Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 319,381 G→T 100% D445Y (GAC→TAC)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273319,3810GT99.5% 661.2 / ‑4.2 199D445Y (GAC→TAC) ykgFferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (72/126);  total (72/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

AAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGC  >  CP009273/319236‑319523
                                                                                                                                                 |                                                                                                                                              
aaaaGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTAc                                                                                                                                              >  2:682661/1‑148 (MQ=255)
aaaaGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTAc                                                                                                                                              <  1:3690045/148‑1 (MQ=255)
   aGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACtgg                                                                                                                                           >  1:743922/1‑148 (MQ=255)
    gCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACtgga                                                                                                                                          <  1:301388/148‑1 (MQ=255)
           aaCGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGc                                                                                                                                   <  2:839066/148‑1 (MQ=255)
            aCGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCa                                                                                                                                  >  2:149564/1‑148 (MQ=255)
            aCGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCa                                                                                                                                  <  2:2242908/148‑1 (MQ=255)
              gggCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCAcg                                                                                                                                <  1:1207929/148‑1 (MQ=255)
                      aaaaTGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTc                                                                                                                        <  1:1100638/148‑1 (MQ=255)
                      aaaaTGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTc                                                                                                                        >  2:2187552/1‑148 (MQ=255)
                       aaaTGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTcc                                                                                                                       >  2:2545927/1‑148 (MQ=255)
                        aaTGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCt                                                                                                                      >  1:934651/1‑148 (MQ=255)
                          tGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGa                                                                                                                    <  1:3322562/148‑1 (MQ=255)
                          tGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGa                                                                                                                    >  2:1930736/1‑148 (MQ=255)
                          tGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGa                                                                                                                    <  1:3396126/148‑1 (MQ=255)
                          tGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGa                                                                                                                    <  2:3337564/148‑1 (MQ=255)
                                cTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGa                                                                                                              <  2:2128469/148‑1 (MQ=255)
                                 ttATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTCCTGGATGGAAGCACGCGATCTTCCTGAAGCTGAc                                                                                                             >  1:1337900/1‑148 (MQ=255)
                                 ttATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGAc                                                                                                             >  2:425774/1‑148 (MQ=255)
                                   aTGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACgg                                                                                                           >  1:3103118/1‑148 (MQ=255)
                                      ccAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGACCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgaga                                                                                                        <  2:3394730/148‑1 (MQ=255)
                                       cAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgagag                                                                                                       >  1:1461805/1‑148 (MQ=255)
                                        aaTAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGgagaga                                                                                                      >  1:3659531/1‑148 (MQ=255)
                                         aTAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGagagag                                                                                                     >  2:880367/1‑148 (MQ=255)
                                         aTAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGagagag                                                                                                     <  1:484324/148‑1 (MQ=255)
                                         aTAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGagagag                                                                                                     <  2:2733853/148‑1 (MQ=255)
                                          tAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGt                                                                                                    <  1:1589937/148‑1 (MQ=255)
                                              cATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTcc                                                                                                <  2:209740/148‑1 (MQ=255)
                                               aTCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCg                                                                                               >  2:3089987/1‑148 (MQ=255)
                                                 ccAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTa                                                                                             >  2:3456336/1‑148 (MQ=255)
                                                     gATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTg                                                                                         <  2:2445931/148‑1 (MQ=255)
                                                     gATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTg                                                                                         <  2:525208/148‑1 (MQ=255)
                                                        tgtgGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGtt                                                                                      <  2:1131949/148‑1 (MQ=255)
                                                          tgGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTa                                                                                    >  2:3171047/1‑148 (MQ=255)
                                                          tgGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTa                                                                                    >  2:2526331/1‑148 (MQ=255)
                                                             aaaGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGa                                                                                 <  1:2856478/148‑1 (MQ=255)
                                                             aaaGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGa                                                                                 >  2:3659169/1‑148 (MQ=255)
                                                               aGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGaaa                                                                               >  1:2174362/1‑148 (MQ=255)
                                                               aGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGaaa                                                                               <  2:2710325/148‑1 (MQ=255)
                                                                  cGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACAt                                                                            <  1:252034/148‑1 (MQ=255)
                                                                   gggATGATGGCCGGTGCTAATGCGGCAAGCTGGTTTATCAATGTCGGCAAAACAACACTCAAATTTGGCGCGATTAGCTACTGGATTGAAGCACGAGATCTTCCTGAAGCTTACGGAGAGAGTTTCCGTAGTTGGTTTAATAAACATc                                                                           <  2:571978/148‑1 (MQ=255)
                                                                    ggATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCa                                                                          <  1:533056/148‑1 (MQ=255)
                                                                    ggATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCa                                                                          <  1:2301340/148‑1 (MQ=255)
                                                                      atgatgGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAgg                                                                        <  1:475855/148‑1 (MQ=255)
                                                                      atgatgGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAgg                                                                        <  1:360339/148‑1 (MQ=255)
                                                                         atgGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGgcgc                                                                     <  2:1086369/148‑1 (MQ=255)
                                                                         atgGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGgcgc                                                                     <  2:1086402/148‑1 (MQ=255)
                                                                          tgGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCa                                                                    <  1:2145051/148‑1 (MQ=255)
                                                                           ggCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAg                                                                   <  1:2345420/148‑1 (MQ=255)
                                                                            gCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAgg                                                                  <  2:2056931/148‑1 (MQ=255)
                                                                             ccGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGga                                                                 <  1:2541796/148‑1 (MQ=255)
                                                                                  gCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGaaaa                                                            <  1:875282/148‑1 (MQ=255)
                                                                                  gCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGaaaa                                                            <  2:853548/148‑1 (MQ=255)
                                                                                    tCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAg                                                          <  1:2652038/148‑1 (MQ=255)
                                                                                     cATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGa                                                         <  2:743922/148‑1 (MQ=255)
                                                                                     cATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGa                                                         <  2:934681/148‑1 (MQ=255)
                                                                                     cATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGa                                                         <  1:1930736/148‑1 (MQ=255)
                                                                                     cATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGa                                                         <  1:79469/148‑1 (MQ=255)
                                                                                       tGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAAt                                                       <  2:1349299/148‑1 (MQ=255)
                                                                                       tGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAAt                                                       <  2:1023930/148‑1 (MQ=255)
                                                                                        gCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATg                                                      <  2:2038098/148‑1 (MQ=255)
                                                                                         cGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATgg                                                     <  1:834637/148‑1 (MQ=255)
                                                                                          ggCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGa                                                    <  1:3174922/148‑1 (MQ=255)
                                                                                             aaGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATaa                                                 <  1:254069/148‑1 (MQ=255)
                                                                                             aaGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATaa                                                 <  2:1462176/148‑1 (MQ=255)
                                                                                                cTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCg                                              <  1:363812/148‑1 (MQ=255)
                                                                                                cTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCg                                              <  1:1003523/148‑1 (MQ=255)
                                                                                                cTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCg                                              <  1:2503665/148‑1 (MQ=255)
                                                                                                  ggTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAg                                            >  2:854347/1‑148 (MQ=255)
                                                                                                  ggTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAg                                            <  2:3586367/148‑1 (MQ=255)
                                                                                                      tATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGa                                        <  1:2711629/148‑1 (MQ=255)
                                                                                                      tATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGa                                        >  1:3675884/1‑148 (MQ=255)
                                                                                                       aTCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGaa                                       <  2:1784123/148‑1 (MQ=255)
                                                                                                       aTCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGaa                                       <  2:3674715/148‑1 (MQ=255)
                                                                                                       aTCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGaa                                       <  2:3659531/148‑1 (MQ=255)
                                                                                                       aTCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGaa                                       >  2:1030631/1‑148 (MQ=255)
                                                                                                         cAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAtt                                     >  1:2811742/1‑148 (MQ=255)
                                                                                                          aaTGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAttt                                    <  1:677672/148‑1 (MQ=255)
                                                                                                           aTGGCGGCAAAACACCACTCAAATTTGGTGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAtttt                                   >  1:1724930/1‑148 (MQ=255)
                                                                                                           aTGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAtttt                                   >  1:1794309/1‑148 (MQ=255)
                                                                                                           aTGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAtttt                                   <  1:2882171/148‑1 (MQ=255)
                                                                                                               cggcAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGaa                               <  2:1842973/148‑1 (MQ=255)
                                                                                                               cggcAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGaa                               <  2:3732453/148‑1 (MQ=255)
                                                                                                               cggcAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGaa                               <  2:2601466/148‑1 (MQ=255)
                                                                                                                 gcAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATa                             <  2:3143532/148‑1 (MQ=255)
                                                                                                                   aaaaCACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAAc                           <  2:1356488/148‑1 (MQ=255)
                                                                                                                    aaaCACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACg                          <  2:2357577/148‑1 (MQ=255)
                                                                                                                     cgcaccacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGt                         <  1:2016850/146‑1 (MQ=255)
                                                                                                                     aaCACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGt                         <  2:3015851/148‑1 (MQ=255)
                                                                                                                     aaCACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGt                         >  1:564676/1‑148 (MQ=255)
                                                                                                                      acacCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGtt                        >  1:1054693/1‑148 (MQ=255)
                                                                                                                        accacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGc                      >  2:1688280/1‑148 (MQ=255)
                                                                                                                         ccacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCt                     <  2:589632/148‑1 (MQ=255)
                                                                                                                         ccacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCt                     <  1:1527996/148‑1 (MQ=255)
                                                                                                                          cacTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTc                    <  2:2582689/148‑1 (MQ=255)
                                                                                                                            cTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAg                  >  2:1654474/1‑148 (MQ=255)
                                                                                                                                       cgcgATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCg       >  2:279030/1‑148 (MQ=255)
                                                                                                                                           aTTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCg   >  1:3503936/1‑148 (MQ=255)
                                                                                                                                           aTTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCg   <  2:2519540/148‑1 (MQ=255)
                                                                                                                                            ttAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGc  >  2:1922269/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
AAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGC  >  CP009273/319236‑319523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  CP009273/319232‑319530
                                                                                                                                                     |                                                                                                                                                     
CCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCT                                                                                                                                                       <  SRR19973069.2244893/150‑1 (MQ=60)
 CGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTA                                                                                                                                                      <  SRR19973069.1606016/150‑1 (MQ=60)
 CGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTA                                                                                                                                                      >  SRR19973069.3145452/1‑150 (MQ=60)
 CGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTA                                                                                                                                                      <  SRR19973069.1504013/150‑1 (MQ=60)
  GCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTAC                                                                                                                                                     <  SRR19973069.3199676/150‑1 (MQ=60)
    AAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTG                                                                                                                                                   <  SRR19973069.3714149/150‑1 (MQ=60)
    AAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTG                                                                                                                                                   >  SRR19973069.2538199/1‑150 (MQ=60)
    AAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTG                                                                                                                                                   <  SRR19973069.3714178/150‑1 (MQ=60)
     AAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGG                                                                                                                                                  >  SRR19973069.749188/1‑150 (MQ=60)
      AAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGA                                                                                                                                                 >  SRR19973069.3698755/1‑150 (MQ=60)
      AAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGA                                                                                                                                                 >  SRR19973069.815202/1‑150 (MQ=60)
      AAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGA                                                                                                                                                 >  SRR19973069.1796689/1‑150 (MQ=60)
        GCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATG                                                                                                                                               <  SRR19973069.303431/150‑1 (MQ=60)
        GCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATG                                                                                                                                               <  SRR19973069.2120622/150‑1 (MQ=60)
                  GGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCG                                                                                                                                     <  SRR19973069.1216225/150‑1 (MQ=60)
                       ATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTT                                                                                                                                >  SRR19973069.753020/1‑150 (MQ=60)
                       ATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTT                                                                                                                                <  SRR19973069.1109199/150‑1 (MQ=60)
                        TAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTC                                                                                                                               >  SRR19973069.1899079/1‑150 (MQ=60)
                          AAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCT                                                                                                                             >  SRR19973069.940988/1‑150 (MQ=60)
                          AAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCT                                                                                                                             >  SRR19973069.941018/1‑150 (MQ=60)
                          AAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCT                                                                                                                             <  SRR19973069.2850231/150‑1 (MQ=60)
                          AAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCT                                                                                                                             <  SRR19973069.1108330/150‑1 (MQ=60)
                              TGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAG                                                                                                                         <  SRR19973069.3418294/150‑1 (MQ=60)
                              TGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAG                                                                                                                         <  SRR19973069.3418252/150‑1 (MQ=60)
                              TGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAG                                                                                                                         <  SRR19973069.3344348/150‑1 (MQ=60)
                                   GCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTCCTGGATGGAAGCACGCGATCTTCCTGAAGCTGAC                                                                                                                    >  SRR19973069.1347135/1‑150 (MQ=60)
                                   GCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGAC                                                                                                                    >  SRR19973069.3035858/1‑150 (MQ=60)
                                     TTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGG                                                                                                                  >  SRR19973069.3123545/1‑150 (MQ=60)
                                       ATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAG                                                                                                                >  SRR19973069.2536295/1‑150 (MQ=60)
                                         GCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAG                                                                                                              <  SRR19973069.855558/150‑1 (MQ=60)
                                         GCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAG                                                                                                              >  SRR19973069.1472008/1‑150 (MQ=60)
                                          CCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGA                                                                                                             >  SRR19973069.3683490/1‑150 (MQ=60)
                                          CCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGA                                                                                                             >  SRR19973069.3682510/1‑150 (MQ=60)
                                           CAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAG                                                                                                            >  SRR19973069.3164100/1‑150 (MQ=60)
                                           CAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAG                                                                                                            >  SRR19973069.2327893/1‑150 (MQ=60)
                                            AATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGT                                                                                                           <  SRR19973069.1377715/150‑1 (MQ=60)
                                             ATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTT                                                                                                          <  SRR19973069.487832/150‑1 (MQ=60)
                                             ATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTT                                                                                                          <  SRR19973069.487907/150‑1 (MQ=60)
                                              TAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTT                                                                                                         <  SRR19973069.252096/150‑1 (MQ=60)
                                              TAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTT                                                                                                         <  SRR19973069.1600090/150‑1 (MQ=60)
                                              TAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTT                                                                                                         <  SRR19973069.1600695/150‑1 (MQ=60)
                                                              TGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAG                                                                                         <  SRR19973069.2923164/150‑1 (MQ=60)
                                                                 AAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAA                                                                                      >  SRR19973069.2189205/1‑150 (MQ=60)
                                                                 AAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAA                                                                                      <  SRR19973069.2875271/150‑1 (MQ=60)
                                                                      CGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCA                                                                                 >  SRR19973069.1237785/1‑150 (MQ=60)
                                                                      CGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCA                                                                                 <  SRR19973069.253879/150‑1 (MQ=60)
                                                                       GGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAG                                                                                >  SRR19973069.3259713/1‑150 (MQ=60)
                                                                        GGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGG                                                                               <  SRR19973069.536815/150‑1 (MQ=60)
                                                                        GGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGG                                                                               <  SRR19973069.1106467/150‑1 (MQ=60)
                                                                        GGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGG                                                                               <  SRR19973069.2316988/150‑1 (MQ=60)
                                                                          ATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCG                                                                             <  SRR19973069.362933/150‑1 (MQ=60)
                                                                          ATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCG                                                                             <  SRR19973069.479470/150‑1 (MQ=60)
                                                                          ATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCG                                                                             <  SRR19973069.479314/150‑1 (MQ=60)
                                                                              TGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGG                                                                         <  SRR19973069.2159770/150‑1 (MQ=60)
                                                                               GGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGA                                                                        <  SRR19973069.2361369/150‑1 (MQ=60)
                                                                                 CCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGA                                                                      <  SRR19973069.2558613/150‑1 (MQ=60)
                                                                                 CCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGA                                                                      <  SRR19973069.2558158/150‑1 (MQ=60)
                                                                                      GCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAG                                                                 <  SRR19973069.2708926/150‑1 (MQ=60)
                                                                                      GCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAG                                                                 <  SRR19973069.881366/150‑1 (MQ=60)
                                                                                        TCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAA                                                               <  SRR19973069.2669802/150‑1 (MQ=60)
                                                                                         CATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAAT                                                              <  SRR19973069.434337/150‑1 (MQ=60)
                                                                                         CATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAAT                                                              <  SRR19973069.80335/150‑1 (MQ=60)
                                                                                         CATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAAT                                                              <  SRR19973069.1943987/150‑1 (MQ=60)
                                                                                         CATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAAT                                                              <  SRR19973069.3471149/150‑1 (MQ=60)
                                                                                            GCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGA                                                           <  SRR19973069.935507/150‑1 (MQ=60)
                                                                                             CGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGAT                                                          <  SRR19973069.840513/150‑1 (MQ=60)
                                                                                             CGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGAT                                                          <  SRR19973069.60861/150‑1 (MQ=60)
                                                                                              GGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATA                                                         <  SRR19973069.3587695/150‑1 (MQ=60)
                                                                                              GGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATA                                                         <  SRR19973069.3195585/150‑1 (MQ=60)
                                                                                                CAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAAT                                                       <  SRR19973069.1974406/150‑1 (MQ=60)
                                                                                                 AAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATC                                                      <  SRR19973069.255922/150‑1 (MQ=60)
                                                                                                 AAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATC                                                      <  SRR19973069.687408/150‑1 (MQ=60)
                                                                                                    CTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAG                                                   <  SRR19973069.2520374/150‑1 (MQ=60)
                                                                                                    CTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAG                                                   <  SRR19973069.1010413/150‑1 (MQ=60)
                                                                                                    CTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAG                                                   <  SRR19973069.366440/150‑1 (MQ=60)
                                                                                                    CTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAG                                                   <  SRR19973069.365866/150‑1 (MQ=60)
                                                                                                        TTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGA                                               >  SRR19973069.3699931/1‑150 (MQ=60)
                                                                                                          TATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAT                                             <  SRR19973069.2729811/150‑1 (MQ=60)
                                                                                                           ATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATT                                            >  SRR19973069.2830371/1‑150 (MQ=60)
                                                                                                             CAATGGCGGCAAAACACCACTCAAATTTGGTGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGACAAAGAATGGATAATCGAGGCGAATTTT                                          >  SRR19973069.1736216/1‑150 (MQ=60)
                                                                                                             CAATGGCGGCAAAACACCACTCAAATTTGGTGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTT                                          >  SRR19973069.1737016/1‑150 (MQ=60)
                                                                                                             CAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTT                                          >  SRR19973069.1806945/1‑150 (MQ=60)
                                                                                                              AATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTT                                         <  SRR19973069.682370/150‑1 (MQ=60)
                                                                                                               ATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTG                                        <  SRR19973069.2166584/150‑1 (MQ=60)
                                                                                                               ATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTG                                        <  SRR19973069.2901110/150‑1 (MQ=60)
                                                                                                                   CGGCAAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATA                                    <  SRR19973069.2900154/150‑1 (MQ=60)
                                                                                                                      AAAAACACCACTCAAATTTGGCGCGTTTAGCTACTGGATGGAAGCAGGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACG                                 <  SRR19973069.2202447/150‑1 (MQ=60)
                                                                                                                       AAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGT                                >  SRR19973069.568570/1‑150 (MQ=60)
                                                                                                                       AAAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGT                                >  SRR19973069.568605/1‑150 (MQ=60)
                                                                                                                        AAACACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTT                               >  SRR19973069.1062024/1‑150 (MQ=60)
                                                                                                                         cgCACCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTG                              <  SRR19973069.2030877/148‑1 (MQ=60)
                                                                                                                             CCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCA                          <  SRR19973069.1538499/150‑1 (MQ=60)
                                                                                                                             CCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCA                          <  SRR19973069.1538505/150‑1 (MQ=60)
                                                                                                                             CCACTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCA                          >  SRR19973069.278690/1‑150 (MQ=60)
                                                                                                                                CTCAAATTTGGCGCGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGG                       <  SRR19973069.2562757/150‑1 (MQ=60)
                                                                                                                                             CGATTAGCTACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCG          >  SRR19973069.3526492/1‑150 (MQ=60)
                                                                                                                                                     TACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  SRR19973069.3523113/1‑150 (MQ=60)
                                                                                                                                                     TACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  SRR19973069.1614268/1‑150 (MQ=60)
                                                                                                                                                     TACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  SRR19973069.2195768/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACT  >  CP009273/319232‑319530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑