Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 319,381 G→T 100% D445Y (GAC→TAC)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273319,3810GT99.0% 649.2 / ‑2.0 200D445Y (GAC→TAC) ykgFferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (91/106);  total (92/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

AAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGA  >  CP009273/319237‑319528
                                                                                                                                                |                                                                                                                                                   
aaaGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACt                                                                                                                                                  >  2:177804/1‑148 (MQ=255)
   gCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACtgga                                                                                                                                               >  2:228838/1‑148 (MQ=255)
     agagCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACtggatg                                                                                                                                             >  1:2819505/1‑148 (MQ=255)
       agCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACtggatgga                                                                                                                                           >  2:2843118/1‑148 (MQ=255)
       agCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACtggatgga                                                                                                                                           <  1:2056182/148‑1 (MQ=255)
       agCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACtggatgga                                                                                                                                           <  1:1838816/148‑1 (MQ=255)
           aCGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCa                                                                                                                                       >  1:640122/1‑148 (MQ=255)
                cGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGa                                                                                                                                  <  1:1029708/148‑1 (MQ=255)
                  aTAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATc                                                                                                                                <  2:793449/148‑1 (MQ=255)
                   tAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCt                                                                                                                               >  1:2463831/1‑148 (MQ=255)
                    aaaaaTGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCtt                                                                                                                              <  1:2485197/148‑1 (MQ=255)
                       aaTGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCt                                                                                                                           >  1:3339161/1‑148 (MQ=255)
                       aaTGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGCATTGAAGCACTCGATCTTCCt                                                                                                                           >  1:1607104/1‑148 (MQ=255)
                         tGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGa                                                                                                                         >  1:1637273/1‑148 (MQ=255)
                          gTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGaa                                                                                                                        >  2:596391/1‑148 (MQ=255)
                             cGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCt                                                                                                                     >  2:2740626/1‑148 (MQ=255)
                              gCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTg                                                                                                                    <  1:1104404/148‑1 (MQ=255)
                              gCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTg                                                                                                                    <  1:1955082/148‑1 (MQ=255)
                               cTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGa                                                                                                                   <  1:3153794/148‑1 (MQ=255)
                                ttATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGAc                                                                                                                  >  2:161942/1‑148 (MQ=255)
                                 tATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACg                                                                                                                 <  2:3088532/148‑1 (MQ=255)
                                 tATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACg                                                                                                                 >  2:733025/1‑148 (MQ=255)
                                   tGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGga                                                                                                               <  2:1807542/148‑1 (MQ=255)
                                    gCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGACGCACGCGATCTTCCTGAAGCTGACGgag                                                                                                              >  2:2060432/1‑148 (MQ=255)
                                    gCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgag                                                                                                              <  2:3021501/148‑1 (MQ=255)
                                    gCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgag                                                                                                              >  1:1276958/1‑148 (MQ=255)
                                     ccAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgaga                                                                                                             <  2:2887968/148‑1 (MQ=255)
                                      cAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgagag                                                                                                            <  2:2575611/148‑1 (MQ=255)
                                       aaTAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgagaga                                                                                                           >  1:1187295/1‑148 (MQ=255)
                                       aaTAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgagaga                                                                                                           >  2:1197220/1‑148 (MQ=255)
                                        aTAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGagagag                                                                                                          >  1:3010105/1‑148 (MQ=255)
                                         tAGTCCTCCAGGAGTGTGGAACGTCGGGCTGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGt                                                                                                         <  2:156652/148‑1 (MQ=255)
                                            tCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTc                                                                                                      <  1:1648732/148‑1 (MQ=255)
                                               tCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGt                                                                                                   <  1:617174/148‑1 (MQ=255)
                                                ccAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTa                                                                                                  >  2:520528/1‑148 (MQ=255)
                                                  aGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGt                                                                                                <  2:639882/148‑1 (MQ=255)
                                                  aGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGt                                                                                                >  2:2781108/1‑148 (MQ=255)
                                                  aGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGt                                                                                                <  2:2956392/148‑1 (MQ=255)
                                                    gATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTg                                                                                              >  2:1388879/1‑148 (MQ=255)
                                                     aTTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTgg                                                                                             <  2:926984/148‑1 (MQ=255)
                                                      ttGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGt                                                                                            <  2:2733718/148‑1 (MQ=255)
                                                         tgGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTa                                                                                         >  1:2936429/1‑148 (MQ=255)
                                                           gAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAg                                                                                       >  1:2213406/1‑148 (MQ=255)
                                                                tCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACa                                                                                  <  2:3286152/148‑1 (MQ=255)
                                                                 cGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACAt                                                                                 <  2:459741/148‑1 (MQ=255)
                                                                  gggATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATc                                                                                <  2:444717/148‑1 (MQ=255)
                                                                  gggATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATc                                                                                >  2:1489864/1‑148 (MQ=255)
                                                                       gatgGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGgcg                                                                           >  2:2584600/1‑148 (MQ=255)
                                                                           gCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAgg                                                                       >  2:1631172/1‑148 (MQ=255)
                                                                              ggTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGgaga                                                                    <  1:60589/148‑1 (MQ=255)
                                                                               gTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGaa                                                                   <  2:851158/148‑1 (MQ=255)
                                                                                tGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGaaa                                                                  >  2:1511029/1‑148 (MQ=255)
                                                                                 gCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGaaaa                                                                 <  2:2315335/148‑1 (MQ=255)
                                                                                    cATGCGGCAAGCTGGTTTATCAATGGCGTCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGa                                                              <  1:245534/148‑1 (MQ=255)
                                                                                           cAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATa                                                       >  2:2613037/1‑148 (MQ=255)
                                                                                            aaGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATaa                                                      <  2:3301735/148‑1 (MQ=255)
                                                                                             aGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAAt                                                     >  2:1655597/1‑148 (MQ=255)
                                                                                             aGCTGGTTGATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAAt                                                     <  1:2286176/148‑1 (MQ=255)
                                                                                              gCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATc                                                    >  1:2703581/1‑148 (MQ=255)
                                                                                               cTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCg                                                   <  2:1276958/148‑1 (MQ=255)
                                                                                                 ggTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAg                                                 <  1:3188429/148‑1 (MQ=255)
                                                                                                  gTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAgg                                                >  2:340843/1‑148 (MQ=255)
                                                                                                  gTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAgg                                                <  1:2538420/148‑1 (MQ=255)
                                                                                                   tttATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGc                                               >  1:3090424/1‑148 (MQ=255)
                                                                                                   tttATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGc                                               >  2:480475/1‑148 (MQ=255)
                                                                                                    ttATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCg                                              >  2:3173768/1‑148 (MQ=255)
                                                                                                      aTCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGaa                                            <  1:2648710/148‑1 (MQ=255)
                                                                                                         aaTGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAttt                                         >  2:681362/1‑148 (MQ=255)
                                                                                                          aTGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAtttt                                        >  2:2974795/1‑148 (MQ=255)
                                                                                                          aTGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAtttt                                        <  1:829636/148‑1 (MQ=255)
                                                                                                          aTGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAtttt                                        <  1:3227290/148‑1 (MQ=255)
                                                                                                           tGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAttttt                                       >  1:974632/1‑148 (MQ=255)
                                                                                                             gcggcAAAACACCACTCAAATTTGGCTCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGa                                     <  2:1019030/148‑1 (MQ=255)
                                                                                                                 cAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATaa                                 <  2:2848458/148‑1 (MQ=255)
                                                                                                                 cAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATaa                                 >  2:1842345/1‑148 (MQ=255)
                                                                                                                 cAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATaa                                 <  1:571890/148‑1 (MQ=255)
                                                                                                                   aaaCACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACg                               <  2:2010674/148‑1 (MQ=255)
                                                                                                                    aaCACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGt                              <  1:733025/148‑1 (MQ=255)
                                                                                                                     acacCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGtt                             >  2:2432906/1‑148 (MQ=255)
                                                                                                                      caccacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTg                            <  1:1658159/148‑1 (MQ=255)
                                                                                                                      caccacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTg                            <  1:1935784/148‑1 (MQ=255)
                                                                                                                      caccacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTg                            <  2:1250040/148‑1 (MQ=255)
                                                                                                                       accacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGc                           >  2:1573326/1‑148 (MQ=255)
                                                                                                                        ccacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCt                          <  1:2408202/148‑1 (MQ=255)
                                                                                                                        ccacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCt                          >  2:1544644/1‑148 (MQ=255)
                                                                                                                         cacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTc                         <  1:2781108/148‑1 (MQ=255)
                                                                                                                         cacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTc                         >  2:1299771/1‑148 (MQ=255)
                                                                                                                          acTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCa                        <  1:211789/148‑1 (MQ=255)
                                                                                                                            tCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAgg                      <  1:1395138/148‑1 (MQ=255)
                                                                                                                            tCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAgg                      >  2:3389485/1‑148 (MQ=255)
                                                                                                                             cAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGc                     >  1:1891812/1‑148 (MQ=255)
                                                                                                                              aaaTTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCa                    <  2:1187295/148‑1 (MQ=255)
                                                                                                                               aaTTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCAc                   >  1:2836869/1‑148 (MQ=255)
                                                                                                                                aTTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACt                  <  1:81019/148‑1 (MQ=255)
                                                                                                                                 tttGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTg                 <  1:2949865/148‑1 (MQ=255)
                                                                                                                                  ttGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTgg                >  2:1834218/1‑148 (MQ=255)
                                                                                                                                    ggCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGt              <  2:1253381/148‑1 (MQ=255)
                                                                                                                                          aTTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCg        >  1:373217/1‑148 (MQ=255)
                                                                                                                                             aGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTg     <  1:1962198/148‑1 (MQ=255)
                                                                                                                                             aGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTg     <  1:2154190/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGA  >  CP009273/319237‑319528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  CP009273/319232‑319530
                                                                                                                                                     |                                                                                                                                                     
CCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGTCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCT                                                                                                                                                       <  SRR19973065.3432633/150‑1 (MQ=60)
CCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCT                                                                                                                                                       >  SRR19973065.3337002/1‑150 (MQ=60)
 CGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTA                                                                                                                                                      >  SRR19973065.3260084/1‑150 (MQ=60)
    AAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTG                                                                                                                                                   >  SRR19973065.988691/1‑150 (MQ=60)
      AAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGA                                                                                                                                                 >  SRR19973065.2118276/1‑150 (MQ=60)
        GCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATG                                                                                                                                               >  SRR19973065.2836921/1‑150 (MQ=60)
        GCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATG                                                                                                                                               >  SRR19973065.2836922/1‑150 (MQ=60)
            AGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAG                                                                                                                                           <  SRR19973065.2069311/150‑1 (MQ=60)
            AGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAG                                                                                                                                           <  SRR19973065.1850940/150‑1 (MQ=60)
            AGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTAGCGCGATTAGCTACTGGATGGAAG                                                                                                                                           <  SRR19973065.1907523/150‑1 (MQ=60)
              CAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCA                                                                                                                                         >  SRR19973065.644319/1‑150 (MQ=60)
              CAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCA                                                                                                                                         >  SRR19973065.643848/1‑150 (MQ=60)
                     CGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATC                                                                                                                                  <  SRR19973065.1036527/150‑1 (MQ=60)
                     CGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATC                                                                                                                                  >  SRR19973065.3028360/1‑150 (MQ=60)
                     CGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATATGGCGCGATTAGCTCCTGGATGGAAGCACGCGATC                                                                                                                                  >  SRR19973065.447793/1‑150 (MQ=60)
                      GATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCT                                                                                                                                 >  SRR19973065.2479361/1‑150 (MQ=60)
                       ATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTT                                                                                                                                <  SRR19973065.786928/150‑1 (MQ=60)
                         AAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCC                                                                                                                              <  SRR19973065.2500834/150‑1 (MQ=60)
                          AAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCT                                                                                                                             >  SRR19973065.3359556/1‑150 (MQ=60)
                          AAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGCATTGAAGCACTCGATCTTCCT                                                                                                                             >  SRR19973065.1617861/1‑150 (MQ=60)
                           AAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTG                                                                                                                            <  SRR19973065.1294814/150‑1 (MQ=60)
                            AATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGA                                                                                                                           >  SRR19973065.1648327/1‑150 (MQ=60)
                            AATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGA                                                                                                                           >  SRR19973065.1648266/1‑150 (MQ=60)
                             ATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAA                                                                                                                          <  SRR19973065.1449849/150‑1 (MQ=60)
                               GTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGC                                                                                                                        >  SRR19973065.2863371/1‑150 (MQ=60)
                                   GCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCATATTTGGCGGGTTTTGCTTTTGGATGGAAGCACGCGATCTTCCTGAAGCTGAC                                                                                                                    <  SRR19973065.1111615/150‑1 (MQ=60)
                                   GCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGAC                                                                                                                    <  SRR19973065.1111648/150‑1 (MQ=60)
                                   GCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGAC                                                                                                                    <  SRR19973065.1967858/150‑1 (MQ=60)
                                   GCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGAC                                                                                                                    <  SRR19973065.1968819/150‑1 (MQ=60)
                                    CTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACG                                                                                                                   <  SRR19973065.3172925/150‑1 (MQ=60)
                                       ATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAG                                                                                                                >  SRR19973065.1285388/1‑150 (MQ=60)
                                          CCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGA                                                                                                             <  SRR19973065.600092/150‑1 (MQ=60)
                                          CCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGA                                                                                                             >  SRR19973065.1194803/1‑150 (MQ=60)
                                           CAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAG                                                                                                            >  SRR19973065.1902046/1‑150 (MQ=60)
                                           CAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAG                                                                                                            >  SRR19973065.3028539/1‑150 (MQ=60)
                                                 TCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCG                                                                                                      <  SRR19973065.1659792/150‑1 (MQ=60)
                                                 TCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCG                                                                                                      <  SRR19973065.1659798/150‑1 (MQ=60)
                                                    TCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAG                                                                                                   <  SRR19973065.621132/150‑1 (MQ=60)
                                                     CCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGT                                                                                                  >  SRR19973065.3345835/1‑150 (MQ=60)
                                                          ATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTT                                                                                             <  SRR19973065.3031976/150‑1 (MQ=60)
                                                          ATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTT                                                                                             >  SRR19973065.1959691/1‑150 (MQ=60)
                                                            TGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTA                                                                                           >  SRR19973065.2954451/1‑150 (MQ=60)
                                                            TGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTA                                                                                           >  SRR19973065.2954240/1‑150 (MQ=60)
                                                              TGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAG                                                                                         >  SRR19973065.2227647/1‑150 (MQ=60)
                                                              TGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAG                                                                                         >  SRR19973065.2227554/1‑150 (MQ=60)
                                                                GAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAA                                                                                       <  SRR19973065.3509649/150‑1 (MQ=60)
                                                                      CGGGATGATGGCCGGTGCTAATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCA                                                                                 <  SRR19973065.1809165/150‑1 (MQ=60)
                                                                                   GGTGCTCATGCTGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAATCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAA                                                                    <  SRR19973065.2468671/150‑1 (MQ=60)
                                                                                   GGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAA                                                                    <  SRR19973065.61287/150‑1 (MQ=60)
                                                                                   GGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAA                                                                    <  SRR19973065.61230/150‑1 (MQ=60)
                                                                                         CATGCGGCAAGCTGGTTTATCAATGGCGTCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAAT                                                              <  SRR19973065.247277/150‑1 (MQ=60)
                                                                                         CATGCGGCAAGCTGGTTTATCAATGGCGTCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAAT                                                              <  SRR19973065.247274/150‑1 (MQ=60)
                                                                                          ATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATG                                                             >  SRR19973065.3173695/1‑150 (MQ=60)
                                                                                             CGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGAT                                                          <  SRR19973065.2056585/150‑1 (MQ=60)
                                                                                             CGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGAT                                                          <  SRR19973065.2186422/150‑1 (MQ=60)
                                                                                               GCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAA                                                        <  SRR19973065.230514/150‑1 (MQ=60)
                                                                                                CAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAAATTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAAAATCAGGCGCAGGAGAAAAAGAATGTATAAT                                                       >  SRR19973065.2487794/1‑150 (MQ=60)
                                                                                                 AAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATC                                                      >  SRR19973065.2720378/1‑150 (MQ=60)
                                                                                                 AAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATC                                                      >  SRR19973065.2720386/1‑150 (MQ=60)
                                                                                                 AAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATC                                                      >  SRR19973065.2720458/1‑150 (MQ=60)
                                                                                                  AGCTGGTTGATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCG                                                     <  SRR19973065.2300588/150‑1 (MQ=60)
                                                                                                    CTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAG                                                   <  SRR19973065.2515767/150‑1 (MQ=60)
                                                                                                      GGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGC                                                 >  SRR19973065.3109293/1‑150 (MQ=60)
                                                                                                      GGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGC                                                 >  SRR19973065.3109332/1‑150 (MQ=60)
                                                                                                      GGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGC                                                 <  SRR19973065.3207670/150‑1 (MQ=60)
                                                                                                       GTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCG                                                <  SRR19973065.2554334/150‑1 (MQ=60)
                                                                                                         TTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAA                                              >  SRR19973065.117726/1‑150 (MQ=60)
                                                                                                           ATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATT                                            <  SRR19973065.2665039/150‑1 (MQ=60)
                                                                                                           ATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATT                                            >  SRR19973065.2597706/1‑150 (MQ=60)
                                                                                                              AATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTT                                         >  SRR19973065.981105/1‑150 (MQ=60)
                                                                                                               ATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTG                                        <  SRR19973065.835058/150‑1 (MQ=60)
                                                                                                               ATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTG                                        <  SRR19973065.3246652/150‑1 (MQ=60)
                                                                                                               ATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTG                                        <  SRR19973065.3246664/150‑1 (MQ=60)
                                                                                                                      CAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACG                                 <  SRR19973065.575500/150‑1 (MQ=60)
                                                                                                                      CAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACG                                 <  SRR19973065.163911/150‑1 (MQ=60)
                                                                                                                        AAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTT                               <  SRR19973065.1204851/150‑1 (MQ=60)
                                                                                                                         AACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTG                              <  SRR19973065.712721/150‑1 (MQ=60)
                                                                                                                         AACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTG                              <  SRR19973065.737928/150‑1 (MQ=60)
                                                                                                                           CACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCT                            <  SRR19973065.1669267/150‑1 (MQ=60)
                                                                                                                           CACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCT                            <  SRR19973065.1948456/150‑1 (MQ=60)
                                                                                                                             CCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCA                          <  SRR19973065.2423359/150‑1 (MQ=60)
                                                                                                                             CCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCA                          <  SRR19973065.836469/150‑1 (MQ=60)
                                                                                                                              CACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAG                         <  SRR19973065.2838500/150‑1 (MQ=60)
                                                                                                                              CACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAG                         <  SRR19973065.2798364/150‑1 (MQ=60)
                                                                                                                               ACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGG                        <  SRR19973065.213396/150‑1 (MQ=60)
                                                                                                                                CTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGC                       >  SRR19973065.1904249/1‑150 (MQ=60)
                                                                                                                                 TCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCA                      <  SRR19973065.1404296/150‑1 (MQ=60)
                                                                                                                                  CAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCAC                     >  SRR19973065.2854334/1‑150 (MQ=60)
                                                                                                                                     ATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGG                  <  SRR19973065.81827/150‑1 (MQ=60)
                                                                                                                                     ATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGG                  <  SRR19973065.81896/150‑1 (MQ=60)
                                                                                                                                      TTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGG                 <  SRR19973065.2967830/150‑1 (MQ=60)
                                                                                                                                             CGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCG          >  SRR19973065.375827/1‑150 (MQ=60)
                                                                                                                                                  AGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCG     <  SRR19973065.2168043/150‑1 (MQ=60)
                                                                                                                                                  AGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCG     <  SRR19973065.2167785/150‑1 (MQ=60)
                                                                                                                                                  AGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCG     <  SRR19973065.1975012/150‑1 (MQ=60)
                                                                                                                                                    CTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGAC   >  SRR19973065.1419338/1‑150 (MQ=60)
                                                                                                                                                     GACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  SRR19973065.638657/1‑150 (MQ=60)
                                                                                                                                                     GACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  SRR19973065.2108237/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  CP009273/319232‑319530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑