=60)
                                             CACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCC                                                                                                        <  SRR19973065.3138989/150‑1 (MQ=60)
                                                 TGACGGTACTGGCGGCGAACAATACCGTCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCCGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCG                                                                                                    <  SRR19973065.1581845/150‑1 (MQ=60)
                                                 TGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCG                                                                                                    <  SRR19973065.1582601/150‑1 (MQ=60)
                                                 TGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCG                                                                                                    >  SRR19973065.1211580/1‑150 (MQ=60)
                                                 TGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCG                                                                                                    >  SRR19973065.2971800/1‑150 (MQ=60)
                                                 TGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCG                                                                                                    >  SRR19973065.982908/1‑150 (MQ=60)
                                                     GGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACG                                                                                                <  SRR19973065.1886979/150‑1 (MQ=60)
                                                          TGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACTGTGACGCCGGGCCGATCATCGTTGGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGG                                                                                           <  SRR19973065.3041624/150‑1 (MQ=60)
                                                          TGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGG                                                                                           >  SRR19973065.12201/1‑150 (MQ=60)
                                                               GCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGAC                                                                                      <  SRR19973065.1454389/150‑1 (MQ=60)
                                                               GCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGAC                                                                                      <  SRR19973065.1454360/150‑1 (MQ=60)
                                                                 GAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGT                                                                                    >  SRR19973065.830101/1‑150 (MQ=60)
                                                                 GAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGT                                                                                    >  SRR19973065.2891789/1‑150 (MQ=60)
                                                                    CAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGG                                                                                 >  SRR19973065.152952/1‑150 (MQ=60)
                                                                    CAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGG                                                                                 >  SRR19973065.2919473/1‑150 (MQ=60)
                                                                     AATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTTCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGA                                                                                >  SRR19973065.1491957/1‑150 (MQ=60)
                                                                      ATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGAC                                                                               >  SRR19973065.2111935/1‑150 (MQ=60)
                                                                      ATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGAC                                                                               >  SRR19973065.1949694/1‑150 (MQ=60)
                                                                      ATACCGGCTATCAGAATCTGACGTACCAGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGAC                                                                               >  SRR19973065.3510513/1‑150 (MQ=60)
                                                                       TACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACG                                                                              >  SRR19973065.3269714/1‑150 (MQ=60)
                                                                        ACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGC                                                                             >  SRR19973065.987133/1‑150 (MQ=60)
                                                                         CCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCA                                                                            >  SRR19973065.1256532/1‑150 (MQ=60)
                                                                            GCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTC                                                                         >  SRR19973065.2265108/1‑150 (MQ=60)
                                                                            GCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTC                                                                         >  SRR19973065.2265134/1‑150 (MQ=60)
                                                                            GCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTC                                                                         >  SRR19973065.2265262/1‑150 (MQ=60)
                                                                             CTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCT                                                                        <  SRR19973065.2630836/150‑1 (MQ=60)
                                                                             CTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCT                                                                        <  SRR19973065.2630860/150‑1 (MQ=60)
                                                                              TCTCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTT                                                                       <  SRR19973065.3239068/150‑1 (MQ=60)
                                                                              TATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTT                                                                       <  SRR19973065.3239125/150‑1 (MQ=60)
                                                                               ATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTT                                                                      <  SRR19973065.854866/150‑1 (MQ=60)
                                                                               ATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTT                                                                      >  SRR19973065.3275288/1‑150 (MQ=60)
                                                                                 CAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTG                                                                    >  SRR19973065.1785608/1‑150 (MQ=60)
                                                                                 CAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTG                                                                    >  SRR19973065.2879147/1‑150 (MQ=60)
                                                                                 CAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTG                                                                    >  SRR19973065.2646586/1‑150 (MQ=60)
                                                                                 CAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTG                                                                    <  SRR19973065.1426709/150‑1 (MQ=60)
                                                                                     ATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTG                                                                >  SRR19973065.339024/1‑150 (MQ=60)
                                                                                     ATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTG                                                                <  SRR19973065.2771282/150‑1 (MQ=60)
                                                                                     ATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTG                                                                <  SRR19973065.2771092/150‑1 (MQ=60)
                                                                                     ATCGGACGTTGCTGATCTCAACAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTG                                                                <  SRR19973065.1652499/150‑1 (MQ=60)
                                                                                          ACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAAC                                                           >  SRR19973065.2942541/1‑150 (MQ=60)
                                                                                          ACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAAC                                                           >  SRR19973065.2786007/1‑150 (MQ=60)
                                                                                          ACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCAGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAAC                                                           >  SRR19973065.2941738/1‑150 (MQ=60)
                                                                                           CGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACA                                                          <  SRR19973065.1112259/150‑1 (MQ=60)
                                                                                              TGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCG                                                       <  SRR19973065.1187559/150‑1 (MQ=60)
                                                                                                 TGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGC                                                    >  SRR19973065.3032585/1‑150 (MQ=60)
                                                                                                  GATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCT                                                   <  SRR19973065.3300158/150‑1 (MQ=60)
                                                                                                  GATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCT                                                   <  SRR19973065.3300453/150‑1 (MQ=60)
                                                                                                  GATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCT                                                   <  SRR19973065.3300743/150‑1 (MQ=60)
                                                                                                   ATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTG                                                  >  SRR19973065.1576588/1‑150 (MQ=60)
                                                                                                     CTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGT                                                <  SRR19973065.639843/150‑1 (MQ=60)
                                                                                                     CTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCGGGT                                                <  SRR19973065.639940/150‑1 (MQ=60)
                                                                                                      TCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTA                                               <  SRR19973065.2472580/150‑1 (MQ=60)
                                                                                                       CAAAAGCGTATCAGCGCGGGTGCGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCGGGTAG                                              <  SRR19973065.1691645/150‑1 (MQ=60)
                                                                                                       CAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAG                                              >  SRR19973065.1001209/1‑150 (MQ=60)
                                                                                                       CAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAG                                              <  SRR19973065.1279550/150‑1 (MQ=60)
                                                                                                       CAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAG                                              <  SRR19973065.1279665/150‑1 (MQ=60)
                                                                                                        AAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGA                                             >  SRR19973065.2034160/1‑150 (MQ=60)
                                                                                                         AAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGAT                                            >  SRR19973065.395477/1‑150 (MQ=60)
                                                                                                         AAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTCCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGAT                                            >  SRR19973065.2282048/1‑150 (MQ=60)
                                                                                                          AAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATG                                           <  SRR19973065.3167493/150‑1 (MQ=60)
                                                                                                          AAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATG                                           <  SRR19973065.451194/150‑1 (MQ=60)
                                                                                                          AAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATG                                           <  SRR19973065.451162/150‑1 (MQ=60)
                                                                                                            GCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAG                                         <  SRR19973065.828130/150‑1 (MQ=60)
                                                                                                              GTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTG                                       >  SRR19973065.1078111/1‑150 (MQ=60)
                                                                                                              GTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTG                                       >  SRR19973065.2841286/1‑150 (MQ=60)
                                                                                                                ATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGACGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTG                                     >  SRR19973065.917696/1‑150 (MQ=60)
                                                                                                                ATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTG                                     >  SRR19973065.1371512/1‑150 (MQ=60)
                                                                                                                  CAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTC                                   <  SRR19973065.1618108/150‑1 (MQ=60)
                                                                                                                   AGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCG                                  <  SRR19973065.2716854/150‑1 (MQ=60)
                                                                                                                   AGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCG                                  >  SRR19973065.3009856/1‑150 (MQ=60)
                                                                                                                       CGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTT                              <  SRR19973065.2904566/150‑1 (MQ=60)
                                                                                                                        GGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCCGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTT                             >  SRR19973065.3263386/1‑150 (MQ=60)
                                                                                                                        GGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTT                             >  SRR19973065.2385/1‑150 (MQ=60)
                                                                                                                        GGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTT                             >  SRR19973065.2476826/1‑150 (MQ=60)
                                                                                                                        GGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTT                             <  SRR19973065.2166891/150‑1 (MQ=60)
                                                                                                                         GGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTT                            <  SRR19973065.3359923/150‑1 (MQ=60)
                                                                                                                          GTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTA                           <  SRR19973065.2810655/150‑1 (MQ=60)
                                                                                                                           TACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTAT                          <  SRR19973065.1744306/150‑1 (MQ=60)
                                                                                                                           TACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTAT                          <  SRR19973065.594929/150‑1 (MQ=60)
                                                                                                                              GGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAA                       >  SRR19973065.3216721/1‑150 (MQ=60)
                                                                                                                                 GCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAA                    <  SRR19973065.2787662/150‑1 (MQ=60)
                                                                                                                                      CGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTT               >  SRR19973065.2853350/1‑150 (MQ=60)
                                                                                                                                        GGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCC             <  SRR19973065.1784820/150‑1 (MQ=60)
                                                                                                                                         GCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCC            <  SRR19973065.2439733/150‑1 (MQ=60)
                                                                                                                                         GCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCC            <  SRR19973065.1419197/150‑1 (MQ=60)
                                                                                                                                          CCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCG           <  SRR19973065.1174653/150‑1 (MQ=60)
                                                                                                                                          CCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCG           >  SRR19973065.552580/1‑150 (MQ=60)
                                                                                                                                          CCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCG           <  SRR19973065.1518004/150‑1 (MQ=60)
                                                                                                                                           CGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGG          <  SRR19973065.2737673/150‑1 (MQ=60)
                                                                                                                                           CGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGG          <  SRR19973065.2737625/150‑1 (MQ=60)
                                                                                                                                            GATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGA         >  SRR19973065.1387154/1‑150 (MQ=60)
                                                                                                                                            GATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGA         >  SRR19973065.2043229/1‑150 (MQ=60)
                                                                                                                                               CATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCG      <  SRR19973065.433480/150‑1 (MQ=60)
                                                                                                                                               CATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCG      >  SRR19973065.827189/1‑150 (MQ=60)
                                                                                                                                               CATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCG      >  SRR19973065.1421422/1‑150 (MQ=60)
                                                                                                                                                 TCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCT    <  SRR19973065.433036/150‑1 (MQ=60)
                                                                                                                                                   GTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTAT  >  SRR19973065.748448/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
GCAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTAT  >  CP009273/201814‑202110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑