Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,104,639 C→A 100% intergenic (‑242/‑152) opgC ← / → opgG osmoregulated periplasmic glucan succinylation membrane protein/osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,104,6390CA98.8% 805.8 / 0.4 254intergenic (‑242/‑152)opgC/opgGosmoregulated periplasmic glucan succinylation membrane protein/osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (121/130);  total (123/131)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

CAGGCTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAA  >  CP009273/1104494‑1104783
                                                                                                                                                 |                                                                                                                                                
cAGGCTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAgg                                                                                                                                                >  2:208864/1‑148 (MQ=255)
 aGGCTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGt                                                                                                                                               <  2:1715449/148‑1 (MQ=255)
    cTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGc                                                                                                                                            >  2:1875000/1‑148 (MQ=255)
     ttCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCa                                                                                                                                           <  2:2425668/148‑1 (MQ=255)
       cAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAAt                                                                                                                                         >  1:1127669/1‑148 (MQ=255)
       cAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAAt                                                                                                                                         <  2:470919/148‑1 (MQ=255)
        aaGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATc                                                                                                                                        <  1:3420132/148‑1 (MQ=255)
         aGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCa                                                                                                                                       <  2:802633/148‑1 (MQ=255)
             ttttATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTg                                                                                                                                   <  1:2184833/148‑1 (MQ=255)
             ttttATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTg                                                                                                                                   <  1:2022261/148‑1 (MQ=255)
                tATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGACAGTGTCAAAGGTTGCAATCAGGTGCtt                                                                                                                                >  1:2349255/1‑148 (MQ=255)
                tATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCtt                                                                                                                                >  1:2869724/1‑148 (MQ=255)
                   gCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAAt                                                                                                                             <  2:1885296/148‑1 (MQ=255)
                    cATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATc                                                                                                                            <  2:1311963/148‑1 (MQ=255)
                    cATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATc                                                                                                                            >  2:3281418/1‑148 (MQ=255)
                     aTAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATcc                                                                                                                           <  1:1676574/148‑1 (MQ=255)
                      tAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCa                                                                                                                          >  2:3105578/1‑148 (MQ=255)
                      tAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCa                                                                                                                          >  2:1727640/1‑148 (MQ=255)
                       aGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCAt                                                                                                                         >  1:2620474/1‑148 (MQ=255)
                        gCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATg                                                                                                                        >  1:71484/1‑148 (MQ=255)
                          atcatcGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAg                                                                                                                      <  2:275911/148‑1 (MQ=255)
                           tcatcGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGc                                                                                                                     >  2:1209142/1‑148 (MQ=255)
                           tcatcGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGc                                                                                                                     <  1:765561/148‑1 (MQ=255)
                            catcGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGcc                                                                                                                    >  2:2583140/1‑148 (MQ=255)
                               cGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGc                                                                                                                 >  2:51885/1‑148 (MQ=255)
                               cGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGc                                                                                                                 <  2:778402/148‑1 (MQ=255)
                               cGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGc                                                                                                                 >  1:1314732/1‑148 (MQ=255)
                               cGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGc                                                                                                                 >  1:2316225/1‑148 (MQ=255)
                                gCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCg                                                                                                                <  2:2358996/148‑1 (MQ=255)
                                     cACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTg                                                                                                           >  1:699714/1‑148 (MQ=255)
                                       cTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGaa                                                                                                         >  1:2645677/1‑148 (MQ=255)
                                       cTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGaa                                                                                                         >  1:2477355/1‑148 (MQ=255)
                                         aaCCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACg                                                                                                       <  2:3507046/148‑1 (MQ=255)
                                          aCCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGa                                                                                                      >  1:400101/1‑148 (MQ=255)
                                           ccAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGAt                                                                                                     <  2:2198256/148‑1 (MQ=255)
                                               aaTGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCg                                                                                                 >  1:313373/1‑148 (MQ=255)
                                                    aaGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGAtt                                                                                            >  1:3381371/1‑148 (MQ=255)
                                                       cGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCtg                                                                                         <  2:2922293/148‑1 (MQ=255)
                                                       cGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCtg                                                                                         >  1:3314456/1‑148 (MQ=255)
                                                        gTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCtgt                                                                                        <  1:3233433/148‑1 (MQ=255)
                                                          cTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCtgttg                                                                                      >  2:473962/1‑148 (MQ=255)
                                                            gTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTc                                                                                    >  2:1891503/1‑148 (MQ=255)
                                                               aGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGa                                                                                 <  2:1999261/148‑1 (MQ=255)
                                                               aGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGa                                                                                 <  2:2711430/148‑1 (MQ=255)
                                                               aGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGa                                                                                 <  2:3145685/148‑1 (MQ=255)
                                                                gACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGaa                                                                                <  2:1268506/148‑1 (MQ=255)
                                                                  cGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATg                                                                              <  1:1918463/148‑1 (MQ=255)
                                                                   ggTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATgg                                                                             >  1:894111/1‑148 (MQ=255)
                                                                     ttGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCt                                                                           >  2:2071936/1‑148 (MQ=255)
                                                                     ttGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCt                                                                           >  2:742549/1‑148 (MQ=255)
                                                                     ttGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCt                                                                           >  2:2502007/1‑148 (MQ=255)
                                                                            ataaatTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATc                                                                    <  2:2959758/148‑1 (MQ=255)
                                                                                atTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCAtt                                                                >  1:379866/1‑148 (MQ=255)
                                                                                 tttGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTa                                                               >  2:1199635/1‑148 (MQ=255)
                                                                                 tttGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTa                                                               <  1:1192145/148‑1 (MQ=255)
                                                                                      tGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATa                                                          <  1:3001509/148‑1 (MQ=255)
                                                                                       ggCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAg                                                         <  1:1429989/148‑1 (MQ=255)
                                                                                       ggCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAg                                                         <  1:976154/148‑1 (MQ=255)
                                                                                       ggCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAg                                                         <  1:2730996/148‑1 (MQ=255)
                                                                                       ggCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAg                                                         <  1:2885790/148‑1 (MQ=255)
                                                                                            aCCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAgatcgg                                                    >  1:1116858/1‑148 (MQ=255)
                                                                                            aCCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAgatcgg                                                    >  2:2237898/1‑148 (MQ=255)
                                                                                                 acacGAAGTCGATGCTTCTGTCTTCAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGATCGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGa                                               >  1:1605665/1‑148 (MQ=255)
                                                                                                  cacGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGat                                              >  2:1534964/1‑148 (MQ=255)
                                                                                                  cacGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGat                                              <  2:71363/148‑1 (MQ=255)
                                                                                                  cacGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGat                                              <  2:725160/148‑1 (MQ=255)
                                                                                                      aaGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATaa                                          <  2:379849/148‑1 (MQ=255)
                                                                                                      aaGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATaa                                          <  2:964386/148‑1 (MQ=255)
                                                                                                       aGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAg                                         <  1:2511852/148‑1 (MQ=255)
                                                                                                       aGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAg                                         >  2:490403/1‑148 (MQ=255)
                                                                                                        gTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGc                                        <  1:1199635/148‑1 (MQ=255)
                                                                                                        gTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGc                                        >  1:52388/1‑148 (MQ=255)
                                                                                                               cTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACaaa                                 <  2:2437306/148‑1 (MQ=255)
                                                                                                               cTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACaaa                                 <  2:676701/148‑1 (MQ=255)
                                                                                                                ttCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAg                                <  1:330470/148‑1 (MQ=255)
                                                                                                                 tCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAgg                               >  2:2080827/1‑148 (MQ=255)
                                                                                                                   tGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAgggg                             <  2:1122431/148‑1 (MQ=255)
                                                                                                                    gTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAggggg                            >  1:631642/1‑148 (MQ=255)
                                                                                                                    gTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAggggg                            >  2:3075064/1‑148 (MQ=255)
                                                                                                                     tCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTTCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTTTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGa                           <  1:461159/148‑1 (MQ=255)
                                                                                                                      cTTTAGGAGAAGCACGGAAAGTGAAAAAGTTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGaa                          <  2:3504465/148‑1 (MQ=255)
                                                                                                                      cTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGaa                          >  1:1426869/1‑148 (MQ=255)
                                                                                                                      cTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGaa                          >  1:1694675/1‑148 (MQ=255)
                                                                                                                          aGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGc                      <  2:2869724/148‑1 (MQ=255)
                                                                                                                          aGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGc                      <  1:1367449/148‑1 (MQ=255)
                                                                                                                            gagaAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCtt                    <  2:1628255/148‑1 (MQ=255)
                                                                                                                              gaAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTAc                  <  2:3368914/148‑1 (MQ=255)
                                                                                                                              gaAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTAc                  >  1:1863181/1‑148 (MQ=255)
                                                                                                                                aGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCAAGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGAACACAAAGGGGGAAGTGCTTACTa                >  1:2500576/1‑148 (MQ=255)
                                                                                                                                 gCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTaa               >  1:466224/1‑148 (MQ=255)
                                                                                                                                  cACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAAt              >  2:2996461/1‑148 (MQ=255)
                                                                                                                                  cACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAAt              <  1:316268/148‑1 (MQ=255)
                                                                                                                                   aCGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAAtt             <  2:403276/148‑1 (MQ=255)
                                                                                                                                       aaaGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGa         >  1:1994604/1‑148 (MQ=255)
                                                                                                                                           tGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACa     >  1:1962020/1‑148 (MQ=255)
                                                                                                                                            gAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACAt    <  2:2689490/148‑1 (MQ=255)
                                                                                                                                            gAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACAt    <  1:3021680/148‑1 (MQ=255)
                                                                                                                                             aaaaAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATa   <  1:2573675/148‑1 (MQ=255)
                                                                                                                                              aaaaGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATaa  >  2:592124/1‑148 (MQ=255)
                                                                                                                                              aaaaGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATaa  <  1:208864/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAGGCTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAA  >  CP009273/1104494‑1104783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAGGCTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTAC  >  CP009273/1104492‑1104788
                                                                                                                                                   |                                                                                                                                                     
CGCAGGCTTCAAGTTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGG                                                                                                                                                     <  SRR19973065.686098/150‑1 (MQ=60)
CGCAGGCTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGG                                                                                                                                                     <  SRR19973065.2057260/150‑1 (MQ=60)
 GCAGGCTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGT                                                                                                                                                    <  SRR19973065.3130022/150‑1 (MQ=60)
      CTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAA                                                                                                                                               >  SRR19973065.1129743/1‑150 (MQ=60)
       TTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAAT                                                                                                                                              >  SRR19973065.2895744/1‑150 (MQ=60)
       TTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAAT                                                                                                                                              <  SRR19973065.2534241/150‑1 (MQ=60)
       TTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAAT                                                                                                                                              >  SRR19973065.1135004/1‑150 (MQ=60)
        TCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATC                                                                                                                                             <  SRR19973065.2356845/150‑1 (MQ=60)
         CAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCA                                                                                                                                            <  SRR19973065.887472/150‑1 (MQ=60)
          AAGGTTTTTATTCATAGCATCATCGCTAACACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAG                                                                                                                                           <  SRR19973065.1959519/150‑1 (MQ=60)
          AAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAG                                                                                                                                           <  SRR19973065.3441027/150‑1 (MQ=60)
          AAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAG                                                                                                                                           >  SRR19973065.3454174/1‑150 (MQ=60)
          AAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAG                                                                                                                                           <  SRR19973065.1692607/150‑1 (MQ=60)
             GTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTG                                                                                                                                        >  SRR19973065.730019/1‑150 (MQ=60)
               TTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCT                                                                                                                                      <  SRR19973065.2198850/150‑1 (MQ=60)
               TTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCT                                                                                                                                      <  SRR19973065.2036087/150‑1 (MQ=60)
               TTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCT                                                                                                                                      <  SRR19973065.2035268/150‑1 (MQ=60)
                TTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGACAGTGTCAAAGGTTGCAATCAGGTGCTT                                                                                                                                     >  SRR19973065.2363891/1‑150 (MQ=60)
                TTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTT                                                                                                                                     >  SRR19973065.2887322/1‑150 (MQ=60)
                 TTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTA                                                                                                                                    >  SRR19973065.2513456/1‑150 (MQ=60)
                     GCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCC                                                                                                                                <  SRR19973065.148761/150‑1 (MQ=60)
                       ATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCAT                                                                                                                              <  SRR19973065.1687788/150‑1 (MQ=60)
                       ATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCAT                                                                                                                              >  SRR19973065.2636695/1‑150 (MQ=60)
                       ATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCAT                                                                                                                              >  SRR19973065.2636801/1‑150 (MQ=60)
                        TAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATG                                                                                                                             >  SRR19973065.72231/1‑150 (MQ=60)
                        TAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATG                                                                                                                             <  SRR19973065.2305726/150‑1 (MQ=60)
                        TAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATG                                                                                                                             >  SRR19973065.72202/1‑150 (MQ=60)
                        TAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATG                                                                                                                             >  SRR19973065.72109/1‑150 (MQ=60)
                          GCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAG                                                                                                                           <  SRR19973065.1149759/150‑1 (MQ=60)
                             TCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCA                                                                                                                        <  SRR19973065.770650/150‑1 (MQ=60)
                              CATCGCTACCACTAACCAGAATGGAATCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAG                                                                                                                       >  SRR19973065.3153224/1‑150 (MQ=60)
                               ATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGC                                                                                                                      >  SRR19973065.2330750/1‑150 (MQ=60)
                               ATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGC                                                                                                                      >  SRR19973065.1323425/1‑150 (MQ=60)
                                    TACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCT                                                                                                                 <  SRR19973065.3026870/150‑1 (MQ=60)
                                    TACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCT                                                                                                                 <  SRR19973065.1269435/150‑1 (MQ=60)
                                     ACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTG                                                                                                                >  SRR19973065.704373/1‑150 (MQ=60)
                                       CACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAA                                                                                                              >  SRR19973065.2661990/1‑150 (MQ=60)
                                       CACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAA                                                                                                              >  SRR19973065.1190744/1‑150 (MQ=60)
                                       CACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAA                                                                                                              >  SRR19973065.2636580/1‑150 (MQ=60)
                                       CACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAA                                                                                                              >  SRR19973065.2492953/1‑150 (MQ=60)
                                        ACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAAC                                                                                                             <  SRR19973065.1203067/150‑1 (MQ=60)
                                         CTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACG                                                                                                            <  SRR19973065.3281362/150‑1 (MQ=60)
                                          TAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGA                                                                                                           >  SRR19973065.402901/1‑150 (MQ=60)
                                          TAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGA                                                                                                           >  SRR19973065.402984/1‑150 (MQ=60)
                                            ACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATA                                                                                                         >  SRR19973065.454151/1‑150 (MQ=60)
                                               AGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCG                                                                                                      >  SRR19973065.2749712/1‑150 (MQ=60)
                                               AGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCG                                                                                                      >  SRR19973065.315490/1‑150 (MQ=60)
                                                 AATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGG                                                                                                    <  SRR19973065.1524516/150‑1 (MQ=60)
                                                    GGAAGCGTCTGTAAGACGGTTTATAAATAAATTTGCTGGCAAACCCTCCACGACGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAACAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATT                                                                                                 <  SRR19973065.3424419/150‑1 (MQ=60)
                                                    GGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATT                                                                                                 >  SRR19973065.3402047/1‑150 (MQ=60)
                                                    GGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATT                                                                                                 >  SRR19973065.2730098/1‑150 (MQ=60)
                                                       AGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTG                                                                                              >  SRR19973065.3334665/1‑150 (MQ=60)
                                                          GTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTG                                                                                           <  SRR19973065.3252538/150‑1 (MQ=60)
                                                          GTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTG                                                                                           <  SRR19973065.3252876/150‑1 (MQ=60)
                                                          GTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTG                                                                                           >  SRR19973065.1623710/1‑150 (MQ=60)
                                                          GTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTG                                                                                           <  SRR19973065.3252550/150‑1 (MQ=60)
                                                             TGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCG                                                                                        >  SRR19973065.2778181/1‑150 (MQ=60)
                                                              GTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACAAGAATTCTATGCTTCTGTCTTTAGGAGAAGCACGTAACTTGAAAAATGTTTCAATCCGGTGCTTAATCCATGATCCAGCGTGCTGAACGATACCGGGATTATGTTGTCGT                                                                                       >  SRR19973065.3379112/1‑150 (MQ=60)
                                                               TAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGA                                                                                      >  SRR19973065.2483656/1‑150 (MQ=60)
                                                                  GACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATG                                                                                   <  SRR19973065.1216065/150‑1 (MQ=60)
                                                                   ACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGG                                                                                  >  SRR19973065.899791/1‑150 (MQ=60)
                                                                    CGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGC                                                                                 <  SRR19973065.1931046/150‑1 (MQ=60)
                                                                    CGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGC                                                                                 >  SRR19973065.2405441/1‑150 (MQ=60)
                                                                      GTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTG                                                                               >  SRR19973065.3326476/1‑150 (MQ=60)
                                                                            AAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATC                                                                         >  SRR19973065.1832913/1‑150 (MQ=60)
                                                                                AAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATT                                                                     >  SRR19973065.382509/1‑150 (MQ=60)
                                                                                AAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATT                                                                     >  SRR19973065.382526/1‑150 (MQ=60)
                                                                                   TTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAA                                                                  <  SRR19973065.1199710/150‑1 (MQ=60)
                                                                                        TGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGA                                                             <  SRR19973065.3299419/150‑1 (MQ=60)
                                                                                        TGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGA                                                             <  SRR19973065.3019898/150‑1 (MQ=60)
                                                                                         GGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGAT                                                            <  SRR19973065.2903439/150‑1 (MQ=60)
                                                                                         GGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGAT                                                            <  SRR19973065.982638/150‑1 (MQ=60)
                                                                                         GGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGAT                                                            <  SRR19973065.1439290/150‑1 (MQ=60)
                                                                                         GGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGAT                                                            <  SRR19973065.2747987/150‑1 (MQ=60)
                                                                                         GGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGAT                                                            <  SRR19973065.1439122/150‑1 (MQ=60)
                                                                                            AAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGG                                                         >  SRR19973065.1375939/1‑150 (MQ=60)
                                                                                            AAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGG                                                         >  SRR19973065.1124150/1‑150 (MQ=60)
                                                                                            AAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGG                                                         >  SRR19973065.1409462/1‑150 (MQ=60)
                                                                                             AACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGA                                                        <  SRR19973065.3106705/150‑1 (MQ=60)
                                                                                             AACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGA                                                        >  SRR19973065.387845/1‑150 (MQ=60)
                                                                                               CCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATAAAAATAGATCGGATC                                                      <  SRR19973065.1653024/150‑1 (MQ=60)
                                                                                                 CTACACGAAGTCGATGCTTCTGTCTTCAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGATCGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGA                                                    >  SRR19973065.1616409/1‑150 (MQ=60)
                                                                                                    CACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATAT                                                 >  SRR19973065.2251244/1‑150 (MQ=60)
                                                                                                      CGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAA                                               <  SRR19973065.953729/150‑1 (MQ=60)
                                                                                                        AAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGC                                             >  SRR19973065.52966/1‑150 (MQ=60)
                                                                                                        AAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGC                                             >  SRR19973065.52771/1‑150 (MQ=60)
                                                                                                         AGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCA                                            <  SRR19973065.2527634/150‑1 (MQ=60)
                                                                                                         AGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCA                                            <  SRR19973065.2527541/150‑1 (MQ=60)
                                                                                                          GTCGATTCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCAC                                           <  SRR19973065.1207298/150‑1 (MQ=60)
                                                                                                                  TTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGG                                   <  SRR19973065.332783/150‑1 (MQ=60)
                                                                                                                  TTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGG                                   <  SRR19973065.2107123/150‑1 (MQ=60)
                                                                                                                    CTGTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGG                                 >  SRR19973065.635752/1‑150 (MQ=60)
                                                                                                                      GTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAA                               >  SRR19973065.1705988/1‑150 (MQ=60)
                                                                                                                      GTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAA                               >  SRR19973065.1436161/1‑150 (MQ=60)
                                                                                                                      GTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAA                               >  SRR19973065.1436150/1‑150 (MQ=60)
                                                                                                                      GTCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAA‑GGGGAAG                              <  SRR19973065.1912335/150‑1 (MQ=60)
                                                                                                                       TCTTTAGGAGAAGCACGGAAAGTGAAAAAGGTTTCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTTTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAG                              <  SRR19973065.464339/150‑1 (MQ=60)
                                                                                                                           TAGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCT                          >  SRR19973065.2642221/1‑150 (MQ=60)
                                                                                                                            AGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTT                         <  SRR19973065.1376485/150‑1 (MQ=60)
                                                                                                                            AGGAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTT                         <  SRR19973065.3130702/150‑1 (MQ=60)
                                                                                                                              GAGAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTAC                       >  SRR19973065.1875443/1‑150 (MQ=60)
                                                                                                                                GAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTA                     >  SRR19973065.856235/1‑150 (MQ=60)
                                                                                                                                GAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTA                     >  SRR19973065.2516302/1‑150 (MQ=60)
                                                                                                                                GAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTA                     >  SRR19973065.583547/1‑150 (MQ=60)
                                                                                                                                GAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTA                     >  SRR19973065.583352/1‑150 (MQ=60)
                                                                                                                                GAAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCAAGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGAACACAAAGGGGGAAGTGCTTACTA                     >  SRR19973065.2516299/1‑150 (MQ=60)
                                                                                                                                 AAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGACGTGCTTACTAA                    >  SRR19973065.467210/1‑150 (MQ=60)
                                                                                                                                 AAGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAA                    >  SRR19973065.469433/1‑150 (MQ=60)
                                                                                                                                  AGCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAAT                   <  SRR19973065.1766482/150‑1 (MQ=60)
                                                                                                                                   GCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATT                  <  SRR19973065.2004699/150‑1 (MQ=60)
                                                                                                                                   GCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATT                  <  SRR19973065.3013873/150‑1 (MQ=60)
                                                                                                                                   GCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATT                  <  SRR19973065.2599240/150‑1 (MQ=60)
                                                                                                                                   GCACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATT                  <  SRR19973065.2109345/150‑1 (MQ=60)
                                                                                                                                    CACGGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTA                 <  SRR19973065.318425/150‑1 (MQ=60)
                                                                                                                                       GGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGA              >  SRR19973065.2992930/1‑150 (MQ=60)
                                                                                                                                       GGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGA              >  SRR19973065.2007773/1‑150 (MQ=60)
                                                                                                                                       GGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGA              >  SRR19973065.2007530/1‑150 (MQ=60)
                                                                                                                                       GGAAAGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGA              >  SRR19973065.2007608/1‑150 (MQ=60)
                                                                                                                                           AGTGAAACCGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACA          <  SRR19973065.52455/150‑1 (MQ=60)
                                                                                                                                           AGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACA          >  SRR19973065.1974832/1‑150 (MQ=60)
                                                                                                                                           AGTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACA          >  SRR19973065.1974735/1‑150 (MQ=60)
                                                                                                                                            GTGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACAT         <  SRR19973065.683890/150‑1 (MQ=60)
                                                                                                                                             TGAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATA        <  SRR19973065.3353377/150‑1 (MQ=60)
                                                                                                                                              GAAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAA       <  SRR19973065.3040194/150‑1 (MQ=60)
                                                                                                                                               AAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAA      >  SRR19973065.3294021/1‑150 (MQ=60)
                                                                                                                                               AAAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAA      <  SRR19973065.2589734/150‑1 (MQ=60)
                                                                                                                                                AAAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAAC     <  SRR19973065.210447/150‑1 (MQ=60)
                                                                                                                                                 AAAGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACT    <  SRR19973065.2713838/150‑1 (MQ=60)
                                                                                                                                                   AGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTAC  <  SRR19973065.387340/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGCAGGCTTCAAGGTTTTTATGCATAGCATCATCGCTACCACTAACCAGAATGGAAGCGTCTGTAAGACGGTTGATAAATAAATTTGCTGGCAAACCCTACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTAC  >  CP009273/1104492‑1104788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑